LOCUS       Exported                8292 bp ds-DNA     circular SYN 08-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 8, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..29
                     /locus_tag="tac promoter"
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    37..53
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             68..73
                     /locus_tag="RBS"
                     /label=RBS
                     /note="/vntifkey=21"
     CDS             82..90
                     /locus_tag="Start Codon + BamHI"
                     /label=Start Codon + BamHI
                     /note="MBP"
     misc_feature    91..1245
                     /locus_tag="MBP"
                     /label=MBP
                     /note="MBP"
     misc_feature    1246..1266
                     /locus_tag="TEV Protease Site"
                     /label=TEV Protease Site
                     /note="TEV Protease Site"
     misc_feature    1267..1269
                     /locus_tag="Serine Linker"
                     /label=Serine Linker
                     /note="Serine Linker"
     CDS             1270..1974
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    1975..1980
                     /locus_tag="Stop Codons"
                     /label=Stop Codons
                     /note="/modified_by=aaronwhiteley"
     misc_feature    1981..1989
                     /locus_tag="NotIsite"
                     /label=NotIsite
     misc_signal     1990..2016
                     /locus_tag="strepII"
                     /label=strepII
     misc_feature    2017..2019
                     /locus_tag="Stop Codon"
                     /label=Stop Codon
     misc_binding    complement(2023..2029)
                     /locus_tag="AarI Site"
                     /label=AarI Site
     misc_feature    complement(2080..2097)
                     /locus_tag="pBAD_rev_primer"
                     /label=pBAD_rev_primer
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIEA 
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHP 
                     EAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGE 
                     GRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQ 
                     HVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLY 
                     FSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      2130..2286
                     /locus_tag="rrnB_terminator"
                     /label=rrnB_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIEA 
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHP 
                     EAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGE 
                     GRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQ 
                     HVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLY 
                     FSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      2252..2295
                     /locus_tag="rrnB_T1_terminator"
                     /label=rrnB_T1_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIEA 
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHP 
                     EAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGE 
                     GRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQ 
                     HVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLY 
                     FSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      2427..2454
                     /locus_tag="rrnB_T2_terminator"
                     /label=rrnB_T2_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIEA 
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHP 
                     EAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGE 
                     GRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQ 
                     HVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLY 
                     FSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        2495..2523
                     /locus_tag="bla promoter"
                     /label=bla promoter
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIEA 
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHP 
                     EAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGE 
                     GRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQ 
                     HVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLY 
                     FSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     CDS             2565..3129
                     /locus_tag="bla'"
                     /label=bla'
     gene            complement(3789..4448)
                     /gene="CAT/CamR"
                     /locus_tag="CAT"
                     /label=CAT
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGYI 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY 
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLD 
                     RWEPELNEAIPNDERDTTMPAAMATTLRAAEVARNREWNQPVIPRYYD*"
     CDS             4542..4835
                     /locus_tag="bla'(1)"
                     /label=bla'
                     /label=bla'(1)
     rep_origin      4894..5193
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGYI 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY 
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLD 
                     RWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLL 
                     RSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE 
                     IGASLIKHW*"
     rep_origin      5427..6046
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGYI 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY 
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLD 
                     RWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLL 
                     RSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE 
                     IGASLIKHW*"
     misc_feature    6386..6408
                     /locus_tag="pGEX_3_primer"
                     /label=pGEX_3_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGYI 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY 
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLD 
                     RWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLL 
                     RSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE 
                     IGASLIKHW*"
     misc_feature    6546..6565
                     /locus_tag="pBRrevBam_primer"
                     /label=pBRrevBam_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGYI 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY 
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLD 
                     RWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLL 
                     RSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE 
                     IGASLIKHW*"
     misc_binding    6656..7005
                     /locus_tag="RP4 OriT"
                     /label=RP4 OriT
     CDS             complement(7039..8121)
                     /locus_tag="lacI"
                     /label=lacI
     promoter        complement(8122..8199)
                     /gene="lacI (mutant)"
                     /locus_tag="lacIq promoter"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     -10_signal      complement(8157..8162)
                     /locus_tag="lacIq -10"
                     /label=lacIq -10
     -35_signal      complement(8180..8185)
                     /locus_tag="lacIq -35"
                     /label=lacIq -35
     variation       8184
                     /locus_tag="C->T lacIq mutation"
                     /label=C->T lacIq mutation
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 cgctagcagg aggaattcac catgggatcc atgaaaatcg aagaaggtaa actggtaatc
      121 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
      181 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
      241 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
      301 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
      361 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
      421 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
      481 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
      541 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
      601 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
      661 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
      721 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
      781 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
      841 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
      901 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
      961 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     1021 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     1081 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     1141 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac taattcgagc
     1201 tcgaacaaca acaacaataa caataacaac aacctcggga tcgaggaaaa cctgtacttc
     1261 caaggatccg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1321 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1381 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1441 ctgcccttcg cctgggacat cctgtcgcct cagttcatgt acggctccaa ggcctacgtg
     1501 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     1561 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     1621 caagacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     1681 cccgtaatgc agaagaagac gatgggctgg gaggcctcct ccgagcggat gtaccccgag
     1741 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1801 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcaactgcc cggcgcctac
     1861 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1921 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgataa
     1981 gcggccgcaa gctggagcca tccacaattc gaaaaataat gagcaggtgc ccgggtaatg
     2041 aaagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     2101 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     2161 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggctccc
     2221 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2281 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     2341 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     2401 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     2461 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2581 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2641 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2701 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2821 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2881 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2941 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3061 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     3121 caacgttgct gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg
     3181 atactatgac tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag
     3241 tccgcaccgc tgccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     3301 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc
     3361 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt
     3421 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     3481 cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     3541 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     3601 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     3661 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     3721 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     3781 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     3841 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     3901 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     3961 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     4021 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     4081 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     4141 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     4201 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     4261 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     4321 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     4381 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     4441 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     4501 agtgatctta tttcattatg gtgaaagttg gaacctctta caaactatta actggcgaac
     4561 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4621 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4681 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4741 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4801 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4861 tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4921 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4981 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5041 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     5101 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5161 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     5221 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5281 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat
     5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6061 cctggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     6241 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt catggctgcg
     6301 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6361 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6421 tcaccgaaac gcgcgaggca gaaggagatg gcgcccaaca gtcccccggc cacggggcct
     6481 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6541 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     6601 gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca tcgatttccg
     6661 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     6721 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     6781 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     6841 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     6901 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     6961 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aaatcgatgc atgctaactc
     7021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     7141 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     7201 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     7261 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     7321 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     7381 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     7441 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     7501 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     7561 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     7621 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     7681 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     7741 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     7801 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     7861 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     7921 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     7981 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     8041 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     8101 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     8161 tgccataccg cgaaaggttt tgcaccattc gatggtgtcc tggcacgaca ggtttcccga
     8221 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc
     8281 ccaggcgagc tg
//
