LOCUS Exported 7980 bp DNA circular SYN 26-JUL-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7980) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 26, 2021 from SnapGene 5.3.2 https://www.snapgene.com COMMENT LOCUS MIGRII 6462 bp DNA circular 14-NOV-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|425052980| COMMENT VNTDBDATE|427051417| COMMENT LSOWNER| COMMENT VNTNAME|MIGRII| COMMENT VNTAUTHORNAME|auto| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTREPLTYPE|Virus COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Animal/Other Eukaryotic FEATURES Location/Qualifiers source 1..7980 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..515 /label=5'LTR misc_feature 516..1404 /label=packaging signal primer_bind 1333..1353 /label=MSCV-5' misc_feature 1455..1720 /label=hU6 promoter /note="hU6 promoter" misc_feature 2075..2380 /label=ccdB /note="ccdB" misc_feature 2433..2525 /label=iCRISPR scaffold /note="iCRISPR scaffold" misc_feature 2532..3079 /label=mPGK promoter /note="mPGK promoter" misc_feature 3080..3748 /label=Puro-2A-BFP /note="Puro-2A-BFP" misc_feature 3695..3748 /label=T2A /note="T2A" misc_feature 3749..4276 /label=Thy1.1 /note="Thy1.1" LTR 4372..4855 /label=3' LTR rep_origin 5368..6041 /direction=RIGHT /label=ColE1 misc_feature complement(6189..7046) /label=AmpR ORIGIN 1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggatccagtt tggttagtac 1441 cgggccctac gcgtccaagg tcgggcagga agagggccta tttcccatga ttccttcata 1501 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac 1561 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt 1621 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga 1681 tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttccg taatctagag 1741 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta 1801 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca 1861 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc 1921 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa 1981 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac 2041 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa 2101 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg 2161 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga 2221 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc 2281 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga 2341 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca 2401 cagccagtct gcaggtcgac catagaagac acgtttaaga gctatgctgg aaacagcata 2461 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 2521 tttttggatc caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 2581 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 2641 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 2701 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 2761 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 2821 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 2881 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct 3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 3661 ccgcaagccc ggtgccggcg gcgggtccgg aggagagggc agaggaagtc tcctaacatg 3721 cggtgacgtg gaggagaatc ctggcccaac catgggtctt ttctgcagtc accgtcctcg 3781 aggcaccatg aacccagcca tcagcgtcgc tctcctgctc tcagtcttgc aggtgtcccg 3841 agggcagaag gtgaccagcc tgacagcctg cctggtgaac caaaaccttc gcctggactg 3901 ccgccatgag aataacacca aggataactc catccagcat gagttcagcc tgacccgaga 3961 gaagaggaag cacgtgctct caggcaccct tgggataccc gagcacacgt accgctcccg 4021 cgtcaccctc tccaaccagc cctatatcaa ggtccttacc ctagccaact tcaccaccaa 4081 ggatgagggc gactactttt gtgagcttcg cgtctcgggc gcgaatccca tgagctccaa 4141 taaaagtatc agtgtgtata gagacaagct ggtcaagtgt ggcggcataa gcctgctggt 4201 tcagaacaca tcctggatgc tgctgctgct gctttccctc tccctcctcc aagccctgga 4261 cttcatttct ctgtgagtcg acctgcagcc aagctatcga taaaataaaa gattttattt 4321 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa 4381 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa 4441 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc 4501 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt 4561 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt 4621 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc 4681 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc 4741 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg 4801 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt 4861 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc 4921 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt 4981 atggacagcg cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct 5041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 5101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 5161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 5221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 5761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 5821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 5881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 6001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 6061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 6121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 6181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 6241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 7021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 7081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 7141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 7201 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 7261 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 7321 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 7381 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 7441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg 7501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 7561 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 7621 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc 7681 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc 7741 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg 7801 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag 7861 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta 7921 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa //