LOCUS       Exported                9281 bp ds-DNA     circular SYN 28-FEB-2025
DEFINITION  003_17D_CBC_CHyMErA_contig.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 9, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      922..1510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1798..1819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1834..1864
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1872..1888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1896..1912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1933..1951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1979..2205
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2206..2386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2433..2558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3051..3284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3469..3513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3722..3946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3952..4200
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     gap             4202..4221
                     /estimated_length=20
     misc_feature    4222..4307
                     /label=SpCas9 tracrRNAv1 (Dang et al., 2015)
     misc_feature    4308..4327
                     /label=AsCas12a DR
     gap             4328..4350
                     /estimated_length=23
     gap             4364..4375
                     /estimated_length=12
     CDS             complement(4410..4433)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4616..4634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4653..4671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4690..4708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4727..4745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4764..4782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4801..4819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4838..4856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4875..4893
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4972..5089
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5158..5668
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             5690..6286
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             6296..6352
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6353..7096
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7112..7700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7583..7594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7772..8005
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8083..8204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8244..8379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8400..8418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8428..8444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8586..9041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9067..9171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9172..9281,1..751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
       61 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
      781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
      841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1681 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     1801 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1861 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     1921 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     1981 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2041 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2101 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2161 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2221 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2281 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2341 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2401 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2461 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2521 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2581 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2641 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2701 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     2761 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     2821 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     2881 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2941 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3001 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3061 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3121 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3181 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3241 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3301 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3361 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3421 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3481 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3541 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3601 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3661 acccacctcc caaccccgag gggaccctgc aggatttaaa tccgagggga cccagagaat
     3721 tccatagagc ccaccgcatc cccagcatgc ctgctattgt ctacccaatc ctcccccttg
     3781 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     3841 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     3901 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagaatt cgagggccta
     3961 tttcccatga ttccttcata tttgcatata cgatacaagg cggttagaga gataattaga
     4021 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     4081 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     4141 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaggtacc
     4201 gnnnnnnnnn nnnnnnnnnn ngtttcagag ctatgctgga aacagcatag caagttgaaa
     4261 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgctaa tttctactct
     4321 tgtagatnnn nnnnnnnnnn nnnnnnnnnn ttttttgaaa gctnnnnnnn nnnnncaatt
     4381 gttaattaag gcgcgccagc gctgatatcc ttgtcatcgt catccttgta atcgatgtca
     4441 tgatcatatg tatcaccgtc atggtctttg tagtccatgt taacgctaga ggatccagcc
     4501 tccgcggagg ttggatcggt cccggtgtca tctatggagg tcaaaacagc gtggatggcg
     4561 tctccaggcg atctgacggt tcactaaacg agctctgctt atataggggg taccttctct
     4621 atcactgata gggagctcga gacgtgtcga cttctctatc actgataggg agacgttcta
     4681 gacgtacctt ctctatcact gatagggagc tcgagacgtg tcgacttctc tatcactgat
     4741 agggagacgt tctagacgta ccttctctat cactgatagg gagctcgaga cgtgtcgact
     4801 tctctatcac tgatagggag acgttctaga cgtaccttct ctatcactga tagggagctc
     4861 gagacgtgtc gacttctcta tcactgatag ggagacgtgc ggccagcttg gatccctcga
     4921 ggttaacacc ggtttctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     4981 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     5041 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     5101 cagggacagc agagatccac tttggcgccg gctcgagatt taaatcctgc aggcgagggg
     5161 gcccgggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     5221 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     5281 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     5341 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     5401 agccgcagct cacactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     5461 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     5521 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     5581 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     5641 tgaccgaatc accgacctct ctccccaggg ggatccaccg gagcttacca tgaccgagta
     5701 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5761 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5821 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5881 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     5941 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6001 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6061 cctggccacc gtcggtgttt cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6121 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6181 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6241 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccggat ccggcgcaac
     6301 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgtctagact
     6361 ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg
     6421 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     6481 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     6541 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     6601 gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     6661 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     6721 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     6781 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     6841 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     6901 agccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     6961 aagcggcggg ccggccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     7021 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
     7081 catgctcccc gggtaaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     7141 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7201 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7261 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7321 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7381 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7441 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7501 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7561 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7621 gctgccggct ctgcggcctc ttccgcgtgt tcgccttcgc cctcagacga gtcggatctc
     7681 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     7741 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgtaga
     7801 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7981 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     8041 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8221 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8341 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     8401 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     8461 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     8521 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     8581 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8701 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9001 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     9061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc a
//
