1426 LOCUS ADDGENE_1426 3052 bp DNA circular SYN 01/03/2005 DEFINITION pAST_18b complete plasmid sequence KEYWORDS . SOURCE pAST_18b ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1426 at www.addgene.org FEATURES Location/Qualifiers source 1..3052 /organism="Addgene plasmid pAST_18b" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature complement(461..479) /label="M13r_primer" promoter complement(514..543) /label="lac_promoter" misc_feature complement(610..636) /label="lacI.C" rep_origin complement(852..1471) /label="pBR322_origin" CDS complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" misc_feature complement(2778..2807) /label="SupTrp.3'" misc_feature complement(2822..2851) /label="SupTrp.5'" promoter complement(2894..2922) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGGTCG 421 ACTCTAGAGG ATCCCCGGGT ACCGAGCTCG AATTCGTAAT CATGGTCATA GCTGTTTCCT 481 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 541 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 601 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 661 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 721 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 781 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 1021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT 1081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 1141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 1201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 1261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 1321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 1381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 1441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1561 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1621 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1681 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 1741 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 1801 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 1861 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 1921 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 1981 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2041 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2101 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2161 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2221 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2281 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2341 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2401 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2461 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATACAATTT TCAGAATACG 2521 TTTTTTGTGG GCTTGGGTAT ATTGTTTTTA ATGTTATACT TGCAGTCGTG AAATTTGATT 2581 TTCAAATTTG TAGAAAAATC AAGAAAATAA TTGCAACATT CGCTTGTGTC AAAAACCAAT 2641 TTCAACAAAT TTTCGTGTGA GAAATACATT ACCAGAAGGC ATTTTTTCAC ACGATTAGCA 2701 TTTTGGACTA CTTTATTAAA TTTTTGCGTG TAATTTTGAA TTAAATTGTA TTATATTACT 2761 ACTTAAAAAA CAAAAAATTT GACCACTGAG CGGATCGAAC GCCCAACCTT TCGATCTAGA 2821 GTCGAACGCG CTACCATTGC GCCAAGCAGT CATGTTATTC TCTCTTGTCA TTATTGAAGC 2881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 3001 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1427 LOCUS ADDGENE_1427 3052 bp DNA circular SYN 01/03/2005 DEFINITION pAST_19a complete plasmid sequence KEYWORDS . SOURCE pAST_19a ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1427 at www.addgene.org FEATURES Location/Qualifiers source 1..3052 /organism="Addgene plasmid pAST_19a" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..398) /label="lacZ_a" misc_feature complement(461..479) /label="M13r_primer" promoter complement(514..543) /label="lac_promoter" misc_feature complement(610..636) /label="lacI.C" rep_origin complement(852..1471) /label="pBR322_origin" gene complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" CDS complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature complement(2778..2807) /label="SupTrp.3'" misc_feature complement(2822..2851) /label="SupTrp.5'" promoter complement(2894..2922) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGGAT 421 CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 481 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 541 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 601 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 661 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 721 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 781 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 1021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 1081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 1141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 1201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 1261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 1321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 1381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 1441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1561 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1621 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1681 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 1741 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 1801 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 1861 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 1921 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 1981 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2041 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2101 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2161 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2221 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2281 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2341 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2401 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2461 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATACAATTT TCAGAATACG 2521 TTTTTTGTGG GCTTGGGTAT ATTGTTTTTA ATGTTATACT TGCAGTCGTG AAATTTGATT 2581 TTCAAATTTG TAGAAAAATC AAGAAAATAA TTGCAACATT CGCTTGTGTC AAAAACCAAT 2641 TTCAACAAAT TTTCGTGTGA GAAATACATT ACCAGAAGGC ATTTTTTCAC ACGATTAGCA 2701 TTTTGGACTA CTTTATTAAA TTTTTGCGTG TAATTTTGAA TTAAATTGTA TTATATTACT 2761 ACTTAAAAAA CAAAAAATTT GACCACTGAG CGGATCGAAC GCCCAACCTT TCGATCTAGA 2821 GTCGAACGCG CTACCATTGC GCCAAGCAGT CATGTTATTC TCTCTTGTCA TTATTGAAGC 2881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 3001 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1428 LOCUS ADDGENE_1428 5808 bp DNA circular SYN 01/03/2005 DEFINITION pDD16_01 complete plasmid sequence KEYWORDS . SOURCE pDD16_01 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1428 at www.addgene.org FEATURES Location/Qualifiers source 1..5808 /organism="Addgene plasmid pDD16_01" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 3722..3750 /label="Amp_promoter" gene 3792..4652 /label="Ampicillin" /gene="Ampicillin" CDS 3792..4652 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4807..5426 /label="pBR322_origin" misc_feature 5642..5668 /label="lacI.C" promoter 5735..5764 /label="lac_promoter" misc_feature 5799..5817 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG 3361 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 3421 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 3481 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 3541 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG 3601 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 3661 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 3721 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 3781 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 3841 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 3901 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 3961 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 4021 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 4081 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 4141 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 4201 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 4261 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 4321 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 4381 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 4441 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 4501 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 4561 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 4621 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 4681 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 4741 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 4801 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 4861 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 4921 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT 4981 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 5041 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 5101 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 5161 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG 5221 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 5281 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 5341 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 5401 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 5461 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 5521 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 5581 AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT 5641 AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA 5701 ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 5761 TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1429 LOCUS ADDGENE_1429 3042 bp DNA circular SYN 01/03/2005 DEFINITION pICT19h complete plasmid sequence KEYWORDS . SOURCE pICT19h ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1429 at www.addgene.org FEATURES Location/Qualifiers source 1..3042 /organism="Addgene plasmid pICT19h" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature complement(700..729) /label="SupTrp.3'" misc_feature complement(744..773) /label="SupTrp.5'" misc_feature complement(817..835) /label="M13r_primer" promoter complement(870..899) /label="lac_promoter" misc_feature complement(966..992) /label="lacI.C" rep_origin complement(1208..1827) /label="pBR322_origin" CDS complement(1982..2842) /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(1982..2842) /label="Ampicillin" /gene="Ampicillin" promoter complement(2884..2912) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTTCGCGA GCTCGAGATC 421 TAGATACAAT TTTCAGAATA CGTTTTTTGT GGGCTTGGGT ATATTGTTTT TAATGTTATA 481 CTTGCAGTCG TGAAATTTGA TTTTCAAATT TGTAGAAAAA TCAAGAAAAT AATTGCAACA 541 TTCGCTTGTG TCAAAAACCA ATTTCAACAA ATTTTCGTGT GAGAAATACA TTACCAGAAG 601 GCATTTTTTC ACACGATTAG CATTTTGGAC TACTTTATTA AATTTTTGCG TGTAATTTTG 661 AATTAAATTG TATTATATTA CTACTTAAAA AACAAAAAAT TTGACCACTG AGCGGATCGA 721 ACGCCCAACC TTTCGATCTA GAGTCGAACG CGCTACCATT GCGCCAAGCA GTCATGTTAT 781 TCTCTCTTGT CGACCTGCAG CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 841 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 901 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 961 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 1021 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 1081 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 1141 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 1201 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 1261 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 1321 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 1381 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 1441 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 1501 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 1561 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 1621 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 1681 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 1741 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 1801 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 1861 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 1921 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 1981 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 2041 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 2101 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 2161 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 2221 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 2281 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 2341 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 2401 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 2461 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 2521 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 2581 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 2641 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 2701 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 2761 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 2821 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 2881 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 2941 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA 3001 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1430 LOCUS ADDGENE_1430 3023 bp DNA circular SYN 01/03/2005 DEFINITION pICT19r complete plasmid sequence KEYWORDS . SOURCE pICT19r ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1430 at www.addgene.org FEATURES Location/Qualifiers source 1..3023 /organism="Addgene plasmid pICT19r" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..398) /label="lacZ_a" misc_feature 433..462 /label="SupTrp.5'" misc_feature 477..506 /label="SupTrp.3'" misc_feature complement(798..816) /label="M13r_primer" promoter complement(851..880) /label="lac_promoter" misc_feature complement(947..973) /label="lacI.C" rep_origin complement(1189..1808) /label="pBR322_origin" gene complement(1963..2823) /label="Ampicillin" /gene="Ampicillin" CDS complement(1963..2823) /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(2865..2893) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCCGGGGATC CGTCGACAAG 421 AGAGAATAAC ATGACTGCTT GGCGCAATGG TAGCGCGTTC GACTCTAGAT CGAAAGGTTG 481 GGCGTTCGAT CCGCTCAGTG GTCAAATTTT TTGTTTTTTA AGTAGTAATA TAATACAATT 541 TAATTCAAAA TTACACGCAA AAATTTAATA AAGTAGTCCA AAATGCTAAT CGTGTGAAAA 601 AATGCCTTCT GGTAATGTAT TTCTCACACG AAAATTTGTT GAAATTGGTT TTTGACACAA 661 GCGAATGTTG CAATTATTTT CTTGATTTTT CTACAAATTT GAAAATCAAA TTTCACGACT 721 GCAAGTATAA CATTAAAAAC AATATACCCA AGCCCACAAA AAACGTATTC TGAAAATTGT 781 ATCGATGAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 841 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 901 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 961 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 1141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2821 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 2881 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 2941 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 3001 GCGTATCACG AGGCCCTTTC GTC // 1431 LOCUS ADDGENE_1431 7236 bp DNA circular SYN 01/03/2005 DEFINITION pOK11_34 complete plasmid sequence KEYWORDS . SOURCE pOK11_34 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1431 at www.addgene.org FEATURES Location/Qualifiers source 1..7236 /organism="Addgene plasmid pOK11_34" /mol_type="other DNA" misc_feature 49..73 /label="myo2.delta-90" misc_feature 140..163 /label="myo2.5'" misc_feature 237..278 /label="SynIVS.A" CDS 315..3479 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 315..341 /label="SV40.NLS" gene 432..3476 /label="lacZ" /gene="lacZ" promoter 5150..5178 /label="Amp_promoter" CDS 5220..6080 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5220..6080 /label="Ampicillin" /gene="Ampicillin" rep_origin 6235..6854 /label="pBR322_origin" misc_feature 7070..7096 /label="lacI.C" promoter 7163..7192 /label="lac_promoter" misc_feature 7227..7245 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGCCTT GGTCGACTCT AGACACTTTT 61 CAGCTACCTA GATACATGGA TATCCCCGCC TCCCAATCCA CCCACCCAGG GAAAAAGAAG 121 GGCTCGCCGA AAATCAAAGT TATCTCCAGG CTCGCGCATC CCACCGAGCG GTTGACTTCT 181 CTCCACCACT TTTCATTTTA ACCCTCGGGG TACGGGATTG GCCAAAGGAC CCAAAGGTAT 241 GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCcttCT TGGAGGGTAC 301 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTG GGTGAAGACC 361 AGAAACAGCA CCTCGAACTG AGCCGCGATA TTGCCCAGCG TTTCAACGCG CTGTATGGCG 421 AGATCGATCC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 481 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 541 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCTTTGCC TGGTTTCCGG 601 CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC GATACTGTCG 661 TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC AACGTGACCT 721 ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT TGTTACTCGC 781 TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT ATTTTTGATG 841 GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC GGCCAGGACA 901 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 961 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 1021 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1081 TCCATGTTGC CACTCGCTTT AATGATGATT TCAGCCGCGC TGTACTGGAG GCTGAAGTTC 1141 AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG CAGGGTGAAA 1201 CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG CGTGGTGGTT 1261 ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG AGCGCCGAAA 1321 TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG CTGATTGAAG 1381 CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG CTGCTGCTGA 1441 ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT CTGCATGGTC 1501 AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT GATGAAGCAG AACAACTTTA 1561 ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT 1621 ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC 1681 GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC 1741 AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA TCAGGCCACG 1801 GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC CGCCCGGTGC 1861 AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG ATGTACGCGC 1921 GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA AAATGGCTTT 1981 CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG ATGGGTAACA 2041 GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT TTACAGGGCG 2101 GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC GGCAACCCGT 2161 GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC TGTATGAACG 2221 GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC GGAAGCAAAA CACCAGCAGC 2281 AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC 2341 GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA 2401 GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC 2461 TACCGCAGCC GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG 2521 CGACCGCATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA GCAGTGGCGT CTGGCGGAAA 2581 ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC ACCAGCGAAA 2641 TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG TCAGGCTTTC 2701 TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC GATCAGTTCA 2761 CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT GACCCTAACG 2821 CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT 2881 GCACGGCAGA TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC 2941 AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG 3001 CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA 3061 ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA 3121 ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA 3181 TGTATACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG CTGCGGGACG CGCGAATTGA 3241 ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA CATCAGCCGC TACAGTCAAC 3301 AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC GGAAGAAGGC ACATGGCTGA 3361 ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC CTGGAGCCCG TCAGTATCGG 3421 CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 3541 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 3601 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 3661 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 3721 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 3781 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 3841 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 3901 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 3961 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 4021 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 4081 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 4141 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 4201 TACATTCCCA ATATACCAAA CATAACTGTT TAAAATTAAA CATTTTTCTA AATTTTATAT 4261 GATTTCTTTT AAATTTGCAA AAATTACTTA AATTTGAATT CCCGCGCAAA TGAGTGACTT 4321 CATTTTCTGC ATTATTGTGT TTTCCGGCTA TATTAATAGG TATTTGTTTG TGTTTTTCTT 4381 TATTTTATGA TTCGAACTCC AATTTGTAAA TTTTCGAACA TATTTCCCTA AAGAAAAAAT 4441 ATGATTAATC TGGAAAAATT GGAAAATTAT TTTTCAAATA AAAAACAAAG AAAAAAATGA 4501 AGAAAAACCT ATTAGTTTGG CCATAAAACG CAAAAATGTC GAAAATGACG TCACTCATCT 4561 GCGCGGGAAA TCAAGAATAA TTCGGCCTTT TTTATTTTTT TGGAAAATCG TAAAACATTT 4621 AGAAAAATTT TTTAATAGTT ATATGGGACT TATTCTGCAT TTAGGGCAAA AGCCAGAGAC 4681 TACTCCACCG TTAACATGAA TTATGAATAT TATTGCGACA AGACCCAAAC ATTGATAAAC 4741 CCAAATCTAG CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 4801 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 4861 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 4921 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 4981 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 5041 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 5101 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5161 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5221 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5281 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5341 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5401 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5461 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5521 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5581 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5641 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5701 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 5761 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 5821 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 5881 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 5941 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 6001 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 6061 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6121 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6181 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6241 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6301 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6361 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6421 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6481 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6541 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6601 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6661 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6721 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 6781 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 6841 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 6901 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 6961 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 7021 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 7081 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7141 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7201 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1432 LOCUS ADDGENE_1432 11791 bp DNA circular SYN 01/03/2005 DEFINITION pPD10_46 complete plasmid sequence KEYWORDS . SOURCE pPD10_46 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1432 at www.addgene.org FEATURES Location/Qualifiers source 1..11791 /organism="Addgene plasmid pPD10_46" /mol_type="other DNA" rep_origin complement(170..789) /label="pBR322_origin" CDS complement(944..1804) /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(944..1804) /label="Ampicillin" /gene="Ampicillin" promoter complement(1846..1874) /label="Amp_promoter" rep_origin complement(2846..3152) /label="f1_origin" misc_feature 3657..3673 /label="M13f20_primer" misc_feature complement(3528..3671) /label="lacZ_a" misc_feature 5398..5428 /label="unc54.enh" CDS complement(6293..6973) /label="Orf1" /translation="MKLNNEDGKTKFHVKRALRSDTGTYIIKAENENGTDTAEVKVTV LDHPSSPRGPLDVTNIVKDGCDLAWKEPEDDGGAEISHYVIEKQDAATGRWTACGESK DTNFHVDDLTQGHEYKFRVKAVNRHGDSDPLEAREAIIAKDPFDRADKPGTPEIVDWD KDHADLKWTPPADDGGAPIEGYLVEMRTPSGDWVPAVTVGAGGALDGSQSRFGRRCGS NALLELDG" CDS 9057..9869 /label="Orf2" /translation="MNNELESGSDLDSCIRKIFTMNSWSKVSWLRNNTIRRIRRISSS SLVTSRNSELIFFSLLKIWNSCCFLISWNNESFFPSSILILLFNDESFNWCTTIVWSI PCKINMILIPVHQFRLVNFSWNLVWVLGEDCGVGCEWIGFSLLVHSSDTEFVFVSGLK IWNCGLCCFTSSTWDPSSSCQIHLFDFIMINWSTSVIFWYFPLQICAIFVDIRHFQRS QWARWFSEHNDLNGMFVASRSVGSSKSVQTSMLASCSTEHKSGLVVFAFNLK" misc_feature complement(11570..11588) /label="M13r_primer" promoter complement(11623..11652) /label="lac_promoter" misc_feature complement(11719..11745) /label="lacI.C" ORIGIN 1 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 61 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 121 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 181 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 241 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 301 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 361 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 421 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 481 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 541 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 601 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 661 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 721 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 781 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 841 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 901 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 961 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 1021 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 1081 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 1141 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 1201 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 1261 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 1321 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 1381 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 1441 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 1501 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 1561 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 1621 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 1681 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 1741 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 1801 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 1861 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 1921 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 1981 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 2041 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 2101 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 2161 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 2221 GGAGAAAATA CCGCATCAGG AANTTCCAAC ATCCAATAAA TCATACAGGC AAGGCAAAGA 2281 ATTAGCAAAA TTAAGCAATA AAGCCTCAGA GCATAAAGCT AAATCGGTTG TACCAAAAAC 2341 ATTATGACCC TGTAATACTT TTGCGGGAGA AGCCTTTATT TCAACGCAAG GATAAAAATT 2401 TTTAGAACCC TCATATATTT TAAATGCAAT GCCTGAGTAA TGTGTAGGTA AAGATTCAAA 2461 CGGGTGAGAA AGGCCGGAGA CAGTCAAATC ACCATCAATA TGATATTCAA CCGTTCTAGC 2521 TGATAAATTC ATGCCGGAGA GGGTAGCTAT TTTTGAGAGG TCTCTACAAA GGCTATCAGG 2581 TCATTGCCTG AGAGTCTGGA GCAAACAAGA GAATCGATGA ACGGTAATCG TAAAACTAGC 2641 ATGTCAATCA TATGTACCCC GGTTGATAAT CAGAAAAGCC CCAAAAACAG GAAGATTGTA 2701 TAAGCAAATA TTTAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 2761 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 2821 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG 2881 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 2941 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC 3001 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 3061 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 3121 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA CTATGGTTGC 3181 TTTGACGAGC ACGTATAACG TGCTTTCCTC GTTAGAATCA GAGCGGGAGC TAAACAGGAG 3241 GCCGATTAAA GGGATTTTAG ACAGGAACGG TACGCCAGAA TCCTGAGAAG TGTTTTTATA 3301 ATCAGTGAGG CCACCGAGTA AAAGAGTCTG TCCATCACGC AAATTAACCG TTGTCGCAAT 3361 ACTTCTTTGA TTAGTAATAA CATCACTTGC CTGAGTAGAA GAACTCAAAC TATCGGCCTT 3421 GCTGGTAATA TCCAGAACAA TATTACCGCC AGCCATTGCA ACAGGAAAAA CGCTCATGGA 3481 AATACCTACA TTTTGACGCT CAATCGTCTG GAANTTCCAT TCGCCATTCA GGCTGCGCAA 3541 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 3601 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 3661 AACGACGGCC AGTGCCAAGC TTGGCTGCAG TGAGTATTTT CGGTTTTTCA CTGGTCTCTG 3721 TAAAAAGAGT AATGCAAAGG GCAAGTTAAC TAGGTCGTAA ATGTATTGAT TTGCTTAAAA 3781 TCTGAAGATC TAGTGGTGAA CCGTGGAAGA TTATCAAGAG GAGGCTGAAG ATCTGTTTAA 3841 GAACCATTAA TCAAACTGGT ATTCTATTTT CACTGGTTGT ATGTAAACAT TCTATCTTAT 3901 TCCTTTTATC ACTGTTCTGC ACTTTCCTAT AAAAAAAGTT GACCGACCGT ACTCTCTGAA 3961 TTCATTTTTC CCGATCTTAC CAACTCCCGA TCTATCTCTA TCCCTGGTTT TTTCTTCGTG 4021 CTCCAATGGA ATTCTTGAGA CTTCCACTAT CTTCTCTGGC ACCCTCCACT ACGCGTAGGC 4081 GTCTCTCGCT TCGTGTATTC CCGGGAAGCC GGGTTCCCGT CTCTCCCGCC GCTGCCGCTG 4141 CCGCACACAG CTTTACACCT CGTAGAATCC CCAAAGAGGG GCGTGGCTTG CGGGTGCCAA 4201 CATCCTCCTG CCGAGGAAGA AGCAGGCACT CATCACTCGC ATCATCAACC TCGACTAAGG 4261 GAGCACCACA TTAGTTTTGA GGTAGAGAAA CCATTTGAAA GAAGCGAGAA ATCATGGAGC 4321 ACGAGAAGGA CCCAGGATGG CAATATCTCC GCCGTACCAG AGAGCAGGTT TTGGAGGTGA 4381 GTATTTATTT TTTATTTATT TATTTTTCTT TCATTTGTCT TTATTGTTAT GCTCATTTCT 4441 ATAGAGTAAA TCGTATAGTA CTCCATAAGT ATCTAATTTT AACAAAACTG AGCATTTATT 4501 TTTGAAATTC TAGATTTTTA ACATACAACT GAGTTAAAGT CTGCCATTTT ACACTTATAG 4561 ATTTTTTAAA TTCAGGTAGA AGATTTGAGC TTGCCTTGTA ATCCTTGTAA TCAATGTTTA 4621 TTTCTGTTTT CTAATTTTTC ATAGATTTAG CTTCAAGATC ATCTTTCCCT TGAAATCATT 4681 GAAATCTTCC TTTCTTAAAT TTTTGCATTG CACTTGGAAT TTTGTGACAC ATTGTGCTGC 4741 AGTAGTACTG TATTTTGAGA CGGCACATTT ATTTTGACTA CACCACAAGC TTAACCTTGA 4801 TTTTACTTTT TGATTCCATT CCATTCATGG GTCTAAAGTG TGAAGCGGTT TAGACCATTT 4861 TTCAACACAA GTTGGTATTC TTTAGAAGTG ATATATTTCT GAATTCTGAT CTCTTTTGTA 4921 CCATTATTGT AATTTCAGGA TCAATCTAAG CCTTATGACT CCAAGAAGAA CGTCTGGATC 4981 CCAGATCCAG AAGAGGGATA CCTTGCCGGA GAAATCACCG CCACCAAGGG AGACCAGGTC 5041 ACCATCGTCA CCGCTCGCGA AATGAGGTTC GTTTTTAAGC TAATGATTAC GGTTTTCAAT 5101 GTAGTGTGAT TCAGGTCACC CTCAAGAAGG AGTTGGTTCA AGAAATGAAT CCACCAAAGT 5161 TCGAGAAGAC CGAAGACATG TCCAACTTGT CTTTCCTCAA CGACGCCTCT GTCCTCCATA 5221 ACTTGCGTTC CCGTTACGCT GCTATGCTCA TCTACGTAAG TTTCATTGAA ACTTAAAGTT 5281 AAGCATTGAG AATCATTCAT ATTCTTCTTC TAAATTCCCA TAAAATCCCG AAACTCCTTC 5341 CCTCTATCTT CTTTTTCTTC TCGTTTTCAA ATGTTTCTCT CTATCCCATT CTCTCATCAA 5401 TTGAGTGGGA TGAGGCTATC TCTGCCTCTC TTCTGAATCT CTGAACCATC TTACATTACA 5461 CTGTGGATGA CGAGCCCCAC AGGCTCCCTT GCATCAGATA CTGCCATTGG GGATGGCAAA 5521 GAAGAGAGAA GGTATTGTGA GGATATATTT TTCTAAGAAA AAACGTTTGA AGAAAAGAAG 5581 ATGAAGAAGA TCTGCTTGAT TCATTGCACA AGTTAGAAGT AACAGGGGTC TATATTTCGA 5641 AGAACTTAAA GGGAATGCAA CTGAACATAA AATTAAACAA AGGGATTGAA TCCTGCAGTA 5701 TTTAAAAATC GAAACTAGAA TGTTTCCTAT TTCAGACCTA CTCCGGACTT TTCTGCGTTG 5761 TCATCAACCC ATACAAGCGT CTCCCAATCT ACACCGACTC TTGCGCCCGT ATGTTCATGG 5821 GAAAGAGAAA GACAGAAATG CCACCACATT TGTTCGCTGT CTCTGACGAA GCCTACCGTA 5881 ACATGCTCCA AGACCACGAG AACCAGTCTA TGCTCATTAC CGGAGAATCT GGAGCCGGAA 5941 AGACTGAGAA CACCAAGAAG GTTATTTGCT ACTTCGCCGC TGTCGGTGCC TCCCAACAGG 6001 AAGGTGGAGC TGAAGTTGAT CCAAATAAGA AGAAGGTCAC CCTTGAGGAC CAAATCGTCC 6061 AGACCAATCC AGTACTTGAA GCCTTCGGTA ACGCCAAGAC TGTCCGTAAC AACAACTCAT 6121 CCCGTTTCGG AAAGTTCATC CGTATCCACT TCAACAAGCA CGGACGCCTC GCATCTTGCG 6181 ATATCGAGCA TTGTGAGTTA CTTTTTGATT TATATTCTAG CATAGCTCAA TAAAAATTAT 6241 GCAACTATTG TTTAAACTAT AAATTTTGCA GACTTGCTCG AGAAATCTCG TGTCATCCGT 6301 CAAGCTCCAG GAGAGCGTTG CTACCACATC TTCTACCAAA TCTACTCTGA CTTCCGTCCA 6361 GAGCTCCACC GGCTCCAACC GTGACAGCTG GTACCCAGTC TCCTGATGGA GTTCTCATTT 6421 CAACGAGATA TCCTTCGATT GGAGCACCTC CATCATCAGC TGGTGGAGTC CACTTGAGAT 6481 CTGCATGATC CTTGTCCCAG TCCACAATTT CTGGAGTTCC TGGCTTATCA GCACGATCGA 6541 ATGGATCCTT GGCGATAATA GCTTCACGAG CCTCCAATGG ATCCGAATCT CCATGTCTGT 6601 TAACAGCCTT GACACGGAAT TTATATTCAT GCCCTTGAGT CAAATCGTCA ACGTGGAAGT 6661 TGGTATCCTT GCTCTCTCCG CAAGCAGTCC ATCTGCCAGT GGCAGCATCT TGCTTTTCAA 6721 TGACATAGTG ACTGATTTCA GCTCCTCCAT CATCTTCTGG TTCCTTCCAT GCAAGATCAC 6781 ATCCATCCTT GACAATATTA GTGACATCGA GAGGTCCACG TGGGCTTGAT GGATGATCAA 6841 GAACAGTAAC CTTCACTTCA GCAGTGTCAG TTCCATTCTC GTTCTCTGCC TTGATGATAT 6901 AGGTTCCTGT ATCCGAACGC AAAGCTCTCT TCACATGGAA TTTAGTCTTG CCGTCTTCAT 6961 TGTTCAACTT CATACGATCA TCAGATTCGA CTGGTGTTCC TTCGAAAGTC CAAGTAATTG 7021 TTGGAGTTGG TTCACCACTG ACTGGAATGT TCAATGAGAA GTCTTGTCCA GCCTTGACCT 7081 TGATTTCTTG AATCGAGTTA CGATCGATGA CTGGTGGAAC TATAATTTAA TTCAATGATT 7141 ATTAGTAATT GATTTAGACT CTTACCATTT CTAGCCTTTG CAACAGCTGA TGCTGAATCA 7201 GATGGATCTC CCAATCCTGC CTTGTTCTTG GCACGGATTC TGAATTCGTA CTTTGATCCT 7261 TCCTTGAGAT TTCCAACAGT AGCATTCGTT TGTCCAGCTG GAACATGAGC AACGTCATTC 7321 CAGAATGGCG AGAACTCGTC CTTCATCTCA ACAACGTATT CCTCGATTGG AGCACCACCG 7381 TCGTTTGCTG GTGGCTTCCA TTCAAGGTCA ACATGATCCT TATCCCAATC AGTAATTTCA 7441 GGAGCATTTG TCTTTCCTGG CTTGTCAAAT GGATCTTTGG CAAGTGTGGT TCCGAAGGTC 7501 TCCAATGGAT CGGACTCTCC TTCAGCATTG ACGGCAGCGA CACGGAACTG AAAATCAAAA 7561 TGTTGTAGGC AATTGAGTTC AAGATTAAAA AATTCTCACT TTATATTCAT GTCCAGGAAT 7621 AAGACCGTCA ACAACAGCTG TAGTCTTATC TCCAGCGACC TTTGCAGCTG GAACCCATCT 7681 TCCACTTGCA GTATCGTACT TTTCGATCAC ATAGTTTTCA ATTGGAATAC CTCCATCATC 7741 ATCTGGTGCA CGCCAATTCA AAGTGACATG ATCACCATGA ACATCGGAAA CATCTAATGG 7801 ACCATTTGGA GAAGTTGGCT TGTCTGAAAA TTTAAAATAT AACCAAATTA ATTGAAGAAA 7861 ACTAATGCTC ACCAATAACA TTGATCTTAA CAGTTGCTTC ATCTTCTCCA TTTGCATTGA 7921 CAGCTTTGAT AGTGAAAGTT CCACTGTCTC CACGTTCCAT TTGCTTCACA ACCAGCTTTG 7981 ATTGGTATTC TGGGTTATCA AGCTTGACGC GTCCTCCATT TCTGATTTCC CTGTTGTCTG 8041 GCCAATGCCA TGTGACTGTT GGTGCAGGTT CTCCTGATAC TGGAACATCG AAGGAAATTG 8101 ATTGACCAGC CTTGATAGTC AGATTCTTGA GAGCATCTCT GTCGATGTGT GGCTTCACTG 8161 AAAATTAAAA AATAGCACTT TTCCTCAAAA TTAAACAATC AACTCACAGT TTCTTGGTTT 8221 AGCAATAACA GCCTTGCTAG CATCAGATGG ATCAGATGGG CCTCCCTTGT TAACAGCAAC 8281 AACACGGAAT TCGTATTCCT CATTTGGTGT GAGGTCTGGA ACGGTTGCAG TTGTCTGACC 8341 GCCAGGAACA GTGATGGCTG GTTCCCATCT GCAAAATTAG AATTGGCAAA ACTTCAAATT 8401 AAAAAAAAAC GATACCTTCC GTATTTGGTT CTCTTCTCAA TTTGGTACTC CTCAATTGGA 8461 GCGCCGCCAT CAGAAAGTGG TGGATCCCAC TTGAGATCAA CATGATCAGA ATCCCAATCA 8521 GTTGGCTCTG GACGACCTGG TCTATCTGGA CGATCAAATG GATTTTTAGC CAAAATATCG 8581 GTTTCAGTTT CCAATGGATC AGAAGGTCCT TCGCTGTTGA CAGCCTTGAC ACGGAACTTG 8641 TAATGATCTC CCTTGTTGAG TGAATCGACT GTGAAGTGGG TATCTCCACT ACGTCCACAT 8701 GGAACCCAGA TTCCGTCCTT GGTGTTCATC TTTTCAATTT CATAGAACTC AATTGGCTCT 8761 CCTCCGTCAT CCTCTGGTGG TTTCCAGTCA AGAGTTGCAC GATCTTCGAA GACATCAGTT 8821 ACCTCGATTG GTCCCTTTGG TTTCGTTGGC TTGCTTTTTA CCTTGATATT GATAGTGACA 8881 CTGTCAGTTC CATTAATGTT GGTTGCAGTG ATGGTATATT TTCCACTTTG CTTACGAAGT 8941 GCCTTTGGCA AAGCAAATCT CGAGATGTAT GGCTCATTTT CAATTTGAGC CTTGCTCTCT 9001 CCGATTCCTT TTCCATTGAA TGACCATGTT ACATCTGGTG CTGGCTCACC ATCAATATGA 9061 ACAATGAACT TGAGAGTGGC TCCGACCTTG ACAGTTGTAT CAGAAAGATC TTCACGATGA 9121 ATTCTTGGAG CAAGGTTTCT TGGCTTCGCA ACAACACGAT CAGAAGGATC AGAAGGATCT 9181 CCAGTTCCAG CCTTGTTACG AGCAGAAATT CTGAATTGAT ATTCTTCTCC CTCCTTAAGA 9241 TTTGGAACAG TTGCTGTTTT CTGATCTCCT GGAACAACGA GAGCTTCTTC CCATCGTCCA 9301 TTCTTATCCT TCTTTTCAAT GACGAAAGCT TCAATTGGTG CACCACCATC GTCTGGAGCA 9361 TTCCATGCAA GATCAACATG ATCCTTATCC CAGTCCACCA ATTCCGGCTT GTCAACTTTT 9421 CCTGGAACCT CGTATGGGTT CTTGGCGAGG ATTGCGGTGT TGGTTGTGAG TGGATCGGAT 9481 TCTCCCTCCT TGTTCACAGC TCTGACACGG AATTTGTATT CGTGTCCGGG TTGAAGATTT 9541 GGAACTGTGG TCTTTGTTGT TTCACTTCTT CCACATGGGA CCCATCTTCC AGTTGCCAAA 9601 TCCATCTTTT CGATTTCATA ATGATCAATT GGAGCACCTC CGTCATCTTC TGGTACTTTC 9661 CACTCCAAAT CTGCGCGATC TTCGTAGACA TTCGACACTT CCAAAGGTCC CAATGGGCTA 9721 GATGGTTTTC CGAGCACAAT GACCTCAACG GAATGTTTGT CGCTTCCAGA AGCGTTGGTA 9781 GCAGTAAGAG TGTACAAACC AGCATGCTTG CGAGTTGCTC CACGGAGCAC AAATCGGGTC 9841 TTGTAGTCTT CGCTTTCAAT CTGAAATAAT CGTAAATTAG AATGTTCAAA AATATATAAA 9901 ATCTTACCCT GATCTTTTGA TCATCGACTG GTTTCTCATT GAAGATCCAT TCCTTCTTCG 9961 GTGGTGGTTC TCCGCGTACT GGAACATCGA ATTCCACATC GTTTCCTACC TTGATAGTGA 10021 TCGTCTTCAT TGCTTCACGA TCAATCCATG GCTTCAAGTT TCTTGGCTTT GCAACAACCT 10081 TTCGGCTTGG GTCTGAAGCC TCTCCCGGTC CGGCCTTGTT AACAGCCTTC ACACGGAACT 10141 GGTACTCTTC TCCCTCCTTG AGTCCAAGAA TCTCAGCATT GGTTTGATCT CCAGAAACCT 10201 TTCCGCATTC TTGCCAGTCT CTTCCATGTT TGCCCTTCTT CTCAATGACG TATTGAGTGA 10261 TTGGAGCTCC GCATCGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT 10321 CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT 10381 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC 10441 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA 10501 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT 10561 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA 10621 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT 10681 CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG 10741 TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT 10801 TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC 10861 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG 10921 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT 10981 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTAAAATTA AACATTTTTC 11041 TAAATTTTAT ATGATTTCTT TTAAATTTGC AAAAATTACT TAAATTTGAA TTCCCGCGCA 11101 AATGAGTGAC TTCATTTTCT GCATTATTGT GTTTTCCGGC TATATTAATA GGTATTTGTT 11161 TGTGTTTTTC TTTATTTTAT GATTCGAACT CCAATTTGTA AATTTTCGAA CATATTTCCC 11221 TAAAGAAAAA ATATGATTAA TCTGGAAAAA TTGGAAAATT ATTTTTCAAA TAAAAAACAA 11281 AGAAAAAAAT GAAGAAAAAC CTATTAGTTT GGCCATAAAA CGCAAAAATG TCGAAAATGA 11341 CGTCACTCAT CTGCGCGGGA AATCAAGAAT AATTCGGCCT TTTTTATTTT TTTGGAAAAT 11401 CGTAAAACAT TTAGAAAAAT TTTTTAATAG TTATAGTGGG ACTGTATTCT GTCATTTAGG 11461 GCAAAAGCCA GAGACGCTAC TCCACCGTTA ACATGAATTA TGAATATTAT TGCGACAAGA 11521 CCCAAACATT GATAAACCGC AAATCTAGAG ATCCCCGGGA ATTCGTAATC ATGGTCATAG 11581 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 11641 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 11701 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 11761 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG C // 1433 LOCUS ADDGENE_1433 5730 bp DNA circular SYN 01/03/2005 DEFINITION pPD16_43 complete plasmid sequence KEYWORDS . SOURCE pPD16_43 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1433 at www.addgene.org FEATURES Location/Qualifiers source 1..5730 /organism="Addgene plasmid pPD16_43" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 3644..3672 /label="Amp_promoter" gene 3714..4574 /label="Ampicillin" /gene="Ampicillin" CDS 3714..4574 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4729..5348 /label="pBR322_origin" misc_feature 5564..5590 /label="lacI.C" promoter 5657..5686 /label="lac_promoter" misc_feature 5721..5739 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 3301 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 3361 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 3421 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 3481 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT 3541 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 3601 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 3661 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 3721 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 3781 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 3841 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 3901 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 3961 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 4021 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 4081 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 4141 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 4201 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 4261 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 4321 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 4381 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 4441 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 4501 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 4561 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 4621 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 4681 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 4741 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 4801 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 4861 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 4921 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 4981 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 5041 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 5101 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG 5161 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 5221 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 5281 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 5341 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 5401 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 5461 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 5521 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 5581 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC 5641 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA 5701 GCGGATAACA ATTTCACACA GGAAACAGCT // 1434 LOCUS ADDGENE_1434 7003 bp DNA circular SYN 01/03/2005 DEFINITION pPD16_51 complete plasmid sequence KEYWORDS . SOURCE pPD16_51 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1434 at www.addgene.org FEATURES Location/Qualifiers source 1..7003 /organism="Addgene plasmid pPD16_51" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 4917..4945 /label="Amp_promoter" CDS 4987..5847 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4987..5847 /label="Ampicillin" /gene="Ampicillin" rep_origin 6002..6621 /label="pBR322_origin" misc_feature 6837..6863 /label="lacI.C" promoter 6930..6959 /label="lac_promoter" misc_feature 6994..7012 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 3301 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 3361 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 3421 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 3481 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 3541 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 3601 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 3661 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 3721 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 3781 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 3841 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 3901 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 3961 ATACATTCCC AATATACCAA ACATAACTGT TTAAAATTAA ACATTTTTCT AAATTTTATA 4021 TGATTTCTTT TAAATTTGCA AAAATTACTT AAATTTGAAT TCCCGCGCAA ATGAGTGACT 4081 TCATTTTCTG CATTATTGTG TTTTCCGGCT ATATTAATAG GTATTTGTTT GTGTTTTTCT 4141 TTATTTTATG ATTCGAACTC CAATTTGTAA ATTTTCGAAC ATATTTCCCT AAAGAAAAAA 4201 TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA GAAAAAAATG 4261 AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC GTCACTCATC 4321 TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC GTAAAACATT 4381 TAGAAAAATT TTTTAATAGT TATAGTGGGA CTGTATTCTG TCATTTAGGG CAAAAGCCAG 4441 AGACGCTACT CCACCGTTAA CATGAATTAT GAATATTATT GCGACAAGAC CCAAACATTG 4501 ATAAACCGCA AATCTAGCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG 4561 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT 4621 AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC 4681 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT 4741 GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC 4801 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT 4861 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 4921 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG 4981 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 5041 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 5101 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 5161 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 5221 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 5281 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 5341 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 5401 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 5461 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 5521 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 5581 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 5641 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 5701 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 5761 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 5821 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG 5881 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 5941 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA 6001 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA 6061 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 6121 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG 6181 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 6241 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 6301 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 6361 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC 6421 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 6481 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 6541 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 6601 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT 6661 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG 6721 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA 6781 GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC 6841 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG 6901 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG 6961 TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1435 LOCUS ADDGENE_1435 7081 bp DNA circular SYN 01/03/2005 DEFINITION pPD18_32 complete plasmid sequence KEYWORDS . SOURCE pPD18_32 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1435 at www.addgene.org FEATURES Location/Qualifiers source 1..7081 /organism="Addgene plasmid pPD18_32" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 4995..5023 /label="Amp_promoter" CDS 5065..5925 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5065..5925 /label="Ampicillin" /gene="Ampicillin" rep_origin 6080..6699 /label="pBR322_origin" misc_feature 6915..6941 /label="lacI.C" promoter 7008..7037 /label="lac_promoter" misc_feature 7072..7090 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT 3361 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 3421 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 3481 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 3541 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 3601 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 3661 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 3721 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 3781 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 3841 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 3901 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 3961 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 4021 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT AAAATTAAAC 4081 ATTTTTCTAA ATTTTATATG ATTTCTTTTA AATTTGCAAA AATTACTTAA ATTTGAATTC 4141 CCGCGCAAAT GAGTGACTTC ATTTTCTGCA TTATTGTGTT TTCCGGCTAT ATTAATAGGT 4201 ATTTGTTTGT GTTTTTCTTT ATTTTATGAT TCGAACTCCA ATTTGTAAAT TTTCGAACAT 4261 ATTTCCCTAA AGAAAAAATA TGATTAATCT GGAAAAATTG GAAAATTATT TTTCAAATAA 4321 AAAACAAAGA AAAAAATGAA GAAAAACCTA TTAGTTTGGC CATAAAACGC AAAAATGTCG 4381 AAAATGACGT CACTCATCTG CGCGGGAAAT CAAGAATAAT TCGGCCTTTT TTATTTTTTT 4441 GGAAAATCGT AAAACATTTA GAAAAATTTT TTAATAGTTA TAGTGGGACT GTATTCTGTC 4501 ATTTAGGGCA AAAGCCAGAG ACGCTACTCC ACCGTTAACA TGAATTATGA ATATTATTGC 4561 GACAAGACCC AAACATTGAT AAACCGCAAA TCTAGCCTAC TAGTCGGCCG TACGGGCCCT 4621 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 4681 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 4741 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 4801 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 4861 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 4921 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 4981 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 5041 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 5101 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 5161 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 5221 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 5281 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 5341 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 5401 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 5461 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 5521 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 5581 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 5641 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 5701 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 5761 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 5821 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 5881 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 5941 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 6001 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 6061 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 6121 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 6181 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 6241 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 6301 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 6361 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 6421 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 6481 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 6541 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 6601 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6661 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 6721 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 6781 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 6841 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 6901 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 6961 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 7021 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 7081 T // 1436 LOCUS ADDGENE_1436 10734 bp DNA circular SYN 01/03/2005 DEFINITION pPD18_49 complete plasmid sequence KEYWORDS . SOURCE pPD18_49 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1436 at www.addgene.org FEATURES Location/Qualifiers source 1..10734 /organism="Addgene plasmid pPD18_49" /mol_type="other DNA" misc_feature 35..58 /label="myo3.-2341" misc_feature 2354..2377 /label="myo3.Tsa" misc_feature 2420..2443 /label="myo3.N" CDS 3726..6971 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MNPPKFDKTEDMANLTFLNEGVPSSEKMTAPKKKRKVPVGEDQK QHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTN VTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVG YGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKP TTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGG EIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFRE VRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHY PNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNH PSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDE DQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGF VWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQ QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC FDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSH RHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 3807..3833 /label="SV40.NLS" gene 3924..6968 /label="lacZ" /gene="lacZ" promoter 8648..8676 /label="Amp_promoter" CDS 8718..9578 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8718..9578 /label="Ampicillin" /gene="Ampicillin" rep_origin 9733..10352 /label="pBR322_origin" misc_feature 10568..10594 /label="lacI.C" promoter 10661..10690 /label="lac_promoter" misc_feature 10725..10743 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCAAGC TTGGGCTGCA GGTCGGCTAT AATAAGTTCT TGAATAAAAT 61 AATTTTCCCG ACAAAACATG AGTATTTCTT TCGAAAATAA AAGTGCAGGC TAATTAGAGA 121 TTATTCTGTA ATTAACTGCA TAATTTGTCA CGTGCCATAG TTTTACATTC CACTACGTCA 181 TAGTTCTTAA AATACTAATC TCCTGAAAAT AGAAGTAGGT GAAGAAAGTT TAATTATCAG 241 TTCTAAAATG ACAATTGATC TTTGGAATAT GTTCTGAAAC TACCGATCAT TGAACAGATG 301 CTATTTGAAT GATATAGAAT TGTATATTTG CAATTTCTGA AACGCGTTCT TAAAGGCACA 361 CAGATTAATT CAAAAGGGTC TGGCCGCAAA AAGGTTTATG GTGGCCGATT TTGAGTTTTG 421 TGTGTGATTG CTTTTTCACA ATCAGTGTTT TCAGGATTAT GTGATGAACT AGATCTTCAA 481 GTTTCGTTAC ATTTCATATG TTTTCGGAAC TCACGAAGTA CATATTGGGT ATTGTGCTCA 541 AAAAATTCAG CAATCAGCTT CGCTCCGCTG ACTTTAGAAC CCAAAAAAAT AGTATGGCCA 601 AACTGACTGT GTTACGATCA TTTCAATTTT TCAATACATA TTTAAGATTT CTAAGAGTAA 661 GAAGGTCAAA AACTGTTCTG GAATACATAT ATATTTTTCA GGTTACAATT AGTCAAAAAG 721 TGCACTGAAA TATACGTTTT AATTTCACGA ATAACCCAAT TAGTTCAATG TATTTTTGGT 781 CAACCAACGT TAAAGTTTGG CTTCCAACCA ATTATCATTT CTGATCAACC ACAATGTTTT 841 TTCTTTATCT GCAAGTTAAT TTTTTATTTT TATCCAGATG TTTGGCATAT TTTTCAATTC 901 TTCACTAGCG CCCACTTCTT GCACTTCCGG CGCCCTGAAT CTAATGCATC TGTTGCAAGA 961 ATTGAAAGAC CAATCAACAC ATTGTTTTCT TCACGAGATA CTGAAGAAAA TGAATAAAAA 1021 CAGAGAAAAA GAGCCATGTG ATTAGTGACA ACTGTTGCTA ACAGATACCA TAGCTTGGAC 1081 TTGGTACGTG ATGGCAACGT ATGGGTCAAC AAAAATGATT GCAGAGGGGG TGCAAAACAG 1141 TCAAGTCGAG AAAATATGAA AAACAGAAAA CAAAGAACAG AAAAATGGGT TTGAGAGTCA 1201 GTATAATTTA TAAAAGAAAA ATTGTACATA GAAATTAACC ATTTTTGTAG AAGAAGTTAT 1261 TTTTCAAGCA TCGTTAAAAA TTATTCAAAG CACCTTATTT CATATTTAAT TTTAAACATG 1321 GTTAAATGAA CAACACGGTG CGCAATCAGG AAAACTTGAA ATCTGAAACT GTTGTTGTGA 1381 TCTTCTTCGC AACTGTTCAG ATAGCACTAG TGTAATGTTA AGAGTGCGCG AATATAATGG 1441 AATATAATGG ATCACACCTC CTGCCATCAG GTAAACGTCT CTGTTATCAC ATATTTCCAA 1501 CTATTAAATT TTTACCTTTT ACAGTTTTAC ATTTTTTTGA AAAAAGTAAC TTTTTGTCTT 1561 CAAAATCCCT GACGAAAATA TCAAATATTT TAATCGAGAC TGCAGAGGAA CCGATTGATG 1621 ATTTGGAAAA TCCAGCTTTA CCTGTGTAAG AACTGAAAAG TTTCATAACC CTAGGGTATT 1681 CCCAGTTACA TTCCCCACTG GCTAACAATA GCACCCAGTT TTTCATCACC TTTCTTCAAA 1741 TTTCTCGGCG ATTTGTTAAA AACAAAATTT GTGTCCCTTC TCTGATATCT CTATGTCTCT 1801 AAACACAAGT TCATCGGAAA ACGAAGGAGG GTAGGTGTTG GTTGGGCTCC CGAAGTGAAA 1861 ATAGAAGAGC AAGAATAGAA TATTAGAGAG AGAGTGCAGA GAGGGCGGGA TAGCTCCCGG 1921 GATTCCGTTT TCTTCTTCTT TATCTTCAAC GATGATGTGT GTGCGTGTTG TATAGATTCT 1981 GTTGCTCCCC CACAACTCGC TCCGAAGGCT CAATACAATT CAATTGATAT TGGAGGAGAG 2041 CCTACCGGAG TGGGAGGATA AGAAGAAACA TAAGAAGAAG AAGAAGAAGA AGCATGCTTC 2101 TGGTTTTTGA TGCTATGAAA ACGGCACAAA AAGATGATTG AGGTCCCTTT TCAATACCTT 2161 CTCTCATCTT TCAAATCCCA TTGAAACCTA AAACTTCTCA CCACGCTTTA CCATTGTTCT 2221 CCAAAAACTT ATAGCAATGT CTATAACTTT TTTATCTCTG AAAAGCAGTG TTCCATTTTT 2281 CTTTTTCCTA TTTTATTTCA ATTGTTTCTC ACATTTCGTT TGGATTCTTT GCTTGTCAAC 2341 CAGCTTCTTC TTCCACTTTT ACCGTCTAAT TTTCAGGGCA GGGAGCCATC AAACCCACGA 2401 CCACTAGATC CATCTAGAAA TGTCTGGAAA TCCAGACGCA TTCGAAAATG ATCCAGGATT 2461 CCCATTCTTG GGAATCTCGA GAGAGGCCAG AGCTGCCACT GCTGCTCGTC CTTTTGATTC 2521 CAAGAAGAAC TGCTGGATTC CGGATCCAGA AGATGGTAAG TCTTGATTTC GAATTGAAAA 2581 TTTTAAAGAA AAATTTTGAA CCACGTGCTG TAATATTTGG CAAGAATATT TTCCCATAAT 2641 TTTAATAGTA ACTGTGATAT GTTCTAATTT TAGAAATCAG TTGTCAACGC TAAAAATGTA 2701 AGGGTTACAA TAGTGGTATT GCAAAGAGTT ATGATGGTTA AGTTTGTAAA TACTTTTTTT 2761 CTGTTAATGA AACTAATTTG GATACAAATT TTTCATTTGA TCTAAACAGA ACATCTTAAT 2821 CAAAAAACAC TTTAAAACTC TTGCTTTGAT ATTGTTTGTT CAATTTCCTA ATTCTTACCA 2881 AACCAATTCA AGCTAACTTT CATTATGTCC ATTCCATTAA CAAAAACTAT TATTACACCT 2941 TCTCTCTCAT CGCCAGTGTC CACATTCATC CTACTCTCCT ACGCTTTCAT CTGTGTCTCT 3001 CAGATCTGCA TTTTATCTAT GATACATCAC ACTTTCCACC GACCCCGCCG GGCAACCAGT 3061 GGTGGCGGAT TTTTGAACAT CTGAGGTTCT CTCATCTCTC GGTCATCTGA TGAAGCACAT 3121 ACACACATAC ACACAACGGC ACTTTGTAAT CTTTCCGGAG AAGCGGGCAG GCACACACGT 3181 ACGCTATGAA GCCGGAGCAA GAGCTTCTCC TGCCGTTTGA ACTGCGGCTT CTGCTGTTGG 3241 CAGGAAAGAT GATGAGATTT CTTGGAATTT AAGATTGAGA TATTTGACGT CCCACATTTT 3301 ATGGATGGAC AACAATATGT ACATGTTTTG AAGATTTAAT ATTGAAATGT GGATATGAAA 3361 TCGGAATTTA AAAAGTCAAG GTCAATAAAA TGGTAATTCT GACCAGATCT ATTATTCGAG 3421 GATGACAAAT AAAACTAGGA TTAAATCAAA AATACTAAAT TTGAAAATGA AAAAGAAAAG 3481 ATTGTTCTAT AACTTATAAA TCTCACAATA ATGATTTTTG TACAGAAACA AAAACTAAAT 3541 GACATAATCG AATAAAACAT TCTGATTTTA ATCAGCCGCA TGCGACAAAA CATATATTTC 3601 TTGGAGGCTA ATATTAAATT TCAGGATTCG TCGCCGCCGA GATCCAATCC ACCACCGGCG 3661 AACAGGTGAC CGTGGTAACT GTGAAGGGAA ACCAGATTAC CGTCAAAAAA GATCAATGCC 3721 AAGAGATGAA CCCGCCAAAA TTCGACAAGA CCGAGGATAT GGCTAACTTG ACCTTTTTGA 3781 ATGAGGGGGT ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 3841 TGGGTGAAGA CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG 3901 CGCTGTATGG CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 3961 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 4021 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG 4081 CCTGGTTTCC GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG 4141 CCGATACTGT CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA 4201 CCAACGTGAC CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG 4261 GTTGTTACTC GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA 4321 TTATTTTTGA TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT 4381 ACGGCCAGGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG 4441 AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG 4501 ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC 4561 AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG 4621 AGGCTGAAGT TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT 4681 GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG 4741 AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT 4801 GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA 4861 CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC 4921 TGCTGCTGCT GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC 4981 CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC 5041 AGAACAACTT TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC 5101 TGTGCGACCG CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG 5161 TGCCAATGAA TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA 5221 CGCGAATGGT GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG 5281 AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT 5341 CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC 5401 CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA 5461 AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG 5521 CGATGGGTAA CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC 5581 GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA 5641 ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT 5701 TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA 5761 AACACCAGCA GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG 5821 AATACCTGTT CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA 5881 AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG 5941 AACTGCCTGA ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG 6001 TGCAACCGAA CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC 6061 GTCTGGCGGA AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA 6121 CCACCAGCGA AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC 6181 AGTCAGGCTT TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC 6241 GCGATCAGTT CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA 6301 TTGACCCTAA CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG 6361 CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG 6421 CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA 6481 GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC 6541 GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG 6601 GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC 6661 CATTGTCAGA CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA 6721 CGCGCGAATT GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC 6781 GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG 6841 GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC 6901 CGTCAGTATC GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 6961 GTCAAAAATA ATAGGGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT 7021 CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT 7081 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC 7141 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA 7201 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT 7261 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA 7321 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT 7381 CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG 7441 TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT 7501 TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC 7561 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG 7621 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT 7681 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTAAAATTA AACATTTTTC 7741 TAAATTTTAT ATGATTTCTT TTAAATTTGC AAAAATTACT TAAATTTGAA TTCCCGCGCA 7801 AATGAGTGAC TTCATTTTCT GCATTATTGT GTTTTCCGGC TATATTAATA GGTATTTGTT 7861 TGTGTTTTTC TTTATTTTAT GATTCGAACT CCAATTTGTA AATTTTCGAA CATATTTCCC 7921 TAAAGAAAAA ATATGATTAA TCTGGAAAAA TTGGAAAATT ATTTTTCAAA TAAAAAACAA 7981 AGAAAAAAAT GAAGAAAAAC CTATTAGTTT GGCCATAAAA CGCAAAAATG TCGAAAATGA 8041 CGTCACTCAT CTGCGCGGGA AATCAAGAAT AATTCGGCCT TTTTTATTTT TTTGGAAAAT 8101 CGTAAAACAT TTAGAAAAAT TTTTTAATAG TTATAGTGGG ACTGTATTCT GTCATTTAGG 8161 GCAAAAGCCA GAGACGCTAC TCCACCGTTA ACATGAATTA TGAATATTAT TGCGACAAGA 8221 CCCAAACATT GATAAACCGC AAATCTAGCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT 8281 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC 8341 AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 8401 TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 8461 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC 8521 TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT 8581 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 8641 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 8701 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 8761 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 8821 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 8881 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 8941 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 9001 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 9061 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 9121 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 9181 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 9241 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 9301 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 9361 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 9421 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 9481 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 9541 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 9601 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 9661 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 9721 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 9781 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 9841 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 9901 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 9961 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 10021 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 10081 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 10141 ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 10201 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 10261 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 10321 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 10381 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA 10441 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 10501 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 10561 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 10621 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 10681 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1437 LOCUS ADDGENE_1437 8255 bp DNA circular SYN 01/03/2005 DEFINITION pPD20_97 complete plasmid sequence KEYWORDS . SOURCE pPD20_97 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1437 at www.addgene.org FEATURES Location/Qualifiers source 1..8255 /organism="Addgene plasmid pPD20_97" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" CDS 1049..4498 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFA SWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQM HGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAF HLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGI FRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQ VASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIE AEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQN NFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERV TRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDI ICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAF RQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPH PALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDV APQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTL PAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAP LDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQG KTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQE NYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRY SQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVW CQK" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1334..1360 /label="SV40.NLS" gene 1451..4495 /label="lacZ" /gene="lacZ" promoter 6169..6197 /label="Amp_promoter" CDS 6239..7099 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6239..7099 /label="Ampicillin" /gene="Ampicillin" rep_origin 7254..7873 /label="pBR322_origin" misc_feature 8089..8115 /label="lacI.C" promoter 8182..8211 /label="lac_promoter" misc_feature 8246..8264 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGGT 1261 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 1321 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTGG GTGAAGACCA 1381 GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT TTCAACGCGC TGTATGGCGA 1441 GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA 1501 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA 1561 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC 1621 ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT 1681 CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA 1741 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT 1801 CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG 1861 CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGGACAG 1921 TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC 1981 GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT 2041 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT 2101 CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT GTACTGGAGG CTGAAGTTCA 2161 GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC 2221 GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA 2281 TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT 2341 CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC 2401 AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA 2461 CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA 2521 GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA 2581 CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA 2641 CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC GGCATGGTGC CAATGAATCG 2701 TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA CGCGTAACGC GAATGGTGCA 2761 GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG GGGAATGAAT CAGGCCACGG 2821 CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA 2881 GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG 2941 CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG TCCATCAAAA AATGGCTTTC 3001 GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC GCCCACGCGA TGGGTAACAG 3061 TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG 3121 CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG 3181 GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG 3241 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG GAAGCAAAAC ACCAGCAGCA 3301 GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG 3361 TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG 3421 CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT 3481 ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC 3541 GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG CAGTGGCGTC TGGCGGAAAA 3601 CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT 3661 GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT 3721 TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC 3781 CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC 3841 CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG 3901 CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA 3961 GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC 4021 GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA 4081 CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA 4141 CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT 4201 GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC TGCGGGACGC GCGAATTGAA 4261 TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC ATCAGCCGCT ACAGTCAACA 4321 GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA 4381 TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC 4441 GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA 4501 GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 4561 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 4621 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 4681 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 4741 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 4801 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 4861 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 4921 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 4981 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 5041 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 5101 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 5161 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 5221 ACATTCCCAA TATACCAAAC ATAACTGTTT AAAATTAAAC ATTTTTCTAA ATTTTATATG 5281 ATTTCTTTTA AATTTGCAAA AATTACTTAA ATTTGAATTC CCGCGCAAAT GAGTGACTTC 5341 ATTTTCTGCA TTATTGTGTT TTCCGGCTAT ATTAATAGGT ATTTGTTTGT GTTTTTCTTT 5401 ATTTTATGAT TCGAACTCCA ATTTGTAAAT TTTCGAACAT ATTTCCCTAA AGAAAAAATA 5461 TGATTAATCT GGAAAAATTG GAAAATTATT TTTCAAATAA AAAACAAAGA AAAAAATGAA 5521 GAAAAACCTA TTAGTTTGGC CATAAAACGC AAAAATGTCG AAAATGACGT CACTCATCTG 5581 CGCGGGAAAT CAAGAATAAT TCGGCCTTTT TTATTTTTTT GGAAAATCGT AAAACATTTA 5641 GAAAAATTTT TTAATAGTTA TATGGGACTT ATTCTGCATT TAGGGCAAAA GCCAGAGACT 5701 ACTCCACCGT TAACATGAAT TATGAATATT ATTGCGACAA GACCCAAACA TTGATAAACC 5761 CAAATCTAGC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 5821 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 5881 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 5941 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 6001 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 6061 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 6121 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 6181 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 6241 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 6301 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 6361 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 6421 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 6481 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 6541 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 6601 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 6661 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 6721 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 6781 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 6841 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 6901 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 6961 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 7021 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 7081 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 7141 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 7201 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 7261 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 7321 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 7381 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 7441 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 7501 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 7561 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 7621 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 7681 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 7741 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 7801 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 7861 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 7921 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 7981 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 8041 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 8101 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 8161 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 8221 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1438 LOCUS ADDGENE_1438 6556 bp DNA circular SYN 01/03/2005 DEFINITION pPD21_28 complete plasmid sequence KEYWORDS . SOURCE pPD21_28 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1438 at www.addgene.org FEATURES Location/Qualifiers source 1..6556 /organism="Addgene plasmid pPD21_28" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 4470..4498 /label="Amp_promoter" gene 4540..5400 /label="Ampicillin" /gene="Ampicillin" CDS 4540..5400 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5555..6174 /label="pBR322_origin" misc_feature 6390..6416 /label="lacI.C" promoter 6483..6512 /label="lac_promoter" misc_feature 6547..6565 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT 3361 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 3421 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 3481 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 3541 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 3601 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 3661 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 3721 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 3781 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 3841 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 3901 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 3961 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 4021 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC 4081 GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 4141 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 4201 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 4261 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 4321 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 4381 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 4441 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 4501 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 4561 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 4621 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 4681 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 4741 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 4801 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 4861 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 4921 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 4981 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 5041 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 5101 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 5161 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 5221 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 5281 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 5341 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 5401 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 5461 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 5521 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 5581 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 5641 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 5701 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 5761 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 5821 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 5881 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 5941 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 6001 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 6061 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 6121 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6181 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 6241 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 6301 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 6361 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 6421 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 6481 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 6541 CACACAGGAA ACAGCT // 1439 LOCUS ADDGENE_1439 6555 bp DNA circular SYN 01/03/2005 DEFINITION pPD22_04 complete plasmid sequence KEYWORDS . SOURCE pPD22_04 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1439 at www.addgene.org FEATURES Location/Qualifiers source 1..6555 /organism="Addgene plasmid pPD22_04" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..138 /label="rf22.04" misc_feature 153..179 /label="SV40.NLS" CDS 153..3317 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 270..3314 /label="lacZ" /gene="lacZ" promoter 4469..4497 /label="Amp_promoter" gene 4539..5399 /label="Ampicillin" /gene="Ampicillin" CDS 4539..5399 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5554..6173 /label="pBR322_origin" misc_feature 6389..6415 /label="lacI.C" promoter 6482..6511 /label="lac_promoter" misc_feature 6546..6564 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GAGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG 181 TACCGGTGGG TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT 241 TCAACGCGCT GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 301 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 361 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC 421 GCTTTGCCTG GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC 481 CTGAGGCCGA TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA 541 TCTACACCAA CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC 601 CGACGGGTTG TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA 661 CGCGAATTAT TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG 721 TCGGTTACGG CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG 781 CCGGAGAAAA CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG 841 ATCAGGATAT GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA 901 CTACACAAAT CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG 961 TACTGGAGGC TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT 1021 CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA 1081 TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA 1141 AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG 1201 ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA 1261 ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC 1321 ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA 1381 TGAAGCAGAA CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT 1441 ACACGCTGTG CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG 1501 GCATGGTGCC AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC 1561 GCGTAACGCG AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG 1621 GGAATGAATC AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG 1681 ATCCTTCCCG CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA 1741 TTTGCCCGAT GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT 1801 CCATCAAAAA ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG 1861 CCCACGCGAT GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT 1921 ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG 1981 ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC 2041 GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG 2101 AAGCAAAACA CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA 2161 CCAGCGAATA CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG 2221 ATGGTAAGCC GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT 2281 TGATTGAACT GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC 2341 GCGTAGTGCA ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC 2401 AGTGGCGTCT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC 2461 ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA 2521 ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC 2581 CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA 2641 CCCGCATTGA CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG 2701 AAGCAGCGTT GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG 2761 CTCACGCGTG GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG 2821 ATGGTAGTGG TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC 2881 CGGCGCGGAT TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG 2941 GATTAGGGCC GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG 3001 ATCTGCCATT GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT 3061 GCGGGACGCG CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA 3121 TCAGCCGCTA CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG 3181 AAGAAGGCAC ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT 3241 GGAGCCCGTC AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG 3301 TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC 3361 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 3421 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 3481 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 3541 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 3601 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 3661 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 3721 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 3781 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 3841 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 3901 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 3961 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4021 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4081 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4141 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4201 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 4261 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 4321 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 4381 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 4441 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 4501 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 4561 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 4621 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 4681 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 4741 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 4801 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 4861 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 4921 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 4981 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5041 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5101 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5161 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 5221 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 5281 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 5341 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 5401 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 5461 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 5521 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 5581 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 5641 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 5701 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 5761 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 5821 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 5881 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 5941 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6001 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6061 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6121 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6181 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 6241 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 6301 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 6361 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 6421 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 6481 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 6541 ACACAGGAAA CAGCT // 1440 LOCUS ADDGENE_1440 6560 bp DNA circular SYN 01/03/2005 DEFINITION pPD22_11 complete plasmid sequence KEYWORDS . SOURCE pPD22_11 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1440 at www.addgene.org FEATURES Location/Qualifiers source 1..6560 /organism="Addgene plasmid pPD22_11" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..139 /label="rf22.11" misc_feature 158..184 /label="SV40.NLS" CDS 158..3322 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 275..3319 /label="lacZ" /gene="lacZ" promoter 4474..4502 /label="Amp_promoter" CDS 4544..5404 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4544..5404 /label="Ampicillin" /gene="Ampicillin" rep_origin 5559..6178 /label="pBR322_origin" misc_feature 6394..6420 /label="lacI.C" promoter 6487..6516 /label="lac_promoter" misc_feature 6551..6569 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 181 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 241 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 301 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 361 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 421 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 481 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 541 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 601 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 661 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 721 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 781 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 841 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 901 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 961 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1021 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1081 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1141 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1201 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1261 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1321 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT 1381 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1441 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1501 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1561 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1621 GCTGGGGAAT GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC 1681 TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA 1741 TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA 1801 ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA 1861 ATACGCCCAC GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 1921 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 1981 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2041 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT 2101 GACGGAAGCA AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA 2161 AGTGACCAGC GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC 2221 GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA 2281 ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC 2341 AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG 2401 GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT 2461 CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA 2521 ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT 2581 GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA 2641 AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA 2701 GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC 2761 GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG 2821 GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC 2881 GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG 2941 GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG 3001 CTGGGATCTG CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 3061 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 3121 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 3181 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 3241 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3301 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GATCGCCATC TCGCGCCCGT 3361 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 3421 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 3481 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 3541 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 3601 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 3661 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 3721 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 3781 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 3841 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 3901 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 3961 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 4021 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT 4081 AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 4141 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 4201 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA 4261 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 4321 AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 4381 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 4441 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 4501 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 4561 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4621 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 4681 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 4741 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 4801 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 4861 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 4921 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 4981 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 5041 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 5101 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 5161 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5221 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 5281 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 5341 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 5401 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 5461 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 5521 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 5581 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 5641 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 5701 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 5761 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 5821 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 5881 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 5941 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 6001 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 6061 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 6121 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 6181 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 6241 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 6301 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 6361 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 6421 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 6481 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 6541 ATTTCACACA GGAAACAGCT // 1441 LOCUS ADDGENE_1441 7242 bp DNA circular SYN 01/03/2005 DEFINITION pPD26_77 complete plasmid sequence KEYWORDS . SOURCE pPD26_77 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1441 at www.addgene.org FEATURES Location/Qualifiers source 1..7242 /organism="Addgene plasmid pPD26_77" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" misc_feature 3921..3944 /label="glp1.3'" promoter complement(4528..4547) /label="T3_promoter" misc_feature complement(4564..4582) /label="M13r_primer" misc_feature complement(4713..4739) /label="lacI.C" promoter 5156..5184 /label="Amp_promoter" CDS 5226..6086 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5226..6086 /label="Ampicillin" /gene="Ampicillin" rep_origin 6241..6860 /label="pBR322_origin" misc_feature 7076..7102 /label="lacI.C" promoter 7169..7198 /label="lac_promoter" misc_feature 7233..7251 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCGAAAC AAATCATCTG ACACCACCAC CGTCTGATGG ATCGTTCTCA TCTCCGTCTC 3301 CACATTATTA TCCGACGACT ACATCGACAC CGAATCGAAT GGAAACAAGT CCGGAGTACA 3361 TGTTTAACCA TGAAATGGTG GGTAGATGAT TATTAAAATG TTTAAGAAAA TTAAATAATT 3421 TGTTTTAGGC ACCACCGGTC AATGCGATGT GGTATACTAC ACCACCTCCT TATCAAGATC 3481 CAAACTATCG TCATGTGCCT CCAAATACTG CATTTCAAAA TGCAGAGCAA ATGAATGGCT 3541 CCTTCTACTG TTAATCTATT TAATTCATTA ATTTTTCATT TATTGACTGT ATCCCGGATG 3601 TTTCTTGTCC TCCCAACATA TCTCCTAACT GCTCGGTTCA TTTTAAATAT GCTCATCTCA 3661 CTACATCACC CAGACACTGG TCCCCACAGA GTTTTTTGTA TACTATTTCG GGTCATTTTT 3721 CTTATTCTAG ACTAATATTG TAAGCTATAA GTTGTAGAAT AATTATTGAT CCAAATCAGA 3781 TTAAGAGTAT AAGCTTTGTT TTTTCTCCTT TTCTTTATAA CTTGTTACAA TTTTTGAAAT 3841 TCCCTTTTTT GACAGGCTTT TATTACACTG TAACTGTGTT TCTTATCTTG CAAACATTTA 3901 ATGAATTGTA ATTCTTTAGT ATCTTGAGGG CTTTTTGTTT TTCGAATTAT TGAAGCTCAA 3961 AGTTCCAGTT TTACTACGAT CCAGCGAATT CTCCTCATTT CGATCCGATG CAATTGACTT 4021 CAGATCAATA TTGGTTGCCT GAAAGAAATA ATTGTGAGCA TTTTTGTCAA AAAACAGAGA 4081 ACTCACCATT CTCGAGGCTC CCGTTCCAGG AGCAGTACTT GGTGATGGAC ACGTAGATTG 4141 ATTAAACCAA ACCAAAGGTT CTTTCAGAGT CAACTTACAG CCTCGAGCGT AGTCCGTGAT 4201 AGCTTCTCGC AGAACACTGA AAATTGGAAA TTTATTGGAA TAAAAACTTT TTCTGCACTT 4261 TATAGAATAA AAAAATCATG AATTTACCCG AATTTAACCT CCGAATCGTA GACCAAATTG 4321 TCCAAGTAGA TGGAAATCAC CTTGAACATC GGATGTTTTT CATATGCTGA AAATAAATTA 4381 ATGAATTTAT GTAATTTTTT AAATAATTAC TTTTCAATTT GGTGAACAAT TCCTGCTTCT 4441 TTGCATAGGC ATCTGGACGA GTGAGTCCTT TCCAATCAAT CAATGTGGTG TCGACCTCGA 4501 GGGGGGGCCC GGTACCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTCCG AGCTTGGCGT 4561 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 4621 TAGGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGG TAACTCACAT 4681 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 4741 AATGAATCGG CCAACGCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG 4801 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 4861 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 4921 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG 4981 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG 5041 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG 5101 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 5161 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 5221 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 5281 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 5341 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 5401 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 5461 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 5521 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 5581 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 5641 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 5701 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 5761 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 5821 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 5881 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 5941 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 6001 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 6061 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 6121 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 6181 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 6241 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 6301 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 6361 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 6421 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 6481 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 6541 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 6601 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG 6661 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 6721 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 6781 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 6841 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 6901 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT 6961 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG 7021 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA 7081 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA 7141 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT 7201 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1442 LOCUS ADDGENE_1442 4427 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_35 complete plasmid sequence KEYWORDS . SOURCE pPD30_35 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1442 at www.addgene.org FEATURES Location/Qualifiers source 1..4427 /organism="Addgene plasmid pPD30_35" /mol_type="other DNA" misc_feature 583..606 /label="unc54.5'" misc_feature 624..665 /label="SynIVS.A" misc_feature 672..689 /label="rf21.28" promoter 2341..2369 /label="Amp_promoter" gene 2411..3271 /label="Ampicillin" /gene="Ampicillin" CDS 2411..3271 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3426..4045 /label="pBR322_origin" misc_feature 4261..4287 /label="lacI.C" promoter 4354..4383 /label="lac_promoter" misc_feature 4418..4436 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGTGAGT ATTTTCGGTT TTTCACTGGT 61 TCTCTGTAAA AAGAGTAATG CAAAGGGCAA GTTAACTTAG GTCGTAAATG TATTGAATTT 121 GCTTAAAATC TGAAGATCTA GTGGTGAACC GTGGAAGATT ATCAAGAGGA GGCTGAAGAT 181 CTGTTTAAGA ACCATTAATC AAACTGGTAT TCTATTTTCA CTGGTTGTAT GTAAACATTC 241 TATCTTATTC CTTTTATCAC TGTTCTGCAC TTTCCTATAA AAAAAGTTGA CCGACCGTAC 301 TCTCTGAATT CATTTTTCCC GATCTTACCA ACTCCCGATC TATCTCTATC CCTGGTTTTT 361 TCTTCGTGCT CCAATGGAAT TCTTGAGACT TCCACTATCT TCTCTGGCAC CCTCCACTAC 421 GCGTAGGCGT CTCTCGCTTC GTGTATTCCC GGGAAGCCGG GTTCCCGTCT CTCCCGCCGC 481 TGCCGCTGCC GCACACAGCT TTACACCTCG TAGAATCCCC AAAGAGGGGC GTGGCTTGCG 541 GGTGCCAACA TCCTCCTGCC GAGGAAGAAG CAGGCACTCA TCACTCGCAT CATCAACCTC 601 GGGATTGGCC AAAGGACCCA AAGGTATGTT TCGAATGATA CTAACATAAC ATAGAACATT 661 TTCAGGAGGA CCCTTGGCTA GCGTCGACGG TACCATGGTA TTGATATCTG AGCTCCGCAT 721 CGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC 781 TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT 841 CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA 901 AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC 961 CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT 1021 TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT 1081 ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG 1141 GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT 1201 TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC 1261 TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA 1321 GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG 1381 AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA 1441 CATTCCCAAT ATACCAAACA TAACTGTTTA AAATTAAACA TTTTTCTAAA TTTTATATGA 1501 TTTCTTTTAA ATTTGCAAAA ATTACTTAAA TTTGAATTCC CGCGCAAATG AGTGACTTCA 1561 TTTTCTGCAT TATTGTGTTT TCCGGCTATA TTAATAGGTA TTTGTTTGTG TTTTTCTTTA 1621 TTTTATGATT CGAACTCCAA TTTGTAAATT TTCGAACATA TTTCCCTAAA GAAAAAATAT 1681 GATTAATCTG GAAAAATTGG AAAATTATTT TTCAAATAAA AAACAAAGAA AAAAATGAAG 1741 AAAAACCTAT TAGTTTGGCC ATAAAACGCA AAAATGTCGA AAATGACGTC ACTCATCTGC 1801 GCGGGAAATC AAGAATAATT CGGCCTTTTT TATTTTTTTG GAAAATCGTA AAACATTTAG 1861 AAAAATTTTT TAATAGTTAT AGTGGGACTG TATTCTGTCA TTTAGGGCAA AAGCCAGAGA 1921 CGCTACTCCA CCGTTGGGGG ATCCACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT 1981 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 2041 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG 2101 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 2161 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG 2221 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC 2281 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 2341 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 2401 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT 2461 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 2521 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG 2581 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 2641 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 2701 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT 2761 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 2821 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT 2881 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 2941 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 3001 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC 3061 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 3121 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT 3181 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 3241 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT 3301 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 3361 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 3421 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3481 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3541 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 3601 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3661 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3721 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3781 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA 3841 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3901 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3961 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4021 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4081 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 4141 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 4201 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 4261 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 4321 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 4381 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1443 LOCUS ADDGENE_1443 4812 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_38 complete plasmid sequence KEYWORDS . SOURCE pPD30_38 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1443 at www.addgene.org FEATURES Location/Qualifiers source 1..4812 /organism="Addgene plasmid pPD30_38" /mol_type="other DNA" misc_feature 120..150 /label="unc54.enh" misc_feature 968..991 /label="unc54.5'" misc_feature 1009..1050 /label="SynIVS.A" misc_feature 1057..1074 /label="rf21.28" promoter 2726..2754 /label="Amp_promoter" CDS 2796..3656 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2796..3656 /label="Ampicillin" /gene="Ampicillin" rep_origin 3811..4430 /label="pBR322_origin" misc_feature 4646..4672 /label="lacI.C" promoter 4739..4768 /label="lac_promoter" misc_feature 4803..4821 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGTCTTCT TCTAAATTCC CATAAAATCC CGAAACTCCT 61 TCCCTCTATC TTCTTTTTCT TCTCGTTTTC AAATGTTTCT CTCTATCCCA TTCTCTCATC 121 AATTGAGTGG GATGAGGCTA TCTCTGCCTC TCTTCTGAAT CTCTGAACCA TCTTACATTA 181 CACTGTGGAT GACGAGCCCC ACAGGCTCCC TTGCATCAGA TACTGCCATT GGGGATGGCA 241 AAGAAGAGAG AAGGTATTGT GAGGATATAT TTTTCTAAGA AAAAACGTTT GAAGAAAAGA 301 AGATGAAGAA GATCTGCTTG ATTCATTGCA CAAGTTAGAA GTAACAGGGG TCTATATTTC 361 GAAGAACTTA AAGGGAATGC AACTGAACAT AAAATTAAAC AAAGGGATTG AATCCTGCAG 421 TGAGTATTTT CGGTTTTTCA CTGGTTCTCT GTAAAAAGAG TAATGCAAAG GGCAAGTTAA 481 CTTAGGTCGT AAATGTATTG AATTTGCTTA AAATCTGAAG ATCTAGTGGT GAACCGTGGA 541 AGATTATCAA GAGGAGGCTG AAGATCTGTT TAAGAACCAT TAATCAAACT GGTATTCTAT 601 TTTCACTGGT TGTATGTAAA CATTCTATCT TATTCCTTTT ATCACTGTTC TGCACTTTCC 661 TATAAAAAAA GTTGACCGAC CGTACTCTCT GAATTCATTT TTCCCGATCT TACCAACTCC 721 CGATCTATCT CTATCCCTGG TTTTTTCTTC GTGCTCCAAT GGAATTCTTG AGACTTCCAC 781 TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA TTCCCGGGAA 841 GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA CCTCGTAGAA 901 TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA AGAAGCAGGC 961 ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA 1021 TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGCTAGCGTC GACGGTACCA 1081 TGGTATTGAT ATCTGAGCTC CGCATCGGCC GCTGTCATCA GATCGCCATC TCGCGCCCGT 1141 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 1201 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 1261 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 1321 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 1381 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 1441 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 1501 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 1561 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 1621 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 1681 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 1741 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 1801 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTAAAATT 1861 AAACATTTTT CTAAATTTTA TATGATTTCT TTTAAATTTG CAAAAATTAC TTAAATTTGA 1921 ATTCCCGCGC AAATGAGTGA CTTCATTTTC TGCATTATTG TGTTTTCCGG CTATATTAAT 1981 AGGTATTTGT TTGTGTTTTT CTTTATTTTA TGATTCGAAC TCCAATTTGT AAATTTTCGA 2041 ACATATTTCC CTAAAGAAAA AATATGATTA ATCTGGAAAA ATTGGAAAAT TATTTTTCAA 2101 ATAAAAAACA AAGAAAAAAA TGAAGAAAAA CCTATTAGTT TGGCCATAAA ACGCAAAAAT 2161 GTCGAAAATG ACGTCACTCA TCTGCGCGGG AAATCAAGAA TAATTCGGCC TTTTTTATTT 2221 TTTTGGAAAA TCGTAAAACA TTTAGAAAAA TTTTTTAATA GTTATAGTGG GACTGTATTC 2281 TGTCATTTAG GGCAAAAGCC AGAGACGCTA CTCCACCGTT GGGGGATCCA CTAGTCGGCC 2341 GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 2401 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 2461 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA 2521 GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 2581 ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 2641 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 2701 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 2761 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 2821 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 2881 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 2941 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 3001 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 3061 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 3121 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 3181 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 3241 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 3301 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 3361 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 3421 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 3481 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 3541 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 3601 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 3661 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 3721 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 3781 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 3841 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 3901 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 3961 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 4021 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 4081 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 4141 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 4201 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 4261 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 4321 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 4381 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 4441 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 4501 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 4561 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 4621 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 4681 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 4741 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 4801 CAGGAAACAG CT // 1444 LOCUS ADDGENE_1444 5087 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_69 complete plasmid sequence KEYWORDS . SOURCE pPD30_69 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1444 at www.addgene.org FEATURES Location/Qualifiers source 1..5087 /organism="Addgene plasmid pPD30_69" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1307..1324 /label="rf21.28" promoter 3001..3029 /label="Amp_promoter" CDS 3071..3931 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3071..3931 /label="Ampicillin" /gene="Ampicillin" rep_origin 4086..4705 /label="pBR322_origin" misc_feature 4921..4947 /label="lacI.C" promoter 5014..5043 /label="lac_promoter" misc_feature 5078..5096 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGGT 1261 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGCTAGCGTC 1321 GACGGTACCA TGGTATTGAT ATCTGAGCTC CGCATCGGCC GCTGTCATCA GATCGCCATC 1381 TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT 1441 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG 1501 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT 1561 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA 1621 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT 1681 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT 1741 TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT 1801 CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA 1861 TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC 1921 GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA 1981 AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA 2041 GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT 2101 GTTTAAAATT AAACATTTTT CTAAATTTTA TATGATTTCT TTTAAATTTG CAAAAATTAC 2161 TTAAATTTGA ATTCCCGCGC AAATGAGTGA CTTCATTTTC TGCATTATTG TGTTTTCCGG 2221 CTATATTAAT AGGTATTTGT TTGTGTTTTT CTTTATTTTA TGATTCGAAC ATATTTCCCT 2281 AAAGAAAAAA TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA 2341 GAAAAAAATG AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC 2401 GTCACTCATC TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC 2461 GTAAAACATT TAGAAAAATT TTTTAATAGT TATATGGGAC TTATTCTGCA TTTAGGGCAA 2521 AAGCCAGAGA CTACTCCACC GTTAACATGA ATTATGAATA TTATTGCGAC AAGACCCAAA 2581 CATTGATAAA CCCAAATCTA GCCTACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT 2641 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 2701 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG 2761 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 2821 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG 2881 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC 2941 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 3001 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 3061 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT 3121 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 3181 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG 3241 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 3301 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 3361 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT 3421 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 3481 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT 3541 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 3601 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 3661 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC 3721 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 3781 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT 3841 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 3901 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT 3961 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 4021 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 4081 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 4141 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 4201 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 4261 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 4321 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 4381 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 4441 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA 4501 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 4561 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 4621 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4681 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4741 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 4801 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 4861 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 4921 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 4981 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 5041 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1445 LOCUS ADDGENE_1445 6613 bp DNA circular SYN 01/03/2005 DEFINITION pPD34_110 complete plasmid sequence KEYWORDS . SOURCE pPD34_110 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1445 at www.addgene.org FEATURES Location/Qualifiers source 1..6613 /organism="Addgene plasmid pPD34_110" /mol_type="other DNA" CDS 1..3375 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPRESWQRALVLLIVLLFIVIFVITVLFVIRSNKVPVGEDQKQHLELSRDIAQRFNA LYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR FAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTE NPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSA FLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFND DFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTL RLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPL LIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKW LSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDEN GNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVT SEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLT VRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKR WQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAA GHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNID GFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 160..225 /label="SynTmD" gene 328..3372 /label="lacZ" /gene="lacZ" promoter 4527..4555 /label="Amp_promoter" gene 4597..5457 /label="Ampicillin" /gene="Ampicillin" CDS 4597..5457 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5612..6231 /label="pBR322_origin" misc_feature 6447..6473 /label="lacI.C" promoter 6540..6569 /label="lac_promoter" misc_feature 6604..6622 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC TCGAGAAAGC TGGCAAAGGG CTCTTGTCCT GCTAATCGTA 181 CTACTCTTCA TCGTCATCTT CGTTATTACT GTTTTGTTCG TCATAAGATC TAACAAGGTA 241 CCGGTGGGTG AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC 301 AACGCGCTGT ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 361 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 421 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC 481 TTTGCCTGGT TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT 541 GAGGCCGATA CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC 601 TACACCAACG TGACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG 661 ACGGGTTGTT ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG 721 CGAATTATTT TTGATGGCGT TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC 781 GGTTACGGCC AGGACAGTCG TTTGCCGTCT GAATTTGACC TGAGCGCATT TTTACGCGCC 841 GGAGAAAACC GCCTCGCGGT GATGGTGCTG CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT 901 CAGGATATGT GGCGGATGAG CGGCATTTTC CGTGACGTCT CGTTGCTGCA TAAACCGACT 961 ACACAAATCA GCGATTTCCA TGTTGCCACT CGCTTTAATG ATGATTTCAG CCGCGCTGTA 1021 CTGGAGGCTG AAGTTCAGAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 1081 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 1141 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 1201 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 1261 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 1321 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 1381 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATAT CCTGCTGATG 1441 AAGCAGAACA ACTTTAACGC CGTGCGCTGT TCGCATTATC CGAACCATCC GCTGTGGTAC 1501 ACGCTGTGCG ACCGCTACGG CCTGTATGTG GTGGATGAAG CCAATATTGA AACCCACGGC 1561 ATGGTGCCAA TGAATCGTCT GACCGATGAT CCGCGCTGGC TACCGGCGAT GAGCGAACGC 1621 GTAACGCGAA TGGTGCAGCG CGATCGTAAT CACCCGAGTG TGATCATCTG GTCGCTGGGG 1681 AATGAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1741 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1801 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 1861 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATCCTTTG CGAATACGCC 1921 CACGCGATGG GTAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT 1981 CCCCGTTTAC AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT 2041 GAAAACGGCA ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC 2101 CAGTTCTGTA TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC GCTGACGGAA 2161 GCAAAACACC AGCAGCAGTT TTTCCAGTTC CGTTTATCCG GGCAAACCAT CGAAGTGACC 2221 AGCGAATACC TGTTCCGTCA TAGCGATAAC GAGCTCCTGC ACTGGATGGT GGCGCTGGAT 2281 GGTAAGCCGC TGGCAAGCGG TGAAGTGCCT CTGGATGTCG CTCCACAAGG TAAACAGTTG 2341 ATTGAACTGC CTGAACTACC GCAGCCGGAG AGCGCCGGGC AACTCTGGCT CACAGTACGC 2401 GTAGTGCAAC CGAACGCGAC CGCATGGTCA GAAGCCGGGC ACATCAGCGC CTGGCAGCAG 2461 TGGCGTCTGG CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT 2521 CTGACCACCA GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC 2581 CGCCAGTCAG GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG 2641 CTGCGCGATC AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC 2701 CGCATTGACC CTAACGCCTG GGTCGAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA 2761 GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT 2821 CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT 2881 GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG 2941 GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA 3001 TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT 3061 CTGCCATTGT CAGACATGTA TACCCCGTAC GTCTTCCCGA GCGAAAACGG TCTGCGCTGC 3121 GGGACGCGCG AATTGAATTA TGGCCCACAC CAGTGGCGCG GCGACTTCCA GTTCAACATC 3181 AGCCGCTACA GTCAACAGCA ACTGATGGAA ACCAGCCATC GCCATCTGCT GCACGCGGAA 3241 GAAGGCACAT GGCTGAATAT CGACGGTTTC CATATGGGGA TTGGTGGCGA CGACTCCTGG 3301 AGCCCGTCAG TATCGGCGGA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3361 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG 3421 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 3481 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 3541 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 3601 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 3661 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 3721 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 3781 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 3841 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 3901 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 3961 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 4021 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 4081 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 4141 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 4201 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 4261 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 4321 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 4381 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 4441 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 4501 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 4561 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 4621 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 4681 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 4741 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 4801 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 4861 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 4921 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 4981 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 5041 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 5101 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 5161 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 5221 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 5281 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 5341 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 5401 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 5461 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 5521 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 5581 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 5641 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 5701 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 5761 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 5821 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 5881 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 5941 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 6001 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 6061 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 6121 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 6181 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 6241 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 6301 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 6361 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 6421 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 6481 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 6541 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 6601 ACAGGAAACA GCT // 1446 LOCUS ADDGENE_1446 5156 bp DNA circular SYN 01/03/2005 DEFINITION pPD47_52 complete plasmid sequence KEYWORDS . SOURCE pPD47_52 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1446 at www.addgene.org FEATURES Location/Qualifiers source 1..5156 /organism="Addgene plasmid pPD47_52" /mol_type="other DNA" promoter 6..25 /label="Sp6_promoter" promoter complement(75..93) /label="T7_promoter" misc_feature 345..374 /label="Ori.Lam>>" CDS complement(446..1147) /label="Orf1" /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK IAEIKAKFGLKGASV" CDS complement(1144..2043) /label="Orf0" /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ AGVTASKPKLDLTNTDWIYGVDL" misc_feature 3238..3267 /label="Ori.Lam<<" promoter 3487..3536 /label="NeoKan_promoter" CDS 3624..4286 /label="Orf2" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVATAI RT" gene 3627..4581 /label="Neomycin(w/ gaps)" /gene="Neomycin(w/ gaps)" ORIGIN 1 ATTAgattta ggtgacacta tagaagcttg gatccacgtg tcgactctag agcggccgcg 61 tatacgggcc ctctccctat agtgagtcgt attaATTGTA GCCGCGTTCT AACGACAATA 121 TGTACAAGCC TAATTGTGTA GCATCTGGCT TACTGAAGCA GACCCTATCA TCTCTCTCGT 181 AAACTGCCGT CAGAGTCGGT TTGGTTGGAC GAACCTTCTG AGTTTCTGGT AACGCCGTCC 241 CGCACCCGGA AATGGTCAGC GAACCAATCA GCAGGGTCAT CGCTAGAAAT CATCCTTAGC 301 GAAAGCTAAG GATTTTTTTT ATCTGAATTC TAGCCAGATC CCCGCTGATT TATGCTGGTT 361 ACTGTTGCGC CTGTTAGCGC GGCAACGTCC GGCGCACAGA AGCTATTATG CGTCCCCAGG 421 TAATGAATAA TTGCCTCTTT GCCCGTCATA CACTTGCTCC TTTCAGTCCG AACTTAGCTT 481 TGATTTCTGC GATCTTCGCC AGAGCCTGTG CACGATTTAG AGGTCTACCG CCCATGACAG 541 GAAGTTGTTT TACTGGTTCA GGGATCGCCT CACCACGGTT AATTCTCGCA GTCATATGGA 601 CAAGCTCATC TGCGGCCTTA CGGCGTAATT CCGCATCAGT AAGCGCATTG GCCCGCATGT 661 TCTGATACAG GTTGGTAACC AGCCAGTAGT GCGCGTTTGA TTTCCACGGA TAAGACTCCG 721 CATCCGGATA CAGGCCTCGC TTCCGGCAAT ACTCGTAAAC CATATCAACC AGCTCGCTGA 781 CGTTTGGCAG TCCGGCGGTA ACGGATGCTT CTTCCCGGCA CCATGCAACA AACTGCCCGG 841 GTGATGGCAG AAATGGTCGA TTCTGCCGAC GGGCTACGCG CATTCCTGCG TTAACCTGTT 901 CCATCGTGGT GATCCCGTTT TCCCGAAAAG CCAGAACCCA CTGGCGACGG ATTTCGTTCA 961 CTTCGTTCTG GTCACGGTTA GCCAGGCTCG CCGGGAAAGT TGCCAGTAAC TGGCTGAACA 1021 CACCGTTGAT GATCTGCGCT ACCTGCTGTA CCTGCGGCTT TTCGTCGTAC TGTTCCGGCA 1081 TGTTGTTGGC GATCCGACGC ATCTGCTCAC GGTCAAAGTT AACCATCTGT GCGGCGATGT 1141 TTTTCATAGA TCCACCCCGT AAATCCAGTC TGTGTTTGTC AGGTCGAGTT TTGGTTTGCT 1201 GGCTGTCACG CCTGCCTGTT GCTTGTTACG GTTGATTTCG AGTTGGGTCC ACTTATCGCG 1261 GAGTTTGGCC GGGCTCAGCA CGTTACCGGA CCAGAAGTTG TCCTGGCATG CCCAGCGGAA 1321 CAGCACACAC ATGTCGCGGT GGTTACGTCC GTCACGTTCA CGCATCAGGC GGATATCGTT 1381 AGCCCACCCA GCAAAATTCG GTTTTCTGGC TGATGGTGCG ATAGTCTTCA CCATGTCAAA 1441 CATCCACTCT GCGGCGGTCA GGTCTTCTGC TGTCCCCCAC TTGCTGCCGC TCTGAATTGC 1501 AGCATCCGGT TTCACCACAG AAAGGTCGTT TTCTGGCTGG TCAGAGGATT CGCCAGAATT 1561 CTCTGACGAA TAATCTTTTC TTTTTTCTTT TGTAATAGTG TCTTTTGTGT CCCCCTGTTT 1621 TGAGGGATAG CAATCCCCCA ATTTGAGGGA TGTTTTATCC CTCGTTTTAG GGGATTTTCC 1681 CTCGTTTTGA GGGATGCACC ATTCTGAGAT GTTTTTATTT GGTCCAAACA TGCCGCCTTG 1741 CTGCTTGATA ATATTCATTC TGACGAGTTC TAACTTGGCT TCATTGCACC GTTTGACAGG 1801 TAACTTTGTA ATCTCGCTAA GTTGAGAATC GGTGATTCTG TCCATTGGTT TATTCCACCC 1861 ATAGGTTTTA CGCAGAATGG CAAGCAGCAC TTTAAACTGT CGCTTGGTCA GATCTGCGCC 1921 CGAATAAGCC TCAAGCAGCA TATTTGATAG TCTGGCGTAA CCATCATCGA GATCTGCCAC 1981 ATTACGCTCC TGTCCGGCAA AGTTACCTCT GCCGAAGTTG AGTATTTTTG CTGTATTTGT 2041 CATAATGACT CCTGTTGATA GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA 2101 GAACGCTCGG TTGCCGCCGG GCGTTTTTTA TTGGTGAGAA TCGCAGCAAC TTGTCGCGCC 2161 AATCGAGCCA TGTCGTCGTC AACGACCCCC CATTCAAGAA CAGCAAGCAG CATTGAGAAC 2221 TTTGGAATCC AGTCCCTCTT CCACCTGCTG ATCTGCGACT TATCAACGCC CACAGCTTCC 2281 GCTGTCTTCT CAGTTCCAAG CATTGCGATT TTGTTAAGCA ACGCACTCTC GATTCGTAGA 2341 GCCTCGTTGC GTTTGTTTGC ACGAACCATA TGTAAGTATT TCCTTAGATA ACAATTGATT 2401 GAATGTATGC AAATAAATGC ATACACCATA GGTGTGGTTT AATTTGATGC CCTTTTTCAG 2461 GGCTGGAATG TGTAAGAGCG GGGTTATTTA TGCTGTTGTT TTTTTGTTAC TCGGGAAGGG 2521 CTTTACCTCT TCCGCATAAA CGCTTCCATC AGCGTTTATA GTTAAAAAAA TCTTTCGGCC 2581 TGCATGAATG GCCTTGTTGA TCGCGCTTTG ATATACGCCG AGATCTTTAG CTGTCTTGGT 2641 TTGCCCAAAG CGCATTGCAT AATCTTTCAG GGTTATGCGT TGTTCCATAC AACCTCCTTA 2701 GTACATGCAA CCATTATCAC CGCCAGAGGT AAAATAGTCA ACACGCACGG TGTTAGATAT 2761 TTATCCCTTG CGGTGATAGA TTTAACGTAT GAGCACAAAA AAGAAACCAT TAACACAAGA 2821 GCAGCTTGAG GACGCACGTC GCCTTAAAGC AATTTATGAA AAAAAGAAAA ATGAACTTGG 2881 CTTATCCCAG GAATCTGTCG CAGACAAGAT GGGGATGGGG CAGTCAGGCG TTGGTGCTTT 2941 ATTTAATGGC ATCAATGCAT TAAATGCTTA TAACGCCGCA TTGCTTACAA AAATTCTCAA 3001 AGTTAGCGTT GAAGAATTTA GCCCTTCAAT CGCCAGAGAA ATCTACGAGA TGTATGAAGC 3061 GGTTAGTATG CAGCCGTCAC TTAGAAGTGA GTATGAGTAC CCTGTTTTTT CTCATGTTCA 3121 GGCAGGGATG TTCTCACCTG AGCTTAGAAC CTTTACCAAA GGTGATGCGG AGAGATGGGT 3181 AAGCACAACC AAAAAAGCCA GTGATTCTGC ATTCTGGCTT GAGGTTGAAG GTAATTCCAT 3241 GACCGCACCA ACAGGCTCCA AGCCAAGCTA GCTTCACGCT GCCGCAAGCA CTCAGGGCGC 3301 AAGGGCTGCT AAAGGAAGCG GAACACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC 3361 CGGATGAATG TCAGCTACTG GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG 3421 CAGGTAGCTT GCAGTGGGCT TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA 3481 AGCGAACCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA 3541 AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGATCAAGA TCTGATCAAG 3601 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 3661 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 3721 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 3781 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 3841 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 3901 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 3961 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 4021 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 4081 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 4141 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 4201 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 4261 GTGTGGCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 4321 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 4381 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 4441 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT 4501 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG 4561 GATCTCATGC TGGAGTTCTT CGCCCACCCC GGGCTCGATC CCCTCGCGAG TTGGTTCAGC 4621 TGCTGCCTGA GGCTGGACGA CCTCGCGGAG TTCTACCGGC AGTGCAAATC CGTCGGCATC 4681 CAGGAAACCA GCAGCGGCTA TCCGCGCATC CATGCCCCCG AACTGCAGGA GTGGGGAGGC 4741 ACGATGGCCG CTTTGGTCGG ATCAATTCGC GCGACCGATC GATCCGGGCA ACGTTGTTGC 4801 CATTGCTGCA GGGGGGGGGG GGGGGGGGGG GGGGGGTTGA CTTCCATTGT TCATTCCACG 4861 GACAAAAACA GAGAAAGGAA ACGACAGAGG CCAAAAAGCC TCGCTTTCAG CACCTGTCGT 4921 TTCCTTTCTT TTCAGAGGGT ATTTTAAATA AAAACATTAA GTTATGACGA AGAAGAACGG 4981 AAACGCCTTA AACCGGAAAA TTTTCATAAA TAGCGAAAAC CCGCGAGGTC GCCGCCCGTA 5041 ACCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT ACATATCACA 5101 ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG TATCGT // 1447 LOCUS ADDGENE_1447 3811 bp DNA circular SYN 01/03/2005 DEFINITION pPD49_78 complete plasmid sequence KEYWORDS . SOURCE pPD49_78 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1447 at www.addgene.org FEATURES Location/Qualifiers source 1..3811 /organism="Addgene plasmid pPD49_78" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 528..545 /label="rf21.28" promoter 1725..1753 /label="Amp_promoter" CDS 1795..2655 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1795..2655 /label="Ampicillin" /gene="Ampicillin" rep_origin 2810..3429 /label="pBR322_origin" misc_feature 3645..3671 /label="lacI.C" promoter 3738..3767 /label="lac_promoter" misc_feature 3802..3820 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGCTAGCGT 541 CGACGGTACC ATGGTATTGA TATCTGAGCT CCGCATCGGC CGCTGTCATC AGATCGCCAT 601 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 661 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 721 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 781 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 841 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 901 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 961 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 1021 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 1081 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 1141 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 1201 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 1261 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 1321 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 1381 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 1441 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 1501 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 1561 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 1621 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 1681 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 1741 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 1801 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 1861 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 1921 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 1981 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 2041 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 2101 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 2161 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 2221 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 2281 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 2341 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 2401 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 2461 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 2521 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 2581 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 2641 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 2701 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 2761 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 2821 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 2881 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 2941 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 3001 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 3061 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 3121 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 3181 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 3241 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 3301 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 3361 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 3421 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 3481 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 3541 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 3601 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 3661 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 3721 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 3781 AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1448 LOCUS ADDGENE_1448 3818 bp DNA circular SYN 01/03/2005 DEFINITION pPD49_83 complete plasmid sequence KEYWORDS . SOURCE pPD49_83 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1448 at www.addgene.org FEATURES Location/Qualifiers source 1..3818 /organism="Addgene plasmid pPD49_83" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 535..552 /label="rf21.28" promoter 1732..1760 /label="Amp_promoter" gene 1802..2662 /label="Ampicillin" /gene="Ampicillin" CDS 1802..2662 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2817..3436 /label="pBR322_origin" misc_feature 3652..3678 /label="lacI.C" promoter 3745..3774 /label="lac_promoter" misc_feature 3809..3827 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGCGTCGA CGGTACCATG GTATTGATAT CTGAGCTCCG CATCGGCCGC TGTCATCAGA 601 TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA 661 TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA 721 TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA 781 AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA 841 TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG 901 ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC 961 GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA 1021 ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT 1081 TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT 1141 TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA 1201 TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA 1261 GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA 1321 ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT 1381 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG 1441 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC 1501 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA 1561 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT 1621 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC 1681 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT 1741 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG 1801 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC 1861 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 1921 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC 1981 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC 2041 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT 2101 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT 2161 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT 2221 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT 2281 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT 2341 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC 2401 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 2461 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 2521 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA 2581 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC 2641 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA 2701 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 2761 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 2821 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 2881 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 2941 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT 3001 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 3061 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 3121 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 3181 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC 3241 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 3301 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 3361 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3421 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3481 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 3541 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA 3601 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 3661 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 3721 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 3781 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1449 LOCUS ADDGENE_1449 7017 bp DNA circular SYN 01/03/2005 DEFINITION pPD50_14 complete plasmid sequence KEYWORDS . SOURCE pPD50_14 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1449 at www.addgene.org FEATURES Location/Qualifiers source 1..7017 /organism="Addgene plasmid pPD50_14" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..3731 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 684..3728 /label="lacZ" /gene="lacZ" promoter 4931..4959 /label="Amp_promoter" gene 5001..5861 /label="Ampicillin" /gene="Ampicillin" CDS 5001..5861 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6016..6635 /label="pBR322_origin" misc_feature 6851..6877 /label="lacI.C" promoter 6944..6973 /label="lac_promoter" misc_feature 7008..7026 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg 601 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg 661 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg 841 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg 901 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg CCCATCTACA 961 CCAACGTGAC CTATCCCATT ACGgtcaatc cgccgtttgt tcccacggag aatccgacgg 1021 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa 1081 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt 1141 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag 1201 aaaaccgcct cgcGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTatctg gaagatcagg 1261 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac 1321 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg 1381 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat 1441 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg 1501 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt 1561 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca 1621 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc 1681 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc 1741 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc 1801 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc 1861 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg 1921 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa 1981 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg 2041 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt 2101 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc 2161 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca 2221 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg 2281 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc 2341 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa 2401 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt 2461 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa 2521 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg 2581 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta 2641 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg 2701 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag 2761 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc 2821 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga 2881 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc 2941 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc 3001 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca 3061 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag 3121 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg 3181 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta 3241 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc 3301 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag 3361 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc 3421 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga 3481 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc 3541 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag 3601 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc 3661 cgtcagtatc ggcggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt 3721 gtcaaaaata ataggcctaC TAGCGTCGAC GGTACCATGG TATTGATATC TGAGCTCCGC 3781 ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 3841 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 3901 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 3961 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 4021 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 4081 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 4141 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 4201 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 4261 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 4321 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 4381 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 4441 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 4501 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 4561 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 4621 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 4681 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 4741 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 4801 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 4861 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 4921 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 4981 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 5041 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 5101 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 5161 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 5221 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 5281 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 5341 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 5401 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 5461 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 5521 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 5581 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 5641 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 5701 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 5761 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 5821 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 5881 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 5941 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6001 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 6061 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 6121 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 6181 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 6241 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 6301 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 6361 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 6421 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 6481 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 6541 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 6601 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 6661 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 6721 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 6781 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 6841 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 6901 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 6961 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1450 LOCUS ADDGENE_1450 7024 bp DNA circular SYN 01/03/2005 DEFINITION pPD50_21 complete plasmid sequence KEYWORDS . SOURCE pPD50_21 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1450 at www.addgene.org FEATURES Location/Qualifiers source 1..7024 /organism="Addgene plasmid pPD50_21" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..3738 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVGEDQKQHLE LSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHP LWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVI IWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPF PAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDW VDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQ FRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELP QPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSE MDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRID PNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDG SGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRW DLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLL HAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 691..3735 /label="lacZ" /gene="lacZ" promoter 4938..4966 /label="Amp_promoter" CDS 5008..5868 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5008..5868 /label="Ampicillin" /gene="Ampicillin" rep_origin 6023..6642 /label="pBR322_origin" misc_feature 6858..6884 /label="lacI.C" promoter 6951..6980 /label="lac_promoter" misc_feature 7015..7033 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt 661 ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac aacgtcgtga ctgggaaaac 721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 841 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt 901 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgCCC 961 ATCTACACCA ACGTGACCTA TCCCATTACG gtcaatccgc cgtttgttcc cacggagaat 1021 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag 1081 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg 1141 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc 1201 gccggagaaa accgcctcgc GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTatctggaa 1261 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg 1321 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct 1381 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt 1441 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt 1501 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg 1561 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc 1621 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa 1681 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag 1741 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg 1801 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg 1861 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac 1921 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa 1981 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg 2041 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc 2101 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt 2161 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg 2221 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac 2281 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag 2341 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat 2401 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat 2461 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg 2521 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg 2581 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg 2641 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag 2701 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta 2761 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag 2821 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg 2881 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt 2941 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg 3001 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg 3061 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc 3121 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc 3181 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt 3241 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat 3301 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc 3361 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg 3421 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc 3481 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac 3541 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg 3601 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc 3661 tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca ttaccaactt 3721 gtctggtgtc aaaaataata ggcctaCTAG CGTCGACGGT ACCATGGTAT TGATATCTGA 3781 GCTCCGCATC GGCCGCTGTC ATCAGATCGC CATCTCGCGC CCGTGCCTCT GACTTCTAAG 3841 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 3901 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 3961 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 4021 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 4081 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 4141 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 4201 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 4261 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 4321 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 4381 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 4441 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 4501 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 4561 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 4621 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 4681 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 4741 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 4801 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 4861 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 4921 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 4981 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 5041 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 5101 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 5161 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 5221 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 5281 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 5341 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 5401 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 5461 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 5521 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 5581 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 5641 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 5701 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 5761 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 5821 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 5881 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 5941 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 6001 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 6061 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 6121 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 6181 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 6241 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 6301 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 6361 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 6421 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 6481 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 6541 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 6601 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 6661 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 6721 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 6781 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 6841 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 6901 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 6961 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 7021 AGCT // 1451 LOCUS ADDGENE_1451 4263 bp DNA circular SYN 01/03/2005 DEFINITION pPD52_102 complete plasmid sequence KEYWORDS . SOURCE pPD52_102 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1451 at www.addgene.org FEATURES Location/Qualifiers source 1..4263 /organism="Addgene plasmid pPD52_102" /mol_type="other DNA" misc_feature 48..70 /label="mec7.-836" misc_feature 860..883 /label="mec7.Tsa" misc_feature 932..973 /label="SynIVS.A" misc_feature 980..997 /label="rf21.28" promoter 2177..2205 /label="Amp_promoter" gene 2247..3107 /label="Ampicillin" /gene="Ampicillin" CDS 2247..3107 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3262..3881 /label="pBR322_origin" misc_feature 4097..4123 /label="lacI.C" promoter 4190..4219 /label="lac_promoter" misc_feature 4254..4272 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTctagAAAT GTAAACCTGT 61 CATTTCTGTG TATTTCAGCA CCGCGAAGAG CACCATAAAC AGCTACAACA AGAGCACGAA 121 CGTCGGCTCC AACAGAAAAA GCTGCAAAAC GCTGGTACTC ACCACATGCT CTGATAATTG 181 CCATTTCCTT CGATTTCTGA CTTTCAATTG TGATATGATA ACCTCAAAAT CTCCATTCAA 241 AATGAAAACT CTTAAATTTA AGAGCTTTCA ACACCCCGCA CTTTCCCACC TCATCGTTTT 301 TCCCTTCTAT CTTATCAATT TTTTGTATTT TGTGTCAGTT TGCTATATCC TCATCACCTT 361 TTTTACCGTT TAATCTCTCT ATAGTATTGT TCGGGTGTTT CAAGATGAAA CGTTTTGTGT 421 GTAGCATTTT TGAAACACAG CGCGAAAAAT GCAGAAACTA TCATCCACAA TGCATTAAGT 481 GCACTCATTG TGATGACTCC TAACCCCCTC GCCCCACCAG TTGTCTCTTC ACAAATTCAT 541 GGCACAATTC AAAAATGGCT TTTTGTGTTG GATCTTGATC TCGTCTCTTT CCTTCTTCTT 601 TCGTTTCTCT CGGACCACTC ATTATTCGAA TGCCATGCAT TTGTTGATGA TGCGCAGCGA 661 TGGTCGCACA CACTACAACA GATATGATGG TGGCTGTGCG TGGTGCAGAG CTCATGAATA 721 AGTGATGGGG CACCGAGATG TGACTCCTCC CATTATTCGT CTTGAGTCGC ATCTTTTGTC 781 TTGGTCCGTT GTCAGTCGAG CCTCAAGGCA GCCGTTCCGC CAGTGATCGC CTGCTAAGAA 841 GTTGTAGGTG TAGAGGAAGA AACGTCGGGT CCAAATTTCA AGcaacatcc gcgagatcgt 901 gaatcccgGG GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT AACATAACAT 961 AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCATGGTATT GATATCTGAG 1021 CTCCGCATCG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT 1081 CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT 1141 GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT 1201 AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA 1261 TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT 1321 ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA 1381 CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC 1441 ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT 1501 TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG 1561 GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT 1621 ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG 1681 GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG 1741 ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC 1801 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 1861 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 1921 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 1981 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 2041 CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 2101 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 2161 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 2221 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 2281 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 2341 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 2401 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 2461 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 2521 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 2581 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 2641 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 2701 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 2761 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 2821 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 2881 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 2941 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 3001 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 3061 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 3121 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 3181 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 3241 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 3301 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 3361 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 3421 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 3481 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 3541 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 3601 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 3661 AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 3721 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 3781 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 3841 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 3901 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 3961 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 4021 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 4081 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 4141 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 4201 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 4261 GCT // 1452 LOCUS ADDGENE_1452 5297 bp DNA circular SYN 01/03/2005 DEFINITION pPD57_56 complete plasmid sequence KEYWORDS . SOURCE pPD57_56 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1452 at www.addgene.org FEATURES Location/Qualifiers source 1..5297 /organism="Addgene plasmid pPD57_56" /mol_type="other DNA" misc_feature 29..52 /label="mec3.-1822" misc_feature 1851..1874 /label="mec3.Tsa" misc_feature 1966..2007 /label="SynIVS.A" misc_feature 2014..2031 /label="rf21.28" promoter 3211..3239 /label="Amp_promoter" gene 3281..4141 /label="Ampicillin" /gene="Ampicillin" CDS 3281..4141 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4296..4915 /label="pBR322_origin" misc_feature 5131..5157 /label="lacI.C" promoter 5224..5253 /label="lac_promoter" misc_feature 5288..5306 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTACC CGGAGTAGTT GGCGAAGATA 61 GAAATGGTGT TATCTTATAT AAATTCAGAC TTTCTCTGTT AAAATTTTAA CCACCAAAAA 121 ATATAGAATT TATAAAAAGT TTCATTTTGA AATTTTATCT TTTGGGGCCA TTTCACTACC 181 TTATCAGGAA GAAATGTGTT TCATCAGAAA TTGAATTTTT CATCTTTTCA GAAAGTGTAC 241 CTAATACCGT ATCATGCTCA TATTCACGTG AATAAGGCTT CTGCCCAGTT CTGTGATTCA 301 AGGAAAACTA CGAAAAATGA TCTCATTTTC TTTGATTTAT CACCGAGCTG GTGTGTCATC 361 ACACACAGTC TGTTTCTTGA TAAAAACGCG CAGAAGTTAG AGCATTCATT GTGATTTTGG 421 GTCTTTAATT TCTGTAAAAC AAATAAAGTG ACAAGTTTAT CATCTGCCTA TTTTATCAGT 481 TATATGCATG GTTAGTTGCA AATAAGGGAG TAGAAATTGA CTAAAACTCA AAATGAGCAC 541 TGAAACGAGG GGTTTTGTTA GTTGCGAAAT AACAATTAAT ACAATCTGGT TGATTACTTT 601 GTAAAAGGAT AATAAAATAT GCAAAACTTG GCGTATTCTG AGATTTCATT CCTAAAGACA 661 TTTATCAAAA CAAATGTTTT CTGCAATTTT CTAAATTCTT GGAATTCTGC CCAGCAGCCT 721 CTAATATCGC GTTTTACTTA GAAAATGACT TTTGGTTTCA TTTTCTCATT TCTGGCTTCT 781 ATTTTTCACA AATGCAAATA TTACATTTAA ATTAGTACTT TCATTTCTAC AAAGATCAAA 841 TTTAACATTA TTGACAGGTT TACTGTTATC TTGTGACAGT TCCGTACTGT CAATCAAAAC 901 GAAAATTAAA ACTCATGAAA TATCAATGAA CATAAATTGA AAAAAAAACC CAGTTGAAAG 961 GAGAAAATAA GCGAAACACA CCAACAACTG AGTAGTTACG CTCACAATTT GGAAATGATT 1021 TTGAAAACTC TCTGGACTGC ATTTCAATTT TTAACAAACA ATGAATACAT ACTGATAGTT 1081 TTTGTACTTC TCGGCATTCA GAGGCAACTT CCTGCCAACA ACCTGGCAAC TTATTTAGTG 1141 CCACTAAAAT AAGTTGCCAA ATTGTTGGCA GGAAGTTGCC TCCAAATGCC GAGTTGGAGC 1201 AAATGATTCG ATATGATTTT AAGGATACCT ACGCATGCCT CCTGCCAAGA CACGCACCCA 1261 CCTACTGAAA TGTCGCGGAA TTTTACCTCC CAAACTATAG ATTGGGTGAA ACTTCACTGT 1321 AAAAGCTTTT TTTAAGTAGT AGGCTGGCAG TAATGAAGAC GTCCATACCA TAATTTTTAA 1381 TGTTGTATAG AAAATCTGAA TTCTCAAGTA TAACATTTTC CAATAATTAG CATAGTCAGA 1441 AACTCTCCAC CGCCGTTCCG TTCCTTTTTT TTGTAACTAT GGAGTAATAG AACAAGATTA 1501 TTCAAATTCA AATTTTTATA AAACTTTACA ACGTCCTTTC CATATATTCT TCACACAACA 1561 TTTGAAAAAA CAACAAATTC ATTCGAAATG CATTGCCCAT AATGAATCGA CCGAAAAACA 1621 CAAGTGACCG TCAGGAGATC GATAGAGAGA GCCCGTTCCA ACTAGACAAC TTTTTAGTGC 1681 TTATCCTTAC ACACACTTTC TAGCTTCATA AGAAATGCAT CTATTATCGT CACCATTTGG 1741 AGACACCAGT TTTAGCGCAC ATTAATAATC GATCAGGGTC TAGAGACTCC TGTTGGATTG 1801 GCATGCCACG CCTACATGAC ATCTGGCTCT TGACGGGTGA TTTGTTACAG TGAGTAGCCA 1861 CAATTTCGCG CATTTTCTTC TAAATTTCAC TGTTATTTGT TCTTctgatt tatttcattt 1921 gaactacgga aatcgGATCC CCGGGATTGG CCAAAGGACC CAAAGGTATG TTTCGAATGA 1981 TACTAACATA ACATAGAACA TTTTCAGGAG GACCCTTGGC TAGCGTCGAC GGTACCATGG 2041 TATTGATATC TGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 2101 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 2161 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 2221 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 2281 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 2341 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 2401 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 2461 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 2521 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 2581 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 2641 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 2701 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 2761 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 2821 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 2881 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 2941 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 3001 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 3061 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 3121 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 3181 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 3241 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 3301 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 3361 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 3421 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 3481 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 3541 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 3601 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 3661 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 3721 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 3781 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 3841 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 3901 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 3961 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 4021 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 4081 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 4141 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 4201 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 4261 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 4321 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 4381 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 4441 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 4501 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 4561 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 4621 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4681 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4741 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4801 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4861 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4921 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 4981 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 5041 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 5101 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 5161 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 5221 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 5281 TCACACAGGA AACAGCT // 1453 LOCUS ADDGENE_1453 11960 bp DNA circular SYN 01/03/2005 DEFINITION pPD5_41 complete plasmid sequence KEYWORDS . SOURCE pPD5_41 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1453 at www.addgene.org FEATURES Location/Qualifiers source 1..11960 /organism="Addgene plasmid pPD5_41" /mol_type="other DNA" gene 612..7881 /label="unc54(w/ gaps)" /gene="unc54(w/ gaps)" misc_feature 1696..1726 /label="unc54.enh" CDS complement(2515..3249) /label="Orf0" /translation="MVEELLELLVYEVNPQLLEGVEVEDFETGDIEHTDEIITINTLL IQSEVTLLDKPVEHTGVETLGHGSDSPIDLFAVLSLGYPFGSDLDTWLGQSLEELLAS DSVHVGCLLGLYGTIWLSLLFTWALLELHVTHVHVSGHEAVAILLLDGREVEDVESFI SKLELFDVIDSVNDQLGLGDEPVILDWQVKKKFLLELWTEVRVDLVEDVVATLSWSLT DDTRFLEQVCKIYSLNNSCIIFIELC" CDS 3426..7091 /label="Orf2" /translation="MLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEA HFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAA KAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMI DAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAKSDDDKKKCAEA IMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGL KDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKV KALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKD ASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKD HQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQT LDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELH SVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEEL GEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELT DQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSK ADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTAD EEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGE GLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERAN GVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEV VEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALE AEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAE LLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTR EQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSA KRKLEGEIQAIHVSFRY" CDS 3907..4710 /label="Orf0" /translation="MPLWFSTSLPATECWKESEFAERDSPTEPFIQTSSNVTPSLPPR RPSPMTTRRSAPRLSCPSSSTTDPSARRCSVSVSPRSSSRLEFLLILKTSVTRSSPPS SPDSNPKSDGIWVSRTASAVWNNVPDFSLFSATSVPGALSVPGNGSSFTERSSQCSRP ERKPRSSRRSTTRLRPSKTVSPRRRSFARSSRSPLPSSSKRKLLSSPTWNPPRLNSPM LRKDLPSLRLNKRMPPSNSPSSTINSLTTKTVPLMFSALRRRSRPRSRL" CDS complement(3390..4916) /label="Orf2" /translation="MVTLILFGLEVLHQLAVDFFLMLLLLVELGNSFILLLHLILKGS DLVILGLLLRLSLAERHFQVLDLLLQSLDLGLDLLLSALNISGTVFVVSELIVELGEL LGGILLLSLKLGKSFLSIGELSLGGFQVGEERSFLFDELGRGLLELLAKLLLLGETVF EGLNLVVDLLELLGFLSGLEHWLDLSVKLEPFPGTESAPGTDVALNNEKSGTLFHTAL AVLETQMPSDLGLESGEDGGELLVTDVFKMSKNSSLEEDLGETDTEHLLAEGSVVDEL GHDSLGALLLVVIGLGLLGGKDGVTLDEVWMKGSVGESLSANSDSFQHSVAGKLVENQ SGIDHTRLLLLVGDDTTDEMWVSLVEHSHQVVERLSVEHRDGHERSGLSLLLSTATSL LGLSGGLFLGGVVLPDLDEKIVSLGLFHCRDNGVVEGVLVLLEPVQAVVTHSSGVVPH GEVSFSLLSLWWLWLLEVWMLAKMLIDKLGSEGQISSLWYDTLFIKHGDNTEWLKNKN" promoter complement(9112..9130) /label="T7_promoter" misc_feature 9135..9290 /label="lacZ_a" misc_feature complement(9138..9154) /label="M13f20_primer" rep_origin 9316..9622 /label="f1_origin" promoter 9818..9846 /label="Amp_promoter" gene 9888..10748 /label="Ampicillin" /gene="Ampicillin" CDS 9888..10748 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 10903..11522 /label="pBR322_origin" misc_feature 11738..11764 /label="lacI.C" promoter 11831..11860 /label="lac_promoter" misc_feature 11895..11913 /label="M13r_primer" promoter 11930..11949 /label="T3_promoter" ORIGIN 1 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTGGAT CCCCCAACTA GGTCGTAAAT 61 GTATTGATTT GCTTAAAATC TGAAGATCTA GTGGTGAACC GTGGAAGATT ATCAAGAGGA 121 GGCTGAAGAT CTGTTTAAGA ACCATTAATC AAACTGGTAT TCTATTTTCA CTGGTTGTAT 181 GTAAACATTC TATCTTATTC CTTTTATCAC TGTTCTGCAC TTTCCTATAA AAAAAGTTGA 241 CCGACCGTAC TCTCTGAATT CATTTTTCCC GATCTTACCA ACTCCCGATC TATCTCTATC 301 CCTGGTTTTT TCTTCGTGCT CCAATGGAAT TCTTGAGACT TCCACTATCT TCTCTGGCAC 361 CCTCCACTAC GCGTAGGCGT CTCTCGCTTC GTGTATTCCC GGGAAGCCGG GTTCCCGTCT 421 CTCCCGCCGC TGCCGCTGCC GCACACAGCT TTACACCTCG TAGAATCCCC AAAGAGGGGC 481 GTGGCTTGCG GGTGCCAACA TCCTCCTGCC GAGGAAGAAG CAGGCACTCA TCACTCGCAT 541 CATCAACCTC GACTAAGGGA GCACCACATT AGTTTTGAGG TAGAGAAACC ATTTGAAAGA 601 AGCGAGAAAT CATGGAGCAC GAGAAGGACC CAGGATGGCA ATATCTCCGC CGTACCAGAG 661 AGCAGGTTTT GGAGGTGAGT ATTTATTTTT TATTTATTTA TTTTTCTTTC ATTTGTCTTT 721 ATTGTTATGC TCATTTCTAT AGAGTAAATC GTATAGTACT CCATAAGTAT CTAATTTTAA 781 CAAAACTGAG CATTTATTTT TGAAATTCTA GATTTTTAAC ATACAACTGA GTTAAAGTCT 841 GCCATTTTAC ACTTATAGAT TTTTTAAATT CAGGTAGAAG ATTTGAGCTT GCCTTGTAAT 901 CCTTGTAATC AATGTTTATT TCTGTTTTCT AATTTTTCAT AGATTTAGCT TCAAGATCAT 961 CTTTCCCTTG AAATCATTGA AATCTTCCTT TCTTAAATTT TTGCATTGCA CTTGGAATTT 1021 TGTGACACAT TGTGCTGCAG TAGTACTGTA TTTTGAGACG GCACATTTAT TTTGACTACA 1081 CCACAAGCTT AACCTTGATT TTACTTTTTG ATTCCATTCC ATTCATGGGT CTAAAGTGTG 1141 AAGCGGTTTA GACCATTTTT CAACACAAGT TGGTATTCTT TAGAAGTGAT ATATTTCTGA 1201 ATTCTGATCT CTTTTGTACC ATTATTGTAA TTTCAGGATC AATCTAAGCC TTATGACTCC 1261 AAGAAGAACG TCTGGATCCC AGATCCAGAA GAGGGATACC TTGCCGGAGA AATCACCGCC 1321 ACCAAGGGAG ACCAGGTCAC CATCGTCACC GCTCGCGAAA TGAGGTTCGT TTTTAAGCTA 1381 ATGATTACGG TTTTCAATGT AGTGTGATTC AGGTCACCCT CAAGAAGGAG TTGGTTCAAG 1441 AAATGAATCC ACCAAAGTTC GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG 1501 ACGCCTCTGT CCTCCATAAC TTGCGTTCCC GTTACGCTGC TATGCTCATC TACGTAAGTT 1561 TCATTGAAAC TTAAAGTTAA GCATTGAGAA TCATTCATAT TCTTCTTCTA AATTCCCATA 1621 AAATCCCGAA ACTCCTTCCC TCTATCTTCT TTTTCTTCTC GTTTTCAAAT GTTTCTCTCT 1681 ATCCCATTCT CTCATCAATT GAGTGGGATG AGGCTATCTC TGCCTCTCTT CTGAATCTCT 1741 GAACCATCTT ACATTACACT GTGGATGACG AGCCCCACAG GCTCCCTTGC ATCAGATACT 1801 GCCATTGGGG ATGGCAAAGA AGAGAGAAGG TATTGTGAGG ATATATTTTT CTAAGAAAAA 1861 ACGTTTGAAG AAAAGAAGAT GAAGAAGATC TGCTTGATTC ATTGCACAAG TTAGAAGTAA 1921 CAGGGGTCTA TATTTCGAAG AACTTAAAGG GAATGCAACT GAACATAAAA TTAAACAAAG 1981 GGATTGAATC CTGCAGTATT TAAAAATCGA AACTAGAATG TTTCCTATTT CAGACCTACT 2041 CCGGACTTTT CTGCGTTGTC ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT 2101 GCGCCCGTAT GTTCATGGGA AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT 2161 CTGACGAAGC CTACCGTAAC ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG 2221 GAGAATCTGG AGCCGGAAAG ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG 2281 TCGGTGCCTC CCAACAGGAA GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC 2341 TTGAGGACCA AATCGTCCAG ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG 2401 TCCGTAACAA CAACTCATCC CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG 2461 GACGCCTCGC ATCTTGCGAT ATCGAGCATT GTGAGTTACT TTTTGATTTA TATTCTAGCA 2521 TAGCTCAATA AAAATTATGC AACTATTGTT TAAACTATAA ATTTTGCAGA CTTGCTCGAG 2581 AAATCTCGTG TCATCCGTCA AGCTCCAGGA GAGCGTTGCT ACCACATCTT CTACCAAATC 2641 TACTCTGACT TCCGTCCAGA GCTCAAGAAG GAACTTCTTC TTGACTTGCC AATCAAGGAT 2701 TACTGGTTCG TCGCCCAGGC CGAGTTGATC ATTGACGGAA TCGATGACGT CGAAGAGTTC 2761 CAACTTACTG ATGAAGCTTT CGACATCCTC AACTTCTCTG CCGTCGAGAA GCAGGATTGC 2821 TACCGCCTCA TGTCCGCTCA CATGCACATG GGTAACATGA AGTTCAAGCA ACGCCCACGT 2881 GAAGAGCAAG CTGAGCCAGA TGGTACCGTA GAGGCCGAGA AGGCATCCAA CATGTACGGA 2941 ATCGGATGCG AGGAGTTCCT CAAGGCTTTG ACCAAGCCAC GTGTCAAGGT CGGAACCGAA 3001 TGGGTATCCA AGGGACAGAA CTGCGAACAA GTCAATTGGG CTGTCGGAGC CATGGCCAAG 3061 GGTCTCTACT CCCGTGTGTT CAACTGGCTT GTCAAGAAGT GTAACCTCAC TCTGGATCAA 3121 AAGGGTATTG ATCGTGATTA TTTCATCGGT GTGCTCGATA TCGCCGGTTT CGAAATCTTC 3181 GACTTCAACT CCTTCGAGCA GTTGTGGATT AACTTCGTAA ACGAGAAGCT CCAACAATTC 3241 TTCAACCATC ACATGTTCGT CCTTGAACAG GAAGAATACG CCCGTGAGGG TATTCAATGG 3301 GTCTTCATCG ATTTCGGACT TGATTTGCAA GCGTGTATCG AACTTATTGA GAAGGTAAGA 3361 TTGAATTGAA ATTTTAAATG TTATTTTGAT CAATTCTTAT TTTTCAGCCA CTCGGTATTA 3421 TCTCCATGCT TGATGAAGAG TGTATCGTAC CAAAGGCTAC TGATTTGACC CTCGCTTCCA 3481 AGCTTGTCGA TCAACATCTT GGCAAGCATC CAAACTTCGA GAAGCCAAAG CCACCAAAGG 3541 GAAAGCAAGG AGAAGCTCAC TTCGCCATGC GGCACTACGC CGGAACTGTG CGTTACAACT 3601 GCTTGAACTG GCTCGAGAAG AACAAGGACC CCCTCAACGA CACCGTTGTC TCGGCAATGA 3661 AACAATCCAA GGGAAACGAT CTTCTCGTCG AGATCTGGCA AGACTACACC ACCCAGGAAG 3721 AGGCCGCCGC TAAGGCCAAG GAGGGAGGTG GCGGTGGAAA GAAGAAGGGA AAGTCCGGAT 3781 CTTTCATGAC CGTCTCTATG CTCTACAGAG AGTCTCTCAA CAACTTGATG ACTATGCTCA 3841 ACAAGACTCA CCCACATTTC ATCCGTTGTA TCATCCCCAA CGAGAAGAAG CAATCTGGTA 3901 TGATCGATGC CGCTTTGGTT CTCAACCAGC TTACCTGCAA CGGAGTGTTG GAAGGAATCA 3961 GAATTTGCAG AAAGGGATTC CCCAACAGAA CCCTTCATCC AGACTTCGTC CAACGTTACG 4021 CCATCCTTGC CGCCAAGGAG GCCAAGTCCG ATGACGACAA GAAGAAGTGC GCCGAGGCTA 4081 TCATGTCCAA GCTCGTCAAC GACGGATCCC TCAGCGAGGA GATGTTCCGT ATCGGTCTCA 4141 CCAAGGTCTT CTTCAAGGCT GGAGTTCTTG CTCATCTTGA AGACATCCGT GACGAGAAGC 4201 TCGCCACCAT CCTCACCGGA TTCCAATCCC AAATCAGATG GCATTTGGGT CTCAAGGACC 4261 GCAAGCGCCG TATGGAACAA CGTGCCGGAC TTCTCATTGT TCAGCGCAAC GTCCGTTCCT 4321 GGTGCACTCT CCGTACCTGG GAATGGTTCA AGCTTTACGG AAAGGTCAAG CCAATGCTCA 4381 AGGCCGGAAA GGAAGCCGAG GAGCTCGAGA AGATCAACGA CAAGGTTAAG GCCCTCGAAG 4441 ACAGTCTCGC CAAGGAGGAG AAGCTTCGCA AGGAGCTCGA GGAGTCCTCT GCCAAGCTCG 4501 TCGAAGAGAA AACTTCTCTC TTCACCAACT TGGAATCCAC CAAGACTCAA CTCTCCGATG 4561 CTGAGGAAAG ACTTGCCAAG CTTGAGGCTC AACAAAAGGA TGCCTCCAAG CAACTCTCCG 4621 AGCTCAACGA TCAACTCGCT GACAACGAAG ACCGTACCGC TGATGTTCAG CGCGCTAAGA 4681 AGAAGATCGA GGCCGAGGTC GAGGCTTTGA AGAAGCAGAT CCAAGACTTG GAAATGTCTC 4741 TCCGCAAGGC TGAGTCTGAG AAGCAATCCA AGGATCACCA GATCAGATCC CTTCAAGATG 4801 AGATGCAACA GCAAGATGAA GCTATTGCCA AGCTCAACAA GGAGAAGAAG CATCAAGAAG 4861 AAATCAACCG CAAGCTGATG GAGGACCTCC AATCCGAAGA GGATAAGGGT AACCATCAGA 4921 ACAAGGTCAA GGCCAAGCTT GAGCAGACTC TTGATGACCT CGAGGATTCC CTTGAGCGCG 4981 AGAAGAGAGC CCGCGCCGAT CTTGACAAGC AGAAGAGAAA GGTCGAGGGA GAGCTCAAGA 5041 TTGCTCAAGA GAACATCGAT GAGAGCGGAC GCCAACGCCA CGATCTTGAG AACAACTTGA 5101 AGAAGAAGGA ATCTGAGTTG CACTCAGTTT CTTCCCGACT TGAGGACGAA CAAGCTCTTG 5161 TCTCCAAGCT CCAACGCCAG ATCAAGGACG GACAAAGCCG TATCTCCGAG CTCGAAGAGG 5221 AACTCGAGAA TGAACGTCAA TCCCGTTCCA AGGCTGACCG TGCCAAGAGC GACCTCCAAC 5281 GCGAGTTGGA AGAGCTCGGT GAGAAGCTTG ACGAGCAAGG TGGAGCTACT GCCGCCCAGG 5341 TTGAGGTCAA CAAGAAGCGT GAGGCTGAAC TTGCCAAGCT CCGCAGAGAC TTGGAGGAGG 5401 CCAACATGAA CCACGAGAAC CAACTCGGTG GACTTCGCAA GAAGCACACC GACGCTGTCG 5461 CTGAGCTCAC CGACCAACTC GATCAACTCA ACAAGGCCAA GGCTAAGGTC GAGAAGGACA 5521 AGGCTCAAGC TGTTCGTGAC GCTGAGGACC TTGCTGCTCA ACTTGACCAA GAAACCTCTG 5581 GAAAGCTCAA CAACGAAAAG CTTGCCAAGC AGTTCGAACT TCAACTCACC GAGCTTCAAT 5641 CTAAGGCTGA TGAGCAATCT CGCCAACTCC AAGACTTCAC TTCCCTTAAG GGACGCCTTC 5701 ACTCTGAGAA TGGAGACCTT GTCCGTCAAC TCGAAGATGC CGAATCCCAA GTCAACCAAC 5761 TCACCAGACT GAAGTCCCAG CTTACTTCTC AACTCGAGGA GGCTCGCCGC ACCGCTGACG 5821 AGGAAGCACG CGAACGTCAA ACCGTCGCCG CCCAGGCTAA GAACTACCAA CACGAAGCCG 5881 AGCAACTCCA AGAGTCCCTT GAAGAGGAGA TCGAAGGAAA GAACGAGATC CTCAGACAAC 5941 TCTCCAAGGC TAACGCCGAC ATCCAACAAT GGAAGGCTCG CTTCGAGGGA GAAGGACTCC 6001 TCAAGGCCGA CGAGCTCGAG GATGCCAAGA GACGCCAAGC CCAAAAGATC AACGAGCTCC 6061 AAGAGGCTCT TGATGCCGCC AACTCTAAGA ACGCTTCTCT TGAGAAGACC AAGTCCAGAC 6121 TTGTCGGAGA CTTGGACGAC GCTCAAGTTG ATGTCGAGAG AGCCAACGGT GTCGCCAGTG 6181 CTCTTGAGAA GAAGCAAAAG GGCTTCGACA AGATCATCGA CGAATGGAGA AAGAAGACCG 6241 ACGACTTGGC CGCTGAGCTT GATGGAGCTC AACGCGACCT TCGCAACACC TCCACTGATC 6301 TCTTCAAGGC CAAGAACGCC CAAGAGGAGC TCGCCGAGGT TGTTGAGGGA CTCCGCCGTG 6361 AGAACAAGAG CTTGAGCCAA GAGATCAAGG ATCTTACCGA TCAACTCGGA GAGGGAGGAC 6421 GCTCTGTCCA CGAAATGCAA AAGATCATCC GCCGTCTTGA GATTGAGAAG GAAGAACTCC 6481 AACACGCTTT GGACGAGGCT GAGGCTGCCC TTGAAGCTGA AGAGAGCAAG GTTCTCCGCG 6541 CCCAGGTTGA AGTTTCCCAG ATCCGTTCCG AAATCGAGAA ACGCATCCAG GAGAAGGAGG 6601 AAGAGTTCGA GAACACGAGA AAGAACCACG CCCGCGCTCT TGAATCAATG CAAGCTTCCC 6661 TCGAGACCGA AGCTAAAGGA AAGGCCGAAC TTCTCCGCAT CAAGAAGAAG CTCGAGGGAG 6721 ATATCAACGA GCTCGAGATC GCTTTGGACC ACGCCAACAA GGCTAACGCC GATGCCCAGA 6781 AGAACTTGAA GAGATACCAA GAGCAAGTCC GCGAGTTGCA ATTGCAAGTC GAGGAGGAGC 6841 AACGCAATGG AGCCGACACC CGTGAACAAT TCTTCAACGC CGAGAAGCGC GCCACTCTTC 6901 TTCAATCCGA GAAGGAAGAA CTTTTGGTTG CCAACGAGGC CGCCGAGAGA GCCCGCAAGC 6961 AAGCCGAGTA TGAAGCCGCC GATGCTCGTG ATCAAGCCAA CGAAGCCAAC GCTCAAGTCA 7021 GCAGCTTGAC TTCTGCCAAG AGAAAGCTCG AGGGAGAAAT CCAAGCCATT CATGTAAGTT 7081 TTAGATATTG ACAATCTAGT TTTTCAGTCT ATTCAATGTG AATCTTTCAG GCCGACCTCG 7141 ATGAGACCCT CAACGAGTAC AAGGCCGCTG AGGAGCGCTC CAAGAAGGCT ATTGCTGATG 7201 CCACCAGACT CGCAGAGGAG CTCCGTCAAG AACAAGAACA CTCTCAGCAC GTTGACCGTC 7261 TTCGCAAGGG ACTTGAGCAA CAGCTCAAGG AGATCCAAGT CCGTCTTGAT GAGGCCGAGG 7321 CTGCTGCTCT TAAGGGAGGA AAGAAGGTAA TACATCATAT CGTTCAAAAT ATATACAAAT 7381 TTCATGGTTT TCAGGTTATC GCCAAGCTCG AACAACGCGT GCGTGAGCTC GAATCTGAAT 7441 TGGACGGAGA ACAACGCCGC TTCCAGGATG CCAACAAGAA CCTCGGACGC GCCGATAGAC 7501 GTGTTCGTGA GCTTCAATTC CAGGTTGATG AGGACAAGAA GAACTTCGAA CGTCTCCAAG 7561 ATCTCATTGA CAAGCTCCAA CAGAAGTTGA AGACCCAGAA GAAGCAGGTT GAGGAAGCTG 7621 TAAGTTTATT ATATCTTGTA ACCTGACATT CCATCATCTT ATTAATTTCA GGAGGAACTC 7681 GCCAACTTGA ACCTCCAGAA GTACAAGCAA CTCACCCACC AACTTGAAGA TGCTGAGGAG 7741 CGCGCCGATC AAGCCGAAAA CTCCCTCTCA AAGATGCGAT CAAAATCCCG TGCATCCGCC 7801 TCTGTTGCCC CAGGACTCCA GAGCTCCGCA TCGGCCGCTG TCATCAGATC GCCATCTCGC 7861 GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC 7921 CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT 7981 TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT 8041 TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC 8101 AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT 8161 GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT 8221 GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC 8281 GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA 8341 TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG 8401 TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT 8461 TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT 8521 CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT 8581 AAAATTAAAC ATTTTTCTAA ATTTTATATG ATTTCTTTTA AATTTGCAAA AATTACTTAA 8641 ATTTGAATTC CCGCGCAAAT GAGTGACTTC ATTTTCTGCA TTATTGTGTT TTCCGGCTAT 8701 ATTAATAGGT ATTTGTTTGT GTTTTTCTTT ATTTTATGAT TCGAACTCCA ATTTGTAAAT 8761 TTTCGAACAT ATTTCCCTAA AGAAAAAATA TGATTAATCT GGAAAAATTG GAAAATTATT 8821 TTTCAAATAA AAAACAAAGA AAAAAATGAA GAAAAACCTA TTAGTTTGGC CATAAAACGC 8881 AAAAATGTCG AAAATGACGT CACTCATCTG CGCGGGAAAT CAAGAATAAT TCGGCCTTTT 8941 TTATTTTTTT GGAAAATCGT AAAACATTTA GAAAAATTTT TTAATAGTTA TATGGGACTT 9001 ATTCTGCATT TAGGGCAAAA GCCAGAGACT ACTCCACCGT TGGGCTGCAG GAATTCGATA 9061 TCAAGCTTAT CGATACCGTC GACCTCGAGG GGGGGCCCGG TACCCAATTC GCCCTATAGT 9121 GAGTCGTATT ACAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 9181 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 9241 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCGAC 9301 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 9361 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 9421 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 9481 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 9541 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 9601 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 9661 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 9721 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCCCAGG TGGCACTTTT CGGGGAAATG 9781 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 9841 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 9901 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 9961 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 10021 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 10081 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 10141 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 10201 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 10261 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 10321 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 10381 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 10441 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 10501 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 10561 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 10621 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 10681 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 10741 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 10801 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 10861 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 10921 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 10981 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 11041 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 11101 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 11161 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 11221 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 11281 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 11341 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 11401 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 11461 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 11521 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 11581 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 11641 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 11701 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 11761 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 11821 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 11881 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTCGGA ATTAACCCTC 11941 ACTAAAGGGA ACAAAAGCTG // 1454 LOCUS ADDGENE_1454 8217 bp DNA circular SYN 01/03/2005 DEFINITION pPD69_39 complete plasmid sequence KEYWORDS . SOURCE pPD69_39 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1454 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid pPD69_39" /mol_type="other DNA" misc_feature 42..66 /label="glp1.delta-1635" misc_feature 1651..1678 /label="glp1.Tsa" CDS 1763..4927 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 1763..1789 /label="SV40.NLS" gene 1880..4924 /label="lacZ" /gene="lacZ" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGCCTT GGTCGAGTAT GAGAAACAAC 61 ATTAACAATG TTTTGGGAGA AACATGTAGT TTTGTACGTC CGGTCCAGTG AGAGTTTTCA 121 ACTTATTTTG GAAGAAATTT TTTTTTGACA AAATTTTGCA ATTTCCCACG CAGAAATACG 181 GTATGCCCCG TATCTCGCCA CGAACATAAT ATTTTAAATT GACGGAAAGG TGTGCGCCTT 241 TAAGAAGCAC AGTAACCATT TTCCGGTTTT TTTTTTGAAA TTGCCTTTGA TTTTTACTTT 301 TTTCTCCCAA AAACGAGAAA TTTAATATAA TCCATCAAAA TCTTTGTGAA CGAGAGAAAT 361 ATAGTGAAAA ACTCAAAGAA AATTGCGAAA TGGATTACTG TACTCCATAA AGGCGCACAT 421 CTTTTCGAAA AATCGTGAAA AATTGCATCT GACCAACAGT TTTGCGTGGA ATCTTCCTAG 481 ATTCAAATTT CCTATGAGTC ATAAAACTAT CATTTTATCG GACAATAACT ACACGTGTAC 541 CTTGTTTCCA TATGTTCTAC CAATATAATT TGTGTTCATA TGTGGCCAGG TACGGGAAAT 601 TAGAACACGG ACTGATTTAG GAAGGTTCAA ATTCGAGAAC AATGGACGAC TTGCTGCACG 661 AAAACGTGGA TTATTCTGAA ACCGCCTATG TGATCACTAT CGATTTAGCA CTTCGGTTTT 721 CTTTTTCCGT TTTCCTTTAT ATTCCGTATT GTATGCCGAC AATAACGCTT TTTCTTTGTA 781 TAAACATTTC CAGAAGCAGA GCAAATTTGT TTATTCCAGA ATTGACTGAA TATTGAAATA 841 ATGTTTACAG ACATACTGTA GATTTTTTTC TATTCAAACA TGAATACAGT TTTTCGTATT 901 TTTAATCTTC AGATAAGTTT TGTGTGTGTT TTTGAGAATA ATATTTAAAA AAGATAAGCA 961 AATATCAGAA CTACGGTAAG CGAAGGGGTA CGGTCGTCAA TTGCGGACTT CCGAGCTGTG 1021 CACGGCGCCA AAATTTATTT TGAGTTTTAA AATGATTTTC TTTGTTTTTT TTTCTTTTTT 1081 TGACACGATA AGAACATTTT TCTGTATTTC TAATATTTTA AATGAGATCG TAATAAGTTT 1141 ATTTAGCTCC ATCACATTAT TTTCGGAATA TTCATCGCTT GAAAACGGCG TAAAATAGGT 1201 TCCAAGTAAA TCATTGCAAA CAAATCTCCG TTTATCGTTC TAACAAAGTT TTTCGTTCAC 1261 TTTCTTATGT AAATCGTTTT CCCCACTTCT TCTTCACGGG AATGATCAAT TGAAAGGCCA 1321 TAAATTGGTT GTTTTCTCCA CATTTCGCAA CCAACATGCA GCTGTCGGAT AGGCAATAAA 1381 ATAATGTAAT CAATGAGTTT TCCGATTTAT CCTAACAGTT CTTTTTCTAG TGTTCCTATT 1441 CGAAAAAAGT ACTGTTATCC TTCTTTTTGG ACCGCCCATC TCAGGTGTCA AGACAATCGC 1501 AACACACTCA GAAAATGGGC GGAGTATGCC ACCCGGCCTT CATTCGTCGC TCCTTGTCTT 1561 CTCCATTTTC GTTTTTTTCC TGTTCTCCGT TCTTTTTTTG TACTTTTGTG ATAATGCTGA 1621 AGTGTTCTTT TTTTTCGCTT AACCGCATCT CTTGATTTAA TTCATATTTT CTTTCAGATT 1681 CATaTACAAT AACACCAAGA GCTGCTCTAA CAACTAATAA TAACAGAATC TAGAGGATCC 1741 CCGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGTGGG 1801 TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT 1861 GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 1921 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 1981 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCTTTGCCTG 2041 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 2101 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 2161 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 2221 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 2281 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 2341 CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA 2401 CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT 2461 GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT 2521 CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG TACTGGAGGC 2581 TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT CTTTATGGCA 2641 GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA TCGATGAGCG 2701 TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA AACTGTGGAG 2761 CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG ACGGCACGCT 2821 GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA ATGGTCTGCT 2881 GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC ATCATCCTCT 2941 GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA 3001 CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG 3061 CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC 3121 AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG 3181 AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGAATC 3241 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3301 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3361 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3421 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG CCCACGCGAT 3481 GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT 3541 ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG 3601 CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG 3661 TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG AAGCAAAACA 3721 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 3781 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 3841 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 3901 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 3961 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC AGTGGCGTCT 4021 GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC 4081 CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC 4141 AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA 4201 TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA 4261 CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG AAGCAGCGTT 4321 GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG CTCACGCGTG 4381 GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG ATGGTAGTGG 4441 TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC CGGCGCGGAT 4501 TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG GATTAGGGCC 4561 GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG ATCTGCCATT 4621 GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG 4681 CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA 4741 CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC 4801 ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC 4861 AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA 4921 AAAATAATAG GCCTActagc gtcgacggta cCATGCATGg tattgatatc tgagctccgc 4981 atcggccgct gtcatcagat cgcCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT Tcctactagt cggccgtacg GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1455 LOCUS ADDGENE_1455 3633 bp DNA circular SYN 01/03/2005 DEFINITION pPD79_44 complete plasmid sequence KEYWORDS . SOURCE pPD79_44 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1455 at www.addgene.org FEATURES Location/Qualifiers source 1..3633 /organism="Addgene plasmid pPD79_44" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1278 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHM KRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVL LPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPGDPLVLERPPPR WSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ QLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQA LNRGLPLGFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 103..810 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 286..309 /label="gfp.S65S" promoter complement(874..892) /label="T7_promoter" misc_feature 912..1060 /label="lacZ_a" misc_feature complement(903..919) /label="M13f20_primer" rep_origin 1078..1384 /label="f1_origin" promoter 1577..1605 /label="Amp_promoter" CDS 1647..2507 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1647..2507 /label="Ampicillin" /gene="Ampicillin" rep_origin 2662..3281 /label="pBR322_origin" misc_feature 3497..3523 /label="lacI.C" promoter 3590..3619 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 121 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 181 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 241 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGCCAACAC TTGTCACTAC TTTCTCTTAT 301 GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT 361 GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC 421 AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT CGAGTTAAAA 481 GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA CAACTATAAC 541 TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT TAACTTCAAA 601 ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA ACAAAATACT 661 CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC ACAATCTGCC 721 CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT TGTAACAGCT 781 GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATGCC CGGGGGATCC ACTAGTTCTA 841 GAGCGGCCGC CACCGCGGTG GAGCTCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC 901 TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 961 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 1021 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA 1081 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 1141 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 1201 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 1261 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 1321 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 1381 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 1441 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA 1501 TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 1561 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 1621 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 1681 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 1741 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 1801 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 1861 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 1921 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 1981 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 2041 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 2101 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 2161 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 2221 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 2281 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 2341 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 2401 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 2461 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 2521 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 2581 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 2641 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 2701 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 2761 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 2821 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 2881 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 2941 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 3001 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 3061 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 3121 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 3181 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 3241 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 3301 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 3361 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 3421 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 3481 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 3541 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 3601 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGA // 1456 LOCUS ADDGENE_1456 8984 bp DNA circular SYN 01/03/2005 DEFINITION pPD80_08 complete plasmid sequence KEYWORDS . SOURCE pPD80_08 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1456 at www.addgene.org FEATURES Location/Qualifiers source 1..8984 /organism="Addgene plasmid pPD80_08" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" CDS 1049..5227 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG KLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTR AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQ LNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEA DTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQT RIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYL EDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLR VTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVV ELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQT MVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDD PRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYE GGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNS LGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCM NGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGK PLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQ WRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLL TPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVL ITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAE RVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQW RGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQL SAGRYHYQLVWCQK" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1334..1360 /label="SV40.NLS" gene 1376..2083 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 1559..1582 /label="gfp.S65S" gene 2180..5224 /label="lacZ" /gene="lacZ" promoter 6898..6926 /label="Amp_promoter" CDS 6968..7828 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6968..7828 /label="Ampicillin" /gene="Ampicillin" rep_origin 7983..8602 /label="pBR322_origin" misc_feature 8818..8844 /label="lacI.C" promoter 8911..8940 /label="lac_promoter" misc_feature 8975..8993 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGgt 1261 atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT GGAGGGTACC 1321 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTaCCGGTAG AAAAAATGAG 1381 TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT 1441 TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT 1501 TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGCCAA CACTTGTCAC 1561 TACTTTCTCT TATGGTGTTC AATGCTTTTC AAGATACCCA GATCATATGA AACgGCATGA 1621 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 1681 TGACGGGAAC TACAAGACAC GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG 1741 AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA 1801 ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA 1861 AGTTAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA 1921 TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC 1981 CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA 2041 GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAT gcccggtGGG 2101 TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT 2161 GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 2221 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 2281 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCTTTGCCTG 2341 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 2401 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 2461 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 2521 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 2581 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 2641 CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA 2701 CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT 2761 GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT 2821 CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG TACTGGAGGC 2881 TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT CTTTATGGCA 2941 GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA TCGATGAGCG 3001 TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA AACTGTGGAG 3061 CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG ACGGCACGCT 3121 GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA ATGGTCTGCT 3181 GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC ATCATCCTCT 3241 GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA 3301 CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG 3361 CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC 3421 AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG 3481 AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGAATC 3541 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3601 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3661 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3721 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG CCCACGCGAT 3781 GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT 3841 ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG 3901 CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG 3961 TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG AAGCAAAACA 4021 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 4081 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 4141 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 4201 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 4261 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC AGTGGCGTCT 4321 GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC 4381 CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC 4441 AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA 4501 TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA 4561 CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG AAGCAGCGTT 4621 GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG CTCACGCGTG 4681 GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG ATGGTAGTGG 4741 TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC CGGCGCGGAT 4801 TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG GATTAGGGCC 4861 GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG ATCTGCCATT 4921 GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG 4981 CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA 5041 CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC 5101 ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC 5161 AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA 5221 AAAATAATAG GGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC TGACTTCTAA 5281 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 5341 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 5401 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 5461 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 5521 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 5581 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 5641 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 5701 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 5761 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 5821 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 5881 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 5941 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTA AAATTAAACA TTTTTCTAAA 6001 TTTTATATGA TTTCTTTTAA ATTTGCAAAA ATTACTTAAA TTTGAATTCC CGCGCAAATG 6061 AGTGACTTCA TTTTCTGCAT TATTGTGTTT TCCGGCTATA TTAATAGGTA TTTGTTTGTG 6121 TTTTTCTTTA TTTTATGATT CGAACTCCAA TTTGTAAATT TTCGAACATA TTTCCCTAAA 6181 GAAAAAATAT GATTAATCTG GAAAAATTGG AAAATTATTT TTCAAATAAA AAACAAAGAA 6241 AAAAATGAAG AAAAACCTAT TAGTTTGGCC ATAAAACGCA AAAATGTCGA AAATGACGTC 6301 ACTCATCTGC GCGGGAAATC AAGAATAATT CGGCCTTTTT TATTTTTTTG GAAAATCGTA 6361 AAACATTTAG AAAAATTTTT TAATAGTTAT ATGGGACTTA TTCTGCATTT AGGGCAAAAG 6421 CCAGAGACTA CTCCACCGTT AACATGAATT ATGAATATTA TTGCGACAAG ACCCAAACAT 6481 TGATAAACCC AAATCTAGCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 6541 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 6601 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 6661 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 6721 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 6781 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 6841 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 6901 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 6961 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 7021 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 7081 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 7141 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 7201 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 7261 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 7321 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 7381 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 7441 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 7501 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 7561 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 7621 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 7681 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 7741 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 7801 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 7861 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 7921 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 7981 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 8041 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 8101 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 8161 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 8221 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 8281 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 8341 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 8401 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 8461 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 8521 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 8581 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 8641 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 8701 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 8761 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 8821 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 8881 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 8941 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1457 LOCUS ADDGENE_1457 6554 bp DNA circular SYN 01/03/2005 DEFINITION pPD81_34 complete plasmid sequence KEYWORDS . SOURCE pPD81_34 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1457 at www.addgene.org FEATURES Location/Qualifiers source 1..6554 /organism="Addgene plasmid pPD81_34" /mol_type="other DNA" CDS 26..3316 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVP SNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDG VNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMW RMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLW QGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTAD GTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDIL LMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPA MSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADT TATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAK YWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFA DRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAEN LSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQ FTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHA WQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLG LGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 36..56 /label="rf81.34" misc_feature 77..118 /label="SynIVS.A" misc_feature 152..178 /label="SV40.NLS" gene 269..3313 /label="lacZ" /gene="lacZ" promoter 4468..4496 /label="Amp_promoter" gene 4538..5398 /label="Ampicillin" /gene="Ampicillin" CDS 4538..5398 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5553..6172 /label="pBR322_origin" misc_feature 6388..6414 /label="lacI.C" promoter 6481..6510 /label="lac_promoter" misc_feature 6545..6563 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTAGAGGATC CCCGGGATTG 61 GCCAAAGGAC CCAAAGgtat gtttcgaatg atactaacat aacatagaac attttcagGA 121 GGACCCTTGG AGGGTACCGA GCTCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT 181 ACCGGTGGGT GAAGACCAGA AACAGCACCT CGAACTGAGC CGCGATATTG CCCAGCGTTT 241 CAACGCGCTG TATGGCGAGA TCGATCCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC 301 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG 361 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG 421 CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC 481 TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT 541 CTACACCAAC GTGACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC 601 GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC 661 GCGAATTATT TTTGATGGCG TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT 721 CGGTTACGGC CAGGACAGTC GTTTGCCGTC TGAATTTGAC CTGAGCGCAT TTTTACGCGC 781 CGGAGAAAAC CGCCTCGCGG TGATGGTGCT GCGCTGGAGT GACGGCAGTT ATCTGGAAGA 841 TCAGGATATG TGGCGGATGA GCGGCATTTT CCGTGACGTC TCGTTGCTGC ATAAACCGAC 901 TACACAAATC AGCGATTTCC ATGTTGCCAC TCGCTTTAAT GATGATTTCA GCCGCGCTGT 961 ACTGGAGGCT GAAGTTCAGA TGTGCGGCGA GTTGCGTGAC TACCTACGGG TAACAGTTTC 1021 TTTATGGCAG GGTGAAACGC AGGTCGCCAG CGGCACCGCG CCTTTCGGCG GTGAAATTAT 1081 CGATGAGCGT GGTGGTTATG CCGATCGCGT CACACTACGT CTGAACGTCG AAAACCCGAA 1141 ACTGTGGAGC GCCGAAATCC CGAATCTCTA TCGTGCGGTG GTTGAACTGC ACACCGCCGA 1201 CGGCACGCTG ATTGAAGCAG AAGCCTGCGA TGTCGGTTTC CGCGAGGTGC GGATTGAAAA 1261 TGGTCTGCTG CTGCTGAACG GCAAGCCGTT GCTGATTCGA GGCGTTAACC GTCACGAGCA 1321 TCATCCTCTG CATGGTCAGG TCATGGATGA GCAGACGATG GTGCAGGATA TCCTGCTGAT 1381 GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA 1441 CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG 1501 CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG 1561 CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG 1621 GAATGAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT CGCTGGATCA AATCTGTCGA 1681 TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC ACCACGGCCA CCGATATTAT 1741 TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC CCGGCTGTGC CGAAATGGTC 1801 CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG CTGATCCTTT GCGAATACGC 1861 CCACGCGATG GGTAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA 1921 TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA 1981 TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG 2041 CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG CGCTGACGGA 2101 AGCAAAACAC CAGCAGCAGT TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC 2161 CAGCGAATAC CTGTTCCGTC ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA 2221 TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT 2281 GATTGAACTG CCTGAACTAC CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG 2341 CGTAGTGCAA CCGAACGCGA CCGCATGGTC AGAAGCCGGG CACATCAGCG CCTGGCAGCA 2401 GTGGCGTCTG GCGGAAAACC TCAGTGTGAC GCTCCCCGCC GCGTCCCACG CCATCCCGCA 2461 TCTGACCACC AGCGAAATGG ATTTTTGCAT CGAGCTGGGT AATAAGCGTT GGCAATTTAA 2521 CCGCCAGTCA GGCTTTCTTT CACAGATGTG GATTGGCGAT AAAAAACAAC TGCTGACGCC 2581 GCTGCGCGAT CAGTTCACCC GTGCACCGCT GGATAACGAC ATTGGCGTAA GTGAAGCGAC 2641 CCGCATTGAC CCTAACGCCT GGGTCGAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA 2701 AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC 2761 TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA 2821 TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC 2881 GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG 2941 ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA 3001 TCTGCCATTG TCAGACATGT ATACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG 3061 CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT 3121 CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA 3181 AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG 3241 GAGCCCGTCA GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 3301 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGATCGC CATCTCGCGC CCGTGCCTCT 3361 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 3421 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 3481 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 3541 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 3601 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 3661 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 3721 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 3781 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 3841 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 3901 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 3961 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 4021 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 4081 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4141 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4201 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4261 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4321 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4381 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4441 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4501 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 4561 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4621 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 4681 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 4741 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 4801 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 4861 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 4921 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 4981 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5041 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5101 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5161 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5221 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5281 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5341 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5401 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 5461 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5521 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 5581 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 5641 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5701 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 5761 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 5821 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 5881 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 5941 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6001 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6061 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6121 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6181 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6241 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6301 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6361 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6421 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 6481 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 6541 CACAGGAAAC AGCT // 1458 LOCUS ADDGENE_1458 6779 bp DNA circular SYN 01/03/2005 DEFINITION pPD87_18 complete plasmid sequence KEYWORDS . SOURCE pPD87_18 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1458 at www.addgene.org FEATURES Location/Qualifiers source 1..6779 /organism="Addgene plasmid pPD87_18" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..3541 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 377..403 /label="SV40.NLS" gene 494..3538 /label="lacZ" /gene="lacZ" promoter 4693..4721 /label="Amp_promoter" CDS 4763..5623 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4763..5623 /label="Ampicillin" /gene="Ampicillin" rep_origin 5778..6397 /label="pBR322_origin" misc_feature 6613..6639 /label="lacI.C" promoter 6706..6735 /label="lac_promoter" misc_feature 6770..6788 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC AGAACAACTT 1621 TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG 1681 CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA 1741 TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT 1801 GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG AATCAGGCCA 1861 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 1921 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 1981 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2041 TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG CGATGGGTAA 2101 CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC GTTTACAGGG 2161 CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA ACGGCAACCC 2221 GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT TCTGTATGAA 2281 CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA AACACCAGCA 2341 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2401 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2461 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2521 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2581 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA 2641 AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA 2701 AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT 2761 TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT 2821 CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA 2881 CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG CGTTGTTGCA 2941 GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG CGTGGCAGCA 3001 TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA GTGGTCAAAT 3061 GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC GGATTGGCCT 3121 GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG GGCCGCAAGA 3181 AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC CATTGTCAGA 3241 CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT 3301 GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA 3361 ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT 3421 GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC 3481 GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA 3541 ATAGGGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA 3601 TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA 3661 TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA 3721 ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT 3781 GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC 3841 TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA 3901 AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT 3961 CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA 4021 ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT 4081 CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG 4141 ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA 4201 GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG 4261 AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT 4321 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 4381 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 4441 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 4501 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 4561 CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 4621 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 4681 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 4741 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 4801 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 4861 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 4921 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 4981 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 5041 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 5101 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 5161 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 5221 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 5281 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 5341 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 5401 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 5461 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 5521 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 5581 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 5641 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 5701 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 5761 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 5821 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 5881 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 5941 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 6001 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 6061 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 6121 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 6181 TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 6241 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 6301 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 6361 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 6421 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 6481 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 6541 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 6601 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 6661 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 6721 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1459 LOCUS ADDGENE_1459 6932 bp DNA circular SYN 01/03/2005 DEFINITION pPD87_50 complete plasmid sequence KEYWORDS . SOURCE pPD87_50 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1459 at www.addgene.org FEATURES Location/Qualifiers source 1..6932 /organism="Addgene plasmid pPD87_50" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..3694 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLC DRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGN ESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKW SIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRL TEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQ GKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAA SHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDN DIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYP DRLTAACFDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" gene 494..3691 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1595..1645 /label="SynIVS.E" misc_feature 2368..2418 /label="SynIVS.H" misc_feature 3349..3399 /label="SynIVS.K" promoter 4846..4874 /label="Amp_promoter" gene 4916..5776 /label="Ampicillin" /gene="Ampicillin" CDS 4916..5776 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5931..6550 /label="pBR322_origin" misc_feature 6766..6792 /label="lacI.C" promoter 6859..6888 /label="lac_promoter" misc_feature 6923..6941 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATgtaagt ttaaactatt cgttactaac 1621 taactttaaa catttaaatt ttcagATCCT GCTGATGAAG CAGAACAACT TTAACGCCGT 1681 GCGCTGTTCG CATTATCCGA ACCATCCGCT GTGGTACACG CTGTGCGACC GCTACGGCCT 1741 GTATGTGGTG GATGAAGCCA ATATTGAAAC CCACGGCATG GTGCCAATGA ATCGTCTGAC 1801 CGATGATCCG CGCTGGCTAC CGGCGATGAG CGAACGCGTA ACGCGAATGG TGCAGCGCGA 1861 TCGTAATCAC CCGAGTGTGA TCATCTGGTC GCTGGGGAAT GAATCAGGCC ACGGCGCTAA 1921 TCACGACGCG CTGTATCGCT GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA 1981 AGGCGGCGGA GCCGACACCA CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA 2041 TGAAGACCAG CCCTTCCCGG CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC 2101 TGGAGAGACG CGCCCGCTGA TCCTTTGCGA ATACGCCCAC GCGATGGGTA ACAGTCTTGG 2161 CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC CGTTTACAGG GCGGCTTCGT 2221 CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA AACGGCAACC CGTGGTCGGC 2281 TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG TTCTGTATGA ACGGTCTGGT 2341 CTTTGCCGAC CGCACGCCGC ATCCAGCgta agtttaaaca ataacctact aactaacgta 2401 gataatttaa attttcagGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 2461 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 2521 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 2581 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 2641 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 2701 AGCCGGGCAC ATCAGCGCCT GGCAGCAGTG GCGTCTGGCG GAAAACCTCA GTGTGACGCT 2761 CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC GAAATGGATT TTTGCATCGA 2821 GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC TTTCTTTCAC AGATGTGGAT 2881 TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG TTCACCCGTG CACCGCTGGA 2941 TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT AACGCCTGGG TCGAACGCTG 3001 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3061 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3121 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3181 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 3241 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 3301 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 3361 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 3421 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 3481 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 3541 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 3601 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 3661 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGATCGCCA 3721 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 3781 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 3841 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 3901 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 3961 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4021 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4081 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4141 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4201 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4261 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4321 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4381 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 4441 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 4501 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 4561 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 4621 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 4681 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 4741 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 4801 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 4861 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 4921 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 4981 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5041 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5101 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5161 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5221 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5281 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5341 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 5401 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 5461 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 5521 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 5581 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 5641 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 5701 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 5761 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 5821 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 5881 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 5941 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6001 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6061 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6121 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6181 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6241 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6301 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6361 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 6421 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 6481 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 6541 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 6601 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 6661 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 6721 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 6781 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 6841 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 6901 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1460 LOCUS ADDGENE_1460 6826 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_22 complete plasmid sequence KEYWORDS . SOURCE pPD88_22 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1460 at www.addgene.org FEATURES Location/Qualifiers source 1..6826 /organism="Addgene plasmid pPD88_22" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..3541 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 377..403 /label="SV40.NLS" gene 494..3538 /label="lacZ" /gene="lacZ" misc_feature 3562..3612 /label="SynIVS.L" promoter 4740..4768 /label="Amp_promoter" gene 4810..5670 /label="Ampicillin" /gene="Ampicillin" CDS 4810..5670 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5825..6444 /label="pBR322_origin" misc_feature 6660..6686 /label="lacI.C" promoter 6753..6782 /label="lac_promoter" misc_feature 6817..6835 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC AGAACAACTT 1621 TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG 1681 CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA 1741 TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT 1801 GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG AATCAGGCCA 1861 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 1921 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 1981 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2041 TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG CGATGGGTAA 2101 CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC GTTTACAGGG 2161 CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA ACGGCAACCC 2221 GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT TCTGTATGAA 2281 CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA AACACCAGCA 2341 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2401 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2461 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2521 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2581 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA 2641 AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA 2701 AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT 2761 TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT 2821 CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA 2881 CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG CGTTGTTGCA 2941 GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG CGTGGCAGCA 3001 TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA GTGGTCAAAT 3061 GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC GGATTGGCCT 3121 GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG GGCCGCAAGA 3181 AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC CATTGTCAGA 3241 CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT 3301 GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA 3361 ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT 3421 GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC 3481 GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA 3541 ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat 3601 ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA 3661 TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT 3721 TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA 3781 GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC 3841 ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT 3901 TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA 3961 TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT 4021 GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA 4081 GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG 4141 TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC 4201 AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA 4261 ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA 4321 TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT 4381 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 4441 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 4501 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 4561 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC 4621 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 4681 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 4741 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 4801 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 4861 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 4921 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 4981 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 5041 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 5101 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 5161 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 5221 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 5281 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 5341 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 5401 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 5461 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 5521 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 5581 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 5641 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 5701 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 5761 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 5821 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 5881 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 5941 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 6001 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 6061 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 6121 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 6181 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 6241 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 6301 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 6361 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 6421 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 6481 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 6541 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 6601 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 6661 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 6721 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 6781 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1461 LOCUS ADDGENE_1461 7131 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_27 complete plasmid sequence KEYWORDS . SOURCE pPD88_27 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1461 at www.addgene.org FEATURES Location/Qualifiers source 1..7131 /organism="Addgene plasmid pPD88_27" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1039 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLN LT" gene 494..3843 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 1078..3846 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1645..1695 /label="SynIVS.E" misc_feature 1952..2002 /label="SynIVS.F" misc_feature 2469..2519 /label="SynIVS.H" misc_feature 3095..3145 /label="SynIVS.J" misc_feature 3501..3551 /label="SynIVS.K" misc_feature 3867..3917 /label="SynIVS.L" promoter 5045..5073 /label="Amp_promoter" CDS 5115..5975 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5115..5975 /label="Ampicillin" /gene="Ampicillin" rep_origin 6130..6749 /label="pBR322_origin" misc_feature 6965..6991 /label="lacI.C" promoter 7058..7087 /label="lac_promoter" misc_feature 7122..7140 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt 961 aagtttaatt aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG 1021 CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG 1081 GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC 1141 ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT 1201 GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT TCAGATGTGC 1261 GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA AACGCAGGTC 1321 GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG TTATGCCGAT 1381 CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA AATCCCGAAT 1441 CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA AGCAGAAGCC 1501 TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT GAACGGCAAG 1561 CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG TCAGGTCATG 1621 GATGAGCAGA CGATGGTGCA GGATgtaagt ttaaactatt cgttactaac taactttaaa 1681 catttaaatt ttcagATCCT GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG 1741 CATTATCCGA ACCATCCGCT GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG 1801 GATGAAGCCA ATATTGAAAC CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG 1861 CGCTGGCTAC CGGCGATGAG CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC 1921 CCGAGTGTGA TCATCTGGTC GCTGGGGAAT Ggtaagttta aacagttgaa tactaactaa 1981 cggagatctt tgaaattttc agAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC 2041 TGGATCAAAT CTGTCGATCC TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC 2101 ACGGCCACCG ATATTATTTG CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG 2161 GCTGTGCCGA AATGGTCCAT CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG 2221 ATCCTTTGCG AATACGCCCA CGCGATGGGT AACAGTCTTG GCGGTTTCGC TAAATACTGG 2281 CAGGCGTTTC GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG 2341 TCGCTGATTA AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC 2401 GATACGCCGA ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG 2461 CATCCAGCgt aagtttaaac aataacctac taactaacgt agataattta aattttcagG 2521 CTGACGGAAG CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC 2581 GAAGTGACCA GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG 2641 GCGCTGGATG GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT 2701 AAACAGTTGA TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC 2761 ACAGTACGCG TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCC 2821 TGGCAGCAGT GGCGTCTGGC GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC 2881 ATCCCGCATC TGACCACCAG CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG 2941 CAATTTAACC GCCAGTCAGG CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG 3001 CTGACGCCGC TGCGCGATCA GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT 3061 GAAGCGACCC GCATTGACCC TAACGCCTGG GTCGgtaagt ttaaacaaag ttgtactaac 3121 taacgaagat cttgataatt ttcagAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA 3181 GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT 3241 CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT 3301 GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG 3361 GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA 3421 TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT 3481 CTGCCATTGT CAGACATGTA gtaagtttaa acttgatagt actaactaac atgtttcatt 3541 taaattttca gTACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG CGGGACGCGC 3601 GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT CAGCCGCTAC 3661 AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA AGAAGGCACA 3721 TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG GAGCCCGTCA 3781 GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA 3841 AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt 3901 aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT 3961 CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA 4021 AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT 4081 GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC 4141 CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG 4201 TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT 4261 TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT 4321 CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA 4381 ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT 4441 GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC 4501 GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT 4561 TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT 4621 CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC 4681 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 4741 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 4801 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 4861 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA 4921 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA 4981 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 5041 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 5101 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 5161 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 5221 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 5281 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG 5341 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 5401 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 5461 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 5521 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 5581 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 5641 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 5701 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 5761 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 5821 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 5881 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 5941 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 6001 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 6061 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 6121 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 6181 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 6241 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 6301 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 6361 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 6421 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 6481 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT 6541 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 6601 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 6661 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 6721 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 6781 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 6841 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 6901 GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC 6961 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA 7021 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 7081 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1462 LOCUS ADDGENE_1462 7335 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_75 complete plasmid sequence KEYWORDS . SOURCE pPD88_75 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1462 at www.addgene.org FEATURES Location/Qualifiers source 1..7335 /organism="Addgene plasmid pPD88_75" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1090 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 494..4047 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 628..678 /label="SynIVS.B" CDS 1129..4050 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1306..1356 /label="SynIVS.D" misc_feature 1747..1797 /label="SynIVS.E" misc_feature 2054..2104 /label="SynIVS.F" misc_feature 2335..2385 /label="SynIVS.G" misc_feature 2622..2672 /label="SynIVS.H" misc_feature 2988..3038 /label="SynIVS.I" misc_feature 3299..3349 /label="SynIVS.J" misc_feature 3705..3755 /label="SynIVS.K" misc_feature 4071..4121 /label="SynIVS.L" promoter 5249..5277 /label="Amp_promoter" gene 5319..6179 /label="Ampicillin" /gene="Ampicillin" CDS 5319..6179 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6334..6953 /label="pBR322_origin" misc_feature 7169..7195 /label="lacI.C" promoter 7262..7291 /label="lac_promoter" misc_feature 7326..7344 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 661 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 721 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 781 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 841 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 901 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 961 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 1021 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 1081 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 1141 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 1201 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1261 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1321 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1381 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1441 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1501 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1561 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1621 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1681 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1741 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1801 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1861 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1921 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1981 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 2041 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 2101 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 2161 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 2221 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2281 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2341 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2401 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2461 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2521 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2581 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2641 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2701 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2761 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2821 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2881 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2941 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 3001 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 3061 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 3121 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 3181 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3241 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3301 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3361 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3421 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3481 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3541 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3601 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3661 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3721 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3781 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3841 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3901 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3961 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 4021 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa 4081 actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC 4141 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 4201 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 4261 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 4321 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 4381 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 4441 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 4501 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 4561 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 4621 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 4681 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 4741 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4801 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4861 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4921 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4981 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 5041 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 5101 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 5161 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 5221 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 5281 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 5341 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 5401 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 5461 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 5521 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 5581 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 5641 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 5701 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 5761 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5821 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5881 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5941 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 6001 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 6061 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 6121 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 6181 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 6241 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 6301 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 6361 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 6421 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 6481 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 6541 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 6601 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 6661 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6721 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6781 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6841 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6901 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6961 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 7021 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 7081 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 7141 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 7201 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 7261 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 7321 ACACAGGAAA CAGCT // 1463 LOCUS ADDGENE_1463 7112 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_03 complete plasmid sequence KEYWORDS . SOURCE pPD89_03 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1463 at www.addgene.org FEATURES Location/Qualifiers source 1..7112 /organism="Addgene plasmid pPD89_03" /mol_type="other DNA" CDS 1..867 /label="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFA WFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENP TGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*L IFRTVVCRLNLT" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3824 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 405..455 /label="SynIVS.B" CDS 906..3827 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1083..1133 /label="SynIVS.D" misc_feature 1524..1574 /label="SynIVS.E" misc_feature 1831..1881 /label="SynIVS.F" misc_feature 2112..2162 /label="SynIVS.G" misc_feature 2399..2449 /label="SynIVS.H" misc_feature 2765..2815 /label="SynIVS.I" misc_feature 3076..3126 /label="SynIVS.J" misc_feature 3482..3532 /label="SynIVS.K" misc_feature 3848..3898 /label="SynIVS.L" promoter 5026..5054 /label="Amp_promoter" gene 5096..5956 /label="Ampicillin" /gene="Ampicillin" CDS 5096..5956 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6111..6730 /label="pBR322_origin" misc_feature 6946..6972 /label="lacI.C" promoter 7039..7068 /label="lac_promoter" misc_feature 7103..7121 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GaAGgtaagt ttaaacagat 421 ccatactaac taacttgttc tgacataatt ttcagCtTGA ATGGCGAATG GCGCTTTGCC 481 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC 541 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC 601 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT 661 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT 721 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 781 GGCCAGgtaa gtttaattaa gttgataCTA ACTAACAAGA TCTGATTAAT TTTCagGACA 841 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 901 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 961 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1021 TCCATGTTGC CACTCGCTTT AATGATGATT TCtccCGCGC TGTACTGGAG GCTGAAGTcC 1081 AGgtaagttt aaacaggatc ttactaacta acatgctaac actgaatttt cagATGTGCG 1141 GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA ACGCAGGTCG 1201 CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT TATGCCGATC 1261 GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA ATCCCGAATC 1321 TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA GCAGAAGCCT 1381 GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG AACGGCAAGC 1441 CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT CAGGTCATGG 1501 ATGAGCAGAC GATGGTGCAG GATgtaagtt taaactattc gttactaact aactttaaac 1561 atttaaattt tcagATCCTG CTGATGAAGC AGAACAACTT TAACGCCGTG CGCTGTTCGC 1621 ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG 1681 ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC 1741 GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT GCAGCGCGAT CGTAATCACC 1801 CGAGTGTGAT CATCTGGTCG CTGGGGAATG gtaagtttaa acagttgaat actaactaac 1861 ggagatcttt gaaattttca gAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT 1921 GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA 1981 CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG 2041 CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA 2101 TtCTTTGCGA Ggtaagttta aacagaactc tactaactaa cacattagat cctaattttc 2161 agTACGCtCA CGCGATGGGc AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC 2221 GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA 2281 AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA 2341 ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt 2401 aagtttaaac aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG 2461 CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA 2521 GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG 2581 GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA 2641 TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG 2701 TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCa TGGCAGCAGT 2761 GGAGgtaagt ttaaacaaga tcctactaac taactctaca ttgatgaatt ttcagACTGG 2821 CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA 2881 GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG 2941 GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC 3001 AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC 3061 CTAACGCCTG GGTCGgtaag tttaaacaaa gttgtactaa ctaacgaaga tcttgataat 3121 tttcagAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA 3181 CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG 3241 GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA 3301 TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT 3361 GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT 3421 ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT 3481 Agtaagttta aacttgatag tactaactaa catgtttcat ttaaattttc agTACCCCGT 3541 ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC 3601 ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG 3661 AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT 3721 TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC 3781 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 3841 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 3901 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 3961 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 4021 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 4081 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 4141 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4201 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4261 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4321 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4381 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4441 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4501 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4561 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 4621 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 4681 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 4741 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 4801 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 4861 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 4921 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 4981 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 5041 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 5101 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 5161 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5221 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5281 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5341 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5401 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5461 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5521 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 5581 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 5641 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 5701 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 5761 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 5821 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 5881 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 5941 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 6001 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 6061 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 6121 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6181 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6241 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6301 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6361 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6421 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6481 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6541 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 6601 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 6661 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 6721 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 6781 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 6841 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 6901 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 6961 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 7021 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 7081 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1464 LOCUS ADDGENE_1464 7111 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_09 complete plasmid sequence KEYWORDS . SOURCE pPD89_09 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1464 at www.addgene.org FEATURES Location/Qualifiers source 1..7111 /organism="Addgene plasmid pPD89_09" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..138 /label="rf22.04" CDS 153..866 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 153..179 /label="SV40.NLS" gene 270..3823 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 404..454 /label="SynIVS.B" CDS 905..3826 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1082..1132 /label="SynIVS.D" misc_feature 1523..1573 /label="SynIVS.E" misc_feature 1830..1880 /label="SynIVS.F" misc_feature 2111..2161 /label="SynIVS.G" misc_feature 2398..2448 /label="SynIVS.H" misc_feature 2764..2814 /label="SynIVS.I" misc_feature 3075..3125 /label="SynIVS.J" misc_feature 3481..3531 /label="SynIVS.K" misc_feature 3847..3897 /label="SynIVS.L" promoter 5025..5053 /label="Amp_promoter" gene 5095..5955 /label="Ampicillin" /gene="Ampicillin" CDS 5095..5955 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6110..6729 /label="pBR322_origin" misc_feature 6945..6971 /label="lacI.C" promoter 7038..7067 /label="lac_promoter" misc_feature 7102..7120 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GAGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG 181 TACCGGTGGG TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT 241 TCAACGCGCT GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 301 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 361 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG aAGgtaagtt taaacagatc 421 catactaact aacttgttct gacataattt tcagCtTGAA TGGCGAATGG CGCTTTGCCT 481 GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG 541 ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA 601 ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT 661 GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA 721 TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG GTCGGTTACG 781 GCCAGgtaag tttaattaag ttgataCTAA CTAACAAGAT CTGATTAATT TTCagGACAG 841 TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC 901 GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT 961 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT 1021 CCATGTTGCC ACTCGCTTTA ATGATGATTT CtccCGCGCT GTACTGGAGG CTGAAGTcCA 1081 Ggtaagttta aacaggatct tactaactaa catgctaaca ctgaattttc agATGTGCGG 1141 CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG CAGGGTGAAA CGCAGGTCGC 1201 CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG CGTGGTGGTT ATGCCGATCG 1261 CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG AGCGCCGAAA TCCCGAATCT 1321 CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG CTGATTGAAG CAGAAGCCTG 1381 CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG CTGCTGCTGA ACGGCAAGCC 1441 GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT CTGCATGGTC AGGTCATGGA 1501 TGAGCAGACG ATGGTGCAGG ATgtaagttt aaactattcg ttactaacta actttaaaca 1561 tttaaatttt cagATCCTGC TGATGAAGCA GAACAACTTT AACGCCGTGC GCTGTTCGCA 1621 TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC TACGGCCTGT ATGTGGTGGA 1681 TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT CGTCTGACCG ATGATCCGCG 1741 CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG CAGCGCGATC GTAATCACCC 1801 GAGTGTGATC ATCTGGTCGC TGGGGAATGg taagtttaaa cagttgaata ctaactaacg 1861 gagatctttg aaattttcag AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG 1921 GATCAAATCT GTCGATCCTT CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC 1981 GGCCACCGAT ATTATTTGCC CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC 2041 TGTGCCGAAA TGGTCCATCA AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT 2101 tCTTTGCGAG gtaagtttaa acagaactct actaactaac acattagatc ctaattttca 2161 gTACGCtCAC GCGATGGGcA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 2221 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 2281 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2341 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCgta 2401 agtttaaaca ataacctact aactaacgta gataatttaa attttcagGC TGACGGAAGC 2461 AAAACACCAG CAGCAGTTTT TCCAGTTCCG TTTATCCGGG CAAACCATCG AAGTGACCAG 2521 CGAATACCTG TTCCGTCATA GCGATAACGA GCTCCTGCAC TGGATGGTGG CGCTGGATGG 2581 TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT GGATGTCGCT CCACAAGGTA AACAGTTGAT 2641 TGAACTGCCT GAACTACCGC AGCCGGAGAG CGCCGGGCAA CTCTGGCTCA CAGTACGCGT 2701 AGTGCAACCG AACGCGACCG CATGGTCAGA AGCCGGGCAC ATCAGCGCaT GGCAGCAGTG 2761 GAGgtaagtt taaacaagat cctactaact aactctacat tgatgaattt tcagACTGGC 2821 GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC ATCCCGCATC TGACCACCAG 2881 CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG CAATTTAACC GCCAGTCAGG 2941 CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG CTGACGCCGC TGCGCGATCA 3001 GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT GAAGCGACCC GCATTGACCC 3061 TAACGCCTGG GTCGgtaagt ttaaacaaag ttgtactaac taacgaagat cttgataatt 3121 ttcagAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA GCAGCGTTGT TGCAGTGCAC 3181 GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT CACGCGTGGC AGCATCAGGG 3241 GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT GGTAGTGGTC AAATGGCGAT 3301 TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG GCGCGGATTG GCCTGAACTG 3361 CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA TTAGGGCCGC AAGAAAACTA 3421 TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT CTGCCATTGT CAGACATGTA 3481 gtaagtttaa acttgatagt actaactaac atgtttcatt taaattttca gTACCCCGTA 3541 CGTCTTCCCG AGCGAAAACG GTCTGCGCTG CGGGACGCGC GAATTGAATT ATGGCCCACA 3601 CCAGTGGCGC GGCGACTTCC AGTTCAACAT CAGCCGCTAC AGTCAACAGC AACTGATGGA 3661 AACCAGCCAT CGCCATCTGC TGCACGCGGA AGAAGGCACA TGGCTGAATA TCGACGGTTT 3721 CCATATGGGG ATTGGTGGCG ACGACTCCTG GAGCCCGTCA GTATCGGCGG AATTCCAACT 3781 GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG GGCCGCTGTC 3841 ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagCAT 3901 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 3961 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 4021 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 4081 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 4141 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 4201 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 4261 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 4321 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 4381 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 4441 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 4501 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 4561 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 4621 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 4681 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 4741 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 4801 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 4861 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 4921 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 4981 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 5041 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 5101 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 5161 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 5221 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 5281 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 5341 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 5401 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 5461 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 5521 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 5581 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 5641 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 5701 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 5761 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 5821 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 5881 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 5941 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 6001 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 6061 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 6121 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 6181 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 6241 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 6301 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 6361 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 6421 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 6481 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 6541 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 6601 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 6661 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 6721 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 6781 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 6841 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 6901 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 6961 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 7021 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 7081 AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1465 LOCUS ADDGENE_1465 7116 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_17 complete plasmid sequence KEYWORDS . SOURCE pPD89_17 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1465 at www.addgene.org FEATURES Location/Qualifiers source 1..7116 /organism="Addgene plasmid pPD89_17" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..139 /label="rf22.11" misc_feature 158..184 /label="SV40.NLS" CDS 158..871 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 275..3828 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 409..459 /label="SynIVS.B" CDS 910..3831 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1087..1137 /label="SynIVS.D" misc_feature 1528..1578 /label="SynIVS.E" misc_feature 1835..1885 /label="SynIVS.F" misc_feature 2116..2166 /label="SynIVS.G" misc_feature 2403..2453 /label="SynIVS.H" misc_feature 2769..2819 /label="SynIVS.I" misc_feature 3080..3130 /label="SynIVS.J" misc_feature 3486..3536 /label="SynIVS.K" misc_feature 3852..3902 /label="SynIVS.L" promoter 5030..5058 /label="Amp_promoter" gene 5100..5960 /label="Ampicillin" /gene="Ampicillin" CDS 5100..5960 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6115..6734 /label="pBR322_origin" misc_feature 6950..6976 /label="lacI.C" promoter 7043..7072 /label="lac_promoter" misc_feature 7107..7125 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 181 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 241 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 301 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 361 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGaAGgt aagtttaaac 421 agatccatac taactaactt gttctgacat aattttcagC tTGAATGGCG AATGGCGCTT 481 TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA 541 GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA 601 CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC 661 GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG 721 AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG TGCAACGGGC GCTGGGTCGG 781 TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC AAGATCTGAT TAATTTTCag 841 GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC 901 CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG 961 CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC 1021 GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC GCGCTGTACT GGAGGCTGAA 1081 GTcCAGgtaa gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagATG 1141 TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG TGAAACGCAG 1201 GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG ATGAGCGTGG TGGTTATGCC 1261 GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC TGTGGAGCGC CGAAATCCCG 1321 AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG GCACGCTGAT TGAAGCAGAA 1381 GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG GTCTGCTGCT GCTGAACGGC 1441 AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC ATCCTCTGCA TGGTCAGGTC 1501 ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact attcgttact aactaacttt 1561 aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA ACTTTAACGC CGTGCGCTGT 1621 TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG ACCGCTACGG CCTGTATGTG 1681 GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA TGAATCGTCT GACCGATGAT 1741 CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA TGGTGCAGCG CGATCGTAAT 1801 CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt ttaaacagtt gaatactaac 1861 taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT 1921 CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC 1981 ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC 2041 CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG 2101 CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa ctaacacatt agatcctaat 2161 tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG 2221 TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG 2281 ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG 2341 CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA 2401 GCgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt cagGCTGACG 2461 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2521 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2581 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2641 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2701 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCaTGGCAG 2761 CAGTGGAGgt aagtttaaac aagatcctac taactaactc tacattgatg aattttcagA 2821 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC 2881 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 2941 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC 3001 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT 3061 GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta ctaactaacg aagatcttga 3121 taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 3181 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 3241 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 3301 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3361 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3421 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3481 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3541 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3601 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 3661 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 3721 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 3781 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 3841 CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc 3901 agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG 3961 CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT 4021 TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA 4081 TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA 4141 ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT 4201 AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT 4261 TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT 4321 CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT 4381 ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC 4441 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC 4501 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT 4561 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC 4621 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 4681 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 4741 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 4801 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 4861 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 4921 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 4981 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5041 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5101 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5161 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5221 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5281 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5341 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5401 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5461 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5521 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5581 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 5641 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 5701 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 5761 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 5821 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 5881 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 5941 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6001 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6061 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6121 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6181 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6241 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6301 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6361 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6421 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6481 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6541 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6601 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 6661 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 6721 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 6781 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 6841 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 6901 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 6961 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7021 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7081 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1466 LOCUS ADDGENE_1466 7110 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_20 complete plasmid sequence KEYWORDS . SOURCE pPD89_20 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1466 at www.addgene.org FEATURES Location/Qualifiers source 1..7110 /organism="Addgene plasmid pPD89_20" /mol_type="other DNA" CDS 26..865 /label="Orf1" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVP ESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFN VDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRL NLT" misc_feature 36..56 /label="rf81.34" misc_feature 77..118 /label="SynIVS.A" misc_feature 152..178 /label="SV40.NLS" gene 269..3822 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 403..453 /label="SynIVS.B" CDS 904..3825 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1081..1131 /label="SynIVS.D" misc_feature 1522..1572 /label="SynIVS.E" misc_feature 1829..1879 /label="SynIVS.F" misc_feature 2110..2160 /label="SynIVS.G" misc_feature 2397..2447 /label="SynIVS.H" misc_feature 2763..2813 /label="SynIVS.I" misc_feature 3074..3124 /label="SynIVS.J" misc_feature 3480..3530 /label="SynIVS.K" misc_feature 3846..3896 /label="SynIVS.L" promoter 5024..5052 /label="Amp_promoter" gene 5094..5954 /label="Ampicillin" /gene="Ampicillin" CDS 5094..5954 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6109..6728 /label="pBR322_origin" misc_feature 6944..6970 /label="lacI.C" promoter 7037..7066 /label="lac_promoter" misc_feature 7101..7119 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTAGAGGATC CCCGGGATTG 61 GCCAAAGGAC CCAAAGgtat gtttcgaatg atactaacat aacatagaac attttcagGA 121 GGACCCTTGG AGGGTACCGA GCTCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT 181 ACCGGTGGGT GAAGACCAGA AACAGCACCT CGAACTGAGC CGCGATATTG CCCAGCGTTT 241 CAACGCGCTG TATGGCGAGA TCGATCCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC 301 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG 361 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGa AGgtaagttt aaacagatcc 421 atactaacta acttgttctg acataatttt cagCtTGAAT GGCGAATGGC GCTTTGCCTG 481 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 541 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 601 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 661 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 721 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 781 CCAGgtaagt ttaattaagt tgataCTAAC TAACAAGATC TGATTAATTT TCagGACAGT 841 CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA CCGCCTCGCG 901 GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT GTGGCGGATG 961 AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT CAGCGATTTC 1021 CATGTTGCCA CTCGCTTTAA TGATGATTTC tccCGCGCTG TACTGGAGGC TGAAGTcCAG 1081 gtaagtttaa acaggatctt actaactaac atgctaacac tgaattttca gATGTGCGGC 1141 GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC 1201 AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC 1261 GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT CCCGAATCTC 1321 TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC AGAAGCCTGC 1381 GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA CGGCAAGCCG 1441 TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT 1501 GAGCAGACGA TGGTGCAGGA Tgtaagttta aactattcgt tactaactaa ctttaaacat 1561 ttaaattttc agATCCTGCT GATGAAGCAG AACAACTTTA ACGCCGTGCG CTGTTCGCAT 1621 TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT ACGGCCTGTA TGTGGTGGAT 1681 GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC GTCTGACCGA TGATCCGCGC 1741 TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC AGCGCGATCG TAATCACCCG 1801 AGTGTGATCA TCTGGTCGCT GGGGAATGgt aagtttaaac agttgaatac taactaacgg 1861 agatctttga aattttcagA ATCAGGCCAC GGCGCTAATC ACGACGCGCT GTATCGCTGG 1921 ATCAAATCTG TCGATCCTTC CCGCCCGGTG CAGTATGAAG GCGGCGGAGC CGACACCACG 1981 GCCACCGATA TTATTTGCCC GATGTACGCG CGCGTGGATG AAGACCAGCC CTTCCCGGCT 2041 GTGCCGAAAT GGTCCATCAA AAAATGGCTT TCGCTACCTG GAGAGACGCG CCCGCTGATt 2101 CTTTGCGAGg taagtttaaa cagaactcta ctaactaaca cattagatcc taattttcag 2161 TACGCtCACG CGATGGGcAA CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT 2221 CAGTATCCCC GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA 2281 TATGATGAAA ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC 2341 GATCGCCAGT TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCgtaa 2401 gtttaaacaa taacctacta actaacgtag ataatttaaa ttttcagGCT GACGGAAGCA 2461 AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA AGTGACCAGC 2521 GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC GCTGGATGGT 2581 AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA ACAGTTGATT 2641 GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC AGTACGCGTA 2701 GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCaTG GCAGCAGTGG 2761 AGgtaagttt aaacaagatc ctactaacta actctacatt gatgaatttt cagACTGGCG 2821 GAAAACCTCA GTGTGACGCT CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC 2881 GAAATGGATT TTTGCATCGA GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC 2941 TTTCTTTCAC AGATGTGGAT TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG 3001 TTCACCCGTG CACCGCTGGA TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT 3061 AACGCCTGGG TCGgtaagtt taaacaaagt tgtactaact aacgaagatc ttgataattt 3121 tcagAACGCT GGAAGGCGGC GGGCCATTAC CAGGCCGAAG CAGCGTTGTT GCAGTGCACG 3181 GCAGATACAC TTGCTGATGC GGTGCTGATT ACGACCGCTC ACGCGTGGCA GCATCAGGGG 3241 AAAACCTTAT TTATCAGCCG GAAAACCTAC CGGATTGATG GTAGTGGTCA AATGGCGATT 3301 ACCGTTGATG TTGAAGTGGC GAGCGATACA CCGCATCCGG CGCGGATTGG CCTGAACTGC 3361 CAGCTGGCGC AGGTAGCAGA GCGGGTAAAC TGGCTCGGAT TAGGGCCGCA AGAAAACTAT 3421 CCCGACCGCC TTACTGCCGC CTGTTTTGAC CGCTGGGATC TGCCATTGTC AGACATGTAg 3481 taagtttaaa cttgatagta ctaactaaca tgtttcattt aaattttcag TACCCCGTAC 3541 GTCTTCCCGA GCGAAAACGG TCTGCGCTGC GGGACGCGCG AATTGAATTA TGGCCCACAC 3601 CAGTGGCGCG GCGACTTCCA GTTCAACATC AGCCGCTACA GTCAACAGCA ACTGATGGAA 3661 ACCAGCCATC GCCATCTGCT GCACGCGGAA GAAGGCACAT GGCTGAATAT CGACGGTTTC 3721 CATATGGGGA TTGGTGGCGA CGACTCCTGG AGCCCGTCAG TATCGGCGGA ATTCCAACTG 3781 AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG GCCGCTGTCA 3841 TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagCATC 3901 TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT 3961 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG 4021 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT 4081 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA 4141 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT 4201 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT 4261 TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT 4321 CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA 4381 TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC 4441 GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA 4501 AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA 4561 GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT 4621 GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 4681 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 4741 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 4801 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 4861 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT 4921 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 4981 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 5041 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 5101 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 5161 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 5221 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 5281 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 5341 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 5401 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 5461 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 5521 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 5581 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 5641 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 5701 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 5761 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 5821 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 5881 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 5941 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 6001 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 6061 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 6121 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 6181 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 6241 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 6301 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 6361 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 6421 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 6481 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG 6541 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 6601 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 6661 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 6721 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 6781 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 6841 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 6901 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 6961 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC 7021 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA 7081 GCGGATAACA ATTTCACACA GGAAACAGCT // 1467 LOCUS ADDGENE_1467 6096 bp DNA circular SYN 01/03/2005 DEFINITION pPD8_02 complete plasmid sequence KEYWORDS . SOURCE pPD8_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1467 at www.addgene.org FEATURES Location/Qualifiers source 1..6096 /organism="Addgene plasmid pPD8_02" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 3644..3672 /label="Amp_promoter" misc_feature complement(3971..4000) /label="SupTrp.3'" misc_feature complement(4015..4044) /label="SupTrp.5'" CDS 4080..4940 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4080..4940 /label="Ampicillin" /gene="Ampicillin" rep_origin 5095..5714 /label="pBR322_origin" misc_feature 5930..5956 /label="lacI.C" promoter 6023..6052 /label="lac_promoter" misc_feature 6087..6105 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCCTACT AGTCGGCCGT ACGGGCCCTT AAGGCCGCCT GATGCGGTAT TTTCTCCTTA 3301 CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG 3361 CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT 3421 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC 3481 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT 3541 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 3601 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 3661 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATACAA TTTTCAGAAT ACGTTTTTTG 3721 TGGGCTTGGG TATATTGTTT TTAATGTTAT ACTTGCAGTC GTGAAATTTG ATTTTCAAAT 3781 TTGTAGAAAA ATCAAGAAAA TAATTGCAAC ATTCGCTTGT GTCAAAAACC AATTTCAACA 3841 AATTTTCGTG TGAGAAATAC ATTACCAGAA GGCATTTTTT CACACGATTA GCATTTTGGA 3901 CTACTTTATT AAATTTTTGC GTGTAATTTT GAATTAAATT GTATTATATT ACTACTTAAA 3961 AAACAAAAAA TTTGACCACT GAGCGGATCG AACGCCCAAC CTTTCGATCT AGAGTCGAAC 4021 GCGCTACCAT TGCGCCAAGC AGTCATGTTA TTCTCTCTTG TCATTGAAAA AGGAAGAGTA 4081 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 4141 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 4201 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 4261 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 4321 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 4381 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 4441 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 4501 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 4561 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 4621 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 4681 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 4741 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 4801 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 4861 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 4921 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 4981 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 5041 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 5101 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 5161 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 5221 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 5281 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 5341 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 5401 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 5461 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 5521 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 5581 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 5641 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 5701 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 5761 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 5821 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 5881 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 5941 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 6001 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 6061 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1468 LOCUS ADDGENE_1468 7369 bp DNA circular SYN 01/03/2005 DEFINITION pPD8_33 complete plasmid sequence KEYWORDS . SOURCE pPD8_33 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1468 at www.addgene.org FEATURES Location/Qualifiers source 1..7369 /organism="Addgene plasmid pPD8_33" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 4917..4945 /label="Amp_promoter" misc_feature complement(5244..5273) /label="SupTrp.3'" misc_feature complement(5288..5317) /label="SupTrp.5'" gene 5353..6213 /label="Ampicillin" /gene="Ampicillin" CDS 5353..6213 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6368..6987 /label="pBR322_origin" misc_feature 7203..7229 /label="lacI.C" promoter 7296..7325 /label="lac_promoter" misc_feature 7360..7378 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 3301 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 3361 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 3421 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 3481 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 3541 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 3601 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 3661 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 3721 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 3781 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 3841 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 3901 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 3961 ATACATTCCC AATATACCAA ACATAACTGT TTAAAATTAA ACATTTTTCT AAATTTTATA 4021 TGATTTCTTT TAAATTTGCA AAAATTACTT AAATTTGAAT TCCCGCGCAA ATGAGTGACT 4081 TCATTTTCTG CATTATTGTG TTTTCCGGCT ATATTAATAG GTATTTGTTT GTGTTTTTCT 4141 TTATTTTATG ATTCGAACTC CAATTTGTAA ATTTTCGAAC ATATTTCCCT AAAGAAAAAA 4201 TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA GAAAAAAATG 4261 AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC GTCACTCATC 4321 TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC GTAAAACATT 4381 TAGAAAAATT TTTTAATAGT TATAGTGGGA CTGTATTCTG TCATTTAGGG CAAAAGCCAG 4441 AGACGCTACT CCACCGTTAA CATGAATTAT GAATATTATT GCGACAAGAC CCAAACATTG 4501 ATAAACCGCA AATCTAGCCT ACTAGTCGGC CGTACGGGCC CTTAAGGCCG CCTGATGCGG 4561 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA 4621 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG 4681 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG 4741 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC 4801 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT 4861 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 4921 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA CAATTTTCAG 4981 AATACGTTTT TTGTGGGCTT GGGTATATTG TTTTTAATGT TATACTTGCA GTCGTGAAAT 5041 TTGATTTTCA AATTTGTAGA AAAATCAAGA AAATAATTGC AACATTCGCT TGTGTCAAAA 5101 ACCAATTTCA ACAAATTTTC GTGTGAGAAA TACATTACCA GAAGGCATTT TTTCACACGA 5161 TTAGCATTTT GGACTACTTT ATTAAATTTT TGCGTGTAAT TTTGAATTAA ATTGTATTAT 5221 ATTACTACTT AAAAAACAAA AAATTTGACC ACTGAGCGGA TCGAACGCCC AACCTTTCGA 5281 TCTAGAGTCG AACGCGCTAC CATTGCGCCA AGCAGTCATG TTATTCTCTC TTGTCATTGA 5341 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 5401 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 5461 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 5521 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 5581 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 5641 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5701 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5761 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5821 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5881 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5941 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 6001 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 6061 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 6121 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 6181 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 6241 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 6301 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 6361 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 6421 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 6481 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 6541 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 6601 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6661 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6721 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 6781 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6841 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6901 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6961 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 7021 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 7081 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 7141 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 7201 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 7261 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 7321 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1469 LOCUS ADDGENE_1469 6603 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_23 complete plasmid sequence KEYWORDS . SOURCE pPD90_23 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1469 at www.addgene.org FEATURES Location/Qualifiers source 1..6603 /organism="Addgene plasmid pPD90_23" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" misc_feature 3339..3389 /label="SynIVS.L" promoter 4517..4545 /label="Amp_promoter" gene 4587..5447 /label="Ampicillin" /gene="Ampicillin" CDS 4587..5447 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5602..6221 /label="pBR322_origin" misc_feature 6437..6463 /label="lacI.C" promoter 6530..6559 /label="lac_promoter" misc_feature 6594..6612 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac tgagttctac 3361 taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT 3421 CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT 3481 GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT 3541 AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA 3601 TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT 3661 ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA 3721 CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC 3781 ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT 3841 TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG 3901 GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT 3961 ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG 4021 GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG 4081 ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC 4141 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 4201 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 4261 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 4321 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 4381 CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 4441 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 4501 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 4561 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 4621 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 4681 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 4741 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 4801 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 4861 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 4921 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 4981 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 5041 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 5101 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 5161 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 5221 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 5281 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 5341 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 5401 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 5461 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 5521 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 5581 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 5641 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 5701 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 5761 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 5821 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 5881 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 5941 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 6001 AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 6061 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 6121 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 6181 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 6241 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 6301 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 6361 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 6421 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 6481 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 6541 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 6601 GCT // 1470 LOCUS ADDGENE_1470 6709 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_28 complete plasmid sequence KEYWORDS . SOURCE pPD90_28 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1470 at www.addgene.org FEATURES Location/Qualifiers source 1..6709 /organism="Addgene plasmid pPD90_28" /mol_type="other DNA" CDS 1..3471 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYV VDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGAN HDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQ QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC FDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDF QFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGR YHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3468 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1372..1422 /label="SynIVS.E" misc_feature 2145..2195 /label="SynIVS.H" misc_feature 3126..3176 /label="SynIVS.K" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA Tgtaagttta 1381 aactattcgt tactaactaa ctttaaacat ttaaattttc agATCCTGCT GATGAAGCAG 1441 AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG 1501 TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG 1561 CCAATGAATC GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG 1621 CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA 1681 TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC 1741 CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG 1801 ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA 1861 AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG 1921 ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT 1981 TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC 2041 GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC 2101 TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCgtaagt ttaaacaata 2161 acctactaac taacgtagat aatttaaatt ttcagGCTGA CGGAAGCAAA ACACCAGCAG 2221 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2281 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2341 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2401 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2461 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2521 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2581 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2641 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2701 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2761 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2821 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2881 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2941 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3001 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3061 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3121 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3181 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3241 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 3301 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 3361 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 3421 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 3481 CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1471 LOCUS ADDGENE_1471 6908 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_31 complete plasmid sequence KEYWORDS . SOURCE pPD90_31 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1471 at www.addgene.org FEATURES Location/Qualifiers source 1..6908 /organism="Addgene plasmid pPD90_31" /mol_type="other DNA" CDS 1..816 /label="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3620 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 855..3623 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1422..1472 /label="SynIVS.E" misc_feature 1729..1779 /label="SynIVS.F" misc_feature 2246..2296 /label="SynIVS.H" misc_feature 2872..2922 /label="SynIVS.J" misc_feature 3278..3328 /label="SynIVS.K" misc_feature 3644..3694 /label="SynIVS.L" promoter 4822..4850 /label="Amp_promoter" gene 4892..5752 /label="Ampicillin" /gene="Ampicillin" CDS 4892..5752 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5907..6526 /label="pBR322_origin" misc_feature 6742..6768 /label="lacI.C" promoter 6835..6864 /label="lac_promoter" misc_feature 6899..6917 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGgtaag tttaattaag ttgataCTAA CTAACAAGAT CTGATTAATT 781 TTCagGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA 841 ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA 901 TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA 961 TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT GTACTGGAGG 1021 CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC 1081 AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC 1141 GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA 1201 GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC 1261 TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC 1321 TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC 1381 TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA Tgtaagttta aactattcgt 1441 tactaactaa ctttaaacat ttaaattttc agATCCTGCT GATGAAGCAG AACAACTTTA 1501 ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT 1561 ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC 1621 GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC 1681 AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGgt aagtttaaac 1741 agttgaatac taactaacgg agatctttga aattttcagA ATCAGGCCAC GGCGCTAATC 1801 ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG CAGTATGAAG 1861 GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG CGCGTGGATG 1921 AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT TCGCTACCTG 1981 GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC AGTCTTGGCG 2041 GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC GGCTTCGTCT 2101 GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG TGGTCGGCTT 2161 ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC GGTCTGGTCT 2221 TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat aacctactaa ctaacgtaga 2281 taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA GCAGTTTTTC CAGTTCCGTT 2341 TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT CCGTCATAGC GATAACGAGC 2401 TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG 2461 ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA ACTACCGCAG CCGGAGAGCG 2521 CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA CGCGACCGCA TGGTCAGAAG 2581 CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA AAACCTCAGT GTGACGCTCC 2641 CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA AATGGATTTT TGCATCGAGC 2701 TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT TCTTTCACAG ATGTGGATTG 2761 GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT CACCCGTGCA CCGCTGGATA 2821 ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA CGCCTGGGTC Ggtaagttta 2881 aacaaagttg tactaactaa cgaagatctt gataattttc agAACGCTGG AAGGCGGCGG 2941 GCCATTACCA GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG 3001 TGCTGATTAC GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA 3061 AAACCTACCG GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA 3121 GCGATACACC GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC 3181 GGGTAAACTG GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT 3241 GTTTTGACCG CTGGGATCTG CCATTGTCAG ACATGTAgta agtttaaact tgatagtact 3301 aactaacatg tttcatttaa attttcagTA CCCCGTACGT CTTCCCGAGC GAAAACGGTC 3361 TGCGCTGCGG GACGCGCGAA TTGAATTATG GCCCACACCA GTGGCGCGGC GACTTCCAGT 3421 TCAACATCAG CCGCTACAGT CAACAGCAAC TGATGGAAAC CAGCCATCGC CATCTGCTGC 3481 ACGCGGAAGA AGGCACATGG CTGAATATCG ACGGTTTCCA TATGGGGATT GGTGGCGACG 3541 ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT TCCAACTGAG CGCCGGTCGC TACCATTACC 3601 AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt taaactgagt 3661 tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC CTCTGACTTC 3721 TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA 3781 TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA 3841 CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA 3901 AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC 3961 TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA 4021 CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC 4081 TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT 4141 ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT 4201 TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA 4261 CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT 4321 GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT 4381 AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC 4441 GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 4501 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 4561 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 4621 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 4681 CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 4741 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 4801 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 4861 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 4921 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 4981 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 5041 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 5101 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 5161 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 5221 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 5281 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 5341 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 5401 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 5461 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 5521 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 5581 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 5641 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 5701 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 5761 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 5821 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 5881 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 5941 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 6001 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 6061 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 6121 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 6181 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 6241 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 6301 CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 6361 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 6421 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 6481 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 6541 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 6601 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 6661 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 6721 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 6781 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 6841 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 6901 AAACAGCT // 1472 LOCUS ADDGENE_1472 4438 bp DNA circular SYN 01/03/2005 DEFINITION pPD91_14 complete plasmid sequence KEYWORDS . SOURCE pPD91_14 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1472 at www.addgene.org FEATURES Location/Qualifiers source 1..4438 /organism="Addgene plasmid pPD91_14" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1135 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAM PEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1126 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 602..625 /label="gfp.S65S" promoter 2352..2380 /label="Amp_promoter" CDS 2422..3282 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2422..3282 /label="Ampicillin" /gene="Ampicillin" rep_origin 3437..4056 /label="pBR322_origin" misc_feature 4272..4298 /label="lacI.C" promoter 4365..4394 /label="lac_promoter" misc_feature 4429..4447 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACACTTGT 601 CACTACTTTC TCTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCATA TGAAACgGCA 661 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 721 AGATGACGGG AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA 781 TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT 841 GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT 901 CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 961 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 1021 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 1081 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC 1141 GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 1201 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 1261 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 1321 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 1381 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 1441 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 1501 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 1561 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 1621 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 1681 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 1741 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 1801 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 1861 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 1921 ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC 1981 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG 2041 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG 2101 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG 2161 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC 2221 GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT 2281 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 2341 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 2401 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 2461 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 2521 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 2581 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 2641 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 2701 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 2761 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 2821 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 2881 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 2941 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 3001 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 3061 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 3121 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 3181 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 3241 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 3301 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 3361 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 3421 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 3481 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 3541 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC 3601 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 3661 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 3721 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 3781 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 3841 GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 3901 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 3961 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 4021 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 4081 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 4141 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 4201 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 4261 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 4321 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 4381 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1473 LOCUS ADDGENE_1473 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_48 complete plasmid sequence KEYWORDS . SOURCE pPD93_48 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1473 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD93_48" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1474 LOCUS ADDGENE_1474 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_51 complete plasmid sequence KEYWORDS . SOURCE pPD93_51 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1474 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD93_51" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CaCTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1475 LOCUS ADDGENE_1475 4693 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_65 complete plasmid sequence KEYWORDS . SOURCE pPD93_65 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1475 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid pPD93_65" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1302..1352 /label="SynIVS.M" misc_feature 1429..1479 /label="SynIVS.L" promoter 2607..2635 /label="Amp_promoter" gene 2677..3537 /label="Ampicillin" /gene="Ampicillin" CDS 2677..3537 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3692..4311 /label="pBR322_origin" misc_feature 4527..4553 /label="lacI.C" promoter 4620..4649 /label="lac_promoter" misc_feature 4684..4702 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CaCTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT Tgtaagttta aactttctca 1321 tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA 1381 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 1441 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 1501 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 1561 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 1621 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 1681 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 1741 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 1801 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 1861 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 1921 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 1981 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 2041 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 2101 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 2161 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 2221 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 2281 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 2341 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 2401 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 2461 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 2521 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 2581 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 2641 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 2701 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 2761 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 2821 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 2881 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 2941 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 3001 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 3061 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 3121 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 3181 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 3241 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 3301 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 3361 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 3421 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 3481 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 3541 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 3601 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 3661 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 3721 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 3781 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 3841 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 3901 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 3961 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 4021 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 4081 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 4141 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 4201 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 4261 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 4321 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 4381 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 4441 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 4501 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 4561 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 4621 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 4681 ACAGGAAACA GCT // 1476 LOCUS ADDGENE_1476 6787 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_97 complete plasmid sequence KEYWORDS . SOURCE pPD93_97 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1476 at www.addgene.org FEATURES Location/Qualifiers source 1..6787 /organism="Addgene plasmid pPD93_97" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2526..2552 /label="SV40.NLS" CDS 2526..3437 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 2568..3428 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2739..2789 /label="SynIVS.R" misc_feature 2811..2830 /label="gfp.S65C" misc_feature 2944..2994 /label="SynIVS.S" misc_feature 3159..3209 /label="SynIVS.T" misc_feature 3523..3573 /label="SynIVS.L" promoter 4701..4729 /label="Amp_promoter" CDS 4771..5631 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4771..5631 /label="Ampicillin" /gene="Ampicillin" rep_origin 5786..6405 /label="pBR322_origin" misc_feature 6621..6647 /label="lacI.C" promoter 6714..6743 /label="lac_promoter" misc_feature 6778..6796 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 2461 aatgatacta acataacata gaacattttc agGAGGACCC TTGGAGGGTA CCGAGCTCAG 2521 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG AGTAAAGGAG 2581 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 2641 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 2701 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac 2761 taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG 2821 TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA 2881 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 2941 CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG 3001 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 3061 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 3121 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac 3181 taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG 3241 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 3301 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 3361 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 3421 ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3481 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 3541 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 3601 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 3661 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 3721 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3781 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 3841 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 3901 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 3961 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 4021 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 4081 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 4141 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 4201 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 4261 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 4321 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 4381 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 4441 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 4501 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 4561 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 4621 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 4681 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 4741 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4801 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 4861 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 4921 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 4981 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 5041 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 5101 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 5161 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 5221 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 5281 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 5341 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 5401 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 5461 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 5521 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 5581 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 5641 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 5701 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 5761 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 5821 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 5881 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 5941 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 6001 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 6061 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 6121 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 6181 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 6241 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 6301 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 6361 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 6421 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 6481 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 6541 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 6601 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 6661 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 6721 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6781 AACAGCT // 1477 LOCUS ADDGENE_1477 4693 bp DNA circular SYN 01/03/2005 DEFINITION pPD94_81 complete plasmid sequence KEYWORDS . SOURCE pPD94_81 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1477 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid pPD94_81" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 286..305 /label="gfp.S65C" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA 301 TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1478 LOCUS ADDGENE_1478 3786 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_02 complete plasmid sequence KEYWORDS . SOURCE pPD95_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1478 at www.addgene.org FEATURES Location/Qualifiers source 1..3786 /organism="Addgene plasmid pPD95_02" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1431 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLV TTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*P YIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYL STQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPGDPLVLERPPPRWSSNSPY SESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNG EWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPL GFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 103..963 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 274..324 /label="SynIVS.R" misc_feature 346..365 /label="gfp.S65C" misc_feature 479..529 /label="SynIVS.S" misc_feature 694..744 /label="SynIVS.T" promoter complement(1027..1045) /label="T7_promoter" misc_feature 1065..1213 /label="lacZ_a" misc_feature complement(1056..1072) /label="M13f20_primer" rep_origin 1231..1537 /label="f1_origin" promoter 1730..1758 /label="Amp_promoter" gene 1800..2660 /label="Ampicillin" /gene="Ampicillin" CDS 1800..2660 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2815..3434 /label="pBR322_origin" misc_feature 3650..3676 /label="lacI.C" promoter 3743..3772 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 121 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 181 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 241 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 301 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA 361 TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC 421 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 481 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 541 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 601 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 661 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 721 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 781 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 841 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 901 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 961 CTATACAAAT GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC 1021 AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT 1081 GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC 1141 AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG 1201 AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 1261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 1321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 1381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 1441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 1501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 1561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 1621 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT 1681 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 1741 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 1801 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 1861 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 1921 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 1981 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 2041 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 2101 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 2161 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 2221 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 2281 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 2341 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 2401 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 2461 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 2521 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 2581 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 2641 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 2701 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 2761 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 2821 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 2881 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 2941 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 3001 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 3061 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 3121 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 3181 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 3241 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 3301 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 3361 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 3421 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 3481 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 3541 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 3601 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 3661 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 3721 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 3781 TTGTGA // 1479 LOCUS ADDGENE_1479 7229 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_03 complete plasmid sequence KEYWORDS . SOURCE pPD95_03 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1479 at www.addgene.org FEATURES Location/Qualifiers source 1..7229 /organism="Addgene plasmid pPD95_03" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" CDS 137..850 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 137..163 /label="SV40.NLS" gene 254..3807 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 388..438 /label="SynIVS.B" CDS 889..3810 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1066..1116 /label="SynIVS.D" misc_feature 1507..1557 /label="SynIVS.E" misc_feature 1814..1864 /label="SynIVS.F" misc_feature 2095..2145 /label="SynIVS.G" misc_feature 2382..2432 /label="SynIVS.H" misc_feature 2748..2798 /label="SynIVS.I" misc_feature 3059..3109 /label="SynIVS.J" misc_feature 3465..3515 /label="SynIVS.K" misc_feature 3831..3881 /label="SynIVS.L" promoter 5009..5037 /label="Amp_promoter" gene 5079..5939 /label="Ampicillin" /gene="Ampicillin" CDS 5079..5939 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6094..6713 /label="pBR322_origin" misc_feature 6929..6955 /label="lacI.C" promoter 7022..7051 /label="lac_promoter" misc_feature 7086..7104 /label="M13r_primer" misc_feature 7118..7168 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 181 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 241 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 301 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 361 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 421 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 481 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 541 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 601 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 661 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 721 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 781 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 841 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 901 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 961 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1021 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1081 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1141 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1201 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1261 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1321 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1381 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1441 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1501 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1561 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1621 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1681 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1741 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 1801 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 1861 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1921 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1981 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2041 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2101 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2161 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2221 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2281 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2341 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2401 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2461 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2521 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2581 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2641 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2701 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 2761 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 2821 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 2881 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 2941 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3001 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3061 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3121 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3181 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3241 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3301 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3361 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3421 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3481 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3541 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3601 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3661 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3721 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 3781 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa 3841 actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC 3901 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 3961 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 4021 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 4081 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 4141 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 4201 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 4261 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 4321 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 4381 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 4441 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 4501 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4561 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4621 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4681 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4741 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 4801 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 4861 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 4921 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 4981 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 5041 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 5101 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 5161 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 5221 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 5281 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 5341 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 5401 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 5461 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 5521 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5581 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5641 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5701 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 5761 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 5821 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 5881 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 5941 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 6001 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 6061 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 6121 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 6181 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 6241 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 6301 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 6361 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 6421 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6481 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6541 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6601 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6661 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6721 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 6781 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 6841 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 6901 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 6961 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 7021 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 7081 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact 7141 aactaactat tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA 7201 TGGATACGCT AACAACTTGG AAATGAAAT // 1480 LOCUS ADDGENE_1480 7233 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_07 complete plasmid sequence KEYWORDS . SOURCE pPD95_07 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1480 at www.addgene.org FEATURES Location/Qualifiers source 1..7233 /organism="Addgene plasmid pPD95_07" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" misc_feature 141..167 /label="SV40.NLS" CDS 141..854 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 258..3811 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 392..442 /label="SynIVS.B" CDS 893..3814 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1070..1120 /label="SynIVS.D" misc_feature 1511..1561 /label="SynIVS.E" misc_feature 1818..1868 /label="SynIVS.F" misc_feature 2099..2149 /label="SynIVS.G" misc_feature 2386..2436 /label="SynIVS.H" misc_feature 2752..2802 /label="SynIVS.I" misc_feature 3063..3113 /label="SynIVS.J" misc_feature 3469..3519 /label="SynIVS.K" misc_feature 3835..3885 /label="SynIVS.L" promoter 5013..5041 /label="Amp_promoter" CDS 5083..5943 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5083..5943 /label="Ampicillin" /gene="Ampicillin" rep_origin 6098..6717 /label="pBR322_origin" misc_feature 6933..6959 /label="lacI.C" promoter 7026..7055 /label="lac_promoter" misc_feature 7090..7108 /label="M13r_primer" misc_feature 7122..7172 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTGGGTG 181 AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC AACGCGCTGT 241 ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 301 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 361 GCACCGATCG CCCTTCCCAA CAGTTGCGaA Ggtaagttta aacagatcca tactaactaa 421 cttgttctga cataattttc agCtTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC 481 CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG 541 TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT CTACACCAAC GTGACCTATC 601 CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA 661 CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG 721 TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT CGGTTACGGC CAGgtaagtt 781 taattaagtt gataCTAACT AACAAGATCT GATTAATTTT CagGACAGTC GTTTGCCGTC 841 TGAATTTGAC CTGAGCGCAT TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT 901 GCGCTGGAGT GACGGCAGTT ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT 961 CCGTGACGTC TCGTTGCTGC ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC 1021 TCGCTTTAAT GATGATTTCt ccCGCGCTGT ACTGGAGGCT GAAGTcCAGg taagtttaaa 1081 caggatctta ctaactaaca tgctaacact gaattttcag ATGTGCGGCG AGTTGCGTGA 1141 CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC 1201 GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG 1261 TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT 1321 GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT 1381 CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG 1441 AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT 1501 GGTGCAGGAT gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca 1561 gATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA 1621 TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT 1681 TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC 1741 GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT 1801 CTGGTCGCTG GGGAATGgta agtttaaaca gttgaatact aactaacgga gatctttgaa 1861 attttcagAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT 1921 CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT 1981 TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG 2041 GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATtC TTTGCGAGgt 2101 aagtttaaac agaactctac taactaacac attagatcct aattttcagT ACGCtCACGC 2161 GATGGGcAAC AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG 2221 TTTACAGGGC GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA 2281 CGGCAACCCG TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT 2341 CTGTATGAAC GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat 2401 aacctactaa ctaacgtaga taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA 2461 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2521 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2581 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2641 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2701 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCaTGG CAGCAGTGGA Ggtaagttta 2761 aacaagatcc tactaactaa ctctacattg atgaattttc agACTGGCGG AAAACCTCAG 2821 TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG ACCACCAGCG AAATGGATTT 2881 TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC CAGTCAGGCT TTCTTTCACA 2941 GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG CGCGATCAGT TCACCCGTGC 3001 ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC ATTGACCCTA ACGCCTGGGT 3061 CGgtaagttt aaacaaagtt gtactaacta acgaagatct tgataatttt cagAACGCTG 3121 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3181 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3241 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3301 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 3361 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 3421 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 3481 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 3541 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 3601 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 3661 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 3721 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 3781 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt 3841 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG 3901 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 3961 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 4021 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 4081 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 4141 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT 4201 CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA 4261 ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA 4321 AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT 4381 GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT 4441 TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT 4501 TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG 4561 GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA 4621 GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 4681 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 4741 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 4801 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 4861 GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT 4921 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 4981 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 5041 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 5101 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 5161 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 5221 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 5281 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 5341 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 5401 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 5461 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 5521 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 5581 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 5641 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 5701 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 5761 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 5821 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 5881 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 5941 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 6001 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 6061 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 6121 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 6181 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 6241 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 6301 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 6361 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 6421 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 6481 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 6541 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 6601 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 6661 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 6721 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 6781 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC 6841 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA 6901 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC 6961 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC 7021 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 7081 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct 7141 tactaactaa ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA 7201 ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1481 LOCUS ADDGENE_1481 7227 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_10 complete plasmid sequence KEYWORDS . SOURCE pPD95_10 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1481 at www.addgene.org FEATURES Location/Qualifiers source 1..7227 /organism="Addgene plasmid pPD95_10" /mol_type="other DNA" CDS 9..848 /label="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVP ESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFN VDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRL NLT" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" gene 252..3805 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 386..436 /label="SynIVS.B" CDS 887..3808 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1064..1114 /label="SynIVS.D" misc_feature 1505..1555 /label="SynIVS.E" misc_feature 1812..1862 /label="SynIVS.F" misc_feature 2093..2143 /label="SynIVS.G" misc_feature 2380..2430 /label="SynIVS.H" misc_feature 2746..2796 /label="SynIVS.I" misc_feature 3057..3107 /label="SynIVS.J" misc_feature 3463..3513 /label="SynIVS.K" misc_feature 3829..3879 /label="SynIVS.L" promoter 5007..5035 /label="Amp_promoter" gene 5077..5937 /label="Ampicillin" /gene="Ampicillin" CDS 5077..5937 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6092..6711 /label="pBR322_origin" misc_feature 6927..6953 /label="lacI.C" promoter 7020..7049 /label="lac_promoter" misc_feature 7084..7102 /label="M13r_primer" misc_feature 7116..7166 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTG GGTGAAGACC 181 AGAAACAGCA CCTCGAACTG AGCCGCGATA TTGCCCAGCG TTTCAACGCG CTGTATGGCG 241 AGATCGATCC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 301 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 361 ATCGCCCTTC CCAACAGTTG CGaAGgtaag tttaaacaga tccatactaa ctaacttgtt 421 ctgacataat tttcagCtTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG GCACCAGAAG 481 CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC GTCGTCCCCT 541 CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC TATCCCATTA 601 CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG CTCACATTTA 661 ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT GGCGTTAACT 721 CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGgta agtttaatta 781 agttgataCT AACTAACAAG ATCTGATTAA TTTTCagGAC AGTCGTTTGC CGTCTGAATT 841 TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC GCGGTGATGG TGCTGCGCTG 901 GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG ATGAGCGGCA TTTTCCGTGA 961 CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT TTCCATGTTG CCACTCGCTT 1021 TAATGATGAT TTCtccCGCG CTGTACTGGA GGCTGAAGTc CAGgtaagtt taaacaggat 1081 cttactaact aacatgctaa cactgaattt tcagATGTGC GGCGAGTTGC GTGACTACCT 1141 ACGGGTAACA GTTTCTTTAT GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT 1201 CGGCGGTGAA ATTATCGATG AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA 1261 CGTCGAAAAC CCGAAACTGT GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA 1321 ACTGCACACC GCCGACGGCA CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA 1381 GGTGCGGATT GAAAATGGTC TGCTGCTGCT GAACGGCAAG CCGTTGCTGA TTCGAGGCGT 1441 TAACCGTCAC GAGCATCATC CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA 1501 GGATgtaagt ttaaactatt cgttactaac taactttaaa catttaaatt ttcagATCCT 1561 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1621 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1681 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1741 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1801 GCTGGGGAAT Ggtaagttta aacagttgaa tactaactaa cggagatctt tgaaattttc 1861 agAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC TGGATCAAAT CTGTCGATCC 1921 TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC ACGGCCACCG ATATTATTTG 1981 CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG GCTGTGCCGA AATGGTCCAT 2041 CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG ATtCTTTGCG AGgtaagttt 2101 aaacagaact ctactaacta acacattaga tcctaatttt cagTACGCtC ACGCGATGGG 2161 cAACAGTCTT GGCGGTTTCG CTAAATACTG GCAGGCGTTT CGTCAGTATC CCCGTTTACA 2221 GGGCGGCTTC GTCTGGGACT GGGTGGATCA GTCGCTGATT AAATATGATG AAAACGGCAA 2281 CCCGTGGTCG GCTTACGGCG GTGATTTTGG CGATACGCCG AACGATCGCC AGTTCTGTAT 2341 GAACGGTCTG GTCTTTGCCG ACCGCACGCC GCATCCAGCg taagtttaaa caataaccta 2401 ctaactaacg tagataattt aaattttcag GCTGACGGAA GCAAAACACC AGCAGCAGTT 2461 TTTCCAGTTC CGTTTATCCG GGCAAACCAT CGAAGTGACC AGCGAATACC TGTTCCGTCA 2521 TAGCGATAAC GAGCTCCTGC ACTGGATGGT GGCGCTGGAT GGTAAGCCGC TGGCAAGCGG 2581 TGAAGTGCCT CTGGATGTCG CTCCACAAGG TAAACAGTTG ATTGAACTGC CTGAACTACC 2641 GCAGCCGGAG AGCGCCGGGC AACTCTGGCT CACAGTACGC GTAGTGCAAC CGAACGCGAC 2701 CGCATGGTCA GAAGCCGGGC ACATCAGCGC aTGGCAGCAG TGGAGgtaag tttaaacaag 2761 atcctactaa ctaactctac attgatgaat tttcagACTG GCGGAAAACC TCAGTGTGAC 2821 GCTCCCCGCC GCGTCCCACG CCATCCCGCA TCTGACCACC AGCGAAATGG ATTTTTGCAT 2881 CGAGCTGGGT AATAAGCGTT GGCAATTTAA CCGCCAGTCA GGCTTTCTTT CACAGATGTG 2941 GATTGGCGAT AAAAAACAAC TGCTGACGCC GCTGCGCGAT CAGTTCACCC GTGCACCGCT 3001 GGATAACGAC ATTGGCGTAA GTGAAGCGAC CCGCATTGAC CCTAACGCCT GGGTCGgtaa 3061 gtttaaacaa agttgtacta actaacgaag atcttgataa ttttcagAAC GCTGGAAGGC 3121 GGCGGGCCAT TACCAGGCCG AAGCAGCGTT GTTGCAGTGC ACGGCAGATA CACTTGCTGA 3181 TGCGGTGCTG ATTACGACCG CTCACGCGTG GCAGCATCAG GGGAAAACCT TATTTATCAG 3241 CCGGAAAACC TACCGGATTG ATGGTAGTGG TCAAATGGCG ATTACCGTTG ATGTTGAAGT 3301 GGCGAGCGAT ACACCGCATC CGGCGCGGAT TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC 3361 AGAGCGGGTA AACTGGCTCG GATTAGGGCC GCAAGAAAAC TATCCCGACC GCCTTACTGC 3421 CGCCTGTTTT GACCGCTGGG ATCTGCCATT GTCAGACATG TAgtaagttt aaacttgata 3481 gtactaacta acatgtttca tttaaatttt cagTACCCCG TACGTCTTCC CGAGCGAAAA 3541 CGGTCTGCGC TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT 3601 CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT 3661 GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG 3721 CGACGACTCC TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA 3781 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 3841 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 3901 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 3961 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 4021 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 4081 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 4141 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 4201 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 4261 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 4321 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 4381 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 4441 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 4501 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 4561 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 4621 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 4681 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 4741 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 4801 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 4861 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 4921 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 4981 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 5041 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 5101 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 5161 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 5221 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 5281 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 5341 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 5401 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 5461 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 5521 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 5581 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 5641 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 5701 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 5761 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 5821 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 5881 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 5941 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 6001 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 6061 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 6121 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 6181 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 6241 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 6301 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 6361 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 6421 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 6481 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 6541 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 6601 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 6661 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 6721 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 6781 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 6841 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 6901 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 6961 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 7021 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 7081 ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa 7141 ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG 7201 GATACGCTAA CAACTTGGAA ATGAAAT // 1482 LOCUS ADDGENE_1482 6434 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_11 complete plasmid sequence KEYWORDS . SOURCE pPD95_11 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1482 at www.addgene.org FEATURES Location/Qualifiers source 1..6434 /organism="Addgene plasmid pPD95_11" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..850 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 254..3807 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 388..438 /label="SynIVS.B" CDS 889..3810 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1066..1116 /label="SynIVS.D" misc_feature 1507..1557 /label="SynIVS.E" misc_feature 1814..1864 /label="SynIVS.F" misc_feature 2095..2145 /label="SynIVS.G" misc_feature 2382..2432 /label="SynIVS.H" misc_feature 2748..2798 /label="SynIVS.I" misc_feature 3059..3109 /label="SynIVS.J" misc_feature 3465..3515 /label="SynIVS.K" promoter 4214..4242 /label="Amp_promoter" CDS 4284..5144 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4284..5144 /label="Ampicillin" /gene="Ampicillin" rep_origin 5299..5918 /label="pBR322_origin" misc_feature 6134..6160 /label="lacI.C" promoter 6227..6256 /label="lac_promoter" misc_feature 6291..6309 /label="M13r_primer" misc_feature 6323..6373 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 181 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 241 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 301 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 361 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 421 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 481 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 541 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 601 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 661 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 721 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 781 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 841 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 901 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 961 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1021 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1081 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1141 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1201 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1261 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1321 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1381 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1441 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1501 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1561 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1621 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1681 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1741 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 1801 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 1861 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1921 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1981 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2041 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2101 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2161 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2221 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2281 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2341 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2401 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2461 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2521 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2581 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2641 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2701 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 2761 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 2821 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 2881 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 2941 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3001 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3061 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3121 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3181 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3241 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3301 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3361 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3421 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3481 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3541 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3601 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3661 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3721 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 3781 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGCCTACT AGTCGGCCGT ACGGGCCCTT 3841 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 3901 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 3961 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4021 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4081 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4141 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4201 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4261 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4321 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4381 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4441 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4501 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 4561 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 4621 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 4681 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 4741 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 4801 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 4861 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 4921 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 4981 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5041 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5101 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5161 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5221 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5281 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5341 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5401 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5461 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 5521 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 5581 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 5641 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 5701 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 5761 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 5821 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 5881 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 5941 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6001 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6061 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6121 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6181 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6241 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6301 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6361 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6421 CTTGGAAATG AAAT // 1483 LOCUS ADDGENE_1483 8838 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_16 complete plasmid sequence KEYWORDS . SOURCE pPD95_16 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1483 at www.addgene.org FEATURES Location/Qualifiers source 1..8838 /organism="Addgene plasmid pPD95_16" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" CDS 1746..2459 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 1746..1772 /label="SV40.NLS" gene 1863..5416 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1997..2047 /label="SynIVS.B" CDS 2498..5419 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2675..2725 /label="SynIVS.D" misc_feature 3116..3166 /label="SynIVS.E" misc_feature 3423..3473 /label="SynIVS.F" misc_feature 3704..3754 /label="SynIVS.G" misc_feature 3991..4041 /label="SynIVS.H" misc_feature 4357..4407 /label="SynIVS.I" misc_feature 4668..4718 /label="SynIVS.J" misc_feature 5074..5124 /label="SynIVS.K" misc_feature 5440..5490 /label="SynIVS.L" promoter 6618..6646 /label="Amp_promoter" gene 6688..7548 /label="Ampicillin" /gene="Ampicillin" CDS 6688..7548 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7703..8322 /label="pBR322_origin" misc_feature 8538..8564 /label="lacI.C" promoter 8631..8660 /label="lac_promoter" misc_feature 8695..8713 /label="M13r_primer" misc_feature 8727..8777 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC CAGAAACAGC 1801 ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC GAGATCGATC 1861 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 1921 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 1981 CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt tctgacataa 2041 ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG 2101 AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC 2161 AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC 2221 CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG 2281 AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC TCGGCGTTTC 2341 ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt aagttgataC 2401 TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG 2461 CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG 2521 CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT 2581 GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA 2641 TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga tcttactaac 2701 taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 2761 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 2821 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 2881 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 2941 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 3001 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 3061 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 3121 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 3181 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 3241 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 3301 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 3361 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 3421 TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG 3481 CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC 3541 GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA 3601 CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG 3661 GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt taaacagaac 3721 tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG GcAACAGTCT 3781 TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC AGGGCGGCTT 3841 CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA ACCCGTGGTC 3901 GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA TGAACGGTCT 3961 GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct actaactaac 4021 gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT 4081 CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA 4141 CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC 4201 TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA 4261 GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC 4321 AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa gatcctacta 4381 actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC 4441 CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG 4501 TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA 4561 TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA 4621 CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta agtttaaaca 4681 aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG CGGCGGGCCA 4741 TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT 4801 GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC 4861 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA 4921 TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT 4981 AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT 5041 TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat agtactaact 5101 aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG 5161 CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA 5221 CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC 5281 GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC 5341 CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT 5401 TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta 5461 ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG 5521 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 5581 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 5641 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 5701 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 5761 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 5821 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 5881 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 5941 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 6001 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 6061 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 6121 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 6181 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 6241 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 6301 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 6361 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 6421 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 6481 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 6541 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 6601 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 6661 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 6721 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 6781 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 6841 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 6901 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 6961 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 7021 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 7081 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 7141 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 7201 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 7261 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 7321 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 7381 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 7441 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 7501 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 7561 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 7621 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 7681 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 7741 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 7801 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 7861 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 7921 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 7981 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 8041 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 8101 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 8161 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 8221 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 8281 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 8341 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 8401 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 8461 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 8521 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 8581 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 8641 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 8701 AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt 8761 ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA 8821 ACAACTTGGA AATGAAAT // 1484 LOCUS ADDGENE_1484 7610 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_18 complete plasmid sequence KEYWORDS . SOURCE pPD95_18 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1484 at www.addgene.org FEATURES Location/Qualifiers source 1..7610 /organism="Addgene plasmid pPD95_18" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1231 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 635..4188 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 769..819 /label="SynIVS.B" CDS 1270..4191 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1447..1497 /label="SynIVS.D" misc_feature 1888..1938 /label="SynIVS.E" misc_feature 2195..2245 /label="SynIVS.F" misc_feature 2476..2526 /label="SynIVS.G" misc_feature 2763..2813 /label="SynIVS.H" misc_feature 3129..3179 /label="SynIVS.I" misc_feature 3440..3490 /label="SynIVS.J" misc_feature 3846..3896 /label="SynIVS.K" misc_feature 4212..4262 /label="SynIVS.L" promoter 5390..5418 /label="Amp_promoter" CDS 5460..6320 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5460..6320 /label="Ampicillin" /gene="Ampicillin" rep_origin 6475..7094 /label="pBR322_origin" misc_feature 7310..7336 /label="lacI.C" promoter 7403..7432 /label="lac_promoter" misc_feature 7467..7485 /label="M13r_primer" misc_feature 7499..7549 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGaAGgt aagtttaaac 781 agatccatac taactaactt gttctgacat aattttcagC tTGAATGGCG AATGGCGCTT 841 TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA 901 GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA 961 CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC 1021 GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG 1081 AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG TGCAACGGGC GCTGGGTCGG 1141 TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC AAGATCTGAT TAATTTTCag 1201 GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC 1261 CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG 1321 CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC 1381 GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC GCGCTGTACT GGAGGCTGAA 1441 GTcCAGgtaa gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagATG 1501 TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG TGAAACGCAG 1561 GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG ATGAGCGTGG TGGTTATGCC 1621 GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC TGTGGAGCGC CGAAATCCCG 1681 AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG GCACGCTGAT TGAAGCAGAA 1741 GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG GTCTGCTGCT GCTGAACGGC 1801 AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC ATCCTCTGCA TGGTCAGGTC 1861 ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact attcgttact aactaacttt 1921 aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA ACTTTAACGC CGTGCGCTGT 1981 TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG ACCGCTACGG CCTGTATGTG 2041 GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA TGAATCGTCT GACCGATGAT 2101 CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA TGGTGCAGCG CGATCGTAAT 2161 CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt ttaaacagtt gaatactaac 2221 taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT 2281 CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC 2341 ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC 2401 CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG 2461 CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa ctaacacatt agatcctaat 2521 tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG 2581 TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG 2641 ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG 2701 CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA 2761 GCgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt cagGCTGACG 2821 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2881 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2941 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 3001 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 3061 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCaTGGCAG 3121 CAGTGGAGgt aagtttaaac aagatcctac taactaactc tacattgatg aattttcagA 3181 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC 3241 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 3301 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC 3361 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT 3421 GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta ctaactaacg aagatcttga 3481 taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 3541 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 3601 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 3661 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3721 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3781 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3841 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3901 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3961 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 4021 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 4081 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 4141 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 4201 CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc 4261 agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG 4321 CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT 4381 TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA 4441 TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA 4501 ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT 4561 AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT 4621 TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT 4681 CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT 4741 ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC 4801 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC 4861 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT 4921 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC 4981 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 5041 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 5101 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 5161 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 5221 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 5281 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 5341 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5401 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5461 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5521 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5581 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5641 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5701 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5761 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5821 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5881 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5941 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 6001 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 6061 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 6121 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 6181 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 6241 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 6301 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6361 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6421 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6481 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6541 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6601 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6661 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6721 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6781 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6841 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6901 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6961 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 7021 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 7081 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 7141 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 7201 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 7261 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 7321 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7381 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7441 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 7501 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 7561 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1485 LOCUS ADDGENE_1485 7101 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_21 complete plasmid sequence KEYWORDS . SOURCE pPD95_21 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1485 at www.addgene.org FEATURES Location/Qualifiers source 1..7101 /organism="Addgene plasmid pPD95_21" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..3682 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 635..3679 /label="lacZ" /gene="lacZ" misc_feature 3703..3753 /label="SynIVS.L" promoter 4881..4909 /label="Amp_promoter" CDS 4951..5811 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4951..5811 /label="Ampicillin" /gene="Ampicillin" rep_origin 5966..6585 /label="pBR322_origin" misc_feature 6801..6827 /label="lacI.C" promoter 6894..6923 /label="lac_promoter" misc_feature 6958..6976 /label="M13r_primer" misc_feature 6990..7040 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 1141 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 1201 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 1261 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 1321 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT 1741 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1801 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1861 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1921 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1981 GCTGGGGAAT GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC 2041 TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA 2101 TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA 2161 ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA 2221 ATACGCCCAC GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 2281 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 2341 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2401 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT 2461 GACGGAAGCA AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA 2521 AGTGACCAGC GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC 2581 GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA 2641 ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC 2701 AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG 2761 GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT 2821 CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA 2881 ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT 2941 GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA 3001 AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA 3061 GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC 3121 GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG 3181 GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC 3241 GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG 3301 GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG 3361 CTGGGATCTG CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 3421 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 3481 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 3541 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 3601 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3661 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 3721 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 3781 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 3841 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 3901 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3961 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 4021 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 4081 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 4141 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 4201 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 4261 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 4321 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 4381 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 4441 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 4501 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 4561 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 4621 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 4681 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 4741 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 4801 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 4861 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 4921 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4981 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 5041 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 5101 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 5161 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 5221 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 5281 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 5341 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 5401 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 5461 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 5521 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 5581 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 5641 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 5701 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 5761 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 5821 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 5881 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 5941 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 6001 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 6061 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 6121 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 6181 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 6241 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 6301 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 6361 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 6421 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 6481 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 6541 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 6601 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 6661 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 6721 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 6781 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 6841 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 6901 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6961 AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact 7021 attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG 7081 CTAACAACTT GGAAATGAAA T // 1486 LOCUS ADDGENE_1486 7406 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_25 complete plasmid sequence KEYWORDS . SOURCE pPD95_25 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1486 at www.addgene.org FEATURES Location/Qualifiers source 1..7406 /organism="Addgene plasmid pPD95_25" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1180 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLN LT" gene 635..3984 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 1219..3987 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1786..1836 /label="SynIVS.E" misc_feature 2093..2143 /label="SynIVS.F" misc_feature 2610..2660 /label="SynIVS.H" misc_feature 3236..3286 /label="SynIVS.J" misc_feature 3642..3692 /label="SynIVS.K" misc_feature 4008..4058 /label="SynIVS.L" promoter 5186..5214 /label="Amp_promoter" gene 5256..6116 /label="Ampicillin" /gene="Ampicillin" CDS 5256..6116 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6271..6890 /label="pBR322_origin" misc_feature 7106..7132 /label="lacI.C" promoter 7199..7228 /label="lac_promoter" misc_feature 7263..7281 /label="M13r_primer" misc_feature 7295..7345 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGg taagtttaat taagttgata CTAACTAACA AGATCTGATT 1141 AATTTTCagG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA 1201 GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT GGAAGATCAG 1261 GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA 1321 CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG CGCTGTACTG 1381 GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC AGTTTCTTTA 1441 TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA AATTATCGAT 1501 GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA CCCGAAACTG 1561 TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC CGCCGACGGC 1621 ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT TGAAAATGGT 1681 CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA CGAGCATCAT 1741 CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag tttaaactat 1801 tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA GCAGAACAAC 1861 TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC GCTGTGCGAC 1921 CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT GGTGCCAATG 1981 AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT AACGCGAATG 2041 GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA TGgtaagttt 2101 aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG CCACGGCGCT 2161 AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC GGTGCAGTAT 2221 GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA CGCGCGCGTG 2281 GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG GCTTTCGCTA 2341 CCTGGAGAGA CGCGCCCGCT GATCCTTTGC GAATACGCCC ACGCGATGGG TAACAGTCTT 2401 GGCGGTTTCG CTAAATACTG GCAGGCGTTT CGTCAGTATC CCCGTTTACA GGGCGGCTTC 2461 GTCTGGGACT GGGTGGATCA GTCGCTGATT AAATATGATG AAAACGGCAA CCCGTGGTCG 2521 GCTTACGGCG GTGATTTTGG CGATACGCCG AACGATCGCC AGTTCTGTAT GAACGGTCTG 2581 GTCTTTGCCG ACCGCACGCC GCATCCAGCg taagtttaaa caataaccta ctaactaacg 2641 tagataattt aaattttcag GCTGACGGAA GCAAAACACC AGCAGCAGTT TTTCCAGTTC 2701 CGTTTATCCG GGCAAACCAT CGAAGTGACC AGCGAATACC TGTTCCGTCA TAGCGATAAC 2761 GAGCTCCTGC ACTGGATGGT GGCGCTGGAT GGTAAGCCGC TGGCAAGCGG TGAAGTGCCT 2821 CTGGATGTCG CTCCACAAGG TAAACAGTTG ATTGAACTGC CTGAACTACC GCAGCCGGAG 2881 AGCGCCGGGC AACTCTGGCT CACAGTACGC GTAGTGCAAC CGAACGCGAC CGCATGGTCA 2941 GAAGCCGGGC ACATCAGCGC CTGGCAGCAG TGGCGTCTGG CGGAAAACCT CAGTGTGACG 3001 CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA GCGAAATGGA TTTTTGCATC 3061 GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG GCTTTCTTTC ACAGATGTGG 3121 ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC AGTTCACCCG TGCACCGCTG 3181 GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC CTAACGCCTG GGTCGgtaag 3241 tttaaacaaa gttgtactaa ctaacgaaga tcttgataat tttcagAACG CTGGAAGGCG 3301 GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT 3361 GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC 3421 CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG 3481 GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA 3541 GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC 3601 GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT Agtaagttta aacttgatag 3661 tactaactaa catgtttcat ttaaattttc agTACCCCGT ACGTCTTCCC GAGCGAAAAC 3721 GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC 3781 CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG 3841 CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC 3901 GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT 3961 TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta agtttaaact 4021 gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC GTGCCTCTGA 4081 CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT GCTCCCACCC 4141 CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG CTTCTTTTAA 4201 GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT AATAAAAAGT 4261 CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA TCTACACAAT 4321 GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA CATCATAGAA 4381 AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC GCAATTTTTA 4441 TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA AAAAGTTTTG 4501 GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT CCTGCTTTTG 4561 CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT TCTTGCTAAA 4621 ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG GTTTGAGGCT 4681 CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT GGGGTTTTTG 4741 CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA CTAGTCGGCC 4801 GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 4861 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 4921 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA 4981 GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 5041 ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 5101 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 5161 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 5221 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 5281 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 5341 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 5401 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 5461 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 5521 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 5581 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 5641 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 5701 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 5761 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 5821 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 5881 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 5941 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 6001 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 6061 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 6121 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 6181 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 6241 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 6301 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 6361 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 6421 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 6481 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 6541 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 6601 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 6661 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 6721 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 6781 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 6841 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 6901 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 6961 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 7021 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 7081 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 7141 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 7201 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 7261 CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt ttaaacatga tcttactaac 7321 taactattct catttaaatt ttcagAGCTT AAAAATGGCT GAAATCACTC ACAACGATGG 7381 ATACGCTAAC AACTTGGAAA TGAAAT // 1487 LOCUS ADDGENE_1487 7254 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_27 complete plasmid sequence KEYWORDS . SOURCE pPD95_27 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1487 at www.addgene.org FEATURES Location/Qualifiers source 1..7254 /organism="Addgene plasmid pPD95_27" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..3835 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLC DRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGN ESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKW SIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRL TEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQ GKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAA SHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDN DIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYP DRLTAACFDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" gene 635..3832 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1736..1786 /label="SynIVS.E" misc_feature 2509..2559 /label="SynIVS.H" misc_feature 3490..3540 /label="SynIVS.K" misc_feature 3856..3906 /label="SynIVS.L" promoter 5034..5062 /label="Amp_promoter" CDS 5104..5964 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5104..5964 /label="Ampicillin" /gene="Ampicillin" rep_origin 6119..6738 /label="pBR322_origin" misc_feature 6954..6980 /label="lacI.C" promoter 7047..7076 /label="lac_promoter" misc_feature 7111..7129 /label="M13r_primer" misc_feature 7143..7193 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 1141 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 1201 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 1261 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 1321 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 1741 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 1801 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 1861 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 1921 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 1981 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 2041 TGAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC TGGATCAAAT CTGTCGATCC 2101 TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC ACGGCCACCG ATATTATTTG 2161 CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG GCTGTGCCGA AATGGTCCAT 2221 CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG ATCCTTTGCG AATACGCCCA 2281 CGCGATGGGT AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC GTCAGTATCC 2341 CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA AATATGATGA 2401 AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA ACGATCGCCA 2461 GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt aagtttaaac 2521 aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG CAAAACACCA 2581 GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA GCGAATACCT 2641 GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG GTAAGCCGCT 2701 GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA TTGAACTGCC 2761 TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG TAGTGCAACC 2821 GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCC TGGCAGCAGT GGCGTCTGGC 2881 GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC ATCCCGCATC TGACCACCAG 2941 CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG CAATTTAACC GCCAGTCAGG 3001 CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG CTGACGCCGC TGCGCGATCA 3061 GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT GAAGCGACCC GCATTGACCC 3121 TAACGCCTGG GTCGAACGCT GGAAGGCGGC GGGCCATTAC CAGGCCGAAG CAGCGTTGTT 3181 GCAGTGCACG GCAGATACAC TTGCTGATGC GGTGCTGATT ACGACCGCTC ACGCGTGGCA 3241 GCATCAGGGG AAAACCTTAT TTATCAGCCG GAAAACCTAC CGGATTGATG GTAGTGGTCA 3301 AATGGCGATT ACCGTTGATG TTGAAGTGGC GAGCGATACA CCGCATCCGG CGCGGATTGG 3361 CCTGAACTGC CAGCTGGCGC AGGTAGCAGA GCGGGTAAAC TGGCTCGGAT TAGGGCCGCA 3421 AGAAAACTAT CCCGACCGCC TTACTGCCGC CTGTTTTGAC CGCTGGGATC TGCCATTGTC 3481 AGACATGTAg taagtttaaa cttgatagta ctaactaaca tgtttcattt aaattttcag 3541 TACCCCGTAC GTCTTCCCGA GCGAAAACGG TCTGCGCTGC GGGACGCGCG AATTGAATTA 3601 TGGCCCACAC CAGTGGCGCG GCGACTTCCA GTTCAACATC AGCCGCTACA GTCAACAGCA 3661 ACTGATGGAA ACCAGCCATC GCCATCTGCT GCACGCGGAA GAAGGCACAT GGCTGAATAT 3721 CGACGGTTTC CATATGGGGA TTGGTGGCGA CGACTCCTGG AGCCCGTCAG TATCGGCGGA 3781 ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG 3841 GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat 3901 tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA 3961 CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT 4021 AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA 4081 AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT 4141 AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC 4201 TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT 4261 ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC 4321 AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA 4381 GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA 4441 TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA 4501 GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA 4561 AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC 4621 AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG 4681 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 4741 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 4801 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 4861 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 4921 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 4981 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 5041 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 5101 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 5161 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 5221 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 5281 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 5341 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 5401 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 5461 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 5521 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 5581 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 5641 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 5701 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 5761 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 5821 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 5881 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 5941 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 6001 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 6061 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 6121 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 6181 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 6241 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 6301 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 6361 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 6421 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 6481 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 6541 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 6601 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 6661 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 6721 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 6781 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 6841 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 6901 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 6961 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 7021 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 7081 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 7141 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 7201 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1488 LOCUS ADDGENE_1488 7228 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_57 complete plasmid sequence KEYWORDS . SOURCE pPD95_57 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1488 at www.addgene.org FEATURES Location/Qualifiers source 1..7228 /organism="Addgene plasmid pPD95_57" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" misc_feature 136..162 /label="SV40.NLS" CDS 136..849 /label="Orf0" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 253..3806 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 387..437 /label="SynIVS.B" CDS 888..3809 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1065..1115 /label="SynIVS.D" misc_feature 1506..1556 /label="SynIVS.E" misc_feature 1813..1863 /label="SynIVS.F" misc_feature 2094..2144 /label="SynIVS.G" misc_feature 2381..2431 /label="SynIVS.H" misc_feature 2747..2797 /label="SynIVS.I" misc_feature 3058..3108 /label="SynIVS.J" misc_feature 3464..3514 /label="SynIVS.K" misc_feature 3830..3880 /label="SynIVS.L" promoter 5008..5036 /label="Amp_promoter" gene 5078..5938 /label="Ampicillin" /gene="Ampicillin" CDS 5078..5938 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6093..6712 /label="pBR322_origin" misc_feature 6928..6954 /label="lacI.C" promoter 7021..7050 /label="lac_promoter" misc_feature 7085..7103 /label="M13r_primer" misc_feature 7117..7167 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 181 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 241 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 301 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 361 GATCGCCCTT CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt 421 tctgacataa ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA 481 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC 541 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT 601 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT 661 AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC 721 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt 781 aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT 841 TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT 901 GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG 961 ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT 1021 TTAATGATGA TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga 1081 tcttactaac taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC 1141 TACGGGTAAC AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT 1201 TCGGCGGTGA AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA 1261 ACGTCGAAAA CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG 1321 AACTGCACAC CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG 1381 AGGTGCGGAT TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG 1441 TTAACCGTCA CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC 1501 AGGATgtaag tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC 1561 TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC 1621 TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA 1681 CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA 1741 GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT 1801 CGCTGGGGAA TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt 1861 cagAATCAGG CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC 1921 CTTCCCGCCC GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT 1981 GCCCGATGTA CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA 2041 TCAAAAAATG GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt 2101 taaacagaac tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG 2161 GcAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC 2221 AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA 2281 ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA 2341 TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct 2401 actaactaac gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT 2461 TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC 2521 ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG 2581 GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC 2641 CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA 2701 CCGCATGGTC AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa 2761 gatcctacta actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA 2821 CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA 2881 TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT 2941 GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC 3001 TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta 3061 agtttaaaca aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG 3121 CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG 3181 ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA 3241 GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG 3301 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG 3361 CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG 3421 CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat 3481 agtactaact aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA 3541 ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT 3601 TCCAGTTCAA CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC 3661 TGCTGCACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 3721 GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC 3781 ATTACCAACT TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa 3841 ctgagttcta ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT 3901 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 3961 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 4021 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 4081 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 4141 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 4201 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 4261 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 4321 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 4381 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 4441 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 4501 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 4561 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 4621 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4681 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4741 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4801 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4861 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4921 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4981 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 5041 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 5101 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 5161 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 5221 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 5281 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 5341 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 5401 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 5461 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 5521 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5581 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5641 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5701 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5761 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5821 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5881 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5941 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 6001 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 6061 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 6121 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 6181 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 6241 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 6301 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 6361 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 6421 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 6481 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6541 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6601 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6661 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6721 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6781 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6841 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6901 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6961 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 7021 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 7081 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 7141 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 7201 GGATACGCTA ACAACTTGGA AATGAAAT // 1489 LOCUS ADDGENE_1489 7390 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_62 complete plasmid sequence KEYWORDS . SOURCE pPD95_62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1489 at www.addgene.org FEATURES Location/Qualifiers source 1..7390 /organism="Addgene plasmid pPD95_62" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" CDS 298..1011 /label="Orf0" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 298..324 /label="SV40.NLS" gene 415..3968 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 549..599 /label="SynIVS.B" CDS 1050..3971 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1227..1277 /label="SynIVS.D" misc_feature 1668..1718 /label="SynIVS.E" misc_feature 1975..2025 /label="SynIVS.F" misc_feature 2256..2306 /label="SynIVS.G" misc_feature 2543..2593 /label="SynIVS.H" misc_feature 2909..2959 /label="SynIVS.I" misc_feature 3220..3270 /label="SynIVS.J" misc_feature 3626..3676 /label="SynIVS.K" misc_feature 3992..4042 /label="SynIVS.L" promoter 5170..5198 /label="Amp_promoter" gene 5240..6100 /label="Ampicillin" /gene="Ampicillin" CDS 5240..6100 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6255..6874 /label="pBR322_origin" misc_feature 7090..7116 /label="lacI.C" promoter 7183..7212 /label="lac_promoter" misc_feature 7247..7265 /label="M13r_primer" misc_feature 7279..7329 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA 361 CTGAGCCGCG ATATTGCCCA GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT 421 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT 481 CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG 541 TTGCGaAGgt aagtttaaac agatccatac taactaactt gttctgacat aattttcagC 601 tTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG 661 CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC 721 GGTTACGATG CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT 781 GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG 841 CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG 901 TGCAACGGGC GCTGGGTCGG TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC 961 AAGATCTGAT TAATTTTCag GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT 1021 TACGCGCCGG AGAAAACCGC CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC 1081 TGGAAGATCA GGATATGTGG CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA 1141 AACCGACTAC ACAAATCAGC GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC 1201 GCGCTGTACT GGAGGCTGAA GTcCAGgtaa gtttaaacag gatcttacta actaacatgc 1261 taacactgaa ttttcagATG TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT 1321 TATGGCAGGG TGAAACGCAG GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG 1381 ATGAGCGTGG TGGTTATGCC GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC 1441 TGTGGAGCGC CGAAATCCCG AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG 1501 GCACGCTGAT TGAAGCAGAA GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG 1561 GTCTGCTGCT GCTGAACGGC AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC 1621 ATCCTCTGCA TGGTCAGGTC ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact 1681 attcgttact aactaacttt aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA 1741 ACTTTAACGC CGTGCGCTGT TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG 1801 ACCGCTACGG CCTGTATGTG GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA 1861 TGAATCGTCT GACCGATGAT CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA 1921 TGGTGCAGCG CGATCGTAAT CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt 1981 ttaaacagtt gaatactaac taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG 2041 CTAATCACGA CGCGCTGTAT CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT 2101 ATGAAGGCGG CGGAGCCGAC ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG 2161 TGGATGAAGA CCAGCCCTTC CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC 2221 TACCTGGAGA GACGCGCCCG CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa 2281 ctaacacatt agatcctaat tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT 2341 TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG 2401 ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG 2461 GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG 2521 CCGACCGCAC GCCGCATCCA GCgtaagttt aaacaataac ctactaacta acgtagataa 2581 tttaaatttt cagGCTGACG GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT 2641 CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC 2701 TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG 2761 TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG 2821 GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG 2881 GGCACATCAG CGCaTGGCAG CAGTGGAGgt aagtttaaac aagatcctac taactaactc 2941 tacattgatg aattttcagA CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC 3001 ACGCCATCCC GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC 3061 GTTGGCAATT TAACCGCCAG TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC 3121 AACTGCTGAC GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG 3181 TAAGTGAAGC GACCCGCATT GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta 3241 ctaactaacg aagatcttga taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG 3301 CCGAAGCAGC GTTGTTGCAG TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA 3361 CCGCTCACGC GTGGCAGCAT CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA 3421 TTGATGGTAG TGGTCAAATG GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC 3481 ATCCGGCGCG GATTGGCCTG AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC 3541 TCGGATTAGG GCCGCAAGAA AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT 3601 GGGATCTGCC ATTGTCAGAC ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt 3661 tcatttaaat tttcagTACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA 3721 CGCGCGAATT GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC 3781 GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG 3841 GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC 3901 CGTCAGTATC GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 3961 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 4021 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 4081 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 4141 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 4201 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 4261 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 4321 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 4381 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 4441 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 4501 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 4561 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 4621 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 4681 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 4741 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 4801 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 4861 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 4921 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 4981 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 5041 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 5101 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 5161 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 5221 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 5281 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 5341 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 5401 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 5461 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 5521 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 5581 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 5641 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 5701 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 5761 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 5821 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 5881 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 5941 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 6001 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 6061 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 6121 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 6181 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 6241 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 6301 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 6361 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 6421 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 6481 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 6541 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 6601 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 6661 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 6721 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 6781 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 6841 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 6901 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 6961 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 7021 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 7081 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 7141 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 7201 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 7261 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 7321 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 7381 GAAATGAAAT // 1490 LOCUS ADDGENE_1490 4532 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_67 complete plasmid sequence KEYWORDS . SOURCE pPD95_67 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1490 at www.addgene.org FEATURES Location/Qualifiers source 1..4532 /organism="Addgene plasmid pPD95_67" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..1048 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 179..1039 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 350..400 /label="SynIVS.R" misc_feature 422..441 /label="gfp.S65C" misc_feature 555..605 /label="SynIVS.S" misc_feature 770..820 /label="SynIVS.T" misc_feature 1134..1184 /label="SynIVS.L" promoter 2312..2340 /label="Amp_promoter" CDS 2382..3242 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2382..3242 /label="Ampicillin" /gene="Ampicillin" rep_origin 3397..4016 /label="pBR322_origin" misc_feature 4232..4258 /label="lacI.C" promoter 4325..4354 /label="lac_promoter" misc_feature 4389..4407 /label="M13r_primer" misc_feature 4421..4471 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 181 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 241 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 301 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 361 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 421 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 481 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 541 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 601 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 661 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 721 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 781 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 841 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 901 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 961 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1021 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1081 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1201 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1261 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1321 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1381 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1441 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1501 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1561 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1621 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1681 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1741 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 1801 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 1861 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 1921 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 1981 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2041 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2101 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2161 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2221 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2281 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2341 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2401 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2461 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2521 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2581 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2641 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2701 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 2761 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 2821 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 2881 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 2941 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3001 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3061 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3121 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3181 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3241 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3301 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3361 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3421 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3481 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3541 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3601 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3661 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3721 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 3781 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 3841 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 3901 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 3961 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4021 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4081 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4141 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4201 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4261 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4321 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4381 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 4441 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 4501 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1491 LOCUS ADDGENE_1491 4531 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_69 complete plasmid sequence KEYWORDS . SOURCE pPD95_69 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1491 at www.addgene.org FEATURES Location/Qualifiers source 1..4531 /organism="Addgene plasmid pPD95_69" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" misc_feature 136..162 /label="SV40.NLS" CDS 136..1047 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 178..1038 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 349..399 /label="SynIVS.R" misc_feature 421..440 /label="gfp.S65C" misc_feature 554..604 /label="SynIVS.S" misc_feature 769..819 /label="SynIVS.T" misc_feature 1133..1183 /label="SynIVS.L" promoter 2311..2339 /label="Amp_promoter" gene 2381..3241 /label="Ampicillin" /gene="Ampicillin" CDS 2381..3241 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3396..4015 /label="pBR322_origin" misc_feature 4231..4257 /label="lacI.C" promoter 4324..4353 /label="lac_promoter" misc_feature 4388..4406 /label="M13r_primer" misc_feature 4420..4470 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG 181 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 241 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 301 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac 361 atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC 421 TgTTATGGTG TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC 481 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 541 AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt 601 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT 661 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA 721 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac 781 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC 841 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT 901 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT 961 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA 1021 CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT 1081 ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt 1141 aaactgagtt ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC 1201 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 1261 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 1321 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 1381 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 1441 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 1501 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 1561 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 1621 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 1681 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 1741 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 1801 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 1861 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 1921 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 1981 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 2041 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 2101 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 2161 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 2221 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 2281 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 2341 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 2401 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 2461 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 2521 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 2581 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 2641 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 2701 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 2761 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 2821 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 2881 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 2941 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 3001 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 3061 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 3121 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 3181 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 3241 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 3301 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 3361 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3421 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3481 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3541 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3601 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3661 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3721 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3781 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3841 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3901 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3961 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4021 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 4081 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 4141 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 4201 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 4261 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 4321 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 4381 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta 4441 ctaactaact attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC 4501 GATGGATACG CTAACAACTT GGAAATGAAA T // 1492 LOCUS ADDGENE_1492 4536 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_70 complete plasmid sequence KEYWORDS . SOURCE pPD95_70 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1492 at www.addgene.org FEATURES Location/Qualifiers source 1..4536 /organism="Addgene plasmid pPD95_70" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 141..1052 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 141..167 /label="SV40.NLS" gene 183..1043 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 354..404 /label="SynIVS.R" misc_feature 426..445 /label="gfp.S65C" misc_feature 559..609 /label="SynIVS.S" misc_feature 774..824 /label="SynIVS.T" misc_feature 1138..1188 /label="SynIVS.L" promoter 2316..2344 /label="Amp_promoter" CDS 2386..3246 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2386..3246 /label="Ampicillin" /gene="Ampicillin" rep_origin 3401..4020 /label="pBR322_origin" misc_feature 4236..4262 /label="lacI.C" promoter 4329..4358 /label="lac_promoter" misc_feature 4393..4411 /label="M13r_primer" misc_feature 4425..4475 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 181 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 241 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 301 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 361 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 421 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 481 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 541 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 601 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 661 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 721 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 781 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 841 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 901 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 961 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1021 TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTCCAAC TGAGCGCCGG 1081 TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta 1141 agtttaaact gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC 1201 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 1261 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 1321 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 1381 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 1441 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 1501 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 1561 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 1621 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 1681 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 1741 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 1801 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 1861 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 1921 CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 1981 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 2041 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 2101 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 2161 TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG 2221 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 2281 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 2341 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 2401 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 2461 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 2521 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 2581 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 2641 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 2701 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 2761 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 2821 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 2881 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 2941 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 3001 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 3061 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 3121 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 3181 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 3241 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 3301 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 3361 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 3421 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 3481 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 3541 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 3601 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 3661 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 3721 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 3781 ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 3841 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 3901 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 3961 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 4021 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 4081 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 4141 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 4201 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 4261 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 4321 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 4381 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt ttaaacatga 4441 tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT GAAATCACTC 4501 ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT // 1493 LOCUS ADDGENE_1493 4530 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_73 complete plasmid sequence KEYWORDS . SOURCE pPD95_73 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1493 at www.addgene.org FEATURES Location/Qualifiers source 1..4530 /organism="Addgene plasmid pPD95_73" /mol_type="other DNA" CDS 9..1046 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" gene 177..1037 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 348..398 /label="SynIVS.R" misc_feature 420..439 /label="gfp.S65C" misc_feature 553..603 /label="SynIVS.S" misc_feature 768..818 /label="SynIVS.T" misc_feature 1132..1182 /label="SynIVS.L" promoter 2310..2338 /label="Amp_promoter" CDS 2380..3240 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2380..3240 /label="Ampicillin" /gene="Ampicillin" rep_origin 3395..4014 /label="pBR322_origin" misc_feature 4230..4256 /label="lacI.C" promoter 4323..4352 /label="lac_promoter" misc_feature 4387..4405 /label="M13r_primer" misc_feature 4419..4469 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTA GAAAAAATGA 181 GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG 241 TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC 301 TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca 361 tatatatact aactaaccct gattatttaa attttcagCC AACACTTGTC ACTACTTTCT 421 gTTATGGTGT TCAATGCTTc TCgAGATACC CAGATCATAT GAAACgGCAT GACTTTTTCA 481 AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA 541 ACTACAAGAC ACgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt 601 cagGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA 661 TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC TATAACTCAC 721 ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT CAAAGTTgta agtttaaaca 781 tgattttact aactaactaa tctgatttaa attttcagAA CTTCAAAATT AGACACAACA 841 TTGAAGATGG AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG 901 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC 961 CCAACGAAAA GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC 1021 ATGGCATGGA TGAACTATAC AAATAGCATT CGTAGAATTC CAACTGAGCG CCGGTCGCTA 1081 CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta 1141 aactgagttc tactaactaa cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT 1201 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 1261 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 1321 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 1381 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 1441 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 1501 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 1561 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 1621 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 1681 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 1741 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 1801 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 1861 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC 1921 GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 1981 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 2041 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 2101 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 2161 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 2221 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2281 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 2341 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2401 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 2461 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 2521 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 2581 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 2641 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2701 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 2761 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2821 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 2881 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 2941 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 3001 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 3061 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3121 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3181 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3241 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3301 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 3361 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 3421 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 3481 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 3541 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 3601 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 3661 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 3721 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3781 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3841 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 3901 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 3961 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 4021 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 4081 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4141 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4201 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4261 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4321 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 4381 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 4441 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 4501 ATGGATACGC TAACAACTTG GAAATGAAAT // 1494 LOCUS ADDGENE_1494 4487 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_75 complete plasmid sequence KEYWORDS . SOURCE pPD95_75 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1494 at www.addgene.org FEATURES Location/Qualifiers source 1..4487 /organism="Addgene plasmid pPD95_75" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" gene 134..994 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 134..1003 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 305..355 /label="SynIVS.R" misc_feature 377..396 /label="gfp.S65C" misc_feature 510..560 /label="SynIVS.S" misc_feature 725..775 /label="SynIVS.T" misc_feature 1089..1139 /label="SynIVS.L" promoter 2267..2295 /label="Amp_promoter" gene 2337..3197 /label="Ampicillin" /gene="Ampicillin" CDS 2337..3197 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3352..3971 /label="pBR322_origin" misc_feature 4187..4213 /label="lacI.C" promoter 4280..4309 /label="lac_promoter" misc_feature 4344..4362 /label="M13r_primer" misc_feature 4376..4426 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGGTAGAA AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT 181 TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA 241 TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC 301 ATGGgtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC 361 ACTTGTCACT ACTTTCTgTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA 421 ACgGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT 481 TTTCAAAGAT GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc 541 atacatattt aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG 601 AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA 661 ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA 721 AGTTgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT 781 CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA 841 TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC 901 TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC 961 AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTCCAA 1021 CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG 1081 TCATCAGAgt aagtttaaac tgagttctac taactaacga gtaatattta aattttcagC 1141 ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC 1201 TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT 1261 TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG 1321 AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT 1381 CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA 1441 TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC 1501 AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT 1561 GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG 1621 AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG 1681 GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA 1741 AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA 1801 GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA 1861 ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 1921 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 1981 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 2041 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 2101 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC 2161 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 2221 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 2281 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 2341 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2401 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2461 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2521 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2581 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2641 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2701 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2761 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2821 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2881 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2941 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 3001 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 3061 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 3121 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 3181 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 3241 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 3301 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3361 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3421 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3481 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3541 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3601 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3661 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3721 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC 3781 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3841 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3901 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3961 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 4021 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 4081 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 4141 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 4201 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 4261 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 4321 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag 4381 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC 4441 TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1495 LOCUS ADDGENE_1495 4486 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_77 complete plasmid sequence KEYWORDS . SOURCE pPD95_77 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1495 at www.addgene.org FEATURES Location/Qualifiers source 1..4486 /organism="Addgene plasmid pPD95_77" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" CDS 133..1002 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 133..993 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 304..354 /label="SynIVS.R" misc_feature 376..395 /label="gfp.S65C" misc_feature 509..559 /label="SynIVS.S" misc_feature 724..774 /label="SynIVS.T" misc_feature 1088..1138 /label="SynIVS.L" promoter 2266..2294 /label="Amp_promoter" CDS 2336..3196 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2336..3196 /label="Ampicillin" /gene="Ampicillin" rep_origin 3351..3970 /label="pBR322_origin" misc_feature 4186..4212 /label="lacI.C" promoter 4279..4308 /label="lac_promoter" misc_feature 4343..4361 /label="M13r_primer" misc_feature 4375..4425 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGGTAGAAA AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT 181 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT 241 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA 301 TGGgtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA 361 CTTGTCACTA CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA 421 CgGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT 481 TTCAAAGATG ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca 541 tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA 601 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA 661 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA 721 GTTgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC 781 AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT 841 ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT 901 GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA 961 GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTCCAAC 1021 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 1081 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 1141 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 1201 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 1261 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 1321 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 1381 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 1441 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 1501 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 1561 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 1621 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 1681 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 1741 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 1801 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 1861 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 1921 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 1981 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 2041 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 2101 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 2161 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 2221 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 2281 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 2341 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 2401 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2461 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 2521 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 2581 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 2641 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 2701 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 2761 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 2821 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 2881 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 2941 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 3001 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 3061 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 3121 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 3181 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 3241 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 3301 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3361 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3421 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3481 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 3541 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3601 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3661 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3721 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 3781 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3841 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 3901 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 3961 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 4021 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 4081 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 4141 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 4201 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 4261 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 4321 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt 4381 ttaaacatga tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT 4441 GAAATCACTC ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT // 1496 LOCUS ADDGENE_1496 4491 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_79 complete plasmid sequence KEYWORDS . SOURCE pPD95_79 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1496 at www.addgene.org FEATURES Location/Qualifiers source 1..4491 /organism="Addgene plasmid pPD95_79" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 138..1007 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 138..998 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 309..359 /label="SynIVS.R" misc_feature 381..400 /label="gfp.S65C" misc_feature 514..564 /label="SynIVS.S" misc_feature 729..779 /label="SynIVS.T" misc_feature 1093..1143 /label="SynIVS.L" promoter 2271..2299 /label="Amp_promoter" CDS 2341..3201 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2341..3201 /label="Ampicillin" /gene="Ampicillin" rep_origin 3356..3975 /label="pBR322_origin" misc_feature 4191..4217 /label="lacI.C" promoter 4284..4313 /label="lac_promoter" misc_feature 4348..4366 /label="M13r_primer" misc_feature 4380..4430 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGGT AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC 181 TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG 241 GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG 301 TTCCATGGgt aagtttaaac atatatatac taactaaccc tgattattta aattttcagC 361 CAACACTTGT CACTACTTTC TgTTATGGTG TTCAATGCTT cTCgAGATAC CCAGATCATA 421 TGAAACgGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA 481 TATTTTTCAA AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact 541 aaccatacat atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA 601 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT 661 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA 721 TCAAAGTTgt aagtttaaac atgattttac taactaacta atctgattta aattttcagA 781 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC 841 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC 901 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG 961 TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT 1021 CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC 1081 GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt 1141 cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT 1201 GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT 1261 TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA 1321 ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT 1381 AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA 1441 TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG 1501 TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA 1561 TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT 1621 TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT 1681 CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT 1741 CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG 1801 TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA 1861 CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG 1921 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG 1981 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT 2041 GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA 2101 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA 2161 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT 2221 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 2281 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT 2341 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 2401 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA 2461 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 2521 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC 2581 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG 2641 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 2701 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC 2761 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 2821 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 2881 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT 2941 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 3001 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT 3061 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 3121 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 3181 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT 3241 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG 3301 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC 3361 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 3421 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG 3481 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA 3541 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA 3601 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG 3661 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 3721 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG 3781 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG 3841 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC 3901 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA 3961 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 4021 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 4081 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 4141 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 4201 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG 4261 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 4321 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg 4381 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag AGCTTAAAAA 4441 TGGCTGAAAT CACTCACAAC GATGGATACG CTAACAACTT GGAAATGAAA T // 1497 LOCUS ADDGENE_1497 4485 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_81 complete plasmid sequence KEYWORDS . SOURCE pPD95_81 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1497 at www.addgene.org FEATURES Location/Qualifiers source 1..4485 /organism="Addgene plasmid pPD95_81" /mol_type="other DNA" CDS 9..1001 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPVEKMSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLN IYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY NYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQ NTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" gene 132..992 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 303..353 /label="SynIVS.R" misc_feature 375..394 /label="gfp.S65C" misc_feature 508..558 /label="SynIVS.S" misc_feature 723..773 /label="SynIVS.T" misc_feature 1087..1137 /label="SynIVS.L" promoter 2265..2293 /label="Amp_promoter" CDS 2335..3195 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2335..3195 /label="Ampicillin" /gene="Ampicillin" rep_origin 3350..3969 /label="pBR322_origin" misc_feature 4185..4211 /label="lacI.C" promoter 4278..4307 /label="lac_promoter" misc_feature 4342..4360 /label="M13r_primer" misc_feature 4374..4424 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG 181 AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG 241 CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT 301 GGgtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagCCAACAC 361 TTGTCACTAC TTTCTgTTAT GGTGTTCAAT GCTTcTCgAG ATACCCAGAT CATATGAAAC 421 gGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT 481 TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca gttcggtact aactaaccat 541 acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATAGAA 601 TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAATTGGAAT 661 ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT GGAATCAAAG 721 TTgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagAACTTCA 781 AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT CAACAAAATA 841 CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC ACACAATCTG 901 CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG 961 CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAATA GCATTCGTAG AATTCCAACT 1021 GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG GGCCGCTGTC 1081 ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagCAT 1141 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 1201 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 1261 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 1321 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 1381 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 1441 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 1501 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 1561 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 1621 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 1681 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 1741 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 1801 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 1861 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 1921 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 1981 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 2041 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 2101 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 2161 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 2221 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 2281 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 2341 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 2401 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 2461 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 2521 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 2581 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 2641 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 2701 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 2761 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 2821 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 2881 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 2941 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 3001 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 3061 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 3121 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 3181 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 3241 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 3301 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 3361 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 3421 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 3481 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 3541 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 3601 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 3661 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 3721 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 3781 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 3841 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 3901 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 3961 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 4021 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 4081 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 4141 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 4201 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 4261 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 4321 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt 4381 taaacatgat cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG 4441 AAATCACTCA CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1498 LOCUS ADDGENE_1498 4463 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_85 complete plasmid sequence KEYWORDS . SOURCE pPD95_85 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1498 at www.addgene.org FEATURES Location/Qualifiers source 1..4463 /organism="Addgene plasmid pPD95_85" /mol_type="other DNA" CDS 1..1113 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW LAKMHKVLLALFFIFLAPASALAVSEPVLASTVPVEKMSKGEELFTGVVPILVELDGD VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVT TFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PY IFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI KVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS TQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 79..120 /label="SynIVS.A" misc_feature 142..207 /label="ATG-SecSig" gene 244..1104 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 415..465 /label="SynIVS.R" misc_feature 487..506 /label="gfp.S65T" misc_feature 620..670 /label="SynIVS.S" misc_feature 835..885 /label="SynIVS.T" misc_feature 1199..1249 /label="SynIVS.L" promoter 2377..2405 /label="Amp_promoter" CDS 2447..3307 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2447..3307 /label="Ampicillin" /gene="Ampicillin" rep_origin 3462..4081 /label="pBR322_origin" misc_feature 4297..4323 /label="lacI.C" promoter 4390..4419 /label="lac_promoter" misc_feature 4454..4472 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGCTAGCAAA AATGCATAAG GTTTTGCTGG CACTGTTCTT TATCTTTCTG 181 GCACCAGCAT CCGCACTGGC AGTCTCCGAA CCGGTCCTAG CGTCGACGGT ACCGGTAGAA 241 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 301 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 361 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 421 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 481 ACTTTCaCTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA ACgGCATGAC 541 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 601 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 661 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 721 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 781 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 841 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 901 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 961 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 1021 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 1081 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTCCAA CTGAGCGCCG 1141 GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt 1201 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC 1261 CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG 1321 TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA 1381 GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG 1441 TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA 1501 ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA 1561 ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC 1621 CGCAATTTTT ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG 1681 AAAAAGTTTT GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT 1741 TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT 1801 TTCTTGCTAA AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG 1861 GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA 1921 TGGGGTTTTT GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT 1981 ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 2041 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 2101 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 2161 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC 2221 GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA 2281 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT 2341 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 2401 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 2461 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 2521 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 2581 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 2641 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 2701 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 2761 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 2821 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 2881 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 2941 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 3001 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 3061 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 3121 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 3181 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 3241 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 3301 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 3361 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 3421 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3481 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3541 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3601 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3661 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3721 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3781 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3841 CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG 3901 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3961 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4021 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4081 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT 4141 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG 4201 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 4261 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 4321 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 4381 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 4441 ACAATTTCAC ACAGGAAACA GCT // 1499 LOCUS ADDGENE_1499 5782 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_86 complete plasmid sequence KEYWORDS . SOURCE pPD95_86 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1499 at www.addgene.org FEATURES Location/Qualifiers source 1..5782 /organism="Addgene plasmid pPD95_86" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2499..2516 /label="rf21.28" promoter 3696..3724 /label="Amp_promoter" CDS 3766..4626 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3766..4626 /label="Ampicillin" /gene="Ampicillin" rep_origin 4781..5400 /label="pBR322_origin" misc_feature 5616..5642 /label="lacI.C" promoter 5709..5738 /label="lac_promoter" misc_feature 5773..5791 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 2461 aatgatacta acataacata gaacattttc agGAGGACCC TTGGCTAGCG TCGACGGTAC 2521 CATGGTATTG ATATCTGAGC TCCGCATCGG CCGCTGTCAT CAGATCGCCA TCTCGCGCCC 2581 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 2641 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 2701 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 2761 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 2821 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 2881 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 2941 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 3001 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 3061 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 3121 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 3181 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 3241 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 3301 CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 3361 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 3421 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 3481 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 3541 TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG 3601 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 3661 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 3721 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 3781 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 3841 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 3901 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 3961 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 4021 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 4081 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 4141 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 4201 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 4261 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 4321 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 4381 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 4441 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 4501 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 4561 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 4621 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 4681 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 4741 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 4801 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 4861 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 4921 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 4981 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 5041 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 5101 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 5161 ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 5221 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 5281 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 5341 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 5401 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 5461 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 5521 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 5581 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 5641 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 5701 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 5761 CAATTTCACA CAGGAAACAG CT // 1500 LOCUS ADDGENE_1500 7661 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_93 complete plasmid sequence KEYWORDS . SOURCE pPD95_93 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1500 at www.addgene.org FEATURES Location/Qualifiers source 1..7661 /organism="Addgene plasmid pPD95_93" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..4423 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPE AVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSL TFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLE AEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPK LWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRH EHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANI ETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYR WIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETR PLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYG GDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHS DNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNA TAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSG FLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAA LLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGG DDSWSPSVSAEFQLSAGRYHYQLVWCQK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4420 /label="lacZ" /gene="lacZ" promoter 5575..5603 /label="Amp_promoter" gene 5645..6505 /label="Ampicillin" /gene="Ampicillin" CDS 5645..6505 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6660..7279 /label="pBR322_origin" misc_feature 7495..7521 /label="lacI.C" promoter 7588..7617 /label="lac_promoter" misc_feature 7652..7670 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGCAGC CTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC 1561 GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG 1621 GCAGATGCAC GGTTACGATG CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA 1681 TCCGCCGTTT GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA 1741 TGAAAGCTGG CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTA ACTCGGCGTT 1801 TCATCTGTGG TGCAACGGGC GCTGGGTCGG TTACGGCCAG GACAGTCGTT TGCCGTCTGA 1861 ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC CTCGCGGTGA TGGTGCTGCG 1921 CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG CGGATGAGCG GCATTTTCCG 1981 TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC GATTTCCATG TTGCCACTCG 2041 CTTTAATGAT GATTTCAGCC GCGCTGTACT GGAGGCTGAA GTTCAGATGT GCGGCGAGTT 2101 GCGTGACTAC CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG 2161 CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC 2221 ACTACGTCTG AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG 2281 TGCGGTGGTT GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT 2341 CGGTTTCCGC GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT 2401 GATTCGAGGC GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA 2461 GACGATGGTG CAGGATATCC TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC 2521 GCATTATCCG AACCATCCGC TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT 2581 GGATGAAGCC AATATTGAAA CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC 2641 GCGCTGGCTA CCGGCGATGA GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA 2701 CCCGAGTGTG ATCATCTGGT CGCTGGGGAA TGAATCAGGC CACGGCGCTA ATCACGACGC 2761 GCTGTATCGC TGGATCAAAT CTGTCGATCC TTCCCGCCCG GTGCAGTATG AAGGCGGCGG 2821 AGCCGACACC ACGGCCACCG ATATTATTTG CCCGATGTAC GCGCGCGTGG ATGAAGACCA 2881 GCCCTTCCCG GCTGTGCCGA AATGGTCCAT CAAAAAATGG CTTTCGCTAC CTGGAGAGAC 2941 GCGCCCGCTG ATCCTTTGCG AATACGCCCA CGCGATGGGT AACAGTCTTG GCGGTTTCGC 3001 TAAATACTGG CAGGCGTTTC GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG 3061 GGTGGATCAG TCGCTGATTA AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG 3121 TGATTTTGGC GATACGCCGA ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA 3181 CCGCACGCCG CATCCAGCGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 3241 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 3301 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 3361 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 3421 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 3481 AGCCGGGCAC ATCAGCGCCT GGCAGCAGTG GCGTCTGGCG GAAAACCTCA GTGTGACGCT 3541 CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC GAAATGGATT TTTGCATCGA 3601 GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC TTTCTTTCAC AGATGTGGAT 3661 TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG TTCACCCGTG CACCGCTGGA 3721 TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT AACGCCTGGG TCGAACGCTG 3781 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3841 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3901 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3961 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 4021 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 4081 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTATA CCCCGTACGT 4141 CTTCCCGAGC GAAAACGGTC TGCGCTGCGG GACGCGCGAA TTGAATTATG GCCCACACCA 4201 GTGGCGCGGC GACTTCCAGT TCAACATCAG CCGCTACAGT CAACAGCAAC TGATGGAAAC 4261 CAGCCATCGC CATCTGCTGC ACGCGGAAGA AGGCACATGG CTGAATATCG ACGGTTTCCA 4321 TATGGGGATT GGTGGCGACG ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT TCCAACTGAG 4381 CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC 4441 AGATCGCCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT 4501 ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT 4561 TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC 4621 AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA 4681 TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT 4741 CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT 4801 TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT 4861 CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA 4921 AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG 4981 ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA 5041 AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG 5101 AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC 5161 CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT 5221 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 5281 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 5341 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 5401 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT 5461 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 5521 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 5581 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 5641 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 5701 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 5761 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 5821 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 5881 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 5941 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 6001 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 6061 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 6121 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 6181 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 6241 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 6301 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 6361 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 6421 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 6481 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 6541 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 6601 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 6661 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 6721 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 6781 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 6841 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 6901 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 6961 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 7021 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 7081 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 7141 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 7201 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 7261 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 7321 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 7381 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 7441 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 7501 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 7561 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 7621 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1501 LOCUS ADDGENE_1501 8217 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_02 complete plasmid sequence KEYWORDS . SOURCE pPD96_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1501 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid pPD96_02" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1502 LOCUS ADDGENE_1502 8111 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_04 complete plasmid sequence KEYWORDS . SOURCE pPD96_04 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1502 at www.addgene.org FEATURES Location/Qualifiers source 1..8111 /organism="Addgene plasmid pPD96_04" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..1732 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 179..1039 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 350..400 /label="SynIVS.R" misc_feature 422..441 /label="gfp.S65C" misc_feature 555..605 /label="SynIVS.S" misc_feature 770..820 /label="SynIVS.T" gene 1136..4689 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1270..1320 /label="SynIVS.B" CDS 1771..4692 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1948..1998 /label="SynIVS.D" misc_feature 2389..2439 /label="SynIVS.E" misc_feature 2696..2746 /label="SynIVS.F" misc_feature 2977..3027 /label="SynIVS.G" misc_feature 3264..3314 /label="SynIVS.H" misc_feature 3630..3680 /label="SynIVS.I" misc_feature 3941..3991 /label="SynIVS.J" misc_feature 4347..4397 /label="SynIVS.K" misc_feature 4713..4763 /label="SynIVS.L" promoter 5891..5919 /label="Amp_promoter" CDS 5961..6821 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5961..6821 /label="Ampicillin" /gene="Ampicillin" rep_origin 6976..7595 /label="pBR322_origin" misc_feature 7811..7837 /label="lacI.C" promoter 7904..7933 /label="lac_promoter" misc_feature 7968..7986 /label="M13r_primer" misc_feature 8000..8050 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 181 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 241 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 301 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 361 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 421 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 481 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 541 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 601 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 661 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 721 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 781 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 841 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 901 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 961 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1021 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1081 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1141 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1201 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1261 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1321 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1381 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1441 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1501 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1561 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1621 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1681 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1741 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 1801 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 1861 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 1921 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 1981 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2041 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2101 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2161 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2221 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2281 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2341 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2401 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2461 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2521 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2581 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2641 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2701 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 2761 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 2821 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 2881 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 2941 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3001 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3061 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3121 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3181 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3241 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3301 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3361 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3421 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3481 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3541 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3601 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3661 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3721 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 3781 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 3841 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 3901 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 3961 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4021 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4081 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4141 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4201 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4261 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4321 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4381 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4441 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4501 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4561 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4621 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4681 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4741 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 4801 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 4861 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 4921 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 4981 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5041 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5101 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5161 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5221 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5281 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5341 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5401 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5461 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5521 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5581 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5641 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5701 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 5761 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 5821 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 5881 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 5941 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6001 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6061 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6121 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6181 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6241 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6301 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6361 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6421 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6481 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6541 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6601 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6661 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6721 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 6781 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 6841 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 6901 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6961 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7021 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7081 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7141 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7201 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7261 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7321 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7381 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7441 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7501 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7561 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7621 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7681 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7741 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 7801 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 7861 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 7921 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 7981 ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact attctcattt 8041 aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG CTAACAACTT 8101 GGAAATGAAA T // 1503 LOCUS ADDGENE_1503 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_12 complete plasmid sequence KEYWORDS . SOURCE pPD96_12 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1503 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD96_12" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 653..676 /label="gfp.S65S" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTCTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1504 LOCUS ADDGENE_1504 4574 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_32 complete plasmid sequence KEYWORDS . SOURCE pPD96_32 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1504 at www.addgene.org FEATURES Location/Qualifiers source 1..4574 /organism="Addgene plasmid pPD96_32" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 134..207 /label="ATG-MitLS" CDS 134..1090 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MALLQSRLLLSAPRRAAATARASSVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" gene 221..1081 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 392..442 /label="SynIVS.R" misc_feature 464..483 /label="gfp.S65C" misc_feature 597..647 /label="SynIVS.S" misc_feature 812..862 /label="SynIVS.T" misc_feature 1176..1226 /label="SynIVS.L" promoter 2354..2382 /label="Amp_promoter" CDS 2424..3284 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2424..3284 /label="Ampicillin" /gene="Ampicillin" rep_origin 3439..4058 /label="pBR322_origin" misc_feature 4274..4300 /label="lacI.C" promoter 4367..4396 /label="lac_promoter" misc_feature 4431..4449 /label="M13r_primer" misc_feature 4463..4513 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCTCGAGAA AAAATGGCAC TCCTGCAATC ACGTCTCCTG CTATCCGCAC CTCGTCGTGC 181 CGCTGCAACT GCAAGAGCTT CATCCGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT 241 TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT 301 TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT 361 TTGCACTACT GGAAAACTAC CTGTTCCATG Ggtaagttta aacatatata tactaactaa 421 ccctgattat ttaaattttc agCCAACACT TGTCACTACT TTCTgTTATG GTGTTCAATG 481 CTTcTCgAGA TACCCAGATC ATATGAAACg GCATGACTTT TTCAAGAGTG CCATGCCCGA 541 AGGTTATGTA CAGGAAAGAA CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa 601 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagGTG CTGAAGTCAA 661 GTTTGAAGGT GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA 721 TGGAAACATT CTTGGACACA AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT 781 GGCAGACAAA CAAAAGAATG GAATCAAAGT Tgtaagttta aacatgattt tactaactaa 841 ctaatctgat ttaaattttc agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT 901 TCAACTAGCA GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC 961 AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA 1021 CCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT 1081 ATACAAATAG CATTCGTAGA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 1141 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 1201 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 1261 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 1321 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 1381 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 1441 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 1501 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 1561 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 1621 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 1681 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 1741 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 1801 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 1861 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 1921 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 1981 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 2041 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 2101 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 2161 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 2221 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 2281 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 2341 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 2401 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 2461 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 2521 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 2581 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 2641 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 2701 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 2761 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 2821 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 2881 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 2941 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 3001 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 3061 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 3121 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 3181 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 3241 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 3301 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 3361 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 3421 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 3481 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 3541 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 3601 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 3661 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 3721 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 3781 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 3841 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 3901 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 3961 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 4021 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 4081 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 4141 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 4201 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 4261 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 4321 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 4381 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 4441 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 4501 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 4561 CTTGGAAATG AAAT // 1505 LOCUS ADDGENE_1505 8115 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_62 complete plasmid sequence KEYWORDS . SOURCE pPD96_62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1505 at www.addgene.org FEATURES Location/Qualifiers source 1..8115 /organism="Addgene plasmid pPD96_62" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 141..1736 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 141..167 /label="SV40.NLS" gene 183..1043 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 354..404 /label="SynIVS.R" misc_feature 426..445 /label="gfp.S65C" misc_feature 559..609 /label="SynIVS.S" misc_feature 774..824 /label="SynIVS.T" gene 1140..4693 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1274..1324 /label="SynIVS.B" CDS 1775..4696 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1952..2002 /label="SynIVS.D" misc_feature 2393..2443 /label="SynIVS.E" misc_feature 2700..2750 /label="SynIVS.F" misc_feature 2981..3031 /label="SynIVS.G" misc_feature 3268..3318 /label="SynIVS.H" misc_feature 3634..3684 /label="SynIVS.I" misc_feature 3945..3995 /label="SynIVS.J" misc_feature 4351..4401 /label="SynIVS.K" misc_feature 4717..4767 /label="SynIVS.L" promoter 5895..5923 /label="Amp_promoter" CDS 5965..6825 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5965..6825 /label="Ampicillin" /gene="Ampicillin" rep_origin 6980..7599 /label="pBR322_origin" misc_feature 7815..7841 /label="lacI.C" promoter 7908..7937 /label="lac_promoter" misc_feature 7972..7990 /label="M13r_primer" misc_feature 8004..8054 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 181 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 241 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 301 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 361 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 421 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 481 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 541 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 601 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 661 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 721 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 781 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 841 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 901 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 961 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1021 TTACACATGG CATGGATGAA CTATACAAAT gcCCGGTGGG TGAAGACCAG AAACAGCACC 1081 TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT GTATGGCGAG ATCGATCCCG 1141 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 1201 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC 1261 AACAGTTGCG aAGgtaagtt taaacagatc catactaact aacttgttct gacataattt 1321 tcagCtTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA 1381 GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA 1441 TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC 1501 CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA 1561 GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC 1621 TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGgtaag tttaattaag ttgataCTAA 1681 CTAACAAGAT CTGATTAATT TTCagGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC 1741 ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG 1801 TTATCTGGAA GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT 1861 GCATAAACCG ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT 1921 CtccCGCGCT GTACTGGAGG CTGAAGTcCA Ggtaagttta aacaggatct tactaactaa 1981 catgctaaca ctgaattttc agATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT 2041 TTCTTTATGG CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT 2101 TATCGATGAG CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC 2161 GAAACTGTGG AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC 2221 CGACGGCACG CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA 2281 AAATGGTCTG CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA 2341 GCATCATCCT CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATgtaagttt 2401 aaactattcg ttactaacta actttaaaca tttaaatttt cagATCCTGC TGATGAAGCA 2461 GAACAACTTT AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT 2521 GTGCGACCGC TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT 2581 GCCAATGAAT CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC 2641 GCGAATGGTG CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGg 2701 taagtttaaa cagttgaata ctaactaacg gagatctttg aaattttcag AATCAGGCCA 2761 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 2821 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 2881 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2941 TTCGCTACCT GGAGAGACGC GCCCGCTGAT tCTTTGCGAG gtaagtttaa acagaactct 3001 actaactaac acattagatc ctaattttca gTACGCtCAC GCGATGGGcA ACAGTCTTGG 3061 CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC CGTTTACAGG GCGGCTTCGT 3121 CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA AACGGCAACC CGTGGTCGGC 3181 TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG TTCTGTATGA ACGGTCTGGT 3241 CTTTGCCGAC CGCACGCCGC ATCCAGCgta agtttaaaca ataacctact aactaacgta 3301 gataatttaa attttcagGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 3361 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 3421 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 3481 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 3541 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 3601 AGCCGGGCAC ATCAGCGCaT GGCAGCAGTG GAGgtaagtt taaacaagat cctactaact 3661 aactctacat tgatgaattt tcagACTGGC GGAAAACCTC AGTGTGACGC TCCCCGCCGC 3721 GTCCCACGCC ATCCCGCATC TGACCACCAG CGAAATGGAT TTTTGCATCG AGCTGGGTAA 3781 TAAGCGTTGG CAATTTAACC GCCAGTCAGG CTTTCTTTCA CAGATGTGGA TTGGCGATAA 3841 AAAACAACTG CTGACGCCGC TGCGCGATCA GTTCACCCGT GCACCGCTGG ATAACGACAT 3901 TGGCGTAAGT GAAGCGACCC GCATTGACCC TAACGCCTGG GTCGgtaagt ttaaacaaag 3961 ttgtactaac taacgaagat cttgataatt ttcagAACGC TGGAAGGCGG CGGGCCATTA 4021 CCAGGCCGAA GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT 4081 TACGACCGCT CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA 4141 CCGGATTGAT GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC 4201 ACCGCATCCG GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA 4261 CTGGCTCGGA TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA 4321 CCGCTGGGAT CTGCCATTGT CAGACATGTA gtaagtttaa acttgatagt actaactaac 4381 atgtttcatt taaattttca gTACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG 4441 CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT 4501 CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA 4561 AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG 4621 GAGCCCGTCA GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 4681 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta 4741 actaacgagt aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC 4801 AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT 4861 TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA 4921 CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT 4981 GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC 5041 ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA 5101 CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC 5161 GTCTGGGCCT CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG 5221 GAATTTTTCA ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT 5281 TTCCCCTATT GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT 5341 TGATGAGCAC GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT 5401 GAGTAGAAGT TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC 5461 AGAATACATT CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT 5521 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 5581 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 5641 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 5701 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 5761 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 5821 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 5881 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 5941 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 6001 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 6061 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 6121 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 6181 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 6241 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 6301 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 6361 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 6421 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 6481 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 6541 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 6601 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 6661 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 6721 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 6781 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 6841 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 6901 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 6961 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 7021 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 7081 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 7141 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 7201 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 7261 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 7321 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 7381 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 7441 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 7501 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 7561 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 7621 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 7681 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 7741 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 7801 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 7861 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 7921 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 7981 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 8041 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 8101 ACTTGGAAAT GAAAT // 1506 LOCUS ADDGENE_1506 4970 bp DNA circular SYN 01/03/2005 DEFINITION pPD52_37 complete plasmid sequence KEYWORDS . SOURCE pPD52_37 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1506 at www.addgene.org FEATURES Location/Qualifiers source 1..4970 /organism="Addgene plasmid pPD52_37" /mol_type="other DNA" misc_feature 1639..1680 /label="SynIVS.A" misc_feature 1687..1704 /label="rf21.28" promoter 2884..2912 /label="Amp_promoter" CDS 2954..3814 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2954..3814 /label="Ampicillin" /gene="Ampicillin" rep_origin 3969..4588 /label="pBR322_origin" misc_feature 4804..4830 /label="lacI.C" promoter 4897..4926 /label="lac_promoter" misc_feature 4961..4979 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGgaatt cCCCGGATCC AGATTCGAAC 61 CAGCAATGAC TCTACTGAAG AGCGCGCGCT TTACCACTTC TCCAAACAGG CGAGTGGCGG 121 CCGCGGTTCC AAGGTATATG TGAAACCAAC CGAGCTGTAC GAAGTAGGAA GCTTCCATTA 181 CTTTTTCAGA TATTTAGTTC TGAAAACTTT GTTAACTCTC CTTATCAACA ACTAAATGGC 241 CCTTTTTGTA GTTATTTCTA CTGGAAAAAA ATGTGCCTGT AAATAATATc CTGTTTATAA 301 ACGTTGTTGT cCAGTGAAAA CATTCCCTTG ACCTGAAATT TATCGATAAA AGATAAATTT 361 ATCTTGAAAA CTACtTTTTC GAGTGTATTT TTAAACATTC TACAAATTCC ACTTAATAGC 421 AGGTCGGGAA CTTCGGCGAT TCCTCGAATA AGCTATTTTC AAAAAAAAAA TTAGGCCCAA 481 AATTTGTTTC TGAAAAACGA CCCCAATCTT GTATTTTCCA GTAATAAATA CAATCTGAAT 541 TTTTTTCGAA ATTATTTTTC GCATCGAAAT TGGAAAACGT AGTAAAAAAT ACGGCCGTAG 601 TAAAAGAGTA AACCGTAGTA AAAGAGcaac gtagtaaaag atgaggtagt aaaagagCAT 661 TTGCCTAATT TCTTTTATTT TTCACATTAT ATGTCCAGTT AAAAAAAAAG AACTCTGAGA 721 AATAAATTTT GAAAATCAGG TAAAAAAAGT TAGATGTTTC AGACGTCACC TTGTTGTTaA 781 TATgGTCTCT CATATGATTg GTGACATCAG Aatttttaac ttggaattga ACATTTtaAT 841 TCTTGATTTA TTATTTTCAA GATTTAATTT TAAGTTTTAA AAATAGATTT TTTTTTCAAA 901 AAAAAAACAA GGTTGTAAAA ATTGTTAAAA CTGGAAACAT CTTTTAGCTA TTTTTATTTT 961 AGCCTGCAAG AATTGCTCCT GTGGAGAAAC GTGTAAGTGT ACCGTCGAAA AATGTTGCTG 1021 CGAGGAAGAG AAAAAATCGT GCTGTGAAGG AAAAACCGAG AAGACTGAGC AAAAGAAATA 1081 ACCAATGAGC AAGTGAACTT TTCCCACGTC TACTACTAAA TTATTGTCGA TCTATAATTC 1141 TTTCGCTTAT CAATCTTGTC AATTGAAATA AAACAATATT TTTCTAATTC TTTTTGGAAC 1201 GAACACACGT GTTTAAATGA ATGTTGTGCT AAAAACGTCA CATCAATGGT ACGTGAATGT 1261 TGCAAACACC TTGTCAATAA CTGATAAAAT CAGAAACTAG AGCTGTGACT GAATCGTATA 1321 CTAGAACGGA GTCTCTCTAA AAACGTTCTA AAAACAAACA AAAAATTGTG CAAAGGATTA 1381 GAGTGCTCAA GATCAATGAG CAAACTCACA ATCAACTATC TGTTATTGTT TTGGGTCTCT 1441 TCTATATCTC TATTCTTTTA GTATCATAAG TTTGTACATT GTGACAGGGC CACCCTCTTT 1501 TATCACATAT TTGAAGTGGT AAACAGCCAG CAAAAACCAA ATAAAAAGGC AGTGAGAAAA 1561 AGAAGAAGGC AGCTCAATTT GACTGCTGAA ATTAAGAAct ctCTAGAGGA TCCCCGGGAT 1621 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 1681 GAGGACCCTT GGCTAGCGTC GACGGTACCA TGGTATTGAT ATCTGAGCTC CGCATCGGCC 1741 GCTGTCATCA GATCGCCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC 1801 AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA 1861 AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG 1921 TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC 1981 CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT 2041 TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT 2101 ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC 2161 TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA 2221 TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG 2281 TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG 2341 ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT 2401 GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC 2461 CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG 2521 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 2581 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 2641 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 2701 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA 2761 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC 2821 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 2881 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 2941 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 3001 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA 3061 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 3121 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG 3181 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 3241 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 3301 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT 3361 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 3421 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 3481 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 3541 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 3601 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG 3661 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 3721 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 3781 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 3841 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT 3901 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 3961 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 4021 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 4081 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 4141 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 4201 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 4261 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 4321 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG 4381 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 4441 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4501 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4561 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4621 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC 4681 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 4741 CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 4801 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT 4861 TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG 4921 TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1507 LOCUS ADDGENE_1507 5273 bp DNA circular SYN 01/03/2005 DEFINITION pPD54_01 complete plasmid sequence KEYWORDS . SOURCE pPD54_01 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1507 at www.addgene.org FEATURES Location/Qualifiers source 1..5273 /organism="Addgene plasmid pPD54_01" /mol_type="other DNA" misc_feature 1942..1983 /label="SynIVS.A" misc_feature 1990..2007 /label="rf21.28" promoter 3187..3215 /label="Amp_promoter" gene 3257..4117 /label="Ampicillin" /gene="Ampicillin" CDS 3257..4117 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4272..4891 /label="pBR322_origin" misc_feature 5107..5133 /label="lacI.C" promoter 5200..5229 /label="lac_promoter" misc_feature 5264..5282 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGaATT CGTTATTTTG TGTTACTATC 61 TATACGTGAC TACATATAAA ATATTCATTT GTTCTTCTTT TATAAAAGTA AAAAGGTATT 121 TTTTGAGCTC TCAAATTCTT TAATTGATTA TACAACTTTC AAAAATTAAA GCAGGAATCA 181 CATTATAATT TTTGGGATTC CCCGATATCT AAATGGTTGT TTTGCGGATA TTGACTTTCC 241 TTTCAGATGA CTCGGTGGCA CTGCTTTTAC AAATCGATTT TTCTGGAAAA TTTTGAATTA 301 TTTAGCCTAT AAAATCCACA AAACTTACAT CATCCGGATG GCACCTCCTG AATTTAAGAC 361 CATAGTTGAT AGTAGGTGGA GGAATTGTTG GATAACGTGG GTCTTGATGT ACTCTGAAAA 421 ATTTTAAATG GTTGTGTAAT GAGTGTCAGA GCTCACCGAA TTTTTCTGGA GTGATGTCTT 481 GGACGATATG CAATGTTTCT GGGTTTTTGA TGGTGCTTTA TACTATTAAA GTTTCTTAAT 541 ACAGTAACAT CCTCGTTATT TTGAGTATTT CGAGAAGTTG AAGTATTTCT TTGCTTTCTC 601 ATTTTTCTTC TAACTATTCG CTTTGGCTGT TTCTTCACTT TTCTTTTCTT CTTATGTGTT 661 TCCGTTGTGT AAATTGGACC AGGTAGAATA ATCGGTGATT CAACTTCCCG AATAGTCATC 721 ACTTCGTAAT TCATCATTCT ATCGTCATCT TGTTCACGAG AATGATTGTC AAATAATCTT 781 GGCTGCTCTG TAATAACATC TTCCTGAAGA CGAGCTTCAC TAAAGCGCCT TCTGACAGAA 841 TCAGGGCTTG TTGGCACAGA GTTGAATGGG GTCCAGTCGT CTGAAATTTT AAAATTGAAT 901 CACTTTTACG gGTAAGGCAg GATATCACGA TAGTGTCTAA AAGGAGGAAG AATTTTTTCT 961 TGTTTTCTGC CTGGAATTGT TTCAGGATTA AAATAGCAAG CCTCGAAGTT TgCCCGCTGG 1021 CCCGAAATAG CCTATTCATC AGTCAGCCGC AGAAGGGTTG TTTTTGGGGA CCCCGCTTTA 1081 CCGGTGTAGT ATTATTTGAC ATTGGTAATT TTTTAATTAC GGTTAAACAT AAGTTAAACA 1141 ATAAAATTAC AAACCTGCAC AAAGAGTTCC TGGGCCACAT AATGTTTCAT CAATATCACT 1201 TCCCCAGGCG CTATGTCTAT CTTCTGAAAT CTCAGTTTTC TTTTCTAAAT TAAATAATAT 1261 TTCAAACCTT CTACATGTTC TGGAGCGGTT CTGGAACTTT TTTGTCCCAT AGAGAATACA 1321 AAAAGAGACG AAAATGGTTC AGTGGAACGA AACAAACGTG GGATGTAACC ATGGAAGTGA 1381 GAATAATTGA TGGAATAGCT AATGAGCTGA AGCTGAGTAG ATCAGAGACT ACTTTATTCT 1441 AAAAAGTACA GTAGTTGGAA AATTACATGT TTCATTTCTA ATTTTCAAGG AAAAGCTAGT 1501 AATTACCGTA ATCTTGTTTG TACCTGAAAT TTTATGTACT GCAGGTGACC AAGTATGTTT 1561 GAGGCATGAC TTCACACACC TAACTGATAA AGGCCTCTAT CACAAACTAG AGTTGTGACG 1621 GAAAATTCAA CTTCTCAGaA TATAGCTCAA AATCTATCAA ATTTTATTTT CAAAAATCCA 1681 AATAATTGTG CACGCAATGT ACTTACTGCT TCATAAAGTT CAGAAGAATT GGATAAATTT 1741 GAATGAAGTT TTCAAAGCTT TTATCAGTGA CTGTACATTG TGATAGGCTT GTGCTGTTAT 1801 CAGCTGCCTC AAATAGGTTG TCGCTTGAAA ATTTATATAA AAGGCCTACC AGCAGACATG 1861 AGAATCAAGC TTCAAAGGCT CTACTCAAAA ATaGTCTGCA AGTGTCTAGA GGATCCCCGG 1921 GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT 1981 CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCATGGTATT GATATCTGAG CTCCGCATCG 2041 GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC 2101 TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA 2161 AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA 2221 TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC 2281 TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA 2341 TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC 2401 CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC 2461 CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT 2521 CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA 2581 TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC 2641 ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA 2701 GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA 2761 TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC 2821 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA 2881 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT 2941 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 3001 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT 3061 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 3121 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 3181 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 3241 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 3301 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 3361 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 3421 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 3481 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 3541 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 3601 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 3661 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3721 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 3781 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3841 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 3901 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 3961 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 4021 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 4081 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 4141 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 4201 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 4261 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 4321 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 4381 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 4441 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 4501 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 4561 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4621 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA 4681 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4741 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4801 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4861 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4921 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 4981 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 5041 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 5101 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 5161 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 5221 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1508 LOCUS ADDGENE_1508 2997 bp DNA circular SYN 01/03/2005 DEFINITION pPD61_125 complete plasmid sequence KEYWORDS . SOURCE pPD61_125 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1508 at www.addgene.org FEATURES Location/Qualifiers source 1..2997 /organism="Addgene plasmid pPD61_125" /mol_type="other DNA" promoter complement(152..170) /label="T7_promoter" misc_feature 190..338 /label="lacZ_a" misc_feature complement(181..197) /label="M13f20_primer" rep_origin 356..662 /label="f1_origin" promoter 855..883 /label="Amp_promoter" gene 925..1785 /label="Ampicillin" /gene="Ampicillin" CDS 925..1785 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 1940..2559 /label="pBR322_origin" misc_feature 2775..2801 /label="lacI.C" promoter 2868..2897 /label="lac_promoter" misc_feature 2932..2950 /label="M13r_primer" promoter 2967..2986 /label="T3_promoter" ORIGIN 1 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTGGAT CCACCGGTTC CATGGCTAGC 61 CACGTGACGC GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC 121 GACCTCGAGG GGGGGCCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC 181 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG 241 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG 301 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT 361 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 421 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 481 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 541 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 601 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 661 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 721 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 781 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 841 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 901 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 961 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 1021 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 1081 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 1141 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 1201 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 1261 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 1321 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 1381 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 1441 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 1501 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 1561 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 1621 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 1681 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 1741 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 1801 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 1861 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 1921 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 1981 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 2041 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 2101 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 2161 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 2221 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 2281 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 2341 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 2401 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 2461 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 2521 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 2581 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 2641 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 2701 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 2761 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 2821 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 2881 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 2941 TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTG // 1509 1510 LOCUS ADDGENE_1510 10549 bp DNA circular SYN 01/03/2005 DEFINITION BK48 complete plasmid sequence KEYWORDS . SOURCE BK48 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1510 at www.addgene.org FEATURES Location/Qualifiers source 1..10549 /organism="Addgene plasmid BK48" /mol_type="other DNA" misc_feature 28..52 /label="let858.-562" misc_feature 569..592 /label="let858.Tsa" misc_feature 596..619 /label="let858.N" gene 1177..2037 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1177..2406 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVRSRSPRRSSARSPSRSPRRRRERSSERKQSEEPAPLPEKKKK EPLDILRTRTGGAYIPPAKLRLMQQQISDKQSEQYQRMNWERMKKKIHGLVNRVNAKN LVQIVRELLQENVIRSK" misc_feature 1348..1398 /label="SynIVS.R" misc_feature 1420..1439 /label="gfp.S65C" misc_feature 1553..1603 /label="SynIVS.S" misc_feature 1768..1818 /label="SynIVS.T" CDS 6198..6902 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 6878..6902 /label="let858.C" misc_feature 6980..7009 /label="let858.3'" promoter complement(7734..7752) /label="T7_promoter" misc_feature 7757..7917 /label="lacZ_a" misc_feature complement(7760..7776) /label="M13f20_primer" rep_origin 7935..8241 /label="f1_origin" promoter 8435..8463 /label="Amp_promoter" gene 8505..9365 /label="Ampicillin" /gene="Ampicillin" CDS 8505..9365 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 9520..10139 /label="pBR322_origin" misc_feature 10355..10381 /label="lacI.C" misc_feature 10512..10530 /label="M13r_primer" promoter 10547..10566 /label="T3_promoter" ORIGIN 1 TAACCCTCAC TAAAGGGAAC AAAAGCTGGc tccaaaatcg tcttccgctc tgaaaaacga 61 aagtggacct ttgacatccg aaaaaatggg cgaaaaaatg aaattgagct ttttgggtcg 121 aaaaaaatgt ttttagaatg ctgagaacac gttaaacacg aagatcatat ttattttgag 181 acccggatgc tctgaaaatg tctgacatag atttaaaaaa gcatatatat atttttcatt 241 ttcaacgtga aagttttgtg caactttata gaatctccta ttggcacatt gttttttatt 301 taactgaggc agtttttgaa cacctttttg aaactttgaa tctctttgaa gtatactgtc 361 gaaaagactg acttgagcgt tcgaaatgcc agaagaaaac tatatttgaa tctcgcgcta 421 aattgagaaa tgcaaccgcg ctccactgga caattggaaa aaaaatttat tcggaggcga 481 caacggtatt ttcgaaattg attttctgtg tattttctca ttttttataa attcttcttt 541 gatttatcgt tcgtttgtga gaaatttaat tgtattcaaa cttttttata GTAAGATGTC 601 GTCGAGTCGG TCACAATCAC CTGAAACTCC AAAGGCAGCC AGTGAGGAAC GTGAAGAAGA 661 AGAAAAAGAG TCATCTGAAC AGgtttgatt ttctttctgg tcaaaaagat gaaattattg 721 attttcagCC AGATACTCCC AAAACTAGCA GCGAGAAGTC TGCAAGTCGT TCACAGTCGC 781 CCAGAGAATC GCGGGAAGTG AGCCAAGAGg tatgtttttc aaaaatcaat aactgatcat 841 aatttttatt gtttggtgaa tttaagaaaa taatattcga aaattcctct gaattatcaa 901 gattgcagta ttaatttcga gaaaaattga gatattcata gagctattgt aaattttctt 961 gatttcagAC TGAAACTTCG GAAAATCAAG AGAAAATCAA AGAAAAGGAT GACGGGGATG 1021 ATCAGCCTGG CACACCGAAC AGCTATAGAA GCCGGGAAAC TTCACCAGCT CCAAAAAGGT 1081 CCAAGGAGAC CAGgtttgtc aaaagcttcc tgcgattaat tctcatttca atttttcagA 1141 GAATCAGAGT CTCCTGAAAA ATCACCGGTA GAAAAAATGA GTAAAGGAGA AGAACTTTTC 1201 ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT 1261 GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC 1321 ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca tatatatact aactaaccct 1381 gattatttaa attttcagCC AACACTTGTC ACTACTTTCT gTTATGGTGT TCAATGCTTc 1441 TCgAGATACC CAGATCATAT GAAACgGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT 1501 TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC ACgtaagttt 1561 aaacagttcg gtactaacta accatacata tttaaatttt cagGTGCTGA AGTCAAGTTT 1621 GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA 1681 AACATTCTTG GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA 1741 GACAAACAAA AGAATGGAAT CAAAGTTgta agtttaaaca tgattttact aactaactaa 1801 tctgatttaa attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA 1861 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC 1921 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC 1981 ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC 2041 AAATgcCCGG TTCGTTCAAG ATCTCCCAGA AGGTCTTCAG CACGTTCCCC GTCACGATCT 2101 CCTAGACGGC GCCGAGAAAG AAGCTCAGAA AGAAAGCAAT CCGAAGAGCC AGCACCGCTA 2161 CCAGAGAAAA AGAAGAAAGA GCCGCTGGAT ATTCTACGAA CAAGAACCGG AGGAGCATAT 2221 ATTCCACCCG CCAAACTTCG ACTTATGCAA CAACAGATTA GTGATAAGCA AAGTGAACAG 2281 TATCAGAGAA TGAATTGGGA AAGAATGAAG AAAAAGATTC ACGGATTGGT TAACAGAGTC 2341 AACGCGAAGA ATCTTGTTCA AATTGTCAGA GAACTTCTTC AAGAGAATGT GATTCGTTCA 2401 AAgtgagtga gaaaatcgaa ggaaaaggaa agaattaatt taatttttca gGGGACTTCT 2461 CTGCCGTGAC ATTATTCAAG CTCAGGCTTT CTCACCAGGA TTCTCTAACG TCTATGCAGC 2521 TTTGGCGGCA GTTATCAACT CGAAATTCCC TCATGTCGGT GAACTTCTTC TCCGTCGTCT 2581 GATTGTACAG TTCAAAAGAA GTTTCCGTAG AAATGACAGA GGCGTCACGG TGAACGTGAT 2641 CAAATTCATC GCACATTTGA TTAATCAACA AGTTGCTCAC GAAGTTCTTG CGCTGGAAAT 2701 CATGATTCTG ATGCTTGAAG AACCAACTGA TGATTCAGTT GAAGTCGCCA TTGCGTTCCT 2761 GAAAGAGTGT GGAGCAAAGC TTCTGGAGAT TGCTCCAGCA GCTCTTAACA GTGTCTACGA 2821 CCGTCTTCGT GCAATTCTCA TGGAAACTGA AAGATCGGAA AATGCACTGG ATCGACGTAT 2881 TCAGTATATG ATTGAGACTG CAATGCAGAT TCGAAAGGAC AAATTTGCGg taaggtagaa 2941 tatataaata gtttattaga aaaaaataaa ttagaataat ttaaattcct actagccaat 3001 caggcgacct ttttgcgcat agttctatta ttgaaaaatt tggagaattt ctcatattct 3061 cgctcggaaa tctggaattc gacgagatct tctggcttct gtgcagctgc atcgctttgt 3121 gctccctttc tcgcttgtct tctgtgtaca ccaagaacct tgttgagttc atcaactgaa 3181 tctgtgactg gcttgttgct cactggatgc actagacgac tgattctcga gaaatcagat 3241 tgagttgcga ttagggtgac ctagaaattg ggaataatac gaacttttga aaatattcag 3301 gaggattaaa aaaattattc tcgacaatcc tacaaattta cttattgcac catgttgctc 3361 caacattttt cattaaaagt taatgaaaaa atgtagaaaa tcggaaattg gcaattttca 3421 gaccattttt aagcattttc aaaaaaaaat tgcagctgaa ataaatgtca ttttcagata 3481 aatcgagcga ttttctgttg tctgacacta gtttttagtt ttaaaaaatg ttggaagaac 3541 atggtgcaat aggtaatttc atagaatttc catgtgtttt ttttcaatta accaattatc 3601 caaatcttcc aaactcacat tttgcggagc tgggctatca agaatctgct gcagttttat 3661 aagacgagca tctctgatat cactgaaaat taatttttaa tcaaaacttg aatatcaact 3721 aaacccactt attaactttc tcgatcttct gtcgttcggt acgatgacgg tgaagaagcc 3781 aattgtagta gttgatttgg ttcaagtcct ttcggtgttg tacgtcagtg tcctgcaatg 3841 ctatttagtt ataacttagg cctaagattc aatttaatga agtgattaaa tttgttctct 3901 gaacctctta agatgatctt ttggattaga aacatataag acaggtttac ctatctatta 3961 aaaaacagat caaaatagat acgaccaaat cggataatcc atgcctacct ggcatctagg 4021 aacgtgttct tagaagattt cttacgtaat cgtatgaaga aataacaatt tgatcgttgg 4081 ccagcaaaaa tagggtttta agtgggatag tgtttttatt agctaaccgg aaaattttat 4141 agtttttttt tgcaagaaac cactgaaaac cccctaattg tatacatttt ttggagcagc 4201 ttctggtctt tttgagcaat aaaattcgat aaaacagaat ttaagtgtaa attgttcaca 4261 tttagtttct attttatcaa attttgttgc tcaaaaacat tcgaagctgc tctaaaaaaa 4321 tgcattaaaa aaggggtttt cagtggtttt tcacattaaa aaagctaatt ttaactaaaa 4381 atccatcata tttccaactt tgtcacaaca ataaaatgct ggtcaaaatg tgttcgaaaa 4441 aatgtttttt tttttaattt ttataattta aaaatagttt tctttcgctg ggacacatac 4501 atttttgggc gtaaattttc agttcaaatt tccattttta caaccataat cataaagcta 4561 cgtctgatct ctctcgcact tacctgcgcc tgattcgaaa gaacaaccgt agccaaaaga 4621 acaagaagaa caagcacgta gttgtggtag tggacgttca tcacgcaata ctgaccaatg 4681 gtcgtggggt ctcactttcc gtactattga gagaggggag actgaagatg gcaattgagg 4741 acagtgtctt cgacgcacgc atgcatccat aagcataatc caggagggat ggagagaaaa 4801 atcttgtttc taagcccctc cctttgtaat acatacacat atctaatacc gaagaatggc 4861 taattgaatg gacgtcagct gttgctgtag ttgccaaggc atcatcgatg aaataactga 4921 aagaaagaat taaataatta ttgcagGCGT ATCCGGCGGT CATTGAAGAC TTGGACTTGA 4981 TTGAGGAGGA GGATCAGATC ATCCATACAC TTAATTTGGA GGATGCGGTT GATCCGGAAA 5041 ATGGGCTTAg taagtgactg accacacgcg gggggcatta atttaataaa ttgaattcca 5101 tttcagATGT GTTCAAACTA GATCCAGAAT TCGAAAAGAA CGAGGAGGTT TATGAGGAGA 5161 TCCGTAAGGA AATCATTGGA AACGCCGATA TTTCGGATGA GGATGGTGGC GACGAGTTGG 5221 ATGATGAAGA AGAGGGTAGT GATGTGGAAG AGGCTCCGAA GAAGACTACA GAGATTATTG 5281 ATAATACTGA TCAGAATTGA CTGCTTTCAG AAGgtattca ttttgagttt tgggccggca 5341 aatctgtaag ttgccggttg ccgaaaattt gctgaatttg ccggaaaaaa aaattccgga 5401 atttatttaa aaactttttg taaaaattaa attaaatttg caacttttca gAGAAGTCTA 5461 CCTGACAATG CAATCATCTT TGGACTACCA AGAAGCTGCT CACAAATTGC TGAAAATGAA 5521 GATTCCAGAC AGCATGCAGg tcagcgatgt tgcaaagaaa aattttcgac caaaaaaacc 5581 aaccaatcat aaaatttaaa aaaaaactcc gtttttttct ttttttttat acgagaaaaa 5641 ccaaaaaaat gtatttttgc caaattctaa aatactatcc ccgaaatttt caatattttc 5701 tctttcagAA CGAACTCTGC GCGATGCTTG TCGATTGTTG TGCTCAACAG CGTACCTACG 5761 AGCGATTCTA CGGAATGCTC ATCGAACGTT TCTGCCGACT TCGCCTCGAA TACCAGCAAT 5821 ACTTTGAAAA GCTCTGCCAG GACACGTATT CCACGATTCA CCGAATTGAC ATCACAAAAC 5881 TGCGGAATTT GGCTCGCCTT ATTGCTCATT TGCTCTCGAC GGATGCTATT GACTGGAAGA 5941 TTTTGGCCGA TATGAAAATG ACCGAAGAGG ACACAACTTC TTCTGGCAGA ATCTATATTA 6001 AATATATATT TAATGAACTT GTGGAGGCGA TGGGAATGGT TAAACTTCAT TCGAGAGTTA 6061 CTGATCCgtg agtttcctag agagagttgt tttcgtattc aattttccct attttcagAA 6121 CTTTGGCTCA TTGCTTTGTT GGATTATTCC CACGAACTAA TCCGAACAGC GCACGATTTT 6181 CGATCAACTT CTTCACAATG ATTGGATTGG GTGGTTTGAC GTTGGAACTT CGTGAATGGC 6241 TGGCAAAGGG TCTCAAGAAG AAGAAGGGAA TGCTGGATCA GTTGAAGGCC GAATCAAGCT 6301 CAGATTCATC GTCGTCTTCG GATTCGTCAG ACTCGTCTGA TTCTTCGGAT TCTGACGATT 6361 CATCCGACTC GTCTTCAGAT TCCTCATCTT CTTCAGAATC AGAGCCAGAA CCACCGAAGA 6421 AAAAGAAGAA GAAGAACAGT GAAGAGAGTT CCAAAAAGAA GGAAAAAGAG AATATTGGTC 6481 GACGGGATCG TGGAGACAAG AGAGCTGAAC GTCATCGTGA TCAAAGTGTG GAGAACAAGG 6541 ACAAGGATCG TCGACGTCGC CAGGATTCTG ACGAAAATCG TCGGCCAGAA CGAGGAGATG 6601 ACCGCAAGGA TCGGAGTAAA GATCGTCGTC GTCAAGACTC GGATGATGAG GATCGGAAAG 6661 GTCGTGAACG TCGGGAAGAT TCAGGGGAAA GACGTCGCGG AGATCGGGAT CGACGTGATC 6721 GAAACAAGGA TCAGGAGGAT CACCGTGAAG ATCGCCGTGA CCGAAGCAAG GATCGTGAGG 6781 ATCGACGTGA TCGCCGTCGT CATGACTCTG ATGATGATCG TAAAACTCGT CGGGATAGAA 6841 GTGAAGAGCG AGGAGGACGT CGTCGTGAAG TGGAATCGGA TGATCGACGC CGACGTCGTT 6901 GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT 6961 TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt 7021 tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt 7081 atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca 7141 cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga 7201 aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt 7261 aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg caaatgcgaa 7321 tccgtatgga gaggcacgtt tggcgaaggc aaatgttcgg tatggagatc tgtaaaaatt 7381 tttaagttga aatttggtgt tgctctttta caaaattttc cgattttcgc ttgaaattac 7441 ggtgccaggt ctcgacacgt cttccaattt ttcaaattca aaagagcctt taatgggctg 7501 tagttgctaa tttctcgttt ttgaaaattt ttcttccgtt taatcgaaat ttgatgtatt 7561 ttatttatga ttttcaataa atttcaaaga aactggtgaa aactcggaaa attgtgaact 7621 acagtaatcc aatccttaaa ggcgcacacc ttttaaatgt ccgccccaat acgatatttt 7681 tttaagattc gggcccTCTA GAGCGGCCGC CACCGCGGTG GAGCTCCAAT TCGCCCTATA 7741 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 7801 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCCTT CGCCAGCTGG CGTAATAGCG 7861 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGTAG CCTGAATGGC GAATGGGACG 7921 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 7981 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 8041 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 8101 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 8161 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 8221 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 8281 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 8341 CGAATTTTAA CAAAATATTA ACGTTTACAA TTTCAGGTGG CACTTTTCGG GGAAATGTGC 8401 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 8461 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 8521 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 8581 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 8641 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 8701 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 8761 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 8821 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 8881 GCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 8941 TAACCGCTTT TTTTCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 9001 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 9061 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 9121 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 9181 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 9241 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGCAGTCAGG 9301 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 9361 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 9421 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 9481 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 9541 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 9601 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 9661 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 9721 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 9781 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 9841 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 9901 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 9961 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 10021 CAGGGGGGAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 10081 GTCGATTTTT GTGATGCTCG TCAGGGGGGC CGAGCCTATG GAAAAACGCC AGCAACGCGG 10141 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 10201 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 10261 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 10321 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 10381 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTACCTCACT CATTAGGCAC 10441 CCCAGGCTTT ACACTTTATG CTTCCGGCTC CTATGTTGTG TGGAATTGTG AGCGGATAAC 10501 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTCGGAAT // 1511 LOCUS ADDGENE_1511 4913 bp DNA circular SYN 01/03/2005 DEFINITION L2603 complete plasmid sequence KEYWORDS . SOURCE L2603 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1511 at www.addgene.org FEATURES Location/Qualifiers source 1..4913 /organism="Addgene plasmid L2603" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1429 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 560..1420 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 731..781 /label="SynIVS.R" misc_feature 803..822 /label="gfp.S65C" misc_feature 936..986 /label="SynIVS.S" misc_feature 1151..1201 /label="SynIVS.T" misc_feature 1515..1565 /label="SynIVS.L" promoter 2693..2721 /label="Amp_promoter" CDS 2763..3623 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2763..3623 /label="Ampicillin" /gene="Ampicillin" rep_origin 3778..4397 /label="pBR322_origin" misc_feature 4613..4639 /label="lacI.C" promoter 4706..4735 /label="lac_promoter" misc_feature 4770..4788 /label="M13r_primer" misc_feature 4802..4852 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 601 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 661 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 721 TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt taaattttca 781 gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCgAGAT ACCCAGATCA 841 TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC 901 TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa 961 ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT 1021 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA 1081 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG 1141 AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca 1201 gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA 1261 ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC 1321 ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT 1381 TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC ATTCGTAGAA 1441 TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG 1501 CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt 1561 ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC 1621 ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA 1681 ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA 1741 AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA 1801 ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT 1861 GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA 1921 CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA 1981 AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG 2041 TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT 2101 TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG 2161 ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA 2221 AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA 2281 AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA 2341 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 2401 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 2461 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA 2521 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA 2581 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 2641 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 2701 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 2761 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 2821 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 2881 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 2941 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 3001 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 3061 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 3121 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 3181 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 3241 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 3301 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 3361 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 3421 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 3481 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 3541 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 3601 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 3661 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 3721 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 3781 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 3841 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 3901 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 3961 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 4021 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 4081 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 4141 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA 4201 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 4261 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 4321 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 4381 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 4441 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG 4501 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 4561 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT 4621 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT 4681 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG 4741 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 4801 Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA 4861 AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1512 LOCUS ADDGENE_1512 4693 bp DNA circular SYN 01/03/2005 DEFINITION L2604 complete plasmid sequence KEYWORDS . SOURCE L2604 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1512 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L2604" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" misc_feature 298..324 /label="SV40.NLS" CDS 298..1209 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 340..1200 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 511..561 /label="SynIVS.R" misc_feature 583..602 /label="gfp.S65C" misc_feature 716..766 /label="SynIVS.S" misc_feature 931..981 /label="SynIVS.T" misc_feature 1295..1345 /label="SynIVS.L" promoter 2473..2501 /label="Amp_promoter" CDS 2543..3403 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2543..3403 /label="Ampicillin" /gene="Ampicillin" rep_origin 3558..4177 /label="pBR322_origin" misc_feature 4393..4419 /label="lacI.C" promoter 4486..4515 /label="lac_promoter" misc_feature 4550..4568 /label="M13r_primer" misc_feature 4582..4632 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT 361 TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT 421 TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT 481 TGCACTACTG GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac 541 cctgattatt taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC 601 TTcTCgAGAT ACCCAGATCA TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA 661 GGTTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag 721 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG 781 TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT 841 GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG 901 GCAGACAAAC AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac 961 taatctgatt taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT 1021 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA 1081 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC 1141 CACATGGTCC TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA 1201 TACAAATAGC ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT 1261 GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac 1321 taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA 1381 TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA 1441 TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA 1501 ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT 1561 GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC 1621 TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA 1681 AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT 1741 CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA 1801 ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT 1861 CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG 1921 ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA 1981 GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG 2041 AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT 2101 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 2161 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 2221 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 2281 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 2341 CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 2401 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 2461 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 2521 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 2581 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 2641 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 2701 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 2761 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 2821 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 2881 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 2941 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 3001 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 3061 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 3121 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 3181 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 3241 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 3301 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 3361 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 3421 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 3481 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 3541 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3601 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3661 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3721 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3781 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3841 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3901 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 3961 TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 4021 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 4081 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 4141 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4201 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 4261 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 4321 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 4381 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 4441 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 4501 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 4561 TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat 4621 ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC 4681 TTGGAAATGA AAT // 1513 LOCUS ADDGENE_1513 6141 bp DNA circular SYN 01/03/2005 DEFINITION L2605 complete plasmid sequence KEYWORDS . SOURCE L2605 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1513 at www.addgene.org FEATURES Location/Qualifiers source 1..6141 /organism="Addgene plasmid L2605" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" misc_feature 1746..1772 /label="SV40.NLS" CDS 1746..2657 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 1788..2648 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1959..2009 /label="SynIVS.R" misc_feature 2031..2050 /label="gfp.S65C" misc_feature 2164..2214 /label="SynIVS.S" misc_feature 2379..2429 /label="SynIVS.T" misc_feature 2743..2793 /label="SynIVS.L" promoter 3921..3949 /label="Amp_promoter" CDS 3991..4851 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3991..4851 /label="Ampicillin" /gene="Ampicillin" rep_origin 5006..5625 /label="pBR322_origin" misc_feature 5841..5867 /label="lacI.C" promoter 5934..5963 /label="lac_promoter" misc_feature 5998..6016 /label="M13r_primer" misc_feature 6030..6080 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG AGTAAAGGAG 1801 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 1861 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 1921 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac 1981 taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG 2041 TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA 2101 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 2161 CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG 2221 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 2281 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 2341 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac 2401 taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG 2461 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 2521 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 2581 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 2641 ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 2701 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 2761 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 2821 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 2881 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 2941 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3001 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 3061 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 3121 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 3181 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 3241 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 3301 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 3361 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 3421 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 3481 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 3541 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 3601 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 3661 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 3721 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 3781 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 3841 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 3901 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3961 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4021 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 4081 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 4141 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 4201 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 4261 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 4321 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 4381 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 4441 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 4501 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 4561 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 4621 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 4681 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 4741 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 4801 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 4861 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 4921 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 4981 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 5041 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 5101 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 5161 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 5221 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 5281 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 5341 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 5401 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 5461 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 5521 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 5581 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 5641 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 5701 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 5761 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 5821 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 5881 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 5941 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6001 AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact 6061 attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG 6121 CTAACAACTT GGAAATGAAA T // 1514 LOCUS ADDGENE_1514 5740 bp DNA circular SYN 01/03/2005 DEFINITION L2630 complete plasmid sequence KEYWORDS . SOURCE L2630 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1514 at www.addgene.org FEATURES Location/Qualifiers source 1..5740 /organism="Addgene plasmid L2630" /mol_type="other DNA" misc_feature complement(96..126) /label="unc54.enh" misc_feature 164..188 /label="myo2.-1090" CDS 1139..2335 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG KLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGY VQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKED GNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDG SVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHG MDELYK" misc_feature 1252..1275 /label="myo2.5'" misc_feature 1349..1390 /label="SynIVS.A" misc_feature 1424..1450 /label="SV40.NLS" gene 1466..2326 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1637..1687 /label="SynIVS.R" misc_feature 1709..1728 /label="gfp.S65C" misc_feature 1842..1892 /label="SynIVS.S" misc_feature 2057..2107 /label="SynIVS.T" misc_feature 2349..2399 /label="SynIVS.M" misc_feature 2476..2526 /label="SynIVS.L" promoter 3654..3682 /label="Amp_promoter" gene 3724..4584 /label="Ampicillin" /gene="Ampicillin" CDS 3724..4584 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4739..5358 /label="pBR322_origin" misc_feature 5574..5600 /label="lacI.C" promoter 5667..5696 /label="lac_promoter" misc_feature 5731..5749 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCGTCATCCA 61 CAGTGTAATG TAAGATGGTT CAGAGATTCA GAAGAGAGGC AGAGATAGCC TCATCCCACT 121 CAATTGATGA GAGAATGGGA TCCCCAGCTT GCATGCCTGC AGGTCGAGGC ATTTGAATTG 181 GGGGTGGTGG ACAGTAACTG TCTGTAATAA TAATTACTCC TGACCAGGTT GCAATTCGAG 241 TTTTGATAAG CATAATTATA CCTTGTACAT TGTGGGTTTT GTGCTGTGGA CGTTTTATTG 301 TGACATCCCC ATAAGCTACA AGAACAAAAA TGAAATgTAA AAGTATTGAA AACGTCGTAC 361 ATTTTATATC TGAGTAGTAT CCTTTGCTTT AAATGTCCAT AAAAcTAATT TTATAATCAA 421 TAAAACAACG TTTGTAAATC AACTGAGTTT ACAAGTAGAG ACATTGAGGG ATACTTTCAC 481 TATGCTAAAG TGATAATCGA CCAAATAATA ACTtCACTTT GGTATTTATT CCTGTCTTAT 541 AAaTGTTATG TATGAATTAA ATTCATATGC ATATGGCTCA CTCTGACAAt ttttAATAAT 601 CTTCCAGATC AATATTGACT ACCGATGCGG GTGGTCTTTT GCTTTGAATT CTGCTGAACT 661 TTACACCCCG AACAGCAATG TGTGCTTCAG CcTAAAAAAA AGTAAGTGTG TTAATCAGTg 721 CCCCCGATTC TTCATTTTTT GCCCCTCTCT CCCGTTTCGT CGGCAAAAGA AGAGAAAATA 781 AAGATAAGTC TCAAGATAGG TTGGTAATCG CTAAAGTGGT TGTGTGGATA AGAGTAGCAA 841 AATGGCAGGA AGAGCACTTT GCGCGCACAC ACTGTACTCA TTGTTCTGGA TAAAATTCTC 901 TCGTTGTTTG CCGTCGGATG TCTGCCTCTC TGCATTGAGC CGGCTTCTTC ACTATCTTTA 961 GTTAACCTAA AATGCCGTTT CTTTTCTCGT ATCCCcACTA TcCCGTTGAG GTTCTCTGCT 1021 CTCTTCGCTC CCTACCGCCA GCGAGCAACT ATCCGTGGGG GCGCCTTGCT CGGAAGATGG 1081 GGGGGAAGAA AGAAGATTTT TGCTATTTGC ACTTGAGAAA GAGACTTTTC CTGCGTCGAT 1141 GGTTAGAGAA CAGTGTGCAG ACACTTTTCA GCTACCTAGA atTACAatTG GATATCCCCG 1201 CCTCCCAATC CACCCACCCA GGGAAAAAGA AGGGCTCGCC GAAAATCAAA GTTATCTCCA 1261 GGCTCGCGCA TCCCACCGAG CGGTTGACTT CTCTCCACCA CTTTTCATTT TAACCCTCGG 1321 GGTACGGGAT TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA 1381 ACATTTTCAG GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA 1441 GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT 1501 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA 1561 GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA 1621 ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa 1681 ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC 1741 AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA 1801 AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg 1861 tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC 1921 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG 1981 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA 2041 GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa 2101 ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 2161 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 2221 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 2281 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC 2341 GTAGAATTgt aagtttaaac tttctcatac taactaacgc gctctattta aattttcagA 2401 ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG 2461 GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat 2521 tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA 2581 CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT 2641 AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA 2701 AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT 2761 AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC 2821 TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT 2881 ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC 2941 AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA 3001 GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA 3061 TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA 3121 GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA 3181 AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC 3241 AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG 3301 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 3361 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 3421 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 3481 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 3541 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 3601 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 3661 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 3721 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 3781 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 3841 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 3901 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 3961 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 4021 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 4081 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 4141 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 4201 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 4261 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 4321 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 4381 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 4441 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 4501 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 4561 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 4621 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 4681 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 4741 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 4801 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 4861 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 4921 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 4981 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 5041 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 5101 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 5161 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 5221 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 5281 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 5341 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 5401 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 5461 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 5521 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 5581 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 5641 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 5701 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1515 LOCUS ADDGENE_1515 7525 bp DNA circular SYN 01/03/2005 DEFINITION L2678 complete plasmid sequence KEYWORDS . SOURCE L2678 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1515 at www.addgene.org FEATURES Location/Qualifiers source 1..7525 /organism="Addgene plasmid L2678" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..1280 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 684..4237 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 818..868 /label="SynIVS.B" CDS 1319..4240 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1496..1546 /label="SynIVS.D" misc_feature 1937..1987 /label="SynIVS.E" misc_feature 2244..2294 /label="SynIVS.F" misc_feature 2525..2575 /label="SynIVS.G" misc_feature 2812..2862 /label="SynIVS.H" misc_feature 3178..3228 /label="SynIVS.I" misc_feature 3489..3539 /label="SynIVS.J" misc_feature 3895..3945 /label="SynIVS.K" misc_feature 4261..4311 /label="SynIVS.L" promoter 5439..5467 /label="Amp_promoter" CDS 5509..6369 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5509..6369 /label="Ampicillin" /gene="Ampicillin" rep_origin 6524..7143 /label="pBR322_origin" misc_feature 7359..7385 /label="lacI.C" promoter 7452..7481 /label="lac_promoter" misc_feature 7516..7534 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TGGGTGAAGA CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG 661 CGCTGTATGG CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 721 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 781 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact 841 aactaacttg ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC 901 CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG 961 TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA 1021 CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT 1081 CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG 1141 ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg 1201 taagtttaat taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT 1261 GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT 1321 GGTGCTGCGC TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG 1381 CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT 1441 TGCCACTCGC TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag 1501 tttaaacagg atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT 1561 GCGTGACTAC CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG 1621 CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC 1681 ACTACGTCTG AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG 1741 TGCGGTGGTT GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT 1801 CGGTTTCCGC GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT 1861 GATTCGAGGC GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA 1921 GACGATGGTG CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa 1981 ttttcagATC CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC 2041 GAACCATCCG CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC 2101 CAATATTGAA ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT 2161 ACCGGCGATG AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT 2221 GATCATCTGG TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc 2281 tttgaaattt tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA 2341 ATCTGTCGAT CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC 2401 CGATATTATT TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC 2461 GAAATGGTCC ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG 2521 CGAGgtaagt ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC 2581 tCACGCGATG GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA 2641 TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA 2701 TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG 2761 CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta 2821 aacaataacc tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA 2881 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 2941 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 3001 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 3061 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 3121 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta 3181 agtttaaaca agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA 3241 CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT 3301 GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT 3361 TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC 3421 CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC 3481 CTGGGTCGgt aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA 3541 ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA 3601 TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC 3661 CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT 3721 TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT 3781 GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA 3841 CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt 3901 ttaaacttga tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT 3961 CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG 4021 GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG 4081 CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT 4141 GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC 4201 CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA 4261 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG 4321 CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC 4381 TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT 4441 TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT 4501 CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA 4561 AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG 4621 AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG 4681 ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG 4741 TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT 4801 TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT 4861 TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT 4921 TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC 4981 TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC 5041 CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 5101 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 5161 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 5221 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT 5281 GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA 5341 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT 5401 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG 5461 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 5521 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC 5581 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 5641 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 5701 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC 5761 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 5821 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC 5881 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG 5941 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 6001 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG 6061 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 6121 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG 6181 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 6241 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 6301 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG 6361 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 6421 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT 6481 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 6541 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 6601 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC 6661 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 6721 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 6781 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 6841 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 6901 TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG 6961 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 7021 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 7081 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 7141 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG 7201 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC 7261 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA 7321 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT 7381 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG 7441 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 7501 TAACAATTTC ACACAGGAAA CAGCT // 1516 LOCUS ADDGENE_1516 4828 bp DNA circular SYN 01/03/2005 DEFINITION L2680 complete plasmid sequence KEYWORDS . SOURCE L2680 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1516 at www.addgene.org FEATURES Location/Qualifiers source 1..4828 /organism="Addgene plasmid L2680" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..1478 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 609..1469 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 780..830 /label="SynIVS.R" misc_feature 852..871 /label="gfp.S65C" misc_feature 985..1035 /label="SynIVS.S" misc_feature 1200..1250 /label="SynIVS.T" misc_feature 1564..1614 /label="SynIVS.L" promoter 2742..2770 /label="Amp_promoter" gene 2812..3672 /label="Ampicillin" /gene="Ampicillin" CDS 2812..3672 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3827..4446 /label="pBR322_origin" misc_feature 4662..4688 /label="lacI.C" promoter 4755..4784 /label="lac_promoter" misc_feature 4819..4837 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 661 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 721 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 781 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 841 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 901 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 961 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 1021 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 1081 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 1141 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 1201 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 1261 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1321 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1381 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1441 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG 1501 CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC 1561 AGAgtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC 1621 GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT 1681 CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT 1741 TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA 1801 ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC 1861 CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT 1921 TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT 1981 ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA 2041 TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT 2101 AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG 2161 CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG 2221 GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT 2281 GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT 2341 TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 2401 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 2461 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 2521 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 2581 GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT 2641 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA 2701 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 2761 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 2821 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 2881 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 2941 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 3001 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC 3061 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 3121 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 3181 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 3241 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 3301 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA 3361 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 3421 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 3481 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 3541 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 3601 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 3661 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 3721 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 3781 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 3841 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 3901 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 3961 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 4021 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 4081 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 4141 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 4201 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA 4261 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 4321 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 4381 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 4441 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 4501 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 4561 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 4621 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 4681 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 4741 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC 4801 GGATAACAAT TTCACACAGG AAACAGCT // 1517 LOCUS ADDGENE_1517 8407 bp DNA circular SYN 01/03/2005 DEFINITION L2681 complete plasmid sequence KEYWORDS . SOURCE L2681 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1517 at www.addgene.org FEATURES Location/Qualifiers source 1..8407 /organism="Addgene plasmid L2681" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..2162 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 609..1469 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 780..830 /label="SynIVS.R" misc_feature 852..871 /label="gfp.S65C" misc_feature 985..1035 /label="SynIVS.S" misc_feature 1200..1250 /label="SynIVS.T" gene 1566..5119 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1700..1750 /label="SynIVS.B" CDS 2201..5122 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2378..2428 /label="SynIVS.D" misc_feature 2819..2869 /label="SynIVS.E" misc_feature 3126..3176 /label="SynIVS.F" misc_feature 3407..3457 /label="SynIVS.G" misc_feature 3694..3744 /label="SynIVS.H" misc_feature 4060..4110 /label="SynIVS.I" misc_feature 4371..4421 /label="SynIVS.J" misc_feature 4777..4827 /label="SynIVS.K" misc_feature 5143..5193 /label="SynIVS.L" promoter 6321..6349 /label="Amp_promoter" CDS 6391..7251 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6391..7251 /label="Ampicillin" /gene="Ampicillin" rep_origin 7406..8025 /label="pBR322_origin" misc_feature 8241..8267 /label="lacI.C" promoter 8334..8363 /label="lac_promoter" misc_feature 8398..8416 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 661 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 721 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 781 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 841 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 901 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 961 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 1021 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 1081 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 1141 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 1201 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 1261 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1321 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1381 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1441 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC 1501 AGCACCTCGA ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG 1561 ATCCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC 1621 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC 1681 CTTCCCAACA GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca 1741 taattttcag CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC 1801 CGGAAAGCTG GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT 1861 GGCAGATGCA CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA 1921 ATCCGCCGTT TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG 1981 ATGAAAGCTG GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT 2041 TTCATCTGTG GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga 2101 taCTAACTAA CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT 2161 GAGCGCATTT TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA 2221 CGGCAGTTAT CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC 2281 GTTGCTGCAT AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA 2341 TGATTTCtcc CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact 2401 aactaacatg ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT 2461 AACAGTTTCT TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG 2521 TGAAATTATC GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA 2581 AAACCCGAAA CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA 2641 CACCGCCGAC GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG 2701 GATTGAAAAT GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG 2761 TCACGAGCAT CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt 2821 aagtttaaac tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT 2881 GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA 2941 CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG 3001 CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG 3061 CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG 3121 GAATGgtaag tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC 3181 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3241 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3301 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3361 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag 3421 aactctacta actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG 3481 TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG 3541 CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG 3601 GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG 3661 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact 3721 aacgtagata atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA 3781 GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA 3841 TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT 3901 GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC 3961 GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG 4021 GTCAGAAGCC GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta 4081 ctaactaact ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC 4141 CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT 4201 GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG 4261 CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA 4321 CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa 4381 acaaagttgt actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG 4441 CCATTACCAG GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT 4501 GCTGATTACG ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA 4561 AACCTACCGG ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG 4621 CGATACACCG CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG 4681 GGTAAACTGG CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG 4741 TTTTGACCGC TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta 4801 actaacatgt ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 4861 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 4921 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 4981 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 5041 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 5101 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 5161 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 5221 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 5281 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 5341 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 5401 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 5461 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 5521 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 5581 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 5641 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 5701 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 5761 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 5821 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 5881 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 5941 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 6001 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 6061 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 6121 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 6181 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 6241 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 6301 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 6361 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 6421 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 6481 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 6541 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 6601 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 6661 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 6721 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 6781 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 6841 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 6901 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 6961 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 7021 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 7081 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 7141 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 7201 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 7261 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 7321 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 7381 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 7441 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 7501 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 7561 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 7621 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 7681 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 7741 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 7801 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 7861 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 7921 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 7981 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 8041 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 8101 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 8161 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 8221 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 8281 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 8341 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 8401 AACAGCT // 1518 LOCUS ADDGENE_1518 7532 bp DNA circular SYN 01/03/2005 DEFINITION L2682 complete plasmid sequence KEYWORDS . SOURCE L2682 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1518 at www.addgene.org FEATURES Location/Qualifiers source 1..7532 /organism="Addgene plasmid L2682" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..1287 /label="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVGEDQKQHLE LSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSN WQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVN SAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 691..4244 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 825..875 /label="SynIVS.B" CDS 1326..4247 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1503..1553 /label="SynIVS.D" misc_feature 1944..1994 /label="SynIVS.E" misc_feature 2251..2301 /label="SynIVS.F" misc_feature 2532..2582 /label="SynIVS.G" misc_feature 2819..2869 /label="SynIVS.H" misc_feature 3185..3235 /label="SynIVS.I" misc_feature 3496..3546 /label="SynIVS.J" misc_feature 3902..3952 /label="SynIVS.K" misc_feature 4268..4318 /label="SynIVS.L" promoter 5446..5474 /label="Amp_promoter" CDS 5516..6376 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5516..6376 /label="Ampicillin" /gene="Ampicillin" rep_origin 6531..7150 /label="pBR322_origin" misc_feature 7366..7392 /label="lacI.C" promoter 7459..7488 /label="lac_promoter" misc_feature 7523..7541 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 661 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 721 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 781 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GaAGgtaagt ttaaacagat 841 ccatactaac taacttgttc tgacataatt ttcagCtTGA ATGGCGAATG GCGCTTTGCC 901 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC 961 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC 1021 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT 1081 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT 1141 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 1201 GGCCAGgtaa gtttaattaa gttgataCTA ACTAACAAGA TCTGATTAAT TTTCagGACA 1261 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 1321 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 1381 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1441 TCCATGTTGC CACTCGCTTT AATGATGATT TCtccCGCGC TGTACTGGAG GCTGAAGTcC 1501 AGgtaagttt aaacaggatc ttactaacta acatgctaac actgaatttt cagATGTGCG 1561 GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA ACGCAGGTCG 1621 CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT TATGCCGATC 1681 GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA ATCCCGAATC 1741 TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA GCAGAAGCCT 1801 GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG AACGGCAAGC 1861 CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT CAGGTCATGG 1921 ATGAGCAGAC GATGGTGCAG GATgtaagtt taaactattc gttactaact aactttaaac 1981 atttaaattt tcagATCCTG CTGATGAAGC AGAACAACTT TAACGCCGTG CGCTGTTCGC 2041 ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG 2101 ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC 2161 GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT GCAGCGCGAT CGTAATCACC 2221 CGAGTGTGAT CATCTGGTCG CTGGGGAATG gtaagtttaa acagttgaat actaactaac 2281 ggagatcttt gaaattttca gAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT 2341 GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA 2401 CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG 2461 CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA 2521 TtCTTTGCGA Ggtaagttta aacagaactc tactaactaa cacattagat cctaattttc 2581 agTACGCtCA CGCGATGGGc AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC 2641 GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA 2701 AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA 2761 ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt 2821 aagtttaaac aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG 2881 CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA 2941 GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG 3001 GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA 3061 TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG 3121 TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCa TGGCAGCAGT 3181 GGAGgtaagt ttaaacaaga tcctactaac taactctaca ttgatgaatt ttcagACTGG 3241 CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA 3301 GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG 3361 GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC 3421 AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC 3481 CTAACGCCTG GGTCGgtaag tttaaacaaa gttgtactaa ctaacgaaga tcttgataat 3541 tttcagAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA 3601 CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG 3661 GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA 3721 TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT 3781 GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT 3841 ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT 3901 Agtaagttta aacttgatag tactaactaa catgtttcat ttaaattttc agTACCCCGT 3961 ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC 4021 ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG 4081 AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT 4141 TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC 4201 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 4261 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 4321 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 4381 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 4441 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 4501 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 4561 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4621 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4681 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4741 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4801 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4861 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4921 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4981 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 5041 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 5101 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 5161 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 5221 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 5281 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 5341 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 5401 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 5461 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 5521 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 5581 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5641 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5701 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5761 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5821 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5881 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5941 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 6001 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 6061 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 6121 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 6181 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 6241 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 6301 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 6361 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 6421 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 6481 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 6541 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6601 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6661 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6721 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6781 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6841 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6901 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6961 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 7021 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 7081 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 7141 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 7201 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 7261 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 7321 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 7381 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 7441 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 7501 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1519 LOCUS ADDGENE_1519 4835 bp DNA circular SYN 01/03/2005 DEFINITION L2684 complete plasmid sequence KEYWORDS . SOURCE L2684 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1519 at www.addgene.org FEATURES Location/Qualifiers source 1..4835 /organism="Addgene plasmid L2684" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..1485 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVEKMSKGEEL FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYIL TNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNS HNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPI GDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 616..1476 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 787..837 /label="SynIVS.R" misc_feature 859..878 /label="gfp.S65C" misc_feature 992..1042 /label="SynIVS.S" misc_feature 1207..1257 /label="SynIVS.T" misc_feature 1571..1621 /label="SynIVS.L" promoter 2749..2777 /label="Amp_promoter" CDS 2819..3679 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2819..3679 /label="Ampicillin" /gene="Ampicillin" rep_origin 3834..4453 /label="pBR322_origin" misc_feature 4669..4695 /label="lacI.C" promoter 4762..4791 /label="lac_promoter" misc_feature 4826..4844 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT 661 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 721 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 781 CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA 841 ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC AGATCATATG 901 AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 961 TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa 1021 ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1081 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1141 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 1201 AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC 1261 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 1321 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 1381 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 1441 ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC GTAGAATTCC 1501 AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC 1561 TGTCATCAGA gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca 1621 gCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC 1681 TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT 1741 CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT 1801 AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA 1861 TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA 1921 AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT 1981 TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC 2041 ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA 2101 TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG 2161 AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG 2221 GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG 2281 GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA 2341 TAACTGTTTC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 2401 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 2461 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 2521 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 2581 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 2641 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 2701 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 2761 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 2821 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 2881 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 2941 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 3001 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 3061 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 3121 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 3181 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 3241 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 3301 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 3361 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 3421 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 3481 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 3541 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 3601 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 3661 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 3721 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 3781 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3841 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3901 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3961 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 4021 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 4081 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4141 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4201 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 4261 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4321 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4381 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4441 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 4501 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 4561 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 4621 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 4681 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 4741 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 4801 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1520 LOCUS ADDGENE_1520 8414 bp DNA circular SYN 01/03/2005 DEFINITION L2685 complete plasmid sequence KEYWORDS . SOURCE L2685 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1520 at www.addgene.org FEATURES Location/Qualifiers source 1..8414 /organism="Addgene plasmid L2685" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..2169 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVEKMSKGEEL FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYIL TNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNS HNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPI GDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQK QHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVV VPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIF DGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 616..1476 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 787..837 /label="SynIVS.R" misc_feature 859..878 /label="gfp.S65C" misc_feature 992..1042 /label="SynIVS.S" misc_feature 1207..1257 /label="SynIVS.T" gene 1573..5126 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1707..1757 /label="SynIVS.B" CDS 2208..5129 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2385..2435 /label="SynIVS.D" misc_feature 2826..2876 /label="SynIVS.E" misc_feature 3133..3183 /label="SynIVS.F" misc_feature 3414..3464 /label="SynIVS.G" misc_feature 3701..3751 /label="SynIVS.H" misc_feature 4067..4117 /label="SynIVS.I" misc_feature 4378..4428 /label="SynIVS.J" misc_feature 4784..4834 /label="SynIVS.K" misc_feature 5150..5200 /label="SynIVS.L" promoter 6328..6356 /label="Amp_promoter" CDS 6398..7258 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6398..7258 /label="Ampicillin" /gene="Ampicillin" rep_origin 7413..8032 /label="pBR322_origin" misc_feature 8248..8274 /label="lacI.C" promoter 8341..8370 /label="lac_promoter" misc_feature 8405..8423 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT 661 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 721 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 781 CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA 841 ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC AGATCATATG 901 AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 961 TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa 1021 ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1081 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1141 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 1201 AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC 1261 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 1321 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 1381 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 1441 ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATgcCCGGT GGGTGAAGAC 1501 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 1561 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 1621 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 1681 GATCGCCCTT CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt 1741 tctgacataa ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA 1801 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC 1861 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT 1921 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT 1981 AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC 2041 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt 2101 aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT 2161 TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT 2221 GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG 2281 ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT 2341 TTAATGATGA TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga 2401 tcttactaac taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC 2461 TACGGGTAAC AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT 2521 TCGGCGGTGA AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA 2581 ACGTCGAAAA CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG 2641 AACTGCACAC CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG 2701 AGGTGCGGAT TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG 2761 TTAACCGTCA CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC 2821 AGGATgtaag tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC 2881 TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC 2941 TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA 3001 CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA 3061 GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT 3121 CGCTGGGGAA TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt 3181 cagAATCAGG CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC 3241 CTTCCCGCCC GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT 3301 GCCCGATGTA CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA 3361 TCAAAAAATG GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt 3421 taaacagaac tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG 3481 GcAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC 3541 AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA 3601 ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA 3661 TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct 3721 actaactaac gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT 3781 TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC 3841 ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG 3901 GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC 3961 CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA 4021 CCGCATGGTC AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa 4081 gatcctacta actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA 4141 CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA 4201 TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT 4261 GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC 4321 TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta 4381 agtttaaaca aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG 4441 CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG 4501 ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA 4561 GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG 4621 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG 4681 CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG 4741 CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat 4801 agtactaact aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA 4861 ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT 4921 TCCAGTTCAA CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC 4981 TGCTGCACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 5041 GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC 5101 ATTACCAACT TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa 5161 ctgagttcta ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT 5221 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 5281 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 5341 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 5401 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 5461 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 5521 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 5581 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 5641 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 5701 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 5761 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 5821 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 5881 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 5941 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 6001 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 6061 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 6121 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 6181 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 6241 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 6301 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 6361 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 6421 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 6481 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 6541 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 6601 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 6661 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 6721 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 6781 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 6841 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 6901 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 6961 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 7021 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 7081 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 7141 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 7201 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 7261 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 7321 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 7381 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 7441 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 7501 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 7561 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 7621 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 7681 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 7741 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 7801 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 7861 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 7921 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 7981 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 8041 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 8101 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 8161 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 8221 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 8281 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 8341 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 8401 CACAGGAAAC AGCT // 1521 LOCUS ADDGENE_1521 3963 bp DNA circular SYN 01/03/2005 DEFINITION L2822 complete plasmid sequence KEYWORDS . SOURCE L2822 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1521 at www.addgene.org FEATURES Location/Qualifiers source 1..3963 /organism="Addgene plasmid L2822" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1608 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPGDRWSSTAVAAALELVDPPGCRNSISSLSIPSTSRGGPVPNSP YSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLP LGFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 205..1065 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 376..426 /label="SynIVS.R" misc_feature 448..467 /label="gfp.S65C" misc_feature 581..631 /label="SynIVS.S" misc_feature 796..846 /label="SynIVS.T" promoter complement(1204..1222) /label="T7_promoter" misc_feature 1242..1390 /label="lacZ_a" misc_feature complement(1233..1249) /label="M13f20_primer" rep_origin 1408..1714 /label="f1_origin" promoter 1907..1935 /label="Amp_promoter" gene 1977..2837 /label="Ampicillin" /gene="Ampicillin" CDS 1977..2837 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2992..3611 /label="pBR322_origin" misc_feature 3827..3853 /label="lacI.C" promoter 3920..3949 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC TCTAGAACTA 121 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG 181 GGGGGCCCGG TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC 241 CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG 301 GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA 361 CTACCTGTTC CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat 421 tttcagCCAA CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA 481 GATCATATGA AACgGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA 541 AGAACTATAT TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt 601 actaactaac catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC 661 CTTGTTAATA GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA 721 CACAAATTGG AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG 781 AATGGAATCA AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat 841 tttcagAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT 1021 GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA ATgcccgggg 1081 gatcggtgga gctcCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC 1141 AGGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT 1201 TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC 1261 TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC 1321 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 1381 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 1441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 1501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 1561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 1621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 1681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 1741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 1801 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC 1861 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 1921 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 1981 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2041 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2101 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2161 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2221 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2281 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2341 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2401 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2461 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2521 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2581 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 2641 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2701 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 2761 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 2821 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 2881 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 2941 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3001 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3061 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3121 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3181 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3241 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3301 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3361 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 3421 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3481 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3541 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3601 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 3661 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 3721 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 3781 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 3841 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 3901 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 3961 TGA // 1522 LOCUS ADDGENE_1522 9726 bp DNA circular SYN 01/03/2005 DEFINITION L2865 complete plasmid sequence KEYWORDS . SOURCE L2865 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1522 at www.addgene.org FEATURES Location/Qualifiers source 1..9726 /organism="Addgene plasmid L2865" /mol_type="other DNA" misc_feature 577..600 /label="let858.Tsa" CDS 5381..6085 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 6061..6085 /label="let858.C" misc_feature 6163..6192 /label="let858.3'" promoter complement(6911..6929) /label="T7_promoter" misc_feature 6934..7094 /label="lacZ_a" misc_feature complement(6937..6953) /label="M13f20_primer" rep_origin 7112..7418 /label="f1_origin" promoter 7612..7640 /label="Amp_promoter" gene 7682..8542 /label="Ampicillin" /gene="Ampicillin" CDS 7682..8542 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 8697..9316 /label="pBR322_origin" misc_feature 9532..9558 /label="lacI.C" misc_feature 9689..9707 /label="M13r_primer" promoter 9724..9743 /label="T3_promoter" ORIGIN 1 TAACCCTCAC TAAAGGGAAC AAAAGCTGGG GGGGATCctc caaaatcgtc ttccgctctg 61 aaaaacgaaa gtggaccttt gacatccgaa aaaatgggcg aaaaaatgaa attgagcttt 121 ttgggtcgaa aaaaatgttt ttagaatgct gagaacacgt taaacacgaa gatcatattt 181 attttgagac ccggatgctc tgaaaatgtc tgacatagat ttaaaaaagc atatatatat 241 ttttcatttt caacgtgaaa gttttgtgca actttataga atctcctatt ggcacattgt 301 tttttattta actgaggcag tttttgaaca cctttttgaa actttgaatc tctttgaagt 361 atactgtcga aaagactgac ttgagcgttc gaaatgccag aagaaaacta tatttgaatc 421 tcgcgctaaa ttgagaaatg caaccgcgct ccactggaca attggaaaaa aaatttattc 481 ggaggcgaca acggtatttt cgaaattgat tttctgtgta ttttctcatt ttttataaat 541 tcttctttga tttatcgttc gtttgtgaga aatttaattg tattcaaact tttttatagT 601 AAGATaccgg tggtaccgct agccgtacga acccgggttc tagaagcgct gctaaggggg 661 cccTCGTCGA GTCGGTCACA ATCACCTGAA ACTCCAAAGG CAGCCAGTGA GGAACGTGAA 721 GAAGAAGAAA AAGAGTCATC TGAACAGgtt tgattttctt tctggtcaaa aagatgaaat 781 tattgatttt cagCCAGATA CTCCCAAAAC TAGCAGCGAG AAGTCTGCAA GTCGTTCACA 841 GTCGCCCAGA GAATCGCGGG AAGTGAGCCA AGAGgtatgt ttttcaaaaa tcaataactg 901 atcataattt ttattgtttg gtgaatttaa gaaaataata ttcgaaaatt cctctgaatt 961 atcaagattg cagtattaat ttcgagaaaa attgagatat tcatagagct attgtaaatt 1021 ttcttgattt cagACTGAAA CTTCGGAAAA TCAAGAGAAA ATCAAAGAAA AGGATGACGG 1081 GGATGATCAG CCTGGCACAC CGAACAGCTA TAGAAGCCGG GAAACTTCAC CAGCTCCAAA 1141 AAGGTCCAAG GAGACCAGgt ttgtcaaaag cttcctgcga ttaattctca tttcaatttt 1201 tcagAGAATC AGAGTCTCCT GAAAAATCCC CGGTTCGTTC AAGATCTCCC AGAAGGTCTT 1261 CAGCACGTTC CCCGTCACGA TCTCCTAGAC GGCGCCGAGA AAGAAGCTCA GAAAGAAAGC 1321 AATCCGAAGA GCCAGCACCG CTACCAGAGA AAAAGAAGAA AGAGCCGCTG GATATTCTAC 1381 GAACAAGAAC CGGAGGAGCA TATATTCCAC CCGCCAAACT TCGACTTATG CAACAACAGA 1441 TTAGTGATAA GCAAAGTGAA CAGTATCAGA GAATGAATTG GGAAAGAATG AAGAAAAAGA 1501 TTCACGGATT GGTTAACAGA GTCAACGCGA AGAATCTTGT TCAAATTGTC AGAGAACTTC 1561 TTCAAGAGAA TGTGATTCGT TCAAAgtgag tgagaaaatc gaaggaaaag gaaagaatta 1621 atttaatttt tcagGGGACT TCTCTGCCGT GACATTATTC AAGCTCAGGC TTTCTCACCA 1681 GGATTCTCTA ACGTCTATGC AGCTTTGGCG GCAGTTATCA ACTCGAAATT CCCTCATGTC 1741 GGTGAACTTC TTCTCCGTCG TCTGATTGTA CAGTTCAAAA GAAGTTTCCG TAGAAATGAC 1801 AGAGGCGTCA CGGTGAACGT GATCAAATTC ATCGCACATT TGATTAATCA ACAAGTTGCT 1861 CACGAAGTTC TTGCGCTGGA AATCATGATT CTGATGCTTG AAGAACCAAC TGATGATTCA 1921 GTTGAAGTCG CCATTGCGTT CCTGAAAGAG TGTGGAGCAA AGCTTCTGGA GATTGCTCCA 1981 GCAGCTCTTA ACAGTGTCTA CGACCGTCTT CGTGCAATTC TCATGGAAAC TGAAAGATCG 2041 GAAAATGCAC TGGATCGACG TATTCAGTAT ATGATTGAGA CTGCAATGCA GATTCGAAAG 2101 GACAAATTTG CGgtaaggta gaatatataa atagtttatt agaaaaaaat aaattagaat 2161 aatttaaatt cctactagcc aatcaggcga cctttttgcg catagttcta ttattgaaaa 2221 atttggagaa tttctcatat tctcgctcgg aaatctggaa ttcgacgaga tcttctggct 2281 tctgtgcagc tgcatcgctt tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa 2341 ccttgttgag ttcatcaact gaatctgtga ctggcttgtt gctcactgga tgcactagac 2401 gactgattct cgagaaatca gattgagttg cgattagggt gacctagaaa ttgggaataa 2461 tacgaacttt tgaaaatatt caggaggatt aaaaaaatta ttctcgacaa tcctacaaat 2521 ttacttattg caccatgttg ctccaacatt tttcattaaa agttaatgaa aaaatgtaga 2581 aaatcggaaa ttggcaattt tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct 2641 gaaataaatg tcattttcag ataaatcgag cgattttctg ttgtctgaca ctagttttta 2701 gttttaaaaa atgttggaag aacatggtgc aataggtaat ttcatagaat ttccatgtgt 2761 tttttttcaa ttaaccaatt atccaaatct tccaaactca cattttgcgg agctgggcta 2821 tcaagaatct gctgcagttt tataagacga gcatctctga tatcactgaa aattaatttt 2881 taatcaaaac ttgaatatca actaaaccca cttattaact ttctcgatct tctgtcgttc 2941 ggtacgatga cggtgaagaa gccaattgta gtagttgatt tggttcaagt cctttcggtg 3001 ttgtacgtca gtgtcctgca atgctattta gttataactt aggcctaaga ttcaatttaa 3061 tgaagtgatt aaatttgttc tctgaacctc ttaagatgat cttttggatt agaaacatat 3121 aagacaggtt tacctatcta ttaaaaaaca gatcaaaata gatacgacca aatcggataa 3181 tccatgccta cctggcatct aggaacgtgt tcttagaaga tttcttacgt aatcgtatga 3241 agaaataaca atttgatcgt tggccagcaa aaatagggtt ttaagtggga tagtgttttt 3301 attagctaac cggaaaattt tatagttttt ttttgcaaga aaccactgaa aaccccctaa 3361 ttgtatacat tttttggagc agcttctggt ctttttgagc aataaaattc gataaaacag 3421 aatttaagtg taaattgttc acatttagtt tctattttat caaattttgt tgctcaaaaa 3481 cattcgaagc tgctctaaaa aaatgcatta aaaaaggggt tttcagtggt ttttcacatt 3541 aaaaaagcta attttaacta aaaatccatc atatttccaa ctttgtcaca acaataaaat 3601 gctggtcaaa atgtgttcga aaaaatgttt ttttttttaa tttttataat ttaaaaatag 3661 ttttctttcg ctgggacaca tacatttttg ggcgtaaatt ttcagttcaa atttccattt 3721 ttacaaccat aatcataaag ctacgtctga tctctctcgc acttacctgc gcctgattcg 3781 aaagaacaac cgtagccaaa agaacaagaa gaacaagcac gtagttgtgg tagtggacgt 3841 tcatcacgca atactgacca atggtcgtgg ggtctcactt tccgtactat tgagagaggg 3901 gagactgaag atggcaattg aggacagtgt cttcgacgca cgcatgcatc cataagcata 3961 atccaggagg gatggagaga aaaatcttgt ttctaagccc ctccctttgt aatacataca 4021 catatctaat accgaagaat ggctaattga atggacgtca gctgttgctg tagttgccaa 4081 ggcatcatcg atgaaataac tgaaagaaag aattaaataa ttattgcagG CGTATCCGGC 4141 GGTCATTGAA GACTTGGACT TGATTGAGGA GGAGGATCAG ATCATCCATA CACTTAATTT 4201 GGAGGATGCG GTTGATCCGG AAAATGGGCT TAgtaagtga ctgaccacac gcggggggca 4261 ttaatttaat aaattgaatt ccatttcagA TGTGTTCAAA CTAGATCCAG AATTCGAAAA 4321 GAACGAGGAG GTTTATGAGG AGATCCGTAA GGAAATCATT GGAAACGCCG ATATTTCGGA 4381 TGAGGATGGT GGCGACGAGT TGGATGATGA AGAAGAGGGT AGTGATGTGG AAGAGGCTCC 4441 GAAGAAGACT ACAGAGATTA TTGATAATAC TGATCAGAAT TGACTGCTTT CAGAAGgtat 4501 tcattttgag ttttgggccg gcaaatctgt aagttgccgg ttgccgaaaa tttgctgaat 4561 ttgccggaaa aaaaaattcc ggaatttatt taaaaacttt ttgtaaaaat taaattaaat 4621 ttgcaacttt tcagAGAAGT CTACCTGACA ATGCAATCAT CTTTGGACTA CCAAGAAGCT 4681 GCTCACAAAT TGCTGAAAAT GAAGATTCCA GACAGCATGC AGgtcagcga tgttgcaaag 4741 aaaaattttc gaccaaaaaa accaaccaat cataaaattt aaaaaaaaac tccgtttttt 4801 tctttttttt tatacgagaa aaaccaaaaa aatgtatttt tgccaaattc taaaatacta 4861 tccccgaaat tttcaatatt ttctctttca gAACGAACTC TGCGCGATGC TTGTCGATTG 4921 TTGTGCTCAA CAGCGTACCT ACGAGCGATT CTACGGAATG CTCATCGAAC GTTTCTGCCG 4981 ACTTCGCCTC GAATACCAGC AATACTTTGA AAAGCTCTGC CAGGACACGT ATTCCACGAT 5041 TCACCGAATT GACATCACAA AACTGCGGAA TTTGGCTCGC CTTATTGCTC ATTTGCTCTC 5101 GACGGATGCT ATTGACTGGA AGATTTTGGC CGATATGAAA ATGACCGAAG AGGACACAAC 5161 TTCTTCTGGC AGAATCTATA TTAAATATAT ATTTAATGAA CTTGTGGAGG CGATGGGAAT 5221 GGTTAAACTT CATTCGAGAG TTACTGATCC gtgagtttcc tagagagagt tgttttcgta 5281 ttcaattttc cctattttca gAACTTTGGC TCATTGCTTT GTTGGATTAT TCCCACGAAC 5341 TAATCCGAAC AGCGCACGAT TTTCGATCAA CTTCTTCACA ATGATTGGAT TGGGTGGTTT 5401 GACGTTGGAA CTTCGTGAAT GGCTGGCAAA GGGTCTCAAG AAGAAGAAGG GAATGCTGGA 5461 TCAGTTGAAG GCCGAATCAA GCTCAGATTC ATCGTCGTCT TCGGATTCGT CAGACTCGTC 5521 TGATTCTTCG GATTCTGACG ATTCATCCGA CTCGTCTTCA GATTCCTCAT CTTCTTCAGA 5581 ATCAGAGCCA GAACCACCGA AGAAAAAGAA GAAGAAGAAC AGTGAAGAGA GTTCCAAAAA 5641 GAAGGAAAAA GAGAATATTG GTCGACGGGA TCGTGGAGAC AAGAGAGCTG AACGTCATCG 5701 TGATCAAAGT GTGGAGAACA AGGACAAGGA TCGTCGACGT CGCCAGGATT CTGACGAAAA 5761 TCGTCGGCCA GAACGAGGAG ATGACCGCAA GGATCGGAGT AAAGATCGTC GTCGTCAAGA 5821 CTCGGATGAT GAGGATCGGA AAGGTCGTGA ACGTCGGGAA GATTCAGGGG AAAGACGTCG 5881 CGGAGATCGG GATCGACGTG ATCGAAACAA GGATCAGGAG GATCACCGTG AAGATCGCCG 5941 TGACCGAAGC AAGGATCGTG AGGATCGACG TGATCGCCGT CGTCATGACT CTGATGATGA 6001 TCGTAAAACT CGTCGGGATA GAAGTGAAGA GCGAGGAGGA CGTCGTCGTG AAGTGGAATC 6061 GGATGATCGA CGCCGACGTC GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT 6121 TTCCTATTAT TTATTTATTC TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA 6181 ATCCAAATCT AAacattttt ttttctcttt ccgtctccca attcgtattc cgctcctctc 6241 atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg 6301 ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa 6361 tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat 6421 atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat 6481 tgtcctcgct tggcaaatgc gaatccgtat ggagaggcac gtttggcgaa ggcaaatgtt 6541 cggtatggag atctgtaaaa atttttaagt tgaaatttgg tgttgctctt ttacaaaatt 6601 ttccgatttt cgcttgaaat tacggtgcca ggtctcgaca cgtcttccaa tttttcaaat 6661 tcaaaagagc ctttaatggg ctgtagttgc taatttctcg tttttgaaaa tttttcttcc 6721 gtttaatcga aatttgatgt attttattta tgattttcaa taaatttcaa agaaactggt 6781 gaaaactcgg aaaattgtga actacagtaa tccaatcctt aaaggcgcac accttttaaa 6841 tgtccgcccc aatacgatat ttttttaaga ttcgCTAGAG CGGCCGCCAC CGCGGTGGAG 6901 CTCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT TACAACGTCG 6961 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCCTTCGC 7021 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGTAGCCT 7081 GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 7141 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 7201 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 7261 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 7321 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 7381 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 7441 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 7501 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT CAGGTGGCAC 7561 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT 7621 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG 7681 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC 7741 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 7801 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC 7861 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC 7921 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT 7981 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT 8041 ATGCAGTGCT GCCATAAGCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT 8101 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT TCACAACATG GGGGATCATG TAACTCGCCT 8161 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT 8221 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC 8281 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 8341 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 8401 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA 8461 CACGACGGGC AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC 8521 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA 8581 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 8641 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 8701 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 8761 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 8821 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT 8881 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 8941 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 9001 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 9061 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC 9121 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 9181 GCGCACGAGG GAGCTTCCAG GGGGGAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 9241 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCCGA GCCTATGGAA 9301 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 9361 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 9421 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA 9481 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 9541 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 9601 CCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCCTA TGTTGTGTGG 9661 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT 9721 CGGAAT // 1523 LOCUS ADDGENE_1523 4091 bp DNA circular SYN 01/03/2005 DEFINITION L2908 complete plasmid sequence KEYWORDS . SOURCE L2908 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1523 at www.addgene.org FEATURES Location/Qualifiers source 1..4091 /organism="Addgene plasmid L2908" /mol_type="other DNA" gene 195..1055 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 195..1079 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKVSFNI" misc_feature 366..416 /label="SynIVS.R" misc_feature 438..457 /label="gfp.S65C" misc_feature 571..621 /label="SynIVS.S" misc_feature 786..836 /label="SynIVS.T" promoter complement(1246..1264) /label="T7_promoter" misc_feature 1284..1432 /label="lacZ_a" misc_feature complement(1275..1291) /label="M13f20_primer" rep_origin 1450..1756 /label="f1_origin" promoter 1949..1977 /label="Amp_promoter" CDS 2019..2879 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2019..2879 /label="Ampicillin" /gene="Ampicillin" rep_origin 3034..3653 /label="pBR322_origin" misc_feature 3869..3895 /label="lacI.C" promoter 3962..3991 /label="lac_promoter" misc_feature 4026..4044 /label="M13r_primer" promoter 4061..4080 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 241 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 301 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 361 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 421 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA GATCATATGA 481 AACgGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 541 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 601 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 661 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 721 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 781 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 841 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 901 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 961 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1021 CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA Agtaagtttt aacatttaat 1081 catacgaaat gaaagagctc caccgcggtg gcggccgctc tagaactagt ggatccaccg 1141 gttccatggc tagccacgtg acgcgtggat cccccgggct gcaggaattc gatatcaagc 1201 ttatcgatac cgtcgacctc gagggggggc ccggtaccCA ATTCGCCCTA TAGTGAGTCG 1261 TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 1321 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 1381 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC 1441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 1501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 1561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 1621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 1681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 1741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 1801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 1861 TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 1921 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 1981 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 2041 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 2101 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 2161 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 2221 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 2281 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 2341 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 2401 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 2461 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 2521 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 2581 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 2641 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 2701 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 2761 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 2821 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 2881 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 2941 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 3001 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 3061 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 3121 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 3181 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 3241 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 3301 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 3361 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 3421 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 3481 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 3541 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 3601 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 3661 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 3721 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 3781 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 3841 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 3901 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 3961 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 4021 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG 4081 AACAAAAGCT G // 1524 LOCUS ADDGENE_1524 3957 bp DNA circular SYN 01/03/2005 DEFINITION L2911 complete plasmid sequence KEYWORDS . SOURCE L2911 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1524 at www.addgene.org FEATURES Location/Qualifiers source 1..3957 /organism="Addgene plasmid L2911" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" promoter 56..75 /label="T3_promoter" CDS 201..1205 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPGDRWSSTGGGRSRTSGSPGLQEFDIKLIDTVDLEGGPGTQFAL" gene 201..1061 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 372..422 /label="SynIVS.R" misc_feature 444..463 /label="gfp.S65C" misc_feature 577..627 /label="SynIVS.S" misc_feature 792..842 /label="SynIVS.T" promoter complement(1198..1216) /label="T7_promoter" misc_feature 1236..1384 /label="lacZ_a" misc_feature complement(1227..1243) /label="M13f20_primer" rep_origin 1402..1708 /label="f1_origin" promoter 1901..1929 /label="Amp_promoter" gene 1971..2831 /label="Ampicillin" /gene="Ampicillin" CDS 1971..2831 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2986..3605 /label="pBR322_origin" misc_feature 3821..3847 /label="lacI.C" promoter 3914..3943 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC TCTAGAACTA 121 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG 181 GGGCGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 241 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 301 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 361 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 421 agCCAACACT TGTCACTACT TTCTgTTATG GTGTTCAATG CTTcTCgAGA TACCCAGATC 481 ATATGAAACg GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 541 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 601 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 661 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 721 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG 781 GAATCAAAGT Tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 841 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 901 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA 961 CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 1021 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATgc ccgggggatc 1081 ggtggAGCTC CACCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT 1141 TCGATATCAA GCTTATCGAT ACCGTCGACC TCGAGGGGGG GCCCGGTACC CAATTCGCCC 1201 TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 1261 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 1321 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 1381 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 1441 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 1501 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 1561 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 1621 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 1681 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 1741 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 1801 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA 1861 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 1921 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 1981 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 2041 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 2101 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 2161 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG 2221 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 2281 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG 2341 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 2401 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 2461 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA 2521 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 2581 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC 2641 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 2701 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 2761 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA 2821 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC 2881 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 2941 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 3001 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 3061 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 3121 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 3181 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 3241 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 3301 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 3361 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 3421 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 3481 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 3541 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 3601 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG 3661 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC 3721 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA 3781 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT 3841 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 3901 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGA // 1525 LOCUS ADDGENE_1525 4693 bp DNA circular SYN 01/03/2005 DEFINITION L2944 complete plasmid sequence KEYWORDS . SOURCE L2944 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1525 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L2944" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 265..284 /label="gfp.F64L" misc_feature 286..305 /label="gfp.S65T" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTCTCACTTA TGGTGTTCAA 301 TGCTTCTCGA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1526 LOCUS ADDGENE_1526 4693 bp DNA circular SYN 01/03/2005 DEFINITION L2946 complete plasmid sequence KEYWORDS . SOURCE L2946 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1526 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L2946" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFAYGLQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGCTTA TGGTCTTCAA 301 TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1527 LOCUS ADDGENE_1527 4693 bp DNA circular SYN 01/03/2005 DEFINITION L2947 complete plasmid sequence KEYWORDS . SOURCE L2947 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1527 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L2947" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGVQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGGTTA TGGTGTTCAA 301 TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1528 LOCUS ADDGENE_1528 4762 bp DNA circular SYN 01/03/2005 DEFINITION L2963 complete plasmid sequence KEYWORDS . SOURCE L2963 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1528 at www.addgene.org FEATURES Location/Qualifiers source 1..4762 /organism="Addgene plasmid L2963" /mol_type="other DNA" CDS 1..804 /label="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLR" misc_feature 1..27 /label="SV40.NLS" gene 43..972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 286..305 /label="gfp.S65C" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 789..815 /label="SynIVSpi.5'ss" misc_feature 855..885 /label="SynIVSpi.3'ss" misc_feature 995..1045 /label="SynIVS.M" misc_feature 1122..1172 /label="SynIVS.L" promoter 2300..2328 /label="Amp_promoter" gene 2370..3230 /label="Ampicillin" /gene="Ampicillin" CDS 2370..3230 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3385..4004 /label="pBR322_origin" misc_feature 4220..4246 /label="lacI.C" promoter 4313..4342 /label="lac_promoter" misc_feature 4377..4395 /label="M13r_primer" misc_feature 4432..4457 /label="unc54.-204" misc_feature 4647..4670 /label="unc54.5'" misc_feature 4688..4729 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA 301 TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTATCTAAG gtaagtttaa atgcatggtg accaacgttg ctagcgctac 841 taactaactt gtactatcaa atttttcagC ACACAATCTG CCCTTTCCAA AGATCCCAAC 901 GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC 961 ATGGATGAAC TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac 1021 taacgcgctc tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA 1081 CTTGTCTGGT GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc 1141 tactaactaa cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA 1201 AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT 1261 TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC 1321 TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA 1381 AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG 1441 TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC 1501 GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT 1561 CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT 1621 TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT 1681 GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA 1741 AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG 1801 AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA 1861 ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG 1921 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 1981 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 2041 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT 2101 ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 2161 CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 2221 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 2281 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 2341 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 2401 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 2461 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 2521 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA 2581 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 2641 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 2701 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 2761 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 2821 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 2881 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 2941 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 3001 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 3061 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 3121 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 3181 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 3241 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 3301 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 3361 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 3421 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 3481 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 3541 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 3601 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 3661 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 3721 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 3781 TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 3841 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 3901 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 3961 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 4021 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 4081 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 4141 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 4201 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 4261 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 4321 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA 4381 ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCC 4441 CAATTCTTGA GACTTCCACT ATCTTCTCTG GCACCCTCCA CTACGCGTAG GCGTCTCTCG 4501 CTTCGTGTAT TCCCGGGAAG CCGGGTTCCC GTCTCTCCCG CCGCTGCCGC TGCCGCACAC 4561 AGCTTTACAC CTCGTAGAAT CCCCAAAGAG GGGCGTGGCT TGCGGGTGCC AACATCCTCC 4621 TGCCGAGGAA GAAGCAGGCA CTCATCACTC GCATCATCAA CCTCGGGATT GGCCAAAGGA 4681 CCCAAAGGTA TGTTTCGAAT GATACTAACA TAACATAGAA CATTTTCAGG AGGACCCTTG 4741 GAGGGTACCG AGCTCAGAAA AA // 1529 LOCUS ADDGENE_1529 4097 bp DNA circular SYN 01/03/2005 DEFINITION L3110 complete plasmid sequence KEYWORDS . SOURCE L3110 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1529 at www.addgene.org FEATURES Location/Qualifiers source 1..4097 /organism="Addgene plasmid L3110" /mol_type="other DNA" CDS 199..1071 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKR" gene 199..1059 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" promoter complement(1252..1270) /label="T7_promoter" misc_feature complement(1281..1297) /label="M13f20_primer" rep_origin 1456..1762 /label="f1_origin" promoter 1955..1983 /label="Amp_promoter" gene 2025..2885 /label="Ampicillin" /gene="Ampicillin" CDS 2025..2885 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3040..3659 /label="pBR322_origin" promoter 3968..3997 /label="lac_promoter" misc_feature 4032..4050 /label="M13r_primer" promoter 4067..4086 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TCGGTACCGG TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT 241 CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA 301 GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT 361 GTTCCATGGg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag 421 CCAACACTTG TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT 481 ATGAAACgGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT 541 ATATTTTTCA AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac 601 taaccataca tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT 661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA 721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA 781 ATCAAAGTTg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag 841 AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA 901 CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA 961 CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT 1021 GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAAAGgta agttttaaca 1081 tttaatcata cgaaatgaaa gagctccacc gcggtggcgg ccgctctaga actagtggat 1141 ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag gaattcgata 1201 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccCAATTC GCCCTATAGT 1261 GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 1321 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 1381 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC 1441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 1501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 1561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 1621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 1681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 1741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 1801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 1861 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC 1921 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 1981 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 2041 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2101 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2161 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 2221 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 2281 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 2341 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 2401 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 2461 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 2521 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 2581 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 2641 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2701 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2761 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2821 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 2881 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 2941 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3001 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3061 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3121 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3181 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3241 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3301 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3361 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3421 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3481 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3541 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3601 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3661 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 3721 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 3781 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 3841 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 3901 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 3961 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 4021 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT 4081 AAAGGGAACA AAAGCTG // 1530 LOCUS ADDGENE_1530 4097 bp DNA circular SYN 01/03/2005 DEFINITION L3111 complete plasmid sequence KEYWORDS . SOURCE L3111 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1530 at www.addgene.org FEATURES Location/Qualifiers source 1..4097 /organism="Addgene plasmid L3111" /mol_type="other DNA" CDS 200..1078 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKGKF" gene 200..1060 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 371..421 /label="SynIVS.R" misc_feature 443..462 /label="gfp.S65C" misc_feature 576..626 /label="SynIVS.S" misc_feature 791..841 /label="SynIVS.T" promoter complement(1252..1270) /label="T7_promoter" misc_feature 1290..1438 /label="lacZ_a" misc_feature complement(1281..1297) /label="M13f20_primer" rep_origin 1456..1762 /label="f1_origin" promoter 1955..1983 /label="Amp_promoter" CDS 2025..2885 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2025..2885 /label="Ampicillin" /gene="Ampicillin" rep_origin 3040..3659 /label="pBR322_origin" misc_feature 3875..3901 /label="lacI.C" promoter 3968..3997 /label="lac_promoter" misc_feature 4032..4050 /label="M13r_primer" promoter 4067..4086 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TCCGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 241 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 301 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 361 TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt taaattttca 421 gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCgAGAT ACCCAGATCA 481 TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC 541 TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa 601 ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT 661 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA 721 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG 781 AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca 841 gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA 901 ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC 961 ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT 1021 TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAAGgta agttttaaca 1081 tttaatcata cgaaatgaaa gagctccacc gcggtggcgg ccgctctaga actagtggat 1141 ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag gaattcgata 1201 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccCAATTC GCCCTATAGT 1261 GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 1321 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 1381 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC 1441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 1501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 1561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 1621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 1681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 1741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 1801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 1861 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC 1921 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 1981 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 2041 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2101 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2161 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 2221 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 2281 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 2341 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 2401 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 2461 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 2521 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 2581 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 2641 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2701 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2761 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2821 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 2881 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 2941 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3001 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3061 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3121 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3181 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3241 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3301 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3361 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3421 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3481 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3541 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3601 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3661 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 3721 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 3781 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 3841 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 3901 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 3961 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 4021 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT 4081 AAAGGGAACA AAAGCTG // 1531 LOCUS ADDGENE_1531 8492 bp DNA circular SYN 01/03/2005 DEFINITION L3135 complete plasmid sequence KEYWORDS . SOURCE L3135 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1531 at www.addgene.org FEATURES Location/Qualifiers source 1..8492 /organism="Addgene plasmid L3135" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" CDS 518..2113 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 518..544 /label="SV40.NLS" gene 560..1420 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 731..781 /label="SynIVS.R" misc_feature 803..822 /label="gfp.S65C" misc_feature 936..986 /label="SynIVS.S" misc_feature 1151..1201 /label="SynIVS.T" gene 1517..5070 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1651..1701 /label="SynIVS.B" CDS 2152..5073 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2329..2379 /label="SynIVS.D" misc_feature 2770..2820 /label="SynIVS.E" misc_feature 3077..3127 /label="SynIVS.F" misc_feature 3358..3408 /label="SynIVS.G" misc_feature 3645..3695 /label="SynIVS.H" misc_feature 4011..4061 /label="SynIVS.I" misc_feature 4322..4372 /label="SynIVS.J" misc_feature 4728..4778 /label="SynIVS.K" misc_feature 5094..5144 /label="SynIVS.L" promoter 6272..6300 /label="Amp_promoter" gene 6342..7202 /label="Ampicillin" /gene="Ampicillin" CDS 6342..7202 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7357..7976 /label="pBR322_origin" misc_feature 8192..8218 /label="lacI.C" promoter 8285..8314 /label="lac_promoter" misc_feature 8349..8367 /label="M13r_primer" misc_feature 8381..8431 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 601 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 661 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 721 TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt taaattttca 781 gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCgAGAT ACCCAGATCA 841 TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC 901 TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa 961 ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT 1021 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA 1081 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG 1141 AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca 1201 gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA 1261 ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC 1321 ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT 1381 TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATgcC CGGTGGGTGA 1441 AGACCAGAAA CAGCACCTCG AACTGAGCCG CGATATTGCC CAGCGTTTCA ACGCGCTGTA 1501 TGGCGAGATC GATCCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA 1561 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG 1621 CACCGATCGC CCTTCCCAAC AGTTGCGaAG gtaagtttaa acagatccat actaactaac 1681 ttgttctgac ataattttca gCtTGAATGG CGAATGGCGC TTTGCCTGGT TTCCGGCACC 1741 AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA CTGTCGTCGT 1801 CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG TGACCTATCC 1861 CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT ACTCGCTCAC 1921 ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT TTGATGGCGT 1981 TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC GGTTACGGCC AGgtaagttt 2041 aattaagttg ataCTAACTA ACAAGATCTG ATTAATTTTC agGACAGTCG TTTGCCGTCT 2101 GAATTTGACC TGAGCGCATT TTTACGCGCC GGAGAAAACC GCCTCGCGGT GATGGTGCTG 2161 CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT CAGGATATGT GGCGGATGAG CGGCATTTTC 2221 CGTGACGTCT CGTTGCTGCA TAAACCGACT ACACAAATCA GCGATTTCCA TGTTGCCACT 2281 CGCTTTAATG ATGATTTCtc cCGCGCTGTA CTGGAGGCTG AAGTcCAGgt aagtttaaac 2341 aggatcttac taactaacat gctaacactg aattttcagA TGTGCGGCGA GTTGCGTGAC 2401 TACCTACGGG TAACAGTTTC TTTATGGCAG GGTGAAACGC AGGTCGCCAG CGGCACCGCG 2461 CCTTTCGGCG GTGAAATTAT CGATGAGCGT GGTGGTTATG CCGATCGCGT CACACTACGT 2521 CTGAACGTCG AAAACCCGAA ACTGTGGAGC GCCGAAATCC CGAATCTCTA TCGTGCGGTG 2581 GTTGAACTGC ACACCGCCGA CGGCACGCTG ATTGAAGCAG AAGCCTGCGA TGTCGGTTTC 2641 CGCGAGGTGC GGATTGAAAA TGGTCTGCTG CTGCTGAACG GCAAGCCGTT GCTGATTCGA 2701 GGCGTTAACC GTCACGAGCA TCATCCTCTG CATGGTCAGG TCATGGATGA GCAGACGATG 2761 GTGCAGGATg taagtttaaa ctattcgtta ctaactaact ttaaacattt aaattttcag 2821 ATCCTGCTGA TGAAGCAGAA CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT 2881 CCGCTGTGGT ACACGCTGTG CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT 2941 GAAACCCACG GCATGGTGCC AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG 3001 ATGAGCGAAC GCGTAACGCG AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC 3061 TGGTCGCTGG GGAATGgtaa gtttaaacag ttgaatacta actaacggag atctttgaaa 3121 ttttcagAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 3181 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 3241 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 3301 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATtCT TTGCGAGgta 3361 agtttaaaca gaactctact aactaacaca ttagatccta attttcagTA CGCtCACGCG 3421 ATGGGcAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT 3481 TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC 3541 GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC 3601 TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCgtaagt ttaaacaata 3661 acctactaac taacgtagat aatttaaatt ttcagGCTGA CGGAAGCAAA ACACCAGCAG 3721 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 3781 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 3841 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 3901 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 3961 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCaTGGC AGCAGTGGAG gtaagtttaa 4021 acaagatcct actaactaac tctacattga tgaattttca gACTGGCGGA AAACCTCAGT 4081 GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA AATGGATTTT 4141 TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT TCTTTCACAG 4201 ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT CACCCGTGCA 4261 CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA CGCCTGGGTC 4321 Ggtaagttta aacaaagttg tactaactaa cgaagatctt gataattttc agAACGCTGG 4381 AAGGCGGCGG GCCATTACCA GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT 4441 GCTGATGCGG TGCTGATTAC GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT 4501 ATCAGCCGGA AAACCTACCG GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT 4561 GAAGTGGCGA GCGATACACC GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG 4621 GTAGCAGAGC GGGTAAACTG GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT 4681 ACTGCCGCCT GTTTTGACCG CTGGGATCTG CCATTGTCAG ACATGTAgta agtttaaact 4741 tgatagtact aactaacatg tttcatttaa attttcagTA CCCCGTACGT CTTCCCGAGC 4801 GAAAACGGTC TGCGCTGCGG GACGCGCGAA TTGAATTATG GCCCACACCA GTGGCGCGGC 4861 GACTTCCAGT TCAACATCAG CCGCTACAGT CAACAGCAAC TGATGGAAAC CAGCCATCGC 4921 CATCTGCTGC ACGCGGAAGA AGGCACATGG CTGAATATCG ACGGTTTCCA TATGGGGATT 4981 GGTGGCGACG ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT TCCAACTGAG CGCCGGTCGC 5041 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 5101 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 5161 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 5221 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 5281 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 5341 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 5401 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 5461 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 5521 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 5581 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 5641 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 5701 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 5761 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 5821 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 5881 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 5941 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 6001 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 6061 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 6121 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 6181 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 6241 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 6301 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 6361 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 6421 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 6481 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 6541 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 6601 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 6661 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 6721 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 6781 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 6841 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 6901 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 6961 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 7021 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 7081 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 7141 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 7201 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 7261 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 7321 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 7381 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 7441 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 7501 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 7561 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 7621 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 7681 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 7741 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 7801 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 7861 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 7921 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 7981 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 8041 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 8101 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 8161 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 8221 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 8281 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 8341 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 8401 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 8461 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1532 LOCUS ADDGENE_1532 8272 bp DNA circular SYN 01/03/2005 DEFINITION L3136 complete plasmid sequence KEYWORDS . SOURCE L3136 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1532 at www.addgene.org FEATURES Location/Qualifiers source 1..8272 /organism="Addgene plasmid L3136" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" misc_feature 298..324 /label="SV40.NLS" CDS 298..1893 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 340..1200 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 511..561 /label="SynIVS.R" misc_feature 583..602 /label="gfp.S65C" misc_feature 716..766 /label="SynIVS.S" misc_feature 931..981 /label="SynIVS.T" gene 1297..4850 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1431..1481 /label="SynIVS.B" CDS 1932..4853 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2109..2159 /label="SynIVS.D" misc_feature 2550..2600 /label="SynIVS.E" misc_feature 2857..2907 /label="SynIVS.F" misc_feature 3138..3188 /label="SynIVS.G" misc_feature 3425..3475 /label="SynIVS.H" misc_feature 3791..3841 /label="SynIVS.I" misc_feature 4102..4152 /label="SynIVS.J" misc_feature 4508..4558 /label="SynIVS.K" misc_feature 4874..4924 /label="SynIVS.L" promoter 6052..6080 /label="Amp_promoter" gene 6122..6982 /label="Ampicillin" /gene="Ampicillin" CDS 6122..6982 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7137..7756 /label="pBR322_origin" misc_feature 7972..7998 /label="lacI.C" promoter 8065..8094 /label="lac_promoter" misc_feature 8129..8147 /label="M13r_primer" misc_feature 8161..8211 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT 361 TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT 421 TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT 481 TGCACTACTG GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac 541 cctgattatt taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC 601 TTcTCgAGAT ACCCAGATCA TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA 661 GGTTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag 721 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG 781 TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT 841 GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG 901 GCAGACAAAC AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac 961 taatctgatt taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT 1021 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA 1081 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC 1141 CACATGGTCC TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA 1201 TACAAATgcC CGGTGGGTGA AGACCAGAAA CAGCACCTCG AACTGAGCCG CGATATTGCC 1261 CAGCGTTTCA ACGCGCTGTA TGGCGAGATC GATCCCGTCG TTTTACAACG TCGTGACTGG 1321 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 1381 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGaAG gtaagtttaa 1441 acagatccat actaactaac ttgttctgac ataattttca gCtTGAATGG CGAATGGCGC 1501 TTTGCCTGGT TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT 1561 GAGGCCGATA CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC 1621 TACACCAACG TGACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG 1681 ACGGGTTGTT ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG 1741 CGAATTATTT TTGATGGCGT TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC 1801 GGTTACGGCC AGgtaagttt aattaagttg ataCTAACTA ACAAGATCTG ATTAATTTTC 1861 agGACAGTCG TTTGCCGTCT GAATTTGACC TGAGCGCATT TTTACGCGCC GGAGAAAACC 1921 GCCTCGCGGT GATGGTGCTG CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT CAGGATATGT 1981 GGCGGATGAG CGGCATTTTC CGTGACGTCT CGTTGCTGCA TAAACCGACT ACACAAATCA 2041 GCGATTTCCA TGTTGCCACT CGCTTTAATG ATGATTTCtc cCGCGCTGTA CTGGAGGCTG 2101 AAGTcCAGgt aagtttaaac aggatcttac taactaacat gctaacactg aattttcagA 2161 TGTGCGGCGA GTTGCGTGAC TACCTACGGG TAACAGTTTC TTTATGGCAG GGTGAAACGC 2221 AGGTCGCCAG CGGCACCGCG CCTTTCGGCG GTGAAATTAT CGATGAGCGT GGTGGTTATG 2281 CCGATCGCGT CACACTACGT CTGAACGTCG AAAACCCGAA ACTGTGGAGC GCCGAAATCC 2341 CGAATCTCTA TCGTGCGGTG GTTGAACTGC ACACCGCCGA CGGCACGCTG ATTGAAGCAG 2401 AAGCCTGCGA TGTCGGTTTC CGCGAGGTGC GGATTGAAAA TGGTCTGCTG CTGCTGAACG 2461 GCAAGCCGTT GCTGATTCGA GGCGTTAACC GTCACGAGCA TCATCCTCTG CATGGTCAGG 2521 TCATGGATGA GCAGACGATG GTGCAGGATg taagtttaaa ctattcgtta ctaactaact 2581 ttaaacattt aaattttcag ATCCTGCTGA TGAAGCAGAA CAACTTTAAC GCCGTGCGCT 2641 GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG CGACCGCTAC GGCCTGTATG 2701 TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC AATGAATCGT CTGACCGATG 2761 ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG AATGGTGCAG CGCGATCGTA 2821 ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGgtaa gtttaaacag ttgaatacta 2881 actaacggag atctttgaaa ttttcagAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT 2941 ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG 3001 ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT 3061 TCCCGGCTGT GCCGAAATGG TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC 3121 CGCTGATtCT TTGCGAGgta agtttaaaca gaactctact aactaacaca ttagatccta 3181 attttcagTA CGCtCACGCG ATGGGcAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG 3241 CGTTTCGTCA GTATCCCCGT TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC 3301 TGATTAAATA TGATGAAAAC GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA 3361 CGCCGAACGA TCGCCAGTTC TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC 3421 CAGCgtaagt ttaaacaata acctactaac taacgtagat aatttaaatt ttcagGCTGA 3481 CGGAAGCAAA ACACCAGCAG CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG 3541 TGACCAGCGA ATACCTGTTC CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC 3601 TGGATGGTAA GCCGCTGGCA AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC 3661 AGTTGATTGA ACTGCCTGAA CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG 3721 TACGCGTAGT GCAACCGAAC GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCaTGGC 3781 AGCAGTGGAG gtaagtttaa acaagatcct actaactaac tctacattga tgaattttca 3841 gACTGGCGGA AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA 3901 CCACCAGCGA AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC 3961 AGTCAGGCTT TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC 4021 GCGATCAGTT CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA 4081 TTGACCCTAA CGCCTGGGTC Ggtaagttta aacaaagttg tactaactaa cgaagatctt 4141 gataattttc agAACGCTGG AAGGCGGCGG GCCATTACCA GGCCGAAGCA GCGTTGTTGC 4201 AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC GACCGCTCAC GCGTGGCAGC 4261 ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG GATTGATGGT AGTGGTCAAA 4321 TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC GCATCCGGCG CGGATTGGCC 4381 TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG GCTCGGATTA GGGCCGCAAG 4441 AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG CTGGGATCTG CCATTGTCAG 4501 ACATGTAgta agtttaaact tgatagtact aactaacatg tttcatttaa attttcagTA 4561 CCCCGTACGT CTTCCCGAGC GAAAACGGTC TGCGCTGCGG GACGCGCGAA TTGAATTATG 4621 GCCCACACCA GTGGCGCGGC GACTTCCAGT TCAACATCAG CCGCTACAGT CAACAGCAAC 4681 TGATGGAAAC CAGCCATCGC CATCTGCTGC ACGCGGAAGA AGGCACATGG CTGAATATCG 4741 ACGGTTTCCA TATGGGGATT GGTGGCGACG ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT 4801 TCCAACTGAG CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC 4861 CGCTGTCATC AGAgtaagtt taaactgagt tctactaact aacgagtaat atttaaattt 4921 tcagCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA 4981 TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA 5041 TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA 5101 AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA 5161 TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG 5221 ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC 5281 GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA 5341 ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT 5401 TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT 5461 TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA 5521 TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA 5581 GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA 5641 ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT 5701 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG 5761 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC 5821 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA 5881 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT 5941 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC 6001 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT 6061 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG 6121 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC 6181 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 6241 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC 6301 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC 6361 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT 6421 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT 6481 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT 6541 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT 6601 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT 6661 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC 6721 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 6781 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 6841 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA 6901 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC 6961 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA 7021 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 7081 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 7141 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 7201 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 7261 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT 7321 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 7381 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 7441 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 7501 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC 7561 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 7621 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 7681 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 7741 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 7801 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 7861 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA 7921 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 7981 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 8041 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 8101 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT 8161 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gAGCTTAAAA 8221 ATGGCTGAAA TCACTCACAA CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1533 LOCUS ADDGENE_1533 9720 bp DNA circular SYN 01/03/2005 DEFINITION L3137 complete plasmid sequence KEYWORDS . SOURCE L3137 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1533 at www.addgene.org FEATURES Location/Qualifiers source 1..9720 /organism="Addgene plasmid L3137" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" CDS 1746..3341 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 1746..1772 /label="SV40.NLS" gene 1788..2648 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1959..2009 /label="SynIVS.R" misc_feature 2031..2050 /label="gfp.S65C" misc_feature 2164..2214 /label="SynIVS.S" misc_feature 2379..2429 /label="SynIVS.T" gene 2745..6298 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 2879..2929 /label="SynIVS.B" CDS 3380..6301 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 3557..3607 /label="SynIVS.D" misc_feature 3998..4048 /label="SynIVS.E" misc_feature 4305..4355 /label="SynIVS.F" misc_feature 4586..4636 /label="SynIVS.G" misc_feature 4873..4923 /label="SynIVS.H" misc_feature 5239..5289 /label="SynIVS.I" misc_feature 5550..5600 /label="SynIVS.J" misc_feature 5956..6006 /label="SynIVS.K" misc_feature 6322..6372 /label="SynIVS.L" promoter 7500..7528 /label="Amp_promoter" gene 7570..8430 /label="Ampicillin" /gene="Ampicillin" CDS 7570..8430 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 8585..9204 /label="pBR322_origin" misc_feature 9420..9446 /label="lacI.C" promoter 9513..9542 /label="lac_promoter" misc_feature 9577..9595 /label="M13r_primer" misc_feature 9609..9659 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG AGTAAAGGAG 1801 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 1861 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 1921 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac 1981 taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG 2041 TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA 2101 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 2161 CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG 2221 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 2281 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 2341 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac 2401 taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG 2461 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 2521 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 2581 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 2641 ATGAACTATA CAAATgcCCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG 2701 ATATTGCCCA GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC 2761 GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG 2821 CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGaAGgt 2881 aagtttaaac agatccatac taactaactt gttctgacat aattttcagC tTGAATGGCG 2941 AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG 3001 ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG 3061 CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG 3121 AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG 3181 GCCAGACGCG AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG TGCAACGGGC 3241 GCTGGGTCGG TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC AAGATCTGAT 3301 TAATTTTCag GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT TACGCGCCGG 3361 AGAAAACCGC CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC TGGAAGATCA 3421 GGATATGTGG CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA AACCGACTAC 3481 ACAAATCAGC GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC GCGCTGTACT 3541 GGAGGCTGAA GTcCAGgtaa gtttaaacag gatcttacta actaacatgc taacactgaa 3601 ttttcagATG TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG 3661 TGAAACGCAG GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG ATGAGCGTGG 3721 TGGTTATGCC GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC TGTGGAGCGC 3781 CGAAATCCCG AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG GCACGCTGAT 3841 TGAAGCAGAA GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG GTCTGCTGCT 3901 GCTGAACGGC AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC ATCCTCTGCA 3961 TGGTCAGGTC ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact attcgttact 4021 aactaacttt aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA ACTTTAACGC 4081 CGTGCGCTGT TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG ACCGCTACGG 4141 CCTGTATGTG GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA TGAATCGTCT 4201 GACCGATGAT CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA TGGTGCAGCG 4261 CGATCGTAAT CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt ttaaacagtt 4321 gaatactaac taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG CTAATCACGA 4381 CGCGCTGTAT CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG 4441 CGGAGCCGAC ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA 4501 CCAGCCCTTC CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA 4561 GACGCGCCCG CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa ctaacacatt 4621 agatcctaat tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT TCGCTAAATA 4681 CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA 4741 TCAGTCGCTG ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT 4801 TGGCGATACG CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC 4861 GCCGCATCCA GCgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt 4921 cagGCTGACG GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC 4981 CATCGAAGTG ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT 5041 GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA 5101 AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG 5161 GCTCACAGTA CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG 5221 CGCaTGGCAG CAGTGGAGgt aagtttaaac aagatcctac taactaactc tacattgatg 5281 aattttcagA CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC 5341 GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT 5401 TAACCGCCAG TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC 5461 GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC 5521 GACCCGCATT GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta ctaactaacg 5581 aagatcttga taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC 5641 GTTGTTGCAG TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC 5701 GTGGCAGCAT CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG 5761 TGGTCAAATG GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG 5821 GATTGGCCTG AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG 5881 GCCGCAAGAA AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC 5941 ATTGTCAGAC ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat 6001 tttcagTACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT 6061 GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA 6121 ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT 6181 GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC 6241 GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA 6301 ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat 6361 ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA 6421 TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT 6481 TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA 6541 GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC 6601 ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT 6661 TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA 6721 TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT 6781 GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA 6841 GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG 6901 TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC 6961 AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA 7021 ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA 7081 TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT 7141 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 7201 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 7261 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 7321 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC 7381 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 7441 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 7501 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 7561 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 7621 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 7681 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 7741 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 7801 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 7861 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 7921 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 7981 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 8041 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 8101 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 8161 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 8221 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 8281 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 8341 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 8401 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 8461 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 8521 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 8581 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 8641 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 8701 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 8761 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 8821 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 8881 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 8941 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 9001 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 9061 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 9121 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 9181 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 9241 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 9301 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 9361 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 9421 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 9481 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 9541 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC 9601 GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA 9661 GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1534 LOCUS ADDGENE_1534 3634 bp DNA circular SYN 01/03/2005 DEFINITION L3465 complete plasmid sequence KEYWORDS . SOURCE L3465 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1534 at www.addgene.org FEATURES Location/Qualifiers source 1..3634 /organism="Addgene plasmid L3465" /mol_type="other DNA" CDS 38..1168 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTSRDDASPWGIQVPVEKMSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYG VQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFS GAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSL NMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL SKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQALHVPLMTSRDDASPWGIHWPSFY NVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA" gene 92..940 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 263..313 /label="SynIVS.R" misc_feature 314..333 /label="gfp.F64L" misc_feature 335..354 /label="gfp.S65T" misc_feature 468..518 /label="SynIVS.S" misc_feature 683..733 /label="SynIVS.T" misc_feature 1020..1175 /label="lacZ_a" misc_feature complement(1023..1039) /label="M13f20_primer" promoter 1548..1576 /label="Amp_promoter" CDS 1618..2478 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1618..2478 /label="Ampicillin" /gene="Ampicillin" rep_origin 2633..3252 /label="pBR322_origin" misc_feature 3468..3494 /label="lacI.C" promoter 3561..3590 /label="lac_promoter" misc_feature 3625..3643 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag ctttacatgt cccattaatg acgtcccggg atgacgcgtc 61 gccatggggA ATTCAAGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG 121 AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG 181 TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC 241 TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta accctgatta 301 tttaaatttt cagCCAACAC TTGTCACTAC TCTCACTTAT GGTGTTCAAT GCTTCTCGAG 361 ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT 421 ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca 481 gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG 541 TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT 601 TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA 661 ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta actaatctga 721 tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC 781 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA 841 TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT 901 CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGAcGAAC TATACAAAag 961 cccacaagct ttacatgtcc cattaatgac gtcccgggat gacgcgtcgc catggggaat 1021 tcACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 1081 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 1141 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC 1201 CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT 1261 GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG 1321 GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG 1381 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC 1441 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 1501 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 1561 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 1621 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 1681 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 1741 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 1801 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 1861 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 1921 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 1981 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 2041 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 2101 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 2161 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 2221 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 2281 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 2341 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 2401 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 2461 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 2521 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 2581 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 2641 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 2701 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 2761 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 2821 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 2881 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 2941 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 3001 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT 3061 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 3121 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 3181 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 3241 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 3301 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 3361 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 3421 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 3481 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC 3541 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT 3601 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1535 LOCUS ADDGENE_1535 3646 bp DNA circular SYN 01/03/2005 DEFINITION L3467 complete plasmid sequence KEYWORDS . SOURCE L3467 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1535 at www.addgene.org FEATURES Location/Qualifiers source 1..3646 /organism="Addgene plasmid L3467" /mol_type="other DNA" CDS 62..1180 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSRQGIQVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGE GEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSR YPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKF EGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLT N*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE KRDHMVLLEFVTAAGITHGMDELYKSPQASGPELDVPRINIIMSRQGIHWPSFYNVVT GKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA" gene 98..946 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 269..319 /label="SynIVS.R" misc_feature 320..339 /label="gfp.F64L" misc_feature 341..360 /label="gfp.S65T" misc_feature 474..524 /label="SynIVS.S" misc_feature 689..739 /label="SynIVS.T" misc_feature 1032..1187 /label="lacZ_a" misc_feature complement(1035..1051) /label="M13f20_primer" promoter 1560..1588 /label="Amp_promoter" CDS 1630..2490 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1630..2490 /label="Ampicillin" /gene="Ampicillin" rep_origin 2645..3264 /label="pBR322_origin" misc_feature 3480..3506 /label="lacI.C" promoter 3573..3602 /label="lac_promoter" misc_feature 3637..3655 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag cttcagggcc cgagctcgac gtcccgcgga ttaatataat 61 tatgagtcga caaggAATTC AAGTACCGGT AGAAAAAATG AGTAAAGGAG AAGAACTTTT 121 CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC 181 TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG 241 CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac taactaaccc 301 tgattattta aattttcagC CAACACTTGT CACTACTCTC ACTTATGGTG TTCAATGCTT 361 CTCGAGATAC CCAGATCATA TGAAACAGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG 421 TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA CACgtaagtt 481 taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG AAGTCAAGTT 541 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG 601 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC 661 AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac taactaacta 721 atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA 781 ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA 841 CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA 901 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG AcGAACTATA 961 CAAAagccca caagcttcag ggcccgagct cgacgtcccg cggattaata taattatgag 1021 tcgacaagga attcACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 1081 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 1141 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG 1201 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT 1261 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC 1321 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC 1381 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC 1441 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 1501 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 1561 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 1621 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 1681 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 1741 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 1801 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 1861 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 1921 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 1981 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 2041 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 2101 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 2161 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 2221 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 2281 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 2341 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 2401 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 2461 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 2521 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 2581 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 2641 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 2701 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 2761 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 2821 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 2881 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 2941 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 3001 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 3061 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 3121 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 3181 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 3241 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 3301 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 3361 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 3421 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 3481 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 3541 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 3601 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1536 LOCUS ADDGENE_1536 3616 bp DNA circular SYN 01/03/2005 DEFINITION L3469 complete plasmid sequence KEYWORDS . SOURCE L3469 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1536 at www.addgene.org FEATURES Location/Qualifiers source 1..3616 /organism="Addgene plasmid L3469" /mol_type="other DNA" CDS 83..1150 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQAFTRPGTAAALKLGIHWPSFYNVVTGKTLALPNLIALQHIPL SPAGVIAKRPAPIALPNSCAA" gene 83..931 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 254..304 /label="SynIVS.R" misc_feature 305..324 /label="gfp.F64L" misc_feature 326..345 /label="gfp.S65T" misc_feature 459..509 /label="SynIVS.S" misc_feature 674..724 /label="SynIVS.T" misc_feature 1002..1157 /label="lacZ_a" misc_feature complement(1005..1021) /label="M13f20_primer" promoter 1530..1558 /label="Amp_promoter" CDS 1600..2460 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1600..2460 /label="Ampicillin" /gene="Ampicillin" rep_origin 2615..3234 /label="pBR322_origin" misc_feature 3450..3476 /label="lacI.C" promoter 3543..3572 /label="lac_promoter" misc_feature 3607..3625 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag ctttcacgcg tcctggtacc gctgcagcgc ttaagttggg 61 AATTCAAGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC 121 AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG 181 TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT 241 ACCTGTTCCA TGGgtaagtt taaacatata tatactaact aaccctgatt atttaaattt 301 tcagCCAACA CTTGTCACTA CTCTCACTTA TGGTGTTCAA TGCTTCTCGA GATACCCAGA 361 TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG 421 AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac 481 taactaacca tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT 541 TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA 601 CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA 661 TGGAATCAAA GTTgtaagtt taaacatgat tttactaact aactaatctg atttaaattt 721 tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA 781 TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC 841 CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA 901 GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGAcGAA CTATACAAAa gcccacaagc 961 tttcacgcgt cctggtaccg ctgcagcgct taagttggga attcACTGGC CGTCGTTTTA 1021 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC 1081 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG 1141 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT 1201 ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 1261 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 1321 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 1381 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 1441 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 1501 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 1561 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 1621 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 1681 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 1741 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 1801 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 1861 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 1921 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 1981 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2041 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 2101 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 2161 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 2221 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 2281 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 2341 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 2401 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 2461 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 2521 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 2581 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 2641 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 2701 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 2761 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 2821 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 2881 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 2941 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3001 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3061 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 3121 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 3181 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 3241 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 3301 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 3361 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 3421 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 3481 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 3541 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 3601 CACACAGGAA ACAGCT // 1537 LOCUS ADDGENE_1537 3700 bp DNA circular SYN 01/03/2005 DEFINITION L3471 complete plasmid sequence KEYWORDS . SOURCE L3471 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1537 at www.addgene.org FEATURES Location/Qualifiers source 1..3700 /organism="Addgene plasmid L3471" /mol_type="other DNA" CDS 26..1234 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSWAHRFGRPEGLSGRVEGIPGYRARIQVPVEKMSKGEELFTGV VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPD YLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRK FKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVY IMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGP VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQAYMSWAHRF GRPEGLSGRVEGIPGYRARIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPA PIALPNSCAA" gene 125..973 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 296..346 /label="SynIVS.R" misc_feature 347..366 /label="gfp.F64L" misc_feature 368..387 /label="gfp.S65T" misc_feature 501..551 /label="SynIVS.S" misc_feature 716..766 /label="SynIVS.T" misc_feature 1086..1241 /label="lacZ_a" misc_feature complement(1089..1105) /label="M13f20_primer" promoter 1614..1642 /label="Amp_promoter" CDS 1684..2544 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1684..2544 /label="Ampicillin" /gene="Ampicillin" rep_origin 2699..3318 /label="pBR322_origin" misc_feature 3534..3560 /label="lacI.C" promoter 3627..3656 /label="lac_promoter" misc_feature 3691..3709 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag cttacatgtc ctgggcccat cgattcggac gtcccgaggg 61 cttaagcgga cgcgtcgagg ggatccccgg gtaccgagct cgAATTCAAG TACCGGTAGA 121 AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA 181 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA 241 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag 301 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC 361 TACTCTCACT TATGGTGTTC AATGCTTCTC GAGATACCCA GATCATATGA AACAGCATGA 421 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 481 TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt 541 taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT 601 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA 661 TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag 721 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG 781 ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT 841 TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC 901 GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG 961 GATTACACAT GGCATGGAcG AACTATACAA Aagcccacaa gcttacatgt cctgggccca 1021 tcgattcgga cgtcccgagg gcttaagcgg acgcgtcgag gggatccccg ggtaccgagc 1081 tcgaattcAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA 1141 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC 1201 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT 1261 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC 1321 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC 1381 TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC 1441 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG 1501 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 1561 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 1621 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 1681 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 1741 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 1801 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 1861 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 1921 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 1981 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 2041 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 2101 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 2161 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 2221 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 2281 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 2341 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 2401 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 2461 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 2521 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 2581 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 2641 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 2701 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 2761 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 2821 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 2881 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 2941 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 3001 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 3061 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 3121 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 3181 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 3241 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 3301 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 3361 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 3421 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 3481 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 3541 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 3601 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 3661 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1538 LOCUS ADDGENE_1538 4091 bp DNA circular SYN 01/03/2005 DEFINITION L3502 complete plasmid sequence KEYWORDS . SOURCE L3502 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1538 at www.addgene.org FEATURES Location/Qualifiers source 1..4091 /organism="Addgene plasmid L3502" /mol_type="other DNA" CDS 195..1079 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKVSFNI" gene 195..1055 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 366..416 /label="SynIVS.R" misc_feature 417..436 /label="gfp.F64L" misc_feature 438..457 /label="gfp.S65T" misc_feature 571..621 /label="SynIVS.S" misc_feature 786..836 /label="SynIVS.T" promoter complement(1246..1264) /label="T7_promoter" misc_feature 1284..1432 /label="lacZ_a" misc_feature complement(1275..1291) /label="M13f20_primer" rep_origin 1450..1756 /label="f1_origin" promoter 1949..1977 /label="Amp_promoter" gene 2019..2879 /label="Ampicillin" /gene="Ampicillin" CDS 2019..2879 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3034..3653 /label="pBR322_origin" misc_feature 3869..3895 /label="lacI.C" promoter 3962..3991 /label="lac_promoter" misc_feature 4026..4044 /label="M13r_primer" promoter 4061..4080 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 241 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 301 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 361 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 421 CACTTGTCAC TACTCTCACT TATGGTGTTC AATGCTTCTC GAGATACCCA GATCATATGA 481 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 541 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 601 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 661 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 721 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 781 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 841 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 901 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 961 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1021 CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA Agtaagtttt aacatttaat 1081 catacgaaat gaaagagctc caccgcggtg gcggccgctc tagaactagt ggatccaccg 1141 gttccatggc tagccacgtg acgcgtggat cccccgggct gcaggaattc gatatcaagc 1201 ttatcgatac cgtcgacctc gagggggggc ccggtaccCA ATTCGCCCTA TAGTGAGTCG 1261 TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 1321 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 1381 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC 1441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 1501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 1561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 1621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 1681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 1741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 1801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 1861 TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 1921 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 1981 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 2041 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 2101 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 2161 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 2221 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 2281 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 2341 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 2401 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 2461 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 2521 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 2581 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 2641 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 2701 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 2761 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 2821 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 2881 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 2941 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 3001 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 3061 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 3121 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 3181 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 3241 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 3301 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 3361 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 3421 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 3481 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 3541 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 3601 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 3661 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 3721 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 3781 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 3841 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 3901 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 3961 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 4021 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG 4081 AACAAAAGCT G // 1539 LOCUS ADDGENE_1539 4097 bp DNA circular SYN 01/03/2005 DEFINITION L3503 complete plasmid sequence KEYWORDS . SOURCE L3503 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1539 at www.addgene.org FEATURES Location/Qualifiers source 1..4097 /organism="Addgene plasmid L3503" /mol_type="other DNA" gene 200..1060 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 200..1078 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKGKF" misc_feature 371..421 /label="SynIVS.R" misc_feature 422..441 /label="gfp.F64L" misc_feature 443..462 /label="gfp.S65T" misc_feature 576..626 /label="SynIVS.S" misc_feature 791..841 /label="SynIVS.T" promoter complement(1252..1270) /label="T7_promoter" misc_feature 1290..1438 /label="lacZ_a" misc_feature complement(1281..1297) /label="M13f20_primer" rep_origin 1456..1762 /label="f1_origin" promoter 1955..1983 /label="Amp_promoter" gene 2025..2885 /label="Ampicillin" /gene="Ampicillin" CDS 2025..2885 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3040..3659 /label="pBR322_origin" misc_feature 3875..3901 /label="lacI.C" promoter 3968..3997 /label="lac_promoter" misc_feature 4032..4050 /label="M13r_primer" promoter 4067..4086 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TCCGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 241 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 301 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 361 TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt taaattttca 421 gCCAACACTT GTCACTACTC TCACTTATGG TGTTCAATGC TTCTCGAGAT ACCCAGATCA 481 TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC 541 TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa 601 ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT 661 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA 721 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG 781 AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca 841 gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA 901 ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC 961 ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT 1021 TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAAGgta agttttaaca 1081 tttaatcata cgaaatgaaa gagctccacc gcggtggcgg ccgctctaga actagtggat 1141 ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag gaattcgata 1201 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccCAATTC GCCCTATAGT 1261 GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 1321 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 1381 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC 1441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 1501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 1561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 1621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 1681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 1741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 1801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 1861 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC 1921 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 1981 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 2041 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2101 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2161 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 2221 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 2281 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 2341 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 2401 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 2461 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 2521 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 2581 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 2641 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2701 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2761 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2821 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 2881 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 2941 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3001 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3061 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3121 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3181 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3241 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3301 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3361 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3421 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3481 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3541 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3601 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3661 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 3721 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 3781 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 3841 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 3901 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 3961 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 4021 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT 4081 AAAGGGAACA AAAGCTG // 1540 LOCUS ADDGENE_1540 3963 bp DNA circular SYN 01/03/2005 DEFINITION L3505 complete plasmid sequence KEYWORDS . SOURCE L3505 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1540 at www.addgene.org FEATURES Location/Qualifiers source 1..3963 /organism="Addgene plasmid L3505" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1608 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPGDRWSSTAVAAALELVDPPGCRNSISSLSIPSTSRGGPVPNSP YSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLP LGFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 205..1065 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 376..426 /label="SynIVS.R" misc_feature 427..446 /label="gfp.F64L" misc_feature 448..467 /label="gfp.S65T" misc_feature 581..631 /label="SynIVS.S" misc_feature 796..846 /label="SynIVS.T" promoter complement(1204..1222) /label="T7_promoter" misc_feature 1242..1390 /label="lacZ_a" misc_feature complement(1233..1249) /label="M13f20_primer" rep_origin 1408..1714 /label="f1_origin" promoter 1907..1935 /label="Amp_promoter" gene 1977..2837 /label="Ampicillin" /gene="Ampicillin" CDS 1977..2837 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2992..3611 /label="pBR322_origin" misc_feature 3827..3853 /label="lacI.C" promoter 3920..3949 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC TCTAGAACTA 121 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG 181 GGGGGCCCGG TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC 241 CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG 301 GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA 361 CTACCTGTTC CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat 421 tttcagCCAA CACTTGTCAC TACTCTCACT TATGGTGTTC AATGCTTCTC GAGATACCCA 481 GATCATATGA AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA 541 AGAACTATAT TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt 601 actaactaac catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC 661 CTTGTTAATA GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA 721 CACAAATTGG AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG 781 AATGGAATCA AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat 841 tttcagAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT 1021 GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA ATgcccgggg 1081 gatcggtgga gctcCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC 1141 AGGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT 1201 TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC 1261 TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC 1321 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 1381 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 1441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 1501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 1561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 1621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 1681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 1741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 1801 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC 1861 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 1921 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 1981 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2041 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2101 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2161 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2221 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2281 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2341 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2401 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2461 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2521 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2581 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 2641 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2701 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 2761 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 2821 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 2881 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 2941 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3001 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3061 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3121 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3181 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3241 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3301 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3361 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 3421 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3481 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3541 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3601 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 3661 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 3721 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 3781 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 3841 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 3901 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 3961 TGA // 1541 LOCUS ADDGENE_1541 3957 bp DNA circular SYN 01/03/2005 DEFINITION L3506 complete plasmid sequence KEYWORDS . SOURCE L3506 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1541 at www.addgene.org FEATURES Location/Qualifiers source 1..3957 /organism="Addgene plasmid L3506" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" promoter 56..75 /label="T3_promoter" CDS 201..1205 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPGDRWSSTGGGRSRTSGSPGLQEFDIKLIDTVDLEGGPGTQFAL" gene 201..1061 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 372..422 /label="SynIVS.R" misc_feature 423..442 /label="gfp.F64L" misc_feature 444..463 /label="gfp.S65T" misc_feature 577..627 /label="SynIVS.S" misc_feature 792..842 /label="SynIVS.T" promoter complement(1198..1216) /label="T7_promoter" misc_feature 1236..1384 /label="lacZ_a" misc_feature complement(1227..1243) /label="M13f20_primer" rep_origin 1402..1708 /label="f1_origin" promoter 1901..1929 /label="Amp_promoter" gene 1971..2831 /label="Ampicillin" /gene="Ampicillin" CDS 1971..2831 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2986..3605 /label="pBR322_origin" misc_feature 3821..3847 /label="lacI.C" promoter 3914..3943 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC TCTAGAACTA 121 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG 181 GGGCGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 241 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 301 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 361 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 421 agCCAACACT TGTCACTACT CTCACTTATG GTGTTCAATG CTTCTCGAGA TACCCAGATC 481 ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 541 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 601 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 661 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 721 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG 781 GAATCAAAGT Tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 841 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 901 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA 961 CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 1021 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATgc ccgggggatc 1081 ggtggAGCTC CACCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT 1141 TCGATATCAA GCTTATCGAT ACCGTCGACC TCGAGGGGGG GCCCGGTACC CAATTCGCCC 1201 TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 1261 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 1321 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 1381 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 1441 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 1501 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 1561 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 1621 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 1681 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 1741 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 1801 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA 1861 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 1921 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 1981 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 2041 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 2101 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 2161 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG 2221 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 2281 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG 2341 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 2401 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 2461 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA 2521 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 2581 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC 2641 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 2701 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 2761 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA 2821 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC 2881 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 2941 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 3001 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 3061 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 3121 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 3181 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 3241 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 3301 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 3361 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 3421 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 3481 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 3541 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 3601 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG 3661 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC 3721 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA 3781 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT 3841 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 3901 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGA // 1542 LOCUS ADDGENE_1542 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3513 complete plasmid sequence KEYWORDS . SOURCE L3513 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1542 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3513" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1543 LOCUS ADDGENE_1543 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3514 complete plasmid sequence KEYWORDS . SOURCE L3514 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1543 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3514" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1544 LOCUS ADDGENE_1544 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3515 complete plasmid sequence KEYWORDS . SOURCE L3515 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1544 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3515" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1545 LOCUS ADDGENE_1545 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3516 complete plasmid sequence KEYWORDS . SOURCE L3516 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1545 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3516" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1546 LOCUS ADDGENE_1546 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3517 complete plasmid sequence KEYWORDS . SOURCE L3517 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1546 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3517" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2749..2768 /label="gfp.S65C" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 2761 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1547 LOCUS ADDGENE_1547 4688 bp DNA circular SYN 01/03/2005 DEFINITION L3522 complete plasmid sequence KEYWORDS . SOURCE L3522 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1547 at www.addgene.org FEATURES Location/Qualifiers source 1..4688 /organism="Addgene plasmid L3522" /mol_type="other DNA" misc_feature 50..72 /label="mec7.-836" misc_feature 862..885 /label="mec7.Tsa" misc_feature 957..998 /label="SynIVS.A" CDS 1029..1937 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 1029..1877 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1200..1250 /label="SynIVS.R" misc_feature 1251..1270 /label="gfp.F64L" misc_feature 1272..1291 /label="gfp.S65T" misc_feature 1405..1455 /label="SynIVS.S" misc_feature 1620..1670 /label="SynIVS.T" misc_feature 2043..2072 /label="let858.3'" promoter 2468..2496 /label="Amp_promoter" CDS 2538..3398 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2538..3398 /label="Ampicillin" /gene="Ampicillin" rep_origin 3553..4172 /label="pBR322_origin" misc_feature 4388..4414 /label="lacI.C" promoter 4481..4510 /label="lac_promoter" misc_feature 4545..4563 /label="M13r_primer" misc_feature 4577..4627 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGCCAA ATGTAAACCT 61 GTCATTTCTG tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagCAACAT CCGCGAGATC 901 GTGGATCCTA TCGATTCGCG GCCGCTGTAC ACCGGGATTG GCCAAAGGAC CCAAAGGTAT 961 GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG AGGGTACCGG 1021 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 1081 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 1141 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 1201 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 1261 TCACTACTCT CACTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC 1321 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 1381 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 1441 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 1501 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 1561 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 1621 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 1681 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1741 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1801 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1861 CTGGGATTAC ACATGGCATG GAcGAACTAT ACAAAagccc acaAGCTTTG GAATTCGCTA 1921 GCCGGCCATA CAAGTAATCC GGATGATCGA CGCCaACGTC GTTGAATTTT CAAATTTTAA 1981 ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC TCTTTGTGTT TTTTTTCTTG 2041 CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt ttttctcttt ccgtctccca 2101 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt 2161 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga 2221 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt 2281 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa 2341 attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCAAA AGGCCTTTTT TTTTTGGCGC 2401 CGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 2461 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 2521 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 2581 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 2641 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 2701 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 2761 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 2821 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 2881 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 2941 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 3001 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 3061 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 3121 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 3181 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 3241 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 3301 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 3361 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 3421 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 3481 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 3541 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 3601 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 3661 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 3721 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 3781 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 3841 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 3901 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 3961 ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 4021 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 4081 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 4141 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 4201 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA 4261 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 4321 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 4381 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 4441 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 4501 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC 4561 ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa 4621 ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA 4681 AATGAAAT // 1548 LOCUS ADDGENE_1548 4097 bp DNA circular SYN 01/03/2005 DEFINITION L3527 complete plasmid sequence KEYWORDS . SOURCE L3527 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1548 at www.addgene.org FEATURES Location/Qualifiers source 1..4097 /organism="Addgene plasmid L3527" /mol_type="other DNA" CDS 199..1071 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKR" gene 199..1059 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 370..420 /label="SynIVS.R" misc_feature 421..440 /label="gfp.F64L" misc_feature 442..461 /label="gfp.S65T" misc_feature 575..625 /label="SynIVS.S" misc_feature 790..840 /label="SynIVS.T" promoter complement(1252..1270) /label="T7_promoter" misc_feature 1290..1438 /label="lacZ_a" misc_feature complement(1281..1297) /label="M13f20_primer" rep_origin 1456..1762 /label="f1_origin" promoter 1955..1983 /label="Amp_promoter" gene 2025..2885 /label="Ampicillin" /gene="Ampicillin" CDS 2025..2885 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3040..3659 /label="pBR322_origin" misc_feature 3875..3901 /label="lacI.C" promoter 3968..3997 /label="lac_promoter" misc_feature 4032..4050 /label="M13r_primer" promoter 4067..4086 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgg tacgatatac taactaacaa gaattatcta atttttcagG 181 TCGGTACCGG TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT 241 CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA 301 GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT 361 GTTCCATGGg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag 421 CCAACACTTG TCACTACTCT CACTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT 481 ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT 541 ATATTTTTCA AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac 601 taaccataca tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT 661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA 721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA 781 ATCAAAGTTg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag 841 AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA 901 CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA 961 CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT 1021 GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAAAGgta agttttaaca 1081 tttaatcata cgaaatgaaa gagctccacc gcggtggcgg ccgctctaga actagtggat 1141 ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag gaattcgata 1201 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccCAATTC GCCCTATAGT 1261 GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 1321 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 1381 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC 1441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 1501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 1561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 1621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 1681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 1741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 1801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 1861 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC 1921 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 1981 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 2041 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2101 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2161 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 2221 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 2281 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 2341 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 2401 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 2461 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 2521 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 2581 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 2641 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2701 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2761 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2821 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 2881 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 2941 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3001 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3061 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3121 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3181 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3241 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3301 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3361 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3421 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3481 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3541 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3601 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3661 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 3721 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 3781 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 3841 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 3901 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 3961 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 4021 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT 4081 AAAGGGAACA AAAGCTG // 1549 LOCUS ADDGENE_1549 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3557 complete plasmid sequence KEYWORDS . SOURCE L3557 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1549 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3557" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1550 LOCUS ADDGENE_1550 6914 bp DNA circular SYN 05/14/2005 DEFINITION L3558 complete plasmid sequence KEYWORDS . SOURCE L3558 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1550 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3558" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1551 LOCUS ADDGENE_1551 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3559 complete plasmid sequence KEYWORDS . SOURCE L3559 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1551 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3559" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1552 LOCUS ADDGENE_1552 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3560 complete plasmid sequence KEYWORDS . SOURCE L3560 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1552 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3560" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1553 LOCUS ADDGENE_1553 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3561 complete plasmid sequence KEYWORDS . SOURCE L3561 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1553 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3561" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1554 LOCUS ADDGENE_1554 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3562 complete plasmid sequence KEYWORDS . SOURCE L3562 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1554 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3562" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1555 LOCUS ADDGENE_1555 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3563 complete plasmid sequence KEYWORDS . SOURCE L3563 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1555 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3563" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1556 LOCUS ADDGENE_1556 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3564 complete plasmid sequence KEYWORDS . SOURCE L3564 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1556 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3564" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1557 LOCUS ADDGENE_1557 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3565 complete plasmid sequence KEYWORDS . SOURCE L3565 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1557 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3565" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1558 LOCUS ADDGENE_1558 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3566 complete plasmid sequence KEYWORDS . SOURCE L3566 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1558 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3566" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1559 LOCUS ADDGENE_1559 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3567 complete plasmid sequence KEYWORDS . SOURCE L3567 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1559 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3567" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1560 LOCUS ADDGENE_1560 6859 bp DNA circular SYN 01/03/2005 DEFINITION L3568 complete plasmid sequence KEYWORDS . SOURCE L3568 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1560 at www.addgene.org FEATURES Location/Qualifiers source 1..6859 /organism="Addgene plasmid L3568" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3461..3511 /label="SynIVS.L" promoter 4639..4667 /label="Amp_promoter" gene 4709..5569 /label="Ampicillin" /gene="Ampicillin" CDS 4709..5569 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5724..6343 /label="pBR322_origin" misc_feature 6559..6585 /label="lacI.C" promoter 6652..6681 /label="lac_promoter" misc_feature 6716..6734 /label="M13r_primer" misc_feature 6748..6798 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC 3421 TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct 3481 actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA 3541 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 3601 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 3661 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 3721 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 3781 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 3841 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 3901 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 3961 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 4021 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 4081 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 4141 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 4201 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG 4261 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 4321 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG 4381 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA 4441 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4501 ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4561 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4621 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4681 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4741 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4801 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4861 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4921 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4981 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 5041 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 5101 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 5161 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 5221 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 5281 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 5341 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 5401 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5461 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5521 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5581 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5641 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5701 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5761 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5821 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5881 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5941 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 6001 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 6061 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 6121 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 6181 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 6241 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 6301 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 6361 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 6421 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 6481 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 6541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 6601 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 6661 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 6721 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 6781 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 6841 AACAACTTGG AAATGAAAT // 1561 LOCUS ADDGENE_1561 4755 bp DNA circular SYN 01/03/2005 DEFINITION L3569 complete plasmid sequence KEYWORDS . SOURCE L3569 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1561 at www.addgene.org FEATURES Location/Qualifiers source 1..4755 /organism="Addgene plasmid L3569" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 931..956 /label="gfp.Y145F" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1357..1407 /label="SynIVS.L" promoter 2535..2563 /label="Amp_promoter" gene 2605..3465 /label="Ampicillin" /gene="Ampicillin" CDS 2605..3465 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3620..4239 /label="pBR322_origin" misc_feature 4455..4481 /label="lacI.C" promoter 4548..4577 /label="lac_promoter" misc_feature 4612..4630 /label="M13r_primer" misc_feature 4644..4694 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTTCAA CTCACACAAC GTATACATCA 961 TGGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 1321 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta 1381 actaacgagt aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC 1441 AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT 1501 TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA 1561 CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT 1621 GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC 1681 ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA 1741 CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC 1801 GTCTGGGCCT CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG 1861 GAATTTTTCA ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT 1921 TTCCCCTATT GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT 1981 TGATGAGCAC GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT 2041 GAGTAGAAGT TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC 2101 AGAATACATT CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT 2161 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 2221 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 2281 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 2341 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 2401 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 2461 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 2521 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 2581 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 2641 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 2701 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 2761 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 2821 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 2881 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 2941 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 3001 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 3061 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 3121 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 3181 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 3241 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 3301 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 3361 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 3421 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 3481 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 3541 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 3601 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 3661 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 3721 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 3781 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 3841 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 3901 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 3961 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 4021 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 4081 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 4141 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 4201 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 4261 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 4321 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 4381 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 4441 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 4501 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 4561 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 4621 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 4681 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 4741 ACTTGGAAAT GAAAT // 1562 1563 LOCUS ADDGENE_1563 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3571 complete plasmid sequence KEYWORDS . SOURCE L3571 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1563 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3571" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 951..974 /label="gfp.M153A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCgAG ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCG 961 CAGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1564 LOCUS ADDGENE_1564 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3572 complete plasmid sequence KEYWORDS . SOURCE L3572 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1564 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3572" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2728..2747 /label="gfp.F64L" misc_feature 2749..2768 /label="gfp.S65T" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTCTCAC TTATGGTGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1565 LOCUS ADDGENE_1565 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3573 complete plasmid sequence KEYWORDS . SOURCE L3573 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1565 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3573" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1566 LOCUS ADDGENE_1566 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3574 complete plasmid sequence KEYWORDS . SOURCE L3574 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1566 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3574" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153T" misc_feature 3075..3096 /label="gfp.V163A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1567 LOCUS ADDGENE_1567 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3602 complete plasmid sequence KEYWORDS . SOURCE L3602 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1567 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3602" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1568 LOCUS ADDGENE_1568 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3603 complete plasmid sequence KEYWORDS . SOURCE L3603 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1568 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3603" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3036..3060 /label="gfp.N146I" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATATTTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1569 LOCUS ADDGENE_1569 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3604 complete plasmid sequence KEYWORDS . SOURCE L3604 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1569 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3604" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYIAADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 932..956 /label="gfp.N146I" misc_feature 951..974 /label="gfp.M153A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAT TTCACACAAC GTATACATCG 961 CAGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1570 LOCUS ADDGENE_1570 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3605 complete plasmid sequence KEYWORDS . SOURCE L3605 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1570 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3605" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" misc_feature 360..386 /label="SV40.NLS" CDS 360..1271 /label="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 932..956 /label="gfp.N146I" misc_feature 951..974 /label="gfp.M153T" misc_feature 971..992 /label="gfp.V163A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAT TTCACACAAC GTATACATCA 961 CTGCCGACAA ACAAAAGAAT GGGATCAAAG CGgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1571 LOCUS ADDGENE_1571 4755 bp DNA circular SYN 01/03/2005 DEFINITION L3606 complete plasmid sequence KEYWORDS . SOURCE L3606 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1571 at www.addgene.org FEATURES Location/Qualifiers source 1..4755 /organism="Addgene plasmid L3606" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 932..956 /label="gfp.N146I" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1357..1407 /label="SynIVS.L" promoter 2535..2563 /label="Amp_promoter" gene 2605..3465 /label="Ampicillin" /gene="Ampicillin" CDS 2605..3465 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3620..4239 /label="pBR322_origin" misc_feature 4455..4481 /label="lacI.C" promoter 4548..4577 /label="lac_promoter" misc_feature 4612..4630 /label="M13r_primer" misc_feature 4644..4694 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAT TTCACACAAC GTATACATCA 961 TGGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 1321 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta 1381 actaacgagt aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC 1441 AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT 1501 TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA 1561 CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT 1621 GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC 1681 ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA 1741 CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC 1801 GTCTGGGCCT CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG 1861 GAATTTTTCA ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT 1921 TTCCCCTATT GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT 1981 TGATGAGCAC GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT 2041 GAGTAGAAGT TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC 2101 AGAATACATT CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT 2161 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 2221 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 2281 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 2341 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 2401 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 2461 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 2521 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 2581 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 2641 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 2701 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 2761 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 2821 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 2881 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 2941 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 3001 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 3061 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 3121 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 3181 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 3241 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 3301 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 3361 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 3421 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 3481 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 3541 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 3601 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 3661 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 3721 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 3781 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 3841 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 3901 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 3961 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 4021 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 4081 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 4141 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 4201 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 4261 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 4321 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 4381 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 4441 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 4501 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 4561 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 4621 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 4681 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 4741 ACTTGGAAAT GAAAT // 1572 LOCUS ADDGENE_1572 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3607 complete plasmid sequence KEYWORDS . SOURCE L3607 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1572 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3607" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1573 LOCUS ADDGENE_1573 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3608 complete plasmid sequence KEYWORDS . SOURCE L3608 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1573 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3608" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3035..3060 /label="gfp.Y145F" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT TCAACTCACA CAACGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1574 LOCUS ADDGENE_1574 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3609 complete plasmid sequence KEYWORDS . SOURCE L3609 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1574 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3609" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIAADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 931..956 /label="gfp.Y145F" misc_feature 951..974 /label="gfp.M153A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTTCAA CTCACACAAC GTATACATCG 961 CAGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1575 LOCUS ADDGENE_1575 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3610 complete plasmid sequence KEYWORDS . SOURCE L3610 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1575 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3610" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" misc_feature 360..386 /label="SV40.NLS" CDS 360..1271 /label="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 931..956 /label="gfp.Y145F" misc_feature 951..974 /label="gfp.M153T" misc_feature 971..992 /label="gfp.V163A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCGAG ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTTCAA CTCACACAAC GTATACATCA 961 CTGCCGACAA ACAAAAGAAT GGGATCAAAG CGgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1576 LOCUS ADDGENE_1576 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3611 complete plasmid sequence KEYWORDS . SOURCE L3611 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1576 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3611" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66H" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ACACGGCGTA 2761 CAATGCTTCT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1577 LOCUS ADDGENE_1577 6914 bp DNA circular SYN 01/03/2005 DEFINITION L3612 complete plasmid sequence KEYWORDS . SOURCE L3612 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1577 at www.addgene.org FEATURES Location/Qualifiers source 1..6914 /organism="Addgene plasmid L3612" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2506..3375 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2506..3366 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2677..2727 /label="SynIVS.R" misc_feature 2752..2776 /label="gfp.Y66W" misc_feature 2882..2932 /label="SynIVS.S" misc_feature 3055..3078 /label="gfp.M153A" misc_feature 3097..3147 /label="SynIVS.T" misc_feature 3389..3439 /label="SynIVS.M" misc_feature 3516..3566 /label="SynIVS.L" promoter 4694..4722 /label="Amp_promoter" gene 4764..5624 /label="Ampicillin" /gene="Ampicillin" CDS 4764..5624 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5779..6398 /label="pBR322_origin" misc_feature 6614..6640 /label="lacI.C" promoter 6707..6736 /label="lac_promoter" misc_feature 6771..6789 /label="M13r_primer" misc_feature 6803..6853 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 2521 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 2581 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 2641 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 2701 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTC ATGGGGCGTT 2761 CAATGCTTCT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 2821 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2881 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2941 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 3001 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 3061 ATCGCAGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 3121 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 3181 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 3241 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 3301 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 3361 GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac tttctcatac taactaacgc 3421 gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3481 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 3541 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 3601 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 3661 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 3721 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 3781 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 3841 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 3901 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 3961 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 4021 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 4081 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 4141 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 4201 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 4261 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 4321 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4381 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4441 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4501 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4561 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4621 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4681 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4741 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4801 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4861 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4921 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4981 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5041 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5101 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5161 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5221 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5281 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5341 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5401 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5461 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5521 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5581 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5641 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5701 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5761 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5821 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5881 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5941 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6001 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6061 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6121 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6181 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6241 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6301 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6361 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6421 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6481 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6541 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6601 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6661 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6721 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6781 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6841 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6901 CTTGGAAATG AAAT // 1578 LOCUS ADDGENE_1578 4810 bp DNA circular SYN 01/03/2005 DEFINITION L3613 complete plasmid sequence KEYWORDS . SOURCE L3613 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1578 at www.addgene.org FEATURES Location/Qualifiers source 1..4810 /organism="Addgene plasmid L3613" /mol_type="other DNA" misc_feature 29..54 /label="unc54.-204" misc_feature 244..267 /label="unc54.5'" misc_feature 285..326 /label="SynIVS.A" CDS 360..1271 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 360..386 /label="SV40.NLS" gene 402..1262 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 573..623 /label="SynIVS.R" misc_feature 645..664 /label="gfp.S65T" misc_feature 778..828 /label="SynIVS.S" misc_feature 951..974 /label="gfp.M153T" misc_feature 971..992 /label="gfp.V163A" misc_feature 993..1043 /label="SynIVS.T" misc_feature 1285..1335 /label="SynIVS.M" misc_feature 1412..1462 /label="SynIVS.L" promoter 2590..2618 /label="Amp_promoter" gene 2660..3520 /label="Ampicillin" /gene="Ampicillin" CDS 2660..3520 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3675..4294 /label="pBR322_origin" misc_feature 4510..4536 /label="lacI.C" promoter 4603..4632 /label="lac_promoter" misc_feature 4667..4685 /label="M13r_primer" misc_feature 4699..4749 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAA TTCTTGAGAC TTCCACTATC 61 TTCTCTGGCA CCCTCCACTA CGCGTAGGCG TCTCTCGCTT CGTGTATTCC CGGGAAGCCG 121 GGTTCCCGTC TCTCCCGCCG CTGCCGCTGC CGCACACAGC TTTACACCTC GTAGAATCCC 181 CAAAGAGGGG CGTGGCTTGC GGGTGCCAAC ATCCTCCTGC CGAGGAAGAA GCAGGCACTC 241 ATCACTCGCA TCATCAACCT CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT 301 ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG GGTACCGAGC TCAGAAAAAA 361 TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC 421 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT 481 TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA 541 TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta 601 accctgatta tttaaatttt cagCCAACAC TTGTCACTAC TTTCaCTTAT GGTGTTCAAT 661 GCTTcTCgAG ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT GCCATGCCCG 721 AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta 781 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA 841 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG 901 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA 961 CTGCCGACAA ACAAAAGAAT GGGATCAAAG CGgtaagttt aaacatgatt ttactaacta 1021 actaatctga tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG 1081 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC 1141 CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG 1201 ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC 1261 TATACAAATA GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc 1321 tatttaaatt ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 1381 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 1441 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 1501 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 1561 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 1621 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 1681 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 1741 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 1801 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 1861 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 1921 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 1981 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 2041 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 2101 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 2161 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 2221 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2281 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2341 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2401 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2461 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2521 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2581 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2641 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2701 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2761 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2821 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2881 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2941 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3001 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3061 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3121 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3181 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3241 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3301 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3361 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3421 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3481 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3541 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3601 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3661 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3721 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3781 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3841 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3901 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3961 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4021 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4081 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4141 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4201 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4261 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4321 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4381 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4441 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4501 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4561 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4621 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4681 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4741 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4801 GAAATGAAAT // 1579 LOCUS ADDGENE_1579 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3615 complete plasmid sequence KEYWORDS . SOURCE L3615 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1579 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3615" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1972 /label="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 590..640 /label="SynIVS.R" misc_feature 641..660 /label="gfp.F64L" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 949..973 /label="gfp.N146I" misc_feature 968..991 /label="gfp.M153T" misc_feature 988..1009 /label="gfp.V163A" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTCT 661 CACTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATATTTC 961 ACACAACGTA TACATCACTG CCGACAAACA AAAGAATGGG ATCAAAGCGg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1580 LOCUS ADDGENE_1580 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3616 complete plasmid sequence KEYWORDS . SOURCE L3616 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1580 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3616" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1972 /label="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" misc_feature 590..640 /label="SynIVS.R" misc_feature 665..689 /label="gfp.Y66W" misc_feature 795..845 /label="SynIVS.S" misc_feature 949..973 /label="gfp.N146I" misc_feature 968..991 /label="gfp.M153T" misc_feature 988..1009 /label="gfp.V163A" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTCATGGGGC GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATATTTC 961 ACACAACGTA TACATCACTG CCGACAAACA AAAGAATGGG ATCAAAGCGg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1581 LOCUS ADDGENE_1581 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3617 complete plasmid sequence KEYWORDS . SOURCE L3617 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1581 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3617" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1972 /label="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 590..640 /label="SynIVS.R" misc_feature 641..660 /label="gfp.F64L" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 948..973 /label="gfp.Y145F" misc_feature 968..991 /label="gfp.M153T" misc_feature 988..1009 /label="gfp.V163A" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTCT 661 CACTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTTCAACTC 961 ACACAACGTA TACATCACTG CCGACAAACA AAAGAATGGG ATCAAAGCGg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1582 LOCUS ADDGENE_1582 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3618 complete plasmid sequence KEYWORDS . SOURCE L3618 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1582 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3618" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSHGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 665..689 /label="gfp.Y66H" misc_feature 795..845 /label="SynIVS.S" misc_feature 948..973 /label="gfp.Y145F" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTCACACGGC GTACAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTTCAACTC 961 ACACAACGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1583 LOCUS ADDGENE_1583 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3619 complete plasmid sequence KEYWORDS . SOURCE L3619 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1583 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3619" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 665..689 /label="gfp.Y66W" misc_feature 795..845 /label="SynIVS.S" misc_feature 948..973 /label="gfp.Y145F" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTCATGGGGC GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTTCAACTC 961 ACACAACGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1584 LOCUS ADDGENE_1584 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3620 complete plasmid sequence KEYWORDS . SOURCE L3620 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1584 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3620" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 641..660 /label="gfp.F64L" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 968..991 /label="gfp.M153A" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTCT 661 CACTTATGGT GTTCAATGCT TCTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCGCAG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1585 LOCUS ADDGENE_1585 8217 bp DNA circular SYN 01/03/2005 DEFINITION L3621 complete plasmid sequence KEYWORDS . SOURCE L3621 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1585 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid L3621" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 968..991 /label="gfp.M153A" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CaCTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCGCAG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1586 LOCUS ADDGENE_1586 11700 bp DNA circular SYN 01/03/2005 DEFINITION L3670 complete plasmid sequence KEYWORDS . SOURCE L3670 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1586 at www.addgene.org FEATURES Location/Qualifiers source 1..11700 /organism="Addgene plasmid L3670" /mol_type="other DNA" misc_feature 4648..4671 /label="let858.Tsa" misc_feature 4699..4772 /label="ATG-MitLS" CDS 4699..6015 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MALLQSRLLLSAPRRAAATARASSVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPVRSRSPRRSSARSP SRSPRRRRERSSERKQSEEPAPLPEKKKKEPLDILRTRTGGAYIPPAKLRLMQQQISD KQSEQYQRMNWERMKKKIHGLVNRVNAKNLVQIVRELLQENVIRSK" gene 4786..5646 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 4957..5007 /label="SynIVS.R" misc_feature 5029..5048 /label="gfp.S65C" misc_feature 5162..5212 /label="SynIVS.S" misc_feature 5377..5427 /label="SynIVS.T" CDS 7733..8437 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 8413..8437 /label="let858.C" misc_feature 8515..8544 /label="let858.3'" promoter 9614..9642 /label="Amp_promoter" CDS 9684..10544 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 9684..10544 /label="Ampicillin" /gene="Ampicillin" rep_origin 10699..11318 /label="pBR322_origin" misc_feature 11534..11560 /label="lacI.C" promoter 11627..11656 /label="lac_promoter" misc_feature 11691..11709 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGga tcttctggct 61 tctgtgcagc tgcatcgctt tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa 121 ccttgttgag ttcatcaact gaatctgtga ctggcttgtt gctcactgga tgcactagac 181 gactgattct cgagaaatca gattgagttg cgattagggt gacctagaaa ttgggaataa 241 tacgaacttt tgaaaatatt caggaggatt aaaaaaatta ttctcgacaa tcctacaaat 301 ttacttattg caccatgttg ctccaacatt tttcattaaa agttaatgaa aaaatgtaga 361 aaatcggaaa ttggcaattt tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct 421 gaaataaatg tcattttcag ataaatcgag cgattttctg ttgtctgaca ctagttttta 481 gttttaaaaa atgttggaag aacatggtgc aataggtaat ttcatagaat ttccatgtgt 541 tttttttcaa ttaaccaatt atccaaatct tccaaactca cattttgcgg agctgggcta 601 tcaagaatct gctgcagttt tataagacga gcatctctga tatcactgaa aattaatttt 661 taatcaaaac ttgaatatca actaaaccca cttattaact ttctcgatct tctgtcgttc 721 ggtacgatga cggtgaagaa gccaattgta gtagttgatt tggttcaagt cctttcggtg 781 ttgtacgtca gtgtcctgca atgctattta gttataactt aggcctaaga ttcaatttaa 841 tgaagtgatt aaatttgttc tctgaacctc ttaagatgat cttttggatt agaaacatat 901 aagacaggtt tacctatcta ttaaaaaaca gatcaaaata gatacgacca aatcggataa 961 tccatgccta cctggcatct aggaacgtgt tcttagaaga tttcttacgt aatcgtatga 1021 agaaataaca atttgatcgt tggccagcaa aaatagggtt ttaagtggga tagtgttttt 1081 attagctaac cggaaaattt tatagttttt ttttgcaaga aaccactgaa aaccccctaa 1141 ttgtatacat tttttggagc agcttctggt ctttttgagc aataaaattc gataaaacag 1201 aatttaagtg taaattgttc acatttagtt tctattttat caaattttgt tgctcaaaaa 1261 cattcgaagc tgctctaaaa aaatgcatta aaaaaggggt tttcagtggt ttttcacatt 1321 aaaaaagcta attttaacta aaaatccatc atatttccaa ctttgtcaca acaataaaat 1381 gctggtcaaa atgtgttcga aaaaatgttt ttttttttaa tttttataat ttaaaaatag 1441 ttttctttcg ctgggacaca tacatttttg ggcgtaaatt ttcagttcaa atttccattt 1501 ttacaaccat aatcataaag ctacgtctga tctctctcgc acttacctgc gcctgattcg 1561 aaagaacaac cgtagccaaa agaacaagaa gaacaagcac gtagttgtgg tagtggacgt 1621 tcatcacgca atactgacca atggtcgtgg ggtctcactt tccgtactat tgagagaggg 1681 gagactgaag atggcaattg aggacagtgt cttcgacgca cgCATGCCTG CAGGtcgacg 1741 gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagacaag 1801 ctttatcatc ttgtcgcacg ttatcttcaa ttgaaactac acagatgatg aaacggacat 1861 aaagggttag tttacaaatg ttccggttct tttttacaat tgaatgcaaa ggtatcattg 1921 aaacaattgg gtgataacga tggtatttta gattttgaga tttgaatgaa acagtgtaga 1981 atgtgatatt gaacaaaggg aattcattta tgactttcta aaacgtatcg cccttttcat 2041 ttcaatttct gattctgtgg aaattttgtg gttctcaacg ttaacaaaac aatcgtttca 2101 ggccaaaatt ttcccagttt ttgtaaaatt tgcctacttt tattatattc gaaaataatg 2161 aaaatatgat ttttaaatcg aacgatattt aagctttcca ggcaaaatga aaattttcag 2221 atcaattttt tactaacttt gacggcctga gaattgttat ttttggagaa ttttgagaat 2281 ttgaaaagtg ataagtatac ttagtttcaa aaaaaaaggg aagttctttc taaatatctg 2341 aaatttcttt atttaattta taaaaggttg ttttcctaca cctattaacc ccagcaaaca 2401 taattaaaaa taaccatttt agtgcaattt tacttttaaa ttatagacat aaaatatttt 2461 aaggatattt cacaattttt tttttcaatt ttgaaatgct ttcagatggc taaaaccttg 2521 tttgaagcaa tttttcaaac atcgaaatca tggacttgaa caatttcaaa ttcaattttt 2581 caggagctgg acattgccgc tggaattttt aaataactca actaaatttg acagatattt 2641 tcagataaaa ttactagctt caaagcctga tccctttatt aattcaaata aaagttacaa 2701 cgtttaaaac caacgagatg ggcttttttt aattcaagct tcggccctat aaatcaacaa 2761 gacccataaa ttgtgatgta taaatcttca atcttaaatc tcataatcat tgatatgatc 2821 aatcaaaact ttaatatata attaaagaaa cacctctatg gattacatca atgtttatcc 2881 aacaaattgc ctaatccaat tcctctctga acacctggtt tagcatctct ccactgccga 2941 aaatgcatgg aaacaagcgt catatcagat atccgatagg ttgcttcatt tcagttttct 3001 tctctctttg cttcgtgttt cttataaaca aaaagaaaca caaatctttg atgaatgaaa 3061 attcagacat cgttggaggg gatggttgac cttcaggcgg ctcaattcat taccagtttc 3121 acaaaaaatt cgatgattat ctctataaat tgtcaagaga aaatgaaaaa aagaaaacct 3181 tttcgctttc tgccgagcca tgacaagatg cctatctttt actattttct ctctgtcact 3241 ttcggtaaac ccgccaagga cggacacgat ttcatgcttc ggttgtaaac aaattcagtg 3301 tgaataagaa gaatattcgg aggaaaggcg acggaaatta tcaaatttac agccagacat 3361 catgtgagac aagagaacta tatgaatatg caaaataaat tgaattggat tgtttagttg 3421 gccagttgga agggagtcat agaatgaaca cacaaattca gttatcaaaa aagaagtcta 3481 gcttgagttc ttcacgaatt atgatcctag aactattcag gaattatgat cctagaacta 3541 ttcaggaatt atttcagaaa tcggaaaact aaataaccgg taaaatgttc acaaaaccct 3601 tttggaatta taataagttt tccaaaaaaa gattgataat tttacattca aaatcacaca 3661 ctttcgcttt tgaggcaggt tttgagcagc ttttcaaaac tttaaattgg aagtgaagtt 3721 agtttcaaga agtaaaaata tttctctata cttttatcta aatttccaat caaaaaactt 3781 tgaaaaacga tgaaaaaatg gagattttgt aagcgagccg aaaaaaagtt cagcacatat 3841 ctgttacctg ccatatactg aacatgacaa aatatatcag aacaaaatta agttttatgc 3901 atagtaggga tgtttttgca tattttatca ctgaaaaatc ttttaatatc agtagtttca 3961 gaaaagttac atgccatttt ctgaaaattt caatttttgc tcataaaatc ctccattttc 4021 aaaattttcg cttcaaattt tgattaaaca tgttattttt gagtcttcta ttagattctg 4081 aaagaatttt ttaaaaatgt tggctcatct ccaaaatcgt cttccgctct gaaaaacgaa 4141 agtggacctt tgacatccga aaaaatgggc gaaaaaatga aattgagctt tttgggtcga 4201 aaaaaatgtt tttagaatgc tgagaacacg ttaaacacga agatcatatt tattttgaga 4261 cccggatgct ctgaaaatgt ctgacataga tttaaaaaag catatatata tttttcattt 4321 tcaacgtgaa agttttgtgc aactttatag aatctcctat tggcacattg ttttttattt 4381 aactgaggca gtttttgaac acctttttga aactttgaat ctctttgaag tatactgtcg 4441 aaaagactga cttgagcgtt cgaaatgcca gaagaaaact atatttgaat ctcgcgctaa 4501 attgagaaat gcaaccgcgc tccactggac aattggaaaa aaaatttatt cggaggcgac 4561 aacggtattt tcgaaattga ttttctgtgt attttctcat tttttataaa ttcttctttg 4621 atttatcgtt cgtttgtgag aaatttaatt gtattcaaac ttttttatag TAAGATaccg 4681 gtgGTACctc gagaaaaaat ggcactcctg caatcacgtc tcctgctatc cgcacctcgt 4741 cgtgccgctg caactgcaag agcttcatcc GTACCGGTAG AAAAAATGAG TAAAGGAGAA 4801 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 4861 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 4921 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 4981 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 5041 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 5101 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 5161 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 5221 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 5281 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 5341 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 5401 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 5461 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 5521 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 5581 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT 5641 GAACTATACA AATgcCCGGT TCGTTCAAGA TCTCCCAGAA GGTCTTCAGC ACGTTCCCCG 5701 TCACGATCTC CTAGACGGCG CCGAGAAAGA AGCTCAGAAA GAAAGCAATC CGAAGAGCCA 5761 GCACCGCTAC CAGAGAAAAA GAAGAAAGAG CCGCTGGATA TTCTACGAAC AAGAACCGGA 5821 GGAGCATATA TTCCACCCGC CAAACTTCGA CTTATGCAAC AACAGATTAG TGATAAGCAA 5881 AGTGAACAGT ATCAGAGAAT GAATTGGGAA AGAATGAAGA AAAAGATTCA CGGATTGGTT 5941 AACAGAGTCA ACGCGAAGAA TCTTGTTCAA ATTGTCAGAG AACTTCTTCA AGAGAATGTG 6001 ATTCGTTCAA Agtgagtgag aaaatcgaag gaaaaggaaa gaattaattt aatttttcag 6061 GGGACTTCTC TGCCGTGACA TTATTCAAGC TCAGGCTTTC TCACCAGGAT TCTCTAACGT 6121 CTATGCAGCT TTGGCGGCAG TTATCAACTC GAAATTCCCT CATGTCGGTG AACTTCTTCT 6181 CCGTCGTCTG ATTGTACAGT TCAAAAGAAG TTTCCGTAGA AATGACAGAG GCGTCACGGT 6241 GAACGTGATC AAATTCATCG CACATTTGAT TAATCAACAA GTTGCTCACG AAGTTCTTGC 6301 GCTGGAAATC ATGATTCTGA TGCTTGAAGA ACCAACTGAT GATTCAGTTG AAGTCGCCAT 6361 TGCGTTCCTG AAAGAGTGTG GAGCAAAGCT TCTGGAGATT GCTCCAGCAG CTCTTAACAG 6421 TGTCTACGAC CGTCTTCGTG CAATTCTCAT GGAAACTGAA AGATCGGAAA ATGCACTGGA 6481 TCGACGTATT CAGTATATGA TTGAGACTGC AATGCAGATT CGAAAGGACA AATTTGCGGC 6541 GTATCCGGCG GTCATTGAAG ACTTGGACTT GATTGAGGAG GAGGATCAGA TCATCCATAC 6601 ACTTAATTTG GAGGATGCGG TTGATCCGGA AAATGGGCTT AATGTGTTCA AACTAGATCC 6661 AGAATTCGAA AAGAACGAGG AGGTTTATGA GGAGATCCGT AAGGAAATCA TTGGAAACGC 6721 CGATATTTCG GATGAGGATG GTGGCGACGA GTTGGATGAT GAAGAAGAGG GTAGTGATGT 6781 GGAAGAGGCT CCGAAGAAGA CTACAGAGAT TATTGATAAT ACTGATCAGA ATTGACTGCT 6841 TTCAGAAGgt attcattttg agttttgggc cggcaaatct gtaagttgcc ggttgccgaa 6901 aatttgctga atttgccgga aaaaaaaatt ccggaattta tttaaaaact ttttgtaaaa 6961 attaaattaa atttgcaact tttcagAGAA GTCTACCTGA CAATGCAATC ATCTTTGGAC 7021 TACCAAGAAG CTGCTCACAA ATTGCTGAAA ATGAAGATTC CAGACAGCAT GCAGgtcagc 7081 gatgttgcaa agaaaaattt tcgaccaaaa aaaccaacca atcataaaat ttaaaaaaaa 7141 actccgtttt tttctttttt tttatacgag aaaaaccaaa aaaatgtatt tttgccaaat 7201 tctaaaatac tatccccgaa attttcaata ttttctcttt cagAACGAAC TCTGCGCGAT 7261 GCTTGTCGAT TGTTGTGCTC AACAGCGTAC CTACGAGCGA TTCTACGGAA TGCTCATCGA 7321 ACGTTTCTGC CGACTTCGCC TCGAATACCA GCAATACTTT GAAAAGCTCT GCCAGGACAC 7381 GTATTCCACG ATTCACCGAA TTGACATCAC AAAACTGCGG AATTTGGCTC GCCTTATTGC 7441 TCATTTGCTC TCGACGGATG CTATTGACTG GAAGATTTTG GCCGATATGA AAATGACCGA 7501 AGAGGACACA ACTTCTTCTG GCAGAATCTA TATTAAATAT ATATTTAATG AACTTGTGGA 7561 GGCGATGGGA ATGGTTAAAC TTCATTCGAG AGTTACTGAT CCgtgagttt cctagagaga 7621 gttgttttcg tattcaattt tccctatttt cagAACTTTG GCTCATTGCT TTGTTGGATT 7681 ATTCCCACGA ACTAATCCGA ACAGCGCACG ATTTTCGATC AACTTCTTCA CAATGATTGG 7741 ATTGGGTGGT TTGACGTTGG AACTTCGTGA ATGGCTGGCA AAGGGTCTCA AGAAGAAGAA 7801 GGGAATGCTG GATCAGTTGA AGGCCGAATC AAGCTCAGAT TCATCGTCGT CTTCGGATTC 7861 GTCAGACTCG TCTGATTCTT CGGATTCTGA CGATTCATCC GACTCGTCTT CAGATTCCTC 7921 ATCTTCTTCA GAATCAGAGC CAGAACCACC GAAGAAAAAG AAGAAGAAGA ACAGTGAAGA 7981 GAGTTCCAAA AAGAAGGAAA AAGAGAATAT TGGTCGACGG GATCGTGGAG ACAAGAGAGC 8041 TGAACGTCAT CGTGATCAAA GTGTGGAGAA CAAGGACAAG GATCGTCGAC GTCGCCAGGA 8101 TTCTGACGAA AATCGTCGGC CAGAACGAGG AGATGACCGC AAGGATCGGA GTAAAGATCG 8161 TCGTCGTCAA GACTCGGATG ATGAGGATCG GAAAGGTCGT GAACGTCGGG AAGATTCAGG 8221 GGAAAGACGT CGCGGAGATC GGGATCGACG TGATCGAAAC AAGGATCAGG AGGATCACCG 8281 TGAAGATCGC CGTGACCGAA GCAAGGATCG TGAGGATCGA CGTGATCGCC GTCGTCATGA 8341 CTCTGATGAT GATCGTAAAA CTCGTCGGGA TAGAAGTGAA GAGCGAGGAG GACGTCGTCG 8401 TGAAGTGGAA TCGGATGATC GACGCCGACG TCGTTGAATT TTCAAATTTT AAATACTGAA 8461 TATTTGTTTT TTTTCCTATT ATTTATTTAT TCTCTTTGTG TTTTTTTTCT TGCTTTCTAA 8521 AAAATTAATT CAATCCAAAT CTAAacattt ttttttctct ttccgtctcc caattcgtat 8581 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat 8641 taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat 8701 aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa 8761 acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg 8821 aacgatgaga attgtcctcg cttggcaaat gcgaatccgt atggagaggc acgtttggcg 8881 aaggcaaatg ttcggtatgg agatctgtaa aaatttttaa gttgaaattt ggtgttgctc 8941 ttttacaaaa ttttccgatt ttcgcttgaa attacggtgc caggtctcga cacgtcttcc 9001 aatttttcaa attcaaaaga gcctttaatg ggctgtagtt gctaatttct cgtttttgaa 9061 aatttttctt ccgtttaatc gaaatttgat gtattttatt tatgattttc aataaatttc 9121 aaagaaactg gtgaaaactc ggaaaattgt gaactacagt aatccaatcc ttaaaggcgc 9181 acacctttta aatgtccgcc ccaatacgat atttttttaa gattcgCTAG AGCGGCCCTT 9241 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 9301 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 9361 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 9421 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 9481 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 9541 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 9601 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 9661 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 9721 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 9781 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 9841 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 9901 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 9961 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 10021 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 10081 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 10141 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 10201 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 10261 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 10321 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 10381 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 10441 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 10501 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 10561 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 10621 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 10681 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 10741 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 10801 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 10861 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 10921 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 10981 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 11041 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 11101 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 11161 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 11221 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 11281 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 11341 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 11401 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 11461 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 11521 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 11581 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 11641 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1587 LOCUS ADDGENE_1587 4634 bp DNA circular SYN 01/03/2005 DEFINITION L3691 complete plasmid sequence KEYWORDS . SOURCE L3691 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1587 at www.addgene.org FEATURES Location/Qualifiers source 1..4634 /organism="Addgene plasmid L3691" /mol_type="other DNA" misc_feature 50..72 /label="mec7.-836" misc_feature 862..885 /label="mec7.Tsa" promoter complement(940..958) /label="T7_promoter" CDS 975..1883 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 975..1823 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1146..1196 /label="SynIVS.R" misc_feature 1218..1237 /label="gfp.S65C" misc_feature 1351..1401 /label="SynIVS.S" misc_feature 1566..1616 /label="SynIVS.T" misc_feature 1989..2018 /label="let858.3'" promoter 2414..2442 /label="Amp_promoter" gene 2484..3344 /label="Ampicillin" /gene="Ampicillin" CDS 2484..3344 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3499..4118 /label="pBR322_origin" misc_feature 4334..4360 /label="lacI.C" promoter 4427..4456 /label="lac_promoter" misc_feature 4491..4509 /label="M13r_primer" misc_feature 4523..4573 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGCCAA ATGTAAACCT 61 GTCATTTCTG tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagCAACAT CCGCGAGATC 901 GTGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 961 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 1021 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 1081 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 1141 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 1201 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA 1261 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 1321 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 1381 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 1441 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 1501 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 1561 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 1621 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 1681 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 1741 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1801 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 1861 TCGCTAGCCG GCCATACAAG TAATCCGGAT GATCGACGCC aACGTCGTTG AATTTTCAAA 1921 TTTTAAATAC TGAATATTTG TTTTTTTTCC TATTATTTAT TTATTCTCTT TGTGTTTTTT 1981 TTCTTGCTTT CTAAAAAATT AATTCAATCC AAATCTAAac attttttttt ctctttccgt 2041 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct 2101 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat 2161 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga 2221 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc 2281 aaagaaatta ggcgaacgat gAGAATTGTC CTCGCTTGGG CCCAAAAGGC CTTTTTTTTT 2341 TGGCGCCGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 2401 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 2461 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 2521 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 2581 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 2641 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 2701 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 2761 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 2821 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 2881 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 2941 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 3001 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 3061 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 3121 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 3181 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 3241 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 3301 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 3361 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 3421 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 3481 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 3541 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 3601 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 3661 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 3721 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 3781 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 3841 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 3901 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 3961 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 4021 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 4081 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 4141 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 4201 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 4261 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 4321 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 4381 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 4441 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 4501 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 4561 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 4621 CTTGGAAATG AAAT // 1588 LOCUS ADDGENE_1588 10449 bp DNA circular SYN 01/03/2005 DEFINITION L3720 complete plasmid sequence KEYWORDS . SOURCE L3720 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1588 at www.addgene.org FEATURES Location/Qualifiers source 1..10449 /organism="Addgene plasmid L3720" /mol_type="other DNA" misc_feature 50..72 /label="mec7.-836" misc_feature 862..885 /label="mec7.Tsa" promoter complement(940..958) /label="T7_promoter" CDS 975..1883 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 1146..1196 /label="SynIVS.R" misc_feature 1197..1216 /label="gfp.F64L" misc_feature 1218..1237 /label="gfp.S65T" misc_feature 1351..1401 /label="SynIVS.S" misc_feature 1524..1547 /label="gfp.M153A" misc_feature 1566..1616 /label="SynIVS.T" misc_feature 1881..1934 /label="let858.smgUTR" CDS 6633..7337 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 7313..7337 /label="let858.C" misc_feature 7415..7444 /label="let858.3'" promoter 8229..8257 /label="Amp_promoter" CDS 8299..9159 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8299..9159 /label="Ampicillin" /gene="Ampicillin" rep_origin 9314..9933 /label="pBR322_origin" misc_feature 10149..10175 /label="lacI.C" promoter 10242..10271 /label="lac_promoter" misc_feature 10306..10324 /label="M13r_primer" misc_feature 10338..10388 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGCCAA ATGTAAACCT 61 GTCATTTCTG tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagCAACAT CCGCGAGATC 901 GTGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 961 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 1021 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 1081 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 1141 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 1201 CACTTGTCAC TACTCTCACT TATGGTGTTC AATGCTTCTC gAGATACCCA GATCATATGA 1261 AACgGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 1321 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 1381 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 1441 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 1501 AATACAACTA TAACTCACAC AATGTATACA TCGCAGCAGA CAAACAAAAG AATGGAATCA 1561 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 1621 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 1681 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 1741 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1801 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 1861 TCGCTAGCCG GCCATACAAG TAATccgggt tctagaagcg ctgctaaggg ggcccTCGTC 1921 GAGTCGGTCA CAATCACCTG AAACTCCAAA GGCAGCCAGT GAGGAACGTG AAGAAGAAGA 1981 AAAAGAGTCA TCTGAACAGg tttgattttc tttctggtca aaaagatgaa attattgatt 2041 ttcagCCAGA TACTCCCAAA ACTAGCAGCG AGAAGTCTGC AAGTCGTTCA CAGTCGCCCA 2101 GAGAATCGCG GGAAGTGAGC CAAGAGgtat gtttttcaaa aatcaataac tgatcataat 2161 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat 2221 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat 2281 ttcagACTGA AACTTCGGAA AATCAAGAGA AAATCAAAGA AAAGGATGAC GGGGATGATC 2341 AGCCTGGCAC ACCGAACAGC TATAGAAGCC GGGAAACTTC ACCAGCTCCA AAAAGGTCCA 2401 AGGAGACCAG gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagAGAA 2461 TCAGAGTCTC CTGAAAAATC CCCGGTTCGT TCAAGATCTC CCAGAAGGTC TTCAGCACGT 2521 TCCCCGTCAC GATCTCCTAG ACGGCGCCGA GAAAGAAGCT CAGAAAGAAA GCAATCCGAA 2581 GAGCCAGCAC CGCTACCAGA GAAAAAGAAG AAAGAGCCGC TGGATATTCT ACGAACAAGA 2641 ACCGGAGGAG CATATATTCC ACCCGCCAAA CTTCGACTTA TGCAACAACA GATTAGTGAT 2701 AAGCAAAGTG AACAGTATCA GAGAATGAAT TGGGAAAGAA TGAAGAAAAA GATTCACGGA 2761 TTGGTTAACA GAGTCAACGC GAAGAATCTT GTTCAAATTG TCAGAGAACT TCTTCAAGAG 2821 AATGTGATTC GTTCAAAgtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt 2881 tttcagGGGA CTTCTCTGCC GTGACATTAT TCAAGCTCAG GCTTTCTCAC CAGGATTCTC 2941 TAACGTCTAT GCAGCTTTGG CGGCAGTTAT CAACTCGAAA TTCCCTCATG TCGGTGAACT 3001 TCTTCTCCGT CGTCTGATTG TACAGTTCAA AAGAAGTTTC CGTAGAAATG ACAGAGGCGT 3061 CACGGTGAAC GTGATCAAAT TCATCGCACA TTTGATTAAT CAACAAGTTG CTCACGAAGT 3121 TCTTGCGCTG GAAATCATGA TTCTGATGCT TGAAGAACCA ACTGATGATT CAGTTGAAGT 3181 CGCCATTGCG TTCCTGAAAG AGTGTGGAGC AAAGCTTCTG GAGATTGCTC CAGCAGCTCT 3241 TAACAGTGTC TACGACCGTC TTCGTGCAAT TCTCATGGAA ACTGAAAGAT CGGAAAATGC 3301 ACTGGATCGA CGTATTCAGT ATATGATTGA GACTGCAATG CAGATTCGAA AGGACAAATT 3361 TGCGgtaagg tagaatatat aaatagttta ttagaaaaaa ataaattaga ataatttaaa 3421 ttcctactag ccaatcaggc gacctttttg cgcatagttc tattattgaa aaatttggag 3481 aatttctcat attctcgctc ggaaatctgg aattcgacga gatcttctgg cttctgtgca 3541 gctgcatcgc tttgtgctcc ctttctcgct tgtcttctgt gtacaccaag aaccttgttg 3601 agttcatcaa ctgaatctgt gactggcttg ttgctcactg gatgcactag acgactgatt 3661 ctcgagaaat cagattgagt tgcgattagg gtgacctaga aattgggaat aatacgaact 3721 tttgaaaata ttcaggagga ttaaaaaaat tattctcgac aatcctacaa atttacttat 3781 tgcaccatgt tgctccaaca tttttcatta aaagttaatg aaaaaatgta gaaaatcgga 3841 aattggcaat tttcagacca tttttaagca ttttcaaaaa aaaattgcag ctgaaataaa 3901 tgtcattttc agataaatcg agcgattttc tgttgtctga cactagtttt tagttttaaa 3961 aaatgttgga agaacatggt gcaataggta atttcataga atttccatgt gttttttttc 4021 aattaaccaa ttatccaaat cttccaaact cacattttgc ggagctgggc tatcaagaat 4081 ctgctgcagt tttataagac gagcatctct gatatcactg aaaattaatt tttaatcaaa 4141 acttgaatat caactaaacc cacttattaa ctttctcgat cttctgtcgt tcggtacgat 4201 gacggtgaag aagccaattg tagtagttga tttggttcaa gtcctttcgg tgttgtacgt 4261 cagtgtcctg caatgctatt tagttataac ttaggcctaa gattcaattt aatgaagtga 4321 ttaaatttgt tctctgaacc tcttaagatg atcttttgga ttagaaacat ataagacagg 4381 tttacctatc tattaaaaaa cagatcaaaa tagatacgac caaatcggat aatccatgcc 4441 tacctggcat ctaggaacgt gttcttagaa gatttcttac gtaatcgtat gaagaaataa 4501 caatttgatc gttggccagc aaaaataggg ttttaagtgg gatagtgttt ttattagcta 4561 accggaaaat tttatagttt ttttttgcaa gaaaccactg aaaaccccct aattgtatac 4621 attttttgga gcagcttctg gtctttttga gcaataaaat tcgataaaac agaatttaag 4681 tgtaaattgt tcacatttag tttctatttt atcaaatttt gttgctcaaa aacattcgaa 4741 gctgctctaa aaaaatgcat taaaaaaggg gttttcagtg gtttttcaca ttaaaaaagc 4801 taattttaac taaaaatcca tcatatttcc aactttgtca caacaataaa atgctggtca 4861 aaatgtgttc gaaaaaatgt tttttttttt aatttttata atttaaaaat agttttcttt 4921 cgctgggaca catacatttt tgggcgtaaa ttttcagttc aaatttccat ttttacaacc 4981 ataatcataa agctacgtct gatctctctc gcacttacct gcgcctgatt cgaaagaaca 5041 accgtagcca aaagaacaag aagaacaagc acgtagttgt ggtagtggac gttcatcacg 5101 caatactgac caatggtcgt ggggtctcac tttccgtact attgagagag gggagactga 5161 agatggcaat tgaggacagt gtcttcgacg cacgcatgca tccataagca taatccagga 5221 gggatggaga gaaaaatctt gtttctaagc ccctcccttt gtaatacata cacatatcta 5281 ataccgaaga atggctaatt gaatggacgt cagctgttgc tgtagttgcc aaggcatcat 5341 cgatgaaata actgaaagaa agaattaaat aattattgca gGCGTATCCG GCGGTCATTG 5401 AAGACTTGGA CTTGATTGAG GAGGAGGATC AGATCATCCA TACACTTAAT TTGGAGGATG 5461 CGGTTGATCC GGAAAATGGG CTTAgtaagt gactgaccac acgcgggggg cattaattta 5521 ataaattgaa ttccatttca gATGTGTTCA AACTAGATCC AGAATTCGAA AAGAACGAGG 5581 AGGTTTATGA GGAGATCCGT AAGGAAATCA TTGGAAACGC CGATATTTCG GATGAGGATG 5641 GTGGCGACGA GTTGGATGAT GAAGAAGAGG GTAGTGATGT GGAAGAGGCT CCGAAGAAGA 5701 CTACAGAGAT TATTGATAAT ACTGATCAGA ATTGACTGCT TTCAGAAGgt attcattttg 5761 agttttgggc cggcaaatct gtaagttgcc ggttgccgaa aatttgctga atttgccgga 5821 aaaaaaaatt ccggaattta tttaaaaact ttttgtaaaa attaaattaa atttgcaact 5881 tttcagAGAA GTCTACCTGA CAATGCAATC ATCTTTGGAC TACCAAGAAG CTGCTCACAA 5941 ATTGCTGAAA ATGAAGATTC CAGACAGCAT GCAGgtcagc gatgttgcaa agaaaaattt 6001 tcgaccaaaa aaaccaacca atcataaaat ttaaaaaaaa actccgtttt tttctttttt 6061 tttatacgag aaaaaccaaa aaaatgtatt tttgccaaat tctaaaatac tatccccgaa 6121 attttcaata ttttctcttt cagAACGAAC TCTGCGCGAT GCTTGTCGAT TGTTGTGCTC 6181 AACAGCGTAC CTACGAGCGA TTCTACGGAA TGCTCATCGA ACGTTTCTGC CGACTTCGCC 6241 TCGAATACCA GCAATACTTT GAAAAGCTCT GCCAGGACAC GTATTCCACG ATTCACCGAA 6301 TTGACATCAC AAAACTGCGG AATTTGGCTC GCCTTATTGC TCATTTGCTC TCGACGGATG 6361 CTATTGACTG GAAGATTTTG GCCGATATGA AAATGACCGA AGAGGACACA ACTTCTTCTG 6421 GCAGAATCTA TATTAAATAT ATATTTAATG AACTTGTGGA GGCGATGGGA ATGGTTAAAC 6481 TTCATTCGAG AGTTACTGAT CCgtgagttt cctagagaga gttgttttcg tattcaattt 6541 tccctatttt cagAACTTTG GCTCATTGCT TTGTTGGATT ATTCCCACGA ACTAATCCGA 6601 ACAGCGCACG ATTTTCGATC AACTTCTTCA CAATGATTGG ATTGGGTGGT TTGACGTTGG 6661 AACTTCGTGA ATGGCTGGCA AAGGGTCTCA AGAAGAAGAA GGGAATGCTG GATCAGTTGA 6721 AGGCCGAATC AAGCTCAGAT TCATCGTCGT CTTCGGATTC GTCAGACTCG TCTGATTCTT 6781 CGGATTCTGA CGATTCATCC GACTCGTCTT CAGATTCCTC ATCTTCTTCA GAATCAGAGC 6841 CAGAACCACC GAAGAAAAAG AAGAAGAAGA ACAGTGAAGA GAGTTCCAAA AAGAAGGAAA 6901 AAGAGAATAT TGGTCGACGG GATCGTGGAG ACAAGAGAGC TGAACGTCAT CGTGATCAAA 6961 GTGTGGAGAA CAAGGACAAG GATCGTCGAC GTCGCCAGGA TTCTGACGAA AATCGTCGGC 7021 CAGAACGAGG AGATGACCGC AAGGATCGGA GTAAAGATCG TCGTCGTCAA GACTCGGATG 7081 ATGAGGATCG GAAAGGTCGT GAACGTCGGG AAGATTCAGG GGAAAGACGT CGCGGAGATC 7141 GGGATCGACG TGATCGAAAC AAGGATCAGG AGGATCACCG TGAAGATCGC CGTGACCGAA 7201 GCAAGGATCG TGAGGATCGA CGTGATCGCC GTCGTCATGA CTCTGATGAT GATCGTAAAA 7261 CTCGTCGGGA TAGAAGTGAA GAGCGAGGAG GACGTCGTCG TGAAGTGGAA TCGGATGATC 7321 GACGCCGACG TCGTTGAATT TTCAAATTTT AAATACTGAA TATTTGTTTT TTTTCCTATT 7381 ATTTATTTAT TCTCTTTGTG TTTTTTTTCT TGCTTTCTAA AAAATTAATT CAATCCAAAT 7441 CTAAacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac 7501 acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg 7561 tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac 7621 aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga 7681 ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg 7741 cttggcaaat gcgaatccgt atggagaggc acgtttggcg aaggcaaatg ttcggtatgg 7801 agatctgtaa aaatttttaa gttgaaattt ggtgttgctc ttttacaaaa ttttccgatt 7861 ttcgcttgaa attacggtgc caggtctcga cacgtcttcc aatttttcaa attcaaaaga 7921 gcctttaatg ggctgtagtt gctaatttct cgtttttgaa aatttttctt ccgtttaatc 7981 gaaatttgat gtattttatt tatgattttc aataaatttc aaagaaactg gtgaaaactc 8041 ggaaaattgt gaactacagt aatccaatcc ttaaaggcgc acacctttta aatgtccgcc 8101 ccaatacgat atttttttaa gattcgCTAG AGCGGCCCAA AAGGCCTTTT TTTTTTGGCG 8161 CCGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 8221 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 8281 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 8341 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 8401 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 8461 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 8521 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 8581 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 8641 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 8701 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 8761 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 8821 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 8881 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 8941 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 9001 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 9061 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 9121 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 9181 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 9241 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 9301 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 9361 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 9421 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 9481 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 9541 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 9601 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 9661 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 9721 CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 9781 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 9841 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 9901 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 9961 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 10021 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 10081 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 10141 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 10201 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 10261 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 10321 CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat tctcatttaa 10381 attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT AACAACTTGG 10441 AAATGAAAT // 1589 LOCUS ADDGENE_1589 8375 bp DNA circular SYN 01/03/2005 DEFINITION L3774 complete plasmid sequence KEYWORDS . SOURCE L3774 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1589 at www.addgene.org FEATURES Location/Qualifiers source 1..8375 /organism="Addgene plasmid L3774" /mol_type="other DNA" misc_feature 50..72 /label="mec7.-836" misc_feature 862..885 /label="mec7.Tsa" promoter complement(940..958) /label="T7_promoter" CDS 975..1883 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIAADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 1146..1196 /label="SynIVS.R" misc_feature 1197..1216 /label="gfp.F64L" misc_feature 1218..1237 /label="gfp.S65T" misc_feature 1351..1401 /label="SynIVS.S" misc_feature 1524..1547 /label="gfp.M153A" misc_feature 1566..1616 /label="SynIVS.T" misc_feature 1881..1934 /label="let858.smgUTR" CDS 4559..5263 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 5239..5263 /label="let858.C" misc_feature 5341..5370 /label="let858.3'" promoter 6155..6183 /label="Amp_promoter" CDS 6225..7085 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6225..7085 /label="Ampicillin" /gene="Ampicillin" rep_origin 7240..7859 /label="pBR322_origin" misc_feature 8075..8101 /label="lacI.C" promoter 8168..8197 /label="lac_promoter" misc_feature 8232..8250 /label="M13r_primer" misc_feature 8264..8314 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGCCAA ATGTAAACCT 61 GTCATTTCTG tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagCAACAT CCGCGAGATC 901 GTGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 961 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 1021 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 1081 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 1141 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 1201 CACTTGTCAC TACTCTCACT TATGGTGTTC AATGCTTCTC gAGATACCCA GATCATATGA 1261 AACgGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 1321 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 1381 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 1441 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 1501 AATACAACTA TAACTCACAC AATGTATACA TCGCAGCAGA CAAACAAAAG AATGGAATCA 1561 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 1621 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 1681 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 1741 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1801 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 1861 TCGCTAGCCG GCCATACAAG TAATccgggt tctagaagcg ctgctaaggg ggcccTCGTC 1921 GAGTCGGTCA CAATCACCTG AAACTCCAAA GGCAGCCAGT GAGGAACGTG AAGAAGAAGA 1981 AAAAGAGTCA TCTGAACAGg tttgattttc tttctggtca aaaagatgaa attattgatt 2041 ttcagCCAGA TACTCCCAAA ACTAGCAGCG AGAAGTCTGC AAGTCGTTCA CAGTCGCCCA 2101 GAGAATCGCG GGAAGTGAGC CAAGAGgtat gtttttcaaa aatcaataac tgatcataat 2161 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat 2221 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat 2281 ttcagACTGA AACTTCGGAA AATCAAGAGA AAATCAAAGA AAAGGATGAC GGGGATGATC 2341 AGCCTGGCAC ACCGAACAGC TATAGAAGCC GGGAAACTTC ACCAGCTCCA AAAAGGTCCA 2401 AGGAGACCAG gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagAGAA 2461 TCAGAGTCTC CTGAAAAATC CCCGGTTCGT TCAAGATCTC CCAGAAGGTC TTCAGCACGT 2521 TCCCCGTCAC GATCTCCTAG ACGGCGCCGA GAAAGAAGCT CAGAAAGAAA GCAATCCGAA 2581 GAGCCAGCAC CGCTACCAGA GAAAAAGAAG AAAGAGCCGC TGGATATTCT ACGAACAAGA 2641 ACCGGAGGAG CATATATTCC ACCCGCCAAA CTTCGACTTA TGCAACAACA GATTAGTGAT 2701 AAGCAAAGTG AACAGTATCA GAGAATGAAT TGGGAAAGAA TGAAGAAAAA GATTCACGGA 2761 TTGGTTAACA GAGTCAACGC GAAGAATCTT GTTCAAATTG TCAGAGAACT TCTTCAAGAG 2821 AATGTGATTC GTTCAAAgtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt 2881 tttcagGGGA CTTCTCTGCC GTGACATTAT TCAAGCTCAG GCTTTCTCAC CAGGATTCTC 2941 TAACGTCTAT GCAGCTTTGG CGGCAGTTAT CAACTCGAAA TTCCCTCATG TCGGTGAACT 3001 TCTTCTCCGT CGTCTGATTG TACAGTTCAA AAGAAGTTTC CGTAGAAATG ACAGAGGCGT 3061 CACGGTGAAC GTGATCAAAT TCATCGCACA TTTGATTAAT CAACAAGTTG CTCACGAAGT 3121 TCTTGCGCTG GAAATCATGA TTCTGATGCT TGAAGAACCA ACTGATGATT CAGTTGAAGT 3181 CGCCATTGCG TTCCTGAAAG AGTGTGGAGC AAAGCTTCTG GAGATTGCTC CAGCAGCTCT 3241 TAACAGTGTC TACGACCGTC TTCGTGCAAT TCTCATGGAA ACTGAAAGAT CGGAAAATGC 3301 ACTGGATCGA CGTATTCAGT ATATGATTGA GACTGCAATG CAGATTCGAA AGGACAAATT 3361 TGCGGCGTAT CCGGCGGTCA TTGAAGACTT GGACTTGATT GAGGAGGAGG ATCAGATCAT 3421 CCATACACTT AATTTGGAGG ATGCGGTTGA TCCGGAAAAT GGGCTTAATG TGTTCAAACT 3481 AGATCCAGAA TTCGAAAAGA ACGAGGAGGT TTATGAGGAG ATCCGTAAGG AAATCATTGG 3541 AAACGCCGAT ATTTCGGATG AGGATGGTGG CGACGAGTTG GATGATGAAG AAGAGGGTAG 3601 TGATGTGGAA GAGGCTCCGA AGAAGACTAC AGAGATTATT GATAATACTG ATCAGAATTG 3661 ACTGCTTTCA GAAGgtattc attttgagtt ttgggccggc aaatctgtaa gttgccggtt 3721 gccgaaaatt tgctgaattt gccggaaaaa aaaattccgg aatttattta aaaacttttt 3781 gtaaaaatta aattaaattt gcaacttttc agAGAAGTCT ACCTGACAAT GCAATCATCT 3841 TTGGACTACC AAGAAGCTGC TCACAAATTG CTGAAAATGA AGATTCCAGA CAGCATGCAG 3901 gtcagcgatg ttgcaaagaa aaattttcga ccaaaaaaac caaccaatca taaaatttaa 3961 aaaaaaactc cgtttttttc ttttttttta tacgagaaaa accaaaaaaa tgtatttttg 4021 ccaaattcta aaatactatc cccgaaattt tcaatatttt ctctttcagA ACGAACTCTG 4081 CGCGATGCTT GTCGATTGTT GTGCTCAACA GCGTACCTAC GAGCGATTCT ACGGAATGCT 4141 CATCGAACGT TTCTGCCGAC TTCGCCTCGA ATACCAGCAA TACTTTGAAA AGCTCTGCCA 4201 GGACACGTAT TCCACGATTC ACCGAATTGA CATCACAAAA CTGCGGAATT TGGCTCGCCT 4261 TATTGCTCAT TTGCTCTCGA CGGATGCTAT TGACTGGAAG ATTTTGGCCG ATATGAAAAT 4321 GACCGAAGAG GACACAACTT CTTCTGGCAG AATCTATATT AAATATATAT TTAATGAACT 4381 TGTGGAGGCG ATGGGAATGG TTAAACTTCA TTCGAGAGTT ACTGATCCgt gagtttccta 4441 gagagagttg ttttcgtatt caattttccc tattttcagA ACTTTGGCTC ATTGCTTTGT 4501 TGGATTATTC CCACGAACTA ATCCGAACAG CGCACGATTT TCGATCAACT TCTTCACAAT 4561 GATTGGATTG GGTGGTTTGA CGTTGGAACT TCGTGAATGG CTGGCAAAGG GTCTCAAGAA 4621 GAAGAAGGGA ATGCTGGATC AGTTGAAGGC CGAATCAAGC TCAGATTCAT CGTCGTCTTC 4681 GGATTCGTCA GACTCGTCTG ATTCTTCGGA TTCTGACGAT TCATCCGACT CGTCTTCAGA 4741 TTCCTCATCT TCTTCAGAAT CAGAGCCAGA ACCACCGAAG AAAAAGAAGA AGAAGAACAG 4801 TGAAGAGAGT TCCAAAAAGA AGGAAAAAGA GAATATTGGT CGACGGGATC GTGGAGACAA 4861 GAGAGCTGAA CGTCATCGTG ATCAAAGTGT GGAGAACAAG GACAAGGATC GTCGACGTCG 4921 CCAGGATTCT GACGAAAATC GTCGGCCAGA ACGAGGAGAT GACCGCAAGG ATCGGAGTAA 4981 AGATCGTCGT CGTCAAGACT CGGATGATGA GGATCGGAAA GGTCGTGAAC GTCGGGAAGA 5041 TTCAGGGGAA AGACGTCGCG GAGATCGGGA TCGACGTGAT CGAAACAAGG ATCAGGAGGA 5101 TCACCGTGAA GATCGCCGTG ACCGAAGCAA GGATCGTGAG GATCGACGTG ATCGCCGTCG 5161 TCATGACTCT GATGATGATC GTAAAACTCG TCGGGATAGA AGTGAAGAGC GAGGAGGACG 5221 TCGTCGTGAA GTGGAATCGG ATGATCGACG CCGACGTCGT TGAATTTTCA AATTTTAAAT 5281 ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT 5341 TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt ttctctttcc gtctcccaat 5401 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca 5461 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa 5521 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa 5581 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat 5641 taggcgaacg atgagaattg tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt 5701 ttggcgaagg caaatgttcg gtatggagat ctgtaaaaat ttttaagttg aaatttggtg 5761 ttgctctttt acaaaatttt ccgattttcg cttgaaatta cggtgccagg tctcgacacg 5821 tcttccaatt tttcaaattc aaaagagcct ttaatgggct gtagttgcta atttctcgtt 5881 tttgaaaatt tttcttccgt ttaatcgaaa tttgatgtat tttatttatg attttcaata 5941 aatttcaaag aaactggtga aaactcggaa aattgtgaac tacagtaatc caatccttaa 6001 aggcgcacac cttttaaatg tccgccccaa tacgatattt ttttaagatt cgCTAGAGCG 6061 GCCCAAAAGG CCTTTTTTTT TTGGCGCCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 6121 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 6181 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 6241 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 6301 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 6361 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 6421 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 6481 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 6541 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 6601 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 6661 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 6721 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 6781 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 6841 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 6901 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 6961 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 7021 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 7081 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 7141 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 7201 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 7261 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 7321 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 7381 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 7441 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 7501 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 7561 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 7621 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 7681 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 7741 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 7801 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 7861 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 7921 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 7981 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 8041 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 8101 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 8161 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 8221 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat 8281 cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA 8341 CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1590 LOCUS ADDGENE_1590 3777 bp DNA circular SYN 01/03/2005 DEFINITION L3781 complete plasmid sequence KEYWORDS . SOURCE L3781 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1590 at www.addgene.org FEATURES Location/Qualifiers source 1..3777 /organism="Addgene plasmid L3781" /mol_type="other DNA" promoter complement(83..101) /label="T7_promoter" CDS 118..1026 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 118..966 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 289..339 /label="SynIVS.R" misc_feature 361..380 /label="gfp.S65C" misc_feature 494..544 /label="SynIVS.S" misc_feature 709..759 /label="SynIVS.T" misc_feature 1132..1161 /label="let858.3'" promoter 1557..1585 /label="Amp_promoter" gene 1627..2487 /label="Ampicillin" /gene="Ampicillin" CDS 1627..2487 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2642..3261 /label="pBR322_origin" misc_feature 3477..3503 /label="lacI.C" promoter 3570..3599 /label="lac_promoter" misc_feature 3634..3652 /label="M13r_primer" misc_feature 3666..3716 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGGATC CTATCGATTC 61 GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT AGAAAAAATG 121 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 181 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 241 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac 301 atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC 361 TgTTATGGTG TTCAATGCTT cTCGAGATAC CCAGATCATA TGAAACAGCA TGACTTTTTC 421 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 481 AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt 541 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT 601 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA 661 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac 721 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC 781 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT 841 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT 901 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA 961 CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCGCTAG CCGGCCATAC 1021 AAGTAATCCG GATGATCGAC GCCaACGTCG TTGAATTTTC AAATTTTAAA TACTGAATAT 1081 TTGTTTTTTT TCCTATTATT TATTTATTCT CTTTGTGTTT TTTTTCTTGC TTTCTAAAAA 1141 ATTAATTCAA TCCAAATCTA Aacatttttt tttctctttc cgtctcccaa ttcgtattcc 1201 gctcctctca tctgaacaca atgtgcaagt ttatttatct tctcgctttc atttcattag 1261 gacgtggggg gaattggtgg aagggggaaa cacacaaaag gatgatggaa atgaaataag 1321 gacacacaat atgcaacaac attcaattca gaaatatgga ggaaggttta aaagaaaaca 1381 taaaaatata tagaggagga aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac 1441 gatgAGAATT GTCCTCGCTT GGGCCCAAAA GGCCTTTTTT TTTTGGCGCC GACGTCAGGT 1501 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 1561 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG 1621 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 1681 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 1741 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 1801 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 1861 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 1921 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 1981 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 2041 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 2101 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 2161 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 2221 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 2281 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 2341 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 2401 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 2461 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG 2521 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 2581 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA 2641 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA 2701 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 2761 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG 2821 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 2881 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 2941 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 3001 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC 3061 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 3121 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 3181 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 3241 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT 3301 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG 3361 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA 3421 GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC 3481 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG 3541 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG 3601 TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC 3661 AAGCTgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagAGCT 3721 TAAAAATGGC TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1591 LOCUS ADDGENE_1591 5649 bp DNA circular SYN 01/03/2005 DEFINITION L3784 complete plasmid sequence KEYWORDS . SOURCE L3784 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1591 at www.addgene.org FEATURES Location/Qualifiers source 1..5649 /organism="Addgene plasmid L3784" /mol_type="other DNA" misc_feature 12..35 /label="mec3.-1822" misc_feature 1834..1857 /label="mec3.Tsa" promoter complement(1955..1973) /label="T7_promoter" CDS 1990..2898 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 1990..2838 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2161..2211 /label="SynIVS.R" misc_feature 2233..2252 /label="gfp.S65C" misc_feature 2366..2416 /label="SynIVS.S" misc_feature 2581..2631 /label="SynIVS.T" misc_feature 3004..3033 /label="let858.3'" promoter 3429..3457 /label="Amp_promoter" CDS 3499..4359 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3499..4359 /label="Ampicillin" /gene="Ampicillin" rep_origin 4514..5133 /label="pBR322_origin" misc_feature 5349..5375 /label="lacI.C" promoter 5442..5471 /label="lac_promoter" misc_feature 5506..5524 /label="M13r_primer" misc_feature 5538..5588 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT ACCCGGAGTA GTTGGCGAAG ATAGAAATGG TGTTATCTTA 61 TATAAATTCA GACTTTCTCT GTTAAAATTT TAACCACCAA AAAATATAGA ATTTATAAAA 121 AGTTTCATTT TGAAATTTTA TCTTTTGGGG CCATTTCACT ACCTTATCAG GAAGAAATGT 181 GTTTCATCAG AAATTGAATT TTTCATCTTT TCAGAAAGTG TACCTAATAC CGTATCATGC 241 TCATATTCAC GTGAATAAGG CTTCTGCCCA GTTCTGTGAT TCAAGGAAAA CTACGAAAAA 301 TGATCTCATT TTCTTTGATT TATCACCGAG CTGGTGTGTC ATCACACACA GTCTGTTTCT 361 TGATAAAAAC GCGCAGAAGT TAGAGCATTC ATTGTGATTT TGGGTCTTTA ATTTCTGTAA 421 AACAAATAAA GTGACAAGTT TATCATCTGC CTATTTTATC AGTTATATGC ATGGTTAGTT 481 GCAAATAAGG GAGTAGAAAT TGACTAAAAC TCAAAATGAG CACTGAAACG AGGGGTTTTG 541 TTAGTTGCGA AATAACAATT AATACAATCT GGTTGATTAC TTTGTAAAAG GATAATAAAA 601 TATGCAAAAC TTGGCGTATT CTGAGATTTC ATTCCTAAAG ACATTTATCA AAACAAATGT 661 TTTCTGCAAT TTTCTAAATT CTTGGAATTC TGCCCAGCAG CCTCTAATAT CGCGTTTTAC 721 TTAGAAAATG ACTTTTGGTT TCATTTTCTC ATTTCTGGCT TCTATTTTTC ACAAATGCAA 781 ATATTACATT TAAATTAGTA CTTTCATTTC TACAAAGATC AAATTTAACA TTATTGACAG 841 GTTTACTGTT ATCTTGTGAC AGTTCCGTAC TGTCAATCAA AACGAAAATT AAAACTCATG 901 AAATATCAAT GAACATAAAT TGAAAAAAAA ACCCAGTTGA AAGGAGAAAA TAAGCGAAAC 961 ACACCAACAA CTGAGTAGTT ACGCTCACAA TTTGGAAATG ATTTTGAAAA CTCTCTGGAC 1021 TGCATTTCAA TTTTTAACAA ACAATGAATA CATACTGATA GTTTTTGTAC TTCTCGGCAT 1081 TCAGAGGCAA CTTCCTGCCA ACAACCTGGC AACTTATTTA GTGCCACTAA AATAAGTTGC 1141 CAAATTGTTG GCAGGAAGTT GCCTCCAAAT GCCGAGTTGG AGCAAATGAT TCGATATGAT 1201 TTTAAGGATA CCTACGCATG CCTCCTGCCA AGACACGCAC CCACCTACTG AAATGTCGCG 1261 GAATTTTACC TCCCAAACTA TAGATTGGGT GAAACTTCAC TGTAAAAGCT TTTTTTAAGT 1321 AGTAGGCTGG CAGTAATGAA GACGTCCATA CCATAATTTT TAATGTTGTA TAGAAAATCT 1381 GAATTCTCAA GTATAACATT TTCCAATAAT TAGCATAGTC AGAAACTCTC CACCGCCGTT 1441 CCGTTCCTTT TTTTTGTAAC TATGGAGTAA TAGAACAAGA TTATTCAAAT TCAAATTTTT 1501 ATAAAACTTT ACAACGTCCT TTCCATATAT TCTTCACACA ACATTTGAAA AAACAACAAA 1561 TTCATTCGAA ATGCATTGCC CATAATGAAT CGACCGAAAA ACACAAGTGA CCGTCAGGAG 1621 ATCGATAGAG AGAGCCCGTT CCAACTAGAC AACTTTTTAG TGCTTATCCT TACACACACT 1681 TTCTAGCTTC ATAAGAAATG CATCTATTAT CGTCACCATT TGGAGACACC AGTTTTAGCG 1741 CACATTAATA ATCGATCAGG GTCTAGAGAC TCCTGTTGGA TTGGCATGCC ACGCCTACAT 1801 GACATCTGGC TCTTGACGGG TGATTTGTTA CAGTGAGTAG CCACAATTTC GCGCATTTTC 1861 TTCTAAATTT CACTGTTATT TGTTCTTctg atttatttca tttgaactac ggaaatcgGA 1921 TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT AGTGAGTCGT ATTGGTACCG 1981 GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA 2041 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA 2101 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG 2161 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gCCAACACTT 2221 GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCGAGAT ACCCAGATCA TATGAAACAG 2281 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC 2341 AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa ctaaccatac 2401 atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATAGAATC 2461 GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA ATTGGAATAC 2521 AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT 2581 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gAACTTCAAA 2641 ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA ACAAAATACT 2701 CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC ACAATCTGCC 2761 CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT TGTAACAGCT 2821 GCTGGGATTA CACATGGCAT GGAcGAACTA TACAAAagcc cacaAGCTTT GGAATTCGCT 2881 AGCCGGCCAT ACAAGTAATC CGGATGATCG ACGCCaACGT CGTTGAATTT TCAAATTTTA 2941 AATACTGAAT ATTTGTTTTT TTTCCTATTA TTTATTTATT CTCTTTGTGT TTTTTTTCTT 3001 GCTTTCTAAA AAATTAATTC AATCCAAATC TAAacatttt tttttctctt tccgtctccc 3061 aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt 3121 tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg 3181 aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt 3241 taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga 3301 aattaggcga acgatgAGAA TTGTCCTCGC TTGGGCCCAA AAGGCCTTTT TTTTTTGGCG 3361 CCGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 3421 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 3481 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 3541 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 3601 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 3661 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 3721 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 3781 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 3841 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 3901 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 3961 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 4021 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 4081 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 4141 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 4201 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 4261 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 4321 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 4381 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 4441 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 4501 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 4561 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 4621 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 4681 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 4741 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 4801 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 4861 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 4921 CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 4981 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 5041 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 5101 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 5161 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 5221 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 5281 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 5341 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 5401 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 5461 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 5521 CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat tctcatttaa 5581 attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT AACAACTTGG 5641 AAATGAAAT // 1592 LOCUS ADDGENE_1592 6419 bp DNA circular SYN 01/03/2005 DEFINITION L3785 complete plasmid sequence KEYWORDS . SOURCE L3785 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1592 at www.addgene.org FEATURES Location/Qualifiers source 1..6419 /organism="Addgene plasmid L3785" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2440..2458) /label="T7_promoter" gene 2475..3323 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2475..3383 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 2646..2696 /label="SynIVS.R" misc_feature 2718..2737 /label="gfp.S65C" misc_feature 2851..2901 /label="SynIVS.S" misc_feature 3066..3116 /label="SynIVS.T" misc_feature 3489..3518 /label="let858.3'" promoter 4199..4227 /label="Amp_promoter" CDS 4269..5129 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4269..5129 /label="Ampicillin" /gene="Ampicillin" rep_origin 5284..5903 /label="pBR322_origin" misc_feature 6119..6145 /label="lacI.C" promoter 6212..6241 /label="lac_promoter" misc_feature 6276..6294 /label="M13r_primer" misc_feature 6308..6358 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 2461 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 2521 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 2581 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 2641 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 2701 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA 2761 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 2821 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 2881 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 2941 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 3001 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 3061 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 3121 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 3181 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 3241 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 3301 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 3361 TCGCTAGCCG GCCATACAAG TAATCCGGAT GATCGACGCC aACGTCGTTG AATTTTCAAA 3421 TTTTAAATAC TGAATATTTG TTTTTTTTCC TATTATTTAT TTATTCTCTT TGTGTTTTTT 3481 TTCTTGCTTT CTAAAAAATT AATTCAATCC AAATCTAAac attttttttt ctctttccgt 3541 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct 3601 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat 3661 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga 3721 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc 3781 aaagaaatta ggcgaacgat gAGAATTGTC CTCGCTTGGG CCCTTTCGTC TCGCGCGTTT 3841 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT 3901 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG 3961 TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG 4021 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC 4081 TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG 4141 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 4201 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 4261 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 4321 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 4381 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 4441 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 4501 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 4561 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 4621 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 4681 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 4741 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 4801 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 4861 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 4921 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 4981 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 5041 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 5101 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 5161 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 5221 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 5281 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 5341 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 5401 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC 5461 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 5521 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 5581 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 5641 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA 5701 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 5761 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 5821 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 5881 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 5941 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG 6001 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG 6061 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT 6121 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 6181 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT 6241 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG 6301 CCAAGCTgta agtttaaaca tgatcttact aactaactat tctcatttaa attttcagAG 6361 CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT AACAACTTGG AAATGAAAT // 1593 LOCUS ADDGENE_1593 7514 bp DNA circular SYN 01/03/2005 DEFINITION L3786 complete plasmid sequence KEYWORDS . SOURCE L3786 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1593 at www.addgene.org FEATURES Location/Qualifiers source 1..7514 /organism="Addgene plasmid L3786" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" promoter complement(3535..3553) /label="T7_promoter" CDS 3570..4478 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 3570..4418 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3741..3791 /label="SynIVS.R" misc_feature 3813..3832 /label="gfp.S65C" misc_feature 3946..3996 /label="SynIVS.S" misc_feature 4161..4211 /label="SynIVS.T" misc_feature 4584..4613 /label="let858.3'" promoter 5294..5322 /label="Amp_promoter" gene 5364..6224 /label="Ampicillin" /gene="Ampicillin" CDS 5364..6224 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6379..6998 /label="pBR322_origin" misc_feature 7214..7240 /label="lacI.C" promoter 7307..7336 /label="lac_promoter" misc_feature 7371..7389 /label="M13r_primer" misc_feature 7403..7453 /label="decoy" misc_feature 7511..7534 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtggtacc gctagccgta 3481 cgaacccggg ttCTAGAGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 3541 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 3601 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 3661 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 3721 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 3781 taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCGAGAT 3841 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 3901 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 3961 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 4021 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 4081 TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC 4141 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 4201 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 4261 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 4321 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 4381 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGAcGAACTA TACAAAagcc 4441 cacaAGCTTT GGAATTCGCT AGCCGGCCAT ACAAGTAATC CGGATGATCG ACGCCaACGT 4501 CGTTGAATTT TCAAATTTTA AATACTGAAT ATTTGTTTTT TTTCCTATTA TTTATTTATT 4561 CTCTTTGTGT TTTTTTTCTT GCTTTCTAAA AAATTAATTC AATCCAAATC TAAacatttt 4621 tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa 4681 gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga 4741 aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt 4801 cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac 4861 tagtaaaaaa taagcaaaga aattaggcga acgatgAGAA TTGTCCTCGC TTGGGCCCTT 4921 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4981 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 5041 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 5101 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 5161 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 5221 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 5281 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 5341 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 5401 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 5461 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 5521 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 5581 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 5641 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 5701 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 5761 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5821 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5881 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5941 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 6001 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 6061 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 6121 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 6181 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 6241 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 6301 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 6361 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 6421 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 6481 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 6541 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 6601 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 6661 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 6721 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6781 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6841 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6901 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6961 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 7021 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 7081 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 7141 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 7201 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 7261 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 7321 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 7381 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 7441 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 7501 CTTGGAAATG AAAT // 1594 LOCUS ADDGENE_1594 4163 bp DNA circular SYN 01/03/2005 DEFINITION L3787 complete plasmid sequence KEYWORDS . SOURCE L3787 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1594 at www.addgene.org FEATURES Location/Qualifiers source 1..4163 /organism="Addgene plasmid L3787" /mol_type="other DNA" misc_feature complement(115..139) /label="hsp16-41.5'" misc_feature 353..377 /label="hsp16-2.5'" promoter complement(469..487) /label="T7_promoter" CDS 504..1412 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 504..1352 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 675..725 /label="SynIVS.R" misc_feature 747..766 /label="gfp.S65C" misc_feature 880..930 /label="SynIVS.S" misc_feature 1095..1145 /label="SynIVS.T" misc_feature 1518..1547 /label="let858.3'" promoter 1943..1971 /label="Amp_promoter" CDS 2013..2873 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2013..2873 /label="Ampicillin" /gene="Ampicillin" rep_origin 3028..3647 /label="pBR322_origin" misc_feature 3863..3889 /label="lacI.C" promoter 3956..3985 /label="lac_promoter" misc_feature 4020..4038 /label="M13r_primer" misc_feature 4052..4102 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCAAGAG CATTTGAATC AGAATATGGA 61 GAACGGAGCA TGAGCATTTT CGAAGTTTTT TAGATGCACT AGAACAAAGC GTGTTGGCTT 121 CCTCTGAGCC CGCTTTCCTT ATATACCCGC ATTCTGCAGC CTTACAGAAT GTTCTAGAAG 181 GTCCTAGATG CATTCGTTTG AAAATACTCC CGGTGGGTGC AAAGAGACGC AGACGGAAAA 241 TGTATCTGGG TCTCTTTATT GTGTACACTA CTTTTCCATG TACCGAATGT GAGTCGCCCT 301 CCTTTTGCAA CAAGCAGCTC GAATGTTCTA GAAAAAGGTG GAAAATAGTA TAAATACCGT 361 TGAAAATAAA TACCGAACAA CATTTGCTCT AATTGTGAAA TTAGAAATCT TCAAACTATA 421 ATCATctcac tgGATCCTAT CGATTCGCGG CCGCTGTACA CCCGGGTGCC CTATAGTGAG 481 TCGTATTGGT ACCGGTAGAA AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC 541 CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG 601 GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC 661 TACCTGTTCC ATGGgtaagt ttaaacatat atatactaac taaccctgat tatttaaatt 721 ttcagCCAAC ACTTGTCACT ACTTTCTgTT ATGGTGTTCA ATGCTTcTCG AGATACCCAG 781 ATCATATGAA ACAGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA 841 GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACg taagtttaaa cagttcggta 901 ctaactaacc atacatattt aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC 961 TTGTTAATAG AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC 1021 ACAAATTGGA ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA 1081 ATGGAATCAA AGTTgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt 1141 ttcagAACTT CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT 1201 ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT 1261 CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG 1321 AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGAcGA ACTATACAAA agcccacaAG 1381 CTTTGGAATT CGCTAGCCGG CCATACAAGT AATCCGGATG ATCGACGCCa ACGTCGTTGA 1441 ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT ATTATTTATT TATTCTCTTT 1501 GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA AATCTAAaca tttttttttc 1561 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat 1621 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca 1681 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa 1741 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa 1801 aaaataagca aagaaattag gcgaacgatg AGAATTGTCC TCGCTTGGGC CCAAAAGGCC 1861 TTTTTTTTTT GGCGCCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 1921 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 1981 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 2041 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 2101 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 2161 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 2221 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 2281 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 2341 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 2401 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 2461 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 2521 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 2581 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 2641 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 2701 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 2761 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 2821 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 2881 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 2941 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 3001 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 3061 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 3121 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 3181 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 3241 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 3301 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 3361 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 3421 ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 3481 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 3541 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 3601 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 3661 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 3721 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 3781 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC 3841 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 3901 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC 3961 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG 4021 GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct tactaactaa 4081 ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA 4141 CGCTAACAAC TTGGAAATGA AAT // 1595 LOCUS ADDGENE_1595 4171 bp DNA circular SYN 01/03/2005 DEFINITION L3788 complete plasmid sequence KEYWORDS . SOURCE L3788 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1595 at www.addgene.org FEATURES Location/Qualifiers source 1..4171 /organism="Addgene plasmid L3788" /mol_type="other DNA" misc_feature complement(130..154) /label="hsp16-2.5'" misc_feature 368..392 /label="hsp16-41.5'" promoter complement(477..495) /label="T7_promoter" gene 512..1360 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 512..1420 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 683..733 /label="SynIVS.R" misc_feature 755..774 /label="gfp.S65C" misc_feature 888..938 /label="SynIVS.S" misc_feature 1103..1153 /label="SynIVS.T" misc_feature 1526..1555 /label="let858.3'" promoter 1951..1979 /label="Amp_promoter" CDS 2021..2881 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2021..2881 /label="Ampicillin" /gene="Ampicillin" rep_origin 3036..3655 /label="pBR322_origin" misc_feature 3871..3897 /label="lacI.C" promoter 3964..3993 /label="lac_promoter" misc_feature 4028..4046 /label="M13r_primer" misc_feature 4060..4110 /label="decoy" ORIGIN 1 AAGCTTGcat gcctgcaggt cgactctaga gGATCACCAA AAACGGAACG TTGAGCTGGA 61 CGGAAATAGT GGTAAAGTGA CATGATTATA GTTTGAAGAT TTCTAATTTC ACAATTAGAG 121 CAAATGTTGT TCGGTATTTA TTTTCAACGG TATTTATACT ATTTTCCACC TTTTTCTAGA 181 ACATTCGAGC TGCTTGTTGC AAAAGGAGGG CGACTCACAT TCGGTACATG GAAAAGTAGT 241 GTACACAATA AAGAGACCCA GATACATTTT CCGTCTGCGT CTCTTTGCAC CCACCGGGAG 301 TATTTTCAAA CGAATGCATC TAGGACCTTC TAGAACATTC TGTAAGGCTG CAGAATGCGG 361 GTATATAAGG AAAGCGGGCT CAGAGGAAGC CAACACGCTT TGTTCTAGTG CATCTAAAAA 421 ACTTCGAAAA Tcctcatcgg GATCCTATCG ATTCGCGGCC GCTGTACACC CGGGTGCCCT 481 ATAGTGAGTC GTATTGGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG 541 AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG 601 TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC 661 TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta accctgatta 721 tttaaatttt cagCCAACAC TTGTCACTAC TTTCTgTTAT GGTGTTCAAT GCTTcTCGAG 781 ATACCCAGAT CATATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT 841 ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca 901 gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG 961 TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT 1021 TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA 1081 ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta actaatctga 1141 tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC 1201 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA 1261 TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT 1321 CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGAcGAAC TATACAAAag 1381 cccacaAGCT TTGGAATTCG CTAGCCGGCC ATACAAGTAA TCCGGATGAT CGACGCCaAC 1441 GTCGTTGAAT TTTCAAATTT TAAATACTGA ATATTTGTTT TTTTTCCTAT TATTTATTTA 1501 TTCTCTTTGT GTTTTTTTTC TTGCTTTCTA AAAAATTAAT TCAATCCAAA TCTAAacatt 1561 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc 1621 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg 1681 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa 1741 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa 1801 actagtaaaa aataagcaaa gaaattaggc gaacgatgAG AATTGTCCTC GCTTGGGCCC 1861 AAAAGGCCTT TTTTTTTTGG CGCCGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 1921 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 1981 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 2041 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 2101 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 2161 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 2221 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 2281 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 2341 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 2401 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 2461 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 2521 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 2581 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 2641 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 2701 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 2761 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 2821 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 2881 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 2941 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 3001 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3061 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3121 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3181 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3241 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3301 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3361 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3421 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3481 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3541 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3601 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 3661 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 3721 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 3781 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 3841 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 3901 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 3961 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 4021 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta 4081 ctaactaact attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC 4141 GATGGATACG CTAACAACTT GGAAATGAAA T // 1596 LOCUS ADDGENE_1596 5034 bp DNA circular SYN 01/03/2005 DEFINITION L3790 complete plasmid sequence KEYWORDS . SOURCE L3790 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1596 at www.addgene.org FEATURES Location/Qualifiers source 1..5034 /organism="Addgene plasmid L3790" /mol_type="other DNA" misc_feature 20..44 /label="myo2.-1090" misc_feature 1108..1131 /label="myo2.5'" misc_feature 1205..1246 /label="SynIVS.A" misc_feature 1253..1270 /label="rf21.28" promoter complement(1340..1358) /label="T7_promoter" gene 1375..2223 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1375..2283 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 1546..1596 /label="SynIVS.R" misc_feature 1618..1637 /label="gfp.S65C" misc_feature 1751..1801 /label="SynIVS.S" misc_feature 1966..2016 /label="SynIVS.T" misc_feature 2389..2418 /label="let858.3'" promoter 2814..2842 /label="Amp_promoter" gene 2884..3744 /label="Ampicillin" /gene="Ampicillin" CDS 2884..3744 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3899..4518 /label="pBR322_origin" misc_feature 4734..4760 /label="lacI.C" promoter 4827..4856 /label="lac_promoter" misc_feature 4891..4909 /label="M13r_primer" misc_feature 4923..4973 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGAGGCATTT GAATTGGGGG TGGTGGACAG TAACTGTCTG 61 TAATAATAAT TACTCCTGAC CAGGTTGCAA TTCGAGTTTT GATAAGCATA ATTATACCTT 121 GTACATTGTG GGTTTTGTGC TGTGGACGTT TTATTGTGAC ATCCCCATAA GCTACAAGAA 181 CAAAAATGAA ATgTAAAAGT ATTGAAAACG TCGTACATTT TATATCTGAG TAGTATCCTT 241 TGCTTTAAAT GTCCATAAAA cTAATTTTAT AATCAATAAA ACAACGTTTG TAAATCAACT 301 GAGTTTACAA GTAGAGACAT TGAGGGATAC TTTCACTATG CTAAAGTGAT AATCGACCAA 361 ATAATAACTt CACTTTGGTA TTTATTCCTG TCTTATAAaT GTTATGTATG AATTAAATTC 421 ATATGCATAT GGCTCACTCT GACAAttttt AATAATCTTC CAGATCAATA TTGACTACCG 481 ATGCGGGTGG TCTTTTGCTT TGAATTCTGC TGAACTTTAC ACCCCGAACA GCAATGTGTG 541 CTTCAGCcTA AAAAAAAGTA AGTGTGTTAA TCAGTgCCCC CGATTCTTCA TTTTTTGCCC 601 CTCTCTCCCG TTTCGTCGGC AAAAGAAGAG AAAATAAAGA TAAGTCTCAA GATAGGTTGG 661 TAATCGCTAA AGTGGTTGTG TGGATAAGAG TAGCAAAATG GCAGGAAGAG CACTTTGCGC 721 GCACACACTG TACTCATTGT TCTGGATAAA ATTCTCTCGT TGTTTGCCGT CGGATGTCTG 781 CCTCTCTGCA TTGAGCCGGC TTCTTCACTA TCTTTAGTTA ACCTAAAATG CCGTTTCTTT 841 TCTCGTATCC CcACTATcCC GTTGAGGTTC TCTGCTCTCT TCGCTCCCTA CCGCCAGCGA 901 GCAACTATCC GTGGGGGCGC CTTGCTCGGA AGATGGGGGG GAAGAAAGAA GATTTTTGCT 961 ATTTGCACTT GAGAAAGAGA CTTTTCCTGC GTCGATGGTT AGAGAACAGT GTGCAGACAC 1021 TTTTCAGCTA CCTAGAatTA CAatTGGATA TCCCCGCCTC CCAATCCACC CACCCAGGGA 1081 AAAAGAAGGG CTCGCCGAAA ATCAAAGTTA TCTCCAGGCT CGCGCATCCC ACCGAGCGGT 1141 TGACTTCTCT CCACCACTTT TCATTTTAAC CCTCGGGGTA CGGGATTGGC CAAAGGACCC 1201 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGCT 1261 AGCGTCGACG GTACCGGTCA GATATCACTC GAGGAGCTCC CGAGATCCTA TCGATTCGCG 1321 GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG TACCGGTAGA AAAAATGAGT 1381 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT 1441 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT 1501 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag tttaaacata 1561 tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC TACTTTCTgT 1621 TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA AACAGCATGA CTTTTTCAAG 1681 AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA TGACGGGAAC 1741 TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt taaattttca 1801 gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT AAAAGGTATT 1861 GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA TAACTCACAC 1921 AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag tttaaacatg 1981 attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG ACACAACATT 2041 GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT TGGCGATGGC 2101 CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC 2161 AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG GATTACACAT 2221 GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT TCGCTAGCCG GCCATACAAG 2281 TAATCCGGAT GATCGACGCC aACGTCGTTG AATTTTCAAA TTTTAAATAC TGAATATTTG 2341 TTTTTTTTCC TATTATTTAT TTATTCTCTT TGTGTTTTTT TTCTTGCTTT CTAAAAAATT 2401 AATTCAATCC AAATCTAAac attttttttt ctctttccgt ctcccaattc gtattccgct 2461 cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac 2521 gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac 2581 acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa 2641 aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta ggcgaacgat 2701 gAGAATTGTC CTCGCTTGGG CCCAAAAGGC CTTTTTTTTT TGGCGCCGAC GTCAGGTGGC 2761 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 2821 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 2881 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 2941 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 3001 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 3061 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 3121 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 3181 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 3241 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 3301 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 3361 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 3421 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 3481 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 3541 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 3601 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 3661 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 3721 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 3781 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 3841 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 3901 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 3961 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 4021 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 4081 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 4141 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 4201 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 4261 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 4321 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 4381 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 4441 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 4501 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 4561 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 4621 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 4681 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 4741 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 4801 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 4861 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 4921 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 4981 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1597 LOCUS ADDGENE_1597 8375 bp DNA circular SYN 01/03/2005 DEFINITION L3799 complete plasmid sequence KEYWORDS . SOURCE L3799 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1597 at www.addgene.org FEATURES Location/Qualifiers source 1..8375 /organism="Addgene plasmid L3799" /mol_type="other DNA" misc_feature 50..72 /label="mec7.-836" misc_feature 862..885 /label="mec7.Tsa" promoter complement(940..958) /label="T7_promoter" CDS 975..1883 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 975..1823 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1146..1196 /label="SynIVS.R" misc_feature 1218..1237 /label="gfp.S65C" misc_feature 1351..1401 /label="SynIVS.S" misc_feature 1566..1616 /label="SynIVS.T" misc_feature 1881..1934 /label="let858.smgUTR" CDS 4559..5263 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 5239..5263 /label="let858.C" misc_feature 5341..5370 /label="let858.3'" promoter 6155..6183 /label="Amp_promoter" CDS 6225..7085 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6225..7085 /label="Ampicillin" /gene="Ampicillin" rep_origin 7240..7859 /label="pBR322_origin" misc_feature 8075..8101 /label="lacI.C" promoter 8168..8197 /label="lac_promoter" misc_feature 8232..8250 /label="M13r_primer" misc_feature 8264..8314 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGCCAA ATGTAAACCT 61 GTCATTTCTG tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagCAACAT CCGCGAGATC 901 GTGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 961 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 1021 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 1081 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 1141 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 1201 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA 1261 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 1321 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 1381 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 1441 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 1501 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 1561 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 1621 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 1681 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 1741 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 1801 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 1861 TCGCTAGCCG GCCATACAAG TAATccgggt tctagaagcg ctgctaaggg ggcccTCGTC 1921 GAGTCGGTCA CAATCACCTG AAACTCCAAA GGCAGCCAGT GAGGAACGTG AAGAAGAAGA 1981 AAAAGAGTCA TCTGAACAGg tttgattttc tttctggtca aaaagatgaa attattgatt 2041 ttcagCCAGA TACTCCCAAA ACTAGCAGCG AGAAGTCTGC AAGTCGTTCA CAGTCGCCCA 2101 GAGAATCGCG GGAAGTGAGC CAAGAGgtat gtttttcaaa aatcaataac tgatcataat 2161 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat 2221 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat 2281 ttcagACTGA AACTTCGGAA AATCAAGAGA AAATCAAAGA AAAGGATGAC GGGGATGATC 2341 AGCCTGGCAC ACCGAACAGC TATAGAAGCC GGGAAACTTC ACCAGCTCCA AAAAGGTCCA 2401 AGGAGACCAG gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagAGAA 2461 TCAGAGTCTC CTGAAAAATC CCCGGTTCGT TCAAGATCTC CCAGAAGGTC TTCAGCACGT 2521 TCCCCGTCAC GATCTCCTAG ACGGCGCCGA GAAAGAAGCT CAGAAAGAAA GCAATCCGAA 2581 GAGCCAGCAC CGCTACCAGA GAAAAAGAAG AAAGAGCCGC TGGATATTCT ACGAACAAGA 2641 ACCGGAGGAG CATATATTCC ACCCGCCAAA CTTCGACTTA TGCAACAACA GATTAGTGAT 2701 AAGCAAAGTG AACAGTATCA GAGAATGAAT TGGGAAAGAA TGAAGAAAAA GATTCACGGA 2761 TTGGTTAACA GAGTCAACGC GAAGAATCTT GTTCAAATTG TCAGAGAACT TCTTCAAGAG 2821 AATGTGATTC GTTCAAAgtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt 2881 tttcagGGGA CTTCTCTGCC GTGACATTAT TCAAGCTCAG GCTTTCTCAC CAGGATTCTC 2941 TAACGTCTAT GCAGCTTTGG CGGCAGTTAT CAACTCGAAA TTCCCTCATG TCGGTGAACT 3001 TCTTCTCCGT CGTCTGATTG TACAGTTCAA AAGAAGTTTC CGTAGAAATG ACAGAGGCGT 3061 CACGGTGAAC GTGATCAAAT TCATCGCACA TTTGATTAAT CAACAAGTTG CTCACGAAGT 3121 TCTTGCGCTG GAAATCATGA TTCTGATGCT TGAAGAACCA ACTGATGATT CAGTTGAAGT 3181 CGCCATTGCG TTCCTGAAAG AGTGTGGAGC AAAGCTTCTG GAGATTGCTC CAGCAGCTCT 3241 TAACAGTGTC TACGACCGTC TTCGTGCAAT TCTCATGGAA ACTGAAAGAT CGGAAAATGC 3301 ACTGGATCGA CGTATTCAGT ATATGATTGA GACTGCAATG CAGATTCGAA AGGACAAATT 3361 TGCGGCGTAT CCGGCGGTCA TTGAAGACTT GGACTTGATT GAGGAGGAGG ATCAGATCAT 3421 CCATACACTT AATTTGGAGG ATGCGGTTGA TCCGGAAAAT GGGCTTAATG TGTTCAAACT 3481 AGATCCAGAA TTCGAAAAGA ACGAGGAGGT TTATGAGGAG ATCCGTAAGG AAATCATTGG 3541 AAACGCCGAT ATTTCGGATG AGGATGGTGG CGACGAGTTG GATGATGAAG AAGAGGGTAG 3601 TGATGTGGAA GAGGCTCCGA AGAAGACTAC AGAGATTATT GATAATACTG ATCAGAATTG 3661 ACTGCTTTCA GAAGgtattc attttgagtt ttgggccggc aaatctgtaa gttgccggtt 3721 gccgaaaatt tgctgaattt gccggaaaaa aaaattccgg aatttattta aaaacttttt 3781 gtaaaaatta aattaaattt gcaacttttc agAGAAGTCT ACCTGACAAT GCAATCATCT 3841 TTGGACTACC AAGAAGCTGC TCACAAATTG CTGAAAATGA AGATTCCAGA CAGCATGCAG 3901 gtcagcgatg ttgcaaagaa aaattttcga ccaaaaaaac caaccaatca taaaatttaa 3961 aaaaaaactc cgtttttttc ttttttttta tacgagaaaa accaaaaaaa tgtatttttg 4021 ccaaattcta aaatactatc cccgaaattt tcaatatttt ctctttcagA ACGAACTCTG 4081 CGCGATGCTT GTCGATTGTT GTGCTCAACA GCGTACCTAC GAGCGATTCT ACGGAATGCT 4141 CATCGAACGT TTCTGCCGAC TTCGCCTCGA ATACCAGCAA TACTTTGAAA AGCTCTGCCA 4201 GGACACGTAT TCCACGATTC ACCGAATTGA CATCACAAAA CTGCGGAATT TGGCTCGCCT 4261 TATTGCTCAT TTGCTCTCGA CGGATGCTAT TGACTGGAAG ATTTTGGCCG ATATGAAAAT 4321 GACCGAAGAG GACACAACTT CTTCTGGCAG AATCTATATT AAATATATAT TTAATGAACT 4381 TGTGGAGGCG ATGGGAATGG TTAAACTTCA TTCGAGAGTT ACTGATCCgt gagtttccta 4441 gagagagttg ttttcgtatt caattttccc tattttcagA ACTTTGGCTC ATTGCTTTGT 4501 TGGATTATTC CCACGAACTA ATCCGAACAG CGCACGATTT TCGATCAACT TCTTCACAAT 4561 GATTGGATTG GGTGGTTTGA CGTTGGAACT TCGTGAATGG CTGGCAAAGG GTCTCAAGAA 4621 GAAGAAGGGA ATGCTGGATC AGTTGAAGGC CGAATCAAGC TCAGATTCAT CGTCGTCTTC 4681 GGATTCGTCA GACTCGTCTG ATTCTTCGGA TTCTGACGAT TCATCCGACT CGTCTTCAGA 4741 TTCCTCATCT TCTTCAGAAT CAGAGCCAGA ACCACCGAAG AAAAAGAAGA AGAAGAACAG 4801 TGAAGAGAGT TCCAAAAAGA AGGAAAAAGA GAATATTGGT CGACGGGATC GTGGAGACAA 4861 GAGAGCTGAA CGTCATCGTG ATCAAAGTGT GGAGAACAAG GACAAGGATC GTCGACGTCG 4921 CCAGGATTCT GACGAAAATC GTCGGCCAGA ACGAGGAGAT GACCGCAAGG ATCGGAGTAA 4981 AGATCGTCGT CGTCAAGACT CGGATGATGA GGATCGGAAA GGTCGTGAAC GTCGGGAAGA 5041 TTCAGGGGAA AGACGTCGCG GAGATCGGGA TCGACGTGAT CGAAACAAGG ATCAGGAGGA 5101 TCACCGTGAA GATCGCCGTG ACCGAAGCAA GGATCGTGAG GATCGACGTG ATCGCCGTCG 5161 TCATGACTCT GATGATGATC GTAAAACTCG TCGGGATAGA AGTGAAGAGC GAGGAGGACG 5221 TCGTCGTGAA GTGGAATCGG ATGATCGACG CCGACGTCGT TGAATTTTCA AATTTTAAAT 5281 ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT 5341 TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt ttctctttcc gtctcccaat 5401 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca 5461 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa 5521 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa 5581 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat 5641 taggcgaacg atgagaattg tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt 5701 ttggcgaagg caaatgttcg gtatggagat ctgtaaaaat ttttaagttg aaatttggtg 5761 ttgctctttt acaaaatttt ccgattttcg cttgaaatta cggtgccagg tctcgacacg 5821 tcttccaatt tttcaaattc aaaagagcct ttaatgggct gtagttgcta atttctcgtt 5881 tttgaaaatt tttcttccgt ttaatcgaaa tttgatgtat tttatttatg attttcaata 5941 aatttcaaag aaactggtga aaactcggaa aattgtgaac tacagtaatc caatccttaa 6001 aggcgcacac cttttaaatg tccgccccaa tacgatattt ttttaagatt cgCTAGAGCG 6061 GCCCAAAAGG CCTTTTTTTT TTGGCGCCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 6121 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 6181 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 6241 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 6301 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 6361 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 6421 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 6481 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 6541 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 6601 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 6661 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 6721 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 6781 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 6841 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 6901 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 6961 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 7021 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 7081 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 7141 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 7201 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 7261 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 7321 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 7381 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 7441 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 7501 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 7561 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 7621 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 7681 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 7741 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 7801 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 7861 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 7921 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 7981 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 8041 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 8101 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 8161 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 8221 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat 8281 cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA 8341 CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1598 LOCUS ADDGENE_1598 9875 bp DNA circular SYN 01/03/2005 DEFINITION L3808 complete plasmid sequence KEYWORDS . SOURCE L3808 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1598 at www.addgene.org FEATURES Location/Qualifiers source 1..9875 /organism="Addgene plasmid L3808" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2440..2458) /label="T7_promoter" gene 2475..3323 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2475..3383 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 2646..2696 /label="SynIVS.R" misc_feature 2718..2737 /label="gfp.S65C" misc_feature 2851..2901 /label="SynIVS.S" misc_feature 3066..3116 /label="SynIVS.T" misc_feature 3381..3434 /label="let858.smgUTR" CDS 6059..6763 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 6739..6763 /label="let858.C" misc_feature 6841..6870 /label="let858.3'" promoter 7655..7683 /label="Amp_promoter" CDS 7725..8585 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 7725..8585 /label="Ampicillin" /gene="Ampicillin" rep_origin 8740..9359 /label="pBR322_origin" misc_feature 9575..9601 /label="lacI.C" promoter 9668..9697 /label="lac_promoter" misc_feature 9732..9750 /label="M13r_primer" misc_feature 9764..9814 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 2461 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 2521 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 2581 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 2641 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 2701 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA 2761 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 2821 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 2881 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 2941 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 3001 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 3061 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 3121 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 3181 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 3241 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 3301 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 3361 TCGCTAGCCG GCCATACAAG TAATccgggt tctagaagcg ctgctaaggg ggcccTCGTC 3421 GAGTCGGTCA CAATCACCTG AAACTCCAAA GGCAGCCAGT GAGGAACGTG AAGAAGAAGA 3481 AAAAGAGTCA TCTGAACAGg tttgattttc tttctggtca aaaagatgaa attattgatt 3541 ttcagCCAGA TACTCCCAAA ACTAGCAGCG AGAAGTCTGC AAGTCGTTCA CAGTCGCCCA 3601 GAGAATCGCG GGAAGTGAGC CAAGAGgtat gtttttcaaa aatcaataac tgatcataat 3661 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat 3721 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat 3781 ttcagACTGA AACTTCGGAA AATCAAGAGA AAATCAAAGA AAAGGATGAC GGGGATGATC 3841 AGCCTGGCAC ACCGAACAGC TATAGAAGCC GGGAAACTTC ACCAGCTCCA AAAAGGTCCA 3901 AGGAGACCAG gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagAGAA 3961 TCAGAGTCTC CTGAAAAATC CCCGGTTCGT TCAAGATCTC CCAGAAGGTC TTCAGCACGT 4021 TCCCCGTCAC GATCTCCTAG ACGGCGCCGA GAAAGAAGCT CAGAAAGAAA GCAATCCGAA 4081 GAGCCAGCAC CGCTACCAGA GAAAAAGAAG AAAGAGCCGC TGGATATTCT ACGAACAAGA 4141 ACCGGAGGAG CATATATTCC ACCCGCCAAA CTTCGACTTA TGCAACAACA GATTAGTGAT 4201 AAGCAAAGTG AACAGTATCA GAGAATGAAT TGGGAAAGAA TGAAGAAAAA GATTCACGGA 4261 TTGGTTAACA GAGTCAACGC GAAGAATCTT GTTCAAATTG TCAGAGAACT TCTTCAAGAG 4321 AATGTGATTC GTTCAAAgtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt 4381 tttcagGGGA CTTCTCTGCC GTGACATTAT TCAAGCTCAG GCTTTCTCAC CAGGATTCTC 4441 TAACGTCTAT GCAGCTTTGG CGGCAGTTAT CAACTCGAAA TTCCCTCATG TCGGTGAACT 4501 TCTTCTCCGT CGTCTGATTG TACAGTTCAA AAGAAGTTTC CGTAGAAATG ACAGAGGCGT 4561 CACGGTGAAC GTGATCAAAT TCATCGCACA TTTGATTAAT CAACAAGTTG CTCACGAAGT 4621 TCTTGCGCTG GAAATCATGA TTCTGATGCT TGAAGAACCA ACTGATGATT CAGTTGAAGT 4681 CGCCATTGCG TTCCTGAAAG AGTGTGGAGC AAAGCTTCTG GAGATTGCTC CAGCAGCTCT 4741 TAACAGTGTC TACGACCGTC TTCGTGCAAT TCTCATGGAA ACTGAAAGAT CGGAAAATGC 4801 ACTGGATCGA CGTATTCAGT ATATGATTGA GACTGCAATG CAGATTCGAA AGGACAAATT 4861 TGCGGCGTAT CCGGCGGTCA TTGAAGACTT GGACTTGATT GAGGAGGAGG ATCAGATCAT 4921 CCATACACTT AATTTGGAGG ATGCGGTTGA TCCGGAAAAT GGGCTTAATG TGTTCAAACT 4981 AGATCCAGAA TTCGAAAAGA ACGAGGAGGT TTATGAGGAG ATCCGTAAGG AAATCATTGG 5041 AAACGCCGAT ATTTCGGATG AGGATGGTGG CGACGAGTTG GATGATGAAG AAGAGGGTAG 5101 TGATGTGGAA GAGGCTCCGA AGAAGACTAC AGAGATTATT GATAATACTG ATCAGAATTG 5161 ACTGCTTTCA GAAGgtattc attttgagtt ttgggccggc aaatctgtaa gttgccggtt 5221 gccgaaaatt tgctgaattt gccggaaaaa aaaattccgg aatttattta aaaacttttt 5281 gtaaaaatta aattaaattt gcaacttttc agAGAAGTCT ACCTGACAAT GCAATCATCT 5341 TTGGACTACC AAGAAGCTGC TCACAAATTG CTGAAAATGA AGATTCCAGA CAGCATGCAG 5401 gtcagcgatg ttgcaaagaa aaattttcga ccaaaaaaac caaccaatca taaaatttaa 5461 aaaaaaactc cgtttttttc ttttttttta tacgagaaaa accaaaaaaa tgtatttttg 5521 ccaaattcta aaatactatc cccgaaattt tcaatatttt ctctttcagA ACGAACTCTG 5581 CGCGATGCTT GTCGATTGTT GTGCTCAACA GCGTACCTAC GAGCGATTCT ACGGAATGCT 5641 CATCGAACGT TTCTGCCGAC TTCGCCTCGA ATACCAGCAA TACTTTGAAA AGCTCTGCCA 5701 GGACACGTAT TCCACGATTC ACCGAATTGA CATCACAAAA CTGCGGAATT TGGCTCGCCT 5761 TATTGCTCAT TTGCTCTCGA CGGATGCTAT TGACTGGAAG ATTTTGGCCG ATATGAAAAT 5821 GACCGAAGAG GACACAACTT CTTCTGGCAG AATCTATATT AAATATATAT TTAATGAACT 5881 TGTGGAGGCG ATGGGAATGG TTAAACTTCA TTCGAGAGTT ACTGATCCgt gagtttccta 5941 gagagagttg ttttcgtatt caattttccc tattttcagA ACTTTGGCTC ATTGCTTTGT 6001 TGGATTATTC CCACGAACTA ATCCGAACAG CGCACGATTT TCGATCAACT TCTTCACAAT 6061 GATTGGATTG GGTGGTTTGA CGTTGGAACT TCGTGAATGG CTGGCAAAGG GTCTCAAGAA 6121 GAAGAAGGGA ATGCTGGATC AGTTGAAGGC CGAATCAAGC TCAGATTCAT CGTCGTCTTC 6181 GGATTCGTCA GACTCGTCTG ATTCTTCGGA TTCTGACGAT TCATCCGACT CGTCTTCAGA 6241 TTCCTCATCT TCTTCAGAAT CAGAGCCAGA ACCACCGAAG AAAAAGAAGA AGAAGAACAG 6301 TGAAGAGAGT TCCAAAAAGA AGGAAAAAGA GAATATTGGT CGACGGGATC GTGGAGACAA 6361 GAGAGCTGAA CGTCATCGTG ATCAAAGTGT GGAGAACAAG GACAAGGATC GTCGACGTCG 6421 CCAGGATTCT GACGAAAATC GTCGGCCAGA ACGAGGAGAT GACCGCAAGG ATCGGAGTAA 6481 AGATCGTCGT CGTCAAGACT CGGATGATGA GGATCGGAAA GGTCGTGAAC GTCGGGAAGA 6541 TTCAGGGGAA AGACGTCGCG GAGATCGGGA TCGACGTGAT CGAAACAAGG ATCAGGAGGA 6601 TCACCGTGAA GATCGCCGTG ACCGAAGCAA GGATCGTGAG GATCGACGTG ATCGCCGTCG 6661 TCATGACTCT GATGATGATC GTAAAACTCG TCGGGATAGA AGTGAAGAGC GAGGAGGACG 6721 TCGTCGTGAA GTGGAATCGG ATGATCGACG CCGACGTCGT TGAATTTTCA AATTTTAAAT 6781 ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT 6841 TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt ttctctttcc gtctcccaat 6901 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca 6961 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa 7021 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa 7081 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat 7141 taggcgaacg atgagaattg tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt 7201 ttggcgaagg caaatgttcg gtatggagat ctgtaaaaat ttttaagttg aaatttggtg 7261 ttgctctttt acaaaatttt ccgattttcg cttgaaatta cggtgccagg tctcgacacg 7321 tcttccaatt tttcaaattc aaaagagcct ttaatgggct gtagttgcta atttctcgtt 7381 tttgaaaatt tttcttccgt ttaatcgaaa tttgatgtat tttatttatg attttcaata 7441 aatttcaaag aaactggtga aaactcggaa aattgtgaac tacagtaatc caatccttaa 7501 aggcgcacac cttttaaatg tccgccccaa tacgatattt ttttaagatt cgCTAGAGCG 7561 GCCCAAAAGG CCTTTTTTTT TTGGCGCCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 7621 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 7681 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 7741 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 7801 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 7861 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 7921 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 7981 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 8041 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 8101 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 8161 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 8221 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 8281 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 8341 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 8401 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 8461 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 8521 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 8581 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 8641 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 8701 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 8761 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 8821 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 8881 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 8941 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 9001 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 9061 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 9121 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 9181 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 9241 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 9301 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 9361 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 9421 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 9481 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 9541 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 9601 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 9661 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 9721 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat 9781 cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA 9841 CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1599 LOCUS ADDGENE_1599 10970 bp DNA circular SYN 01/03/2005 DEFINITION L3809 complete plasmid sequence KEYWORDS . SOURCE L3809 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1599 at www.addgene.org FEATURES Location/Qualifiers source 1..10970 /organism="Addgene plasmid L3809" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" promoter complement(3535..3553) /label="T7_promoter" CDS 3570..4478 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 3570..4418 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3741..3791 /label="SynIVS.R" misc_feature 3813..3832 /label="gfp.S65C" misc_feature 3946..3996 /label="SynIVS.S" misc_feature 4161..4211 /label="SynIVS.T" misc_feature 4476..4529 /label="let858.smgUTR" CDS 7154..7858 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 7834..7858 /label="let858.C" misc_feature 7936..7965 /label="let858.3'" promoter 8750..8778 /label="Amp_promoter" CDS 8820..9680 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8820..9680 /label="Ampicillin" /gene="Ampicillin" rep_origin 9835..10454 /label="pBR322_origin" misc_feature 10670..10696 /label="lacI.C" promoter 10763..10792 /label="lac_promoter" misc_feature 10827..10845 /label="M13r_primer" misc_feature 10859..10909 /label="decoy" misc_feature 10967..10990 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtggtacc gctagccgta 3481 cgaacccggg ttCTAGAGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 3541 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 3601 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 3661 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 3721 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 3781 taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCGAGAT 3841 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 3901 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 3961 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 4021 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 4081 TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC 4141 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 4201 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 4261 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 4321 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 4381 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGAcGAACTA TACAAAagcc 4441 cacaAGCTTT GGAATTCGCT AGCCGGCCAT ACAAGTAATc cgggttctag aagcgctgct 4501 aagggggccc TCGTCGAGTC GGTCACAATC ACCTGAAACT CCAAAGGCAG CCAGTGAGGA 4561 ACGTGAAGAA GAAGAAAAAG AGTCATCTGA ACAGgtttga ttttctttct ggtcaaaaag 4621 atgaaattat tgattttcag CCAGATACTC CCAAAACTAG CAGCGAGAAG TCTGCAAGTC 4681 GTTCACAGTC GCCCAGAGAA TCGCGGGAAG TGAGCCAAGA Ggtatgtttt tcaaaaatca 4741 ataactgatc ataattttta ttgtttggtg aatttaagaa aataatattc gaaaattcct 4801 ctgaattatc aagattgcag tattaatttc gagaaaaatt gagatattca tagagctatt 4861 gtaaattttc ttgatttcag ACTGAAACTT CGGAAAATCA AGAGAAAATC AAAGAAAAGG 4921 ATGACGGGGA TGATCAGCCT GGCACACCGA ACAGCTATAG AAGCCGGGAA ACTTCACCAG 4981 CTCCAAAAAG GTCCAAGGAG ACCAGgtttg tcaaaagctt cctgcgatta attctcattt 5041 caatttttca gAGAATCAGA GTCTCCTGAA AAATCCCCGG TTCGTTCAAG ATCTCCCAGA 5101 AGGTCTTCAG CACGTTCCCC GTCACGATCT CCTAGACGGC GCCGAGAAAG AAGCTCAGAA 5161 AGAAAGCAAT CCGAAGAGCC AGCACCGCTA CCAGAGAAAA AGAAGAAAGA GCCGCTGGAT 5221 ATTCTACGAA CAAGAACCGG AGGAGCATAT ATTCCACCCG CCAAACTTCG ACTTATGCAA 5281 CAACAGATTA GTGATAAGCA AAGTGAACAG TATCAGAGAA TGAATTGGGA AAGAATGAAG 5341 AAAAAGATTC ACGGATTGGT TAACAGAGTC AACGCGAAGA ATCTTGTTCA AATTGTCAGA 5401 GAACTTCTTC AAGAGAATGT GATTCGTTCA AAgtgagtga gaaaatcgaa ggaaaaggaa 5461 agaattaatt taatttttca gGGGACTTCT CTGCCGTGAC ATTATTCAAG CTCAGGCTTT 5521 CTCACCAGGA TTCTCTAACG TCTATGCAGC TTTGGCGGCA GTTATCAACT CGAAATTCCC 5581 TCATGTCGGT GAACTTCTTC TCCGTCGTCT GATTGTACAG TTCAAAAGAA GTTTCCGTAG 5641 AAATGACAGA GGCGTCACGG TGAACGTGAT CAAATTCATC GCACATTTGA TTAATCAACA 5701 AGTTGCTCAC GAAGTTCTTG CGCTGGAAAT CATGATTCTG ATGCTTGAAG AACCAACTGA 5761 TGATTCAGTT GAAGTCGCCA TTGCGTTCCT GAAAGAGTGT GGAGCAAAGC TTCTGGAGAT 5821 TGCTCCAGCA GCTCTTAACA GTGTCTACGA CCGTCTTCGT GCAATTCTCA TGGAAACTGA 5881 AAGATCGGAA AATGCACTGG ATCGACGTAT TCAGTATATG ATTGAGACTG CAATGCAGAT 5941 TCGAAAGGAC AAATTTGCGG CGTATCCGGC GGTCATTGAA GACTTGGACT TGATTGAGGA 6001 GGAGGATCAG ATCATCCATA CACTTAATTT GGAGGATGCG GTTGATCCGG AAAATGGGCT 6061 TAATGTGTTC AAACTAGATC CAGAATTCGA AAAGAACGAG GAGGTTTATG AGGAGATCCG 6121 TAAGGAAATC ATTGGAAACG CCGATATTTC GGATGAGGAT GGTGGCGACG AGTTGGATGA 6181 TGAAGAAGAG GGTAGTGATG TGGAAGAGGC TCCGAAGAAG ACTACAGAGA TTATTGATAA 6241 TACTGATCAG AATTGACTGC TTTCAGAAGg tattcatttt gagttttggg ccggcaaatc 6301 tgtaagttgc cggttgccga aaatttgctg aatttgccgg aaaaaaaaat tccggaattt 6361 atttaaaaac tttttgtaaa aattaaatta aatttgcaac ttttcagAGA AGTCTACCTG 6421 ACAATGCAAT CATCTTTGGA CTACCAAGAA GCTGCTCACA AATTGCTGAA AATGAAGATT 6481 CCAGACAGCA TGCAGgtcag cgatgttgca aagaaaaatt ttcgaccaaa aaaaccaacc 6541 aatcataaaa tttaaaaaaa aactccgttt ttttcttttt ttttatacga gaaaaaccaa 6601 aaaaatgtat ttttgccaaa ttctaaaata ctatccccga aattttcaat attttctctt 6661 tcagAACGAA CTCTGCGCGA TGCTTGTCGA TTGTTGTGCT CAACAGCGTA CCTACGAGCG 6721 ATTCTACGGA ATGCTCATCG AACGTTTCTG CCGACTTCGC CTCGAATACC AGCAATACTT 6781 TGAAAAGCTC TGCCAGGACA CGTATTCCAC GATTCACCGA ATTGACATCA CAAAACTGCG 6841 GAATTTGGCT CGCCTTATTG CTCATTTGCT CTCGACGGAT GCTATTGACT GGAAGATTTT 6901 GGCCGATATG AAAATGACCG AAGAGGACAC AACTTCTTCT GGCAGAATCT ATATTAAATA 6961 TATATTTAAT GAACTTGTGG AGGCGATGGG AATGGTTAAA CTTCATTCGA GAGTTACTGA 7021 TCCgtgagtt tcctagagag agttgttttc gtattcaatt ttccctattt tcagAACTTT 7081 GGCTCATTGC TTTGTTGGAT TATTCCCACG AACTAATCCG AACAGCGCAC GATTTTCGAT 7141 CAACTTCTTC ACAATGATTG GATTGGGTGG TTTGACGTTG GAACTTCGTG AATGGCTGGC 7201 AAAGGGTCTC AAGAAGAAGA AGGGAATGCT GGATCAGTTG AAGGCCGAAT CAAGCTCAGA 7261 TTCATCGTCG TCTTCGGATT CGTCAGACTC GTCTGATTCT TCGGATTCTG ACGATTCATC 7321 CGACTCGTCT TCAGATTCCT CATCTTCTTC AGAATCAGAG CCAGAACCAC CGAAGAAAAA 7381 GAAGAAGAAG AACAGTGAAG AGAGTTCCAA AAAGAAGGAA AAAGAGAATA TTGGTCGACG 7441 GGATCGTGGA GACAAGAGAG CTGAACGTCA TCGTGATCAA AGTGTGGAGA ACAAGGACAA 7501 GGATCGTCGA CGTCGCCAGG ATTCTGACGA AAATCGTCGG CCAGAACGAG GAGATGACCG 7561 CAAGGATCGG AGTAAAGATC GTCGTCGTCA AGACTCGGAT GATGAGGATC GGAAAGGTCG 7621 TGAACGTCGG GAAGATTCAG GGGAAAGACG TCGCGGAGAT CGGGATCGAC GTGATCGAAA 7681 CAAGGATCAG GAGGATCACC GTGAAGATCG CCGTGACCGA AGCAAGGATC GTGAGGATCG 7741 ACGTGATCGC CGTCGTCATG ACTCTGATGA TGATCGTAAA ACTCGTCGGG ATAGAAGTGA 7801 AGAGCGAGGA GGACGTCGTC GTGAAGTGGA ATCGGATGAT CGACGCCGAC GTCGTTGAAT 7861 TTTCAAATTT TAAATACTGA ATATTTGTTT TTTTTCCTAT TATTTATTTA TTCTCTTTGT 7921 GTTTTTTTTC TTGCTTTCTA AAAAATTAAT TCAATCCAAA TCTAAacatt tttttttctc 7981 tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt 8041 atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca 8101 aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata 8161 tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa actagtaaaa 8221 aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttggcaaa tgcgaatccg 8281 tatggagagg cacgtttggc gaaggcaaat gttcggtatg gagatctgta aaaattttta 8341 agttgaaatt tggtgttgct cttttacaaa attttccgat tttcgcttga aattacggtg 8401 ccaggtctcg acacgtcttc caatttttca aattcaaaag agcctttaat gggctgtagt 8461 tgctaatttc tcgtttttga aaatttttct tccgtttaat cgaaatttga tgtattttat 8521 ttatgatttt caataaattt caaagaaact ggtgaaaact cggaaaattg tgaactacag 8581 taatccaatc cttaaaggcg cacacctttt aaatgtccgc cccaatacga tattttttta 8641 agattcgCTA GAGCGGCCCA AAAGGCCTTT TTTTTTTGGC GCCGACGTCA GGTGGCACTT 8701 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 8761 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 8821 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 8881 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 8941 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 9001 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 9061 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 9121 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 9181 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 9241 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 9301 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 9361 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 9421 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 9481 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 9541 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 9601 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 9661 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 9721 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 9781 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 9841 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 9901 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 9961 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 10021 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 10081 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 10141 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 10201 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 10261 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 10321 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 10381 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 10441 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 10501 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 10561 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 10621 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 10681 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 10741 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 10801 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 10861 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 10921 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1600 LOCUS ADDGENE_1600 8775 bp DNA circular SYN 01/03/2005 DEFINITION L3811 complete plasmid sequence KEYWORDS . SOURCE L3811 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1600 at www.addgene.org FEATURES Location/Qualifiers source 1..8775 /organism="Addgene plasmid L3811" /mol_type="other DNA" misc_feature 20..44 /label="myo2.-1090" misc_feature 1108..1131 /label="myo2.5'" misc_feature 1205..1246 /label="SynIVS.A" misc_feature 1253..1270 /label="rf21.28" promoter complement(1340..1358) /label="T7_promoter" gene 1375..2223 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1375..2283 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 1546..1596 /label="SynIVS.R" misc_feature 1618..1637 /label="gfp.S65C" misc_feature 1751..1801 /label="SynIVS.S" misc_feature 1966..2016 /label="SynIVS.T" misc_feature 2281..2334 /label="let858.smgUTR" CDS 4959..5663 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 5639..5663 /label="let858.C" misc_feature 5741..5770 /label="let858.3'" promoter 6555..6583 /label="Amp_promoter" CDS 6625..7485 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6625..7485 /label="Ampicillin" /gene="Ampicillin" rep_origin 7640..8259 /label="pBR322_origin" misc_feature 8475..8501 /label="lacI.C" promoter 8568..8597 /label="lac_promoter" misc_feature 8632..8650 /label="M13r_primer" misc_feature 8664..8714 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGAGGCATTT GAATTGGGGG TGGTGGACAG TAACTGTCTG 61 TAATAATAAT TACTCCTGAC CAGGTTGCAA TTCGAGTTTT GATAAGCATA ATTATACCTT 121 GTACATTGTG GGTTTTGTGC TGTGGACGTT TTATTGTGAC ATCCCCATAA GCTACAAGAA 181 CAAAAATGAA ATgTAAAAGT ATTGAAAACG TCGTACATTT TATATCTGAG TAGTATCCTT 241 TGCTTTAAAT GTCCATAAAA cTAATTTTAT AATCAATAAA ACAACGTTTG TAAATCAACT 301 GAGTTTACAA GTAGAGACAT TGAGGGATAC TTTCACTATG CTAAAGTGAT AATCGACCAA 361 ATAATAACTt CACTTTGGTA TTTATTCCTG TCTTATAAaT GTTATGTATG AATTAAATTC 421 ATATGCATAT GGCTCACTCT GACAAttttt AATAATCTTC CAGATCAATA TTGACTACCG 481 ATGCGGGTGG TCTTTTGCTT TGAATTCTGC TGAACTTTAC ACCCCGAACA GCAATGTGTG 541 CTTCAGCcTA AAAAAAAGTA AGTGTGTTAA TCAGTgCCCC CGATTCTTCA TTTTTTGCCC 601 CTCTCTCCCG TTTCGTCGGC AAAAGAAGAG AAAATAAAGA TAAGTCTCAA GATAGGTTGG 661 TAATCGCTAA AGTGGTTGTG TGGATAAGAG TAGCAAAATG GCAGGAAGAG CACTTTGCGC 721 GCACACACTG TACTCATTGT TCTGGATAAA ATTCTCTCGT TGTTTGCCGT CGGATGTCTG 781 CCTCTCTGCA TTGAGCCGGC TTCTTCACTA TCTTTAGTTA ACCTAAAATG CCGTTTCTTT 841 TCTCGTATCC CcACTATcCC GTTGAGGTTC TCTGCTCTCT TCGCTCCCTA CCGCCAGCGA 901 GCAACTATCC GTGGGGGCGC CTTGCTCGGA AGATGGGGGG GAAGAAAGAA GATTTTTGCT 961 ATTTGCACTT GAGAAAGAGA CTTTTCCTGC GTCGATGGTT AGAGAACAGT GTGCAGACAC 1021 TTTTCAGCTA CCTAGAatTA CAatTGGATA TCCCCGCCTC CCAATCCACC CACCCAGGGA 1081 AAAAGAAGGG CTCGCCGAAA ATCAAAGTTA TCTCCAGGCT CGCGCATCCC ACCGAGCGGT 1141 TGACTTCTCT CCACCACTTT TCATTTTAAC CCTCGGGGTA CGGGATTGGC CAAAGGACCC 1201 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGCT 1261 AGCGTCGACG GTACCGGTCA GATATCACTC GAGGAGCTCC CGAGATCCTA TCGATTCGCG 1321 GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG TACCGGTAGA AAAAATGAGT 1381 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT 1441 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT 1501 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag tttaaacata 1561 tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC TACTTTCTgT 1621 TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA AACAGCATGA CTTTTTCAAG 1681 AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA TGACGGGAAC 1741 TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt taaattttca 1801 gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT AAAAGGTATT 1861 GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA TAACTCACAC 1921 AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag tttaaacatg 1981 attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG ACACAACATT 2041 GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT TGGCGATGGC 2101 CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC 2161 AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG GATTACACAT 2221 GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT TCGCTAGCCG GCCATACAAG 2281 TAATccgggt tctagaagcg ctgctaaggg ggcccTCGTC GAGTCGGTCA CAATCACCTG 2341 AAACTCCAAA GGCAGCCAGT GAGGAACGTG AAGAAGAAGA AAAAGAGTCA TCTGAACAGg 2401 tttgattttc tttctggtca aaaagatgaa attattgatt ttcagCCAGA TACTCCCAAA 2461 ACTAGCAGCG AGAAGTCTGC AAGTCGTTCA CAGTCGCCCA GAGAATCGCG GGAAGTGAGC 2521 CAAGAGgtat gtttttcaaa aatcaataac tgatcataat ttttattgtt tggtgaattt 2581 aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat tgcagtatta atttcgagaa 2641 aaattgagat attcatagag ctattgtaaa ttttcttgat ttcagACTGA AACTTCGGAA 2701 AATCAAGAGA AAATCAAAGA AAAGGATGAC GGGGATGATC AGCCTGGCAC ACCGAACAGC 2761 TATAGAAGCC GGGAAACTTC ACCAGCTCCA AAAAGGTCCA AGGAGACCAG gtttgtcaaa 2821 agcttcctgc gattaattct catttcaatt tttcagAGAA TCAGAGTCTC CTGAAAAATC 2881 CCCGGTTCGT TCAAGATCTC CCAGAAGGTC TTCAGCACGT TCCCCGTCAC GATCTCCTAG 2941 ACGGCGCCGA GAAAGAAGCT CAGAAAGAAA GCAATCCGAA GAGCCAGCAC CGCTACCAGA 3001 GAAAAAGAAG AAAGAGCCGC TGGATATTCT ACGAACAAGA ACCGGAGGAG CATATATTCC 3061 ACCCGCCAAA CTTCGACTTA TGCAACAACA GATTAGTGAT AAGCAAAGTG AACAGTATCA 3121 GAGAATGAAT TGGGAAAGAA TGAAGAAAAA GATTCACGGA TTGGTTAACA GAGTCAACGC 3181 GAAGAATCTT GTTCAAATTG TCAGAGAACT TCTTCAAGAG AATGTGATTC GTTCAAAgtg 3241 agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt tttcagGGGA CTTCTCTGCC 3301 GTGACATTAT TCAAGCTCAG GCTTTCTCAC CAGGATTCTC TAACGTCTAT GCAGCTTTGG 3361 CGGCAGTTAT CAACTCGAAA TTCCCTCATG TCGGTGAACT TCTTCTCCGT CGTCTGATTG 3421 TACAGTTCAA AAGAAGTTTC CGTAGAAATG ACAGAGGCGT CACGGTGAAC GTGATCAAAT 3481 TCATCGCACA TTTGATTAAT CAACAAGTTG CTCACGAAGT TCTTGCGCTG GAAATCATGA 3541 TTCTGATGCT TGAAGAACCA ACTGATGATT CAGTTGAAGT CGCCATTGCG TTCCTGAAAG 3601 AGTGTGGAGC AAAGCTTCTG GAGATTGCTC CAGCAGCTCT TAACAGTGTC TACGACCGTC 3661 TTCGTGCAAT TCTCATGGAA ACTGAAAGAT CGGAAAATGC ACTGGATCGA CGTATTCAGT 3721 ATATGATTGA GACTGCAATG CAGATTCGAA AGGACAAATT TGCGGCGTAT CCGGCGGTCA 3781 TTGAAGACTT GGACTTGATT GAGGAGGAGG ATCAGATCAT CCATACACTT AATTTGGAGG 3841 ATGCGGTTGA TCCGGAAAAT GGGCTTAATG TGTTCAAACT AGATCCAGAA TTCGAAAAGA 3901 ACGAGGAGGT TTATGAGGAG ATCCGTAAGG AAATCATTGG AAACGCCGAT ATTTCGGATG 3961 AGGATGGTGG CGACGAGTTG GATGATGAAG AAGAGGGTAG TGATGTGGAA GAGGCTCCGA 4021 AGAAGACTAC AGAGATTATT GATAATACTG ATCAGAATTG ACTGCTTTCA GAAGgtattc 4081 attttgagtt ttgggccggc aaatctgtaa gttgccggtt gccgaaaatt tgctgaattt 4141 gccggaaaaa aaaattccgg aatttattta aaaacttttt gtaaaaatta aattaaattt 4201 gcaacttttc agAGAAGTCT ACCTGACAAT GCAATCATCT TTGGACTACC AAGAAGCTGC 4261 TCACAAATTG CTGAAAATGA AGATTCCAGA CAGCATGCAG gtcagcgatg ttgcaaagaa 4321 aaattttcga ccaaaaaaac caaccaatca taaaatttaa aaaaaaactc cgtttttttc 4381 ttttttttta tacgagaaaa accaaaaaaa tgtatttttg ccaaattcta aaatactatc 4441 cccgaaattt tcaatatttt ctctttcagA ACGAACTCTG CGCGATGCTT GTCGATTGTT 4501 GTGCTCAACA GCGTACCTAC GAGCGATTCT ACGGAATGCT CATCGAACGT TTCTGCCGAC 4561 TTCGCCTCGA ATACCAGCAA TACTTTGAAA AGCTCTGCCA GGACACGTAT TCCACGATTC 4621 ACCGAATTGA CATCACAAAA CTGCGGAATT TGGCTCGCCT TATTGCTCAT TTGCTCTCGA 4681 CGGATGCTAT TGACTGGAAG ATTTTGGCCG ATATGAAAAT GACCGAAGAG GACACAACTT 4741 CTTCTGGCAG AATCTATATT AAATATATAT TTAATGAACT TGTGGAGGCG ATGGGAATGG 4801 TTAAACTTCA TTCGAGAGTT ACTGATCCgt gagtttccta gagagagttg ttttcgtatt 4861 caattttccc tattttcagA ACTTTGGCTC ATTGCTTTGT TGGATTATTC CCACGAACTA 4921 ATCCGAACAG CGCACGATTT TCGATCAACT TCTTCACAAT GATTGGATTG GGTGGTTTGA 4981 CGTTGGAACT TCGTGAATGG CTGGCAAAGG GTCTCAAGAA GAAGAAGGGA ATGCTGGATC 5041 AGTTGAAGGC CGAATCAAGC TCAGATTCAT CGTCGTCTTC GGATTCGTCA GACTCGTCTG 5101 ATTCTTCGGA TTCTGACGAT TCATCCGACT CGTCTTCAGA TTCCTCATCT TCTTCAGAAT 5161 CAGAGCCAGA ACCACCGAAG AAAAAGAAGA AGAAGAACAG TGAAGAGAGT TCCAAAAAGA 5221 AGGAAAAAGA GAATATTGGT CGACGGGATC GTGGAGACAA GAGAGCTGAA CGTCATCGTG 5281 ATCAAAGTGT GGAGAACAAG GACAAGGATC GTCGACGTCG CCAGGATTCT GACGAAAATC 5341 GTCGGCCAGA ACGAGGAGAT GACCGCAAGG ATCGGAGTAA AGATCGTCGT CGTCAAGACT 5401 CGGATGATGA GGATCGGAAA GGTCGTGAAC GTCGGGAAGA TTCAGGGGAA AGACGTCGCG 5461 GAGATCGGGA TCGACGTGAT CGAAACAAGG ATCAGGAGGA TCACCGTGAA GATCGCCGTG 5521 ACCGAAGCAA GGATCGTGAG GATCGACGTG ATCGCCGTCG TCATGACTCT GATGATGATC 5581 GTAAAACTCG TCGGGATAGA AGTGAAGAGC GAGGAGGACG TCGTCGTGAA GTGGAATCGG 5641 ATGATCGACG CCGACGTCGT TGAATTTTCA AATTTTAAAT ACTGAATATT TGTTTTTTTT 5701 CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT TTCTAAAAAA TTAATTCAAT 5761 CCAAATCTAA acattttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat 5821 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg 5881 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata 5941 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat 6001 agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgagaattg 6061 tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt ttggcgaagg caaatgttcg 6121 gtatggagat ctgtaaaaat ttttaagttg aaatttggtg ttgctctttt acaaaatttt 6181 ccgattttcg cttgaaatta cggtgccagg tctcgacacg tcttccaatt tttcaaattc 6241 aaaagagcct ttaatgggct gtagttgcta atttctcgtt tttgaaaatt tttcttccgt 6301 ttaatcgaaa tttgatgtat tttatttatg attttcaata aatttcaaag aaactggtga 6361 aaactcggaa aattgtgaac tacagtaatc caatccttaa aggcgcacac cttttaaatg 6421 tccgccccaa tacgatattt ttttaagatt cgCTAGAGCG GCCCAAAAGG CCTTTTTTTT 6481 TTGGCGCCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 6541 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 6601 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 6661 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 6721 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 6781 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 6841 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 6901 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 6961 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 7021 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 7081 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 7141 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 7201 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 7261 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 7321 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 7381 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 7441 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 7501 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 7561 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 7621 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 7681 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 7741 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 7801 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 7861 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 7921 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 7981 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 8041 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 8101 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 8161 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 8221 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 8281 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 8341 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 8401 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 8461 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 8521 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 8581 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 8641 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 8701 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 8761 ACTTGGAAAT GAAAT // 1601 LOCUS ADDGENE_1601 7518 bp DNA circular SYN 01/03/2005 DEFINITION L3812 complete plasmid sequence KEYWORDS . SOURCE L3812 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1601 at www.addgene.org FEATURES Location/Qualifiers source 1..7518 /organism="Addgene plasmid L3812" /mol_type="other DNA" promoter complement(83..101) /label="T7_promoter" gene 118..966 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 118..1026 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 289..339 /label="SynIVS.R" misc_feature 361..380 /label="gfp.S65C" misc_feature 494..544 /label="SynIVS.S" misc_feature 709..759 /label="SynIVS.T" misc_feature 1024..1077 /label="let858.smgUTR" CDS 3702..4406 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 4382..4406 /label="let858.C" misc_feature 4484..4513 /label="let858.3'" promoter 5298..5326 /label="Amp_promoter" CDS 5368..6228 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5368..6228 /label="Ampicillin" /gene="Ampicillin" rep_origin 6383..7002 /label="pBR322_origin" misc_feature 7218..7244 /label="lacI.C" promoter 7311..7340 /label="lac_promoter" misc_feature 7375..7393 /label="M13r_primer" misc_feature 7407..7457 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGGATC CTATCGATTC 61 GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT AGAAAAAATG 121 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 181 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 241 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac 301 atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC 361 TgTTATGGTG TTCAATGCTT cTCGAGATAC CCAGATCATA TGAAACAGCA TGACTTTTTC 421 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 481 AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt 541 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT 601 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA 661 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac 721 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC 781 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT 841 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT 901 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA 961 CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCGCTAG CCGGCCATAC 1021 AAGTAATccg ggttctagaa gcgctgctaa gggggcccTC GTCGAGTCGG TCACAATCAC 1081 CTGAAACTCC AAAGGCAGCC AGTGAGGAAC GTGAAGAAGA AGAAAAAGAG TCATCTGAAC 1141 AGgtttgatt ttctttctgg tcaaaaagat gaaattattg attttcagCC AGATACTCCC 1201 AAAACTAGCA GCGAGAAGTC TGCAAGTCGT TCACAGTCGC CCAGAGAATC GCGGGAAGTG 1261 AGCCAAGAGg tatgtttttc aaaaatcaat aactgatcat aatttttatt gtttggtgaa 1321 tttaagaaaa taatattcga aaattcctct gaattatcaa gattgcagta ttaatttcga 1381 gaaaaattga gatattcata gagctattgt aaattttctt gatttcagAC TGAAACTTCG 1441 GAAAATCAAG AGAAAATCAA AGAAAAGGAT GACGGGGATG ATCAGCCTGG CACACCGAAC 1501 AGCTATAGAA GCCGGGAAAC TTCACCAGCT CCAAAAAGGT CCAAGGAGAC CAGgtttgtc 1561 aaaagcttcc tgcgattaat tctcatttca atttttcagA GAATCAGAGT CTCCTGAAAA 1621 ATCCCCGGTT CGTTCAAGAT CTCCCAGAAG GTCTTCAGCA CGTTCCCCGT CACGATCTCC 1681 TAGACGGCGC CGAGAAAGAA GCTCAGAAAG AAAGCAATCC GAAGAGCCAG CACCGCTACC 1741 AGAGAAAAAG AAGAAAGAGC CGCTGGATAT TCTACGAACA AGAACCGGAG GAGCATATAT 1801 TCCACCCGCC AAACTTCGAC TTATGCAACA ACAGATTAGT GATAAGCAAA GTGAACAGTA 1861 TCAGAGAATG AATTGGGAAA GAATGAAGAA AAAGATTCAC GGATTGGTTA ACAGAGTCAA 1921 CGCGAAGAAT CTTGTTCAAA TTGTCAGAGA ACTTCTTCAA GAGAATGTGA TTCGTTCAAA 1981 gtgagtgaga aaatcgaagg aaaaggaaag aattaattta atttttcagG GGACTTCTCT 2041 GCCGTGACAT TATTCAAGCT CAGGCTTTCT CACCAGGATT CTCTAACGTC TATGCAGCTT 2101 TGGCGGCAGT TATCAACTCG AAATTCCCTC ATGTCGGTGA ACTTCTTCTC CGTCGTCTGA 2161 TTGTACAGTT CAAAAGAAGT TTCCGTAGAA ATGACAGAGG CGTCACGGTG AACGTGATCA 2221 AATTCATCGC ACATTTGATT AATCAACAAG TTGCTCACGA AGTTCTTGCG CTGGAAATCA 2281 TGATTCTGAT GCTTGAAGAA CCAACTGATG ATTCAGTTGA AGTCGCCATT GCGTTCCTGA 2341 AAGAGTGTGG AGCAAAGCTT CTGGAGATTG CTCCAGCAGC TCTTAACAGT GTCTACGACC 2401 GTCTTCGTGC AATTCTCATG GAAACTGAAA GATCGGAAAA TGCACTGGAT CGACGTATTC 2461 AGTATATGAT TGAGACTGCA ATGCAGATTC GAAAGGACAA ATTTGCGGCG TATCCGGCGG 2521 TCATTGAAGA CTTGGACTTG ATTGAGGAGG AGGATCAGAT CATCCATACA CTTAATTTGG 2581 AGGATGCGGT TGATCCGGAA AATGGGCTTA ATGTGTTCAA ACTAGATCCA GAATTCGAAA 2641 AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT TGGAAACGCC GATATTTCGG 2701 ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG TAGTGATGTG GAAGAGGCTC 2761 CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA TTGACTGCTT TCAGAAGgta 2821 ttcattttga gttttgggcc ggcaaatctg taagttgccg gttgccgaaa atttgctgaa 2881 tttgccggaa aaaaaaattc cggaatttat ttaaaaactt tttgtaaaaa ttaaattaaa 2941 tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA TCTTTGGACT ACCAAGAAGC 3001 TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG CAGgtcagcg atgttgcaaa 3061 gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt taaaaaaaaa ctccgttttt 3121 ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt ttgccaaatt ctaaaatact 3181 atccccgaaa ttttcaatat tttctctttc agAACGAACT CTGCGCGATG CTTGTCGATT 3241 GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGAAT GCTCATCGAA CGTTTCTGCC 3301 GACTTCGCCT CGAATACCAG CAATACTTTG AAAAGCTCTG CCAGGACACG TATTCCACGA 3361 TTCACCGAAT TGACATCACA AAACTGCGGA ATTTGGCTCG CCTTATTGCT CATTTGCTCT 3421 CGACGGATGC TATTGACTGG AAGATTTTGG CCGATATGAA AATGACCGAA GAGGACACAA 3481 CTTCTTCTGG CAGAATCTAT ATTAAATATA TATTTAATGA ACTTGTGGAG GCGATGGGAA 3541 TGGTTAAACT TCATTCGAGA GTTACTGATC Cgtgagtttc ctagagagag ttgttttcgt 3601 attcaatttt ccctattttc agAACTTTGG CTCATTGCTT TGTTGGATTA TTCCCACGAA 3661 CTAATCCGAA CAGCGCACGA TTTTCGATCA ACTTCTTCAC AATGATTGGA TTGGGTGGTT 3721 TGACGTTGGA ACTTCGTGAA TGGCTGGCAA AGGGTCTCAA GAAGAAGAAG GGAATGCTGG 3781 ATCAGTTGAA GGCCGAATCA AGCTCAGATT CATCGTCGTC TTCGGATTCG TCAGACTCGT 3841 CTGATTCTTC GGATTCTGAC GATTCATCCG ACTCGTCTTC AGATTCCTCA TCTTCTTCAG 3901 AATCAGAGCC AGAACCACCG AAGAAAAAGA AGAAGAAGAA CAGTGAAGAG AGTTCCAAAA 3961 AGAAGGAAAA AGAGAATATT GGTCGACGGG ATCGTGGAGA CAAGAGAGCT GAACGTCATC 4021 GTGATCAAAG TGTGGAGAAC AAGGACAAGG ATCGTCGACG TCGCCAGGAT TCTGACGAAA 4081 ATCGTCGGCC AGAACGAGGA GATGACCGCA AGGATCGGAG TAAAGATCGT CGTCGTCAAG 4141 ACTCGGATGA TGAGGATCGG AAAGGTCGTG AACGTCGGGA AGATTCAGGG GAAAGACGTC 4201 GCGGAGATCG GGATCGACGT GATCGAAACA AGGATCAGGA GGATCACCGT GAAGATCGCC 4261 GTGACCGAAG CAAGGATCGT GAGGATCGAC GTGATCGCCG TCGTCATGAC TCTGATGATG 4321 ATCGTAAAAC TCGTCGGGAT AGAAGTGAAG AGCGAGGAGG ACGTCGTCGT GAAGTGGAAT 4381 CGGATGATCG ACGCCGACGT CGTTGAATTT TCAAATTTTA AATACTGAAT ATTTGTTTTT 4441 TTTCCTATTA TTTATTTATT CTCTTTGTGT TTTTTTTCTT GCTTTCTAAA AAATTAATTC 4501 AATCCAAATC TAAacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct 4561 catctgaaca caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg 4621 gggaattggt ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca 4681 atatgcaaca acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata 4741 tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga aattaggcga acgatgagaa 4801 ttgtcctcgc ttggcaaatg cgaatccgta tggagaggca cgtttggcga aggcaaatgt 4861 tcggtatgga gatctgtaaa aatttttaag ttgaaatttg gtgttgctct tttacaaaat 4921 tttccgattt tcgcttgaaa ttacggtgcc aggtctcgac acgtcttcca atttttcaaa 4981 ttcaaaagag cctttaatgg gctgtagttg ctaatttctc gtttttgaaa atttttcttc 5041 cgtttaatcg aaatttgatg tattttattt atgattttca ataaatttca aagaaactgg 5101 tgaaaactcg gaaaattgtg aactacagta atccaatcct taaaggcgca caccttttaa 5161 atgtccgccc caatacgata tttttttaag attcgCTAGA GCGGCCCAAA AGGCCTTTTT 5221 TTTTTGGCGC CGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 5281 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 5341 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 5401 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 5461 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 5521 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 5581 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 5641 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 5701 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 5761 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 5821 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 5881 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 5941 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 6001 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 6061 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 6121 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 6181 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 6241 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 6301 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 6361 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 6421 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 6481 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 6541 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 6601 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 6661 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 6721 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 6781 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 6841 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 6901 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 6961 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 7021 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 7081 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 7141 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 7201 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 7261 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 7321 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 7381 AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt 7441 ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA 7501 ACAACTTGGA AATGAAAT // 1602 LOCUS ADDGENE_1602 3646 bp DNA circular SYN 01/03/2005 DEFINITION L3827 complete plasmid sequence KEYWORDS . SOURCE L3827 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1602 at www.addgene.org FEATURES Location/Qualifiers source 1..3646 /organism="Addgene plasmid L3827" /mol_type="other DNA" CDS 62..1180 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSRQGIQVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGE GEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSR YPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKF EGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLT N*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE KRDHMVLLEFVTAAGITHGMDELYKSPQASGPELDVPRINIIMSRQGIHWPSFYNVVT GKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA" gene 98..946 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 269..319 /label="SynIVS.R" misc_feature 341..360 /label="gfp.S65C" misc_feature 474..524 /label="SynIVS.S" misc_feature 689..739 /label="SynIVS.T" misc_feature 1032..1187 /label="lacZ_a" misc_feature complement(1035..1051) /label="M13f20_primer" promoter 1560..1588 /label="Amp_promoter" gene 1630..2490 /label="Ampicillin" /gene="Ampicillin" CDS 1630..2490 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2645..3264 /label="pBR322_origin" misc_feature 3480..3506 /label="lacI.C" promoter 3573..3602 /label="lac_promoter" misc_feature 3637..3655 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag cttcagggcc cgagctcgac gtcccgcgga ttaatataat 61 tatgagtcga caaggAATTC AAGTACCGGT AGAAAAAATG AGTAAAGGAG AAGAACTTTT 121 CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC 181 TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG 241 CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac taactaaccc 301 tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG TTCAATGCTT 361 cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG 421 TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA CACgtaagtt 481 taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG AAGTCAAGTT 541 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG 601 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC 661 AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac taactaacta 721 atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA 781 ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA 841 CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA 901 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG AcGAACTATA 961 CAAAagccca caagcttcag ggcccgagct cgacgtcccg cggattaata taattatgag 1021 tcgacaagga attcACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 1081 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 1141 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG 1201 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT 1261 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC 1321 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC 1381 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC 1441 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 1501 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 1561 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 1621 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 1681 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 1741 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 1801 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 1861 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 1921 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 1981 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 2041 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 2101 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 2161 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 2221 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 2281 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 2341 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 2401 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 2461 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 2521 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 2581 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 2641 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 2701 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 2761 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 2821 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 2881 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 2941 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 3001 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 3061 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 3121 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 3181 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 3241 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 3301 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 3361 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 3421 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 3481 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 3541 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 3601 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1603 LOCUS ADDGENE_1603 3616 bp DNA circular SYN 01/03/2005 DEFINITION L3828 complete plasmid sequence KEYWORDS . SOURCE L3828 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1603 at www.addgene.org FEATURES Location/Qualifiers source 1..3616 /organism="Addgene plasmid L3828" /mol_type="other DNA" gene 83..931 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 83..1150 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQAFTRPGTAAALKLGIHWPSFYNVVTGKTLALPNLIALQHIPL SPAGVIAKRPAPIALPNSCAA" misc_feature 254..304 /label="SynIVS.R" misc_feature 326..345 /label="gfp.S65C" misc_feature 459..509 /label="SynIVS.S" misc_feature 674..724 /label="SynIVS.T" misc_feature 1002..1157 /label="lacZ_a" misc_feature complement(1005..1021) /label="M13f20_primer" promoter 1530..1558 /label="Amp_promoter" CDS 1600..2460 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1600..2460 /label="Ampicillin" /gene="Ampicillin" rep_origin 2615..3234 /label="pBR322_origin" misc_feature 3450..3476 /label="lacI.C" promoter 3543..3572 /label="lac_promoter" misc_feature 3607..3625 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag ctttcacgcg tcctggtacc gctgcagcgc ttaagttggg 61 AATTCAAGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC 121 AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG 181 TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT 241 ACCTGTTCCA TGGgtaagtt taaacatata tatactaact aaccctgatt atttaaattt 301 tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA 361 TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG 421 AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac 481 taactaacca tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT 541 TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA 601 CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA 661 TGGAATCAAA GTTgtaagtt taaacatgat tttactaact aactaatctg atttaaattt 721 tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA 781 TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC 841 CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA 901 GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGAcGAA CTATACAAAa gcccacaagc 961 tttcacgcgt cctggtaccg ctgcagcgct taagttggga attcACTGGC CGTCGTTTTA 1021 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC 1081 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG 1141 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT 1201 ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 1261 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 1321 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 1381 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 1441 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 1501 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 1561 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 1621 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 1681 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 1741 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 1801 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 1861 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 1921 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 1981 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2041 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 2101 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 2161 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 2221 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 2281 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 2341 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 2401 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 2461 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 2521 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 2581 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 2641 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 2701 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 2761 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 2821 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 2881 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 2941 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3001 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3061 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 3121 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 3181 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 3241 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 3301 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 3361 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 3421 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 3481 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 3541 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 3601 CACACAGGAA ACAGCT // 1604 LOCUS ADDGENE_1604 3700 bp DNA circular SYN 01/03/2005 DEFINITION L3829 complete plasmid sequence KEYWORDS . SOURCE L3829 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1604 at www.addgene.org FEATURES Location/Qualifiers source 1..3700 /organism="Addgene plasmid L3829" /mol_type="other DNA" CDS 26..1234 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSWAHRFGRPEGLSGRVEGIPGYRARIQVPVEKMSKGEELFTGV VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPD YLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRK FKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVY IMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGP VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQAYMSWAHRF GRPEGLSGRVEGIPGYRARIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPA PIALPNSCAA" gene 125..973 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 296..346 /label="SynIVS.R" misc_feature 368..387 /label="gfp.S65C" misc_feature 501..551 /label="SynIVS.S" misc_feature 716..766 /label="SynIVS.T" misc_feature 1086..1241 /label="lacZ_a" misc_feature complement(1089..1105) /label="M13f20_primer" promoter 1614..1642 /label="Amp_promoter" gene 1684..2544 /label="Ampicillin" /gene="Ampicillin" CDS 1684..2544 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2699..3318 /label="pBR322_origin" misc_feature 3534..3560 /label="lacI.C" promoter 3627..3656 /label="lac_promoter" misc_feature 3691..3709 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag cttacatgtc ctgggcccat cgattcggac gtcccgaggg 61 cttaagcgga cgcgtcgagg ggatccccgg gtaccgagct cgAATTCAAG TACCGGTAGA 121 AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA 181 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA 241 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag 301 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC 361 TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA GATCATATGA AACgGCATGA 421 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 481 TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt 541 taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT 601 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA 661 TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag 721 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG 781 ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT 841 TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC 901 GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG 961 GATTACACAT GGCATGGAcG AACTATACAA Aagcccacaa gcttacatgt cctgggccca 1021 tcgattcgga cgtcccgagg gcttaagcgg acgcgtcgag gggatccccg ggtaccgagc 1081 tcgaattcAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA 1141 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC 1201 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT 1261 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC 1321 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC 1381 TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC 1441 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG 1501 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 1561 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 1621 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 1681 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 1741 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 1801 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 1861 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 1921 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 1981 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 2041 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 2101 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 2161 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 2221 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 2281 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 2341 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 2401 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 2461 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 2521 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 2581 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 2641 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 2701 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 2761 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 2821 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 2881 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 2941 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 3001 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 3061 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 3121 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 3181 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 3241 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 3301 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 3361 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 3421 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 3481 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 3541 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 3601 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 3661 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1605 LOCUS ADDGENE_1605 3634 bp DNA circular SYN 01/03/2005 DEFINITION L3863 complete plasmid sequence KEYWORDS . SOURCE L3863 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1605 at www.addgene.org FEATURES Location/Qualifiers source 1..3634 /organism="Addgene plasmid L3863" /mol_type="other DNA" CDS 38..1168 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTSRDDASPWGIQVPVEKMSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYG VQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFS GAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSL NMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL SKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQALHVPLMTSRDDASPWGIHWPSFY NVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA" gene 92..940 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 263..313 /label="SynIVS.R" misc_feature 335..354 /label="gfp.S65C" misc_feature 468..518 /label="SynIVS.S" misc_feature 683..733 /label="SynIVS.T" misc_feature 1020..1175 /label="lacZ_a" misc_feature complement(1023..1039) /label="M13f20_primer" promoter 1548..1576 /label="Amp_promoter" gene 1618..2478 /label="Ampicillin" /gene="Ampicillin" CDS 1618..2478 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2633..3252 /label="pBR322_origin" misc_feature 3468..3494 /label="lacI.C" promoter 3561..3590 /label="lac_promoter" misc_feature 3625..3643 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag ctttacatgt cccattaatg acgtcccggg atgacgcgtc 61 gccatggggA ATTCAAGTAC CGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG 121 AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG 181 TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC 241 TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta accctgatta 301 tttaaatttt cagCCAACAC TTGTCACTAC TTTCTgTTAT GGTGTTCAAT GCTTcTCgAG 361 ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT 421 ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca 481 gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG 541 TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT 601 TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA 661 ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta actaatctga 721 tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC 781 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA 841 TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT 901 CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGAcGAAC TATACAAAag 961 cccacaagct ttacatgtcc cattaatgac gtcccgggat gacgcgtcgc catggggaat 1021 tcACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 1081 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 1141 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC 1201 CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT 1261 GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG 1321 GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG 1381 TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC 1441 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 1501 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 1561 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 1621 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 1681 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 1741 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 1801 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 1861 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 1921 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 1981 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 2041 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 2101 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 2161 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 2221 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 2281 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 2341 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 2401 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 2461 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 2521 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 2581 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 2641 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 2701 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 2761 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 2821 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 2881 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 2941 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 3001 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT 3061 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 3121 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 3181 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 3241 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 3301 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 3361 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 3421 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 3481 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC 3541 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT 3601 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1606 LOCUS ADDGENE_1606 4568 bp DNA circular SYN 01/03/2005 DEFINITION L2528 complete plasmid sequence KEYWORDS . SOURCE L2528 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1606 at www.addgene.org FEATURES Location/Qualifiers source 1..4568 /organism="Addgene plasmid L2528" /mol_type="other DNA" misc_feature 31..53 /label="mec7.-836" misc_feature 843..866 /label="mec7.Tsa" misc_feature 915..956 /label="SynIVS.A" misc_feature 963..980 /label="rf21.28" misc_feature 1043..1093 /label="SynIVS.M" misc_feature 1170..1220 /label="SynIVS.L" promoter 2348..2376 /label="Amp_promoter" gene 2418..3278 /label="Ampicillin" /gene="Ampicillin" CDS 2418..3278 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3433..4052 /label="pBR322_origin" misc_feature 4268..4294 /label="lacI.C" promoter 4361..4390 /label="lac_promoter" misc_feature 4425..4443 /label="M13r_primer" misc_feature 4457..4507 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTctagA AATGTAAACC TGTCATTTCT Gtgtatttca 61 gcaccgcgaa gagcaccata aacagctaca acaagagcac gaacgtcggc tccaacagaa 121 aaagctgcaa aacgctggta ctcaccacat gctctgataa ttgccatttc cttcgatttc 181 tgactttcaa ttgtgatatg ataacctcaa aatctccatt caaaatgaaa actcttaaat 241 ttaagagctt tcaacacccc gcactttccc acctcatcgt ttttcccttc tatcttatca 301 attttttgta ttttgtgtca gtttgctata tcctcatcac cttttttacc gtttaatctc 361 tctatagtat tgttcgggtg tttcaagatg aaacgttttg tgtgtagcat ttttgaaaca 421 cagcgcgaaa aatgcagaaa ctatcatcca caatgcatta agtgcactca ttgtgatgac 481 tcctaacccc ctcgccccac cagttgtctc ttcacaaatt catggcacaa ttcaaaaatg 541 gctttttgtg ttggatcttg atctcgtctc tttccttctt ctttcgtttc tctcggacca 601 ctcattattc gaatgccatg catttgttga tgatgcgcag cgatggtcgc acacactaca 661 acagatatga tggtggctgt gcgtggtgca gagctcatga ataagtgatg gggcaccgag 721 atgtgactcc tcccattatt cgtcttgagt cgcatctttt gtcttggtcc gttgtcagtc 781 gagcctcaag gcagccgttc cgccagtgat cgcctgctaa gaagttgtag gtgtagagga 841 agaaacgtcg ggtccaaatt tcaagCAACA TCCGCGAGAT CGTGAATCCC GGGGATTGGC 901 CAAAGGACCC AAAGgtatgt ttcgaatgat actaacataa catagaacat tttcagGAGG 961 ACCCTTGGCT AGCGTCGACG GTACCGGTCA GATATCACTC GAGGAGCTCC CGAGATCTTC 1021 AGCGCTCAGC ATTCGTAGAA TTgtaagttt aaactttctc atactaacta acgcgctcta 1081 tttaaatttt cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT 1141 CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta ctaactaacg 1201 agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG TCCAATTACT 1261 CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC 1321 AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA 1381 ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC 1441 CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT 1501 ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA 1561 CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG 1621 CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT 1681 TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGTTTCCCCT 1741 ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG 1801 CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA 1861 AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC 1921 ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT 1981 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC 2041 AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 2101 TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 2161 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC 2221 TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT 2281 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 2341 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 2401 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 2461 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 2521 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 2581 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 2641 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 2701 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 2761 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 2821 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 2881 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 2941 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 3001 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 3061 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 3121 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 3181 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 3241 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 3301 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 3361 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 3421 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 3481 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 3541 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 3601 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 3661 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 3721 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 3781 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 3841 ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 3901 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 3961 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 4021 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 4081 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA 4141 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 4201 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 4261 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 4321 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 4381 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC 4441 ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa 4501 ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA 4561 AATGAAAT // 1607 LOCUS ADDGENE_1607 4895 bp DNA circular SYN 01/03/2005 DEFINITION L2531 complete plasmid sequence KEYWORDS . SOURCE L2531 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1607 at www.addgene.org FEATURES Location/Qualifiers source 1..4895 /organism="Addgene plasmid L2531" /mol_type="other DNA" misc_feature 57..81 /label="myo2.-1090" CDS 1032..1679 /label="Orf2" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWLASTVPVRYHSRSS RDLQRSAFVEL*V*TFSY*LTRSI*IFRIPTERRSLPLPTCLVSKIIGAAVIRVSLN* VLLTNE*YLNFQHLAPVPLTSKSNYSSTSLHALSPCAPTPYFCYYQKTSLNFFVF" misc_feature 1145..1168 /label="myo2.5'" misc_feature 1242..1283 /label="SynIVS.A" misc_feature 1290..1307 /label="rf21.28" misc_feature 1370..1420 /label="SynIVS.M" misc_feature 1497..1547 /label="SynIVS.L" promoter 2675..2703 /label="Amp_promoter" CDS 2745..3605 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2745..3605 /label="Ampicillin" /gene="Ampicillin" rep_origin 3760..4379 /label="pBR322_origin" misc_feature 4595..4621 /label="lacI.C" promoter 4688..4717 /label="lac_promoter" misc_feature 4752..4770 /label="M13r_primer" misc_feature 4784..4834 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAG CTTGCATGCC TGCAGGTCGA 61 GGCATTTGAA TTGGGGGTGG TGGACAGTAA CTGTCTGTAA TAATAATTAC TCCTGACCAG 121 GTTGCAATTC GAGTTTTGAT AAGCATAATT ATACCTTGTA CATTGTGGGT TTTGTGCTGT 181 GGACGTTTTA TTGTGACATC CCCATAAGCT ACAAGAACAA AAATGAAATg TAAAAGTATT 241 GAAAACGTCG TACATTTTAT ATCTGAGTAG TATCCTTTGC TTTAAATGTC CATAAAAcTA 301 ATTTTATAAT CAATAAAACA ACGTTTGTAA ATCAACTGAG TTTACAAGTA GAGACATTGA 361 GGGATACTTT CACTATGCTA AAGTGATAAT CGACCAAATA ATAACTtCAC TTTGGTATTT 421 ATTCCTGTCT TATAAaTGTT ATGTATGAAT TAAATTCATA TGCATATGGC TCACTCTGAC 481 AAtttttAAT AATCTTCCAG ATCAATATTG ACTACCGATG CGGGTGGTCT TTTGCTTTGA 541 ATTCTGCTGA ACTTTACACC CCGAACAGCA ATGTGTGCTT CAGCcTAAAA AAAAGTAAGT 601 GTGTTAATCA GTgCCCCCGA TTCTTCATTT TTTGCCCCTC TCTCCCGTTT CGTCGGCAAA 661 AGAAGAGAAA ATAAAGATAA GTCTCAAGAT AGGTTGGTAA TCGCTAAAGT GGTTGTGTGG 721 ATAAGAGTAG CAAAATGGCA GGAAGAGCAC TTTGCGCGCA CACACTGTAC TCATTGTTCT 781 GGATAAAATT CTCTCGTTGT TTGCCGTCGG ATGTCTGCCT CTCTGCATTG AGCCGGCTTC 841 TTCACTATCT TTAGTTAACC TAAAATGCCG TTTCTTTTCT CGTATCCCcA CTATcCCGTT 901 GAGGTTCTCT GCTCTCTTCG CTCCCTACCG CCAGCGAGCA ACTATCCGTG GGGGCGCCTT 961 GCTCGGAAGA TGGGGGGGAA GAAAGAAGAT TTTTGCTATT TGCACTTGAG AAAGAGACTT 1021 TTCCTGCGTC GATGGTTAGA GAACAGTGTG CAGACACTTT TCAGCTACCT AGAatTACAa 1081 tTGGATATCC CCGCCTCCCA ATCCACCCAC CCAGGGAAAA AGAAGGGCTC GCCGAAAATC 1141 AAAGTTATCT CCAGGCTCGC GCATCCCACC GAGCGGTTGA CTTCTCTCCA CCACTTTTCA 1201 TTTTAACCCT CGGGGTACGG GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT 1261 AACATAACAT AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCGGTCAGAT 1321 ATCACTCGAG GAGCTCCCGA GATCTTCAGC GCTCAGCATT CGTAGAATTg taagtttaaa 1381 ctttctcata ctaactaacg cgctctattt aaattttcag AATTCCAACT GAGCGCCGGT 1441 CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGAgtaa 1501 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagCAT CTCGCGCCCG 1561 TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG 1621 CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC 1681 TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA 1741 ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT 1801 CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC 1861 ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG 1921 CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC TCATCGTGAA 1981 AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA ATTAATTTTC 2041 CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT 2101 CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG AAGGTTTGGG 2161 TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT AGTGTCTATG 2221 GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC TGTTTCCTAC 2281 TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG 2341 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA 2401 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC 2461 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT 2521 AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG 2581 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 2641 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 2701 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 2761 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2821 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2881 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 2941 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 3001 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 3061 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 3121 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 3181 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 3241 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 3301 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 3361 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 3421 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 3481 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 3541 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 3601 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 3661 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3721 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3781 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3841 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3901 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3961 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 4021 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 4081 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 4141 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 4201 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 4261 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 4321 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 4381 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 4441 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 4501 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 4561 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 4621 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC 4681 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 4741 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat 4801 cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA 4861 CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1608 LOCUS ADDGENE_1608 6087 bp DNA circular SYN 01/03/2005 DEFINITION L2534 complete plasmid sequence KEYWORDS . SOURCE L2534 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1608 at www.addgene.org FEATURES Location/Qualifiers source 1..6087 /organism="Addgene plasmid L2534" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" misc_feature 2482..2499 /label="rf21.28" misc_feature 2562..2612 /label="SynIVS.M" misc_feature 2689..2739 /label="SynIVS.L" promoter 3867..3895 /label="Amp_promoter" CDS 3937..4797 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3937..4797 /label="Ampicillin" /gene="Ampicillin" rep_origin 4952..5571 /label="pBR322_origin" misc_feature 5787..5813 /label="lacI.C" promoter 5880..5909 /label="lac_promoter" misc_feature 5944..5962 /label="M13r_primer" misc_feature 5976..6026 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGGTATGTT TCGAATGATA CTAACATAAC 2461 ATAGAACATT TTCAGGAGGA CCCTTGGCTA GCGTCGACGG TACCGGTCAG ATATCACTCG 2521 AGGAGCTCCC GAGATCTTCA GCGCTCAGCA TTCGTAGAAT Tgtaagttta aactttctca 2581 tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA 2641 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 2701 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 2761 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 2821 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 2881 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 2941 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 3001 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 3061 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 3121 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 3181 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 3241 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 3301 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 3361 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 3421 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 3481 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 3541 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 3601 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 3661 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 3721 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 3781 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 3841 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 3901 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 3961 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 4021 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 4081 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 4141 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 4201 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 4261 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 4321 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 4381 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 4441 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 4501 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 4561 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 4621 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 4681 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 4741 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 4801 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 4861 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 4921 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 4981 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 5041 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 5101 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 5161 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 5221 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 5281 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 5341 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 5401 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 5461 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 5521 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 5581 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 5641 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 5701 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 5761 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 5821 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 5881 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 5941 ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa 6001 ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG 6061 GATACGCTAA CAACTTGGAA ATGAAAT // 1609 LOCUS ADDGENE_1609 11864 bp DNA circular SYN 01/03/2005 DEFINITION L3920 complete plasmid sequence KEYWORDS . SOURCE L3920 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1609 at www.addgene.org FEATURES Location/Qualifiers source 1..11864 /organism="Addgene plasmid L3920" /mol_type="other DNA" misc_feature 1839..1864 /label="mex3.Tsa" gene 2500..3360 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2500..3729 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNLDLLTNGLYLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVRSRSPRRSSARSPSRSPRRRRERSSERKQSEEPAPLPEKKKK EPLDILRTRTGGAYIPPAKLRLMQQQISDKQSEQYQRMNWERMKKKIHGLVNRVNAKN LVQIVRELLQENVIRSK" misc_feature 2671..2721 /label="SynIVS.R" misc_feature 2743..2762 /label="gfp.S65C" misc_feature 2876..2926 /label="SynIVS.S" misc_feature 3091..3141 /label="SynIVS.T.notcorrect" CDS 7521..8225 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 8201..8225 /label="let858.C" misc_feature 8303..8332 /label="let858.3'" promoter complement(9049..9067) /label="T7_promoter" misc_feature 9072..9232 /label="lacZ_a" misc_feature complement(9075..9091) /label="M13f20_primer" rep_origin 9250..9556 /label="f1_origin" promoter 9750..9778 /label="Amp_promoter" gene 9820..10680 /label="Ampicillin" /gene="Ampicillin" CDS 9820..10680 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 10835..11454 /label="pBR322_origin" misc_feature 11670..11696 /label="lacI.C" misc_feature 11827..11845 /label="M13r_primer" promoter 11862..11881 /label="T3_promoter" ORIGIN 1 TAACCCTCAC TAAAGGGAAC AAAAGCTGGG GGGGATCCCG CGTATTGCAC AACATATTTG 61 ACGTGCAAAA TATCTAGTAG CGAAAACTAT AGTAACTCTT TAAATGACTT TTGTAGCACT 121 GATGCCGATT TAATTAAATT ATTTATTAAC AGATAAAATA TTAAAAGAAA ACACGACAAT 181 TCAAAAAAAT TCATTTCGTA AATCGAGCCC GTAAATCGAC ACAAGCGCTA CAGTAGTCAT 241 TTAAAGAATT ACTGTTTTTT CGCTACGAGA TATTTTGTGC GTCAAATATG TTGCGCAATA 301 CGCATTCTTA TAATTTTGTG TACCCGTAGT ATATTTTTGT CTCTCTGATT TCCATTGCAA 361 TTTTTTAATT CCATATTTTT TTGGTGACAA ATTTCTCCGC TTATCAATTT TTTTTTCAAC 421 CACATAATTA TTAACCCCAA AACATCGCAG ATTTTCAGGT AAAATGCCAG TCGTGAGCGT 481 TAGACCTTTT TCTATGAGAA ATGGTGAGCA ATTTTTTTGA AGTTTTTTTT TGAAATTTTT 541 AATTTTTCCA GAAGGCTTCT CAAACGGGCA TAACACGTGG AACGACGCCG AATTTTGGAA 601 ACCGTGAGTT TTCGGCAAAT AAGCTAACAG TCTAAGAAAC ACGAAAAAAA ATCCAATTTT 661 CTTTTCAAAA TTCGTGATTT TCGGATGATT TTTGAACTTT TTGATTAATG TTCCCACTGA 721 AAATCCTCCA AATCTCTAAA AATCCGCTTC TAAAAAAGCC TCAAATGGCC TTGCGAGAAT 781 AGACGGAATG TTCAAGGATG ACTGGTATAA AATCGGCAAA CGTGTTGCCA TTCAAACAGA 841 ATATCTCGAA ATTTCGCCTT CCTTTTTTTT AAGTGGGTCG GATATTTAAC ATACAGAAGA 901 GAATAGGGAG AGAAAAGAGG GAAAAGAGGG GAAAAGTCTT CTTGGAAACA ACCTCGAACC 961 GCAATTAAGT TTTTGCCTCA CCCTTCAATT TTCGTGAGAA AGTTAAAAAT CCAAATTTTT 1021 TTCTCCTTTT TTAAAAAAAG ATTTCCTCCT CAAAAATTCA AAGGATCGAA TTTTTTGTTA 1081 CACGCCTGCC TCCTTTTTAT TACCCTTGTT CTTGATCGCA ACCTCTTTTC TAGATTACTT 1141 GAAAAAATGC GCTATTTTTT TTCCTTTTTC AACAGAGTCA ATTTCAAAGG TGCTGTGGAA 1201 AAAGTTTCGG TTTTTTTTTG CAAAAATAAT CGAAATTTGT TCGTTTATAC TGCTTAAAAA 1261 ATGACCAAAA TTTCAAGTAA TTCATTCGAA AAATCGATTT TTTTGAAATA TACAAAAATT 1321 TTCAATTTTT CATATCTGAA AACACAATTT TTTCGATTTT TCATTTTTCT TGGAAAAAAC 1381 ACAAAAAAAA ACTGTATTTA ATTTCCCACT TAATACTACG ATATCCAAGC CCCGCCTCCG 1441 CGTATTGTAC GTGAACACGG CGGCCCAAAA TTACACACTA TCTCACACAC GTTAAATGAG 1501 TGATACCGTA CTCTCTCTGT TTTTTCTTTC ATTTTTTCGG CGCCGAAAAT TTTCAATTTT 1561 TGGTGGTTCT TTTGGCGGGA TTTTTCGGAT TTTTGTCGCG TTCACACACA CACACCAGTT 1621 GGTGTTGCCT GTCGTCACCC ACCCGCCCCA CTCTTTGTCC GTGTACTGCT TTGCCATTTG 1681 TGCGCGCGTA CCGCTGCCCG TCTGTCTTTC TCATCATATG CGCCTCGTTT TGTCGCTTTT 1741 TCTCCATTTG CCCTTCCTAC ATGGGTACCT GCGGTTTTCA TCGAAAATAT CCTTAAAAAT 1801 TCCGAAAAAC CTCTATTTTT CGATTTTTGA ATCGATTTTC GCCCAAACAT AACCATTTTT 1861 CAGAataaaC CTAGGGGATA AGATGTCGTC CCgggttcta gaagcgctgc taagggggcc 1921 cTCGTCGAGT CGGTCACAAT CACCTGAAAC TCCAAAGGCA GCCAGTGAGG AACGTGAAGA 1981 AGAAGAAAAA GAGTCATCTG AACAGgtttg attttctttc tggtcaaaaa gatgaaatta 2041 ttgattttca gCCAGATACT CCCAAAACTA GCAGCGAGAA GTCTGCAAGT CGTTCACAGT 2101 CGCCCAGAGA ATCGCGGGAA GTGAGCCAAG AGgtatgttt ttcaaaaatc aataactgat 2161 cataattttt attgtttggt gaatttaaga aaataatatt cgaaaattcc tctgaattat 2221 caagattgca gtattaattt cgagaaaaat tgagatattc atagagctat tgtaaatttt 2281 cttgatttca gACTGAAACT TCGGAAAATC AAGAGAAAAT CAAAGAAAAG GATGACGGGG 2341 ATGATCAGCC TGGCACACCG AACAGCTATA GAAGCCGGGA AACTTCACCA GCTCCAAAAA 2401 GGTCCAAGGA GACCAGgttt gtcaaaagct tcctgcgatt aattctcatt tcaatttttc 2461 agAGAATCAG AGTCTCCTGA AAAATCACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT 2521 TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT 2581 TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT 2641 TGCACTACTG GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac 2701 cctgattatt taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC 2761 TTcTCgAGAT ACCCAGATCA TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA 2821 GGTTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag 2881 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG 2941 TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT 3001 GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG 3061 GCAGACAAAC AAAAGAATGG AATCAAAGTT gtaagtttaa acttggactt actaactaac 3121 ggattatatt taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT 3181 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA 3241 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC 3301 CACATGGTCC TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA 3361 TACAAATgcC CGGTTCGTTC AAGATCTCCC AGAAGGTCTT CAGCACGTTC CCCGTCACGA 3421 TCTCCTAGAC GGCGCCGAGA AAGAAGCTCA GAAAGAAAGC AATCCGAAGA GCCAGCACCG 3481 CTACCAGAGA AAAAGAAGAA AGAGCCGCTG GATATTCTAC GAACAAGAAC CGGAGGAGCA 3541 TATATTCCAC CCGCCAAACT TCGACTTATG CAACAACAGA TTAGTGATAA GCAAAGTGAA 3601 CAGTATCAGA GAATGAATTG GGAAAGAATG AAGAAAAAGA TTCACGGATT GGTTAACAGA 3661 GTCAACGCGA AGAATCTTGT TCAAATTGTC AGAGAACTTC TTCAAGAGAA TGTGATTCGT 3721 TCAAAgtgag tgagaaaatc gaaggaaaag gaaagaatta atttaatttt tcagGGGACT 3781 TCTCTGCCGT GACATTATTC AAGCTCAGGC TTTCTCACCA GGATTCTCTA ACGTCTATGC 3841 AGCTTTGGCG GCAGTTATCA ACTCGAAATT CCCTCATGTC GGTGAACTTC TTCTCCGTCG 3901 TCTGATTGTA CAGTTCAAAA GAAGTTTCCG TAGAAATGAC AGAGGCGTCA CGGTGAACGT 3961 GATCAAATTC ATCGCACATT TGATTAATCA ACAAGTTGCT CACGAAGTTC TTGCGCTGGA 4021 AATCATGATT CTGATGCTTG AAGAACCAAC TGATGATTCA GTTGAAGTCG CCATTGCGTT 4081 CCTGAAAGAG TGTGGAGCAA AGCTTCTGGA GATTGCTCCA GCAGCTCTTA ACAGTGTCTA 4141 CGACCGTCTT CGTGCAATTC TCATGGAAAC TGAAAGATCG GAAAATGCAC TGGATCGACG 4201 TATTCAGTAT ATGATTGAGA CTGCAATGCA GATTCGAAAG GACAAATTTG CGgtaaggta 4261 gaatatataa atagtttatt agaaaaaaat aaattagaat aatttaaatt cctactagcc 4321 aatcaggcga cctttttgcg catagttcta ttattgaaaa atttggagaa tttctcatat 4381 tctcgctcgg aaatctggaa ttcgacgaga tcttctggct tctgtgcagc tgcatcgctt 4441 tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa ccttgttgag ttcatcaact 4501 gaatctgtga ctggcttgtt gctcactgga tgcactagac gactgattct cgagaaatca 4561 gattgagttg cgattagggt gacctagaaa ttgggaataa tacgaacttt tgaaaatatt 4621 caggaggatt aaaaaaatta ttctcgacaa tcctacaaat ttacttattg caccatgttg 4681 ctccaacatt tttcattaaa agttaatgaa aaaatgtaga aaatcggaaa ttggcaattt 4741 tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct gaaataaatg tcattttcag 4801 ataaatcgag cgattttctg ttgtctgaca ctagttttta gttttaaaaa atgttggaag 4861 aacatggtgc aataggtaat ttcatagaat ttccatgtgt tttttttcaa ttaaccaatt 4921 atccaaatct tccaaactca cattttgcgg agctgggcta tcaagaatct gctgcagttt 4981 tataagacga gcatctctga tatcactgaa aattaatttt taatcaaaac ttgaatatca 5041 actaaaccca cttattaact ttctcgatct tctgtcgttc ggtacgatga cggtgaagaa 5101 gccaattgta gtagttgatt tggttcaagt cctttcggtg ttgtacgtca gtgtcctgca 5161 atgctattta gttataactt aggcctaaga ttcaatttaa tgaagtgatt aaatttgttc 5221 tctgaacctc ttaagatgat cttttggatt agaaacatat aagacaggtt tacctatcta 5281 ttaaaaaaca gatcaaaata gatacgacca aatcggataa tccatgccta cctggcatct 5341 aggaacgtgt tcttagaaga tttcttacgt aatcgtatga agaaataaca atttgatcgt 5401 tggccagcaa aaatagggtt ttaagtggga tagtgttttt attagctaac cggaaaattt 5461 tatagttttt ttttgcaaga aaccactgaa aaccccctaa ttgtatacat tttttggagc 5521 agcttctggt ctttttgagc aataaaattc gataaaacag aatttaagtg taaattgttc 5581 acatttagtt tctattttat caaattttgt tgctcaaaaa cattcgaagc tgctctaaaa 5641 aaatgcatta aaaaaggggt tttcagtggt ttttcacatt aaaaaagcta attttaacta 5701 aaaatccatc atatttccaa ctttgtcaca acaataaaat gctggtcaaa atgtgttcga 5761 aaaaatgttt ttttttttaa tttttataat ttaaaaatag ttttctttcg ctgggacaca 5821 tacatttttg ggcgtaaatt ttcagttcaa atttccattt ttacaaccat aatcataaag 5881 ctacgtctga tctctctcgc acttacctgc gcctgattcg aaagaacaac cgtagccaaa 5941 agaacaagaa gaacaagcac gtagttgtgg tagtggacgt tcatcacgca atactgacca 6001 atggtcgtgg ggtctcactt tccgtactat tgagagaggg gagactgaag atggcaattg 6061 aggacagtgt cttcgacgca cgcatgcatc cataagcata atccaggagg gatggagaga 6121 aaaatcttgt ttctaagccc ctccctttgt aatacataca catatctaat accgaagaat 6181 ggctaattga atggacgtca gctgttgctg tagttgccaa ggcatcatcg atgaaataac 6241 tgaaagaaag aattaaataa ttattgcagG CGTATCCGGC GGTCATTGAA GACTTGGACT 6301 TGATTGAGGA GGAGGATCAG ATCATCCATA CACTTAATTT GGAGGATGCG GTTGATCCGG 6361 AAAATGGGCT TAgtaagtga ctgaccacac gcggggggca ttaatttaat aaattgaatt 6421 ccatttcagA TGTGTTCAAA CTAGATCCAG AATTCGAAAA GAACGAGGAG GTTTATGAGG 6481 AGATCCGTAA GGAAATCATT GGAAACGCCG ATATTTCGGA TGAGGATGGT GGCGACGAGT 6541 TGGATGATGA AGAAGAGGGT AGTGATGTGG AAGAGGCTCC GAAGAAGACT ACAGAGATTA 6601 TTGATAATAC TGATCAGAAT TGACTGCTTT CAGAAGgtat tcattttgag ttttgggccg 6661 gcaaatctgt aagttgccgg ttgccgaaaa tttgctgaat ttgccggaaa aaaaaattcc 6721 ggaatttatt taaaaacttt ttgtaaaaat taaattaaat ttgcaacttt tcagAGAAGT 6781 CTACCTGACA ATGCAATCAT CTTTGGACTA CCAAGAAGCT GCTCACAAAT TGCTGAAAAT 6841 GAAGATTCCA GACAGCATGC AGgtcagcga tgttgcaaag aaaaattttc gaccaaaaaa 6901 accaaccaat cataaaattt aaaaaaaaac tccgtttttt tctttttttt tatacgagaa 6961 aaaccaaaaa aatgtatttt tgccaaattc taaaatacta tccccgaaat tttcaatatt 7021 ttctctttca gAACGAACTC TGCGCGATGC TTGTCGATTG TTGTGCTCAA CAGCGTACCT 7081 ACGAGCGATT CTACGGAATG CTCATCGAAC GTTTCTGCCG ACTTCGCCTC GAATACCAGC 7141 AATACTTTGA AAAGCTCTGC CAGGACACGT ATTCCACGAT TCACCGAATT GACATCACAA 7201 AACTGCGGAA TTTGGCTCGC CTTATTGCTC ATTTGCTCTC GACGGATGCT ATTGACTGGA 7261 AGATTTTGGC CGATATGAAA ATGACCGAAG AGGACACAAC TTCTTCTGGC AGAATCTATA 7321 TTAAATATAT ATTTAATGAA CTTGTGGAGG CGATGGGAAT GGTTAAACTT CATTCGAGAG 7381 TTACTGATCC gtgagtttcc tagagagagt tgttttcgta ttcaattttc cctattttca 7441 gAACTTTGGC TCATTGCTTT GTTGGATTAT TCCCACGAAC TAATCCGAAC AGCGCACGAT 7501 TTTCGATCAA CTTCTTCACA ATGATTGGAT TGGGTGGTTT GACGTTGGAA CTTCGTGAAT 7561 GGCTGGCAAA GGGTCTCAAG AAGAAGAAGG GAATGCTGGA TCAGTTGAAG GCCGAATCAA 7621 GCTCAGATTC ATCGTCGTCT TCGGATTCGT CAGACTCGTC TGATTCTTCG GATTCTGACG 7681 ATTCATCCGA CTCGTCTTCA GATTCCTCAT CTTCTTCAGA ATCAGAGCCA GAACCACCGA 7741 AGAAAAAGAA GAAGAAGAAC AGTGAAGAGA GTTCCAAAAA GAAGGAAAAA GAGAATATTG 7801 GTCGACGGGA TCGTGGAGAC AAGAGAGCTG AACGTCATCG TGATCAAAGT GTGGAGAACA 7861 AGGACAAGGA TCGTCGACGT CGCCAGGATT CTGACGAAAA TCGTCGGCCA GAACGAGGAG 7921 ATGACCGCAA GGATCGGAGT AAAGATCGTC GTCGTCAAGA CTCGGATGAT GAGGATCGGA 7981 AAGGTCGTGA ACGTCGGGAA GATTCAGGGG AAAGACGTCG CGGAGATCGG GATCGACGTG 8041 ATCGAAACAA GGATCAGGAG GATCACCGTG AAGATCGCCG TGACCGAAGC AAGGATCGTG 8101 AGGATCGACG TGATCGCCGT CGTCATGACT CTGATGATGA TCGTAAAACT CGTCGGGATA 8161 GAAGTGAAGA GCGAGGAGGA CGTCGTCGTG AAGTGGAATC GGATGATCGA CGCCGACGTC 8221 GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC 8281 TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt 8341 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag 8401 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa 8461 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc 8521 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact 8581 agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tggcaaatgc 8641 gaatccgtat ggagaggcac gtttggcgaa ggcaaatgtt cggtatggag atctgtaaaa 8701 atttttaagt tgaaatttgg tgttgctctt ttacaaaatt ttccgatttt cgcttgaaat 8761 tacggtgcca ggtctcgaca cgtcttccaa tttttcaaat tcaaaagagc ctttaatggg 8821 ctgtagttgc taatttctcg tttttgaaaa tttttcttcc gtttaatcga aatttgatgt 8881 attttattta tgattttcaa taaatttcaa agaaactggt gaaaactcgg aaaattgtga 8941 actacagtaa tccaatcctt aaaggcgcac accttttaaa tgtccgcccc aatacgatat 9001 ttttttaaga ttCTAGAGCG GCCGCCACCG CGGTGGAGCT CCAATTCGCC CTATAGTGAG 9061 TCGTATTACA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 9121 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCCTTCGCCA GCTGGCGTAA TAGCGAAGAG 9181 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGTAGCCTGA ATGGCGAATG GGACGCGCCC 9241 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 9301 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 9361 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 9421 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 9481 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 9541 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 9601 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 9661 TTTAACAAAA TATTAACGTT TACAATTTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 9721 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 9781 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 9841 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 9901 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 9961 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 10021 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 10081 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 10141 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAAGCATG 10201 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 10261 GCTTTTTTTC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 10321 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 10381 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 10441 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 10501 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 10561 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGCAG TCAGGCAACT 10621 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 10681 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 10741 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 10801 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 10861 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 10921 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 10981 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 11041 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 11101 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 11161 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 11221 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 11281 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 11341 GGGAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 11401 TTTTTGTGAT GCTCGTCAGG GGGGCCGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 11461 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 11521 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 11581 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 11641 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 11701 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTACC TCACTCATTA GGCACCCCAG 11761 GCTTTACACT TTATGCTTCC GGCTCCTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 11821 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCG GAAT // 1610 LOCUS ADDGENE_1610 8778 bp DNA circular SYN 01/03/2005 DEFINITION L3927 complete plasmid sequence KEYWORDS . SOURCE L3927 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1610 at www.addgene.org FEATURES Location/Qualifiers source 1..8778 /organism="Addgene plasmid L3927" /mol_type="other DNA" misc_feature 17..42 /label="mex3.-5064" misc_feature 5057..5082 /label="mex3.Tsa" misc_feature 5125..5166 /label="SynIVS.A" misc_feature 5173..5190 /label="rf21.28" misc_feature 5253..5303 /label="SynIVS.M" misc_feature 5380..5430 /label="SynIVS.L" promoter 6558..6586 /label="Amp_promoter" gene 6628..7488 /label="Ampicillin" /gene="Ampicillin" CDS 6628..7488 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7643..8262 /label="pBR322_origin" misc_feature 8478..8504 /label="lacI.C" promoter 8571..8600 /label="lac_promoter" misc_feature 8635..8653 /label="M13r_primer" misc_feature 8667..8717 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGATGG AATTGGTGAC CTAGAAAATA TTTTTTTGAA ATTTCTGCTC 61 CACCAATTTC AATTATAGCA TAACTCATTT CATAGTTGTA GGACGGTGAT TGAAATTTAG 121 GATAGACAGA TTTTAAACCA AAACAGATTG ATGGCCCTTG AAATTTCTAG GCCACCTCTC 181 CAGAAAAAAA TCTGAAAATT GTTTTTATTT CTAATTCATT TTCTTCGTCC GTTTTTCATC 241 TCTCGAGGAT CTTATCCCCT TGTCTTACGT CTCCCACCCG CTGCTATTTG GGCGACCTTC 301 ATAACTGTGA AAATTCTCAC CATTCTCCCT CTCTCGTTTT TGCCCCCCCC CCCCGGTCTC 361 CTATCATTGT TCAAGTACAC CTGTTGTCCC ATAACATCTG CCAACCAATT TTTTCCAATT 421 TTGCCACAAT TTTTATTCAA AAAAGTGCAT TGCAGCGGCA GGCATGCCTC ACTCGTACAC 481 ACACTTGAGC ATACAGGAAA AGCGAAAAAA TCAGTGATTG TTTGGATTCC TGGCTCTTTC 541 TAGGAAAAAA ACTCTTCCAT TTTTGTAGTG CACATCAAAA TGATCAAGCT GGTTAATTTT 601 CAAGTGACTC CCAAAAAAAT AACTGAGAAA GTTGCGTTTT AAAGTTTCTA GGCCATTGTG 661 GGATTTCTAG GTAGGTTTTT GAAAATAGAA GACAGTCTTG CACTAAAGTT TTGATTAGGC 721 TGTTAAATGA TTTTTGTCAG TACGTTTTTT GGAACTTCGA TTTTTGCCAA AAATTCCTTT 781 TTTAGTTTTT GTTTCAGTTT TTTGCAAATT TTTATTTCTT CCATATCTTA CAACATTTAG 841 AAGGTCTCAA AGTCTCTGAT AGCCAAGAAT TGACTCTAAT GACGTTATGA GCTCCGAGAC 901 TATTTTAACC TTTTTCCCTT TTAAGCATAA AATATATAAA TATCAAAAAT TTAATACAAA 961 AACTTCTCGT TTCGAAACGT TTCGAAATCC TCCAGTTAAA AAAAAATTAG ATTACGGCAT 1021 CGGAAAAATT GCGAAACTTT TGAATCTGAG GGATTTTCAA TAGAGGGTCC CGCCAGGAAT 1081 TTGAGTTTTT AACAGTACCC TTTCCATCGT CCTTCCCGAA GCCCATTACA TCATCATTAA 1141 TTTTGCGACC TTTCTAAAAG CGGTCATGGG GGGGGGGACA TGTGTTTGTT GCATATACAG 1201 GGACCCCCCG CCACAACCAC CGCAACTAGA GATAAAGATC TGCTGCACAC AGACAGCTGG 1261 CACATGGATG GACAGAATGT CTCCTTAATT TTACCGCCTA TTTCATAATA ATAAAAGTAG 1321 AACGGGGGGC AGAAAAGAGA ATGATTGCGA AACCACCTGC TTGGCTGTCA GCGCATAAAC 1381 TTTTCGGAAA TTTTGGGTCC CACCACGCTT TTTTCGGTAA CAAATTTTGA GATTTCTCGA 1441 TACACCATGA TGTCTTTTGG TGGAATTTTG GGTTATCATG GCTAATACAA AGCTTTAACG 1501 TGCCTTGAAC TTTCGCAATT TGAACTTTTG GCCTTTACTT AAAAATTTCG ATGGGGCTCT 1561 ATAAGCCATG TGGTTTCGAA CTTTTCCTGA AAATTTAGAT TTTTGTTCAT GCACCATGTT 1621 GTTTTTTTTT TGGAATAATT GAGGAATTCC TTACTTTTAA AGTAAAAAGT TTGGTGAGAT 1681 CCATGTGTTT TCATATTATT CAGTTGGTAA ATTATACAGA TTATTGACTG AAGGTTAATT 1741 TTTTGTTGGG AAAATAAAAA TCGCCAAAAT CAATAAAATC GTATTTAGAA GTAGGGTTTC 1801 CATATAGGCG GTAAAAGGCT TGTCTGCCTG ACAATAAGAC GACCGCCGCT CGCCTCAATC 1861 CGCGGCGGAG GCAGGCAGGG TTCAGGTAGG CACTGAAACC GCGCCTGCTT ACCATGATGT 1921 TCCGTTCTTG TCGATGCACC ATGATAATTT TGGGTTACAT TTTGAGCCTT TTCGAAAAAT 1981 TAATGGTGGA AAATTTCAAG CTGAATTTTA ATATTTATTT TTTTAACCGT ATTGTGGGAC 2041 ATGCAAATGT CTTTCATTTT GTGTCCCTTC ACTCTAACGT CTTCAGAATT TTCGAACTTC 2101 TCGATGCACC ATAATGCTGT TTGGCGATAA CTTTAGAGTA AGAATGGTAG CGATTTTTGC 2161 GATGCACCAT GACGTTTTTA TCGATTTTCA GAAAAAAAAT CACTCCGAGA CCCAAATAGT 2221 TTCATTCTCG ACGCACTATA ACGTTTTCGA AGTAAATTTC AAAATTGCTT CTCGGAAAAG 2281 TTTTTTGAGA CCTACATGCA CCTTTAATCT TCTTTGGAAA CTTTGGAATT TCCGCCGAAT 2341 ACTCTCATTT CAGTTGTTCT CGATGCACCA TGATGGTATT TTTTTCAAAT TACTTTTTTT 2401 TGATTTTTCC ATGAGAGGAC CAATATCTGT CTTTATAACG GCGCATTCTG TAACCAAATT 2461 TTGAAATTCC ACGACATTCT GCTCTCATCG ATTCAACATG ATAATTTTAA AATGAAATTT 2521 AGCGATTTTT CGGGAAAAAA ACAGTTTCGA GATTTTCTAG TTATTGGACA CTCTGCACCT 2581 TGCTTATTTG GAAATTACAT TTTAAACCGA CAACCCTTCA AAAACTGCTT TTCGTCGATG 2641 CACTATGAGT GCATAATTTT GTGTAAATCG TGGTGGGCCT CTAAAAAAAC CGAAAAAAAA 2701 AACGTATAAA ACGCAAACAA ATTGTTTTTG TTGTAATGTT GACACCAACA GATTCGGCGT 2761 GGAATATACG GAAGATCGGA TTTCCAAAAA AACAACCGCT CTCCTGTTTT CGACGTTCCG 2821 CCGCTTTTTA TGGGAGAAGT AGTATGTACC TTTAAGGGTA CTGTAGCCCC TTGAATATTG 2881 GCCTAAAAGT GAAACTAGTG TTGCCAAAAA AAAACGAGAT GACCAGTTTC AATATGTCAT 2941 TTACATCTAC ACATTTCTAA AATCTCATTT TAAATTCCAT TGTTTTCTCG TTTCTTCTGT 3001 CCCAAAGTAC CGTATCCTCA TCCTTCTACC ACCTTAATTT GCATTTCTTC TTCCTTATTC 3061 ACTCTTCTTC CCCTGGACGA AAAATGTTGC ACGACAACAA CAAAAGAGAC CCGCCCCCCA 3121 CCATCGGTTT CTCTGAACCT CTAACTGTCC CCGCCCCCTT TTGGGAGCCC ACTGTCTTTT 3181 CTTTTTTTCT TTGGTCGCTG ATTATGACGG ATCGTTTTTG TCGTTTTTGT ATTACAGGAA 3241 TACAAATTTC CCAAAACGCG TATTGCACAA CATATTTGAC GTGCAAAATA TCTAGTAGCG 3301 AAAACTATAG TAACTCTTTA AATGACTTTT GTAGCACTGA TGCCGATTTA ATTAAATTAT 3361 TTATTAACAG ATAAAATATT AAAAGAAAAC ACGACAATTC AAAAAAATTC ATTTCGTAAA 3421 TCGAGCCCGT AAATCGACAC AAGCGCTACA GTAGTCATTT AAAGAATTAC TGTTTTTTCG 3481 CTACGAGATA TTTTGTGCGT CAAATATGTT GCGCAATACG CATTCTTATA ATTTTGTGTA 3541 CCCGTAGTAT ATTTTTGTCT CTCTGATTTC CATTGCAATT TTTTAATTCC ATATTTTTTT 3601 GGTGACAAAT TTCTCCGCTT ATCAATTTTT TTTTCAACCA CATAATTATT AACCCCAAAA 3661 CATCGCAGAT TTTCAGGTAA AATGCCAGTC GTGAGCGTTA GACCTTTTTC TATGAGAAAT 3721 GGTGAGCAAT TTTTTTGAAG TTTTTTTTTG AAATTTTTAA TTTTTCCAGA AGGCTTCTCA 3781 AACGGGCATA ACACGTGGAA CGACGCCGAA TTTTGGAAAC CGTGAGTTTT CGGCAAATAA 3841 GCTAACAGTC TAAGAAACAC GAAAAAAAAT CCAATTTTCT TTTCAAAATT CGTGATTTTC 3901 GGATGATTTT TGAACTTTTT GATTAATGTT CCCACTGAAA ATCCTCCAAA TCTCTAAAAA 3961 TCCGCTTCTA AAAAAGCCTC AAATGGCCTT GCGAGAATAG ACGGAATGTT CAAGGATGAC 4021 TGGTATAAAA TCGGCAAACG TGTTGCCATT CAAACAGAAT ATCTCGAAAT TTCGCCTTCC 4081 TTTTTTTTAA GTGGGTCGGA TATTTAACAT ACAGAAGAGA ATAGGGAGAG AAAAGAGGGA 4141 AAAGAGGGGA AAAGTCTTCT TGGAAACAAC CTCGAACCGC AATTAAGTTT TTGCCTCACC 4201 CTTCAATTTT CGTGAGAAAG TTAAAAATCC AAATTTTTTT CTCCTTTTTT AAAAAAAGAT 4261 TTCCTCCTCA AAAATTCAAA GGATCGAATT TTTTGTTACA CGCCTGCCTC CTTTTTATTA 4321 CCCTTGTTCT TGATCGCAAC CTCTTTTCTA GATTACTTGA AAAAATGCGC TATTTTTTTT 4381 CCTTTTTCAA CAGAGTCAAT TTCAAAGGTG CTGTGGAAAA AGTTTCGGTT TTTTTTTGCA 4441 AAAATAATCG AAATTTGTTC GTTTATACTG CTTAAAAAAT GACCAAAATT TCAAGTAATT 4501 CATTCGAAAA ATCGATTTTT TTGAAATATA CAAAAATTTT CAATTTTTCA TATCTGAAAA 4561 CACAATTTTT TCGATTTTTC ATTTTTCTTG GAAAAAACAC AAAAAAAAAC TGTATTTAAT 4621 TTCCCACTTA ATACTACGAT ATCCAAGCCC CGCCTCCGCG TATTGTACGT GAACACGGCG 4681 GCCCAAAATT ACACACTATC TCACACACGT TAAATGAGTG ATACCGTACT CTCTCTGTTT 4741 TTTCTTTCAT TTTTTCGGCG CCGAAAATTT TCAATTTTTG GTGGTTCTTT TGGCGGGATT 4801 TTTCGGATTT TTGTCGCGTT CACACACACA CACCAGTTGG TGTTGCCTGT CGTCACCCAC 4861 CCGCCCCACT CTTTGTCCGT GTACTGCTTT GCCATTTGTG CGCGCGTACC GCTGCCCGTC 4921 TGTCTTTCTC ATCATATGCG CCTCGTTTTG TCGCTTTTTC TCCATTTGCC CTTCCTACAT 4981 GGGTACCTGC GGTTTTCATC GAAAATATCC TTAAAAATTC CGAAAAACCT CTATTTTTCG 5041 ATTTTTGAAT CGATTTTCGC CCAAACATAA CCATTTTTCA GAataaaCCT AGAGGATCCC 5101 CGGGATTGGC CAAAGGACCC AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT 5161 TTTCAGGAGG ACCCTTGGCT AGCGTCGACG GTACCGGTCA GATATCACTC GAGGAGCTCC 5221 CGAGATCTTC AGCGCTCAGC ATTCGTAGAA TTgtaagttt aaactttctc atactaacta 5281 acgcgctcta tttaaatttt cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT 5341 TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta 5401 ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG 5461 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 5521 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 5581 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 5641 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 5701 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 5761 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 5821 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 5881 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 5941 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 6001 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 6061 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 6121 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 6181 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 6241 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 6301 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 6361 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 6421 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 6481 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 6541 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 6601 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 6661 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 6721 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 6781 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 6841 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 6901 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 6961 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 7021 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 7081 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 7141 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 7201 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 7261 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 7321 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 7381 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 7441 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 7501 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 7561 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 7621 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 7681 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 7741 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 7801 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 7861 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 7921 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 7981 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 8041 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 8101 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 8161 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 8221 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 8281 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 8341 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 8401 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 8461 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 8521 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 8581 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 8641 AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt 8701 ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA 8761 ACAACTTGGA AATGAAAT // 1611 LOCUS ADDGENE_1611 8473 bp DNA circular SYN 01/03/2005 DEFINITION L3928 complete plasmid sequence KEYWORDS . SOURCE L3928 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1611 at www.addgene.org FEATURES Location/Qualifiers source 1..8473 /organism="Addgene plasmid L3928" /mol_type="other DNA" misc_feature 34..59 /label="mex3.-5064" misc_feature 5074..5099 /label="mex3.Tsa" misc_feature 5142..5183 /label="SynIVS.A" misc_feature 5190..5207 /label="rf21.28" promoter 6387..6415 /label="Amp_promoter" gene 6457..7317 /label="Ampicillin" /gene="Ampicillin" CDS 6457..7317 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7472..8091 /label="pBR322_origin" misc_feature 8307..8333 /label="lacI.C" promoter 8400..8429 /label="lac_promoter" misc_feature 8464..8482 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGATGGAAT TGGTGACCTA GAAAATATTT 61 TTTTGAAATT TCTGCTCCAC CAATTTCAAT TATAGCATAA CTCATTTCAT AGTTGTAGGA 121 CGGTGATTGA AATTTAGGAT AGACAGATTT TAAACCAAAA CAGATTGATG GCCCTTGAAA 181 TTTCTAGGCC ACCTCTCCAG AAAAAAATCT GAAAATTGTT TTTATTTCTA ATTCATTTTC 241 TTCGTCCGTT TTTCATCTCT CGAGGATCTT ATCCCCTTGT CTTACGTCTC CCACCCGCTG 301 CTATTTGGGC GACCTTCATA ACTGTGAAAA TTCTCACCAT TCTCCCTCTC TCGTTTTTGC 361 CCCCCCCCCC CGGTCTCCTA TCATTGTTCA AGTACACCTG TTGTCCCATA ACATCTGCCA 421 ACCAATTTTT TCCAATTTTG CCACAATTTT TATTCAAAAA AGTGCATTGC AGCGGCAGGC 481 ATGCCTCACT CGTACACACA CTTGAGCATA CAGGAAAAGC GAAAAAATCA GTGATTGTTT 541 GGATTCCTGG CTCTTTCTAG GAAAAAAACT CTTCCATTTT TGTAGTGCAC ATCAAAATGA 601 TCAAGCTGGT TAATTTTCAA GTGACTCCCA AAAAAATAAC TGAGAAAGTT GCGTTTTAAA 661 GTTTCTAGGC CATTGTGGGA TTTCTAGGTA GGTTTTTGAA AATAGAAGAC AGTCTTGCAC 721 TAAAGTTTTG ATTAGGCTGT TAAATGATTT TTGTCAGTAC GTTTTTTGGA ACTTCGATTT 781 TTGCCAAAAA TTCCTTTTTT AGTTTTTGTT TCAGTTTTTT GCAAATTTTT ATTTCTTCCA 841 TATCTTACAA CATTTAGAAG GTCTCAAAGT CTCTGATAGC CAAGAATTGA CTCTAATGAC 901 GTTATGAGCT CCGAGACTAT TTTAACCTTT TTCCCTTTTA AGCATAAAAT ATATAAATAT 961 CAAAAATTTA ATACAAAAAC TTCTCGTTTC GAAACGTTTC GAAATCCTCC AGTTAAAAAA 1021 AAATTAGATT ACGGCATCGG AAAAATTGCG AAACTTTTGA ATCTGAGGGA TTTTCAATAG 1081 AGGGTCCCGC CAGGAATTTG AGTTTTTAAC AGTACCCTTT CCATCGTCCT TCCCGAAGCC 1141 CATTACATCA TCATTAATTT TGCGACCTTT CTAAAAGCGG TCATGGGGGG GGGGACATGT 1201 GTTTGTTGCA TATACAGGGA CCCCCCGCCA CAACCACCGC AACTAGAGAT AAAGATCTGC 1261 TGCACACAGA CAGCTGGCAC ATGGATGGAC AGAATGTCTC CTTAATTTTA CCGCCTATTT 1321 CATAATAATA AAAGTAGAAC GGGGGGCAGA AAAGAGAATG ATTGCGAAAC CACCTGCTTG 1381 GCTGTCAGCG CATAAACTTT TCGGAAATTT TGGGTCCCAC CACGCTTTTT TCGGTAACAA 1441 ATTTTGAGAT TTCTCGATAC ACCATGATGT CTTTTGGTGG AATTTTGGGT TATCATGGCT 1501 AATACAAAGC TTTAACGTGC CTTGAACTTT CGCAATTTGA ACTTTTGGCC TTTACTTAAA 1561 AATTTCGATG GGGCTCTATA AGCCATGTGG TTTCGAACTT TTCCTGAAAA TTTAGATTTT 1621 TGTTCATGCA CCATGTTGTT TTTTTTTTGG AATAATTGAG GAATTCCTTA CTTTTAAAGT 1681 AAAAAGTTTG GTGAGATCCA TGTGTTTTCA TATTATTCAG TTGGTAAATT ATACAGATTA 1741 TTGACTGAAG GTTAATTTTT TGTTGGGAAA ATAAAAATCG CCAAAATCAA TAAAATCGTA 1801 TTTAGAAGTA GGGTTTCCAT ATAGGCGGTA AAAGGCTTGT CTGCCTGACA ATAAGACGAC 1861 CGCCGCTCGC CTCAATCCGC GGCGGAGGCA GGCAGGGTTC AGGTAGGCAC TGAAACCGCG 1921 CCTGCTTACC ATGATGTTCC GTTCTTGTCG ATGCACCATG ATAATTTTGG GTTACATTTT 1981 GAGCCTTTTC GAAAAATTAA TGGTGGAAAA TTTCAAGCTG AATTTTAATA TTTATTTTTT 2041 TAACCGTATT GTGGGACATG CAAATGTCTT TCATTTTGTG TCCCTTCACT CTAACGTCTT 2101 CAGAATTTTC GAACTTCTCG ATGCACCATA ATGCTGTTTG GCGATAACTT TAGAGTAAGA 2161 ATGGTAGCGA TTTTTGCGAT GCACCATGAC GTTTTTATCG ATTTTCAGAA AAAAAATCAC 2221 TCCGAGACCC AAATAGTTTC ATTCTCGACG CACTATAACG TTTTCGAAGT AAATTTCAAA 2281 ATTGCTTCTC GGAAAAGTTT TTTGAGACCT ACATGCACCT TTAATCTTCT TTGGAAACTT 2341 TGGAATTTCC GCCGAATACT CTCATTTCAG TTGTTCTCGA TGCACCATGA TGGTATTTTT 2401 TTCAAATTAC TTTTTTTTGA TTTTTCCATG AGAGGACCAA TATCTGTCTT TATAACGGCG 2461 CATTCTGTAA CCAAATTTTG AAATTCCACG ACATTCTGCT CTCATCGATT CAACATGATA 2521 ATTTTAAAAT GAAATTTAGC GATTTTTCGG GAAAAAAACA GTTTCGAGAT TTTCTAGTTA 2581 TTGGACACTC TGCACCTTGC TTATTTGGAA ATTACATTTT AAACCGACAA CCCTTCAAAA 2641 ACTGCTTTTC GTCGATGCAC TATGAGTGCA TAATTTTGTG TAAATCGTGG TGGGCCTCTA 2701 AAAAAACCGA AAAAAAAAAC GTATAAAACG CAAACAAATT GTTTTTGTTG TAATGTTGAC 2761 ACCAACAGAT TCGGCGTGGA ATATACGGAA GATCGGATTT CCAAAAAAAC AACCGCTCTC 2821 CTGTTTTCGA CGTTCCGCCG CTTTTTATGG GAGAAGTAGT ATGTACCTTT AAGGGTACTG 2881 TAGCCCCTTG AATATTGGCC TAAAAGTGAA ACTAGTGTTG CCAAAAAAAA ACGAGATGAC 2941 CAGTTTCAAT ATGTCATTTA CATCTACACA TTTCTAAAAT CTCATTTTAA ATTCCATTGT 3001 TTTCTCGTTT CTTCTGTCCC AAAGTACCGT ATCCTCATCC TTCTACCACC TTAATTTGCA 3061 TTTCTTCTTC CTTATTCACT CTTCTTCCCC TGGACGAAAA ATGTTGCACG ACAACAACAA 3121 AAGAGACCCG CCCCCCACCA TCGGTTTCTC TGAACCTCTA ACTGTCCCCG CCCCCTTTTG 3181 GGAGCCCACT GTCTTTTCTT TTTTTCTTTG GTCGCTGATT ATGACGGATC GTTTTTGTCG 3241 TTTTTGTATT ACAGGAATAC AAATTTCCCA AAACGCGTAT TGCACAACAT ATTTGACGTG 3301 CAAAATATCT AGTAGCGAAA ACTATAGTAA CTCTTTAAAT GACTTTTGTA GCACTGATGC 3361 CGATTTAATT AAATTATTTA TTAACAGATA AAATATTAAA AGAAAACACG ACAATTCAAA 3421 AAAATTCATT TCGTAAATCG AGCCCGTAAA TCGACACAAG CGCTACAGTA GTCATTTAAA 3481 GAATTACTGT TTTTTCGCTA CGAGATATTT TGTGCGTCAA ATATGTTGCG CAATACGCAT 3541 TCTTATAATT TTGTGTACCC GTAGTATATT TTTGTCTCTC TGATTTCCAT TGCAATTTTT 3601 TAATTCCATA TTTTTTTGGT GACAAATTTC TCCGCTTATC AATTTTTTTT TCAACCACAT 3661 AATTATTAAC CCCAAAACAT CGCAGATTTT CAGGTAAAAT GCCAGTCGTG AGCGTTAGAC 3721 CTTTTTCTAT GAGAAATGGT GAGCAATTTT TTTGAAGTTT TTTTTTGAAA TTTTTAATTT 3781 TTCCAGAAGG CTTCTCAAAC GGGCATAACA CGTGGAACGA CGCCGAATTT TGGAAACCGT 3841 GAGTTTTCGG CAAATAAGCT AACAGTCTAA GAAACACGAA AAAAAATCCA ATTTTCTTTT 3901 CAAAATTCGT GATTTTCGGA TGATTTTTGA ACTTTTTGAT TAATGTTCCC ACTGAAAATC 3961 CTCCAAATCT CTAAAAATCC GCTTCTAAAA AAGCCTCAAA TGGCCTTGCG AGAATAGACG 4021 GAATGTTCAA GGATGACTGG TATAAAATCG GCAAACGTGT TGCCATTCAA ACAGAATATC 4081 TCGAAATTTC GCCTTCCTTT TTTTTAAGTG GGTCGGATAT TTAACATACA GAAGAGAATA 4141 GGGAGAGAAA AGAGGGAAAA GAGGGGAAAA GTCTTCTTGG AAACAACCTC GAACCGCAAT 4201 TAAGTTTTTG CCTCACCCTT CAATTTTCGT GAGAAAGTTA AAAATCCAAA TTTTTTTCTC 4261 CTTTTTTAAA AAAAGATTTC CTCCTCAAAA ATTCAAAGGA TCGAATTTTT TGTTACACGC 4321 CTGCCTCCTT TTTATTACCC TTGTTCTTGA TCGCAACCTC TTTTCTAGAT TACTTGAAAA 4381 AATGCGCTAT TTTTTTTCCT TTTTCAACAG AGTCAATTTC AAAGGTGCTG TGGAAAAAGT 4441 TTCGGTTTTT TTTTGCAAAA ATAATCGAAA TTTGTTCGTT TATACTGCTT AAAAAATGAC 4501 CAAAATTTCA AGTAATTCAT TCGAAAAATC GATTTTTTTG AAATATACAA AAATTTTCAA 4561 TTTTTCATAT CTGAAAACAC AATTTTTTCG ATTTTTCATT TTTCTTGGAA AAAACACAAA 4621 AAAAAACTGT ATTTAATTTC CCACTTAATA CTACGATATC CAAGCCCCGC CTCCGCGTAT 4681 TGTACGTGAA CACGGCGGCC CAAAATTACA CACTATCTCA CACACGTTAA ATGAGTGATA 4741 CCGTACTCTC TCTGTTTTTT CTTTCATTTT TTCGGCGCCG AAAATTTTCA ATTTTTGGTG 4801 GTTCTTTTGG CGGGATTTTT CGGATTTTTG TCGCGTTCAC ACACACACAC CAGTTGGTGT 4861 TGCCTGTCGT CACCCACCCG CCCCACTCTT TGTCCGTGTA CTGCTTTGCC ATTTGTGCGC 4921 GCGTACCGCT GCCCGTCTGT CTTTCTCATC ATATGCGCCT CGTTTTGTCG CTTTTTCTCC 4981 ATTTGCCCTT CCTACATGGG TACCTGCGGT TTTCATCGAA AATATCCTTA AAAATTCCGA 5041 AAAACCTCTA TTTTTCGATT TTTGAATCGA TTTTCGCCCA AACATAACCA TTTTTCAGAa 5101 taaaCCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT 5161 AACATAACAT AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCATGGTATT 5221 GATATCTGAG CTCCGCATCG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG 5281 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 5341 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 5401 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 5461 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 5521 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 5581 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 5641 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 5701 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 5761 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 5821 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 5881 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 5941 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 6001 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 6061 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 6121 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 6181 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 6241 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 6301 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 6361 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 6421 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 6481 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 6541 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 6601 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 6661 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 6721 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 6781 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 6841 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 6901 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 6961 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 7021 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 7081 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 7141 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 7201 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 7261 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 7321 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 7381 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 7441 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 7501 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 7561 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 7621 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 7681 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 7741 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 7801 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 7861 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 7921 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 7981 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 8041 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 8101 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 8161 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 8221 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 8281 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 8341 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 8401 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 8461 ACAGGAAACA GCT // 1612 LOCUS ADDGENE_1612 9110 bp DNA circular SYN 01/03/2005 DEFINITION L3929 complete plasmid sequence KEYWORDS . SOURCE L3929 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1612 at www.addgene.org FEATURES Location/Qualifiers source 1..9110 /organism="Addgene plasmid L3929" /mol_type="other DNA" misc_feature 17..42 /label="mex3.-5064" misc_feature 5057..5082 /label="mex3.Tsa" promoter complement(5131..5149) /label="T7_promoter" CDS 5166..6074 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 5166..6014 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 5337..5387 /label="SynIVS.R" misc_feature 5409..5428 /label="gfp.S65C" misc_feature 5542..5592 /label="SynIVS.S" misc_feature 5757..5807 /label="SynIVS.T" misc_feature 6180..6209 /label="let858.3'" promoter 6890..6918 /label="Amp_promoter" CDS 6960..7820 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6960..7820 /label="Ampicillin" /gene="Ampicillin" rep_origin 7975..8594 /label="pBR322_origin" misc_feature 8810..8836 /label="lacI.C" promoter 8903..8932 /label="lac_promoter" misc_feature 8967..8985 /label="M13r_primer" misc_feature 8999..9049 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGATGG AATTGGTGAC CTAGAAAATA TTTTTTTGAA ATTTCTGCTC 61 CACCAATTTC AATTATAGCA TAACTCATTT CATAGTTGTA GGACGGTGAT TGAAATTTAG 121 GATAGACAGA TTTTAAACCA AAACAGATTG ATGGCCCTTG AAATTTCTAG GCCACCTCTC 181 CAGAAAAAAA TCTGAAAATT GTTTTTATTT CTAATTCATT TTCTTCGTCC GTTTTTCATC 241 TCTCGAGGAT CTTATCCCCT TGTCTTACGT CTCCCACCCG CTGCTATTTG GGCGACCTTC 301 ATAACTGTGA AAATTCTCAC CATTCTCCCT CTCTCGTTTT TGCCCCCCCC CCCCGGTCTC 361 CTATCATTGT TCAAGTACAC CTGTTGTCCC ATAACATCTG CCAACCAATT TTTTCCAATT 421 TTGCCACAAT TTTTATTCAA AAAAGTGCAT TGCAGCGGCA GGCATGCCTC ACTCGTACAC 481 ACACTTGAGC ATACAGGAAA AGCGAAAAAA TCAGTGATTG TTTGGATTCC TGGCTCTTTC 541 TAGGAAAAAA ACTCTTCCAT TTTTGTAGTG CACATCAAAA TGATCAAGCT GGTTAATTTT 601 CAAGTGACTC CCAAAAAAAT AACTGAGAAA GTTGCGTTTT AAAGTTTCTA GGCCATTGTG 661 GGATTTCTAG GTAGGTTTTT GAAAATAGAA GACAGTCTTG CACTAAAGTT TTGATTAGGC 721 TGTTAAATGA TTTTTGTCAG TACGTTTTTT GGAACTTCGA TTTTTGCCAA AAATTCCTTT 781 TTTAGTTTTT GTTTCAGTTT TTTGCAAATT TTTATTTCTT CCATATCTTA CAACATTTAG 841 AAGGTCTCAA AGTCTCTGAT AGCCAAGAAT TGACTCTAAT GACGTTATGA GCTCCGAGAC 901 TATTTTAACC TTTTTCCCTT TTAAGCATAA AATATATAAA TATCAAAAAT TTAATACAAA 961 AACTTCTCGT TTCGAAACGT TTCGAAATCC TCCAGTTAAA AAAAAATTAG ATTACGGCAT 1021 CGGAAAAATT GCGAAACTTT TGAATCTGAG GGATTTTCAA TAGAGGGTCC CGCCAGGAAT 1081 TTGAGTTTTT AACAGTACCC TTTCCATCGT CCTTCCCGAA GCCCATTACA TCATCATTAA 1141 TTTTGCGACC TTTCTAAAAG CGGTCATGGG GGGGGGGACA TGTGTTTGTT GCATATACAG 1201 GGACCCCCCG CCACAACCAC CGCAACTAGA GATAAAGATC TGCTGCACAC AGACAGCTGG 1261 CACATGGATG GACAGAATGT CTCCTTAATT TTACCGCCTA TTTCATAATA ATAAAAGTAG 1321 AACGGGGGGC AGAAAAGAGA ATGATTGCGA AACCACCTGC TTGGCTGTCA GCGCATAAAC 1381 TTTTCGGAAA TTTTGGGTCC CACCACGCTT TTTTCGGTAA CAAATTTTGA GATTTCTCGA 1441 TACACCATGA TGTCTTTTGG TGGAATTTTG GGTTATCATG GCTAATACAA AGCTTTAACG 1501 TGCCTTGAAC TTTCGCAATT TGAACTTTTG GCCTTTACTT AAAAATTTCG ATGGGGCTCT 1561 ATAAGCCATG TGGTTTCGAA CTTTTCCTGA AAATTTAGAT TTTTGTTCAT GCACCATGTT 1621 GTTTTTTTTT TGGAATAATT GAGGAATTCC TTACTTTTAA AGTAAAAAGT TTGGTGAGAT 1681 CCATGTGTTT TCATATTATT CAGTTGGTAA ATTATACAGA TTATTGACTG AAGGTTAATT 1741 TTTTGTTGGG AAAATAAAAA TCGCCAAAAT CAATAAAATC GTATTTAGAA GTAGGGTTTC 1801 CATATAGGCG GTAAAAGGCT TGTCTGCCTG ACAATAAGAC GACCGCCGCT CGCCTCAATC 1861 CGCGGCGGAG GCAGGCAGGG TTCAGGTAGG CACTGAAACC GCGCCTGCTT ACCATGATGT 1921 TCCGTTCTTG TCGATGCACC ATGATAATTT TGGGTTACAT TTTGAGCCTT TTCGAAAAAT 1981 TAATGGTGGA AAATTTCAAG CTGAATTTTA ATATTTATTT TTTTAACCGT ATTGTGGGAC 2041 ATGCAAATGT CTTTCATTTT GTGTCCCTTC ACTCTAACGT CTTCAGAATT TTCGAACTTC 2101 TCGATGCACC ATAATGCTGT TTGGCGATAA CTTTAGAGTA AGAATGGTAG CGATTTTTGC 2161 GATGCACCAT GACGTTTTTA TCGATTTTCA GAAAAAAAAT CACTCCGAGA CCCAAATAGT 2221 TTCATTCTCG ACGCACTATA ACGTTTTCGA AGTAAATTTC AAAATTGCTT CTCGGAAAAG 2281 TTTTTTGAGA CCTACATGCA CCTTTAATCT TCTTTGGAAA CTTTGGAATT TCCGCCGAAT 2341 ACTCTCATTT CAGTTGTTCT CGATGCACCA TGATGGTATT TTTTTCAAAT TACTTTTTTT 2401 TGATTTTTCC ATGAGAGGAC CAATATCTGT CTTTATAACG GCGCATTCTG TAACCAAATT 2461 TTGAAATTCC ACGACATTCT GCTCTCATCG ATTCAACATG ATAATTTTAA AATGAAATTT 2521 AGCGATTTTT CGGGAAAAAA ACAGTTTCGA GATTTTCTAG TTATTGGACA CTCTGCACCT 2581 TGCTTATTTG GAAATTACAT TTTAAACCGA CAACCCTTCA AAAACTGCTT TTCGTCGATG 2641 CACTATGAGT GCATAATTTT GTGTAAATCG TGGTGGGCCT CTAAAAAAAC CGAAAAAAAA 2701 AACGTATAAA ACGCAAACAA ATTGTTTTTG TTGTAATGTT GACACCAACA GATTCGGCGT 2761 GGAATATACG GAAGATCGGA TTTCCAAAAA AACAACCGCT CTCCTGTTTT CGACGTTCCG 2821 CCGCTTTTTA TGGGAGAAGT AGTATGTACC TTTAAGGGTA CTGTAGCCCC TTGAATATTG 2881 GCCTAAAAGT GAAACTAGTG TTGCCAAAAA AAAACGAGAT GACCAGTTTC AATATGTCAT 2941 TTACATCTAC ACATTTCTAA AATCTCATTT TAAATTCCAT TGTTTTCTCG TTTCTTCTGT 3001 CCCAAAGTAC CGTATCCTCA TCCTTCTACC ACCTTAATTT GCATTTCTTC TTCCTTATTC 3061 ACTCTTCTTC CCCTGGACGA AAAATGTTGC ACGACAACAA CAAAAGAGAC CCGCCCCCCA 3121 CCATCGGTTT CTCTGAACCT CTAACTGTCC CCGCCCCCTT TTGGGAGCCC ACTGTCTTTT 3181 CTTTTTTTCT TTGGTCGCTG ATTATGACGG ATCGTTTTTG TCGTTTTTGT ATTACAGGAA 3241 TACAAATTTC CCAAAACGCG TATTGCACAA CATATTTGAC GTGCAAAATA TCTAGTAGCG 3301 AAAACTATAG TAACTCTTTA AATGACTTTT GTAGCACTGA TGCCGATTTA ATTAAATTAT 3361 TTATTAACAG ATAAAATATT AAAAGAAAAC ACGACAATTC AAAAAAATTC ATTTCGTAAA 3421 TCGAGCCCGT AAATCGACAC AAGCGCTACA GTAGTCATTT AAAGAATTAC TGTTTTTTCG 3481 CTACGAGATA TTTTGTGCGT CAAATATGTT GCGCAATACG CATTCTTATA ATTTTGTGTA 3541 CCCGTAGTAT ATTTTTGTCT CTCTGATTTC CATTGCAATT TTTTAATTCC ATATTTTTTT 3601 GGTGACAAAT TTCTCCGCTT ATCAATTTTT TTTTCAACCA CATAATTATT AACCCCAAAA 3661 CATCGCAGAT TTTCAGGTAA AATGCCAGTC GTGAGCGTTA GACCTTTTTC TATGAGAAAT 3721 GGTGAGCAAT TTTTTTGAAG TTTTTTTTTG AAATTTTTAA TTTTTCCAGA AGGCTTCTCA 3781 AACGGGCATA ACACGTGGAA CGACGCCGAA TTTTGGAAAC CGTGAGTTTT CGGCAAATAA 3841 GCTAACAGTC TAAGAAACAC GAAAAAAAAT CCAATTTTCT TTTCAAAATT CGTGATTTTC 3901 GGATGATTTT TGAACTTTTT GATTAATGTT CCCACTGAAA ATCCTCCAAA TCTCTAAAAA 3961 TCCGCTTCTA AAAAAGCCTC AAATGGCCTT GCGAGAATAG ACGGAATGTT CAAGGATGAC 4021 TGGTATAAAA TCGGCAAACG TGTTGCCATT CAAACAGAAT ATCTCGAAAT TTCGCCTTCC 4081 TTTTTTTTAA GTGGGTCGGA TATTTAACAT ACAGAAGAGA ATAGGGAGAG AAAAGAGGGA 4141 AAAGAGGGGA AAAGTCTTCT TGGAAACAAC CTCGAACCGC AATTAAGTTT TTGCCTCACC 4201 CTTCAATTTT CGTGAGAAAG TTAAAAATCC AAATTTTTTT CTCCTTTTTT AAAAAAAGAT 4261 TTCCTCCTCA AAAATTCAAA GGATCGAATT TTTTGTTACA CGCCTGCCTC CTTTTTATTA 4321 CCCTTGTTCT TGATCGCAAC CTCTTTTCTA GATTACTTGA AAAAATGCGC TATTTTTTTT 4381 CCTTTTTCAA CAGAGTCAAT TTCAAAGGTG CTGTGGAAAA AGTTTCGGTT TTTTTTTGCA 4441 AAAATAATCG AAATTTGTTC GTTTATACTG CTTAAAAAAT GACCAAAATT TCAAGTAATT 4501 CATTCGAAAA ATCGATTTTT TTGAAATATA CAAAAATTTT CAATTTTTCA TATCTGAAAA 4561 CACAATTTTT TCGATTTTTC ATTTTTCTTG GAAAAAACAC AAAAAAAAAC TGTATTTAAT 4621 TTCCCACTTA ATACTACGAT ATCCAAGCCC CGCCTCCGCG TATTGTACGT GAACACGGCG 4681 GCCCAAAATT ACACACTATC TCACACACGT TAAATGAGTG ATACCGTACT CTCTCTGTTT 4741 TTTCTTTCAT TTTTTCGGCG CCGAAAATTT TCAATTTTTG GTGGTTCTTT TGGCGGGATT 4801 TTTCGGATTT TTGTCGCGTT CACACACACA CACCAGTTGG TGTTGCCTGT CGTCACCCAC 4861 CCGCCCCACT CTTTGTCCGT GTACTGCTTT GCCATTTGTG CGCGCGTACC GCTGCCCGTC 4921 TGTCTTTCTC ATCATATGCG CCTCGTTTTG TCGCTTTTTC TCCATTTGCC CTTCCTACAT 4981 GGGTACCTGC GGTTTTCATC GAAAATATCC TTAAAAATTC CGAAAAACCT CTATTTTTCG 5041 ATTTTTGAAT CGATTTTCGC CCAAACATAA CCATTTTTCA GAataaaCCT AGAGGATCCT 5101 ATCGATTCGC GGCCGCTGTA CACCCGGGTG CCCTATAGTG AGTCGTATTG GTACCGGTAG 5161 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 5221 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 5281 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 5341 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 5401 CTACTTTCTg TTATGGTGTT CAATGCTTcT CGAGATACCC AGATCATATG AAACAGCATG 5461 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 5521 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 5581 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 5641 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 5701 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 5761 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 5821 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 5881 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 5941 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 6001 GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC 6061 GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA 6121 CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT 6181 TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg tctcccaatt 6241 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat 6301 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat 6361 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa 6421 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt 6481 aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 6541 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 6601 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 6661 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 6721 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 6781 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 6841 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 6901 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 6961 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 7021 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 7081 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 7141 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 7201 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 7261 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 7321 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 7381 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 7441 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 7501 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 7561 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 7621 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 7681 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 7741 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 7801 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 7861 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 7921 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 7981 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 8041 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 8101 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 8161 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 8221 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 8281 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 8341 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 8401 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 8461 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 8521 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 8581 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 8641 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 8701 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 8761 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 8821 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 8881 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 8941 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 9001 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 9061 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1613 LOCUS ADDGENE_1613 12566 bp DNA circular SYN 01/03/2005 DEFINITION L3930 complete plasmid sequence KEYWORDS . SOURCE L3930 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1613 at www.addgene.org FEATURES Location/Qualifiers source 1..12566 /organism="Addgene plasmid L3930" /mol_type="other DNA" misc_feature 17..42 /label="mex3.-5064" misc_feature 5057..5082 /label="mex3.Tsa" promoter complement(5131..5149) /label="T7_promoter" gene 5166..6014 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 5166..6074 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 5337..5387 /label="SynIVS.R" misc_feature 5409..5428 /label="gfp.S65C" misc_feature 5542..5592 /label="SynIVS.S" misc_feature 5757..5807 /label="SynIVS.T" misc_feature 6072..6125 /label="let858.smgUTR" CDS 8750..9454 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 9430..9454 /label="let858.C" misc_feature 9532..9561 /label="let858.3'" promoter 10346..10374 /label="Amp_promoter" CDS 10416..11276 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 10416..11276 /label="Ampicillin" /gene="Ampicillin" rep_origin 11431..12050 /label="pBR322_origin" misc_feature 12266..12292 /label="lacI.C" promoter 12359..12388 /label="lac_promoter" misc_feature 12423..12441 /label="M13r_primer" misc_feature 12455..12505 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGATGG AATTGGTGAC CTAGAAAATA TTTTTTTGAA ATTTCTGCTC 61 CACCAATTTC AATTATAGCA TAACTCATTT CATAGTTGTA GGACGGTGAT TGAAATTTAG 121 GATAGACAGA TTTTAAACCA AAACAGATTG ATGGCCCTTG AAATTTCTAG GCCACCTCTC 181 CAGAAAAAAA TCTGAAAATT GTTTTTATTT CTAATTCATT TTCTTCGTCC GTTTTTCATC 241 TCTCGAGGAT CTTATCCCCT TGTCTTACGT CTCCCACCCG CTGCTATTTG GGCGACCTTC 301 ATAACTGTGA AAATTCTCAC CATTCTCCCT CTCTCGTTTT TGCCCCCCCC CCCCGGTCTC 361 CTATCATTGT TCAAGTACAC CTGTTGTCCC ATAACATCTG CCAACCAATT TTTTCCAATT 421 TTGCCACAAT TTTTATTCAA AAAAGTGCAT TGCAGCGGCA GGCATGCCTC ACTCGTACAC 481 ACACTTGAGC ATACAGGAAA AGCGAAAAAA TCAGTGATTG TTTGGATTCC TGGCTCTTTC 541 TAGGAAAAAA ACTCTTCCAT TTTTGTAGTG CACATCAAAA TGATCAAGCT GGTTAATTTT 601 CAAGTGACTC CCAAAAAAAT AACTGAGAAA GTTGCGTTTT AAAGTTTCTA GGCCATTGTG 661 GGATTTCTAG GTAGGTTTTT GAAAATAGAA GACAGTCTTG CACTAAAGTT TTGATTAGGC 721 TGTTAAATGA TTTTTGTCAG TACGTTTTTT GGAACTTCGA TTTTTGCCAA AAATTCCTTT 781 TTTAGTTTTT GTTTCAGTTT TTTGCAAATT TTTATTTCTT CCATATCTTA CAACATTTAG 841 AAGGTCTCAA AGTCTCTGAT AGCCAAGAAT TGACTCTAAT GACGTTATGA GCTCCGAGAC 901 TATTTTAACC TTTTTCCCTT TTAAGCATAA AATATATAAA TATCAAAAAT TTAATACAAA 961 AACTTCTCGT TTCGAAACGT TTCGAAATCC TCCAGTTAAA AAAAAATTAG ATTACGGCAT 1021 CGGAAAAATT GCGAAACTTT TGAATCTGAG GGATTTTCAA TAGAGGGTCC CGCCAGGAAT 1081 TTGAGTTTTT AACAGTACCC TTTCCATCGT CCTTCCCGAA GCCCATTACA TCATCATTAA 1141 TTTTGCGACC TTTCTAAAAG CGGTCATGGG GGGGGGGACA TGTGTTTGTT GCATATACAG 1201 GGACCCCCCG CCACAACCAC CGCAACTAGA GATAAAGATC TGCTGCACAC AGACAGCTGG 1261 CACATGGATG GACAGAATGT CTCCTTAATT TTACCGCCTA TTTCATAATA ATAAAAGTAG 1321 AACGGGGGGC AGAAAAGAGA ATGATTGCGA AACCACCTGC TTGGCTGTCA GCGCATAAAC 1381 TTTTCGGAAA TTTTGGGTCC CACCACGCTT TTTTCGGTAA CAAATTTTGA GATTTCTCGA 1441 TACACCATGA TGTCTTTTGG TGGAATTTTG GGTTATCATG GCTAATACAA AGCTTTAACG 1501 TGCCTTGAAC TTTCGCAATT TGAACTTTTG GCCTTTACTT AAAAATTTCG ATGGGGCTCT 1561 ATAAGCCATG TGGTTTCGAA CTTTTCCTGA AAATTTAGAT TTTTGTTCAT GCACCATGTT 1621 GTTTTTTTTT TGGAATAATT GAGGAATTCC TTACTTTTAA AGTAAAAAGT TTGGTGAGAT 1681 CCATGTGTTT TCATATTATT CAGTTGGTAA ATTATACAGA TTATTGACTG AAGGTTAATT 1741 TTTTGTTGGG AAAATAAAAA TCGCCAAAAT CAATAAAATC GTATTTAGAA GTAGGGTTTC 1801 CATATAGGCG GTAAAAGGCT TGTCTGCCTG ACAATAAGAC GACCGCCGCT CGCCTCAATC 1861 CGCGGCGGAG GCAGGCAGGG TTCAGGTAGG CACTGAAACC GCGCCTGCTT ACCATGATGT 1921 TCCGTTCTTG TCGATGCACC ATGATAATTT TGGGTTACAT TTTGAGCCTT TTCGAAAAAT 1981 TAATGGTGGA AAATTTCAAG CTGAATTTTA ATATTTATTT TTTTAACCGT ATTGTGGGAC 2041 ATGCAAATGT CTTTCATTTT GTGTCCCTTC ACTCTAACGT CTTCAGAATT TTCGAACTTC 2101 TCGATGCACC ATAATGCTGT TTGGCGATAA CTTTAGAGTA AGAATGGTAG CGATTTTTGC 2161 GATGCACCAT GACGTTTTTA TCGATTTTCA GAAAAAAAAT CACTCCGAGA CCCAAATAGT 2221 TTCATTCTCG ACGCACTATA ACGTTTTCGA AGTAAATTTC AAAATTGCTT CTCGGAAAAG 2281 TTTTTTGAGA CCTACATGCA CCTTTAATCT TCTTTGGAAA CTTTGGAATT TCCGCCGAAT 2341 ACTCTCATTT CAGTTGTTCT CGATGCACCA TGATGGTATT TTTTTCAAAT TACTTTTTTT 2401 TGATTTTTCC ATGAGAGGAC CAATATCTGT CTTTATAACG GCGCATTCTG TAACCAAATT 2461 TTGAAATTCC ACGACATTCT GCTCTCATCG ATTCAACATG ATAATTTTAA AATGAAATTT 2521 AGCGATTTTT CGGGAAAAAA ACAGTTTCGA GATTTTCTAG TTATTGGACA CTCTGCACCT 2581 TGCTTATTTG GAAATTACAT TTTAAACCGA CAACCCTTCA AAAACTGCTT TTCGTCGATG 2641 CACTATGAGT GCATAATTTT GTGTAAATCG TGGTGGGCCT CTAAAAAAAC CGAAAAAAAA 2701 AACGTATAAA ACGCAAACAA ATTGTTTTTG TTGTAATGTT GACACCAACA GATTCGGCGT 2761 GGAATATACG GAAGATCGGA TTTCCAAAAA AACAACCGCT CTCCTGTTTT CGACGTTCCG 2821 CCGCTTTTTA TGGGAGAAGT AGTATGTACC TTTAAGGGTA CTGTAGCCCC TTGAATATTG 2881 GCCTAAAAGT GAAACTAGTG TTGCCAAAAA AAAACGAGAT GACCAGTTTC AATATGTCAT 2941 TTACATCTAC ACATTTCTAA AATCTCATTT TAAATTCCAT TGTTTTCTCG TTTCTTCTGT 3001 CCCAAAGTAC CGTATCCTCA TCCTTCTACC ACCTTAATTT GCATTTCTTC TTCCTTATTC 3061 ACTCTTCTTC CCCTGGACGA AAAATGTTGC ACGACAACAA CAAAAGAGAC CCGCCCCCCA 3121 CCATCGGTTT CTCTGAACCT CTAACTGTCC CCGCCCCCTT TTGGGAGCCC ACTGTCTTTT 3181 CTTTTTTTCT TTGGTCGCTG ATTATGACGG ATCGTTTTTG TCGTTTTTGT ATTACAGGAA 3241 TACAAATTTC CCAAAACGCG TATTGCACAA CATATTTGAC GTGCAAAATA TCTAGTAGCG 3301 AAAACTATAG TAACTCTTTA AATGACTTTT GTAGCACTGA TGCCGATTTA ATTAAATTAT 3361 TTATTAACAG ATAAAATATT AAAAGAAAAC ACGACAATTC AAAAAAATTC ATTTCGTAAA 3421 TCGAGCCCGT AAATCGACAC AAGCGCTACA GTAGTCATTT AAAGAATTAC TGTTTTTTCG 3481 CTACGAGATA TTTTGTGCGT CAAATATGTT GCGCAATACG CATTCTTATA ATTTTGTGTA 3541 CCCGTAGTAT ATTTTTGTCT CTCTGATTTC CATTGCAATT TTTTAATTCC ATATTTTTTT 3601 GGTGACAAAT TTCTCCGCTT ATCAATTTTT TTTTCAACCA CATAATTATT AACCCCAAAA 3661 CATCGCAGAT TTTCAGGTAA AATGCCAGTC GTGAGCGTTA GACCTTTTTC TATGAGAAAT 3721 GGTGAGCAAT TTTTTTGAAG TTTTTTTTTG AAATTTTTAA TTTTTCCAGA AGGCTTCTCA 3781 AACGGGCATA ACACGTGGAA CGACGCCGAA TTTTGGAAAC CGTGAGTTTT CGGCAAATAA 3841 GCTAACAGTC TAAGAAACAC GAAAAAAAAT CCAATTTTCT TTTCAAAATT CGTGATTTTC 3901 GGATGATTTT TGAACTTTTT GATTAATGTT CCCACTGAAA ATCCTCCAAA TCTCTAAAAA 3961 TCCGCTTCTA AAAAAGCCTC AAATGGCCTT GCGAGAATAG ACGGAATGTT CAAGGATGAC 4021 TGGTATAAAA TCGGCAAACG TGTTGCCATT CAAACAGAAT ATCTCGAAAT TTCGCCTTCC 4081 TTTTTTTTAA GTGGGTCGGA TATTTAACAT ACAGAAGAGA ATAGGGAGAG AAAAGAGGGA 4141 AAAGAGGGGA AAAGTCTTCT TGGAAACAAC CTCGAACCGC AATTAAGTTT TTGCCTCACC 4201 CTTCAATTTT CGTGAGAAAG TTAAAAATCC AAATTTTTTT CTCCTTTTTT AAAAAAAGAT 4261 TTCCTCCTCA AAAATTCAAA GGATCGAATT TTTTGTTACA CGCCTGCCTC CTTTTTATTA 4321 CCCTTGTTCT TGATCGCAAC CTCTTTTCTA GATTACTTGA AAAAATGCGC TATTTTTTTT 4381 CCTTTTTCAA CAGAGTCAAT TTCAAAGGTG CTGTGGAAAA AGTTTCGGTT TTTTTTTGCA 4441 AAAATAATCG AAATTTGTTC GTTTATACTG CTTAAAAAAT GACCAAAATT TCAAGTAATT 4501 CATTCGAAAA ATCGATTTTT TTGAAATATA CAAAAATTTT CAATTTTTCA TATCTGAAAA 4561 CACAATTTTT TCGATTTTTC ATTTTTCTTG GAAAAAACAC AAAAAAAAAC TGTATTTAAT 4621 TTCCCACTTA ATACTACGAT ATCCAAGCCC CGCCTCCGCG TATTGTACGT GAACACGGCG 4681 GCCCAAAATT ACACACTATC TCACACACGT TAAATGAGTG ATACCGTACT CTCTCTGTTT 4741 TTTCTTTCAT TTTTTCGGCG CCGAAAATTT TCAATTTTTG GTGGTTCTTT TGGCGGGATT 4801 TTTCGGATTT TTGTCGCGTT CACACACACA CACCAGTTGG TGTTGCCTGT CGTCACCCAC 4861 CCGCCCCACT CTTTGTCCGT GTACTGCTTT GCCATTTGTG CGCGCGTACC GCTGCCCGTC 4921 TGTCTTTCTC ATCATATGCG CCTCGTTTTG TCGCTTTTTC TCCATTTGCC CTTCCTACAT 4981 GGGTACCTGC GGTTTTCATC GAAAATATCC TTAAAAATTC CGAAAAACCT CTATTTTTCG 5041 ATTTTTGAAT CGATTTTCGC CCAAACATAA CCATTTTTCA GAataaaCCT AGAGGATCCT 5101 ATCGATTCGC GGCCGCTGTA CACCCGGGTG CCCTATAGTG AGTCGTATTG GTACCGGTAG 5161 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 5221 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 5281 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 5341 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 5401 CTACTTTCTg TTATGGTGTT CAATGCTTcT CGAGATACCC AGATCATATG AAACAGCATG 5461 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 5521 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 5581 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 5641 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 5701 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 5761 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 5821 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 5881 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 5941 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 6001 GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC 6061 GGCCATACAA GTAATccggg ttctagaagc gctgctaagg gggcccTCGT CGAGTCGGTC 6121 ACAATCACCT GAAACTCCAA AGGCAGCCAG TGAGGAACGT GAAGAAGAAG AAAAAGAGTC 6181 ATCTGAACAG gtttgatttt ctttctggtc aaaaagatga aattattgat tttcagCCAG 6241 ATACTCCCAA AACTAGCAGC GAGAAGTCTG CAAGTCGTTC ACAGTCGCCC AGAGAATCGC 6301 GGGAAGTGAG CCAAGAGgta tgtttttcaa aaatcaataa ctgatcataa tttttattgt 6361 ttggtgaatt taagaaaata atattcgaaa attcctctga attatcaaga ttgcagtatt 6421 aatttcgaga aaaattgaga tattcataga gctattgtaa attttcttga tttcagACTG 6481 AAACTTCGGA AAATCAAGAG AAAATCAAAG AAAAGGATGA CGGGGATGAT CAGCCTGGCA 6541 CACCGAACAG CTATAGAAGC CGGGAAACTT CACCAGCTCC AAAAAGGTCC AAGGAGACCA 6601 Ggtttgtcaa aagcttcctg cgattaattc tcatttcaat ttttcagAGA ATCAGAGTCT 6661 CCTGAAAAAT CCCCGGTTCG TTCAAGATCT CCCAGAAGGT CTTCAGCACG TTCCCCGTCA 6721 CGATCTCCTA GACGGCGCCG AGAAAGAAGC TCAGAAAGAA AGCAATCCGA AGAGCCAGCA 6781 CCGCTACCAG AGAAAAAGAA GAAAGAGCCG CTGGATATTC TACGAACAAG AACCGGAGGA 6841 GCATATATTC CACCCGCCAA ACTTCGACTT ATGCAACAAC AGATTAGTGA TAAGCAAAGT 6901 GAACAGTATC AGAGAATGAA TTGGGAAAGA ATGAAGAAAA AGATTCACGG ATTGGTTAAC 6961 AGAGTCAACG CGAAGAATCT TGTTCAAATT GTCAGAGAAC TTCTTCAAGA GAATGTGATT 7021 CGTTCAAAgt gagtgagaaa atcgaaggaa aaggaaagaa ttaatttaat ttttcagGGG 7081 ACTTCTCTGC CGTGACATTA TTCAAGCTCA GGCTTTCTCA CCAGGATTCT CTAACGTCTA 7141 TGCAGCTTTG GCGGCAGTTA TCAACTCGAA ATTCCCTCAT GTCGGTGAAC TTCTTCTCCG 7201 TCGTCTGATT GTACAGTTCA AAAGAAGTTT CCGTAGAAAT GACAGAGGCG TCACGGTGAA 7261 CGTGATCAAA TTCATCGCAC ATTTGATTAA TCAACAAGTT GCTCACGAAG TTCTTGCGCT 7321 GGAAATCATG ATTCTGATGC TTGAAGAACC AACTGATGAT TCAGTTGAAG TCGCCATTGC 7381 GTTCCTGAAA GAGTGTGGAG CAAAGCTTCT GGAGATTGCT CCAGCAGCTC TTAACAGTGT 7441 CTACGACCGT CTTCGTGCAA TTCTCATGGA AACTGAAAGA TCGGAAAATG CACTGGATCG 7501 ACGTATTCAG TATATGATTG AGACTGCAAT GCAGATTCGA AAGGACAAAT TTGCGGCGTA 7561 TCCGGCGGTC ATTGAAGACT TGGACTTGAT TGAGGAGGAG GATCAGATCA TCCATACACT 7621 TAATTTGGAG GATGCGGTTG ATCCGGAAAA TGGGCTTAAT GTGTTCAAAC TAGATCCAGA 7681 ATTCGAAAAG AACGAGGAGG TTTATGAGGA GATCCGTAAG GAAATCATTG GAAACGCCGA 7741 TATTTCGGAT GAGGATGGTG GCGACGAGTT GGATGATGAA GAAGAGGGTA GTGATGTGGA 7801 AGAGGCTCCG AAGAAGACTA CAGAGATTAT TGATAATACT GATCAGAATT GACTGCTTTC 7861 AGAAGgtatt cattttgagt tttgggccgg caaatctgta agttgccggt tgccgaaaat 7921 ttgctgaatt tgccggaaaa aaaaattccg gaatttattt aaaaactttt tgtaaaaatt 7981 aaattaaatt tgcaactttt cagAGAAGTC TACCTGACAA TGCAATCATC TTTGGACTAC 8041 CAAGAAGCTG CTCACAAATT GCTGAAAATG AAGATTCCAG ACAGCATGCA Ggtcagcgat 8101 gttgcaaaga aaaattttcg accaaaaaaa ccaaccaatc ataaaattta aaaaaaaact 8161 ccgttttttt cttttttttt atacgagaaa aaccaaaaaa atgtattttt gccaaattct 8221 aaaatactat ccccgaaatt ttcaatattt tctctttcag AACGAACTCT GCGCGATGCT 8281 TGTCGATTGT TGTGCTCAAC AGCGTACCTA CGAGCGATTC TACGGAATGC TCATCGAACG 8341 TTTCTGCCGA CTTCGCCTCG AATACCAGCA ATACTTTGAA AAGCTCTGCC AGGACACGTA 8401 TTCCACGATT CACCGAATTG ACATCACAAA ACTGCGGAAT TTGGCTCGCC TTATTGCTCA 8461 TTTGCTCTCG ACGGATGCTA TTGACTGGAA GATTTTGGCC GATATGAAAA TGACCGAAGA 8521 GGACACAACT TCTTCTGGCA GAATCTATAT TAAATATATA TTTAATGAAC TTGTGGAGGC 8581 GATGGGAATG GTTAAACTTC ATTCGAGAGT TACTGATCCg tgagtttcct agagagagtt 8641 gttttcgtat tcaattttcc ctattttcag AACTTTGGCT CATTGCTTTG TTGGATTATT 8701 CCCACGAACT AATCCGAACA GCGCACGATT TTCGATCAAC TTCTTCACAA TGATTGGATT 8761 GGGTGGTTTG ACGTTGGAAC TTCGTGAATG GCTGGCAAAG GGTCTCAAGA AGAAGAAGGG 8821 AATGCTGGAT CAGTTGAAGG CCGAATCAAG CTCAGATTCA TCGTCGTCTT CGGATTCGTC 8881 AGACTCGTCT GATTCTTCGG ATTCTGACGA TTCATCCGAC TCGTCTTCAG ATTCCTCATC 8941 TTCTTCAGAA TCAGAGCCAG AACCACCGAA GAAAAAGAAG AAGAAGAACA GTGAAGAGAG 9001 TTCCAAAAAG AAGGAAAAAG AGAATATTGG TCGACGGGAT CGTGGAGACA AGAGAGCTGA 9061 ACGTCATCGT GATCAAAGTG TGGAGAACAA GGACAAGGAT CGTCGACGTC GCCAGGATTC 9121 TGACGAAAAT CGTCGGCCAG AACGAGGAGA TGACCGCAAG GATCGGAGTA AAGATCGTCG 9181 TCGTCAAGAC TCGGATGATG AGGATCGGAA AGGTCGTGAA CGTCGGGAAG ATTCAGGGGA 9241 AAGACGTCGC GGAGATCGGG ATCGACGTGA TCGAAACAAG GATCAGGAGG ATCACCGTGA 9301 AGATCGCCGT GACCGAAGCA AGGATCGTGA GGATCGACGT GATCGCCGTC GTCATGACTC 9361 TGATGATGAT CGTAAAACTC GTCGGGATAG AAGTGAAGAG CGAGGAGGAC GTCGTCGTGA 9421 AGTGGAATCG GATGATCGAC GCCGACGTCG TTGAATTTTC AAATTTTAAA TACTGAATAT 9481 TTGTTTTTTT TCCTATTATT TATTTATTCT CTTTGTGTTT TTTTTCTTGC TTTCTAAAAA 9541 ATTAATTCAA TCCAAATCTA Aacatttttt tttctctttc cgtctcccaa ttcgtattcc 9601 gctcctctca tctgaacaca atgtgcaagt ttatttatct tctcgctttc atttcattag 9661 gacgtggggg gaattggtgg aagggggaaa cacacaaaag gatgatggaa atgaaataag 9721 gacacacaat atgcaacaac attcaattca gaaatatgga ggaaggttta aaagaaaaca 9781 taaaaatata tagaggagga aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac 9841 gatgagaatt gtcctcgctt ggcaaatgcg aatccgtatg gagaggcacg tttggcgaag 9901 gcaaatgttc ggtatggaga tctgtaaaaa tttttaagtt gaaatttggt gttgctcttt 9961 tacaaaattt tccgattttc gcttgaaatt acggtgccag gtctcgacac gtcttccaat 10021 ttttcaaatt caaaagagcc tttaatgggc tgtagttgct aatttctcgt ttttgaaaat 10081 ttttcttccg tttaatcgaa atttgatgta ttttatttat gattttcaat aaatttcaaa 10141 gaaactggtg aaaactcgga aaattgtgaa ctacagtaat ccaatcctta aaggcgcaca 10201 ccttttaaat gtccgcccca atacgatatt tttttaagat tcgCTAGAGC GGCCCAAAAG 10261 GCCTTTTTTT TTTGGCGCCG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 10321 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 10381 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 10441 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 10501 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 10561 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 10621 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 10681 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 10741 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 10801 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 10861 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 10921 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 10981 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 11041 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 11101 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 11161 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 11221 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 11281 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 11341 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 11401 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 11461 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 11521 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 11581 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 11641 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 11701 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 11761 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 11821 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 11881 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 11941 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 12001 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 12061 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 12121 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 12181 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 12241 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 12301 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 12361 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 12421 CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt ttaaacatga tcttactaac 12481 taactattct catttaaatt ttcagAGCTT AAAAATGGCT GAAATCACTC ACAACGATGG 12541 ATACGCTAAC AACTTGGAAA TGAAAT // 1614 LOCUS ADDGENE_1614 4712 bp DNA circular SYN 01/03/2005 DEFINITION L4018 complete plasmid sequence KEYWORDS . SOURCE L4018 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1614 at www.addgene.org FEATURES Location/Qualifiers source 1..4712 /organism="Addgene plasmid L4018" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" misc_feature 136..162 /label="SV40.NLS" CDS 136..1173 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 178..204 /label="SV40.NLS" misc_feature 220..246 /label="SV40.NLS" misc_feature 262..288 /label="SV40.NLS" gene 304..1164 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 475..525 /label="SynIVS.R" misc_feature 547..566 /label="gfp.S65C" misc_feature 680..730 /label="SynIVS.S" misc_feature 895..945 /label="SynIVS.T" misc_feature 1187..1237 /label="SynIVS.M" misc_feature 1314..1364 /label="SynIVS.L" promoter 2492..2520 /label="Amp_promoter" gene 2562..3422 /label="Ampicillin" /gene="Ampicillin" CDS 2562..3422 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3577..4196 /label="pBR322_origin" misc_feature 4412..4438 /label="lacI.C" promoter 4505..4534 /label="lac_promoter" misc_feature 4569..4587 /label="M13r_primer" misc_feature 4601..4651 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGC AGAAAAAATG 181 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG 241 CGTAAGGTAC CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGTAGAA 301 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 361 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 421 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 481 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 541 ACTTTCTgTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA ACgGCATGAC 601 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 661 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 721 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 781 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 841 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 901 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 961 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 1021 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 1081 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 1141 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTgtaa gtttaaactt 1201 tctcatacta actaacgcgc tctatttaaa ttttcagAAT TCCAACTGAG CGCCGGTCGC 1261 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1321 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1381 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1441 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1501 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1561 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1621 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1681 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1741 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1801 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1861 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1921 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 1981 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2041 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2101 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2161 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2221 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2281 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2341 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2401 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2461 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2521 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2581 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2641 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2701 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2761 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2821 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2881 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 2941 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3001 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3061 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3121 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3181 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3241 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3301 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3361 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3421 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3481 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3541 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3601 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3661 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3721 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3781 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3841 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3901 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 3961 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4021 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4081 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4141 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4201 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4261 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4321 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4381 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4441 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4501 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4561 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 4621 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 4681 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1615 LOCUS ADDGENE_1615 4717 bp DNA circular SYN 01/03/2005 DEFINITION L4019 complete plasmid sequence KEYWORDS . SOURCE L4019 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1615 at www.addgene.org FEATURES Location/Qualifiers source 1..4717 /organism="Addgene plasmid L4019" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" misc_feature 141..167 /label="SV40.NLS" CDS 141..1178 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 183..209 /label="SV40.NLS" misc_feature 225..251 /label="SV40.NLS" misc_feature 267..293 /label="SV40.NLS" gene 309..1169 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 480..530 /label="SynIVS.R" misc_feature 552..571 /label="gfp.S65C" misc_feature 685..735 /label="SynIVS.S" misc_feature 900..950 /label="SynIVS.T" misc_feature 1192..1242 /label="SynIVS.M" misc_feature 1319..1369 /label="SynIVS.L" promoter 2497..2525 /label="Amp_promoter" gene 2567..3427 /label="Ampicillin" /gene="Ampicillin" CDS 2567..3427 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3582..4201 /label="pBR322_origin" misc_feature 4417..4443 /label="lacI.C" promoter 4510..4539 /label="lac_promoter" misc_feature 4574..4592 /label="M13r_primer" misc_feature 4606..4656 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGCAGAAA 181 AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGCAGA AAAAATGACT GCTCCAAAGA 241 AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 301 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 481 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 541 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 661 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 721 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 781 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 841 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 901 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 961 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1021 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1081 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1141 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT Tgtaagttta 1201 aactttctca tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG 1261 GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt 1321 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC 1381 CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG 1441 TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA 1501 GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG 1561 TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA 1621 ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA 1681 ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC 1741 CGCAATTTTT ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG 1801 AAAAAGTTTT GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT 1861 TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT 1921 TTCTTGCTAA AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG 1981 GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA 2041 TGGGGTTTTT GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT 2101 ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 2161 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 2221 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 2281 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC 2341 GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA 2401 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT 2461 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 2521 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 2581 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 2641 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 2701 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 2761 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 2821 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 2881 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 2941 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 3001 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 3061 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 3121 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 3181 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 3241 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 3301 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 3361 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 3421 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 3481 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 3541 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3601 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3661 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3721 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3781 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3841 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3901 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3961 CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG 4021 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 4081 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4141 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4201 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT 4261 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG 4321 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 4381 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 4441 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 4501 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 4561 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg 4621 atcttactaa ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT 4681 CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1616 LOCUS ADDGENE_1616 4711 bp DNA circular SYN 01/03/2005 DEFINITION L4020 complete plasmid sequence KEYWORDS . SOURCE L4020 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1616 at www.addgene.org FEATURES Location/Qualifiers source 1..4711 /organism="Addgene plasmid L4020" /mol_type="other DNA" CDS 9..1172 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPVEKMSKG EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNI YILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD GNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN YNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQN TPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" misc_feature 177..203 /label="SV40.NLS" misc_feature 219..245 /label="SV40.NLS" misc_feature 261..287 /label="SV40.NLS" gene 303..1163 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 474..524 /label="SynIVS.R" misc_feature 546..565 /label="gfp.S65C" misc_feature 679..729 /label="SynIVS.S" misc_feature 894..944 /label="SynIVS.T" misc_feature 1186..1236 /label="SynIVS.M" misc_feature 1313..1363 /label="SynIVS.L" promoter 2491..2519 /label="Amp_promoter" CDS 2561..3421 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2561..3421 /label="Ampicillin" /gene="Ampicillin" rep_origin 3576..4195 /label="pBR322_origin" misc_feature 4411..4437 /label="lacI.C" promoter 4504..4533 /label="lac_promoter" misc_feature 4568..4586 /label="M13r_primer" misc_feature 4600..4650 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGCA GAAAAAATGA 181 CTGCTCCAAA GAAGAAGCGT AAGGTACCGG CAGAAAAAAT GACTGCTCCA AAGAAGAAGC 241 GTAAGGTACC GGCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 301 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 361 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 421 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 481 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 541 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 601 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 661 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 721 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 781 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 841 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 901 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 961 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 1021 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 1081 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1141 TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt 1201 ctcatactaa ctaacgcgct ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT 1261 ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt 1321 aaactgagtt ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC 1381 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 1441 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 1501 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 1561 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 1621 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 1681 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 1741 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 1801 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 1861 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 1921 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 1981 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 2041 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 2101 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 2161 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 2221 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 2281 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 2341 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 2401 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 2461 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 2521 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 2581 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 2641 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 2701 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 2761 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 2821 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 2881 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 2941 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 3001 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 3061 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 3121 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 3181 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 3241 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 3301 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 3361 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 3421 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 3481 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 3541 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3601 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3661 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3721 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3781 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3841 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3901 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3961 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4021 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4081 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4141 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4201 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 4261 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 4321 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 4381 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 4441 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 4501 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 4561 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta 4621 ctaactaact attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC 4681 GATGGATACG CTAACAACTT GGAAATGAAA T // 1617 LOCUS ADDGENE_1617 6322 bp DNA circular SYN 01/03/2005 DEFINITION L4026 complete plasmid sequence KEYWORDS . SOURCE L4026 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1617 at www.addgene.org FEATURES Location/Qualifiers source 1..6322 /organism="Addgene plasmid L4026" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" misc_feature 1746..1772 /label="SV40.NLS" CDS 1746..2783 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 1788..1814 /label="SV40.NLS" misc_feature 1830..1856 /label="SV40.NLS" misc_feature 1872..1898 /label="SV40.NLS" gene 1914..2774 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2085..2135 /label="SynIVS.R" misc_feature 2157..2176 /label="gfp.S65C" misc_feature 2290..2340 /label="SynIVS.S" misc_feature 2505..2555 /label="SynIVS.T" misc_feature 2797..2847 /label="SynIVS.M" misc_feature 2924..2974 /label="SynIVS.L" promoter 4102..4130 /label="Amp_promoter" gene 4172..5032 /label="Ampicillin" /gene="Ampicillin" CDS 4172..5032 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5187..5806 /label="pBR322_origin" misc_feature 6022..6048 /label="lacI.C" promoter 6115..6144 /label="lac_promoter" misc_feature 6179..6197 /label="M13r_primer" misc_feature 6211..6261 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGC AGAAAAAATG ACTGCTCCAA 1801 AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC 1861 CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGTAGAA AAAATGAGTA 1921 AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA 1981 ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA 2041 CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt ttaaacatat 2101 atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT ACTTTCTgTT 2161 ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA ACgGCATGAC TTTTTCAAGA 2221 GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT GACGGGAACT 2281 ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag 2341 GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA AAAGGTATTG 2401 ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT AACTCACACA 2461 ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt ttaaacatga 2521 ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA CACAACATTG 2581 AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC 2641 CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA 2701 ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG 2761 GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTgtaa gtttaaactt tctcatacta 2821 actaacgcgc tctatttaaa ttttcagAAT TCCAACTGAG CGCCGGTCGC TACCATTACC 2881 AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt taaactgagt 2941 tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC CTCTGACTTC 3001 TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA 3061 TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA 3121 CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA 3181 AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC 3241 TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA 3301 CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC 3361 TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT 3421 ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT 3481 TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA 3541 CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT 3601 GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT 3661 AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC 3721 GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 3781 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 3841 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 3901 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 3961 CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 4021 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 4081 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 4141 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 4201 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 4261 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 4321 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 4381 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 4441 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 4501 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 4561 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 4621 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 4681 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 4741 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 4801 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 4861 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 4921 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 4981 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 5041 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 5101 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 5161 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 5221 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 5281 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 5341 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 5401 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 5461 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 5521 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 5581 CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 5641 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 5701 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 5761 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 5821 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 5881 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 5941 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 6001 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 6061 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 6121 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 6181 AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt actaactaac 6241 tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA CGATGGATAC 6301 GCTAACAACT TGGAAATGAA AT // 1618 LOCUS ADDGENE_1618 5128 bp DNA circular SYN 01/03/2005 DEFINITION L4035 complete plasmid sequence KEYWORDS . SOURCE L4035 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1618 at www.addgene.org FEATURES Location/Qualifiers source 1..5128 /organism="Addgene plasmid L4035" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" CDS 514..1644 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTPDNATVWVRKHKDCP PPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 514..544 /label="pat3.MbLS" gene 775..1635 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 946..996 /label="SynIVS.R" misc_feature 1018..1037 /label="gfp.S65C" misc_feature 1151..1201 /label="SynIVS.S" misc_feature 1366..1416 /label="SynIVS.T" misc_feature 1730..1780 /label="SynIVS.L" promoter 2908..2936 /label="Amp_promoter" gene 2978..3838 /label="Ampicillin" /gene="Ampicillin" CDS 2978..3838 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3993..4612 /label="pBR322_origin" misc_feature 4828..4854 /label="lacI.C" promoter 4921..4950 /label="lac_promoter" misc_feature 4985..5003 /label="M13r_primer" misc_feature 5017..5067 /label="decoy" ORIGIN 1 AAGCTTGcat gCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGccg gcaatgccac cttcaacatc attgctgctc 541 ctcgcagcac ttcttccatt cgctttacca gcaagcgatt ggaagactgg agaagtcact 601 ccggataatg caacagtctg ggtcagaaaa cataaagatt gtcctccacc tgtccctgtg 661 ctcgcaattg tgctcggagt cattgcgggt atcgtaatcc tcggaattct tctcttgttg 721 ctctggaaat tgctcacagt acttcatgat agatccgagg taccGGTAGA AAAAATGAGT 781 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT 841 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT 901 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag tttaaacata 961 tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC TACTTTCTgT 1021 TATGGTGTTC AATGCTTcTC gAGATACCCA GATCATATGA AACgGCATGA CTTTTTCAAG 1081 AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA TGACGGGAAC 1141 TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt taaattttca 1201 gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT AAAAGGTATT 1261 GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA TAACTCACAC 1321 AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag tttaaacatg 1381 attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG ACACAACATT 1441 GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT TGGCGATGGC 1501 CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC 1561 AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG GATTACACAT 1621 GGCATGGATG AACTATACAA ATAGCATTCG TAGAATTCCA ACTGAGCGCC GGTCGCTACC 1681 ATTACCAACT TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa 1741 ctgagttcta ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT 1801 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 1861 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 1921 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 1981 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 2041 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 2101 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 2161 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 2221 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 2281 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 2341 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 2401 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 2461 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 2521 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 2581 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 2641 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 2701 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 2761 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 2821 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 2881 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 2941 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 3001 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 3061 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 3121 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 3181 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 3241 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 3301 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 3361 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 3421 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 3481 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 3541 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 3601 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 3661 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 3721 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 3781 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 3841 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 3901 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 3961 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4021 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4081 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4141 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4201 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4261 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4321 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4381 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4441 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4501 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4561 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4621 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 4681 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 4741 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 4801 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 4861 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 4921 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 4981 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 5041 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 5101 GGATACGCTA ACAACTTGGA AATGAAAT // 1619 LOCUS ADDGENE_1619 6637 bp DNA circular SYN 01/03/2005 DEFINITION L4037 complete plasmid sequence KEYWORDS . SOURCE L4037 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1619 at www.addgene.org FEATURES Location/Qualifiers source 1..6637 /organism="Addgene plasmid L4037" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2450..2491 /label="SynIVS.A" CDS 2525..3601 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 2525..2551 /label="SV40.NLS" misc_feature 2567..2593 /label="SV40.NLS" misc_feature 2609..2635 /label="SV40.NLS" misc_feature 2651..2677 /label="SV40.NLS" gene 2693..3541 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2864..2914 /label="SynIVS.R" misc_feature 2936..2955 /label="gfp.S65C" misc_feature 3069..3119 /label="SynIVS.S" misc_feature 3284..3334 /label="SynIVS.T" misc_feature 3707..3736 /label="let858.3'" promoter 4417..4445 /label="Amp_promoter" gene 4487..5347 /label="Ampicillin" /gene="Ampicillin" CDS 4487..5347 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5502..6121 /label="pBR322_origin" misc_feature 6337..6363 /label="lacI.C" promoter 6430..6459 /label="lac_promoter" misc_feature 6494..6512 /label="M13r_primer" misc_feature 6526..6576 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCCCAAAG GACCCAAAGG TATGTTTCGA 2461 ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGAGGGTAC CGAGCTCAGA 2521 AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA 2581 GAAGAAGCGT AAGGTACCGG CAGAAAAAAT GACTGCTCCA AAGAAGAAGC GTAAGGTACC 2641 GGCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA 2701 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA 2761 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC 2821 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata 2881 tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA 2941 TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG 3001 TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA 3061 CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta aattttcagG 3121 TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA 3181 TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA 3241 TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat 3301 tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA 3361 AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC 3421 TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA 3481 CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG 3541 CATGGAcGAA CTATACAAAa gcccacaAGC TTTGGAATTC GCTAGCCGGC CATACAAGTA 3601 ATCCGGATGA TCGACGCCaA CGTCGTTGAA TTTTCAAATT TTAAATACTG AATATTTGTT 3661 TTTTTTCCTA TTATTTATTT ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT AAAAAATTAA 3721 TTCAATCCAA ATCTAAacat ttttttttct ctttccgtct cccaattcgt attccgctcc 3781 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt 3841 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac 3901 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa 3961 atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatgA 4021 GAATTGTCCT CGCTTGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 4081 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 4141 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 4201 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC 4261 GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA 4321 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT 4381 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 4441 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 4501 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 4561 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 4621 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 4681 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 4741 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 4801 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 4861 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 4921 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 4981 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 5041 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 5101 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 5161 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 5221 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 5281 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 5341 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 5401 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 5461 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 5521 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 5581 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 5641 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 5701 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 5761 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 5821 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 5881 CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG 5941 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 6001 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 6061 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 6121 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT 6181 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG 6241 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 6301 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 6361 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 6421 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 6481 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg 6541 atcttactaa ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT 6601 CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1620 LOCUS ADDGENE_1620 7732 bp DNA circular SYN 01/03/2005 DEFINITION L4038 complete plasmid sequence KEYWORDS . SOURCE L4038 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1620 at www.addgene.org FEATURES Location/Qualifiers source 1..7732 /organism="Addgene plasmid L4038" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" misc_feature 3545..3586 /label="SynIVS.A" misc_feature 3620..3646 /label="SV40.NLS" CDS 3620..4696 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 3662..3688 /label="SV40.NLS" misc_feature 3704..3730 /label="SV40.NLS" misc_feature 3746..3772 /label="SV40.NLS" gene 3788..4636 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3959..4009 /label="SynIVS.R" misc_feature 4031..4050 /label="gfp.S65C" misc_feature 4164..4214 /label="SynIVS.S" misc_feature 4379..4429 /label="SynIVS.T" misc_feature 4802..4831 /label="let858.3'" promoter 5512..5540 /label="Amp_promoter" CDS 5582..6442 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5582..6442 /label="Ampicillin" /gene="Ampicillin" rep_origin 6597..7216 /label="pBR322_origin" misc_feature 7432..7458 /label="lacI.C" promoter 7525..7554 /label="lac_promoter" misc_feature 7589..7607 /label="M13r_primer" misc_feature 7621..7671 /label="decoy" misc_feature 7729..7752 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtggtacc gctagccgta 3481 cgaacccggg ttCTAGAGGA TCCTATCGAT TCGCGGCCGC TGTACACCCC CAAAGGACCC 3541 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGAG 3601 GGTACCGAGC TCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGCAGAAAA 3661 AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA AAAATGACTG CTCCAAAGAA 3721 GAAGCGTAAG GTACCGGCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT 3781 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 3841 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 3901 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 3961 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 4021 CACTACTTTC TgTTATGGTG TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA 4081 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 4141 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 4201 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 4261 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 4321 CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt 4381 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 4441 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 4501 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT 4561 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 4621 TGGGATTACA CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCGCTAG 4681 CCGGCCATAC AAGTAATCCG GATGATCGAC GCCaACGTCG TTGAATTTTC AAATTTTAAA 4741 TACTGAATAT TTGTTTTTTT TCCTATTATT TATTTATTCT CTTTGTGTTT TTTTTCTTGC 4801 TTTCTAAAAA ATTAATTCAA TCCAAATCTA Aacatttttt tttctctttc cgtctcccaa 4861 ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct tctcgctttc 4921 atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag gatgatggaa 4981 atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga ggaaggttta 5041 aaagaaaaca taaaaatata tagaggagga aggaaaacta gtaaaaaata agcaaagaaa 5101 ttaggcgaac gatgAGAATT GTCCTCGCTT GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT 5161 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG 5221 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC 5281 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA 5341 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT 5401 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC 5461 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT 5521 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG 5581 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC 5641 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 5701 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC 5761 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC 5821 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT 5881 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT 5941 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT 6001 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT 6061 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT 6121 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC 6181 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 6241 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 6301 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA 6361 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC 6421 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA 6481 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 6541 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 6601 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 6661 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 6721 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT 6781 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 6841 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 6901 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 6961 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC 7021 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 7081 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 7141 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 7201 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 7261 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 7321 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA 7381 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 7441 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 7501 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 7561 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT 7621 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gAGCTTAAAA 7681 ATGGCTGAAA TCACTCACAA CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1621 LOCUS ADDGENE_1621 5537 bp DNA circular SYN 01/03/2005 DEFINITION L4040 complete plasmid sequence KEYWORDS . SOURCE L4040 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1621 at www.addgene.org FEATURES Location/Qualifiers source 1..5537 /organism="Addgene plasmid L4040" /mol_type="other DNA" misc_feature 20..44 /label="myo2.-1090" misc_feature 1108..1131 /label="myo2.5'" misc_feature 1205..1246 /label="SynIVS.A" misc_feature 1253..1270 /label="rf21.28" misc_feature 1350..1391 /label="SynIVS.A" CDS 1425..2501 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 1425..1451 /label="SV40.NLS" misc_feature 1467..1493 /label="SV40.NLS" misc_feature 1509..1535 /label="SV40.NLS" misc_feature 1551..1577 /label="SV40.NLS" gene 1593..2441 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1764..1814 /label="SynIVS.R" misc_feature 1836..1855 /label="gfp.S65C" misc_feature 1969..2019 /label="SynIVS.S" misc_feature 2184..2234 /label="SynIVS.T" misc_feature 2607..2636 /label="let858.3'" promoter 3317..3345 /label="Amp_promoter" CDS 3387..4247 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3387..4247 /label="Ampicillin" /gene="Ampicillin" rep_origin 4402..5021 /label="pBR322_origin" misc_feature 5237..5263 /label="lacI.C" promoter 5330..5359 /label="lac_promoter" misc_feature 5394..5412 /label="M13r_primer" misc_feature 5426..5476 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGAGGCATTT GAATTGGGGG TGGTGGACAG TAACTGTCTG 61 TAATAATAAT TACTCCTGAC CAGGTTGCAA TTCGAGTTTT GATAAGCATA ATTATACCTT 121 GTACATTGTG GGTTTTGTGC TGTGGACGTT TTATTGTGAC ATCCCCATAA GCTACAAGAA 181 CAAAAATGAA ATgTAAAAGT ATTGAAAACG TCGTACATTT TATATCTGAG TAGTATCCTT 241 TGCTTTAAAT GTCCATAAAA cTAATTTTAT AATCAATAAA ACAACGTTTG TAAATCAACT 301 GAGTTTACAA GTAGAGACAT TGAGGGATAC TTTCACTATG CTAAAGTGAT AATCGACCAA 361 ATAATAACTt CACTTTGGTA TTTATTCCTG TCTTATAAaT GTTATGTATG AATTAAATTC 421 ATATGCATAT GGCTCACTCT GACAAttttt AATAATCTTC CAGATCAATA TTGACTACCG 481 ATGCGGGTGG TCTTTTGCTT TGAATTCTGC TGAACTTTAC ACCCCGAACA GCAATGTGTG 541 CTTCAGCcTA AAAAAAAGTA AGTGTGTTAA TCAGTgCCCC CGATTCTTCA TTTTTTGCCC 601 CTCTCTCCCG TTTCGTCGGC AAAAGAAGAG AAAATAAAGA TAAGTCTCAA GATAGGTTGG 661 TAATCGCTAA AGTGGTTGTG TGGATAAGAG TAGCAAAATG GCAGGAAGAG CACTTTGCGC 721 GCACACACTG TACTCATTGT TCTGGATAAA ATTCTCTCGT TGTTTGCCGT CGGATGTCTG 781 CCTCTCTGCA TTGAGCCGGC TTCTTCACTA TCTTTAGTTA ACCTAAAATG CCGTTTCTTT 841 TCTCGTATCC CcACTATcCC GTTGAGGTTC TCTGCTCTCT TCGCTCCCTA CCGCCAGCGA 901 GCAACTATCC GTGGGGGCGC CTTGCTCGGA AGATGGGGGG GAAGAAAGAA GATTTTTGCT 961 ATTTGCACTT GAGAAAGAGA CTTTTCCTGC GTCGATGGTT AGAGAACAGT GTGCAGACAC 1021 TTTTCAGCTA CCTAGAatTA CAatTGGATA TCCCCGCCTC CCAATCCACC CACCCAGGGA 1081 AAAAGAAGGG CTCGCCGAAA ATCAAAGTTA TCTCCAGGCT CGCGCATCCC ACCGAGCGGT 1141 TGACTTCTCT CCACCACTTT TCATTTTAAC CCTCGGGGTA CGGGATTGGC CAAAGGACCC 1201 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGCT 1261 AGCGTCGACG GTACCGGTCA GATATCACTC GAGGAGCTCC CGAGATCCTA TCGATTCGCG 1321 GCCGCTGTAC ACCCCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA CATAACATAG 1381 AACATTTTCA GGAGGACCCT TGGAGGGTAC CGAGCTCAGA AAAAATGACT GCTCCAAAGA 1441 AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 1501 CAGAAAAAAT GACTGCTCCA AAGAAGAAGC GTAAGGTACC GGCAGAAAAA ATGACTGCTC 1561 CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA CTTTTCACTG 1621 GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA 1681 GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA 1741 CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact aaccctgatt 1801 atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA 1861 GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG 1921 TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt aagtttaaac 1981 agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG 2041 GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA 2101 TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA 2161 AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact aactaatctg 2221 atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG 2281 CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC 2341 ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG 2401 TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGAcGAA CTATACAAAa 2461 gcccacaAGC TTTGGAATTC GCTAGCCGGC CATACAAGTA ATCCGGATGA TCGACGCCaA 2521 CGTCGTTGAA TTTTCAAATT TTAAATACTG AATATTTGTT TTTTTTCCTA TTATTTATTT 2581 ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT AAAAAATTAA TTCAATCCAA ATCTAAacat 2641 ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg 2701 caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg 2761 ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca 2821 attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa 2881 aactagtaaa aaataagcaa agaaattagg cgaacgatgA GAATTGTCCT CGCTTGGGCC 2941 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 3001 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 3061 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 3121 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 3181 CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 3241 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 3301 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 3361 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 3421 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 3481 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 3541 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 3601 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 3661 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 3721 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 3781 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 3841 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 3901 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 3961 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 4021 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 4081 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 4141 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 4201 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 4261 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 4321 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 4381 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 4441 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 4501 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 4561 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 4621 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 4681 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 4741 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 4801 AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 4861 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 4921 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 4981 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 5041 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 5101 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 5161 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 5221 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 5281 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 5341 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 5401 GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa ctaactattc 5461 tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG GATACGCTAA 5521 CAACTTGGAA ATGAAAT // 1622 LOCUS ADDGENE_1622 4280 bp DNA circular SYN 01/03/2005 DEFINITION L4041 complete plasmid sequence KEYWORDS . SOURCE L4041 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1622 at www.addgene.org FEATURES Location/Qualifiers source 1..4280 /organism="Addgene plasmid L4041" /mol_type="other DNA" misc_feature 93..134 /label="SynIVS.A" CDS 168..1244 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 168..194 /label="SV40.NLS" misc_feature 210..236 /label="SV40.NLS" misc_feature 252..278 /label="SV40.NLS" misc_feature 294..320 /label="SV40.NLS" gene 336..1184 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 507..557 /label="SynIVS.R" misc_feature 579..598 /label="gfp.S65C" misc_feature 712..762 /label="SynIVS.S" misc_feature 927..977 /label="SynIVS.T" misc_feature 1350..1379 /label="let858.3'" promoter 2060..2088 /label="Amp_promoter" gene 2130..2990 /label="Ampicillin" /gene="Ampicillin" CDS 2130..2990 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3145..3764 /label="pBR322_origin" misc_feature 3980..4006 /label="lacI.C" promoter 4073..4102 /label="lac_promoter" misc_feature 4137..4155 /label="M13r_primer" misc_feature 4169..4219 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGGATC CTATCGATTC 61 GCGGCCGCTG TACACCCCCA AAGGACCCAA AGGTATGTTT CGAATGATAC TAACATAACA 121 TAGAACATTT TCAGGAGGAC CCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA 181 AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC 241 CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA AAAATGACTG 301 CTCCAAAGAA GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA 361 CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG 421 TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA 481 CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg 541 attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT 601 CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT 661 ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta 721 aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG 781 AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA 841 ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG 901 ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat 961 ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC 1021 TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA 1081 ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA 1141 TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAc GAACTATACA 1201 AAagcccaca AGCTTTGGAA TTCGCTAGCC GGCCATACAA GTAATCCGGA TGATCGACGC 1261 CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA 1321 TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa 1381 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat 1441 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa 1501 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat 1561 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag 1621 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT CCTCGCTTGG 1681 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 1741 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 1801 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT 1861 ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 1921 CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 1981 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 2041 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 2101 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 2161 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 2221 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 2281 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA 2341 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 2401 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 2461 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 2521 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 2581 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 2641 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 2701 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 2761 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 2821 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 2881 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 2941 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 3001 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 3061 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 3121 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 3181 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 3241 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 3301 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 3361 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 3421 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 3481 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 3541 TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 3601 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 3661 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 3721 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 3781 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 3841 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 3901 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 3961 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 4021 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 4081 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA 4141 ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta 4201 ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC 4261 TAACAACTTG GAAATGAAAT // 1623 LOCUS ADDGENE_1623 4674 bp DNA circular SYN 01/03/2005 DEFINITION L4042 complete plasmid sequence KEYWORDS . SOURCE L4042 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1623 at www.addgene.org FEATURES Location/Qualifiers source 1..4674 /organism="Addgene plasmid L4042" /mol_type="other DNA" misc_feature complement(130..154) /label="hsp16-2.5'" misc_feature 368..392 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" CDS 562..1638 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 562..588 /label="SV40.NLS" misc_feature 604..630 /label="SV40.NLS" misc_feature 646..672 /label="SV40.NLS" misc_feature 688..714 /label="SV40.NLS" gene 730..1578 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 901..951 /label="SynIVS.R" misc_feature 973..992 /label="gfp.S65C" misc_feature 1106..1156 /label="SynIVS.S" misc_feature 1321..1371 /label="SynIVS.T" misc_feature 1744..1773 /label="let858.3'" promoter 2454..2482 /label="Amp_promoter" gene 2524..3384 /label="Ampicillin" /gene="Ampicillin" CDS 2524..3384 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3539..4158 /label="pBR322_origin" misc_feature 4374..4400 /label="lacI.C" promoter 4467..4496 /label="lac_promoter" misc_feature 4531..4549 /label="M13r_primer" misc_feature 4563..4613 /label="decoy" ORIGIN 1 AAGCTTGcat gcctgcaggt cgactctaga gGATCACCAA AAACGGAACG TTGAGCTGGA 61 CGGAAATAGT GGTAAAGTGA CATGATTATA GTTTGAAGAT TTCTAATTTC ACAATTAGAG 121 CAAATGTTGT TCGGTATTTA TTTTCAACGG TATTTATACT ATTTTCCACC TTTTTCTAGA 181 ACATTCGAGC TGCTTGTTGC AAAAGGAGGG CGACTCACAT TCGGTACATG GAAAAGTAGT 241 GTACACAATA AAGAGACCCA GATACATTTT CCGTCTGCGT CTCTTTGCAC CCACCGGGAG 301 TATTTTCAAA CGAATGCATC TAGGACCTTC TAGAACATTC TGTAAGGCTG CAGAATGCGG 361 GTATATAAGG AAAGCGGGCT CAGAGGAAGC CAACACGCTT TGTTCTAGTG CATCTAAAAA 421 ACTTCGAAAA Tcctcatcgg GATCCTATCG ATTCGCGGCC GCTGTACACC CCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 AGGGTACCGA GCTCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA 601 AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGCAG AAAAAATGAC TGCTCCAAAG 661 AAGAAGCGTA AGGTACCGGC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG TAAGGTACCG 721 GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA 781 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA 841 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG 901 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gCCAACACTT 961 GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCgAGAT ACCCAGATCA TATGAAACgG 1021 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC 1081 AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa ctaaccatac 1141 atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATAGAATC 1201 GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA ATTGGAATAC 1261 AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT 1321 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gAACTTCAAA 1381 ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA ACAAAATACT 1441 CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC ACAATCTGCC 1501 CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT TGTAACAGCT 1561 GCTGGGATTA CACATGGCAT GGAcGAACTA TACAAAagcc cacaAGCTTT GGAATTCGCT 1621 AGCCGGCCAT ACAAGTAATC CGGATGATCG ACGCCaACGT CGTTGAATTT TCAAATTTTA 1681 AATACTGAAT ATTTGTTTTT TTTCCTATTA TTTATTTATT CTCTTTGTGT TTTTTTTCTT 1741 GCTTTCTAAA AAATTAATTC AATCCAAATC TAAacatttt tttttctctt tccgtctccc 1801 aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt 1861 tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg 1921 aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt 1981 taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga 2041 aattaggcga acgatgAGAA TTGTCCTCGC TTGGGCCCTT TCGTCTCGCG CGTTTCGGTG 2101 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 2161 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 2221 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 2281 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 2341 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 2401 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 2461 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 2521 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 2581 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 2641 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 2701 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 2761 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 2821 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 2881 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 2941 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 3001 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 3061 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 3121 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 3181 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 3241 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 3301 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 3361 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 3421 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 3481 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 3541 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 3601 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 3661 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 3721 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 3781 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 3841 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 3901 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 3961 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 4021 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 4081 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 4141 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 4201 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 4261 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 4321 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 4381 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 4441 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 4501 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 4561 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 4621 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1624 LOCUS ADDGENE_1624 4666 bp DNA circular SYN 01/03/2005 DEFINITION L4043 complete plasmid sequence KEYWORDS . SOURCE L4043 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1624 at www.addgene.org FEATURES Location/Qualifiers source 1..4666 /organism="Addgene plasmid L4043" /mol_type="other DNA" misc_feature complement(115..139) /label="hsp16-41.5'" misc_feature 353..377 /label="hsp16-2.5'" misc_feature 479..520 /label="SynIVS.A" misc_feature 554..580 /label="SV40.NLS" CDS 554..1630 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 596..622 /label="SV40.NLS" misc_feature 638..664 /label="SV40.NLS" misc_feature 680..706 /label="SV40.NLS" gene 722..1570 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 893..943 /label="SynIVS.R" misc_feature 965..984 /label="gfp.S65C" misc_feature 1098..1148 /label="SynIVS.S" misc_feature 1313..1363 /label="SynIVS.T" misc_feature 1736..1765 /label="let858.3'" promoter 2446..2474 /label="Amp_promoter" gene 2516..3376 /label="Ampicillin" /gene="Ampicillin" CDS 2516..3376 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3531..4150 /label="pBR322_origin" misc_feature 4366..4392 /label="lacI.C" promoter 4459..4488 /label="lac_promoter" misc_feature 4523..4541 /label="M13r_primer" misc_feature 4555..4605 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCAAGAG CATTTGAATC AGAATATGGA 61 GAACGGAGCA TGAGCATTTT CGAAGTTTTT TAGATGCACT AGAACAAAGC GTGTTGGCTT 121 CCTCTGAGCC CGCTTTCCTT ATATACCCGC ATTCTGCAGC CTTACAGAAT GTTCTAGAAG 181 GTCCTAGATG CATTCGTTTG AAAATACTCC CGGTGGGTGC AAAGAGACGC AGACGGAAAA 241 TGTATCTGGG TCTCTTTATT GTGTACACTA CTTTTCCATG TACCGAATGT GAGTCGCCCT 301 CCTTTTGCAA CAAGCAGCTC GAATGTTCTA GAAAAAGGTG GAAAATAGTA TAAATACCGT 361 TGAAAATAAA TACCGAACAA CATTTGCTCT AATTGTGAAA TTAGAAATCT TCAAACTATA 421 ATCATctcac tgGATCCTAT CGATTCGCGG CCGCTGTACA CCCCCAAAGG ACCCAAAGGT 481 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 541 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGCAG AAAAAATGAC 601 TGCTCCAAAG AAGAAGCGTA AGGTACCGGC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 661 TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGTAGAAAA 721 AATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG 781 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG 841 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGgtaagttt 901 aaacatatat atactaacta accctgatta tttaaatttt cagCCAACAC TTGTCACTAC 961 TTTCTgTTAT GGTGTTCAAT GCTTcTCgAG ATACCCAGAT CATATGAAAC gGCATGACTT 1021 TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA 1081 CGGGAACTAC AAGACACgta agtttaaaca gttcggtact aactaaccat acatatttaa 1141 attttcagGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA 1201 AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAA 1261 CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT GGAATCAAAG TTgtaagttt 1321 aaacatgatt ttactaacta actaatctga tttaaatttt cagAACTTCA AAATTAGACA 1381 CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG 1441 CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA 1501 AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT 1561 TACACATGGC ATGGAcGAAC TATACAAAag cccacaAGCT TTGGAATTCG CTAGCCGGCC 1621 ATACAAGTAA TCCGGATGAT CGACGCCaAC GTCGTTGAAT TTTCAAATTT TAAATACTGA 1681 ATATTTGTTT TTTTTCCTAT TATTTATTTA TTCTCTTTGT GTTTTTTTTC TTGCTTTCTA 1741 AAAAATTAAT TCAATCCAAA TCTAAacatt tttttttctc tttccgtctc ccaattcgta 1801 ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca 1861 ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa 1921 taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa 1981 aacataaaaa tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc 2041 gaacgatgAG AATTGTCCTC GCTTGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 2101 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 2161 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 2221 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 2281 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 2341 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 2401 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 2461 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 2521 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 2581 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2641 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 2701 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 2761 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 2821 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 2881 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 2941 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 3001 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 3061 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 3121 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 3181 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 3241 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 3301 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 3361 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 3421 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 3481 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3541 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3601 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3661 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 3721 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3781 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3841 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3901 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 3961 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4021 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4081 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 4141 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 4201 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 4261 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 4321 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 4381 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 4441 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 4501 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt 4561 ttaaacatga tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT 4621 GAAATCACTC ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT // 1625 LOCUS ADDGENE_1625 4248 bp DNA circular SYN 01/03/2005 DEFINITION L4046 complete plasmid sequence KEYWORDS . SOURCE L4046 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1625 at www.addgene.org FEATURES Location/Qualifiers source 1..4248 /organism="Addgene plasmid L4046" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" CDS 136..1212 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 136..162 /label="SV40.NLS" misc_feature 178..204 /label="SV40.NLS" misc_feature 220..246 /label="SV40.NLS" misc_feature 262..288 /label="SV40.NLS" gene 304..1152 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 475..525 /label="SynIVS.R" misc_feature 547..566 /label="gfp.S65C" misc_feature 680..730 /label="SynIVS.S" misc_feature 895..945 /label="SynIVS.T" misc_feature 1318..1347 /label="let858.3'" promoter 2028..2056 /label="Amp_promoter" CDS 2098..2958 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2098..2958 /label="Ampicillin" /gene="Ampicillin" rep_origin 3113..3732 /label="pBR322_origin" misc_feature 3948..3974 /label="lacI.C" promoter 4041..4070 /label="lac_promoter" misc_feature 4105..4123 /label="M13r_primer" misc_feature 4137..4187 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGC AGAAAAAATG 181 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG 241 CGTAAGGTAC CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGTAGAA 301 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 361 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 421 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 481 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 541 ACTTTCTgTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA ACgGCATGAC 601 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 661 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 721 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 781 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 841 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 901 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 961 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 1021 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 1081 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 1141 ATTACACATG GCATGGAcGA ACTATACAAA agcccacaAG CTTTGGAATT CGCTAGCCGG 1201 CCATACAAGT AATCCGGATG ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT 1261 GAATATTTGT TTTTTTTCCT ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC 1321 TAAAAAATTA ATTCAATCCA AATCTAAaca tttttttttc tctttccgtc tcccaattcg 1381 tattccgctc ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt 1441 cattaggacg tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga 1501 aataaggaca cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag 1561 aaaacataaa aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag 1621 gcgaacgatg AGAATTGTCC TCGCTTGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG 1681 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 1741 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC 1801 TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC 1861 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC 1921 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 1981 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 2041 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 2101 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 2161 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 2221 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 2281 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 2341 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 2401 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 2461 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 2521 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 2581 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 2641 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 2701 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 2761 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 2821 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 2881 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 2941 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 3001 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 3061 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 3121 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 3181 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 3241 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 3301 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 3361 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 3421 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 3481 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT 3541 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 3601 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 3661 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 3721 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 3781 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 3841 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 3901 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 3961 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC 4021 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT 4081 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa 4141 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG 4201 CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1626 LOCUS ADDGENE_1626 4253 bp DNA circular SYN 01/03/2005 DEFINITION L4047 complete plasmid sequence KEYWORDS . SOURCE L4047 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1626 at www.addgene.org FEATURES Location/Qualifiers source 1..4253 /organism="Addgene plasmid L4047" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 141..1217 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 141..167 /label="SV40.NLS" misc_feature 183..209 /label="SV40.NLS" misc_feature 225..251 /label="SV40.NLS" misc_feature 267..293 /label="SV40.NLS" gene 309..1157 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 480..530 /label="SynIVS.R" misc_feature 552..571 /label="gfp.S65C" misc_feature 685..735 /label="SynIVS.S" misc_feature 900..950 /label="SynIVS.T" misc_feature 1323..1352 /label="let858.3'" promoter 2033..2061 /label="Amp_promoter" CDS 2103..2963 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2103..2963 /label="Ampicillin" /gene="Ampicillin" rep_origin 3118..3737 /label="pBR322_origin" misc_feature 3953..3979 /label="lacI.C" promoter 4046..4075 /label="lac_promoter" misc_feature 4110..4128 /label="M13r_primer" misc_feature 4142..4192 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGCAGAAA 181 AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGCAGA AAAAATGACT GCTCCAAAGA 241 AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 301 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 481 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 541 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 661 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 721 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 781 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 841 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 901 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 961 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1021 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1081 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1141 CTGGGATTAC ACATGGCATG GAcGAACTAT ACAAAagccc acaAGCTTTG GAATTCGCTA 1201 GCCGGCCATA CAAGTAATCC GGATGATCGA CGCCaACGTC GTTGAATTTT CAAATTTTAA 1261 ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC TCTTTGTGTT TTTTTTCTTG 1321 CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt ttttctcttt ccgtctccca 1381 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt 1441 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga 1501 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt 1561 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa 1621 attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCTTT CGTCTCGCGC GTTTCGGTGA 1681 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 1741 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 1801 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA 1861 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA 1921 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 1981 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 2041 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 2101 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 2161 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 2221 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 2281 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 2341 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 2401 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 2461 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 2521 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 2581 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 2641 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 2701 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 2761 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 2821 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 2881 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 2941 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 3001 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 3061 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 3121 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 3181 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 3241 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 3301 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 3361 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 3421 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 3481 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA 3541 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 3601 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 3661 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 3721 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 3781 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG 3841 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 3901 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT 3961 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT 4021 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG 4081 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 4141 Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA 4201 AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1627 LOCUS ADDGENE_1627 4247 bp DNA circular SYN 01/03/2005 DEFINITION L4048 complete plasmid sequence KEYWORDS . SOURCE L4048 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1627 at www.addgene.org FEATURES Location/Qualifiers source 1..4247 /organism="Addgene plasmid L4048" /mol_type="other DNA" CDS 9..1211 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPVEKMSKG EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNI YILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD GNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN YNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQN TPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQAL EFASRPYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" misc_feature 177..203 /label="SV40.NLS" misc_feature 219..245 /label="SV40.NLS" misc_feature 261..287 /label="SV40.NLS" gene 303..1151 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 474..524 /label="SynIVS.R" misc_feature 546..565 /label="gfp.S65C" misc_feature 679..729 /label="SynIVS.S" misc_feature 894..944 /label="SynIVS.T" misc_feature 1317..1346 /label="let858.3'" promoter 2027..2055 /label="Amp_promoter" gene 2097..2957 /label="Ampicillin" /gene="Ampicillin" CDS 2097..2957 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3112..3731 /label="pBR322_origin" misc_feature 3947..3973 /label="lacI.C" promoter 4040..4069 /label="lac_promoter" misc_feature 4104..4122 /label="M13r_primer" misc_feature 4136..4186 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGCA GAAAAAATGA 181 CTGCTCCAAA GAAGAAGCGT AAGGTACCGG CAGAAAAAAT GACTGCTCCA AAGAAGAAGC 241 GTAAGGTACC GGCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 301 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 361 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 421 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 481 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 541 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 601 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 661 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 721 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 781 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 841 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 901 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 961 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 1021 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 1081 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1141 TTACACATGG CATGGAcGAA CTATACAAAa gcccacaAGC TTTGGAATTC GCTAGCCGGC 1201 CATACAAGTA ATCCGGATGA TCGACGCCaA CGTCGTTGAA TTTTCAAATT TTAAATACTG 1261 AATATTTGTT TTTTTTCCTA TTATTTATTT ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT 1321 AAAAAATTAA TTCAATCCAA ATCTAAacat ttttttttct ctttccgtct cccaattcgt 1381 attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc 1441 attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa 1501 ataaggacac acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga 1561 aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg 1621 cgaacgatgA GAATTGTCCT CGCTTGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 1681 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 1741 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 1801 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 1861 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC 1921 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 1981 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 2041 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 2101 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2161 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2221 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2281 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2341 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2401 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2461 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2521 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2581 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2641 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2701 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 2761 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2821 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 2881 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 2941 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 3001 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 3061 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3121 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3181 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3241 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3301 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3361 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3421 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3481 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC 3541 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3601 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3661 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3721 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 3781 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 3841 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 3901 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 3961 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 4021 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 4081 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag 4141 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC 4201 TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1628 LOCUS ADDGENE_1628 4410 bp DNA circular SYN 01/03/2005 DEFINITION L4049 complete plasmid sequence KEYWORDS . SOURCE L4049 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1628 at www.addgene.org FEATURES Location/Qualifiers source 1..4410 /organism="Addgene plasmid L4049" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" CDS 298..1374 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 298..324 /label="SV40.NLS" misc_feature 340..366 /label="SV40.NLS" misc_feature 382..408 /label="SV40.NLS" misc_feature 424..450 /label="SV40.NLS" gene 466..1314 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 637..687 /label="SynIVS.R" misc_feature 709..728 /label="gfp.S65C" misc_feature 842..892 /label="SynIVS.S" misc_feature 1057..1107 /label="SynIVS.T" misc_feature 1480..1509 /label="let858.3'" promoter 2190..2218 /label="Amp_promoter" CDS 2260..3120 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2260..3120 /label="Ampicillin" /gene="Ampicillin" rep_origin 3275..3894 /label="pBR322_origin" misc_feature 4110..4136 /label="lacI.C" promoter 4203..4232 /label="lac_promoter" misc_feature 4267..4285 /label="M13r_primer" misc_feature 4299..4349 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG 361 CGTAAGGTAC CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA 421 AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 481 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 541 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 601 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 661 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 721 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 781 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 841 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 901 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 961 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 1021 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 1081 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 1141 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 1201 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 1261 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAc 1321 GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC GGCCATACAA GTAATCCGGA 1381 TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC 1441 CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC 1501 CAAATCTAAa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc 1561 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga 1621 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat 1681 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata 1741 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT 1801 CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 1861 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 1921 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 1981 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 2041 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 2101 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2161 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 2221 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2281 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 2341 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 2401 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 2461 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 2521 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2581 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 2641 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2701 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 2761 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 2821 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 2881 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 2941 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3001 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3061 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3121 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3181 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 3241 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 3301 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 3361 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 3421 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 3481 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 3541 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 3601 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3661 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3721 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 3781 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 3841 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 3901 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 3961 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4021 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4081 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4141 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4201 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 4261 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 4321 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 4381 ATGGATACGC TAACAACTTG GAAATGAAAT // 1629 LOCUS ADDGENE_1629 4630 bp DNA circular SYN 01/03/2005 DEFINITION L4051 complete plasmid sequence KEYWORDS . SOURCE L4051 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1629 at www.addgene.org FEATURES Location/Qualifiers source 1..4630 /organism="Addgene plasmid L4051" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" CDS 518..1594 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 518..544 /label="SV40.NLS" misc_feature 560..586 /label="SV40.NLS" misc_feature 602..628 /label="SV40.NLS" misc_feature 644..670 /label="SV40.NLS" gene 686..1534 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 857..907 /label="SynIVS.R" misc_feature 929..948 /label="gfp.S65C" misc_feature 1062..1112 /label="SynIVS.S" misc_feature 1277..1327 /label="SynIVS.T" misc_feature 1700..1729 /label="let858.3'" promoter 2410..2438 /label="Amp_promoter" CDS 2480..3340 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2480..3340 /label="Ampicillin" /gene="Ampicillin" rep_origin 3495..4114 /label="pBR322_origin" misc_feature 4330..4356 /label="lacI.C" promoter 4423..4452 /label="lac_promoter" misc_feature 4487..4505 /label="M13r_primer" misc_feature 4519..4569 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGCAGAAAA 601 AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA AAAATGACTG CTCCAAAGAA 661 GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT 721 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA 781 GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA 841 ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa 901 ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC 961 AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA 1021 AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg 1081 tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC 1141 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG 1201 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA 1261 GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa 1321 ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 1381 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 1441 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 1501 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca 1561 AGCTTTGGAA TTCGCTAGCC GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT 1621 GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT 1681 TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt 1741 tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt 1801 atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca 1861 cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga 1921 aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt 1981 aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT 2041 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 2101 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 2161 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 2221 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 2281 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 2341 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2401 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 2461 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 2521 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 2581 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 2641 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 2701 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 2761 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 2821 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 2881 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 2941 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3001 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3061 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3121 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3181 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3241 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3301 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3361 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 3421 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3481 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3541 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3601 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 3661 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3721 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3781 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 3841 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 3901 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 3961 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4021 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4081 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4141 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4201 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4261 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4321 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4381 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 4441 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 4501 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 4561 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 4621 GAAATGAAAT // 1630 LOCUS ADDGENE_1630 4249 bp DNA circular SYN 01/03/2005 DEFINITION L4052 complete plasmid sequence KEYWORDS . SOURCE L4052 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1630 at www.addgene.org FEATURES Location/Qualifiers source 1..4249 /organism="Addgene plasmid L4052" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..1213 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 179..205 /label="SV40.NLS" misc_feature 221..247 /label="SV40.NLS" misc_feature 263..289 /label="SV40.NLS" gene 305..1153 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 476..526 /label="SynIVS.R" misc_feature 548..567 /label="gfp.S65C" misc_feature 681..731 /label="SynIVS.S" misc_feature 896..946 /label="SynIVS.T" misc_feature 1319..1348 /label="let858.3'" promoter 2029..2057 /label="Amp_promoter" gene 2099..2959 /label="Ampicillin" /gene="Ampicillin" CDS 2099..2959 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3114..3733 /label="pBR322_origin" misc_feature 3949..3975 /label="lacI.C" promoter 4042..4071 /label="lac_promoter" misc_feature 4106..4124 /label="M13r_primer" misc_feature 4138..4188 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG CAGAAAAAAT 181 GACTGCTCCA AAGAAGAAGC GTAAGGTACC GGCAGAAAAA ATGACTGCTC CAAAGAAGAA 241 GCGTAAGGTA CCGGCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGTAGA 301 AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA 361 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA 421 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag 481 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC 541 TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA GATCATATGA AACgGCATGA 601 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 661 TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt 721 taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT 781 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA 841 TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag 901 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG 961 ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT 1021 TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC 1081 GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG 1141 GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT TCGCTAGCCG 1201 GCCATACAAG TAATCCGGAT GATCGACGCC aACGTCGTTG AATTTTCAAA TTTTAAATAC 1261 TGAATATTTG TTTTTTTTCC TATTATTTAT TTATTCTCTT TGTGTTTTTT TTCTTGCTTT 1321 CTAAAAAATT AATTCAATCC AAATCTAAac attttttttt ctctttccgt ctcccaattc 1381 gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt 1441 tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg 1501 aaataaggac acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa 1561 gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta 1621 ggcgaacgat gAGAATTGTC CTCGCTTGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 1681 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 1741 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 1801 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 1861 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 1921 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 1981 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 2041 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 2101 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 2161 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 2221 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 2281 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 2341 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 2401 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 2461 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 2521 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 2581 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 2641 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 2701 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 2761 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 2821 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 2881 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 2941 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 3001 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 3061 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3121 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3181 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3241 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 3301 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3361 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3421 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3481 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 3541 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3601 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 3661 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 3721 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 3781 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 3841 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 3901 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 3961 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 4021 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 4081 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTgta 4141 agtttaaaca tgatcttact aactaactat tctcatttaa attttcagAG CTTAAAAATG 4201 GCTGAAATCA CTCACAACGA TGGATACGCT AACAACTTGG AAATGAAAT // 1631 LOCUS ADDGENE_1631 5094 bp DNA circular SYN 01/03/2005 DEFINITION L4053 complete plasmid sequence KEYWORDS . SOURCE L4053 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1631 at www.addgene.org FEATURES Location/Qualifiers source 1..5094 /organism="Addgene plasmid L4053" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" CDS 518..1555 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 518..544 /label="SV40.NLS" misc_feature 560..586 /label="SV40.NLS" misc_feature 602..628 /label="SV40.NLS" misc_feature 644..670 /label="SV40.NLS" gene 686..1546 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 857..907 /label="SynIVS.R" misc_feature 929..948 /label="gfp.S65C" misc_feature 1062..1112 /label="SynIVS.S" misc_feature 1277..1327 /label="SynIVS.T" misc_feature 1569..1619 /label="SynIVS.M" misc_feature 1696..1746 /label="SynIVS.L" promoter 2874..2902 /label="Amp_promoter" CDS 2944..3804 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2944..3804 /label="Ampicillin" /gene="Ampicillin" rep_origin 3959..4578 /label="pBR322_origin" misc_feature 4794..4820 /label="lacI.C" promoter 4887..4916 /label="lac_promoter" misc_feature 4951..4969 /label="M13r_primer" misc_feature 4983..5033 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG CGTAAGGTAC CGGCAGAAAA 601 AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA AAAATGACTG CTCCAAAGAA 661 GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT 721 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA 781 GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA 841 ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa 901 ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC 961 AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA 1021 AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg 1081 tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC 1141 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG 1201 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA 1261 GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa 1321 ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 1381 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 1441 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 1501 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC 1561 GTAGAATTgt aagtttaaac tttctcatac taactaacgc gctctattta aattttcagA 1621 ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG 1681 GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat 1741 tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA 1801 CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT 1861 AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA 1921 AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT 1981 AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC 2041 TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT 2101 ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC 2161 AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA 2221 GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA 2281 TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA 2341 GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA 2401 AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC 2461 AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG 2521 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 2581 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 2641 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 2701 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 2761 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 2821 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 2881 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 2941 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 3001 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 3061 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 3121 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 3181 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 3241 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 3301 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 3361 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 3421 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 3481 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 3541 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 3601 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 3661 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 3721 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 3781 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 3841 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 3901 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 3961 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 4021 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 4081 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 4141 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 4201 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 4261 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 4321 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 4381 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 4441 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 4501 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 4561 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 4621 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 4681 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 4741 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 4801 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 4861 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 4921 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 4981 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 5041 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1632 LOCUS ADDGENE_1632 4713 bp DNA circular SYN 01/03/2005 DEFINITION L4054 complete plasmid sequence KEYWORDS . SOURCE L4054 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1632 at www.addgene.org FEATURES Location/Qualifiers source 1..4713 /organism="Addgene plasmid L4054" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" CDS 137..1174 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 137..163 /label="SV40.NLS" misc_feature 179..205 /label="SV40.NLS" misc_feature 221..247 /label="SV40.NLS" misc_feature 263..289 /label="SV40.NLS" gene 305..1165 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 476..526 /label="SynIVS.R" misc_feature 548..567 /label="gfp.S65C" misc_feature 681..731 /label="SynIVS.S" misc_feature 896..946 /label="SynIVS.T" misc_feature 1188..1238 /label="SynIVS.M" misc_feature 1315..1365 /label="SynIVS.L" promoter 2493..2521 /label="Amp_promoter" CDS 2563..3423 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2563..3423 /label="Ampicillin" /gene="Ampicillin" rep_origin 3578..4197 /label="pBR322_origin" misc_feature 4413..4439 /label="lacI.C" promoter 4506..4535 /label="lac_promoter" misc_feature 4570..4588 /label="M13r_primer" misc_feature 4602..4652 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG CAGAAAAAAT 181 GACTGCTCCA AAGAAGAAGC GTAAGGTACC GGCAGAAAAA ATGACTGCTC CAAAGAAGAA 241 GCGTAAGGTA CCGGCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGTAGA 301 AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA 361 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA 421 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGgtaag 481 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA CACTTGTCAC 541 TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA GATCATATGA AACgGCATGA 601 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 661 TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac catacatatt 721 taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT 781 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA 841 TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTgtaag 901 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT TCAAAATTAG 961 ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT 1021 TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC 1081 GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG 1141 GATTACACAT GGCATGGATG AACTATACAA ATAGCATTCG TAGAATTgta agtttaaact 1201 ttctcatact aactaacgcg ctctatttaa attttcagAA TTCCAACTGA GCGCCGGTCG 1261 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt 1321 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG 1381 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 1441 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 1501 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 1561 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 1621 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT 1681 CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA 1741 ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA 1801 AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT 1861 GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT 1921 TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT 1981 TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG 2041 GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA 2101 GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 2161 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 2221 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 2281 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 2341 GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT 2401 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 2461 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 2521 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 2581 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 2641 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 2701 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 2761 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 2821 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 2881 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 2941 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 3001 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 3061 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 3121 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 3181 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 3241 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 3301 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 3361 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 3421 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 3481 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 3541 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 3601 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 3661 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 3721 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 3781 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 3841 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 3901 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 3961 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 4021 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 4081 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 4141 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 4201 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 4261 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC 4321 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA 4381 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC 4441 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC 4501 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 4561 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct 4621 tactaactaa ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA 4681 ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1633 LOCUS ADDGENE_1633 6708 bp DNA circular SYN 01/03/2005 DEFINITION L4057 complete plasmid sequence KEYWORDS . SOURCE L4057 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1633 at www.addgene.org FEATURES Location/Qualifiers source 1..6708 /organism="Addgene plasmid L4057" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2503..3672 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTPDNATVWVRKHKDCP PPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQALEFASRPYK" misc_feature 2503..2533 /label="pat3.MbLS" gene 2764..3612 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2935..2985 /label="SynIVS.R" misc_feature 3007..3026 /label="gfp.S65C" misc_feature 3140..3190 /label="SynIVS.S" misc_feature 3355..3405 /label="SynIVS.T" misc_feature 3778..3807 /label="let858.3'" promoter 4488..4516 /label="Amp_promoter" gene 4558..5418 /label="Ampicillin" /gene="Ampicillin" CDS 4558..5418 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5573..6192 /label="pBR322_origin" misc_feature 6408..6434 /label="lacI.C" promoter 6501..6530 /label="lac_promoter" misc_feature 6565..6583 /label="M13r_primer" misc_feature 6597..6647 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGg gtaccgccgg caatgccacc ttcaacatca 2521 ttgctgctcc tcgcagcact tcttccattc gctttaccag caagcgattg gaagactgga 2581 gaagtcactc cggataatgc aacagtctgg gtcagaaaac ataaagattg tcctccacct 2641 gtccctgtgc tcgcaattgt gctcggagtc attgcgggta tcgtaatcct cggaattctt 2701 ctcttgttgc tctggaaatt gctcacagta cttcatgata gatccgaggt accGGTAGAA 2761 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 2821 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 2881 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 2941 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 3001 ACTTTCTgTT ATGGTGTTCA ATGCTTcTCG AGATACCCAG ATCATATGAA ACAGCATGAC 3061 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 3121 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 3181 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 3241 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 3301 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 3361 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 3421 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 3481 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 3541 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 3601 ATTACACATG GCATGGAcGA ACTATACAAA agcccacaAG CTTTGGAATT CGCTAGCCGG 3661 CCATACAAGT AATCCGGATG ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT 3721 GAATATTTGT TTTTTTTCCT ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC 3781 TAAAAAATTA ATTCAATCCA AATCTAAaca tttttttttc tctttccgtc tcccaattcg 3841 tattccgctc ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt 3901 cattaggacg tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga 3961 aataaggaca cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag 4021 aaaacataaa aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag 4081 gcgaacgatg AGAATTGTCC TCGCTTGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG 4141 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 4201 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC 4261 TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC 4321 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC 4381 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 4441 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 4501 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 4561 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 4621 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 4681 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 4741 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 4801 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 4861 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 4921 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 4981 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 5041 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 5101 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 5161 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 5221 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 5281 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 5341 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 5401 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 5461 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 5521 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 5581 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 5641 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 5701 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 5761 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 5821 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 5881 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 5941 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT 6001 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 6061 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 6121 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 6181 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 6241 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 6301 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 6361 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 6421 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC 6481 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT 6541 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa 6601 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG 6661 CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1634 LOCUS ADDGENE_1634 4745 bp DNA circular SYN 01/03/2005 DEFINITION L4058 complete plasmid sequence KEYWORDS . SOURCE L4058 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1634 at www.addgene.org FEATURES Location/Qualifiers source 1..4745 /organism="Addgene plasmid L4058" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 131..161 /label="pat3.MbLS" CDS 131..1261 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTPDNATVWVRKHKDCP PPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" gene 392..1252 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 563..613 /label="SynIVS.R" misc_feature 635..654 /label="gfp.S65C" misc_feature 768..818 /label="SynIVS.S" misc_feature 983..1033 /label="SynIVS.T" misc_feature 1347..1397 /label="SynIVS.L" promoter 2525..2553 /label="Amp_promoter" CDS 2595..3455 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2595..3455 /label="Ampicillin" /gene="Ampicillin" rep_origin 3610..4229 /label="pBR322_origin" misc_feature 4445..4471 /label="lacI.C" promoter 4538..4567 /label="lac_promoter" misc_feature 4602..4620 /label="M13r_primer" misc_feature 4634..4684 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGggt 121 accgccggca atgccacctt caacatcatt gctgctcctc gcagcacttc ttccattcgc 181 tttaccagca agcgattgga agactggaga agtcactccg gataatgcaa cagtctgggt 241 cagaaaacat aaagattgtc ctccacctgt ccctgtgctc gcaattgtgc tcggagtcat 301 tgcgggtatc gtaatcctcg gaattcttct cttgttgctc tggaaattgc tcacagtact 361 tcatgataga tccgaggtac cGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG 421 AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG 481 TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC 541 TGGAAAACTA CCTGTTCCAT GGgtaagttt aaacatatat atactaacta accctgatta 601 tttaaatttt cagCCAACAC TTGTCACTAC TTTCTgTTAT GGTGTTCAAT GCTTcTCgAG 661 ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT 721 ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca 781 gttcggtact aactaaccat acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG 841 TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT 901 TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA 961 ACAAAAGAAT GGAATCAAAG TTgtaagttt aaacatgatt ttactaacta actaatctga 1021 tttaaatttt cagAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC 1081 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA 1141 TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT 1201 CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAATA 1261 GCATTCGTAG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA 1321 AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt 1381 aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT 1441 CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA 1501 AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT 1561 GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC 1621 CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG 1681 TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT 1741 TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT 1801 CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA 1861 ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT 1921 GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC 1981 GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT 2041 TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT 2101 CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC 2161 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 2221 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 2281 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 2341 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA 2401 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA 2461 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 2521 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 2581 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 2641 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 2701 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 2761 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG 2821 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 2881 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 2941 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 3001 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 3061 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 3121 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 3181 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 3241 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 3301 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 3361 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 3421 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 3481 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 3541 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 3601 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 3661 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 3721 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 3781 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 3841 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 3901 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 3961 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT 4021 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 4081 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 4141 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 4201 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 4261 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 4321 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 4381 GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC 4441 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA 4501 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 4561 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG 4621 ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc atttaaattt 4681 tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA ACTTGGAAAT 4741 GAAAT // 1635 LOCUS ADDGENE_1635 6898 bp DNA circular SYN 01/03/2005 DEFINITION L4061 complete plasmid sequence KEYWORDS . SOURCE L4061 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1635 at www.addgene.org FEATURES Location/Qualifiers source 1..6898 /organism="Addgene plasmid L4061" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2736..2760 /label="gfp.Y66H" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3020..3044 /label="gfp.N146I" misc_feature 3039..3062 /label="gfp.M153T" misc_feature 3059..3080 /label="gfp.V163A" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3373..3423 /label="SynIVS.M" misc_feature 3500..3550 /label="SynIVS.L" promoter 4678..4706 /label="Amp_promoter" gene 4748..5608 /label="Ampicillin" /gene="Ampicillin" CDS 4748..5608 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5763..6382 /label="pBR322_origin" misc_feature 6598..6624 /label="lacI.C" promoter 6691..6720 /label="lac_promoter" misc_feature 6755..6773 /label="M13r_primer" misc_feature 6787..6837 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTT TCTGTCACGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTATATTT CACACAACGT ATACATCACT GCCGACAAAC 3061 AAAAGAATGG GATCAAAGCG gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt 3421 cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta ctaactaacg agtaatattt 3541 aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG TCCAATTACT CTTCAACATC 3601 CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC AAAAAACTTC 3661 TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA ATTGTGTAGA 3721 TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT 3781 TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT ATGTTTTTTA 3841 CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA 3901 TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA 3961 CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT 4021 GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC 4081 AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA 4141 GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT 4201 TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA 4261 TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 4321 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 4381 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 4441 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 4501 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 4561 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 4621 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4681 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 4741 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 4801 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 4861 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 4921 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4981 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 5041 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 5101 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 5161 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 5221 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 5281 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 5341 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 5401 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 5461 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 5521 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 5581 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 5641 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 5701 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5761 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 5821 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5881 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 5941 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 6001 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 6061 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 6121 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 6181 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 6241 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 6301 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 6361 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 6421 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 6481 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 6541 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 6601 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 6661 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 6721 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 6781 CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC 6841 TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1636 LOCUS ADDGENE_1636 6843 bp DNA circular SYN 01/03/2005 DEFINITION L4062 complete plasmid sequence KEYWORDS . SOURCE L4062 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1636 at www.addgene.org FEATURES Location/Qualifiers source 1..6843 /organism="Addgene plasmid L4062" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" gene 2490..3350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2490..3359 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2736..2760 /label="gfp.Y66H" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3019..3044 /label="gfp.Y145F" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3445..3495 /label="SynIVS.L" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" misc_feature 6732..6782 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTT TCTGTCACGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTTCAACT CACACAACGT ATACATCATG GCAGACAAAC 3061 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA 3421 ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa 3481 tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 6721 TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc 6781 agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA 6841 AAT // 1637 LOCUS ADDGENE_1637 6898 bp DNA circular SYN 01/03/2005 DEFINITION L4063 complete plasmid sequence KEYWORDS . SOURCE L4063 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1637 at www.addgene.org FEATURES Location/Qualifiers source 1..6898 /organism="Addgene plasmid L4063" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3020..3044 /label="gfp.N146I" misc_feature 3039..3062 /label="gfp.M153T" misc_feature 3059..3080 /label="gfp.V163A" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3373..3423 /label="SynIVS.M" misc_feature 3500..3550 /label="SynIVS.L" promoter 4678..4706 /label="Amp_promoter" gene 4748..5608 /label="Ampicillin" /gene="Ampicillin" CDS 4748..5608 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5763..6382 /label="pBR322_origin" misc_feature 6598..6624 /label="lacI.C" promoter 6691..6720 /label="lac_promoter" misc_feature 6755..6773 /label="M13r_primer" misc_feature 6787..6837 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTT TCTGCTGGGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTATATTT CACACAACGT ATACATCACT GCCGACAAAC 3061 AAAAGAATGG GATCAAAGCG gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt 3421 cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta ctaactaacg agtaatattt 3541 aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG TCCAATTACT CTTCAACATC 3601 CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC AAAAAACTTC 3661 TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA ATTGTGTAGA 3721 TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT 3781 TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT ATGTTTTTTA 3841 CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA 3901 TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA 3961 CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT 4021 GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC 4081 AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA 4141 GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT 4201 TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA 4261 TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 4321 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 4381 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 4441 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 4501 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 4561 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 4621 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4681 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 4741 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 4801 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 4861 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 4921 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4981 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 5041 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 5101 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 5161 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 5221 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 5281 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 5341 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 5401 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 5461 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 5521 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 5581 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 5641 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 5701 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5761 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 5821 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5881 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 5941 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 6001 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 6061 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 6121 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 6181 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 6241 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 6301 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 6361 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 6421 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 6481 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 6541 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 6601 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 6661 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 6721 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 6781 CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC 6841 TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1638 LOCUS ADDGENE_1638 6843 bp DNA circular SYN 01/03/2005 DEFINITION L4064 complete plasmid sequence KEYWORDS . SOURCE L4064 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1638 at www.addgene.org FEATURES Location/Qualifiers source 1..6843 /organism="Addgene plasmid L4064" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2490..3350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3019..3044 /label="gfp.Y145F" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3445..3495 /label="SynIVS.L" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" misc_feature 6732..6782 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTT TCTGCTGGGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTTCAACT CACACAACGT ATACATCATG GCAGACAAAC 3061 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA 3421 ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa 3481 tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 6721 TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc 6781 agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA 6841 AAT // 1639 LOCUS ADDGENE_1639 6898 bp DNA circular SYN 01/03/2005 DEFINITION L4065 complete plasmid sequence KEYWORDS . SOURCE L4065 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1639 at www.addgene.org FEATURES Location/Qualifiers source 1..6898 /organism="Addgene plasmid L4065" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2712..2731 /label="gfp.F64L" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3020..3044 /label="gfp.N146I" misc_feature 3039..3062 /label="gfp.M153T" misc_feature 3059..3080 /label="gfp.V163A" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3373..3423 /label="SynIVS.M" misc_feature 3500..3550 /label="SynIVS.L" promoter 4678..4706 /label="Amp_promoter" CDS 4748..5608 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4748..5608 /label="Ampicillin" /gene="Ampicillin" rep_origin 5763..6382 /label="pBR322_origin" misc_feature 6598..6624 /label="lacI.C" promoter 6691..6720 /label="lac_promoter" misc_feature 6755..6773 /label="M13r_primer" misc_feature 6787..6837 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTC TCACTTGGGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTATATTT CACACAACGT ATACATCACT GCCGACAAAC 3061 AAAAGAATGG GATCAAAGCG gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt 3421 cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta ctaactaacg agtaatattt 3541 aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG TCCAATTACT CTTCAACATC 3601 CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC AAAAAACTTC 3661 TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA ATTGTGTAGA 3721 TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT 3781 TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT ATGTTTTTTA 3841 CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA 3901 TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA 3961 CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT 4021 GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC 4081 AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA 4141 GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT 4201 TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA 4261 TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 4321 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 4381 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 4441 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 4501 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 4561 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 4621 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4681 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 4741 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 4801 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 4861 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 4921 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4981 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 5041 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 5101 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 5161 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 5221 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 5281 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 5341 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 5401 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 5461 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 5521 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 5581 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 5641 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 5701 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5761 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 5821 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5881 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 5941 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 6001 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 6061 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 6121 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 6181 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 6241 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 6301 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 6361 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 6421 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 6481 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 6541 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 6601 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 6661 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 6721 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 6781 CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC 6841 TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1640 LOCUS ADDGENE_1640 6843 bp DNA circular SYN 01/03/2005 DEFINITION L4066 complete plasmid sequence KEYWORDS . SOURCE L4066 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1640 at www.addgene.org FEATURES Location/Qualifiers source 1..6843 /organism="Addgene plasmid L4066" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2490..3350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2712..2731 /label="gfp.F64L" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3019..3044 /label="gfp.Y145F" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3445..3495 /label="SynIVS.L" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" misc_feature 6732..6782 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTC TCACTTGGGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTTCAACT CACACAACGT ATACATCATG GCAGACAAAC 3061 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA 3421 ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa 3481 tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 6721 TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc 6781 agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA 6841 AAT // 1641 LOCUS ADDGENE_1641 6898 bp DNA circular SYN 01/03/2005 DEFINITION L4095 complete plasmid sequence KEYWORDS . SOURCE L4095 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1641 at www.addgene.org FEATURES Location/Qualifiers source 1..6898 /organism="Addgene plasmid L4095" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2712..2731 /label="gfp.F64L" misc_feature 2736..2760 /label="gfp.Y66H" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3020..3044 /label="gfp.N146I" misc_feature 3039..3062 /label="gfp.M153T" misc_feature 3059..3080 /label="gfp.V163A" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3373..3423 /label="SynIVS.M" misc_feature 3500..3550 /label="SynIVS.L" promoter 4678..4706 /label="Amp_promoter" gene 4748..5608 /label="Ampicillin" /gene="Ampicillin" CDS 4748..5608 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5763..6382 /label="pBR322_origin" misc_feature 6598..6624 /label="lacI.C" promoter 6691..6720 /label="lac_promoter" misc_feature 6755..6773 /label="M13r_primer" misc_feature 6787..6837 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTC TCACTCACGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTATATTT CACACAACGT ATACATCACT GCCGACAAAC 3061 AAAAGAATGG GATCAAAGCG gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt 3421 cagAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta ctaactaacg agtaatattt 3541 aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG TCCAATTACT CTTCAACATC 3601 CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC AAAAAACTTC 3661 TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA ATTGTGTAGA 3721 TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT 3781 TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT ATGTTTTTTA 3841 CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA 3901 TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA 3961 CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT 4021 GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC 4081 AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA 4141 GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT 4201 TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA 4261 TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 4321 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 4381 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 4441 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 4501 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 4561 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 4621 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4681 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 4741 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 4801 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 4861 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 4921 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4981 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 5041 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 5101 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 5161 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 5221 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 5281 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 5341 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 5401 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 5461 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 5521 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 5581 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 5641 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 5701 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5761 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 5821 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5881 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 5941 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 6001 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 6061 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 6121 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 6181 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 6241 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 6301 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 6361 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 6421 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 6481 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 6541 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 6601 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 6661 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 6721 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 6781 CAAGCTgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagAGC 6841 TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA ACAACTTGGA AATGAAAT // 1642 LOCUS ADDGENE_1642 6843 bp DNA circular SYN 01/03/2005 DEFINITION L4096 complete plasmid sequence KEYWORDS . SOURCE L4096 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1642 at www.addgene.org FEATURES Location/Qualifiers source 1..6843 /organism="Addgene plasmid L4096" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2455..2473) /label="T7_promoter" CDS 2490..3359 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTHGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 2490..3350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2661..2711 /label="SynIVS.R" misc_feature 2712..2731 /label="gfp.F64L" misc_feature 2736..2760 /label="gfp.Y66H" misc_feature 2866..2916 /label="SynIVS.S" misc_feature 3019..3044 /label="gfp.Y145F" misc_feature 3081..3131 /label="SynIVS.T" misc_feature 3445..3495 /label="SynIVS.L" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" misc_feature 6732..6782 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GACCGCGGTT TTGGCGCGGA TCCTATCGAT TCGCGGCCGC TGTACACCCG GGTGCCCTAT 2461 AGTGAGTCGT ATTGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG 2521 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 2581 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 2641 GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt 2701 taaattttca gCCAACACTT GTCACTACTC TCACTCACGG CGTACAATGC TTCTCGAGAT 2761 ACCCAGATCA TATGAAACAG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 2821 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt 2881 tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG 2941 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC 3001 TTGGACACAA ATTGGAATAC AACTTCAACT CACACAACGT ATACATCATG GCAGACAAAC 3061 AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt 3121 taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 3181 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 3241 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 3301 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC 3361 ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA 3421 ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa 3481 tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 6721 TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc 6781 agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA 6841 AAT // 1643 LOCUS ADDGENE_1643 3611 bp DNA circular SYN 01/03/2005 DEFINITION L4119 complete plasmid sequence KEYWORDS . SOURCE L4119 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1643 at www.addgene.org FEATURES Location/Qualifiers source 1..3611 /organism="Addgene plasmid L4119" /mol_type="other DNA" promoter complement(766..784) /label="T7_promoter" misc_feature 804..952 /label="lacZ_a" misc_feature complement(795..811) /label="M13f20_primer" rep_origin 970..1276 /label="f1_origin" promoter 1469..1497 /label="Amp_promoter" gene 1539..2399 /label="Ampicillin" /gene="Ampicillin" CDS 1539..2399 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2554..3173 /label="pBR322_origin" misc_feature 3389..3415 /label="lacI.C" promoter 3482..3511 /label="lac_promoter" misc_feature 3546..3564 /label="M13r_primer" promoter 3581..3600 /label="T3_promoter" ORIGIN 1 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTggat ccaCCGGTGC TCCACTATTA 61 GAGGATACGG AATGCTGTGG AAAAGAGAGA TGTCAGCATG CGACTGAAGA ATCTGAATAC 121 AAGAAACTTG TATATGTAGC TGTGCATCTT GTGAATGTAC TCGAACGACT ATCTCTTCCT 181 TCTGCACATA TCGATGATTC CGATGAGGAA AAAGAGCCGA AAGCCGACGT TCGAAGACTG 241 ATGATCGTGT GCCATGAATT ACATATTCCA CTTCTACATC ATACACTTGA AGAGAGGATT 301 CCTGATAGTA ACAGTGCAGA GTTGGGACTT CCCAAAGCTG CAGTTTTGGA ACAACTTCTA 361 GAACTTGACC TAGATGTGAA TGCCACTGAT AAGAATAACG ACACTCCAAT GCACATTCTG 421 CTTCGAGCCA GAGAGTTTAG GAAAAGCTTG GTTCGAGCAC TGCTCGTACG TGGCACATGG 481 CTGTTCGCAA GGAATCGGCA CGGAGATGTG GTGTTGAATG TGATGAAACG TATGAAAGCG 541 TTGAATCACG CCAACTTTGA TGATCTACCA CTAGGTGCGT GTCGAATCAG AAATTGAGAG 601 TTATTAGATT ATTTGGAACG CGAATAACTT CATTATACCA CGGAGAGAGC CAGAAATTGA 661 AGAAATTGGT TAAGGAGAAT GGAATTTAAT GAATAATAGC GCACCAATTC GATATCAAGC 721 TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA ATTCGCCCTA TAGTGAGTCG 781 TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 841 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 901 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC 961 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 1021 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 1081 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 1141 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 1201 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 1261 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 1321 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 1381 TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 1441 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 1501 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 1561 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 1621 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 1681 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 1741 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 1801 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 1861 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 1921 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 1981 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 2041 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 2101 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 2161 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 2221 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 2281 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 2341 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 2401 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 2461 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 2521 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 2581 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 2641 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 2701 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 2761 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 2821 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 2881 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 2941 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 3001 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 3061 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 3121 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 3181 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 3241 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 3301 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 3361 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 3421 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 3481 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 3541 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG 3601 AACAAAAGCT G // 1644 LOCUS ADDGENE_1644 3642 bp DNA circular SYN 01/03/2005 DEFINITION L4129 complete plasmid sequence KEYWORDS . SOURCE L4129 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1644 at www.addgene.org FEATURES Location/Qualifiers source 1..3642 /organism="Addgene plasmid L4129" /mol_type="other DNA" CDS 52..804 /label="Orf0" /translation="MASHVTRGSPGLQEFDIKLIDTVDLEDAKRRQAQKINELQEALD AANSKNASLEKTKSRLVGDLDDAQVDVERANGVASALEKKQKGFDKIIDEWRKKTDDL AAELDGAQRDLRNTSTDLFKAKNAQEELAEVVEGLRRENKSLSQEIKDLTDQLGEGGR SVHEMQKIIRRLEIEKEELQHALDEAEAALEAEESKVLRAQVEVSQIRSEIEKRIQEK EEEFENTRKNHARALESMQASLEGGPGTQFAL" promoter complement(797..815) /label="T7_promoter" misc_feature 835..983 /label="lacZ_a" misc_feature complement(826..842) /label="M13f20_primer" rep_origin 1001..1307 /label="f1_origin" promoter 1500..1528 /label="Amp_promoter" gene 1570..2430 /label="Ampicillin" /gene="Ampicillin" CDS 1570..2430 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2585..3204 /label="pBR322_origin" misc_feature 3420..3446 /label="lacI.C" promoter 3513..3542 /label="lac_promoter" misc_feature 3577..3595 /label="M13r_primer" promoter 3612..3631 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gaccTCGAGG ATGCCAAGAG ACGCCAAGCC CAAAAGATCA ACGAGCTCCA AGAGGCTCTT 181 GATGCCGCCA ACTCTAAGAA CGCTTCTCTT GAGAAGACCA AGTCCAGACT TGTCGGAGAC 241 TTGGACGACG CTCAAGTTGA TGTCGAGAGA GCCAACGGTG TCGCCAGTGC TCTTGAGAAG 301 AAGCAAAAGG GCTTCGACAA GATCATCGAC GAATGGAGAA AGAAGACCGA CGACTTGGCC 361 GCTGAGCTTG ATGGAGCTCA ACGCGACCTT CGCAACACCT CCACTGATCT CTTCAAGGCC 421 AAGAACGCCC AAGAGGAGCT CGCCGAGGTT GTTGAGGGAC TCCGCCGTGA GAACAAGAGC 481 TTGAGCCAAG AGATCAAGGA TCTTACCGAT CAACTCGGAG AGGGAGGACG CTCTGTCCAC 541 GAAATGCAAA AGATCATCCG CCGTCTTGAG ATTGAGAAGG AAGAACTCCA ACACGCTTTG 601 GACGAGGCTG AGGCTGCCCT TGAAGCTGAA GAGAGCAAGG TTCTCCGCGC CCAGGTTGAA 661 GTTTCCCAGA TCCGTTCCGA AATCGAGAAA CGCATCCAGG AGAAGGAGGA AGAGTTCGAG 721 AACACGAGAA AGAACCACGC CCGCGCTCTT GAATCAATGC AAGCTTCCCt cgaggggggg 781 cccggtaccC AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT 841 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 901 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT 961 GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 1021 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 1081 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 1141 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 1201 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 1261 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 1321 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 1381 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA 1441 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 1501 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 1561 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 1621 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 1681 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 1741 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 1801 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 1861 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 1921 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 1981 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 2041 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 2101 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 2161 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 2221 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 2281 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 2341 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 2401 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 2461 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 2521 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 2581 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 2641 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 2701 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 2761 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 2821 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 2881 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 2941 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 3001 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 3061 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 3121 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 3181 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 3241 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 3301 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 3361 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 3421 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 3481 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 3541 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC 3601 GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TG // 1645 LOCUS ADDGENE_1645 3726 bp DNA circular SYN 01/03/2005 DEFINITION L4138 complete plasmid sequence KEYWORDS . SOURCE L4138 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1645 at www.addgene.org FEATURES Location/Qualifiers source 1..3726 /organism="Addgene plasmid L4138" /mol_type="other DNA" rep_origin complement(133..439) /label="f1_origin" misc_feature 598..614 /label="M13f20_primer" misc_feature complement(457..617) /label="lacZ_a" promoter 622..640 /label="T7_promoter" gene 657..1472 /label="lacZ(2028-2844)" /gene="lacZ(2028-2844)" CDS 663..1478 /label="Orf2" /translation="MVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTV RVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRW QFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAG HYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEV ASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDM" CDS complement(239..946) /label="Orf1" /translation="MQKSISLVVRCGMAWDAAGSVTLRFSARRHCCQALMCPASDHAV AFGCTTRTVSQSCPALSGCGSSGSSINCLPCGATSRGTSPLASGLPSSATIQCRSSNS PYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLP LGFRFSALRHLDPKKLD" promoter complement(1534..1553) /label="T3_promoter" misc_feature complement(1570..1588) /label="M13r_primer" misc_feature complement(1719..1745) /label="lacI.C" rep_origin complement(1961..2580) /label="pBR322_origin" gene complement(2735..3595) /label="Ampicillin" /gene="Ampicillin" CDS complement(2735..3595) /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(3637..3665) /label="Amp_promoter" ORIGIN 1 AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT 61 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA 121 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA 181 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT 241 AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC 301 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 361 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA 421 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTACGCA 481 ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAGGGGG 541 GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA 601 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCCTGCACT 661 GGATGGTGGC GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC 721 CACAAGGTAA ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC 781 TCTGGCTCAC AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA 841 TCAGCGCCTG GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT 901 CCCACGCCAT CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA 961 AGCGTTGGCA ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA 1021 AACAACTGCT GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG 1081 GCGTAAGTGA AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG 1141 GCCATTACCA GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG 1201 TGCTGATTAC GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA 1261 AAACCTACCG GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA 1321 GCGATACACC GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC 1381 GGGTAAACTG GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT 1441 GTTTTGACCG CTGGGATCTG CCATTGTCAG ACATGTAATC AAGCTTATCG ATACCGTCGA 1501 CCTCGAGGGG GGGCCCGGTA CCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTCCGAGCT 1561 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 1621 ACAACATAGG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGGTAAC 1681 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 1741 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 1801 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 1861 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 1921 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 1981 ATAGGCTCGG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 2041 ACCCGACAGG ACTATAAAGA TACCAGGCGT TCCCCCCTGG AAGCTCCCTC GTGCGCTCTC 2101 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 2161 CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 2221 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 2281 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 2341 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 2401 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 2461 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 2521 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 2581 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 2641 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 2701 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 2761 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTGCCC GTCGTGTAGA 2821 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 2881 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 2941 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 3001 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 3061 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 3121 GAGTTACATG ATCCCCCATG TTGTGAAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 3181 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGCT TATGGCAGCA CTGCATAATT 3241 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 3301 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 3361 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 3421 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 3481 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 3541 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 3601 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 3661 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 3721 CACCTG // 1646 LOCUS ADDGENE_1646 5248 bp DNA circular SYN 01/03/2005 DEFINITION L4271 complete plasmid sequence KEYWORDS . SOURCE L4271 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1646 at www.addgene.org FEATURES Location/Qualifiers source 1..5248 /organism="Addgene plasmid L4271" /mol_type="other DNA" misc_feature 13..31 /label="M13r_primer" CDS 23..1825 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf1" /translation="MTMITPSSKLTLTKGNKSWVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHM KRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVL LPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPGDPLVLERPPLE VDGIDKLDIEFGRWVPCAKVKDTKAHIDGLKKGQTYQFRVKAVNKEGASDALSTDKDT KAKNPYDEPGKTGTPDVVDWDADRVSLEWEPPKSDGGAPITQYVIEKKGKHGRDWQEC GKVSGDQTNAEILGLKEGEEYQFRVKAVNKAGPGEASDPSRKVVAKPRNLKPWIDREA MKTITIKVGNDVEFDVPVRGEPPPKKEWIFNEKPVDDQKIRIESEDYKTRFVLRGATR KHAGLYTLTATNASGSDKHSVEVIVLGGDPLVLERPPLEVDGIDKLDIEFYECYLYSS SMPCVIPAAVTNSRRTMWSLFSLGSFERADCVDR" promoter 48..67 /label="T3_promoter" gene 95..802 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 278..301 /label="gfp.S65S" misc_feature complement(2215..2238) /label="gfp.S65S" gene complement(1717..2421) /label="GFP(variant)" /gene="GFP(variant)" CDS complement(1705..2421) /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK" promoter complement(2484..2502) /label="T7_promoter" misc_feature 2507..2667 /label="lacZ_a" misc_feature complement(2510..2526) /label="M13f20_primer" rep_origin 2685..2991 /label="f1_origin" promoter 3184..3212 /label="Amp_promoter" gene 3254..4114 /label="Ampicillin" /gene="Ampicillin" CDS 3254..4114 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4269..4888 /label="pBR322_origin" misc_feature 5104..5130 /label="lacI.C" promoter 5197..5226 /label="lac_promoter" ORIGIN 1 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac 61 taaagggaac aaaagctggg tacCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC 121 TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT 181 CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC 241 TACTGGAAAA CTACCTGTTC CATGGCCAAC ACTTGTCACT ACTTTCTCTT ATGGTGTTCA 301 ATGCTTTTCA AGATACCCAG ATCATATGAA ACgGCATGAC TTTTTCAAGA GTGCCATGCC 361 CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACG 421 TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA 481 TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA 541 TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTAACTTCA AAATTAGACA 601 CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG 661 CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA 721 AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT 781 TACACATGGC ATGGATGAAC TATACAAATg cccgggggat ccactagttc tagagcggcc 841 ccccctcgag gtcgacggta tcgataagct tgatatcgAA TTCGGAAGAT GGGTTCCATG 901 CGCCAAAGTT AAGGATACGA AGGCTCATAT TGATGGTCTC AAGAAGGGAC AAACATATCA 961 GTTCCGTGTC AAGGCTGTCA ATAAGGAAGG AGCTTCTGAT GCATTGTCTA CTGATAAGGA 1021 CACCAAAGCC AAGAATCCAT ATGATGAGCC AGGAAAAACC GGAACTCCGG ATGTTGTCGA 1081 CTGGGATGCT GATCGTGTCT CACTTGAATG GGAACCACCA AAGTCTGACG GAGGAGCTCC 1141 AATCACTCAA TACGTCATTG AGAAGAAGGG CAAACATGGA AGAGACTGGC AAGAATGCGG 1201 AAAGGTTTCT GGAGATCAAA CCAATGCTGA GATTCTTGGA CTCAAGGAGG GAGAAGAGTA 1261 CCAGTTCCGT GTGAAGGCTG TTAACAAGGC CGGACCGGGA GAGGCTTCAG ACCCAAGCCG 1321 AAAGGTTGTT GCAAAGCCAA GAAACTTGAA GCCATGGATT GATCGTGAAG CAATGAAGAC 1381 GATCACTATC AAGGTAGGAA ACGATGTGGA ATTCGATGTT CCAGTACGCG GAGAACCACC 1441 ACCGAAGAAG GAATGGATCT TCAATGAGAA ACCAGTCGAT GATCAAAAGA TCAGGATTGA 1501 AAGCGAAGAC TACAAGACCC GATTTGTGCT CCGTGGAGCA ACTCGCAAGC ATGCTGGTTT 1561 GTACACTCTT ACTGCTACCA ACGCTTCTGG AAGCGACAAA CATTCCGTTG AGGTCATTGT 1621 GCTCGGgggg gatccactag ttctagagcG GCCCCCCCTC GAGGTCGACG GTATCGATAA 1681 GCTTGATATC GAATTCTACG AATGCTATTT GTATAGTTCA TCCATGCCAT GTGTAATCCC 1741 AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC TTTTCGTTGG GATCTTTCGA 1801 AAGGGCAGAT TGTGTGGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT 1861 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCTT CCATCTTCAA TGTTGTGTCT 1921 AATTTTGAAG TTAACTTTGA TTCCATTCTT TTGTTTGTCT GCCATGATGT ATACATTGTG 1981 TGAGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT CCATCTTCTT TAAAATCAAT 2041 ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA AACTTGACTT CAGCACGTGT 2101 CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC TGTACATAAC CTTCGGGCAT 2161 GGCACTCTTG AAAAAGTCAT GCcGTTTCAT ATGATCTGGG TATCTTGAAA AGCATTGAAC 2221 ACCATAAGAG AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA 2281 AATAAATTTA AGGGTAAGTT TTCCGTATGT TGCATCACCT TCACCCTCTC CACTGACAGA 2341 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA 2401 AAGTTCTTCT CCTTTACTCA TTTTTTCTAc cGGGGGATCC ACTAGTTCTA GAGCGGCCGC 2461 CACCGCGGTG GAGCTCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG 2521 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC 2581 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC 2641 AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 2701 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 2761 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGccggctt tccccgtcaa gctctaaatc 2821 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 2881 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 2941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 3001 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 3061 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 3121 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 3181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 3241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 3301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 3361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 3421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 3481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 3541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 3601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 3661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 3721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 3781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 4321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 4381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 4441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 4501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 4681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 4981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 5041 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 5101 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 5161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 5221 tatgttgtgt ggaattgtga gcggataa // 1647 LOCUS ADDGENE_1647 5029 bp DNA circular SYN 01/03/2005 DEFINITION L4400 complete plasmid sequence KEYWORDS . SOURCE L4400 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1647 at www.addgene.org FEATURES Location/Qualifiers source 1..5029 /organism="Addgene plasmid L4400" /mol_type="other DNA" rep_origin complement(133..439) /label="f1_origin" misc_feature 598..614 /label="M13f20_primer" misc_feature complement(457..617) /label="lacZ_a" promoter 622..640 /label="T7_promoter" CDS complement(239..1774) /label="Orf1" /translation="MAWMNYTNSIRRIPAAQLNVFVLDAPGKPTGPIRATDIQADAMT LSWRPPKDNGGDAITNYVVEKRTPGGDWVTVGHPVGTTLRVRNLDANTPYEFRVRAEN QYGVGEPLETDDAIVAKNPFDTPGAPGQPEAVETSEEAITLQWTRPTSDGGAPIQGYV IEKREVGSTEWTKAAFGNILDTKHRVTGLTPKKTYEFRVAAYNAAGQGEYSVNSVPIT ADNAPTRPKINMGMLTRDILAYAGERAKILVPFAASPAPKVTFSKGENKISPTDPRVK VEYSDFLATLTIEKSELTDGGLYFVELENSQGSDSASIRLKVVDKPASPQHIRVEDIA PDCCTLYWMPPSSDGGSPITNYIVEKLGDPLVLERPPPRWSSNSPYSESYYNSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAA GVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPK KLD" CDS 2059..2844 /label="Orf0" /translation="MFPSSLKSIPFNSILLTRVSPSNLTSAPPIQGYVIEKREVGSTE WTKAAFGNILDTKHRVTGLTPKKTYEFRVAAYNAAGQGEYSVNSVPITADNAPTRPKI NMGMLTRDILAYAGERAKILVPFAASPAPKVTFSKGENKISPTDPRVKVEYSDFLATL TIEKSELTDGGLYFVELENSQGSDSASIRLKVVDKPASPQHIRVEDIAPDCCTLYWMP PSSDGGSPITNYIVEKLGCRNSISSLSIPSTSRGGPVPSFCSL" gene complement(1761..2132) /label="GFP(384-12)" /gene="GFP(384-12)" promoter complement(2837..2856) /label="T3_promoter" misc_feature complement(2873..2891) /label="M13r_primer" misc_feature complement(3022..3048) /label="lacI.C" rep_origin complement(3264..3883) /label="pBR322_origin" gene complement(4038..4898) /label="Ampicillin" /gene="Ampicillin" CDS complement(4038..4898) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(4940..4968) /label="Amp_promoter" ORIGIN 1 AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT 61 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA 121 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA 181 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT 241 AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC 301 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 361 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA 421 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTACGCA 481 ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAGGGGG 541 GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA 601 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCCACCGCG 661 GTGGCGGCCG CTCTAGAACT AGTGGATCCC CCAGCTTCTC AACGATGTAG TTTGTGATTG 721 GCGATCCTCC ATCAGATGAT GGTGGCATCC AGTAAAGAGT GCAGCAGTCT GGTGCAATAT 781 CCTCTACTCG GATGTGTTGT GGTGAAGCTG GTTTATCAAC AACCTTCAAT CTGATACTAG 841 CAGAATCACT TCCTTGACTA TTTTCAAGTT CAACAAAGTA GAGTCCACCA TCAGTAAGTT 901 CAGACTTCTC GATCGTCAAT GTAGCCAAGA AGTCACTGTA CTCAACCTTC ACTCTTGGAT 961 CAGTTGGGCT GATCTTATTC TCACCCTTCG AGAAAGTGAC TTTCGGAGCA GGTGAAGCAG 1021 CAAATGGAAC AAGAATCTTT GCACGTTCTC CAGCGTAAGC GAGGATATCG CGAGTAAGCA 1081 TTCCCATGTT GATCTTCGGT CTTGTTGGAG CATTATCAGC TGTAATTGGA ACAGAGTTAA 1141 CACTGTATTC TCCTTGTCCA GCTGCATTGT AAGCTGCAAC TCTGAATTCG TAGGTCTTCT 1201 TTGGAGTGAG TCCAGTGACA CGATGTTTTG TGTCAAGGAT GTTTCCAAAT GCAGCTTTCG 1261 TCCATTCAGT TGATCCGACT TCTCTCTTCT CAATCACATA TCCTTGAATT GGAGCTCCTC 1321 CATCAGATGT TGGTCTTGTC CATTGAAGTG TAATTGCTTC CTCCGAAGTT TCAACGGCTT 1381 CTGGTTGTCC TGGAGCTCCT GGAGTATCGA ACGGATTCTT GGCAACAATT GCGTCATCAG 1441 TTTCAAGTGG CTCTCCAACT CCATATTGAT TTTCAGCGCG AACACGGAAT TCGTAAGGAG 1501 TATTGGCATC CAAATTGCGG ACACGAAGAG TTGTTCCAAC AGGATGTCCG ACAGTGACCC 1561 AGTCACCGCC TGGAGTTCTC TTTTCAACGA CATAATTGGT GATAGCATCT CCTCCATTAT 1621 CCTTTGGTGG TCTCCATGAA AGCGTCATTG CATCGGCTTG AATGTCAGTA GCACGGATTG 1681 GTCCAGTTGG TTTTCCTGGA GCATCAAGAA CGAAGACATT AAGCTGGGCT GCAGGAATTC 1741 TACGAATGCT ATTTGTATAG TTCATCCATG CCATGTGTAA TCCCAGCAGC TGTTACAAAC 1801 TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT TCGAAAGGGC AGATTGTGTG 1861 GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC CAATTGGAGT ATTTTGTTGA 1921 TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT GTCTAATTTT GAAGTTAACT 1981 TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT TGTGTGAGTT ATAGTTGTAT 2041 TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT CAATACCTTT TAACTCGATT 2101 CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC CTCCAATTCA AGGATATGTG 2161 ATTGAGAAGA GAGAAGTCGG ATCAACTGAA TGGACGAAAG CTGCATTTGG AAACATCCTT 2221 GACACAAAAC ATCGTGTCAC TGGACTCACT CCAAAGAAGA CCTACGAATT CAGAGTTGCA 2281 GCTTACAATG CAGCTGGACA AGGAGAATAC AGTGTTAACT CTGTTCCAAT TACAGCTGAT 2341 AATGCTCCAA CAAGACCGAA GATCAACATG GGAATGCTTA CTCGCGATAT CCTCGCTTAC 2401 GCTGGAGAAC GTGCAAAGAT TCTTGTTCCA TTTGCTGCTT CACCTGCTCC GAAAGTCACT 2461 TTCTCGAAGG GTGAGAATAA GATCAGCCCA ACTGATCCAA GAGTGAAGGT TGAGTACAGT 2521 GACTTCTTGG CTACATTGAC GATCGAGAAG TCTGAACTTA CTGATGGTGG ACTCTACTTT 2581 GTTGAACTTG AAAATAGTCA AGGAAGTGAT TCTGCTAGTA TCAGATTGAA GGTTGTTGAT 2641 AAACCAGCTT CACCACAACA CATCCGAGTA GAGGATATTG CACCAGACTG CTGCACTCTT 2701 TACTGGATGC CACCATCATC TGATGGAGGA TCGCCAATCA CAAACTACAT CGTTGAGAAG 2761 CTGGGCTGCA GGAATTCGAT ATCAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCG 2821 GTACCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTCCGA GCTTGGCGTA ATCATGGTCA 2881 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT AGGAGCCGGA 2941 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGGT AACTCACATT AATTGCGTTG 3001 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 3061 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC 3121 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 3181 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 3241 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CGGCCCCCCT 3301 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 3361 AGATACCAGG CGTTCCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 3421 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA 3481 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 3541 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 3601 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 3661 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG 3721 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 3781 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 3841 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 3901 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 3961 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG 4021 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT 4081 CTATTTCGTT CATCCATAGT TGCCTGACTG CCCGTCGTGT AGATAACTAC GATACGGGAG 4141 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA 4201 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT 4261 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA 4321 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG 4381 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC 4441 ATGTTGTGAA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG 4501 GCCGCAGTGT TATCACTCAT GCTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA 4561 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT 4621 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC 4681 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC 4741 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA 4801 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA 4861 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT 4921 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 4981 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTG // 1648 LOCUS ADDGENE_1648 4669 bp DNA circular SYN 01/03/2005 DEFINITION L4404 complete plasmid sequence KEYWORDS . SOURCE L4404 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1648 at www.addgene.org FEATURES Location/Qualifiers source 1..4669 /organism="Addgene plasmid L4404" /mol_type="other DNA" promoter complement(1824..1842) /label="T7_promoter" misc_feature 1862..2010 /label="lacZ_a" misc_feature complement(1853..1869) /label="M13f20_primer" rep_origin 2028..2334 /label="f1_origin" promoter 2527..2555 /label="Amp_promoter" CDS 2597..3457 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2597..3457 /label="Ampicillin" /gene="Ampicillin" rep_origin 3612..4231 /label="pBR322_origin" misc_feature 4447..4473 /label="lacI.C" promoter 4540..4569 /label="lac_promoter" misc_feature 4604..4622 /label="M13r_primer" promoter 4639..4658 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg cccccccTCG AGTTGAGAAG TAAGCTGGGA CTTCAGTCTG 61 GTGAGTTGGT TGACTTGGGA TTCGGCATCT TCGAGTTGAC GGACAAGGTC TCCATTCTCA 121 GAGTGAAGGC GTCCCTTAAG GGAAGTGAAG TCTTGGAGTT GGCGAGATTG CTCATCAGCC 181 TTAGATTGAA GCTCGGTGAG TTGAAGTTCG AACTGCTTGG CAAGCTTTTC GTTGTTGAGC 241 TTTCCAGAGG TTTCTTGGTC AAGTTGAGCA GCAAGGTCCT CAGCGTCACG AACAGCTTGA 301 GCCTTGTCCT TCTCGACCTT AGCCTTGGCC TTGTTGAGTT GATCGAGTTG GTCGGTGAGC 361 TCAGCGACAG CGTCGGTGTG CTTCTTGCGA AGTCCACCGA GTTGGTTCTC GTGGTTCATG 421 TTGGCCTCCT CCAAGTCTCT GCGGAGCTTG GCAAGTTCAG CCTCACGCTT CTTGTTGACC 481 TCAACCTGGG CGGCAGTAGC TCCACCTTGC TCGTCAAGCT TCTCACCGAG CTCTTCCAAC 541 TCGCGTTGGA GGTCGCTCTT GGCACGGTCA GCCTTGGAAC GGGATTGACG TTCATTCTCG 601 Aggtcgacgg tatcgataag cttgatatcg aattcctgca gtatttaaaa atcgaaacta 661 gaatgtttcc tatttcagAC CTACTCCGGA CTTTTCTGCG TTGTCATCAA CCCATACAAG 721 CGTCTCCCAA TCTACACCGA CTCTTGCGCC CGTATGTTCA TGGGAAAGAG AAAGACAGAA 781 ATGCCACCAC ATTTGTTCGC TGTCTCTGAC GAAGCCTACC GTAACATGCT CCAAGACCAC 841 GAGAACCAGT CTATGCTCAT TACCGGAGAA TCTGGAGCCG GAAAGACTGA GAACACCAAG 901 AAGGTTATTT GCTACTTCGC CGCTGTCGGT GCCTCCCAAC AGGAAGGTGG AGCTGAAGTT 961 GATCCAAATA AGAAGAAGGT CACCCTTGAG GACCAAATCG TCCAGACCAA TCCAGTACTT 1021 GAAGCCTTCG GTAACGCCAA GACTGTCCGT AACAACAACT CATCCCGTTT CGGAAAGTTC 1081 ATCCGTATCC ACTTCAACAA GCACGGACGC CTCGCATCTT GCGATATCGA GCATTgtgag 1141 ttactttttg atttatattc tagcatagct caataaaaat tatgcaacta ttgtttaaac 1201 tataaatttt gcagACTTGC tcgaccTCGA GAATGAACGT CAATCCCGTT CCAAGGCTGA 1261 CCGTGCCAAG AGCGACCTCC AACGCGAGTT GGAAGAGCTC GGTGAGAAGC TTGACGAGCA 1321 AGGTGGAGCT ACTGCCGCCC AGGTTGAGGT CAACAAGAAG CGTGAGGCTG AACTTGCCAA 1381 GCTCCGCAGA GACTTGGAGG AGGCCAACAT GAACCACGAG AACCAACTCG GTGGACTTCG 1441 CAAGAAGCAC ACCGACGCTG TCGCTGAGCT CACCGACCAA CTCGATCAAC TCAACAAGGC 1501 CAAGGCTAAG GTCGAGAAGG ACAAGGCTCA AGCTGTTCGT GACGCTGAGG ACCTTGCTGC 1561 TCAACTTGAC CAAGAAACCT CTGGAAAGCT CAACAACGAA AAGCTTGCCA AGCAGTTCGA 1621 ACTTCAACTC ACCGAGCTTC AATCTAAGGC TGATGAGCAA TCTCGCCAAC TCCAAGACTT 1681 CACTTCCCTT AAGGGACGCC TTCACTCTGA GAATGGAGAC CTTGTCCGTC AACTCGAAGA 1741 TGCCGAATCC CAAGTCAACC AACTCACCAG ACTGAAGTCC CAGCTTACTT CTCAACTCGA 1801 gggggggccc ggtaccCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG 1861 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 1921 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC 1981 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 2041 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 2101 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 2161 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 2221 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 2281 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 2341 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 2401 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA 2461 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 2521 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 2581 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 2641 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 2701 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 2761 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 2821 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 2881 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 2941 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 3001 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3061 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 3121 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3181 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 3241 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 3301 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 3361 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 3421 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 3481 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 3541 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 3601 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3661 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3721 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 3781 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3841 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3901 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3961 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4021 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4081 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4141 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4201 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4261 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 4321 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 4381 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 4441 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 4501 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 4561 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA 4621 TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTG // 1649 LOCUS ADDGENE_1649 3489 bp DNA circular SYN 01/03/2005 DEFINITION L4417 complete plasmid sequence KEYWORDS . SOURCE L4417 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1649 at www.addgene.org FEATURES Location/Qualifiers source 1..3489 /organism="Addgene plasmid L4417" /mol_type="other DNA" promoter 23..41 /label="T7_promoter" misc_feature complement(631..654) /label="gfp.S65S" gene complement(133..837) /label="GFP(variant)" /gene="GFP(variant)" CDS complement(34..837) /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYKCPGDPLVLERPPPRWSSNSSMISDLPVSL" promoter complement(861..879) /label="T7_promoter" misc_feature 899..1047 /label="lacZ_a" misc_feature complement(890..906) /label="M13f20_primer" rep_origin 1065..1371 /label="f1_origin" promoter 1564..1592 /label="Amp_promoter" gene 1634..2494 /label="Ampicillin" /gene="Ampicillin" CDS 1634..2494 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2649..3268 /label="pBR322_origin" ORIGIN 1 GTTAACCTGG CTTATCGAAA TTAATACGAC TCACTATAGG GAGACCGGCA GATCTGATAT 61 CATCGATGAA TTCGAGCTcc accgcggtgg cggccgctct agaactagtg gatcccccgg 121 gcATTTGTAT AGTTCATCCA TGCCATGTGT AATCCCAGCA GCTGTTACAA ACTCAAGAAG 181 GACCATGTGG TCTCTCTTTT CGTTGGGATC TTTCGAAAGG GCAGATTGTG TGGACAGGTA 241 ATGGTTGTCT GGTAAAAGGA CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC 301 TGCTAGTTGA ACGCTTCCAT CTTCAATGTT GTGTCTAATT TTGAAGTTAA CTTTGATTCC 361 ATTCTTTTGT TTGTCTGCCA TGATGTATAC ATTGTGTGAG TTATAGTTGT ATTCCAATTT 421 GTGTCCAAGA ATGTTTCCAT CTTCTTTAAA ATCAATACCT TTTAACTCGA TTCTATTAAC 481 AAGGGTATCA CCTTCAAACT TGACTTCAGC ACGTGTCTTG TAGTTCCCGT CATCTTTGAA 541 AAATATAGTT CTTTCCTGTA CATAACCTTC GGGCATGGCA CTCTTGAAAA AGTCATGCcG 601 TTTCATATGA TCTGGGTATC TTGAAAAGCA TTGAACACCA TAAGAGAAAG TAGTGACAAG 661 TGTTGGCCAT GGAACAGGTA GTTTTCCAGT AGTGCAAATA AATTTAAGGG TAAGTTTTCC 721 GTATGTTGCA TCACCTTCAC CCTCTCCACT GACAGAAAAT TTGTGCCCAT TAACATCACC 781 ATCTAATTCA ACAAGAATTG GGACAACTCC AGTGAAAAGT TCTTCTCCTT TACTCATTTT 841 TTCTACCGGT ACCCAATTCG CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG 901 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC 961 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC 1021 AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA 1501 TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 1561 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 1621 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 1681 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA 1741 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 1801 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG 1861 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 1921 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 1981 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT 2041 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 2101 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 2161 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 2221 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 2281 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG 2341 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 2401 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 2461 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 2521 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT 2581 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 2641 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 2701 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 2761 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 2821 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 2881 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 2941 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3001 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3061 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3121 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3181 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3241 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3301 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC 3361 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 3421 CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 3481 TTAATGCAG // 1650 LOCUS ADDGENE_1650 5004 bp DNA circular SYN 01/03/2005 DEFINITION L4431 complete plasmid sequence KEYWORDS . SOURCE L4431 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1650 at www.addgene.org FEATURES Location/Qualifiers source 1..5004 /organism="Addgene plasmid L4431" /mol_type="other DNA" CDS 1..1842 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MTMITPSLHACKLALIRLTIGRNCRSTLEDPRVPVEKMSKGEEL FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFS YGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRI ELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADH YQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKC PGDPLVLERPPLEVDGIDKLDIEFGRWVPCAKVKDTKAHIDGLKKGQTYQFRVKAVNK EGASDALSTDKDTKAKNPYDEPGKTGTPDVVDWDADRVSLEWEPPKSDGGAPITQYVI EKKGKHGRDWQECGKVSGDQTNAEILGLKEGEEYQFRVKAVNKAGPGEASDPSRKVVA KPRNLKPWIDREAMKTITIKVGNDVEFDVPVRGEPPPKKEWIFNEKPVDDQKIRIESE DYKTRFVLRGATRKHAGLYTLTATNASGSDKHSVEVIVLGGDPLVLERPPLEVDGIDK LDIEFYECYLYSSSMPCVIPAAVTNSRRTMWSLFSLGSFERADCVDR" promoter 45..63 /label="T7_promoter" gene 112..819 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 295..318 /label="gfp.S65S" misc_feature complement(2232..2255) /label="gfp.S65S" gene complement(1734..2438) /label="GFP(variant)" /gene="GFP(variant)" CDS complement(1722..2438) /label="GFP(variant)" /gene="GFP(variant)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK" terminator 2489..2524 /label="T7_terminator" misc_feature 2489..2536 /label="Tpol.3'" promoter 2918..2946 /label="Amp_promoter" gene 2988..3848 /label="Ampicillin" /gene="Ampicillin" CDS 2988..3848 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4003..4622 /label="pBR322_origin" misc_feature 4838..4864 /label="lacI.C" promoter 4931..4960 /label="lac_promoter" misc_feature 4995..5013 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAagctag ccctaatacg actcactata 61 gggcgaaact gcaGGTCGAC TCTAGAGGAT CCCCGGGTAC CGGTAGAAAA AATGAGTAAA 121 GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT 181 GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC 241 CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGCCAACACT TGTCACTACT 301 TTCTCTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC ATATGAAACg GCATGACTTT 361 TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA CTATATTTTT CAAAGATGAC 421 GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATAGAATC 481 GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA ATTGGAATAC 541 AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT 601 AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA 661 CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA 721 CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT 781 GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATgccc gggggatcca 841 ctagttctag agcggccccc cctcgaggtc gacggtatcg ataagcttga tatcgAATTC 901 GGAAGATGGG TTCCATGCGC CAAAGTTAAG GATACGAAGG CTCATATTGA TGGTCTCAAG 961 AAGGGACAAA CATATCAGTT CCGTGTCAAG GCTGTCAATA AGGAAGGAGC TTCTGATGCA 1021 TTGTCTACTG ATAAGGACAC CAAAGCCAAG AATCCATATG ATGAGCCAGG AAAAACCGGA 1081 ACTCCGGATG TTGTCGACTG GGATGCTGAT CGTGTCTCAC TTGAATGGGA ACCACCAAAG 1141 TCTGACGGAG GAGCTCCAAT CACTCAATAC GTCATTGAGA AGAAGGGCAA ACATGGAAGA 1201 GACTGGCAAG AATGCGGAAA GGTTTCTGGA GATCAAACCA ATGCTGAGAT TCTTGGACTC 1261 AAGGAGGGAG AAGAGTACCA GTTCCGTGTG AAGGCTGTTA ACAAGGCCGG ACCGGGAGAG 1321 GCTTCAGACC CAAGCCGAAA GGTTGTTGCA AAGCCAAGAA ACTTGAAGCC ATGGATTGAT 1381 CGTGAAGCAA TGAAGACGAT CACTATCAAG GTAGGAAACG ATGTGGAATT CGATGTTCCA 1441 GTACGCGGAG AACCACCACC GAAGAAGGAA TGGATCTTCA ATGAGAAACC AGTCGATGAT 1501 CAAAAGATCA GGATTGAAAG CGAAGACTAC AAGACCCGAT TTGTGCTCCG TGGAGCAACT 1561 CGCAAGCATG CTGGTTTGTA CACTCTTACT GCTACCAACG CTTCTGGAAG CGACAAACAT 1621 TCCGTTGAGG TCATTGTGCT CGGgggggat ccactagttc tagagcGGCC CCCCCTCGAG 1681 GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTACGAAT GCTATTTGTA TAGTTCATCC 1741 ATGCCATGTG TAATCCCAGC AGCTGTTACA AACTCAAGAA GGACCATGTG GTCTCTCTTT 1801 TCGTTGGGAT CTTTCGAAAG GGCAGATTGT GTGGACAGGT AATGGTTGTC TGGTAAAAGG 1861 ACAGGGCCAT CGCCAATTGG AGTATTTTGT TGATAATGGT CTGCTAGTTG AACGCTTCCA 1921 TCTTCAATGT TGTGTCTAAT TTTGAAGTTA ACTTTGATTC CATTCTTTTG TTTGTCTGCC 1981 ATGATGTATA CATTGTGTGA GTTATAGTTG TATTCCAATT TGTGTCCAAG AATGTTTCCA 2041 TCTTCTTTAA AATCAATACC TTTTAACTCG ATTCTATTAA CAAGGGTATC ACCTTCAAAC 2101 TTGACTTCAG CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT TCTTTCCTGT 2161 ACATAACCTT CGGGCATGGC ACTCTTGAAA AAGTCATGCc GTTTCATATG ATCTGGGTAT 2221 CTTGAAAAGC ATTGAACACC ATAAGAGAAA GTAGTGACAA GTGTTGGCCA TGGAACAGGT 2281 AGTTTTCCAG TAGTGCAAAT AAATTTAAGG GTAAGTTTTC CGTATGTTGC ATCACCTTCA 2341 CCCTCTCCAC TGACAGAAAA TTTGTGCCCA TTAACATCAC CATCTAATTC AACAAGAATT 2401 GGGACAACTC CAGTGAAAAG TTCTTCTCCT TTACTCATTT TTTCTAccGG GGGATCCACT 2461 AGTTCTAGAG CGGCCGTACG GGCCgatcat aaccccttgg ggcctctaaa cgggtcttga 2521 ggggtttttt ttaattgggc cCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 2581 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 2641 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 2701 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 2761 CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT 2821 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG 2881 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 2941 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 3001 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 3061 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 3121 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 3181 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 3241 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 3301 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 3361 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 3421 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 3481 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 3541 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 3601 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 3661 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 3721 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 3781 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 3841 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 3901 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 3961 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 4021 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 4081 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 4141 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 4201 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 4261 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 4321 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 4381 ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA 4441 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 4501 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 4561 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 4621 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 4681 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 4741 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 4801 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 4861 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 4921 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 4981 AACAATTTCA CACAGGAAAC AGCT // 1651 LOCUS ADDGENE_1651 4784 bp DNA circular SYN 01/03/2005 DEFINITION L4433 complete plasmid sequence KEYWORDS . SOURCE L4433 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1651 at www.addgene.org FEATURES Location/Qualifiers source 1..4784 /organism="Addgene plasmid L4433" /mol_type="other DNA" promoter 45..63 /label="T7_promoter" gene 792..1163 /label="GFP(336-708)" /gene="GFP(336-708)" CDS complement(56..862) /label="Orf1" /translation="MFPSSLKSIPFNSILLTRVSPSNLTSAPPIQGYVIEKREVGSTE WTKAAFGNILDTKHRVTGLTPKKTYEFRVAAYNAAGQGEYSVNSVPITADNAPTRPKI NMGMLTRDILAYAGERAKILVPFAASPAPKVTFSKGENKISPTDPRVKVEYSDFLATL TIEKSELTDGGLYFVELENSQGSDSASIRLKVVDKPASPQHIRVEDIAPDCCTLYWMP PSSDGGSPITNYIVEKLGCRNSISSLSIPSTSRGGPVPGDPLESTCSFAL" CDS 1147..2289 /label="Orf0" /translation="MAWMNYTNSIRRIPAAQLNVFVLDAPGKPTGPIRATDIQADAMT LSWRPPKDNGGDAITNYVVEKRTPGGDWVTVGHPVGTTLRVRNLDANTPYEFRVRAEN QYGVGEPLETDDAIVAKNPFDTPGAPGQPEAVETSEEAITLQWTRPTSDGGAPIQGYV IEKREVGSTEWTKAAFGNILDTKHRVTGLTPKKTYEFRVAAYNAAGQGEYSVNSVPIT ADNAPTRPKINMGMLTRDILAYAGERAKILVPFAASPAPKVTFSKGENKISPTDPRVK VEYSDFLATLTIEKSELTDGGLYFVELENSQGSDSASIRLKVVDKPASPQHIRVEDIA PDCCTLYWMPPSSDGGSPITNYIVEKLGDPLVLERPYGPIITPWGL" terminator 2269..2304 /label="T7_terminator" misc_feature 2269..2316 /label="Tpol.3'" promoter 2698..2726 /label="Amp_promoter" gene 2768..3628 /label="Ampicillin" /gene="Ampicillin" CDS 2768..3628 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3783..4402 /label="pBR322_origin" misc_feature 4618..4644 /label="lacI.C" promoter 4711..4740 /label="lac_promoter" misc_feature 4775..4793 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAagctag ccctaatacg actcactata 61 gggcgaaact gcaGGTCGAC TCTAGAGGAT CCCCGGGTAC CGGGCCCCCC CTCGAGGTCG 121 ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGCCCAG CTTCTCAACG ATGTAGTTTG 181 TGATTGGCGA TCCTCCATCA GATGATGGTG GCATCCAGTA AAGAGTGCAG CAGTCTGGTG 241 CAATATCCTC TACTCGGATG TGTTGTGGTG AAGCTGGTTT ATCAACAACC TTCAATCTGA 301 TACTAGCAGA ATCACTTCCT TGACTATTTT CAAGTTCAAC AAAGTAGAGT CCACCATCAG 361 TAAGTTCAGA CTTCTCGATC GTCAATGTAG CCAAGAAGTC ACTGTACTCA ACCTTCACTC 421 TTGGATCAGT TGGGCTGATC TTATTCTCAC CCTTCGAGAA AGTGACTTTC GGAGCAGGTG 481 AAGCAGCAAA TGGAACAAGA ATCTTTGCAC GTTCTCCAGC GTAAGCGAGG ATATCGCGAG 541 TAAGCATTCC CATGTTGATC TTCGGTCTTG TTGGAGCATT ATCAGCTGTA ATTGGAACAG 601 AGTTAACACT GTATTCTCCT TGTCCAGCTG CATTGTAAGC TGCAACTCTG AATTCGTAGG 661 TCTTCTTTGG AGTGAGTCCA GTGACACGAT GTTTTGTGTC AAGGATGTTT CCAAATGCAG 721 CTTTCGTCCA TTCAGTTGAT CCGACTTCTC TCTTCTCAAT CACATATCCT TGAATTGGAG 781 GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA AAAGGTATTG 841 ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT AACTCACACA 901 ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTAACTTC AAAATTAGAC 961 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 1021 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 1081 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1141 TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTCCTGC AGCCCAGCTT 1201 AATGTCTTCG TTCTTGATGC TCCAGGAAAA CCAACTGGAC CAATCCGTGC TACTGACATT 1261 CAAGCCGATG CAATGACGCT TTCATGGAGA CCACCAAAGG ATAATGGAGG AGATGCTATC 1321 ACCAATTATG TCGTTGAAAA GAGAACTCCA GGCGGTGACT GGGTCACTGT CGGACATCCT 1381 GTTGGAACAA CTCTTCGTGT CCGCAATTTG GATGCCAATA CTCCTTACGA ATTCCGTGTT 1441 CGCGCTGAAA ATCAATATGG AGTTGGAGAG CCACTTGAAA CTGATGACGC AATTGTTGCC 1501 AAGAATCCGT TCGATACTCC AGGAGCTCCA GGACAACCAG AAGCCGTTGA AACTTCGGAG 1561 GAAGCAATTA CACTTCAATG GACAAGACCA ACATCTGATG GAGGAGCTCC AATTCAAGGA 1621 TATGTGATTG AGAAGAGAGA AGTCGGATCA ACTGAATGGA CGAAAGCTGC ATTTGGAAAC 1681 ATCCTTGACA CAAAACATCG TGTCACTGGA CTCACTCCAA AGAAGACCTA CGAATTCAGA 1741 GTTGCAGCTT ACAATGCAGC TGGACAAGGA GAATACAGTG TTAACTCTGT TCCAATTACA 1801 GCTGATAATG CTCCAACAAG ACCGAAGATC AACATGGGAA TGCTTACTCG CGATATCCTC 1861 GCTTACGCTG GAGAACGTGC AAAGATTCTT GTTCCATTTG CTGCTTCACC TGCTCCGAAA 1921 GTCACTTTCT CGAAGGGTGA GAATAAGATC AGCCCAACTG ATCCAAGAGT GAAGGTTGAG 1981 TACAGTGACT TCTTGGCTAC ATTGACGATC GAGAAGTCTG AACTTACTGA TGGTGGACTC 2041 TACTTTGTTG AACTTGAAAA TAGTCAAGGA AGTGATTCTG CTAGTATCAG ATTGAAGGTT 2101 GTTGATAAAC CAGCTTCACC ACAACACATC CGAGTAGAGG ATATTGCACC AGACTGCTGC 2161 ACTCTTTACT GGATGCCACC ATCATCTGAT GGAGGATCGC CAATCACAAA CTACATCGTT 2221 GAGAAGCTGG GGGATCCACT AGTTCTAGAG CGGCCGTACG GGCCgatcat aaccccttgg 2281 ggcctctaaa cgggtcttga ggggtttttt ttaattgggc cCTTTCGTCT CGCGCGTTTC 2341 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 2401 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 2461 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 2521 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 2581 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 2641 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 2701 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 2761 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 2821 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 2881 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 2941 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 3001 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 3061 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 3121 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 3181 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 3241 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 3301 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 3361 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 3421 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 3481 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 3541 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 3601 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 3661 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 3721 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3781 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3841 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3901 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 3961 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 4021 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 4081 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 4141 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 4201 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 4261 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 4321 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 4381 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4441 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 4501 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 4561 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 4621 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 4681 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 4741 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1652 LOCUS ADDGENE_1652 6588 bp DNA circular SYN 01/03/2005 DEFINITION L4437 complete plasmid sequence KEYWORDS . SOURCE L4437 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1652 at www.addgene.org FEATURES Location/Qualifiers source 1..6588 /organism="Addgene plasmid L4437" /mol_type="other DNA" misc_feature 62..87 /label="pes10.UAS-270" misc_feature 309..331 /label="pes10.Tsa" misc_feature 371..412 /label="SynIVS.A" misc_feature 494..520 /label="SV40.NLS" CDS 494..3238 /label="Orf1" /translation="MTAPKKKRKVPVEKMNTINIAKNDFSDIELAAIPFNTLADHYGE RLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDW FEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIE DEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSW HKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALA GISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAI NIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWK RAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQG NDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAK SPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRD EVGGRAVVSLNSMLLTND*HLNFQNLLPSETVQDIYGIVAKKVNEILQADAINGTDNE VVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVL EDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAE VKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDS EIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLF KAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA" misc_feature 536..565 /label="T7pol.N" misc_feature 3203..3235 /label="T7pol.C" misc_feature 3324..3374 /label="SynIVS.L" promoter 4502..4530 /label="Amp_promoter" CDS 4572..5432 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4572..5432 /label="Ampicillin" /gene="Ampicillin" rep_origin 5587..6206 /label="pBR322_origin" misc_feature 6422..6448 /label="lacI.C" promoter 6515..6544 /label="lac_promoter" misc_feature 6579..6597 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGtcga ctctagagga tccccgggta 61 cctaatttga tcgataatat gtacatattt tgttattcgt tccggtatcg tacctccacc 121 taccataaat cccataccct acatctttAT CGATTTTTTG CAAATTACGA GCGTTGTAGG 181 GGGCGGAgCG ATAGGTCCTA TAGGTTTTgG TATATCATCA TTCATTCATT CATTGGTACA 241 TTCATTTACC CACCTTCCTC TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT 301 TATCTTTATA CTGTAATTTT TAACTTTCAG gcattgattg gatccccggg attggccaaa 361 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc 421 ttggCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT GGAGGGTACC 481 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG AAAAAATGAA 541 CACGATTAAC ATCgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa 601 cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga 661 gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg 721 tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat 781 tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc 841 cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac 901 tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg 961 tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt 1021 caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt 1081 tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc 1141 ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga 1201 gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga 1261 gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc 1321 tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac 1381 tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa 1441 gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat 1501 tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac 1561 caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat 1621 gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc 1681 tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct 1741 tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg 1801 gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg 1861 actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat 1921 ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat 1981 tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg 2041 ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca 2101 gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg 2161 catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttGTAAGTTT 2221 AAACTCTATG CTACTAACTA ACGATTAaCA TTTAAATTTT CAGaacttgc ttcctagtga 2281 aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga 2341 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat 2401 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt 2461 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg 2521 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct 2581 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt 2641 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct 2701 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt 2761 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac 2821 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa 2881 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag 2941 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga 3001 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt 3061 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt 3121 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc 3181 ggctaaaggt aacttgaacc tccgtgacat cttagagtcG GACTTCGCGT TCGCGTAGCA 3241 TTCGTAGAAT TCCAACTGAG CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA 3301 TAATAGGGGC CGCTGTCATC AGAgtaagtt taaactgagt tctactaact aacgagtaat 3361 atttaaattt tcagCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA 3421 CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA 3481 CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG 3541 TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC 3601 CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT 3661 TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT 3721 CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC 3781 ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC 3841 AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT 3901 TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT 3961 GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA 4021 TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC 4081 AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG 4141 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 4201 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 4261 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 4321 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG 4381 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 4441 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 4501 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 4561 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 4621 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 4681 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 4741 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 4801 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 4861 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 4921 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 4981 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 5041 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 5101 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 5161 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 5221 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 5281 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 5341 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 5401 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 5461 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 5521 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 5581 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 5641 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 5701 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT 5761 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 5821 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 5881 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 5941 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG 6001 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 6061 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 6121 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 6181 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 6241 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 6301 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 6361 AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT 6421 AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA 6481 ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 6541 TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1653 LOCUS ADDGENE_1653 6591 bp DNA circular SYN 01/03/2005 DEFINITION L4438 complete plasmid sequence KEYWORDS . SOURCE L4438 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1653 at www.addgene.org FEATURES Location/Qualifiers source 1..6591 /organism="Addgene plasmid L4438" /mol_type="other DNA" misc_feature 62..87 /label="pes10.UAS-270" misc_feature 309..331 /label="pes10.Tsa" misc_feature 371..412 /label="SynIVS.A" CDS 494..3406 /label="Orf1" /translation="MTAPKKKRKVPVEKMNIIENIEKNDFSEIELAAIPFNTLADHYG SALAKEQLALEHESYELGERRFLKMLERQAKAGEIADNAAAKPLLATLLPKLTTRIVE WLEEYASKKGRKPSAYAPLQLLKPEASAFITLKVILASLTSTNMTTIQAAAGMLGKAI EDEARFGRIRDLEAKHFKKHVEEQLNKRHGQVYKKAFMQVVEADMIGRGLLGGEAWSS WDKETTMHVGIRLIEMLIESTGLVELQRHNAGNAGSDHEALQLAQEYVDVLAKRAGAL AGISPMFQPCVVPPKPWVAITGGGYWANGRRPLALVRTHSKKGLMRYEDVYMPEVYKA VNLAQNTAWKINKKVLAVVNEIVNWKNCPVADIPSLERQELPPKPDDIDTNEAALKEW KKAAAGIYRLDKARVSRRISLEFMLEQANKFASKKGKFKLGSTN*RIHLNFSAIWFPY NMDWRGRVYAVPMFNPQGNDMTKGLLTLAKGKPIGEEGFYWLKIHGANCAGVDKVPFP ERIAFIEKHVDDILACAKDPINNTWWAEQDSPFCFLAFCFEYAGVTHHGLSYNCSLPL AFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAQKVNEILKQDAINGTPN EMITVTDKDTGEISEKLKLGTSTLAQQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQV LDDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWDAVSVTVVAAVEAMNWLKSAAKLLAA EVKDKKTKEILRHRCAVHWTTPDGFPVWQEYRKPLQKRLDMIFLGQFRLQPTINTLKD SGIDAHKQESGIAPNFVHSQDGSHLRMTVVYAHEKYGIESFALIHDSFGTIPADAGKL FKAVRETMVITYENNDVLADFYSQFADQLHETQLDKMPPLPKKGNLNLQDILKSDFAF A*HS*NSN*APVATITNLSGVKNNRGRCHQSKFKLSSTN*RVIFKFSASRARASDF" misc_feature 494..520 /label="SV40.NLS" misc_feature 3327..3377 /label="SynIVS.L" promoter 4505..4533 /label="Amp_promoter" CDS 4575..5435 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4575..5435 /label="Ampicillin" /gene="Ampicillin" rep_origin 5590..6209 /label="pBR322_origin" misc_feature 6425..6451 /label="lacI.C" promoter 6518..6547 /label="lac_promoter" misc_feature 6582..6600 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGtcga ctctagagga tccccgggta 61 cctaatttga tcgataatat gtacatattt tgttattcgt tccggtatcg tacctccacc 121 taccataaat cccataccct acatctttAT CGATTTTTTG CAAATTACGA GCGTTGTAGG 181 GGGCGGAgCG ATAGGTCCTA TAGGTTTTgG TATATCATCA TTCATTCATT CATTGGTACA 241 TTCATTTACC CACCTTCCTC TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT 301 TATCTTTATA CTGTAATTTT TAACTTTCAG gcattgattg gatccccggg attggccaaa 361 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc 421 ttggCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT GGAGGGTACC 481 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG AAAAAATGAA 541 CATCATCGAA AACATCGAAA AGAATGACTT CTCAGAAATC GAACTGGCTG CTATCCCGTT 601 CAACACACTG GCTGACCACT ACGGAAGCGC CTTGGCTAAA GAGCAGTTGG CTTTAGAACA 661 TGAGTCTTAT GAGCTAGGCG AGCGCCGCTT CCTCAAGATG CTTGAGCGTC AAGCGAAAGC 721 TGGTGAGATT GCAGACAACG CAGCCGCTAA GCCGTTACTC GCTACGCTTC TCCCTAAGTT 781 AACCACACGT ATCGTCGAGT GGCTCGAAGA GTACGCATCG AAGAAAGGCC GCAAGCCTAG 841 CGCATACGCA CCGCTCCAGT TACTCAAGCC GGAGGCCTCC GCGTTTATCA CCCTGAAAGT 901 TATCCTTGCG TCACTAACCA GTACGAACAT GACAACCATT CAGGCCGCTG CTGGTATGCT 961 GGGGAAAGCC ATTGAGGACG AGGCACGATT TGGGCGCATC CGTGACCTAG AAGCGAAGCA 1021 CTTCAAGAAG CACGTTGAGG AACAGCTTAA CAAGCGCCAC GGGCAAGTCT ACAAGAAAGC 1081 ATTTATGCAG GTGGTCGAGG CCGATATGAT TGGTCGAGGT CTGCTTGGTG GCGAGGCGTG 1141 GTCTAGCTGG GATAAAGAAA CCACGATGCA CGTAGGGATT CGCCTGATTG AAATGCTGAT 1201 TGAATCCACG GGTCTGGTGG AATTACAGCG CCACAACGCA GGTAACGCAG GCTCTGACCA 1261 TGAGGCACTG CAACTGGCCC AAGAGTACGT GGACGTATTA GCGAAGCGTG CAGGCGCTCT 1321 GGCGGGTATC TCTCCGATGT TCCAGCCGTG TGTCGTACCG CCGAAACCTT GGGTAGCAAT 1381 CACAGGGGGC GGCTATTGGG CTAACGGTCG CAGACCTTTG GCACTCGTTC GCACTCACTC 1441 TAAGAAGGGC TTGATGCGCT ACGAAGACGT TTACATGCCA GAAGTCTACA AGGCTGTGAA 1501 CCTCGCGCAA AACACCGCAT GGAAAATCAA CAAGAAAGTT CTTGCTGTTG TCAATGAGAT 1561 TGTTAACTGG AAGAATTGCC CGGTAGCAGA CATTCCATCG CTGGAGCGCC AAGAGTTACC 1621 GCCTAAGCCT GACGACATTG ACACCAACGA GGCAGCGCTC AAGGAGTGGA AGAAAGCCGC 1681 TGCTGGTATC TATCGCTTGG ACAAGGCACG AGTGTCTCGC CGTATCAGCT TAGAGTTCAT 1741 GCTGGAGCAG GCCAACAAGT TCGCAAGcAA GAAAGgtaag tttaaacttg gatctactaa 1801 ctaacgaatc catttaaatt tttcagCAAT CTGGTTCCCT TACAACATGG ACTGGCGCGG 1861 TCGTGTGTAC GCTGTGCCGA TGTTCAACCC GCAAGGCAAC GACATGACGA AAGGTCTGCT 1921 GACCCTTGCT AAAGGCAAGC CAATCGGTGA GGAAGGTTTC TACTGGCTGA AAATCCACGG 1981 TGCGAACTGT GCGGGTGTTG ATAAGGTTCC ATTCCCGGAG CGCATCGCGT TCATTGAGAA 2041 GCACGTAGAC GACATTCTGG CTTGCGCTAA AGACCCAATC AATAACACTT GGTGGGCTGA 2101 GCAGGATTCA CCGTTCTGTT TCCTCGCGTT TTGCTTCGAG TATGCAGGCG TTACGCACCA 2161 CGGTCTGAGC TACAATTGCT CTCTGCCGCT GGCGTTCGAC GGGTCTTGCT CTGGTATCCA 2221 GCACTTCTCC GCGATGCTCC GCGATGAGGT AGGCGGTCGT GCGGTTAACC TGCTGCCAAG 2281 CGAAACCGTG CAGGACATTT ACGGCATCGT TGCACAGAAA GTAAACGAGA TTCTCAAACA 2341 GGATGCAATC AACGGCACGC CTAACGAGAT GATTACCGTG ACCGACAAGG ACACCGGGGA 2401 AATCTCAGAG AAGCTCAAAC TTGGAACCTC AACGCTGGCG CAACAGTGGC TGGCATATGG 2461 TGTAACCCGT AGCGTAACTA AACGTTCGGT CATGACGCTG GCTTACGGTT CCAAGGAGTT 2521 CGGCTTTCGT CAACAGGTAT TGGATGACAC CATTCAGCCT GCAATTGACA GCGGTAAGGG 2581 CTTGATGTTC ACCCAACCGA ACCAAGCGGC TGGCTATATG GCTAAGCTGA TTTGGGATGC 2641 GGTAAGCGTG ACCGTAGTTG CAGCGGTTGA GGCGATGAAC TGGCTCAAAT CTGCCGCTAA 2701 GCTGCTGGCT GCTGAGGTCA AGGACAAGAA GACCAAGGAG ATTCTGCGCC ACCGTTGCGC 2761 GGTTCACTGG ACTACGCCGG ACGGCTTCCC GGTCTGGCAG GAATACCGCA AGCCACTCCA 2821 GAAGCGTCTC GATATGATTT TCTTAGGGCA ATTCCGTCTG CAACCGACGA TTAATACCCT 2881 CAAGGATTCA GGCATTGACG CACACAAGCA GGAGTCTGGC ATCGCTCCTA ACTTTGTTCA 2941 CTCACAGGAC GGTAGCCACC TCCGCATGAC AGTCGTTTAT GCTCACGAGA AGTATGGCAT 3001 TGAGTCCTTT GCGCTCATCC ATGACAGCTT TGGGACTATC CCGGCAGACG CTGGTAAGCT 3061 CTTTAAGGCT GTGCGTGAAA CGATGGTTAT CACCTATGAG AACAACGATG TGCTGGCAGA 3121 CTTCTACTCT CAGTTTGCCG ACCAGCTACA CGAGACCCAA CTGGACAAGA TGCCTCCGCT 3181 TCCGAAGAAA GGAAACCTGA ACCTGCAAGA CATTCTCAAG TCTGACTTTG CCTTTGCATA 3241 GCATTCGTAG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA 3301 AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt 3361 aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT 3421 CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA 3481 AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT 3541 GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC 3601 CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG 3661 TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT 3721 TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT 3781 CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA 3841 ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT 3901 GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC 3961 GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT 4021 TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT 4081 CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC 4141 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 4201 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 4261 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 4321 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA 4381 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA 4441 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 4501 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 4561 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 4621 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 4681 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 4741 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG 4801 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 4861 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 4921 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 4981 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 5041 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 5101 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 5161 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 5221 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 5281 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 5341 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 5401 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 5461 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 5521 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 5581 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 5641 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 5701 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 5761 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 5821 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 5881 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 5941 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT 6001 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 6061 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 6121 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 6181 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 6241 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 6301 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 6361 GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC 6421 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA 6481 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 6541 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1654 LOCUS ADDGENE_1654 2790 bp DNA circular SYN 05/14/2005 DEFINITION L4440 complete plasmid sequence KEYWORDS . SOURCE L4440 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1654 at www.addgene.org FEATURES Location/Qualifiers source 1..2790 /organism="Addgene plasmid L4440" /mol_type="other DNA" promoter 20..38 /label="T7_promoter" promoter complement(222..240) /label="T7_promoter" misc_feature 260..408 /label="lacZ_a" misc_feature complement(251..267) /label="M13f20_primer" rep_origin 426..732 /label="f1_origin" promoter 925..953 /label="Amp_promoter" gene 995..1855 /label="Ampicillin" /gene="Ampicillin" CDS 995..1855 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2010..2629 /label="pBR322_origin" ORIGIN 1 AACCTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCGGCAGAT CTGATATCAT 61 CGATGAATTC GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTggat ccaccggttc 121 catggctagc cacgtgacgc gtggatccCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT 181 CGATACCGTC GACCTCGAGG GGGGGCCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT 241 ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 301 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 361 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA 421 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 481 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 541 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 601 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 661 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 721 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 781 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA 841 ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 901 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 961 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC 1021 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT 1081 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT 1141 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC 1201 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT 1261 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA 1321 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA 1381 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT 1441 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA 1501 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG 1561 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT 1621 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT 1681 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC 1741 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA 1801 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC 1861 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG 1921 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 1981 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 2041 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 2101 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 2161 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 2221 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 2281 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 2341 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 2401 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 2461 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 2521 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 2581 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 2641 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 2701 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 2761 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC // 1655 LOCUS ADDGENE_1655 8153 bp DNA circular SYN 01/03/2005 DEFINITION L4453 complete plasmid sequence KEYWORDS . SOURCE L4453 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1655 at www.addgene.org FEATURES Location/Qualifiers source 1..8153 /organism="Addgene plasmid L4453" /mol_type="other DNA" misc_feature 48..78 /label="rps5.-4.0k" misc_feature 3968..4001 /label="rps5.5'" CDS 4041..5117 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 4041..4067 /label="SV40.NLS" misc_feature 4083..4109 /label="SV40.NLS" misc_feature 4125..4151 /label="SV40.NLS" misc_feature 4167..4193 /label="SV40.NLS" gene 4209..5057 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 4380..4430 /label="SynIVS.R" misc_feature 4452..4471 /label="gfp.S65C" misc_feature 4585..4635 /label="SynIVS.S" misc_feature 4800..4850 /label="SynIVS.T" misc_feature 5223..5252 /label="let858.3'" promoter 5933..5961 /label="Amp_promoter" gene 6003..6863 /label="Ampicillin" /gene="Ampicillin" CDS 6003..6863 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7018..7637 /label="pBR322_origin" misc_feature 7853..7879 /label="lacI.C" promoter 7946..7975 /label="lac_promoter" misc_feature 8010..8028 /label="M13r_primer" misc_feature 8042..8092 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGTTCCA AAAAAGCGTG AGCAAACGAA TAGACAGata tttttgaact 61 atgattcatg atggtgactg atggtcagct tatcatcacg aacctgatga tcggaagaaa 121 agacaacatt ttaccaaata gaatctgcat atttaaatat ctagataatt tttttggggg 181 ctctaaacta tttgggagag tgcaagacag tgaaaataat ttatatttta tactattttt 241 tcgaagtctc aatattatag ctcaatttaa tataattcca aagcaaaaat tagatttaat 301 aaaactgaag taaaaagtca tcggggaaaa ttccaccaac tgcgatatga aatattagtt 361 ttgtaaaaca aagttcaata aaataataat gtagcctaac gtatgaaaca ttccaaattc 421 agaattgggt ttctatacct aaatttcaaa aagtattttt cagatcaagc tattcaaaat 481 gatttcacta tgacagaaat gtatgttgca aatttgaaaa aaagattttg gatatatttt 541 ttgaagtact ttgttgctac atagatcttc taaaaaatta aattaaaaga aaaatgtatt 601 tacgctatga ttttattaaa ctgctaaaat agattccaaa tttggtactt tccaaagtgg 661 ttcgataaaa tgaaacatct aaatttaaat acatatgaat caaaatttta aatgcaaata 721 aacaacttca aatatagaat caaaaagcaa gttatttgtt tggaaagcat ttttcaatca 781 tcaatccaat aagaaagtgc caatgaaatt attttggaat cgcttgaact attttcagac 841 aaattcactg cttacatcat tttatagggt tataccaaaa tgcaaggcat acatcatttt 901 gaataacttt ctttagaaat tttcatttca agataaatta tagttctaaa aatttgattt 961 caccctactg ctttccaact atacagttac aaagtctgaa gggttaagtg taaaaaaacg 1021 aaacggtaac tgtgaagaat tcaatattct tcgtctaaat attgtttgac aatgaattct 1081 gttcacttga tgattcacat atttttgttt tggatttata tattctcact tgaagaagta 1141 tagtacacta tctggctgtt tgccaactgg gagggacagg aaattgcaac gatttgatca 1201 tttatttgtt tcgattctga atcatcttca tcatatttag tttgagcatt tgaatattct 1261 gatgggaagt aatcatatta atttgtcaca tttaaattgc aagtagattt ggaaagtatt 1321 tttgcataag aatattttcg tgtagaattt ccagagtatt catgtatgtg gtcattattt 1381 taaacattac tcatatttat aaagtaaaca aatattgaca attcccaatg atttgaaaat 1441 gaggtagatg tcaaaaaagg aaggcatttc ggaatcaaat atctcgatcc gactgaaact 1501 tgttctattt aaggacgtca aaactgtgtg cgcctttgaa gagtactgta cttcttcact 1561 ttttattcct tccaaatcta aatgaatgct tgaactttca aaataaacat aaaactcttg 1621 agagaaaacg aatggaaaaa ttggaagaaa ttaagaatca tcaggtagct gctacagtac 1681 tctctgaagg cgcatacacg tttgctcgaa tttcatatga caaaatttta ggtcctgtcg 1741 agaccggtta catgactttt acatagaaca attcaaatat gaaatgtgga attgctatct 1801 catttggaag agcttaaaca caattttcta actttaatat gatttggaaa taagagcaaa 1861 tgtagaatat ttaattttgg aaaactctta ctttaatctt tttgtgtatt ctccgtttct 1921 tcagattgtc ttcttgatgc cgcgtaacaa tgtttctaga attagatgca ataactcgtg 1981 gatctctcga tccaattgat cccaactcta tagattcaga ggaataggaa attgtagaga 2041 caggcattgc agaaaaaaac aatgagaaca gaatgcaaaa gctctctgat gttttgtgtt 2101 ggtcataaaa atgataaaat aacaatagaa atcagcttgg gaacccgtgg gcgctcccca 2161 ttttattgtt tgggaactga gaaaatggcc gtaaattgtt tttggaaggt tatggtatga 2221 cagggtttct ggctcaaatg taaagtctta aatttacttg gagttttcaa atgtgtcaat 2281 cttttggtaa aaatatattt tttttaaatc atgcatcaag aaagggttgg gaaatcttca 2341 aattacagta ccacgaaatg ggatgaggtt tatatagtta tcctatggag tttgtagttt 2401 tgaaaggttc caaattttac aaaaattgta tgcaggcacg ccacgccttc gtatctgcct 2461 gcctgcctgc ccaccggtta cattccgtgt tttggcatga aattcgattt ttagctattt 2521 ttattttgtc ttttcaaaaa ccttttaaaa gtttcatgaa gtaataaata caaaaaaaat 2581 gtgaatactg aggttcagat gaactgtggt acataggcac acgtgtaggt acattgcagg 2641 caatttatta ttaacttcaa tatgtttatg attacaatta ccaatacaag tatatcaatc 2701 aagtactata tagttgagat tcaaaaattt ccaaaaagta agttctacga gaaacataaa 2761 agttcagatt cttcgtggac agaaaaagaa aacgaagaag aagatgagcc agaaagtaat 2821 aattggaaat cgaaaactaa acatttttgg accgactctc tgtgtatttt agagtgtcac 2881 gggcatggga taaattgtga tgctctcttc agagcacaag tgtgacgagc acacgcacga 2941 caactgacgt ggcaaatgga tgaaaacgaa gaaaagaaat tagaaaagcc acacgaatca 3001 tgtcggtgaa gtgagaggaa aacgacaaaa tattgtgatt tattgatttg tggatagaag 3061 aaaactcgta actttataaa agactattta aagttatcaa gaaaatttta taataaaatc 3121 agaggtttgc gcttgaagaa gatacgtata tcaattagat attatttcca attcagatta 3181 agtactatct tagaatactg tttacttttt tggtttccat tttccaaagt ccgtgcaata 3241 tctcgaactt tcagcagaca cattacttgg tagacatctt tcggtctcaa atgcattgtc 3301 taaaaccttt cggatgtaga attattttaa aatttgattt gcattttgaa ttcagctaaa 3361 tttggcataa aaacattatt tcgttcagat cacaatttat tggtttacaa caattgtttt 3421 tggtttccgc tttttcttcc tgtaaaatgc aaaatcctga aaaaatatat cgtgaaaatg 3481 tttgggattc atttatcgac tgttaaactt taattttcca attttatgct aaaattgctc 3541 gaataaaact cgttttgaaa cggcgattga attggaactt cccgcgacaa cgttggaaac 3601 gagagacgca gacaaacggg gttaaaggca caccgaattt acaagacgag atatctcgcc 3661 gcgaaagtta ccagcactct cagaattgag gcccaattcc cactttcttt ttgtaaaatc 3721 aatttttttg cgtttttttt acgaatttgg cctaaaattt gtatatttac gcgaacaaaa 3781 aattaaattt gatttgttat cattgatttt ttgtcatatt cataacgaga acgtgtccaa 3841 aacttggaaa ataatttagg acgattttcc atcatcgtta ttaaaatttt aatatttctt 3901 gattttcttg ttggttatag taacgttact caatatttat atatttcttg ttattttttt 3961 aaatcatttt ttaaactata ctgaaGTTGT TATTTTGCAG GATAGCCGAT AACTGGATCC 4021 CCGGGATTGG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGCAGAAA 4081 AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGCAGA AAAAATGACT GCTCCAAAGA 4141 AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 4201 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 4261 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 4321 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 4381 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 4441 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 4501 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 4561 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 4621 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 4681 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 4741 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 4801 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 4861 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 4921 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 4981 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 5041 CTGGGATTAC ACATGGCATG GAcGAACTAT ACAAAagccc acaAGCTTTG GAATTCGCTA 5101 GCCGGCCATA CAAGTAATCC GGATGATCGA CGCCaACGTC GTTGAATTTT CAAATTTTAA 5161 ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC TCTTTGTGTT TTTTTTCTTG 5221 CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt ttttctcttt ccgtctccca 5281 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt 5341 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga 5401 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt 5461 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa 5521 attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCTTT CGTCTCGCGC GTTTCGGTGA 5581 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 5641 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 5701 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA 5761 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA 5821 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 5881 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 5941 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 6001 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 6061 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 6121 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 6181 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 6241 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 6301 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 6361 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 6421 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 6481 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 6541 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 6601 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 6661 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 6721 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 6781 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 6841 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 6901 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 6961 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 7021 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 7081 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 7141 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 7201 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 7261 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 7321 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 7381 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA 7441 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 7501 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 7561 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 7621 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 7681 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG 7741 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 7801 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT 7861 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT 7921 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG 7981 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 8041 Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA 8101 AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1656 LOCUS ADDGENE_1656 5610 bp DNA circular SYN 01/03/2005 DEFINITION L4455 complete plasmid sequence KEYWORDS . SOURCE L4455 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1656 at www.addgene.org FEATURES Location/Qualifiers source 1..5610 /organism="Addgene plasmid L4455" /mol_type="other DNA" misc_feature 18..50 /label="rpl28.-1.4k" misc_feature 1423..1454 /label="rpl28.5'" CDS 1498..2574 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYKSPQALEFASRPYK" misc_feature 1498..1524 /label="SV40.NLS" misc_feature 1540..1566 /label="SV40.NLS" misc_feature 1582..1608 /label="SV40.NLS" misc_feature 1624..1650 /label="SV40.NLS" gene 1666..2514 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1837..1887 /label="SynIVS.R" misc_feature 1909..1928 /label="gfp.S65C" misc_feature 2042..2092 /label="SynIVS.S" misc_feature 2257..2307 /label="SynIVS.T" misc_feature 2680..2709 /label="let858.3'" promoter 3390..3418 /label="Amp_promoter" gene 3460..4320 /label="Ampicillin" /gene="Ampicillin" CDS 3460..4320 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4475..5094 /label="pBR322_origin" misc_feature 5310..5336 /label="lacI.C" promoter 5403..5432 /label="lac_promoter" misc_feature 5467..5485 /label="M13r_primer" misc_feature 5499..5549 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAgtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc 61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa 121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca 181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catCTAGAAT 241 CCTTTGCTGT AGAAGAATTG CTCAATAAGA ACACGTGGAG CTCCTTTAAT TTTGATACGA 301 CCTTTCAACT CATCGACGTG ACTGGCGATC GAATGTCCAA GATGTTCTTC AAGATAGGAG 361 CGGAGATCAT TTTCGCAAGC ctgaaagata tttatgttga ttcaggattt aataatgttc 421 ccaacttacA AAAATGTCTC CGTCGACGTT TCTGATAGTT ACTAGCTCGA CATAATCAAA 481 ATCCAACGTT TTCTCATTCA GTAAATCCCT TTTTCGTTCC AAATAAAGAG GAAGAAGATG 541 GTCATGACGA CGTCGAATGT AGTATTGATG TGTCTTGGCT GCTTctgaaa tatttcgatg 601 cggtatgaat ttaatctgta tatacCTGTC GGTGGTGTCC ATCCGCTTGG TGTAGGATAC 661 TTTTCGTGCT CAGGAACATT CGGCACCACT TCTATTGGCA TCAATCTTTC CACATACGAC 721 CAATCGATAG GAGCTTCTTC GACCGTTGCA AGACTTCTGG ctgtaattgc tagtagaaaa 781 attatatgat aataaataac ttacACTTTT CAGGGAGAGC ATGTTTCCAT GGATTTTCCC 841 AAAGTTTCTC AGCTTTTTCG ACTGAAGTTG TTGAAAAAGA CAACCTGGAA ACTGCTCGAG 901 GGGAGAAAAT GCGGAGAGAA GAACCCAGCA Tcttaataaa attatataat tttgaataaa 961 atcaaattaa aaataagatt aattgaaaca caacaataga atgcagaaaa ttactcataa 1021 gaaataattt attttgaaag aagttgtgtc gatttacgat aaaaactaca cgcaaacgag 1081 gctccgcgtt tacaccgttt ggcaatggag cgcgcttgct attagatttt aggccagctc 1141 aacgaaagtt taatttattt ttcttctttt tttccatttt tttgagtaaa atatcgaatt 1201 ttttgtatta attctgtaaa ttttataatt tgcaactatt ttggcgtatt ctgcttgttt 1261 ttatcagcga atttttatgc atttcagtca aaagtttgag caggaaaaat gacatttagg 1321 actttgtaaa attgtattct taaatttgct tgaaaaccta cgtgtacaat tgattttatt 1381 gaatttttct tacgtttccc gttgtatctt attgtattta actttcatca taaaataatt 1441 tattTCCAGG TAAAATATCC GACGCTCTCG TGGATCCCCG GGATTGGCTC AGAAAAAATG 1501 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GCAGAAAAAA TGACTGCTCC AAAGAAGAAG 1561 CGTAAGGTAC CGGCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT ACCGGCAGAA 1621 AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA 1681 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 1741 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 1801 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 1861 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 1921 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 1981 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 2041 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 2101 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 2161 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 2221 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 2281 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 2341 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 2401 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 2461 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAc 2521 GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC GGCCATACAA GTAATCCGGA 2581 TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC 2641 CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC 2701 CAAATCTAAa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc 2761 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga 2821 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat 2881 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata 2941 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT 3001 CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 3061 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 3121 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 3181 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 3241 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 3301 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 3361 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 3421 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 3481 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 3541 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 3601 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 3661 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 3721 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 3781 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 3841 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 3901 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 3961 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 4021 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 4081 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 4141 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 4201 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 4261 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 4321 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 4381 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 4441 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 4501 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 4561 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 4621 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 4681 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 4741 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 4801 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 4861 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 4921 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 4981 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 5041 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 5101 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 5161 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 5221 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 5281 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 5341 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 5401 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 5461 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 5521 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 5581 ATGGATACGC TAACAACTTG GAAATGAAAT // 1657 LOCUS ADDGENE_1657 4693 bp DNA circular SYN 01/03/2005 DEFINITION L4458 complete plasmid sequence KEYWORDS . SOURCE L4458 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1657 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L4458" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1161..1192 /label="gfp.T203Y" misc_feature 1302..1352 /label="SynIVS.M" misc_feature 1429..1479 /label="SynIVS.L" promoter 2607..2635 /label="Amp_promoter" gene 2677..3537 /label="Ampicillin" /gene="Ampicillin" CDS 2677..3537 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3692..4311 /label="pBR322_origin" misc_feature 4527..4553 /label="lacI.C" promoter 4620..4649 /label="lac_promoter" misc_feature 4684..4702 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCTAC CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT Tgtaagttta aactttctca 1321 tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA 1381 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 1441 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 1501 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 1561 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 1621 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 1681 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 1741 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 1801 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 1861 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 1921 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 1981 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 2041 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 2101 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 2161 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 2221 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 2281 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 2341 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 2401 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 2461 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 2521 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 2581 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 2641 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 2701 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 2761 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 2821 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 2881 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 2941 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 3001 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 3061 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 3121 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 3181 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 3241 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 3301 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 3361 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 3421 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 3481 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 3541 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 3601 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 3661 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 3721 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 3781 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 3841 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 3901 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 3961 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 4021 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 4081 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 4141 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 4201 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 4261 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 4321 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 4381 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 4441 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 4501 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 4561 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 4621 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 4681 ACAGGAAACA GCT // 1658 LOCUS ADDGENE_1658 4693 bp DNA circular SYN 01/03/2005 DEFINITION L4506 complete plasmid sequence KEYWORDS . SOURCE L4506 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1658 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L4506" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFAYGLQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 785..816 /label="gfp.T203Y" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGCTTA TGGTCTTCAA 301 TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CTACCAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1659 LOCUS ADDGENE_1659 4693 bp DNA circular SYN 01/03/2005 DEFINITION L4507 complete plasmid sequence KEYWORDS . SOURCE L4507 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1659 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L4507" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGVQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 785..816 /label="gfp.T203Y" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGGTTA TGGTGTTCAA 301 TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CTACCAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1660 LOCUS ADDGENE_1660 3692 bp DNA circular SYN 01/03/2005 DEFINITION L453 complete plasmid sequence KEYWORDS . SOURCE L453 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1660 at www.addgene.org FEATURES Location/Qualifiers source 1..3692 /organism="Addgene plasmid L453" /mol_type="other DNA" misc_feature 13..31 /label="M13r_primer" promoter 48..67 /label="T3_promoter" CDS 622..1329 /label="Orf0" /translation="MKTITIKVGNDVEFDVPVRGEPPPKKEWIFNEKPVDDQKIRIES EDYKTRFVLRGATRKHAGLYTLTATNASGSDKHSVEVIVLGGDPLVLERPPPRWSSNS PYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLP LGFRFSALRHLDPKKLD" promoter complement(928..946) /label="T7_promoter" misc_feature 951..1111 /label="lacZ_a" misc_feature complement(954..970) /label="M13f20_primer" rep_origin 1129..1435 /label="f1_origin" promoter 1628..1656 /label="Amp_promoter" gene 1698..2558 /label="Ampicillin" /gene="Ampicillin" CDS 1698..2558 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2713..3332 /label="pBR322_origin" misc_feature 3548..3574 /label="lacI.C" promoter 3641..3670 /label="lac_promoter" ORIGIN 1 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac 61 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt 121 gatatcgAAT TCGGAAGATG GGTTCCATGC GCCAAAGTTA AGGATACGAA GGCTCATATT 181 GATGGTCTCA AGAAGGGACA AACATATCAG TTCCGTGTCA AGGCTGTCAA TAAGGAAGGA 241 GCTTCTGATG CATTGTCTAC TGATAAGGAC ACCAAAGCCA AGAATCCATA TGATGAGCCA 301 GGAAAAACCG GAACTCCGGA TGTTGTCGAC TGGGATGCTG ATCGTGTCTC ACTTGAATGG 361 GAACCACCAA AGTCTGACGG AGGAGCTCCA ATCACTCAAT ACGTCATTGA GAAGAAGGGC 421 AAACATGGAA GAGACTGGCA AGAATGCGGA AAGGTTTCTG GAGATCAAAC CAATGCTGAG 481 ATTCTTGGAC TCAAGGAGGG AGAAGAGTAC CAGTTCCGTG TGAAGGCTGT TAACAAGGCC 541 GGACCGGGAG AGGCTTCAGA CCCAAGCCGA AAGGTTGTTG CAAAGCCAAG AAACTTGAAG 601 CCATGGATTG ATCGTGAAGC AATGAAGACG ATCACTATCA AGGTAGGAAA CGATGTGGAA 661 TTCGATGTTC CAGTACGCGG AGAACCACCA CCGAAGAAGG AATGGATCTT CAATGAGAAA 721 CCAGTCGATG ATCAAAAGAT CAGGATTGAA AGCGAAGACT ACAAGACCCG ATTTGTGCTC 781 CGTGGAGCAA CTCGCAAGCA TGCTGGTTTG TACACTCTTA CTGCTACCAA CGCTTCTGGA 841 AGCGACAAAC ATTCCGTTGA GGTCATTGTG CTCGGggggg atccactagt tctagagcgg 901 ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc 961 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 1021 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 1081 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 1141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 1201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 1261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 1321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 1381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 1441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 1501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 1561 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 1621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 1681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 1741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 1801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 1861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 1921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 1981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 2041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 2101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 2161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 2221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 2281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 2341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 2401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 2461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 2521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 2581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 2641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 2701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 2761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 2821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 2881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 2941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 3421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 3481 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 3541 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 3601 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 3661 ctcgtatgtt gtgtggaatt gtgagcggat aa // 1661 LOCUS ADDGENE_1661 4693 bp DNA circular SYN 01/03/2005 DEFINITION L4632 complete plasmid sequence KEYWORDS . SOURCE L4632 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1661 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L4632" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 283..312 /label="gfp.GLA" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGGTTA TGGTCTTCAA 301 TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1662 LOCUS ADDGENE_1662 5731 bp DNA circular SYN 01/03/2005 DEFINITION L4640 complete plasmid sequence KEYWORDS . SOURCE L4640 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1662 at www.addgene.org FEATURES Location/Qualifiers source 1..5731 /organism="Addgene plasmid L4640" /mol_type="other DNA" misc_feature 57..81 /label="myo2.-1090" CDS 1032..2192 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWLASTVPVEKMSKGE ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIY ILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNY NSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNT PIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1145..1168 /label="myo2.5'" misc_feature 1242..1283 /label="SynIVS.A" misc_feature 1290..1307 /label="rf21.28" gene 1323..2183 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1494..1544 /label="SynIVS.R" misc_feature 1563..1592 /label="gfp.GLA" misc_feature 1699..1749 /label="SynIVS.S" misc_feature 1914..1964 /label="SynIVS.T" misc_feature 2065..2096 /label="gfp.T203Y" misc_feature 2206..2256 /label="SynIVS.M" misc_feature 2333..2383 /label="SynIVS.L" promoter 3511..3539 /label="Amp_promoter" CDS 3581..4441 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3581..4441 /label="Ampicillin" /gene="Ampicillin" rep_origin 4596..5215 /label="pBR322_origin" misc_feature 5431..5457 /label="lacI.C" promoter 5524..5553 /label="lac_promoter" misc_feature 5588..5606 /label="M13r_primer" misc_feature 5620..5670 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCAG CTTGCATGCC TGCAGGTCGA 61 GGCATTTGAA TTGGGGGTGG TGGACAGTAA CTGTCTGTAA TAATAATTAC TCCTGACCAG 121 GTTGCAATTC GAGTTTTGAT AAGCATAATT ATACCTTGTA CATTGTGGGT TTTGTGCTGT 181 GGACGTTTTA TTGTGACATC CCCATAAGCT ACAAGAACAA AAATGAAATg TAAAAGTATT 241 GAAAACGTCG TACATTTTAT ATCTGAGTAG TATCCTTTGC TTTAAATGTC CATAAAAcTA 301 ATTTTATAAT CAATAAAACA ACGTTTGTAA ATCAACTGAG TTTACAAGTA GAGACATTGA 361 GGGATACTTT CACTATGCTA AAGTGATAAT CGACCAAATA ATAACTtCAC TTTGGTATTT 421 ATTCCTGTCT TATAAaTGTT ATGTATGAAT TAAATTCATA TGCATATGGC TCACTCTGAC 481 AAtttttAAT AATCTTCCAG ATCAATATTG ACTACCGATG CGGGTGGTCT TTTGCTTTGA 541 ATTCTGCTGA ACTTTACACC CCGAACAGCA ATGTGTGCTT CAGCcTAAAA AAAAGTAAGT 601 GTGTTAATCA GTgCCCCCGA TTCTTCATTT TTTGCCCCTC TCTCCCGTTT CGTCGGCAAA 661 AGAAGAGAAA ATAAAGATAA GTCTCAAGAT AGGTTGGTAA TCGCTAAAGT GGTTGTGTGG 721 ATAAGAGTAG CAAAATGGCA GGAAGAGCAC TTTGCGCGCA CACACTGTAC TCATTGTTCT 781 GGATAAAATT CTCTCGTTGT TTGCCGTCGG ATGTCTGCCT CTCTGCATTG AGCCGGCTTC 841 TTCACTATCT TTAGTTAACC TAAAATGCCG TTTCTTTTCT CGTATCCCcA CTATcCCGTT 901 GAGGTTCTCT GCTCTCTTCG CTCCCTACCG CCAGCGAGCA ACTATCCGTG GGGGCGCCTT 961 GCTCGGAAGA TGGGGGGGAA GAAAGAAGAT TTTTGCTATT TGCACTTGAG AAAGAGACTT 1021 TTCCTGCGTC GATGGTTAGA GAACAGTGTG CAGACACTTT TCAGCTACCT AGAatTACAa 1081 tTGGATATCC CCGCCTCCCA ATCCACCCAC CCAGGGAAAA AGAAGGGCTC GCCGAAAATC 1141 AAAGTTATCT CCAGGCTCGC GCATCCCACC GAGCGGTTGA CTTCTCTCCA CCACTTTTCA 1201 TTTTAACCCT CGGGGTACGG GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT 1261 AACATAACAT AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCGGTAGAAA 1321 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 1381 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 1441 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 1501 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 1561 CTTTCGGTTA TGGTCTTCAA TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT 1621 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 1681 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 1741 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 1801 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 1861 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 1921 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 1981 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 2041 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CTACCAATCT GCCCTTTCGA 2101 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 2161 TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt 2221 ctcatactaa ctaacgcgct ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT 2281 ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt 2341 aaactgagtt ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC 2401 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 2461 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 2521 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 2581 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 2641 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 2701 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 2761 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 2821 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 2881 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 2941 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 3001 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 3061 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 3121 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 3181 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 3241 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 3301 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 3361 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 3421 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 3481 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 3541 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 3601 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 3661 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 3721 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 3781 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 3841 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 3901 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 3961 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 4021 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 4081 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 4141 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 4201 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 4261 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 4321 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 4381 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 4441 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 4501 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 4561 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 4621 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 4681 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 4741 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 4801 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 4861 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 4921 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4981 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 5041 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 5101 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 5161 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 5221 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 5281 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 5341 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 5401 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 5461 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 5521 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 5581 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta 5641 ctaactaact attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC 5701 GATGGATACG CTAACAACTT GGAAATGAAA T // 1663 LOCUS ADDGENE_1663 6800 bp DNA circular SYN 01/03/2005 DEFINITION L4643 complete plasmid sequence KEYWORDS . SOURCE L4643 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1663 at www.addgene.org FEATURES Location/Qualifiers source 1..6800 /organism="Addgene plasmid L4643" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" CDS 2484..3395 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2484..2510 /label="SV40.NLS" gene 2526..3386 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2697..2747 /label="SynIVS.R" misc_feature 2766..2795 /label="gfp.GLA" misc_feature 2902..2952 /label="SynIVS.S" misc_feature 3117..3167 /label="SynIVS.T" misc_feature 3268..3299 /label="gfp.T203Y" misc_feature 3409..3459 /label="SynIVS.M" misc_feature 3536..3586 /label="SynIVS.L" promoter 4714..4742 /label="Amp_promoter" gene 4784..5644 /label="Ampicillin" /gene="Ampicillin" CDS 4784..5644 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5799..6418 /label="pBR322_origin" misc_feature 6634..6660 /label="lacI.C" promoter 6727..6756 /label="lac_promoter" misc_feature 6791..6809 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG 2521 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 2581 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 2641 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 2701 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 2761 CTACTTTCGG TTATGGTCTT CAATGCTTCG CCAGATACCC AGATCATATG AAACAGCATG 2821 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 2881 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 2941 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 3001 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 3061 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 3121 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 3181 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 3241 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCTACCAA TCTGCCCTTT 3301 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 3361 GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac 3421 tttctcatac taactaacgc gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC 3481 GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag 3541 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT 3601 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 3661 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 3721 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 3781 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 3841 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 3901 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 3961 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 4021 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 4081 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 4141 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 4201 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 4261 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT 4321 AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 4381 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 4441 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA 4501 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 4561 AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 4621 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 4681 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 4741 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 4801 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4861 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 4921 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 4981 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 5041 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 5101 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 5161 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 5221 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 5281 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 5341 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 5401 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5461 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 5521 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 5581 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 5641 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 5701 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 5761 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 5821 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 5881 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 5941 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 6001 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 6061 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 6121 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 6181 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 6241 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 6301 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 6361 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 6421 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 6481 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 6541 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 6601 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 6661 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 6721 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 6781 ATTTCACACA GGAAACAGCT // 1664 LOCUS ADDGENE_1664 10324 bp DNA circular SYN 01/03/2005 DEFINITION L4660 complete plasmid sequence KEYWORDS . SOURCE L4660 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1664 at www.addgene.org FEATURES Location/Qualifiers source 1..10324 /organism="Addgene plasmid L4660" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" CDS 2484..4079 /label="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 2484..2510 /label="SV40.NLS" misc_feature 2697..2747 /label="SynIVS.R" misc_feature 2772..2796 /label="gfp.Y66W" misc_feature 2902..2952 /label="SynIVS.S" misc_feature 3056..3080 /label="gfp.N146I" misc_feature 3075..3098 /label="gfp.M153T" misc_feature 3095..3116 /label="gfp.V163A" misc_feature 3117..3167 /label="SynIVS.T" gene 3483..7036 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 3617..3667 /label="SynIVS.B" CDS 4118..7039 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 4295..4345 /label="SynIVS.D" misc_feature 4736..4786 /label="SynIVS.E" misc_feature 5043..5093 /label="SynIVS.F" misc_feature 5324..5374 /label="SynIVS.G" misc_feature 5611..5661 /label="SynIVS.H" misc_feature 5977..6027 /label="SynIVS.I" misc_feature 6288..6338 /label="SynIVS.J" misc_feature 6694..6744 /label="SynIVS.K" misc_feature 7060..7110 /label="SynIVS.L" promoter 8238..8266 /label="Amp_promoter" CDS 8308..9168 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8308..9168 /label="Ampicillin" /gene="Ampicillin" rep_origin 9323..9942 /label="pBR322_origin" misc_feature 10158..10184 /label="lacI.C" promoter 10251..10280 /label="lac_promoter" misc_feature 10315..10333 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG 2521 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 2581 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 2641 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 2701 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 2761 CTACTTTCTC ATGGGGCGTT CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG 2821 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 2881 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 2941 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 3001 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 3061 ATATTTCACA CAACGTATAC ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa 3121 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 3181 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 3241 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 3301 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 3361 GGATTACACA TGGCATGGAT GAACTATACA AATgcCCGGT GGGTGAAGAC CAGAAACAGC 3421 ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC GAGATCGATC 3481 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 3541 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 3601 CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt tctgacataa 3661 ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG 3721 AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC 3781 AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC 3841 CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG 3901 AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC TCGGCGTTTC 3961 ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt aagttgataC 4021 TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG 4081 CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG 4141 CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT 4201 GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA 4261 TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga tcttactaac 4321 taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 4381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 4441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 4501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 4561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 4621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 4681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 4741 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 4801 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 4861 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 4921 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 4981 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 5041 TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG 5101 CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC 5161 GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA 5221 CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG 5281 GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt taaacagaac 5341 tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG GcAACAGTCT 5401 TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC AGGGCGGCTT 5461 CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA ACCCGTGGTC 5521 GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA TGAACGGTCT 5581 GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct actaactaac 5641 gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT 5701 CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA 5761 CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC 5821 TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA 5881 GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC 5941 AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa gatcctacta 6001 actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC 6061 CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG 6121 TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA 6181 TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA 6241 CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta agtttaaaca 6301 aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG CGGCGGGCCA 6361 TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT 6421 GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC 6481 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA 6541 TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT 6601 AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT 6661 TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat agtactaact 6721 aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG 6781 CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA 6841 CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC 6901 GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC 6961 CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT 7021 TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta 7081 ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG 7141 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 7201 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 7261 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 7321 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 7381 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 7441 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 7501 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 7561 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 7621 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 7681 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 7741 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 7801 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 7861 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 7921 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 7981 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 8041 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 8101 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 8161 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 8221 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 8281 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 8341 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 8401 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 8461 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 8521 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 8581 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 8641 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 8701 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 8761 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 8821 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 8881 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 8941 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 9001 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 9061 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 9121 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 9181 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 9241 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 9301 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 9361 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 9421 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 9481 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 9541 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 9601 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 9661 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 9721 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 9781 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 9841 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 9901 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 9961 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 10021 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 10081 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 10141 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 10201 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 10261 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 10321 AGCT // 1665 LOCUS ADDGENE_1665 6800 bp DNA circular SYN 01/03/2005 DEFINITION L4663 complete plasmid sequence KEYWORDS . SOURCE L4663 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1665 at www.addgene.org FEATURES Location/Qualifiers source 1..6800 /organism="Addgene plasmid L4663" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2484..2510 /label="SV40.NLS" CDS 2484..3395 /label="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCF SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2697..2747 /label="SynIVS.R" misc_feature 2772..2796 /label="gfp.Y66W" misc_feature 2902..2952 /label="SynIVS.S" misc_feature 3056..3080 /label="gfp.N146I" misc_feature 3075..3098 /label="gfp.M153T" misc_feature 3095..3116 /label="gfp.V163A" misc_feature 3117..3167 /label="SynIVS.T" misc_feature 3409..3459 /label="SynIVS.M" misc_feature 3536..3586 /label="SynIVS.L" promoter 4714..4742 /label="Amp_promoter" gene 4784..5644 /label="Ampicillin" /gene="Ampicillin" CDS 4784..5644 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5799..6418 /label="pBR322_origin" misc_feature 6634..6660 /label="lacI.C" promoter 6727..6756 /label="lac_promoter" misc_feature 6791..6809 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG 2521 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 2581 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 2641 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 2701 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 2761 CTACTTTCTC ATGGGGCGTT CAATGCTTCT CGAGATACCC AGATCATATG AAACAGCATG 2821 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 2881 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 2941 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 3001 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 3061 ATATTTCACA CAACGTATAC ATCACTGCCG ACAAACAAAA GAATGGGATC AAAGCGgtaa 3121 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 3181 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 3241 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 3301 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 3361 GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC GTAGAATTgt aagtttaaac 3421 tttctcatac taactaacgc gctctattta aattttcagA ATTCCAACTG AGCGCCGGTC 3481 GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag 3541 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT 3601 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 3661 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 3721 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 3781 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 3841 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 3901 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 3961 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 4021 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 4081 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 4141 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 4201 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 4261 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT 4321 AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 4381 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 4441 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA 4501 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 4561 AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 4621 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 4681 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 4741 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 4801 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4861 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 4921 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 4981 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 5041 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 5101 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 5161 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 5221 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 5281 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 5341 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 5401 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5461 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 5521 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 5581 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 5641 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 5701 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 5761 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 5821 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 5881 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 5941 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 6001 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 6061 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 6121 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 6181 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 6241 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 6301 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 6361 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 6421 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 6481 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 6541 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 6601 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 6661 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 6721 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 6781 ATTTCACACA GGAAACAGCT // 1666 LOCUS ADDGENE_1666 7172 bp DNA circular SYN 01/03/2005 DEFINITION L4664 complete plasmid sequence KEYWORDS . SOURCE L4664 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1666 at www.addgene.org FEATURES Location/Qualifiers source 1..7172 /organism="Addgene plasmid L4664" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2503..3633 /label="Orf0" /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTPDNATVWVRKHKDCP PPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFSWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITAD KQKNGIKAVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2503..2533 /label="pat3.MbLS" misc_feature 2935..2985 /label="SynIVS.R" misc_feature 3010..3034 /label="gfp.Y66W" misc_feature 3140..3190 /label="SynIVS.S" misc_feature 3294..3318 /label="gfp.N146I" misc_feature 3313..3336 /label="gfp.M153T" misc_feature 3333..3354 /label="gfp.V163A" misc_feature 3355..3405 /label="SynIVS.T" misc_feature 3647..3697 /label="SynIVS.M" misc_feature 3774..3824 /label="SynIVS.L" promoter 4952..4980 /label="Amp_promoter" gene 5022..5882 /label="Ampicillin" /gene="Ampicillin" CDS 5022..5882 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6037..6656 /label="pBR322_origin" misc_feature 6872..6898 /label="lacI.C" promoter 6965..6994 /label="lac_promoter" misc_feature 7029..7047 /label="M13r_primer" misc_feature 7061..7111 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGg gtaccgccgg caatgccacc ttcaacatca 2521 ttgctgctcc tcgcagcact tcttccattc gctttaccag caagcgattg gaagactgga 2581 gaagtcactc cggataatgc aacagtctgg gtcagaaaac ataaagattg tcctccacct 2641 gtccctgtgc tcgcaattgt gctcggagtc attgcgggta tcgtaatcct cggaattctt 2701 ctcttgttgc tctggaaatt gctcacagta cttcatgata gatccgaggt aCCGGTAGAA 2761 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 2821 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 2881 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 2941 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 3001 ACTTTCTCAT GGGGCGTTCA ATGCTTCTCG AGATACCCAG ATCATATGAA ACAGCATGAC 3061 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 3121 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 3181 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 3241 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 3301 ATTTCACACA ACGTATACAT CACTGCCGAC AAACAAAAGA ATGGGATCAA AGCGgtaagt 3361 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 3421 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 3481 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 3541 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 3601 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTgtaa gtttaaactt 3661 tctcatacta actaacgcgc tctatttaaa ttttcagAAT TCCAACTGAG CGCCGGTCGC 3721 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 3781 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 3841 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 3901 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 3961 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 4021 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 4081 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 4141 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 4201 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 4261 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 4321 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 4381 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 4441 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 4501 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 4561 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 4621 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 4681 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 4741 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 4801 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 4861 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 4921 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 4981 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 5041 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 5101 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 5161 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 5221 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 5281 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 5341 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 5401 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 5461 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 5521 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 5581 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 5641 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 5701 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 5761 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 5821 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 5881 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 5941 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 6001 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 6061 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 6121 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 6181 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 6241 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 6301 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 6361 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 6421 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 6481 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 6541 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 6601 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 6661 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 6721 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 6781 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 6841 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 6901 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 6961 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 7021 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 7081 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 7141 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1667 LOCUS ADDGENE_1667 6893 bp DNA circular SYN 01/03/2005 DEFINITION L4665 complete plasmid sequence KEYWORDS . SOURCE L4665 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1667 at www.addgene.org FEATURES Location/Qualifiers source 1..6893 /organism="Addgene plasmid L4665" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2499..2516 /label="rf21.28" misc_feature 2532..2605 /label="ATG-MitLS" CDS 2532..3488 /label="Orf2" /translation="MALLQSRLLLSAPRRAAATARASSVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFSWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITAD KQKNGIKAVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2790..2840 /label="SynIVS.R" misc_feature 2865..2889 /label="gfp.Y66W" misc_feature 2995..3045 /label="SynIVS.S" misc_feature 3149..3173 /label="gfp.N146I" misc_feature 3168..3191 /label="gfp.M153T" misc_feature 3188..3209 /label="gfp.V163A" misc_feature 3210..3260 /label="SynIVS.T" misc_feature 3502..3552 /label="SynIVS.M" misc_feature 3629..3679 /label="SynIVS.L" promoter 4807..4835 /label="Amp_promoter" CDS 4877..5737 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4877..5737 /label="Ampicillin" /gene="Ampicillin" rep_origin 5892..6511 /label="pBR322_origin" misc_feature 6727..6753 /label="lacI.C" promoter 6820..6849 /label="lac_promoter" misc_feature 6884..6902 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG gatccccggg attggccaaa ggacccaaag gtatgtttcg 2461 aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg tcgacgGTAC 2521 ctcgagaaaa aatggcactc ctgcaatcac gtctcctgct atccgcacct cgtcgtgccg 2581 ctgcaactgc aagagcttca tccGTACCGG TAGAAAAAAT GAGTAAAGGA GAAGAACTTT 2641 TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT 2701 CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT 2761 GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa catatatata ctaactaacc 2821 ctgattattt aaattttcag CCAACACTTG TCACTACTTT CTCATGGGGC GTTCAATGCT 2881 TCTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG 2941 GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACgtaagt 3001 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagGTGCT GAAGTCAAGT 3061 TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG 3121 GAAACATTCT TGGACACAAA TTGGAATACA ACTATATTTC ACACAACGTA TACATCACTG 3181 CCGACAAACA AAAGAATGGG ATCAAAGCGg taagtttaaa catgatttta ctaactaact 3241 aatctgattt aaattttcag AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC 3301 AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG 3361 ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC 3421 ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT 3481 ACAAATAGCA TTCGTAGAAT Tgtaagttta aactttctca tactaactaa cgcgctctat 3541 ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC 3601 AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac tgagttctac taactaacga 3661 gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC 3721 TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA 3781 AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA 3841 TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC 3901 TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA 3961 TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC 4021 CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC 4081 CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT 4141 CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA 4201 TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC 4261 ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA 4321 GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA 4381 TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC 4441 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA 4501 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT 4561 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 4621 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT 4681 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 4741 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 4801 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 4861 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 4921 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 4981 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 5041 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 5101 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 5161 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 5221 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 5281 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 5341 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 5401 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 5461 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 5521 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 5581 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 5641 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 5701 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 5761 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 5821 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 5881 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 5941 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 6001 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 6061 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 6121 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 6181 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 6241 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA 6301 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 6361 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 6421 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 6481 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 6541 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 6601 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 6661 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 6721 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 6781 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 6841 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1668 LOCUS ADDGENE_1668 7172 bp DNA circular SYN 01/03/2005 DEFINITION L4666 complete plasmid sequence KEYWORDS . SOURCE L4666 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1668 at www.addgene.org FEATURES Location/Qualifiers source 1..7172 /organism="Addgene plasmid L4666" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" CDS 2503..3633 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTPDNATVWVRKHKDCP PPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2503..2533 /label="pat3.MbLS" gene 2764..3624 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2935..2985 /label="SynIVS.R" misc_feature 3004..3033 /label="gfp.GLA" misc_feature 3140..3190 /label="SynIVS.S" misc_feature 3355..3405 /label="SynIVS.T" misc_feature 3506..3537 /label="gfp.T203Y" misc_feature 3647..3697 /label="SynIVS.M" misc_feature 3774..3824 /label="SynIVS.L" promoter 4952..4980 /label="Amp_promoter" gene 5022..5882 /label="Ampicillin" /gene="Ampicillin" CDS 5022..5882 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6037..6656 /label="pBR322_origin" misc_feature 6872..6898 /label="lacI.C" promoter 6965..6994 /label="lac_promoter" misc_feature 7029..7047 /label="M13r_primer" misc_feature 7061..7111 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGg gtaccgccgg caatgccacc ttcaacatca 2521 ttgctgctcc tcgcagcact tcttccattc gctttaccag caagcgattg gaagactgga 2581 gaagtcactc cggataatgc aacagtctgg gtcagaaaac ataaagattg tcctccacct 2641 gtccctgtgc tcgcaattgt gctcggagtc attgcgggta tcgtaatcct cggaattctt 2701 ctcttgttgc tctggaaatt gctcacagta cttcatgata gatccgaggt aCCGGTAGAA 2761 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 2821 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 2881 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 2941 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 3001 ACTTTCGGTT ATGGTCTTCA ATGCTTCGCC AGATACCCAG ATCATATGAA ACAGCATGAC 3061 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 3121 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 3181 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 3241 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 3301 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 3361 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 3421 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 3481 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCTACCAATC TGCCCTTTCG 3541 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 3601 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTgtaa gtttaaactt 3661 tctcatacta actaacgcgc tctatttaaa ttttcagAAT TCCAACTGAG CGCCGGTCGC 3721 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 3781 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 3841 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 3901 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 3961 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 4021 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 4081 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 4141 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 4201 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 4261 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 4321 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 4381 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 4441 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 4501 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 4561 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 4621 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 4681 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 4741 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 4801 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 4861 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 4921 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 4981 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 5041 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 5101 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 5161 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 5221 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 5281 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 5341 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 5401 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 5461 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 5521 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 5581 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 5641 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 5701 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 5761 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 5821 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 5881 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 5941 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 6001 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 6061 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 6121 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 6181 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 6241 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 6301 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 6361 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 6421 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 6481 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 6541 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 6601 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 6661 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 6721 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 6781 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 6841 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 6901 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 6961 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 7021 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 7081 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 7141 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1669 LOCUS ADDGENE_1669 6893 bp DNA circular SYN 01/03/2005 DEFINITION L4667 complete plasmid sequence KEYWORDS . SOURCE L4667 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1669 at www.addgene.org FEATURES Location/Qualifiers source 1..6893 /organism="Addgene plasmid L4667" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2499..2516 /label="rf21.28" CDS 2532..3488 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MALLQSRLLLSAPRRAAATARASSVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 2532..2605 /label="ATG-MitLS" gene 2619..3479 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2790..2840 /label="SynIVS.R" misc_feature 2859..2888 /label="gfp.GLA" misc_feature 2995..3045 /label="SynIVS.S" misc_feature 3210..3260 /label="SynIVS.T" misc_feature 3361..3392 /label="gfp.T203Y" misc_feature 3502..3552 /label="SynIVS.M" misc_feature 3629..3679 /label="SynIVS.L" promoter 4807..4835 /label="Amp_promoter" CDS 4877..5737 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4877..5737 /label="Ampicillin" /gene="Ampicillin" rep_origin 5892..6511 /label="pBR322_origin" misc_feature 6727..6753 /label="lacI.C" promoter 6820..6849 /label="lac_promoter" misc_feature 6884..6902 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG gatccccggg attggccaaa ggacccaaag gtatgtttcg 2461 aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg tcgacgGTAC 2521 ctcgagaaaa aatggcactc ctgcaatcac gtctcctgct atccgcacct cgtcgtgccg 2581 ctgcaactgc aagagcttca tccGTACCGG TAGAAAAAAT GAGTAAAGGA GAAGAACTTT 2641 TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT 2701 CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT 2761 GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa catatatata ctaactaacc 2821 ctgattattt aaattttcag CCAACACTTG TCACTACTTT CGGTTATGGT CTTCAATGCT 2881 TCGCCAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG 2941 GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACgtaagt 3001 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagGTGCT GAAGTCAAGT 3061 TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG 3121 GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG 3181 CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa catgatttta ctaactaact 3241 aatctgattt aaattttcag AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC 3301 AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG 3361 ACAACCATTA CCTGTCCTAC CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC 3421 ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT 3481 ACAAATAGCA TTCGTAGAAT Tgtaagttta aactttctca tactaactaa cgcgctctat 3541 ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC 3601 AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac tgagttctac taactaacga 3661 gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC 3721 TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA 3781 AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA 3841 TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC 3901 TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA 3961 TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC 4021 CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC 4081 CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT 4141 CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA 4201 TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC 4261 ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA 4321 GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA 4381 TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC 4441 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA 4501 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT 4561 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 4621 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT 4681 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 4741 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 4801 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 4861 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 4921 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 4981 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 5041 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 5101 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 5161 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 5221 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 5281 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 5341 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 5401 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 5461 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 5521 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 5581 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 5641 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 5701 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 5761 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 5821 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 5881 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 5941 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 6001 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 6061 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 6121 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 6181 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 6241 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA 6301 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 6361 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 6421 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 6481 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 6541 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 6601 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 6661 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 6721 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 6781 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 6841 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1670 LOCUS ADDGENE_1670 10324 bp DNA circular SYN 01/03/2005 DEFINITION L4671 complete plasmid sequence KEYWORDS . SOURCE L4671 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1670 at www.addgene.org FEATURES Location/Qualifiers source 1..10324 /organism="Addgene plasmid L4671" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2484..2510 /label="SV40.NLS" CDS 2484..4079 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCF ARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 2526..3386 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2697..2747 /label="SynIVS.R" misc_feature 2766..2795 /label="gfp.GLA" misc_feature 2902..2952 /label="SynIVS.S" misc_feature 3117..3167 /label="SynIVS.T" misc_feature 3268..3299 /label="gfp.T203Y" gene 3483..7036 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 3617..3667 /label="SynIVS.B" CDS 4118..7039 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 4295..4345 /label="SynIVS.D" misc_feature 4736..4786 /label="SynIVS.E" misc_feature 5043..5093 /label="SynIVS.F" misc_feature 5324..5374 /label="SynIVS.G" misc_feature 5611..5661 /label="SynIVS.H" misc_feature 5977..6027 /label="SynIVS.I" misc_feature 6288..6338 /label="SynIVS.J" misc_feature 6694..6744 /label="SynIVS.K" misc_feature 7060..7110 /label="SynIVS.L" promoter 8238..8266 /label="Amp_promoter" CDS 8308..9168 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8308..9168 /label="Ampicillin" /gene="Ampicillin" rep_origin 9323..9942 /label="pBR322_origin" misc_feature 10158..10184 /label="lacI.C" promoter 10251..10280 /label="lac_promoter" misc_feature 10315..10333 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTAG 2521 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 2581 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 2641 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 2701 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 2761 CTACTTTCGG TTATGGTCTT CAATGCTTCG CCAGATACCC AGATCATATG AAACAGCATG 2821 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 2881 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 2941 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 3001 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 3061 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 3121 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 3181 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 3241 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCTACCAA TCTGCCCTTT 3301 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 3361 GGATTACACA TGGCATGGAT GAACTATACA AATgcCCGGT GGGTGAAGAC CAGAAACAGC 3421 ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC GAGATCGATC 3481 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 3541 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 3601 CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt tctgacataa 3661 ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG 3721 AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC 3781 AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC 3841 CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG 3901 AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC TCGGCGTTTC 3961 ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt aagttgataC 4021 TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG 4081 CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG 4141 CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT 4201 GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA 4261 TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga tcttactaac 4321 taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 4381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 4441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 4501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 4561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 4621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 4681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 4741 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 4801 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 4861 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 4921 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 4981 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 5041 TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG 5101 CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC 5161 GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA 5221 CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG 5281 GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt taaacagaac 5341 tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG GcAACAGTCT 5401 TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC AGGGCGGCTT 5461 CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA ACCCGTGGTC 5521 GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA TGAACGGTCT 5581 GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct actaactaac 5641 gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT 5701 CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA 5761 CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC 5821 TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA 5881 GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC 5941 AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa gatcctacta 6001 actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC 6061 CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG 6121 TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA 6181 TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA 6241 CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta agtttaaaca 6301 aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG CGGCGGGCCA 6361 TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT 6421 GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC 6481 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA 6541 TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT 6601 AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT 6661 TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat agtactaact 6721 aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG 6781 CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA 6841 CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC 6901 GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC 6961 CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT 7021 TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta 7081 ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG 7141 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 7201 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 7261 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 7321 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 7381 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 7441 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 7501 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 7561 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 7621 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 7681 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 7741 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 7801 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 7861 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 7921 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 7981 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 8041 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 8101 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 8161 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 8221 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 8281 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 8341 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 8401 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 8461 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 8521 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 8581 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 8641 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 8701 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 8761 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 8821 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 8881 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 8941 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 9001 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 9061 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 9121 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 9181 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 9241 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 9301 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 9361 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 9421 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 9481 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 9541 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 9601 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 9661 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 9721 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 9781 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 9841 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 9901 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 9961 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 10021 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 10081 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 10141 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 10201 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 10261 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 10321 AGCT // 1671 LOCUS ADDGENE_1671 10279 bp DNA circular SYN 01/03/2005 DEFINITION L4682 complete plasmid sequence KEYWORDS . SOURCE L4682 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1671 at www.addgene.org FEATURES Location/Qualifiers source 1..10279 /organism="Addgene plasmid L4682" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" CDS 2481..4034 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQ LNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFP APEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGC YSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFR TVVCRLNLT" misc_feature 2652..2702 /label="SynIVS.R" misc_feature 2727..2751 /label="gfp.Y66W" misc_feature 2857..2907 /label="SynIVS.S" misc_feature 3011..3035 /label="gfp.N146I" misc_feature 3030..3053 /label="gfp.M153T" misc_feature 3050..3071 /label="gfp.V163A" misc_feature 3072..3122 /label="SynIVS.T" gene 3438..6991 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 3572..3622 /label="SynIVS.B" CDS 4073..6994 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 4250..4300 /label="SynIVS.D" misc_feature 4691..4741 /label="SynIVS.E" misc_feature 4998..5048 /label="SynIVS.F" misc_feature 5279..5329 /label="SynIVS.G" misc_feature 5566..5616 /label="SynIVS.H" misc_feature 5932..5982 /label="SynIVS.I" misc_feature 6243..6293 /label="SynIVS.J" misc_feature 6649..6699 /label="SynIVS.K" misc_feature 7015..7065 /label="SynIVS.L" promoter 8193..8221 /label="Amp_promoter" CDS 8263..9123 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8263..9123 /label="Ampicillin" /gene="Ampicillin" rep_origin 9278..9897 /label="pBR322_origin" misc_feature 10113..10139 /label="lacI.C" promoter 10206..10235 /label="lac_promoter" misc_feature 10270..10288 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 2521 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 2581 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 2641 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 2701 agCCAACACT TGTCACTACT TTCTCATGGG GCGTTCAATG CTTCTCGAGA TACCCAGATC 2761 ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 2821 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 2881 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 2941 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 3001 AATTGGAATA CAACTATATT TCACACAACG TATACATCAC TGCCGACAAA CAAAAGAATG 3061 GGATCAAAGC Ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 3121 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 3181 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA 3241 CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 3301 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATgc CCGGTGGGTG 3361 AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC AACGCGCTGT 3421 ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 3481 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 3541 GCACCGATCG CCCTTCCCAA CAGTTGCGaA Ggtaagttta aacagatcca tactaactaa 3601 cttgttctga cataattttc agCtTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC 3661 CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG 3721 TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT CTACACCAAC GTGACCTATC 3781 CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA 3841 CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG 3901 TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT CGGTTACGGC CAGgtaagtt 3961 taattaagtt gataCTAACT AACAAGATCT GATTAATTTT CagGACAGTC GTTTGCCGTC 4021 TGAATTTGAC CTGAGCGCAT TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT 4081 GCGCTGGAGT GACGGCAGTT ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT 4141 CCGTGACGTC TCGTTGCTGC ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC 4201 TCGCTTTAAT GATGATTTCt ccCGCGCTGT ACTGGAGGCT GAAGTcCAGg taagtttaaa 4261 caggatctta ctaactaaca tgctaacact gaattttcag ATGTGCGGCG AGTTGCGTGA 4321 CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC 4381 GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG 4441 TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT 4501 GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT 4561 CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG 4621 AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT 4681 GGTGCAGGAT gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca 4741 gATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA 4801 TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT 4861 TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC 4921 GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT 4981 CTGGTCGCTG GGGAATGgta agtttaaaca gttgaatact aactaacgga gatctttgaa 5041 attttcagAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT 5101 CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT 5161 TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG 5221 GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATtC TTTGCGAGgt 5281 aagtttaaac agaactctac taactaacac attagatcct aattttcagT ACGCtCACGC 5341 GATGGGcAAC AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG 5401 TTTACAGGGC GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA 5461 CGGCAACCCG TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT 5521 CTGTATGAAC GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat 5581 aacctactaa ctaacgtaga taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA 5641 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 5701 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 5761 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 5821 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 5881 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCaTGG CAGCAGTGGA Ggtaagttta 5941 aacaagatcc tactaactaa ctctacattg atgaattttc agACTGGCGG AAAACCTCAG 6001 TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG ACCACCAGCG AAATGGATTT 6061 TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC CAGTCAGGCT TTCTTTCACA 6121 GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG CGCGATCAGT TCACCCGTGC 6181 ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC ATTGACCCTA ACGCCTGGGT 6241 CGgtaagttt aaacaaagtt gtactaacta acgaagatct tgataatttt cagAACGCTG 6301 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 6361 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 6421 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 6481 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 6541 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 6601 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 6661 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 6721 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 6781 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 6841 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 6901 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 6961 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt 7021 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG 7081 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 7141 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 7201 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 7261 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 7321 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT 7381 CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA 7441 ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA 7501 AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT 7561 GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT 7621 TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT 7681 TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG 7741 GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA 7801 GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 7861 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 7921 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 7981 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 8041 GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT 8101 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 8161 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 8221 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 8281 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 8341 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 8401 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 8461 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 8521 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 8581 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 8641 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 8701 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 8761 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 8821 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 8881 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 8941 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 9001 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 9061 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 9121 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 9181 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 9241 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 9301 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 9361 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 9421 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 9481 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 9541 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 9601 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 9661 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 9721 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 9781 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 9841 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 9901 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 9961 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC 10021 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA 10081 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC 10141 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC 10201 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 10261 TTTCACACAG GAAACAGCT // 1672 LOCUS ADDGENE_1672 10279 bp DNA circular SYN 01/03/2005 DEFINITION L4683 complete plasmid sequence KEYWORDS . SOURCE L4683 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1672 at www.addgene.org FEATURES Location/Qualifiers source 1..10279 /organism="Addgene plasmid L4683" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" CDS 2481..4034 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQ LNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFP APEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGC YSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFR TVVCRLNLT" gene 2481..3341 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2652..2702 /label="SynIVS.R" misc_feature 2721..2750 /label="gfp.GLA" misc_feature 2857..2907 /label="SynIVS.S" misc_feature 3072..3122 /label="SynIVS.T" misc_feature 3223..3254 /label="gfp.T203Y" gene 3438..6991 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 3572..3622 /label="SynIVS.B" CDS 4073..6994 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 4250..4300 /label="SynIVS.D" misc_feature 4691..4741 /label="SynIVS.E" misc_feature 4998..5048 /label="SynIVS.F" misc_feature 5279..5329 /label="SynIVS.G" misc_feature 5566..5616 /label="SynIVS.H" misc_feature 5932..5982 /label="SynIVS.I" misc_feature 6243..6293 /label="SynIVS.J" misc_feature 6649..6699 /label="SynIVS.K" misc_feature 7015..7065 /label="SynIVS.L" promoter 8193..8221 /label="Amp_promoter" gene 8263..9123 /label="Ampicillin" /gene="Ampicillin" CDS 8263..9123 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 9278..9897 /label="pBR322_origin" misc_feature 10113..10139 /label="lacI.C" promoter 10206..10235 /label="lac_promoter" misc_feature 10270..10288 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 2521 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 2581 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 2641 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 2701 agCCAACACT TGTCACTACT TTCGGTTATG GTCTTCAATG CTTCGCCAGA TACCCAGATC 2761 ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 2821 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 2881 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 2941 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 3001 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG 3061 GAATCAAAGT Tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 3121 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 3181 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCT 3241 ACCAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 3301 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATgc CCGGTGGGTG 3361 AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC AACGCGCTGT 3421 ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 3481 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 3541 GCACCGATCG CCCTTCCCAA CAGTTGCGaA Ggtaagttta aacagatcca tactaactaa 3601 cttgttctga cataattttc agCtTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC 3661 CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG 3721 TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT CTACACCAAC GTGACCTATC 3781 CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA 3841 CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG 3901 TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT CGGTTACGGC CAGgtaagtt 3961 taattaagtt gataCTAACT AACAAGATCT GATTAATTTT CagGACAGTC GTTTGCCGTC 4021 TGAATTTGAC CTGAGCGCAT TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT 4081 GCGCTGGAGT GACGGCAGTT ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT 4141 CCGTGACGTC TCGTTGCTGC ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC 4201 TCGCTTTAAT GATGATTTCt ccCGCGCTGT ACTGGAGGCT GAAGTcCAGg taagtttaaa 4261 caggatctta ctaactaaca tgctaacact gaattttcag ATGTGCGGCG AGTTGCGTGA 4321 CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC 4381 GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG 4441 TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT 4501 GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT 4561 CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG 4621 AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT 4681 GGTGCAGGAT gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca 4741 gATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA 4801 TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT 4861 TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC 4921 GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT 4981 CTGGTCGCTG GGGAATGgta agtttaaaca gttgaatact aactaacgga gatctttgaa 5041 attttcagAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT 5101 CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT 5161 TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG 5221 GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATtC TTTGCGAGgt 5281 aagtttaaac agaactctac taactaacac attagatcct aattttcagT ACGCtCACGC 5341 GATGGGcAAC AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG 5401 TTTACAGGGC GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA 5461 CGGCAACCCG TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT 5521 CTGTATGAAC GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat 5581 aacctactaa ctaacgtaga taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA 5641 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 5701 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 5761 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 5821 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 5881 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCaTGG CAGCAGTGGA Ggtaagttta 5941 aacaagatcc tactaactaa ctctacattg atgaattttc agACTGGCGG AAAACCTCAG 6001 TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG ACCACCAGCG AAATGGATTT 6061 TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC CAGTCAGGCT TTCTTTCACA 6121 GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG CGCGATCAGT TCACCCGTGC 6181 ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC ATTGACCCTA ACGCCTGGGT 6241 CGgtaagttt aaacaaagtt gtactaacta acgaagatct tgataatttt cagAACGCTG 6301 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 6361 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 6421 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 6481 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 6541 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 6601 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 6661 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 6721 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 6781 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 6841 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 6901 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 6961 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt 7021 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG 7081 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 7141 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 7201 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 7261 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 7321 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT 7381 CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA 7441 ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA 7501 AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT 7561 GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT 7621 TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT 7681 TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG 7741 GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA 7801 GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 7861 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 7921 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 7981 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 8041 GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT 8101 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 8161 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 8221 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 8281 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 8341 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 8401 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 8461 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 8521 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 8581 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 8641 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 8701 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 8761 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 8821 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 8881 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 8941 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 9001 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 9061 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 9121 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 9181 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 9241 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 9301 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 9361 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 9421 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 9481 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 9541 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 9601 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 9661 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 9721 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 9781 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 9841 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 9901 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 9961 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC 10021 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA 10081 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC 10141 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC 10201 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 10261 TTTCACACAG GAAACAGCT // 1673 LOCUS ADDGENE_1673 6688 bp DNA circular SYN 01/03/2005 DEFINITION L4686 complete plasmid sequence KEYWORDS . SOURCE L4686 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1673 at www.addgene.org FEATURES Location/Qualifiers source 1..6688 /organism="Addgene plasmid L4686" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2450..2491 /label="SynIVS.A" CDS 2525..3562 /label="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 2525..2551 /label="SV40.NLS" misc_feature 2567..2593 /label="SV40.NLS" misc_feature 2609..2635 /label="SV40.NLS" misc_feature 2651..2677 /label="SV40.NLS" misc_feature 2864..2914 /label="SynIVS.R" misc_feature 2939..2963 /label="gfp.Y66W" misc_feature 3069..3119 /label="SynIVS.S" misc_feature 3223..3247 /label="gfp.N146I" misc_feature 3242..3265 /label="gfp.M153T" misc_feature 3262..3283 /label="gfp.V163A" misc_feature 3284..3334 /label="SynIVS.T" misc_feature 3576..3626 /label="SynIVS.M" misc_feature 3758..3787 /label="let858.3'" promoter 4468..4496 /label="Amp_promoter" CDS 4538..5398 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4538..5398 /label="Ampicillin" /gene="Ampicillin" rep_origin 5553..6172 /label="pBR322_origin" misc_feature 6388..6414 /label="lacI.C" promoter 6481..6510 /label="lac_promoter" misc_feature 6545..6563 /label="M13r_primer" misc_feature 6577..6627 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCCCAAAG GACCCAAAGG TATGTTTCGA 2461 ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGAGGGTAC CGAGCTCAGA 2521 AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA 2581 GAAGAAGCGT AAGGTACCGG CAGAAAAAAT GACTGCTCCA AAGAAGAAGC GTAAGGTACC 2641 GGCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA 2701 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA 2761 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC 2821 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata 2881 tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTCATG 2941 GGGCGTTCAA TGCTTCTCGA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG 3001 TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA 3061 CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta aattttcagG 3121 TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA 3181 TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA TTTCACACAA 3241 CGTATACATC ACTGCCGACA AACAAAAGAA TGGGATCAAA GCGgtaagtt taaacatgat 3301 tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA 3361 AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC 3421 TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA 3481 CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG 3541 CATGGATGAA CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa 3601 ctaacgcgct ctatttaaat tttcagAATT CGCTAGCCGG CCATACAAGT AATCCGGATG 3661 ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT 3721 ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA 3781 AATCTAAaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg 3841 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat 3901 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc 3961 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga 4021 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg AGAATTGTCC 4081 TCGCTTGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4141 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4201 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4261 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4321 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4381 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4441 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4501 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 4561 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4621 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 4681 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 4741 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 4801 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 4861 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 4921 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 4981 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5041 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5101 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5161 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5221 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5281 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5341 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5401 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 5461 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5521 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 5581 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 5641 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5701 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 5761 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 5821 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 5881 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 5941 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6001 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6061 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6121 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6181 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6241 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6301 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6361 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6421 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 6481 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 6541 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 6601 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 6661 GGATACGCTA ACAACTTGGA AATGAAAT // 1674 LOCUS ADDGENE_1674 6688 bp DNA circular SYN 01/03/2005 DEFINITION L4687 complete plasmid sequence KEYWORDS . SOURCE L4687 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1674 at www.addgene.org FEATURES Location/Qualifiers source 1..6688 /organism="Addgene plasmid L4687" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2450..2491 /label="SynIVS.A" CDS 2525..3562 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPAEKMTAPKKKRKVPAEKMTAPKKKRKVPAEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 2525..2551 /label="SV40.NLS" misc_feature 2567..2593 /label="SV40.NLS" misc_feature 2609..2635 /label="SV40.NLS" misc_feature 2651..2677 /label="SV40.NLS" gene 2693..3553 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2864..2914 /label="SynIVS.R" misc_feature 2933..2962 /label="gfp.GLA" misc_feature 3069..3119 /label="SynIVS.S" misc_feature 3284..3334 /label="SynIVS.T" misc_feature 3435..3466 /label="gfp.T203Y" misc_feature 3576..3626 /label="SynIVS.M" misc_feature 3758..3787 /label="let858.3'" promoter 4468..4496 /label="Amp_promoter" gene 4538..5398 /label="Ampicillin" /gene="Ampicillin" CDS 4538..5398 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5553..6172 /label="pBR322_origin" misc_feature 6388..6414 /label="lacI.C" promoter 6481..6510 /label="lac_promoter" misc_feature 6545..6563 /label="M13r_primer" misc_feature 6577..6627 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCCCAAAG GACCCAAAGG TATGTTTCGA 2461 ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGAGGGTAC CGAGCTCAGA 2521 AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGCA GAAAAAATGA CTGCTCCAAA 2581 GAAGAAGCGT AAGGTACCGG CAGAAAAAAT GACTGCTCCA AAGAAGAAGC GTAAGGTACC 2641 GGCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA 2701 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA 2761 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC 2821 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata 2881 tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCGGTTA 2941 TGGTCTTCAA TGCTTCGCCA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG 3001 TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA 3061 CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta aattttcagG 3121 TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA 3181 TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA 3241 TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat 3301 tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA 3361 AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC 3421 TGTCCTTTTA CCAGACAACC ATTACCTGTC CTACCAATCT GCCCTTTCGA AAGATCCCAA 3481 CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG 3541 CATGGATGAA CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa 3601 ctaacgcgct ctatttaaat tttcagAATT CGCTAGCCGG CCATACAAGT AATCCGGATG 3661 ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT 3721 ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA 3781 AATCTAAaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg 3841 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat 3901 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc 3961 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga 4021 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg AGAATTGTCC 4081 TCGCTTGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4141 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4201 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4261 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4321 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4381 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4441 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4501 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 4561 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4621 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 4681 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 4741 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 4801 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 4861 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 4921 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 4981 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5041 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5101 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5161 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5221 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5281 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5341 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5401 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 5461 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5521 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 5581 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 5641 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5701 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 5761 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 5821 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 5881 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 5941 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6001 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6061 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6121 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6181 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6241 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6301 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6361 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6421 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 6481 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 6541 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 6601 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 6661 GGATACGCTA ACAACTTGGA AATGAAAT // 1675 LOCUS ADDGENE_1675 4650 bp DNA circular SYN 01/03/2005 DEFINITION L4752 complete plasmid sequence KEYWORDS . SOURCE L4752 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1675 at www.addgene.org FEATURES Location/Qualifiers source 1..4650 /organism="Addgene plasmid L4752" /mol_type="other DNA" misc_feature 77..118 /label="SynIVS.A" misc_feature 138..163 /label="pes10.UAS-270" misc_feature 385..407 /label="pes10.Tsa" promoter complement(453..471) /label="T7_promoter" CDS 488..1357 /label="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 659..709 /label="SynIVS.R" misc_feature 734..758 /label="gfp.Y66W" misc_feature 864..914 /label="SynIVS.S" misc_feature 1018..1042 /label="gfp.N146I" misc_feature 1037..1060 /label="gfp.M153T" misc_feature 1057..1078 /label="gfp.V163A" misc_feature 1079..1129 /label="SynIVS.T" misc_feature 1371..1421 /label="SynIVS.M" misc_feature 1498..1548 /label="SynIVS.L" promoter 2430..2458 /label="Amp_promoter" gene 2500..3360 /label="Ampicillin" /gene="Ampicillin" CDS 2500..3360 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3515..4134 /label="pBR322_origin" misc_feature 4350..4376 /label="lacI.C" promoter 4443..4472 /label="lac_promoter" misc_feature 4507..4525 /label="M13r_primer" misc_feature 4539..4589 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCggatc cccgggattg 61 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga 121 ggacccttgg agggtaccta atttgatcga taatatgtac atattttgtt attcgttccg 181 gtatcgtacc tccacctacc ataaatccca taccctacat ctttATCGAT TTTTTGCAAA 241 TTACGAGCGT TGTAGGGGGC GGAgCGATAG GTCCTATAGG TTTTgGTATA TCATCATTCA 301 TTCATTCATT GGTACATTCA TTTACCCACC TTCCTCTTTC TGAGCTTCTC TGGAGTTCTG 361 TGCTTCCTTT TTCCCTTATC TTTATACTGT AATTTTTAAC TTTCAGgcat tgattggatc 421 cTATCGATTC GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT 481 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 541 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 601 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 661 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 721 CACTACTTTC TCATGGGGCG TTCAATGCTT CTCGAGATAC CCAGATCATA TGAAACAGCA 781 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 841 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 901 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 961 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 1021 CTATATTTCA CACAACGTAT ACATCACTGC CGACAAACAA AAGAATGGGA TCAAAGCGgt 1081 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 1141 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 1201 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT 1261 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 1321 TGGGATTACA CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT gtaagtttaa 1381 actttctcat actaactaac gcgctctatt taaattttca gAATTCCAAC TGAGCGCCGG 1441 TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta 1501 agtttaaact gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC 1561 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 1621 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 1681 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 1741 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 1801 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 1861 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 1921 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 1981 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 2041 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 2101 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 2161 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 2221 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 2281 ctagtaaaaa ataagcaaag aaattaggcg aacgatgAGA ATTGTCCTCG CTTGGGCCCA 2341 AAAGGCCTTT TTTTTTTGGC GCCGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2401 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 2461 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2521 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 2581 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 2641 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 2701 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 2761 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2821 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 2881 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2941 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 3001 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 3061 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 3121 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 3181 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3241 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3301 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3361 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3421 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 3481 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 3541 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 3601 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 3661 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 3721 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 3781 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 3841 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3901 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3961 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 4021 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 4081 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 4141 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 4201 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4261 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4321 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4381 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4441 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 4501 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 4561 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 4621 ATGGATACGC TAACAACTTG GAAATGAAAT // 1676 LOCUS ADDGENE_1676 4650 bp DNA circular SYN 01/03/2005 DEFINITION L4753 complete plasmid sequence KEYWORDS . SOURCE L4753 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1676 at www.addgene.org FEATURES Location/Qualifiers source 1..4650 /organism="Addgene plasmid L4753" /mol_type="other DNA" misc_feature 77..118 /label="SynIVS.A" misc_feature 138..163 /label="pes10.UAS-270" misc_feature 385..407 /label="pes10.Tsa" promoter complement(453..471) /label="T7_promoter" CDS 488..1357 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 488..1348 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 659..709 /label="SynIVS.R" misc_feature 728..757 /label="gfp.GLA" misc_feature 864..914 /label="SynIVS.S" misc_feature 1079..1129 /label="SynIVS.T" misc_feature 1230..1261 /label="gfp.T203Y" misc_feature 1371..1421 /label="SynIVS.M" misc_feature 1498..1548 /label="SynIVS.L" promoter 2430..2458 /label="Amp_promoter" CDS 2500..3360 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2500..3360 /label="Ampicillin" /gene="Ampicillin" rep_origin 3515..4134 /label="pBR322_origin" misc_feature 4350..4376 /label="lacI.C" promoter 4443..4472 /label="lac_promoter" misc_feature 4507..4525 /label="M13r_primer" misc_feature 4539..4589 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCggatc cccgggattg 61 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga 121 ggacccttgg agggtaccta atttgatcga taatatgtac atattttgtt attcgttccg 181 gtatcgtacc tccacctacc ataaatccca taccctacat ctttATCGAT TTTTTGCAAA 241 TTACGAGCGT TGTAGGGGGC GGAgCGATAG GTCCTATAGG TTTTgGTATA TCATCATTCA 301 TTCATTCATT GGTACATTCA TTTACCCACC TTCCTCTTTC TGAGCTTCTC TGGAGTTCTG 361 TGCTTCCTTT TTCCCTTATC TTTATACTGT AATTTTTAAC TTTCAGgcat tgattggatc 421 cTATCGATTC GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT 481 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 541 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 601 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 661 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 721 CACTACTTTC GGTTATGGTC TTCAATGCTT CGCCAGATAC CCAGATCATA TGAAACAGCA 781 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 841 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 901 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 961 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 1021 CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt 1081 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 1141 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 1201 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCTACC AATCTGCCCT 1261 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 1321 TGGGATTACA CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT gtaagtttaa 1381 actttctcat actaactaac gcgctctatt taaattttca gAATTCCAAC TGAGCGCCGG 1441 TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta 1501 agtttaaact gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC 1561 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 1621 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 1681 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 1741 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 1801 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 1861 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 1921 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 1981 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 2041 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 2101 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 2161 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 2221 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 2281 ctagtaaaaa ataagcaaag aaattaggcg aacgatgAGA ATTGTCCTCG CTTGGGCCCA 2341 AAAGGCCTTT TTTTTTTGGC GCCGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2401 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 2461 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2521 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 2581 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 2641 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 2701 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 2761 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2821 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 2881 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2941 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 3001 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 3061 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 3121 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 3181 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3241 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3301 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3361 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3421 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 3481 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 3541 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 3601 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 3661 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 3721 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 3781 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 3841 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3901 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3961 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 4021 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 4081 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 4141 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 4201 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4261 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4321 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4381 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4441 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 4501 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 4561 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 4621 ATGGATACGC TAACAACTTG GAAATGAAAT // 1677 LOCUS ADDGENE_1677 7470 bp DNA circular SYN 01/03/2005 DEFINITION L4759 complete plasmid sequence KEYWORDS . SOURCE L4759 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1677 at www.addgene.org FEATURES Location/Qualifiers source 1..7470 /organism="Addgene plasmid L4759" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" CDS 3484..4434 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKSPQALEFASRPYK" gene 3526..4374 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3697..3747 /label="SynIVS.R" misc_feature 3769..3788 /label="gfp.S65C" misc_feature 3902..3952 /label="SynIVS.S" misc_feature 4117..4167 /label="SynIVS.T" misc_feature 4540..4569 /label="let858.3'" promoter 5250..5278 /label="Amp_promoter" gene 5320..6180 /label="Ampicillin" /gene="Ampicillin" CDS 5320..6180 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6335..6954 /label="pBR322_origin" misc_feature 7170..7196 /label="lacI.C" promoter 7263..7292 /label="lac_promoter" misc_feature 7327..7345 /label="M13r_primer" misc_feature 7359..7409 /label="decoy" misc_feature 7467..7490 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtgGTACC GAGCTCAGAA 3481 AAAATGACgG CaCCtAAaAA gAAACGCAAA GTACCGGTAG AAAAAATGAG TAAAGGAGAA 3541 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 3601 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 3661 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta 3721 actaaccctg attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 3781 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 3841 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 3901 Cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA 3961 GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA 4021 GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC 4081 ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta 4141 actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA 4201 AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT 4261 TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG 4321 AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAc 4381 GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC GGCCATACAA GTAATCCGGA 4441 TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC 4501 CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC 4561 CAAATCTAAa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc 4621 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga 4681 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat 4741 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata 4801 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT 4861 CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 4921 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 4981 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 5041 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 5101 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 5161 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 5221 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 5281 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 5341 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 5401 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 5461 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 5521 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 5581 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 5641 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 5701 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 5761 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 5821 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 5881 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 5941 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 6001 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 6061 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 6121 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 6181 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 6241 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 6301 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 6361 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 6421 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 6481 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 6541 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 6601 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 6661 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 6721 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 6781 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 6841 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 6901 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6961 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 7021 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 7081 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 7141 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 7201 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 7261 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 7321 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 7381 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 7441 ATGGATACGC TAACAACTTG GAAATGAAAT // 1678 LOCUS ADDGENE_1678 7601 bp DNA circular SYN 01/03/2005 DEFINITION L4780 complete plasmid sequence KEYWORDS . SOURCE L4780 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1678 at www.addgene.org FEATURES Location/Qualifiers source 1..7601 /organism="Addgene plasmid L4780" /mol_type="other DNA" CDS 3012..4196 /label="Orf2" /translation="METDFFDGKSVLEIGFVTGLPSVYAFENGAEEIAMHTMDKTRGS PGLAKGPKGMFRMILT*HRTFSGGPLRVPREKMALLQSRLLLSAPRRAAATARASSVP VEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPV PWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFKSAMPEGYVQER TIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNIL GHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQL ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDEL YK" misc_feature 3169..3210 /label="SynIVS.A" misc_feature 3212..3228 /label="rf22.04" misc_feature 3240..3313 /label="ATG-MitLS" misc_feature 3498..3548 /label="SynIVS.R" misc_feature 3573..3597 /label="gfp.Y66W" misc_feature 3703..3753 /label="SynIVS.S" misc_feature 3857..3881 /label="gfp.N146I" misc_feature 3876..3899 /label="gfp.M153T" misc_feature 3896..3917 /label="gfp.V163A" misc_feature 3918..3968 /label="SynIVS.T" misc_feature 4210..4260 /label="SynIVS.M" misc_feature 4337..4387 /label="SynIVS.L" promoter 5515..5543 /label="Amp_promoter" gene 5585..6445 /label="Ampicillin" /gene="Ampicillin" CDS 5585..6445 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6600..7219 /label="pBR322_origin" misc_feature 7435..7461 /label="lacI.C" promoter 7528..7557 /label="lac_promoter" misc_feature 7592..7610 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGCATTC TATGAGAAAC GTCCACTGAT 61 TTGATTGAAT AAGACACGTG GTCATTCTTT GATTTTGAAA TTTCGCAAAT CCCTCTGATT 121 CATTTTTTTC TTTCAGTTGA TGGATAGATT TTCAAATTAA ATTTCCAGGT CCCCATATCA 181 TGCCATTCGA CAAGAATTCG TTATGCGATA ATAAGATAAC ACTCACTTTT GTTTGTTCGT 241 TGTTTTCGTA GTTTGGTGCA CACTGAGTAC GGTTGCCACG TCATTATGCA CCCACACCAA 301 AAGATGTCAT AGAAGATAGT ATTATCATAA CATTTGGGAA CATACGAGAA GACGACAATA 361 TAGATGGAGG TCCTGAAGAA CAGGTTCTTT TTAAAGAGAA GATTATTCAT AGAATGAAGG 421 AAATGAAAGT GTGATTGTTT GGACTAGATC AGATGTTGAT GTCATCATTT ATGCAAATCT 481 GAACACTCTC AGCTGTTATT ATGATTTCTT TACTAGATAT GATCATGCTG TGATTTTTGT 541 AGATGTGAAG TTGACTCAGT AATATTCAAT TGAAACGCAT GGCGCCACCT ACCTACCTGA 601 ATCCTTTTAT GGCAGTCACA GTTTTTGTAA CTACTTGATG GCCCAATTCT AGTTGTTTTA 661 TTATTATGGA ACTATTTCGC AAGTTGAAAA AATATTCCTA TAGAAATAGT TAGATTGAAT 721 TACGTCTAAA AATGTATTAG TAATTAGCTT GCTATTAATA ATGATATCAT CAGTCTGCTC 781 ACCTCAAACA TTGGTGTATT ATTCACGTGA ATCAGCAGCA AAAAAAGTTT AATAAGTTTA 841 ATTTTATTTA CCGAATACAC TGTTCTCTGT GTTTCTCACT GAAAATTTTC GCCTGGTACA 901 CGTGCCAGCC AGCCTCATTG GTCACCGATC ACACGTGAAT CATTGAGGTT CGTTTTTTAG 961 TGAAATAAAT AACAAAGCAA GGCGAAATAA AGTAAATAGG TTTGTTGAGA AATTAGTTGT 1021 GTTCGATGAT TTTTGGGAGT TAAACGTGAG ATGGTCAAGA AATAGTAACC AAGGATCTTT 1081 TACTGAAGCT GAGAGAAAAT AATCCATTCC AGAAACACTC GCAAAGGCTT TTTTTTGTCT 1141 AAGCTTAGAT GTTGGCTAAA CTCTGGAAAC CTCGAAATTT AGGTATTTTA ATTTGCAGCT 1201 TCTCGTGTAT TCAATCAGGA ATGAGCGGTG ATTATCGTTC GGCACGTTGA TTATCGGCCA 1261 GTTCGGAAAA TCGACAAATT ACAGGTTATC CGAATTTTTC GAAGTTTTGA ATAACCCGGT 1321 CTAGGTGAAA CCCTGAACTG ATCGCGGTGC CCTTTGGTGA ATGCCCAGTT ATTTGTGTTC 1381 ATCAGGAAGT TGACGAGAAT CTCAATTGAT ATTTCAACTT CTTCTAAAAA ACAATGTCTC 1441 TAAGCAACAG AAGTTCTCTA TAAAAAAGAT TAATCGTGAT CTGTTCAGTA GACACCGGTA 1501 CTATTTTTGC TAAGGAATCA TCTCTATCGT ATTACTTTGT GTTTACTTCA AGTTGATCGT 1561 TCTTTTCTAC AATGATCTAC CCTCTGCCAT CAATTTCATC AACTATCCAC CTACTCACAC 1621 TCTTTCATGG TACGTCATTG ATCTATTCAG GAACAAGAAT CGATCAGCTG TTCTCTTTTT 1681 CTTGCCATCA TTCTTCTTCT TATTCCTCTT CGCTGACCAC CAACCAGTGA AAAAAGAGAT 1741 GAAAAACAAA AAGTGTCGGA GCTCTCTGTT CTTTGAAGAT CATCCCTTTT ACCGCCAATC 1801 TTCTTTTCTT CTTCAGTGTC TTGTTTGATC TCTCACTTGG ATGAATAGAT TCTATCATCT 1861 CTAAACGTCT AACATCTCCA ATTGCTTCCG GCGTCTTCAT CTACTTCATC TAGCGAAGAC 1921 AAAAAGTAGG CGGAGTCAAT AGAAGGGGGT TAGGGTGGTA TTAAAGCAGC ATGGCATTTT 1981 CGCTTTTTCT CAAGAGCTCC ATTTTACTTT AAACTTATTC AATTAGTTTT TAGAGTTGAA 2041 GAACAAGCTC GACTAATATC TCAATTATTC TCCTGCAACT CAGATGGACT TTCACTTTTA 2101 TTTTAGCTTT AGATCTCACT TTCCTGACCT AGACATACAA ATTCCACACT CAATTCCAGT 2161 ACTCCAGTTC GTTCTTTGAT TCTTCCAAGT ACAATAGCTC TCTCTATGTT CGTGTTTATG 2221 ATTTTCCTGC CGTTGTTCTG CACACAACCC GCCCTCGTGA CTTTTCCATT CACTCCGCCC 2281 CTCTCTCTTT TGCGGGACAA TCTTTAAAAG CAACTGAATG GTCGAATGCA AACAAACCCT 2341 TTGGAAATTC TCTAGTTTTC GGTACAAACT TTTTCCTTCC TTTTTTTTCA AAATTTCAAC 2401 TTTCTGCCAT TCACGTCATG CGATCAAGAT TCACTCCATC CTAAAGCGTA TCTTATCTTC 2461 TTTGTTTTTG CCCCCTACCC ATTACTTTAT TTACTATCTA AAACTACAAA GAACCACCTG 2521 TCTCTTTAGT TTCGGGTCAT TTTTTTCAAA ATAAATAATA GCTCCGGCTC GGTTTCAGCG 2581 TTCTTCTCAT ATCCTTTCAT CACACAAAAA TGGTCCTTAC CATCCCAACA ATTGCTTCCG 2641 TTGCTCCAGT CAACTGTGAA CTCAGTTTGG ATTCCGGCCG TTGCATCAAG TACGTGGCAA 2701 CCAGAAATTC AAACCCGAAA AATCATGTGG CAGAGGAATT GCCTATAATG AGAAAAAATA 2761 GTCACCAAGG TACGGTTCAA TTTCAAATTT TTCCTGTTTG ATCAGAAACG TCAGTTTCGG 2821 CATGCCTCGA TAGCTCAGTT GGGAGAGCGT ACGACTGAAG ATCGTAAGGT CACCAGTTCG 2881 ATCCTGGTTC GGGGCATTGC TTTTTTAATT TTTGATTTAA TTTTGAAAAT GTCTTATTTT 2941 CAGATCATCA TGATGACTGG GAAGGTGTCA ACACAATTTG CCACACTATT GATAATATTG 3001 TCAATGTTGA GATGGAAACT GACTTTTTCG ATGGAAAATC TGTTTTGGAA ATCGGATTCG 3061 TAACTGGACT CCCATCAGTA TATGCTTTCG AAAATGGAGC AGAAGAAATT GCAATGCACA 3121 CAATGGATAA GACTAGAGGA TCCCCGGGAT TGGCCAAAGG ACCCAAAGgt atgtttcgaa 3181 tgatactaac ataacataga acattttcag GAGGACCCTT GAGGGTACct cgagaaaaaa 3241 tggcactcct gcaatcacgt ctcctgctat ccgcacctcg tcgtgccgct gcaactgcaa 3301 gagcttcatc cGTACCGGTA GAAAAAATGA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG 3361 TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG 3421 AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA 3481 AACTACCTGT TCCATGGgta agtttaaaca tatatatact aactaaccct gattatttaa 3541 attttcagCC AACACTTGTC ACTACTTTCT CATGGGGCGT TCAATGCTTC TCGAGATACC 3601 CAGATCATAT GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG 3661 AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC ACgtaagttt aaacagttcg 3721 gtactaacta accatacata tttaaatttt cagGTGCTGA AGTCAAGTTT GAAGGTGATA 3781 CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG 3841 GACACAAATT GGAATACAAC TATATTTCAC ACAACGTATA CATCACTGCC GACAAACAAA 3901 AGAATGGGAT CAAAGCGgta agtttaaaca tgattttact aactaactaa tctgatttaa 3961 attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC 4021 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC 4081 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGTCCTTC 4141 TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC AAATAGCATT 4201 CGTAGAATTg taagtttaaa ctttctcata ctaactaacg cgctctattt aaattttcag 4261 AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG 4321 GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa 4381 ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT 4441 ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT 4501 TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC 4561 AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA 4621 TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT 4681 CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT 4741 TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT 4801 CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA 4861 AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG 4921 ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA 4981 AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG 5041 AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC 5101 CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT 5161 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 5221 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 5281 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 5341 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT 5401 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 5461 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 5521 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 5581 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 5641 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 5701 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 5761 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 5821 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 5881 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 5941 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 6001 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 6061 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 6121 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 6181 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 6241 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 6301 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 6361 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 6421 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 6481 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 6541 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 6601 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 6661 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 6721 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 6781 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 6841 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 6901 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 6961 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 7021 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 7081 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 7141 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 7201 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 7261 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 7321 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 7381 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 7441 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 7501 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 7561 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1679 LOCUS ADDGENE_1679 7601 bp DNA circular SYN 01/03/2005 DEFINITION L4781 complete plasmid sequence KEYWORDS . SOURCE L4781 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1679 at www.addgene.org FEATURES Location/Qualifiers source 1..7601 /organism="Addgene plasmid L4781" /mol_type="other DNA" CDS 3012..4196 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="METDFFDGKSVLEIGFVTGLPSVYAFENGAEEIAMHTMDKTRGS PGLAKGPKGMFRMILT*HRTFSGGPLRVPREKMALLQSRLLLSAPRRAAATARASSVP VEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPV PWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQER TIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNIL GHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQL ADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDEL YK" misc_feature 3169..3210 /label="SynIVS.A" misc_feature 3212..3228 /label="rf22.04" misc_feature 3240..3313 /label="ATG-MitLS" gene 3327..4187 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3498..3548 /label="SynIVS.R" misc_feature 3567..3596 /label="gfp.GLA" misc_feature 3703..3753 /label="SynIVS.S" misc_feature 3918..3968 /label="SynIVS.T" misc_feature 4069..4100 /label="gfp.T203Y" misc_feature 4210..4260 /label="SynIVS.M" misc_feature 4337..4387 /label="SynIVS.L" promoter 5515..5543 /label="Amp_promoter" gene 5585..6445 /label="Ampicillin" /gene="Ampicillin" CDS 5585..6445 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6600..7219 /label="pBR322_origin" misc_feature 7435..7461 /label="lacI.C" promoter 7528..7557 /label="lac_promoter" misc_feature 7592..7610 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGCATTC TATGAGAAAC GTCCACTGAT 61 TTGATTGAAT AAGACACGTG GTCATTCTTT GATTTTGAAA TTTCGCAAAT CCCTCTGATT 121 CATTTTTTTC TTTCAGTTGA TGGATAGATT TTCAAATTAA ATTTCCAGGT CCCCATATCA 181 TGCCATTCGA CAAGAATTCG TTATGCGATA ATAAGATAAC ACTCACTTTT GTTTGTTCGT 241 TGTTTTCGTA GTTTGGTGCA CACTGAGTAC GGTTGCCACG TCATTATGCA CCCACACCAA 301 AAGATGTCAT AGAAGATAGT ATTATCATAA CATTTGGGAA CATACGAGAA GACGACAATA 361 TAGATGGAGG TCCTGAAGAA CAGGTTCTTT TTAAAGAGAA GATTATTCAT AGAATGAAGG 421 AAATGAAAGT GTGATTGTTT GGACTAGATC AGATGTTGAT GTCATCATTT ATGCAAATCT 481 GAACACTCTC AGCTGTTATT ATGATTTCTT TACTAGATAT GATCATGCTG TGATTTTTGT 541 AGATGTGAAG TTGACTCAGT AATATTCAAT TGAAACGCAT GGCGCCACCT ACCTACCTGA 601 ATCCTTTTAT GGCAGTCACA GTTTTTGTAA CTACTTGATG GCCCAATTCT AGTTGTTTTA 661 TTATTATGGA ACTATTTCGC AAGTTGAAAA AATATTCCTA TAGAAATAGT TAGATTGAAT 721 TACGTCTAAA AATGTATTAG TAATTAGCTT GCTATTAATA ATGATATCAT CAGTCTGCTC 781 ACCTCAAACA TTGGTGTATT ATTCACGTGA ATCAGCAGCA AAAAAAGTTT AATAAGTTTA 841 ATTTTATTTA CCGAATACAC TGTTCTCTGT GTTTCTCACT GAAAATTTTC GCCTGGTACA 901 CGTGCCAGCC AGCCTCATTG GTCACCGATC ACACGTGAAT CATTGAGGTT CGTTTTTTAG 961 TGAAATAAAT AACAAAGCAA GGCGAAATAA AGTAAATAGG TTTGTTGAGA AATTAGTTGT 1021 GTTCGATGAT TTTTGGGAGT TAAACGTGAG ATGGTCAAGA AATAGTAACC AAGGATCTTT 1081 TACTGAAGCT GAGAGAAAAT AATCCATTCC AGAAACACTC GCAAAGGCTT TTTTTTGTCT 1141 AAGCTTAGAT GTTGGCTAAA CTCTGGAAAC CTCGAAATTT AGGTATTTTA ATTTGCAGCT 1201 TCTCGTGTAT TCAATCAGGA ATGAGCGGTG ATTATCGTTC GGCACGTTGA TTATCGGCCA 1261 GTTCGGAAAA TCGACAAATT ACAGGTTATC CGAATTTTTC GAAGTTTTGA ATAACCCGGT 1321 CTAGGTGAAA CCCTGAACTG ATCGCGGTGC CCTTTGGTGA ATGCCCAGTT ATTTGTGTTC 1381 ATCAGGAAGT TGACGAGAAT CTCAATTGAT ATTTCAACTT CTTCTAAAAA ACAATGTCTC 1441 TAAGCAACAG AAGTTCTCTA TAAAAAAGAT TAATCGTGAT CTGTTCAGTA GACACCGGTA 1501 CTATTTTTGC TAAGGAATCA TCTCTATCGT ATTACTTTGT GTTTACTTCA AGTTGATCGT 1561 TCTTTTCTAC AATGATCTAC CCTCTGCCAT CAATTTCATC AACTATCCAC CTACTCACAC 1621 TCTTTCATGG TACGTCATTG ATCTATTCAG GAACAAGAAT CGATCAGCTG TTCTCTTTTT 1681 CTTGCCATCA TTCTTCTTCT TATTCCTCTT CGCTGACCAC CAACCAGTGA AAAAAGAGAT 1741 GAAAAACAAA AAGTGTCGGA GCTCTCTGTT CTTTGAAGAT CATCCCTTTT ACCGCCAATC 1801 TTCTTTTCTT CTTCAGTGTC TTGTTTGATC TCTCACTTGG ATGAATAGAT TCTATCATCT 1861 CTAAACGTCT AACATCTCCA ATTGCTTCCG GCGTCTTCAT CTACTTCATC TAGCGAAGAC 1921 AAAAAGTAGG CGGAGTCAAT AGAAGGGGGT TAGGGTGGTA TTAAAGCAGC ATGGCATTTT 1981 CGCTTTTTCT CAAGAGCTCC ATTTTACTTT AAACTTATTC AATTAGTTTT TAGAGTTGAA 2041 GAACAAGCTC GACTAATATC TCAATTATTC TCCTGCAACT CAGATGGACT TTCACTTTTA 2101 TTTTAGCTTT AGATCTCACT TTCCTGACCT AGACATACAA ATTCCACACT CAATTCCAGT 2161 ACTCCAGTTC GTTCTTTGAT TCTTCCAAGT ACAATAGCTC TCTCTATGTT CGTGTTTATG 2221 ATTTTCCTGC CGTTGTTCTG CACACAACCC GCCCTCGTGA CTTTTCCATT CACTCCGCCC 2281 CTCTCTCTTT TGCGGGACAA TCTTTAAAAG CAACTGAATG GTCGAATGCA AACAAACCCT 2341 TTGGAAATTC TCTAGTTTTC GGTACAAACT TTTTCCTTCC TTTTTTTTCA AAATTTCAAC 2401 TTTCTGCCAT TCACGTCATG CGATCAAGAT TCACTCCATC CTAAAGCGTA TCTTATCTTC 2461 TTTGTTTTTG CCCCCTACCC ATTACTTTAT TTACTATCTA AAACTACAAA GAACCACCTG 2521 TCTCTTTAGT TTCGGGTCAT TTTTTTCAAA ATAAATAATA GCTCCGGCTC GGTTTCAGCG 2581 TTCTTCTCAT ATCCTTTCAT CACACAAAAA TGGTCCTTAC CATCCCAACA ATTGCTTCCG 2641 TTGCTCCAGT CAACTGTGAA CTCAGTTTGG ATTCCGGCCG TTGCATCAAG TACGTGGCAA 2701 CCAGAAATTC AAACCCGAAA AATCATGTGG CAGAGGAATT GCCTATAATG AGAAAAAATA 2761 GTCACCAAGG TACGGTTCAA TTTCAAATTT TTCCTGTTTG ATCAGAAACG TCAGTTTCGG 2821 CATGCCTCGA TAGCTCAGTT GGGAGAGCGT ACGACTGAAG ATCGTAAGGT CACCAGTTCG 2881 ATCCTGGTTC GGGGCATTGC TTTTTTAATT TTTGATTTAA TTTTGAAAAT GTCTTATTTT 2941 CAGATCATCA TGATGACTGG GAAGGTGTCA ACACAATTTG CCACACTATT GATAATATTG 3001 TCAATGTTGA GATGGAAACT GACTTTTTCG ATGGAAAATC TGTTTTGGAA ATCGGATTCG 3061 TAACTGGACT CCCATCAGTA TATGCTTTCG AAAATGGAGC AGAAGAAATT GCAATGCACA 3121 CAATGGATAA GACTAGAGGA TCCCCGGGAT TGGCCAAAGG ACCCAAAGgt atgtttcgaa 3181 tgatactaac ataacataga acattttcag GAGGACCCTT GAGGGTACct cgagaaaaaa 3241 tggcactcct gcaatcacgt ctcctgctat ccgcacctcg tcgtgccgct gcaactgcaa 3301 gagcttcatc cGTACCGGTA GAAAAAATGA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG 3361 TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG 3421 AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA 3481 AACTACCTGT TCCATGGgta agtttaaaca tatatatact aactaaccct gattatttaa 3541 attttcagCC AACACTTGTC ACTACTTTCG GTTATGGTCT TCAATGCTTC GCCAGATACC 3601 CAGATCATAT GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG 3661 AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC ACgtaagttt aaacagttcg 3721 gtactaacta accatacata tttaaatttt cagGTGCTGA AGTCAAGTTT GAAGGTGATA 3781 CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG 3841 GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA 3901 AGAATGGAAT CAAAGTTgta agtttaaaca tgattttact aactaactaa tctgatttaa 3961 attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC 4021 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC 4081 TGTCCTACCA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGTCCTTC 4141 TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC AAATAGCATT 4201 CGTAGAATTg taagtttaaa ctttctcata ctaactaacg cgctctattt aaattttcag 4261 AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG 4321 GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa 4381 ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT 4441 ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT 4501 TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC 4561 AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA 4621 TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT 4681 CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT 4741 TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT 4801 CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA 4861 AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG 4921 ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA 4981 AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG 5041 AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC 5101 CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT 5161 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 5221 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 5281 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 5341 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT 5401 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 5461 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 5521 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 5581 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 5641 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 5701 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 5761 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 5821 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 5881 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 5941 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 6001 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 6061 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 6121 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 6181 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 6241 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 6301 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 6361 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 6421 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 6481 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 6541 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 6601 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 6661 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 6721 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 6781 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 6841 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 6901 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 6961 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 7021 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 7081 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 7141 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 7201 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 7261 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 7321 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 7381 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 7441 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 7501 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 7561 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1680 LOCUS ADDGENE_1680 7515 bp DNA circular SYN 01/03/2005 DEFINITION L4796 complete plasmid sequence KEYWORDS . SOURCE L4796 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1680 at www.addgene.org FEATURES Location/Qualifiers source 1..7515 /organism="Addgene plasmid L4796" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" misc_feature 3484..3509 /label="SV40.NLS'''" CDS 3484..4479 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPSSEKMTAPKKKRKVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSPQALEFASRPYK" gene 3571..4419 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3742..3792 /label="SynIVS.R" misc_feature 3814..3833 /label="gfp.S65C" misc_feature 3947..3997 /label="SynIVS.S" misc_feature 4162..4212 /label="SynIVS.T" misc_feature 4585..4614 /label="let858.3'" promoter 5295..5323 /label="Amp_promoter" CDS 5365..6225 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5365..6225 /label="Ampicillin" /gene="Ampicillin" rep_origin 6380..6999 /label="pBR322_origin" misc_feature 7215..7241 /label="lacI.C" promoter 7308..7337 /label="lac_promoter" misc_feature 7372..7390 /label="M13r_primer" misc_feature 7404..7454 /label="decoy" misc_feature 7512..7535 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtgGTACC GAGCTCAGAA 3481 AAAATGACCG CTCCAAAGAA GAAACGCAAA GTACCGAGCT CAGAAAAAAT GACgGCaCCt 3541 AAaAAgAAAC GCAAAGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA 3601 GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT 3661 GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT 3721 GGAAAACTAC CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat 3781 ttaaattttc agCCAACACT TGTCACTACT TTCTgTTATG GTGTTCAATG CTTcTCgAGA 3841 TACCCAGATC ATATGAAACg GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA 3901 CAGGAAAGAA CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag 3961 ttcggtacta actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT 4021 GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT 4081 CTTGGACACA AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA 4141 CAAAAGAATG GAATCAAAGT Tgtaagttta aacatgattt tactaactaa ctaatctgat 4201 ttaaattttc agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA 4261 GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT 4321 TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC 4381 CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA TGGAcGAACT ATACAAAagc 4441 ccacaAGCTT TGGAATTCGC TAGCCGGCCA TACAAGTAAT CCGGATGATC GACGCCaACG 4501 TCGTTGAATT TTCAAATTTT AAATACTGAA TATTTGTTTT TTTTCCTATT ATTTATTTAT 4561 TCTCTTTGTG TTTTTTTTCT TGCTTTCTAA AAAATTAATT CAATCCAAAT CTAAacattt 4621 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca 4681 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg 4741 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat 4801 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa 4861 ctagtaaaaa ataagcaaag aaattaggcg aacgatgAGA ATTGTCCTCG CTTGGGCCCT 4921 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 4981 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 5041 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 5101 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 5161 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 5221 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 5281 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 5341 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 5401 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 5461 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 5521 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 5581 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 5641 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 5701 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 5761 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 5821 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 5881 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 5941 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 6001 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 6061 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 6121 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 6181 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 6241 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 6301 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 6361 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 6421 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 6481 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 6541 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 6601 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 6661 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 6721 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 6781 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 6841 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 6901 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6961 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 7021 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 7081 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 7141 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 7201 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 7261 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 7321 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 7381 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 7441 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 7501 ACTTGGAAAT GAAAT // 1681 LOCUS ADDGENE_1681 7560 bp DNA circular SYN 01/03/2005 DEFINITION L4809 complete plasmid sequence KEYWORDS . SOURCE L4809 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1681 at www.addgene.org FEATURES Location/Qualifiers source 1..7560 /organism="Addgene plasmid L4809" /mol_type="other DNA" misc_feature 2983..3009 /label="let858.enh<<" misc_feature 2994..3020 /label="let858.-455" misc_feature 3430..3453 /label="let858.Tsa" CDS 3484..4524 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPSSEKMTAPKKKRKVPSSEKMTAPKKKRKVPVEK MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWV SLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIF FKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHK LEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADH YQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKS PQALEFASRPYK" misc_feature 3529..3554 /label="SV40.NLS'''" gene 3616..4464 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3787..3837 /label="SynIVS.R" misc_feature 3859..3878 /label="gfp.S65C" misc_feature 3992..4042 /label="SynIVS.S" misc_feature 4207..4257 /label="SynIVS.T" misc_feature 4630..4659 /label="let858.3'" promoter 5340..5368 /label="Amp_promoter" CDS 5410..6270 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5410..6270 /label="Ampicillin" /gene="Ampicillin" rep_origin 6425..7044 /label="pBR322_origin" misc_feature 7260..7286 /label="lacI.C" promoter 7353..7382 /label="lac_promoter" misc_feature 7417..7435 /label="M13r_primer" misc_feature 7449..7499 /label="decoy" misc_feature 7557..7580 /label="let858.enh>>" ORIGIN 1 AAGCTTCTGG AGATTGCTCC AGCAGCTCTT AACAGTGTCT ACGACCGTCT TCGTGCAATT 61 CTCATGGAAA CTGAAAGATC GGAAAATGCA CTGGATCGAC GTATTCAGTA TATGATTGAG 121 ACTGCAATGC AGATTCGAAA GGACAAATTT GCGgtaaggt agaatatata aatagtttat 181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc 241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga 301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt 361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt 421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg 481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt 541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa 601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat 661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct 721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa 781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc 841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg 901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac 961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat 1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact 1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga 1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat 1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag 1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt 1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag 1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag 1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta 1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg 1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca 1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta 1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat 1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg 1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca 1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact 1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc 1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc 2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc 2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata 2161 attattgcag GCGTATCCGG CGGTCATTGA AGACTTGGAC TTGATTGAGG AGGAGGATCA 2221 GATCATCCAT ACACTTAATT TGGAGGATGC GGTTGATCCG GAAAATGGGC TTAgtaagtg 2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag ATGTGTTCAA 2341 ACTAGATCCA GAATTCGAAA AGAACGAGGA GGTTTATGAG GAGATCCGTA AGGAAATCAT 2401 TGGAAACGCC GATATTTCGG ATGAGGATGG TGGCGACGAG TTGGATGATG AAGAAGAGGG 2461 TAGTGATGTG GAAGAGGCTC CGAAGAAGAC TACAGAGATT ATTGATAATA CTGATCAGAA 2521 TTGACTGCTT TCAGAAGgta ttcattttga gttttgggcc ggcaaatctg taagttgccg 2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt 2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagAGAAG TCTACCTGAC AATGCAATCA 2701 TCTTTGGACT ACCAAGAAGC TGCTCACAAA TTGCTGAAAA TGAAGATTCC AGACAGCATG 2761 CAGgtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt 2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt 2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agAACGAACT 2941 CTGCGCGATG CTTGTCGATT GTTGTGCTCA ACAGCGTACC TACGAGCGAT TCTACGGaat 3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat 3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt 3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga 3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg 3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc 3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt 3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg 3421 agaaatttaa ttgtattcaa acttttttat agTAAGATac cggtgGTACC GAGCTCAGAA 3481 AAAATGACCG CCCCAAAGAA GAAACGCAAA GTACCGAGCT CAGAAAAAAT GACCGCTCCA 3541 AAGAAGAAAC GCAAAGTACC GAGCTCAGAA AAAATGACgG CaCCtAAaAA gAAACGCAAA 3601 GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT 3661 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 3721 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 3781 CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA 3841 ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC AGATCATATG 3901 AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 3961 TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa 4021 ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 4081 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 4141 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 4201 AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC 4261 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 4321 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 4381 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 4441 ACAGCTGCTG GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA 4501 TTCGCTAGCC GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA 4561 ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT 4621 TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg 4681 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc 4741 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga 4801 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg 4861 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag 4921 caaagaaatt aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT 4981 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 5041 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 5101 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 5161 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC 5221 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 5281 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 5341 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 5401 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 5461 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 5521 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 5581 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 5641 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 5701 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 5761 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 5821 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 5881 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 5941 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 6001 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 6061 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 6121 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 6181 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 6241 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 6301 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 6361 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 6421 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 6481 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 6541 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 6601 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 6661 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 6721 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 6781 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 6841 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 6901 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 6961 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 7021 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 7081 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 7141 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 7201 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 7261 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 7321 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 7381 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC 7441 GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA 7501 GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1682 LOCUS ADDGENE_1682 6755 bp DNA circular SYN 01/03/2005 DEFINITION L4816 complete plasmid sequence KEYWORDS . SOURCE L4816 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1682 at www.addgene.org FEATURES Location/Qualifiers source 1..6755 /organism="Addgene plasmid L4816" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" CDS 2481..3350 /label="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2652..2702 /label="SynIVS.R" misc_feature 2727..2751 /label="gfp.Y66W" misc_feature 2857..2907 /label="SynIVS.S" misc_feature 3011..3035 /label="gfp.N146I" misc_feature 3030..3053 /label="gfp.M153T" misc_feature 3050..3071 /label="gfp.V163A" misc_feature 3072..3122 /label="SynIVS.T" misc_feature 3364..3414 /label="SynIVS.M" misc_feature 3491..3541 /label="SynIVS.L" promoter 4669..4697 /label="Amp_promoter" CDS 4739..5599 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4739..5599 /label="Ampicillin" /gene="Ampicillin" rep_origin 5754..6373 /label="pBR322_origin" misc_feature 6589..6615 /label="lacI.C" promoter 6682..6711 /label="lac_promoter" misc_feature 6746..6764 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 2521 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 2581 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 2641 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 2701 agCCAACACT TGTCACTACT TTCTCATGGG GCGTTCAATG CTTCTCGAGA TACCCAGATC 2761 ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 2821 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 2881 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 2941 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 3001 AATTGGAATA CAACTATATT TCACACAACG TATACATCAC TGCCGACAAA CAAAAGAATG 3061 GGATCAAAGC Ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 3121 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 3181 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA 3241 CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 3301 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATAG CATTCGTAGA 3361 ATTgtaagtt taaactttct catactaact aacgcgctct atttaaattt tcagAATTCC 3421 AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC 3481 TGTCATCAGA gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca 3541 gCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC 3601 TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT 3661 CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT 3721 AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA 3781 TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA 3841 AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT 3901 TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC 3961 ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA 4021 TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG 4081 AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG 4141 GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG 4201 GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA 4261 TAACTGTTTC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 4321 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 4381 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 4441 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 4501 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 4561 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 4621 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 4681 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 4741 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 4801 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 4861 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 4921 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 4981 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 5041 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 5101 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 5161 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 5221 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 5281 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 5341 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 5401 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 5461 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 5521 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 5581 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 5641 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 5701 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 5761 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 5821 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 5881 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 5941 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 6001 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 6061 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 6121 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 6181 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 6241 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 6301 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 6361 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 6421 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 6481 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 6541 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 6601 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 6661 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 6721 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1683 LOCUS ADDGENE_1683 6755 bp DNA circular SYN 01/03/2005 DEFINITION L4817 complete plasmid sequence KEYWORDS . SOURCE L4817 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1683 at www.addgene.org FEATURES Location/Qualifiers source 1..6755 /organism="Addgene plasmid L4817" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" gene 2481..3341 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2481..3350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 2652..2702 /label="SynIVS.R" misc_feature 2721..2750 /label="gfp.GLA" misc_feature 2857..2907 /label="SynIVS.S" misc_feature 3072..3122 /label="SynIVS.T" misc_feature 3223..3254 /label="gfp.T203Y" misc_feature 3364..3414 /label="SynIVS.M" misc_feature 3491..3541 /label="SynIVS.L" promoter 4669..4697 /label="Amp_promoter" gene 4739..5599 /label="Ampicillin" /gene="Ampicillin" CDS 4739..5599 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5754..6373 /label="pBR322_origin" misc_feature 6589..6615 /label="lacI.C" promoter 6682..6711 /label="lac_promoter" misc_feature 6746..6764 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT 2461 GGAGGGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 2521 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 2581 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 2641 CTGTTCCATG Ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 2701 agCCAACACT TGTCACTACT TTCGGTTATG GTCTTCAATG CTTCGCCAGA TACCCAGATC 2761 ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA 2821 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa gtttaaacag ttcggtacta 2881 actaaccata catatttaaa ttttcagGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 2941 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 3001 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG 3061 GAATCAAAGT Tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 3121 agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 3181 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCT 3241 ACCAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 3301 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATAG CATTCGTAGA 3361 ATTgtaagtt taaactttct catactaact aacgcgctct atttaaattt tcagAATTCC 3421 AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC 3481 TGTCATCAGA gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca 3541 gCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC 3601 TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT 3661 CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT 3721 AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA 3781 TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA 3841 AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT 3901 TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC 3961 ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA 4021 TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG 4081 AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG 4141 GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG 4201 GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA 4261 TAACTGTTTC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 4321 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 4381 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 4441 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 4501 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 4561 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 4621 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 4681 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 4741 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 4801 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 4861 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 4921 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 4981 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 5041 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 5101 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 5161 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 5221 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 5281 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 5341 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 5401 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 5461 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 5521 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 5581 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 5641 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 5701 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 5761 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 5821 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 5881 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 5941 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 6001 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 6061 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 6121 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 6181 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 6241 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 6301 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 6361 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 6421 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 6481 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 6541 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 6601 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 6661 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 6721 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1684 LOCUS ADDGENE_1684 3498 bp DNA circular SYN 01/03/2005 DEFINITION L4819 complete plasmid sequence KEYWORDS . SOURCE L4819 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1684 at www.addgene.org FEATURES Location/Qualifiers source 1..3498 /organism="Addgene plasmid L4819" /mol_type="other DNA" promoter complement(12..31) /label="T3_promoter" misc_feature complement(48..66) /label="M13r_primer" promoter complement(101..130) /label="lac_promoter" misc_feature complement(197..223) /label="lacI.C" rep_origin complement(439..1058) /label="pBR322_origin" gene complement(1213..2073) /label="Ampicillin" /gene="Ampicillin" CDS complement(1213..2073) /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(2115..2143) /label="Amp_promoter" rep_origin complement(2336..2642) /label="f1_origin" misc_feature 2801..2817 /label="M13f20_primer" misc_feature complement(2660..2815) /label="lacZ_a" promoter 2828..2846 /label="T7_promoter" ORIGIN 1 CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT 61 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 121 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 181 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 241 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 301 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 361 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 421 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 481 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 541 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 601 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 661 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 721 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 781 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 841 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 901 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 961 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1021 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 1081 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 1141 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 1201 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 1261 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 1321 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 1381 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 1441 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 1501 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 1561 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 1621 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 1681 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 1741 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 1801 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 1861 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 1921 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 1981 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 2041 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 2101 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 2161 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTAAATTGT AAGCGTTAAT 2221 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC 2281 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT 2341 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA 2401 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG 2461 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA 2521 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT 2581 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT 2641 GCGCCGCTAC AGGGCGCGTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA 2701 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA 2761 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG 2821 CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGggtacCC GGGGATCCTC TAGAGTCGAC 2881 CTGCAGGCAT GCAAGCTtgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa 2941 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 3001 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 3061 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 3121 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 3181 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 3241 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 3301 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 3361 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 3421 caacttcttc gcccccgttt tcaccatgga accggtggat ccactagttc tagagcggcc 3481 gccaccgcgg tggagctc // 1685 LOCUS ADDGENE_1685 4676 bp DNA circular SYN 01/03/2005 DEFINITION L4842 complete plasmid sequence KEYWORDS . SOURCE L4842 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1685 at www.addgene.org FEATURES Location/Qualifiers source 1..4676 /organism="Addgene plasmid L4842" /mol_type="other DNA" promoter complement(1254..1273) /label="T3_promoter" misc_feature complement(1290..1308) /label="M13r_primer" promoter complement(1343..1372) /label="lac_promoter" misc_feature complement(1439..1465) /label="lacI.C" rep_origin complement(1681..2300) /label="pBR322_origin" gene complement(2455..3315) /label="Ampicillin" /gene="Ampicillin" CDS complement(2455..3315) /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(3357..3385) /label="Amp_promoter" rep_origin complement(3578..3884) /label="f1_origin" misc_feature 4043..4059 /label="M13f20_primer" misc_feature complement(3902..4057) /label="lacZ_a" promoter 4070..4088 /label="T7_promoter" ORIGIN 1 TCGAGGATGC CAAGAGACGC CAAGCCCAAA AGATCAACGA GCTCCAAGAG GCTCTTGATG 61 CCGCCAACTC TAAGAACGCT TCTCTTGAGA AGACCAAGTC CAGACTTGTC GGAGACTTGG 121 ACGACGCTCA AGTTGATGTC GAGAGAGCCA ACGGTGTCGC CAGTGCTCTT GAGAAGAAGC 181 AAAAGGGCTT CGACAAGATC ATCGACGAAT GGAGAAAGAA GACCGACGAC TTGGCCGCTG 241 AGCTTGATGG AGCTCAACGC GACCTTCGCA ACACCTCCAC TGATCTCTTC AAGGCCAAGA 301 ACGCCCAAGA GGAGCTCGCC GAGGTTGTTG AGGGACTCCG CCGTGAGAAC AAGAGCTTGA 361 GCCAAGAGAT CAAGGATCTT ACCGATCAAC TCGGAGAGGG AGGACGCTCT GTCCACGAAA 421 TGCAAAAGAT CATCCGCCGT CTTGAGATTG AGAAGGAAGA ACTCCAACAC GCTTTGGACG 481 AGGCTGAGGC TGCCCTTGAA GCTGAAGAGA GCAAGGTTCT CCGCGCCCAG GTTGAAGTTT 541 CCCAGATCCG TTCCGAAATC GAGAAACGCA TCCAGGAGAA GGAGGAAGAG TTCGAGAACA 601 CGAGAAAGAA CCACGCCCGC GCTCTTGAAT CAATGCAAGC Ttggcgagat tttcaggagc 661 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 721 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 781 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 841 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc 901 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca 961 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt 1021 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa 1081 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt 1141 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tggaaccggt 1201 ggatccacta gttctagagc ggccgccacc gcggtggagc tcCAGCTTTT GTTCCCTTTA 1261 GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 1321 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 1381 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 1441 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 1501 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 1561 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 1621 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 1681 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1741 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1801 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1861 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1921 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 1981 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 2041 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 2101 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 2161 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 2221 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 2281 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 2341 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 2401 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 2461 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 2521 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 2581 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 2641 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 2701 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 2761 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 2821 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 2881 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 2941 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 3001 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 3061 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 3121 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 3181 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 3241 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 3301 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 3361 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 3421 CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG 3481 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT 3541 TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT 3601 CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT 3661 GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA 3721 CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC 3781 GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA 3841 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG 3901 TCCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 3961 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 4021 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA 4081 CTATAGGGCG AATTGggtac ccggttccat ggtgaaaacg ggggcgaaga agttgtccat 4141 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa 4201 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc 4261 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga 4321 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 4381 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag 4441 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 4501 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 4561 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 4621 ctccatttta gcttccttag ctcctgaaaa tctcgccaAG CTTGCATGCC TGCAGG // 1686 LOCUS ADDGENE_1686 3410 bp DNA circular SYN 01/03/2005 DEFINITION L754 complete plasmid sequence KEYWORDS . SOURCE L754 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1686 at www.addgene.org FEATURES Location/Qualifiers source 1..3410 /organism="Addgene plasmid L754" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 127..144 /label="rf21.28" promoter 1324..1352 /label="Amp_promoter" CDS 1394..2254 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1394..2254 /label="Ampicillin" /gene="Ampicillin" rep_origin 2409..3028 /label="pBR322_origin" misc_feature 3244..3270 /label="lacI.C" promoter 3337..3366 /label="lac_promoter" misc_feature 3401..3419 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGCTAGCGTC GACGGTACCA TGGTATTGAT ATCTGAGCTC CGCATCGGCC 181 GCTGTCATCA GATCGCCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC 241 AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA 301 AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG 361 TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC 421 CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT 481 TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT 541 ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC 601 TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA 661 TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG 721 TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG 781 ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT 841 GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC 901 CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG 961 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 1021 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 1081 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 1141 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA 1201 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC 1261 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 1321 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 1381 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 1441 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA 1501 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 1561 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG 1621 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 1681 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 1741 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT 1801 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 1861 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 1921 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 1981 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 2041 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG 2101 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 2161 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 2221 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 2281 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT 2341 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 2401 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 2461 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 2521 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 2581 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 2641 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 2701 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 2761 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG 2821 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 2881 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 2941 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3001 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3061 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC 3121 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 3181 CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 3241 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT 3301 TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG 3361 TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1687 LOCUS ADDGENE_1687 3052 bp DNA circular SYN 01/03/2005 DEFINITION pAST19b complete plasmid sequence KEYWORDS . SOURCE pAST19b ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1687 at www.addgene.org FEATURES Location/Qualifiers source 1..3052 /organism="Addgene plasmid pAST19b" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..398) /label="lacZ_a" misc_feature complement(461..479) /label="M13r_primer" promoter complement(514..543) /label="lac_promoter" misc_feature complement(610..636) /label="lacI.C" rep_origin complement(852..1471) /label="pBR322_origin" gene complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" CDS complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 2522..2551 /label="SupTrp.5'" misc_feature 2566..2595 /label="SupTrp.3'" promoter complement(2894..2922) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGGAT 421 CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 481 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 541 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 601 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 661 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 721 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 781 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 1021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 1081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 1141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 1201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 1261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 1321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 1381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 1441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1561 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1621 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1681 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 1741 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 1801 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 1861 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 1921 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 1981 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2041 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2101 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2161 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2221 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2281 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2341 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2401 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2461 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATGACAAGA GAGAATAACA 2521 TGACTGCTTG GCGCAATGGT AGCGCGTTCG ACTCTAGATC GAAAGGTTGG GCGTTCGATC 2581 CGCTCAGTGG TCAAATTTTT TGTTTTTTAA GTAGTAATAT AATACAATTT AATTCAAAAT 2641 TACACGCAAA AATTTAATAA AGTAGTCCAA AATGCTAATC GTGTGAAAAA ATGCCTTCTG 2701 GTAATGTATT TCTCACACGA AAATTTGTTG AAATTGGTTT TTGACACAAG CGAATGTTGC 2761 AATTATTTTC TTGATTTTTC TACAAATTTG AAAATCAAAT TTCACGACTG CAAGTATAAC 2821 ATTAAAAACA ATATACCCAA GCCCACAAAA AACGTATTCT GAAAATTGTA TTATTGAAGC 2881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 3001 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1688 LOCUS ADDGENE_1688 4536 bp DNA circular SYN 01/03/2005 DEFINITION pBK125 complete plasmid sequence KEYWORDS . SOURCE pBK125 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1688 at www.addgene.org FEATURES Location/Qualifiers source 1..4536 /organism="Addgene plasmid pBK125" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..1233 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSGS PSEKMTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK QKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK RDHMVLLEFVTAAGITHGMDELYK" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 475..501 /label="SV40.NLS" gene 517..1224 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 709..728 /label="gfp.S65C" promoter 2450..2478 /label="Amp_promoter" CDS 2520..3380 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2520..3380 /label="Ampicillin" /gene="Ampicillin" rep_origin 3535..4154 /label="pBR322_origin" misc_feature 4370..4396 /label="lacI.C" promoter 4463..4492 /label="lac_promoter" misc_feature 4527..4545 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATcc tcatcggGAT CCCCctcaga aaaaatgact 481 gctccaaaga agaagcgtaa ggtaCCGGTA GAAAAAATGA GTAAAGGAGA AGAACTTTTC 541 ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT 601 GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC 661 ACTACTGGAA AACTACCTGT TCCATGGCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT 721 CAATGCTTcT CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG 781 CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA 841 CGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT AAAAGGTATT 901 GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA TAACTCACAC 961 AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTAACTT CAAAATTAGA 1021 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 1081 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 1141 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 1201 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTCCAA CTGAGCGCCG 1261 GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGATC 1321 GCCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG 1381 CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT 1441 TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA 1501 TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA 1561 ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT 1621 AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT 1681 TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT 1741 CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT 1801 ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC 1861 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC 1921 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT 1981 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC 2041 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 2101 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 2161 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 2221 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 2281 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 2341 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 2401 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 2461 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 2521 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 2581 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 2641 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 2701 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 2761 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 2821 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 2881 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 2941 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 3001 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 3061 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 3121 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 3181 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 3241 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 3301 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 3361 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 3421 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 3481 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 3541 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 3601 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 3661 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 3721 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 3781 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 3841 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 3901 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 3961 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 4021 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 4081 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 4141 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 4201 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 4261 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 4321 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 4381 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 4441 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 4501 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1689 LOCUS ADDGENE_1689 5843 bp DNA circular SYN 01/03/2005 DEFINITION pJKL464 complete plasmid sequence KEYWORDS . SOURCE pJKL464 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1689 at www.addgene.org FEATURES Location/Qualifiers source 1..5843 /organism="Addgene plasmid pJKL464" /mol_type="other DNA" misc_feature 1054..1080 /label="hlh8.-280" CDS 1334..2359 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSRGSPGLAKGPKGMFRMILT*HRTFSGGPLRVPSSEKMTAPKK KRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT GKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEG YVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKE DGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNLDLLTNGLYLNFQNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITH GMDELYK" misc_feature 1374..1415 /label="SynIVS.A" misc_feature 1417..1433 /label="rf22.04" misc_feature 1448..1474 /label="SV40.NLS" gene 1490..2350 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1661..1711 /label="SynIVS.R" misc_feature 1733..1752 /label="gfp.S65C" misc_feature 1866..1916 /label="SynIVS.S" misc_feature 2081..2131 /label="SynIVS.T.notcorrect" misc_feature 2445..2495 /label="SynIVS.L" promoter 3623..3651 /label="Amp_promoter" CDS 3693..4553 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3693..4553 /label="Ampicillin" /gene="Ampicillin" rep_origin 4708..5327 /label="pBR322_origin" misc_feature 5543..5569 /label="lacI.C" promoter 5636..5665 /label="lac_promoter" misc_feature 5700..5718 /label="M13r_primer" misc_feature 5732..5782 /label="decoy" ORIGIN 1 AAGCTTGAAA TCACAAATTC CATTTTTATG TCATACTTCA GATTTAAACA AATTTATTTT 61 ATGTGTGTTT TTTTAATGGT ATGTCCTAAC GATTTTCCTA ATTGACAACT ATTATAGATT 121 GAAAACACAG AATGCCAAGT AACGTAAGAA ATTTTTTTTT CGAATACCAG ACATAATTTC 181 AATAAAAGAA AAATCTTTAA AAAAAGTTTA ACTTTATACT ACAATAATTT TGGTTTTTAT 241 GAAGGAATCT GTATTGCGGC ACATCATGTA TTTGCTCAAG GTTTCGTTGG TTGACATATT 301 TTTGTGATCC GTGGAATGAG CTTGCATAGA GTTTAGTCAT AATTGGGTTT ATTTTACATA 361 CAGTTTGAAG TATGTTTTGT CTGTTATTCG AAATTTTTTA GTTAGTTTGG TAAGTTGAAA 421 AGAATTTACA AAATTTAACT AAACGAGGAC GCTCATCTCG ATTCTTATAA TTCCATAATA 481 ATTCTACGGT AAAAGTCAAG TTATGCCTCA AAACATGTAA TACATAATTA CCAAAACTAC 541 TTAATTACCG CATTTCCTTA GTATTTTAAT AGTGGTGTAG TCGAATTTTT TTTATTGCTT 601 TATTAGACTC AAAATTGTCT GCAAACACCC AATTTCATAA TGAAACTTAT TGAAAACAAT 661 ACACTTTGAA ACAATTTAGT ATTTCAGGAA TAAGGTCACT GGAACTTCGA AAATGATAAT 721 TTGAATAACC GTTAATAAAA TATTCAAACC AATTTGCATA ATCTTGATTT TGTATCATGA 781 TGGTATAGAA TGGGACTTTT GAAAGATGTG AAGTTTCAAT TCAGTAAGTA CAACTTTAAA 841 ATTGGGCTGC AAGATTTCTT CTTAAATTTT CAACAGTTTC AAAACACTGA AATCATGCTG 901 AACTATGGTT AGAGACTCGA CAACTTTCCT AAATTTTGGA GCAGTTTCAA TGGTTTTCGA 961 ATGTATACTA CATTTGTACC ATTTTATGTG TTAGTGTAGG TTGCTTTGCT TCTACCTTCA 1021 CTCTCAAATT CTTTTTTCCG CGGTAATTTT TCAACTATCG AAACAAAGAA GAAGTCATGA 1081 ATAACTGTCC GCATAGAGTT CTCACCCGTC CCCTTTCTTT TCACCGAGCA GTCACGAGAG 1141 AAAGAAGAAG AAGCGGACCG CTGCAGAGAT TCTTCGTAGC GGAGCCCCGC CCATCCTCTG 1201 TTACGCATAT GTTTGGATCT CATTTCTTCC ACTCATTAAC GTTCCAGGTA TGTTTTTTTT 1261 GTTGATTTTT TAATGTGTTA CTTTTCATAT GTTTATTACT TGAACTGACC GATTTCAAAT 1321 ATGATTTTCA CAGATGTCTA GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt 1381 tcgaatgata ctaacataac atagaacatt ttcagGAGGA CCCTTGAGGG TACCGAGCTC 1441 AGAAAAAATG ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTAGAAAAAA TGAGTAAAGG 1501 AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG 1561 GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT 1621 TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat 1681 actaactaac cctgattatt taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG 1741 TGTTCAATGC TTcTCgAGAT ACCCAGATCA TATGAAACgG CATGACTTTT TCAAGAGTGC 1801 CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA 1861 GACACgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGTGC 1921 TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT 1981 TAAAGAAGAT GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT 2041 ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT gtaagtttaa acttggactt 2101 actaactaac ggattatatt taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA 2161 TGGAAGCGTT CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT 2221 CCTTTTACCA GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA 2281 AAAGAGAGAC CACATGGTCC TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT 2341 GGATGAACTA TACAAATAGC ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC 2401 CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag 2461 ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT 2521 CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT 2581 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC 2641 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA 2701 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT 2761 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA 2821 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT 2881 CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG 2941 TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT 3001 TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC 3061 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG 3121 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT 3181 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA 3241 CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT 3301 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG 3361 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 3421 TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA 3481 CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA 3541 TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 3601 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 3661 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 3721 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 3781 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 3841 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 3901 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 3961 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 4021 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 4081 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 4141 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 4201 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 4261 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 4321 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 4381 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 4441 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 4501 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 4561 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 4621 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 4681 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 4741 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 4801 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 4861 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 4921 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 4981 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 5041 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 5101 ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 5161 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 5221 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 5281 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 5341 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 5401 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 5461 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC 5521 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 5581 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC 5641 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG 5701 GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct tactaactaa 5761 ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA 5821 CGCTAACAAC TTGGAAATGA AAT // 1690 LOCUS ADDGENE_1690 3488 bp DNA circular SYN 01/03/2005 DEFINITION pLT61 complete plasmid sequence KEYWORDS . SOURCE pLT61 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1690 at www.addgene.org FEATURES Location/Qualifiers source 1..3488 /organism="Addgene plasmid pLT61" /mol_type="other DNA" promoter 20..38 /label="T7_promoter" promoter complement(920..938) /label="T7_promoter" misc_feature 958..1106 /label="lacZ_a" misc_feature complement(949..965) /label="M13f20_primer" rep_origin 1124..1430 /label="f1_origin" promoter 1623..1651 /label="Amp_promoter" gene 1693..2553 /label="Ampicillin" /gene="Ampicillin" CDS 1693..2553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2708..3327 /label="pBR322_origin" ORIGIN 1 AACCTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCGGCAGAT CTGATATCAT 61 CGATGAATTC GAGCTCCACC GCGGTGgcgg ccgctctaga actagtggat cccccCCGAG 121 CACAATGACC TCAACGGAAT GTTTGTCGCT TCCAGAAGCG TTGGTAGCAG TAAGAGTGTA 181 CAAACCAGCA TGCTTGCGAG TTGCTCCACG GAGCACAAAT CGGGTCTTGT AGTCTTCGCT 241 TTCAATCCTG ATCTTTTGAT CATCGACTGG TTTCTCATTG AAGATCCATT CCTTCTTCGG 301 TGGTGGTTCT CCGCGTACTG GAACATCGAA TTCCACATCG TTTCCTACCT TGATAGTGAT 361 CGTCTTCATT GCTTCACGAT CAATCCATGG CTTCAAGTTT CTTGGCTTTG CAACAACCTT 421 TCGGCTTGGG TCTGAAGCCT CTCCCGGTCC GGCCTTGTTA ACAGCCTTCA CACGGAACTG 481 GTACTCTTCT CCCTCCTTGA GTCCAAGAAT CTCAGCATTG GTTTGATCTC CAGAAACCTT 541 TCCGCATTCT TGCCAGTCTC TTCCATGTTT GCCCTTCTTC TCAATGACGT ATTGAGTGAT 601 TGGAGCTCCT CCGTCAGACT TTGGTGGTTC CCATTCAAGT GAGACACGAT CAGCATCCCA 661 GTCGACAACA TCCGGAGTTC CGGTTTTTCC TGGCTCATCA TATGGATTCT TGGCTTTGGT 721 GTCCTTATCA GTAGACAATG CATCAGAAGC TCCTTCCTTA TTGACAGCCT TGACACGGAA 781 CTGATATGTT TGTCCCTTCT TGAGACCATC AATATGAGCC TTCGTATCCT TAACTTTGGC 841 GCATGGAACC CATCTTCCGA ATTcgatatc aagcttatcg ataccgtcga cctcgagGGG 901 GGGCCCGGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT 961 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1021 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1081 GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 1141 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 1201 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 1261 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 1321 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 1381 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 1441 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 1501 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 1561 TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 1621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 1681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 1741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 1801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 1861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 1921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 1981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 2041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 2101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 2161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 2221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 2281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 2341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 2401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 2461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 2521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 2581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 2641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 2701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 2761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 2821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 2881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 2941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 3001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 3061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 3121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 3181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 3241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 3301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 3361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 3421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 3481 GAGGAAGC // 1691 LOCUS ADDGENE_1691 3404 bp DNA circular SYN 01/03/2005 DEFINITION pLT63 complete plasmid sequence KEYWORDS . SOURCE pLT63 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1691 at www.addgene.org FEATURES Location/Qualifiers source 1..3404 /organism="Addgene plasmid pLT63" /mol_type="other DNA" promoter 20..38 /label="T7_promoter" promoter complement(836..854) /label="T7_promoter" misc_feature 874..1022 /label="lacZ_a" misc_feature complement(865..881) /label="M13f20_primer" rep_origin 1040..1346 /label="f1_origin" promoter 1539..1567 /label="Amp_promoter" gene 1609..2469 /label="Ampicillin" /gene="Ampicillin" CDS 1609..2469 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2624..3243 /label="pBR322_origin" ORIGIN 1 AACCTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCGGCAGAT CTGATATCAT 61 CGATGAATTC GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTggat ccaCCGGTGC 121 TCCACTATTA GAGGATACGG AATGCTGTGG AAAAGAGAGA TGTCAGCATG CGACTGAAGA 181 ATCTGAATAC AAGAAACTTG TATATGTAGC TGTGCATCTT GTGAATGTAC TCGAACGACT 241 ATCTCTTCCT TCTGCACATA TCGATGATTC CGATGAGGAA AAAGAGCCGA AAGCCGACGT 301 TCGAAGACTG ATGATCGTGT GCCATGAATT ACATATTCCA CTTCTACATC ATACACTTGA 361 AGAGAGGATT CCTGATAGTA ACAGTGCAGA GTTGGGACTT CCCAAAGCTG CAGTTTTGGA 421 ACAACTTCTA GAACTTGACC TAGATGTGAA TGCCACTGAT AAGAATAACG ACACTCCAAT 481 GCACATTCTG CTTCGAGCCA GAGAGTTTAG GAAAAGCTTG GTTCGAGCAC TGCTCGTACG 541 TGGCACATGG CTGTTCGCAA GGAATCGGCA CGGAGATGTG GTGTTGAATG TGATGAAACG 601 TATGAAAGCG TTGAATCACG CCAACTTTGA TGATCTACCA CTAGGTGCGT GTCGAATCAG 661 AAATTGAGAG TTATTAGATT ATTTGGAACG CGAATAACTT CATTATACCA CGGAGAGAGC 721 CAGAAATTGA AGAAATTGGT TAAGGAGAAT GGAATTTAAT GAATAATAGC GCACCAATTC 781 GATATCAAGC TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA ATTCGCCCTA 841 TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 901 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 961 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 1021 GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 1081 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 1141 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 1201 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 1261 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 1321 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 1381 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 1441 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT 1501 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG 1561 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 1621 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC 1681 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 1741 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 1801 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC 1861 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 1921 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC 1981 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG 2041 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 2101 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG 2161 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 2221 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG 2281 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 2341 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 2401 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG 2461 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 2521 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT 2581 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 2641 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 2701 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC 2761 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 2821 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 2881 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 2941 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 3001 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG 3061 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 3121 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 3181 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 3241 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG 3301 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC 3361 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGC // 1701 LOCUS ADDGENE_1701 5108 bp DNA circular SYN 12/31/1969 DEFINITION pPD177.62 complete plasmid sequence KEYWORDS . SOURCE pPD177.62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1701 at www.addgene.org FEATURES Location/Qualifiers source 1..5108 /organism="Addgene plasmid pPD177.62" /mol_type="other DNA" misc_feature 62..87 /label="pes10.UAS-270" misc_feature 309..331 /label="pes10.Tsa" misc_feature 371..412 /label="SynIVS.A" misc_feature 494..520 /label="SV40.NLS" CDS 494..2116 /label="Orf1" /translation="MTAPKKKRKVPARLDKSKVINSALELLNEVGIEGLTTRKLAQKL GVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRKFKHIVTN*PYT FKFSGNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYA LSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQ*V*TWRY*LTG SI*IFRGAEPAFLFGLELIICGLEKQLKCESGSTRKGEELFTGVVPILVELDGDVNGH KFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSW GVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKF SGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVS LNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSA LSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 542..571 /label="TetR.N" misc_feature 762..812 /label="TetR.IVS4" misc_feature 1114..1164 /label="TetR.IVS1" misc_feature 1418..1468 /label="SynIVS.R" misc_feature 1493..1517 /label="gfp.Y66W" misc_feature 1623..1673 /label="SynIVS.S" misc_feature 1777..1801 /label="gfp.N146I" misc_feature 1796..1819 /label="gfp.M153T" misc_feature 1816..1837 /label="gfp.V163A" misc_feature 1838..1888 /label="SynIVS.T" misc_feature 2130..2180 /label="SynIVS.M" misc_feature 2312..2341 /label="let858.3'" promoter 3022..3050 /label="Amp_promoter" CDS 3092..3952 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3092..3952 /label="Ampicillin" /gene="Ampicillin" rep_origin 4107..4726 /label="pBR322_origin" misc_feature 4942..4968 /label="lacI.C" promoter 5035..5064 /label="lac_promoter" misc_feature 5099..5117 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGtcga ctctagagga tccccgggta 61 cctaatttga tcgataatat gtacatattt tgttattcgt tccggtatcg tacctccacc 121 taccataaat cccataccct acatctttAT CGATTTTTTG CAAATTACGA GCGTTGTAGG 181 GGGCGGAgCG ATAGGTCCTA TAGGTTTTgG TATATCATCA TTCATTCATT CATTGGTACA 241 TTCATTTACC CACCTTCCTC TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT 301 TATCTTTATA CTGTAATTTT TAACTTTCAG gcattgattg gatccccggg attggccaaa 361 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc 421 ttggCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT GGAGGGTACC 481 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGCTA GATTAGATAA 541 AAGTAAAGTG ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC 601 AACCCGTAAA CTCGCCCAGA AGCTAGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA 661 AAATAAGCGG GCTTTGCTCG ACGCCTTAGC CATTGAGATG TTAGATAGGC ACCATACTCA 721 CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA AGATTTTTTA Cgtaagttta aacatattgt 781 tactaactaa ccttatacat ttaaattttc agGTAATAAC GCTAAAAGTT TTAGATGTGC 841 TTTACTAAGT CATCGCGATG GAGCAAAAGT ACATTTAGGT ACACGGCCTA CAGAAAAACA 901 GTATGAAACT CTCGAAAATC AATTAGCCTT TTTATGCCAA CAAGGTTTTT CACTAGAGAA 961 TGCATTATAT GCACTCAGCG CTGTGGGGCA TTTTACTTTA GGTTGCGTAT TGGAAGATCA 1021 AGAGCATCAA GTCGCTAAAG AAGAAAGGGA AACACCTACT ACTGATAGTA TGCCGCCATT 1081 ATTACGACAA GCTATCGAAT TATTTGATCA CCAgtaagtt taaacgtgga gatactaact 1141 aaccggcagt atttaaattt tcagAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT 1201 GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTACTA GAAAAGGAGA 1261 AGAACTCTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA 1321 CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA 1381 ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca tatatatact 1441 aactaaccct gattatttaa attttcagCC AACACTTGTC ACTACTTTCT CATGGGGCGT 1501 TCAATGCTTC TCGAGATACC CAGATCATAT GAAACAGCAT GACTTTTTCA AGAGTGCCAT 1561 GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC 1621 ACgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt cagGTGCTGA 1681 AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA 1741 AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC TATATTTCAC ACAACGTATA 1801 CATCACTGCC GACAAACAAA AGAATGGGAT CAAAGCGgta agtttaaaca tgattttact 1861 aactaactaa tctgatttaa attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG 1921 AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT 1981 TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA 2041 GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA 2101 TGAACTATAC AAATAGCATT CGTAGAATTg taagtttaaa ctttctcata ctaactaacg 2161 cgctctattt aaattttcag AATTCGCTAG CCGGCCATAC AAGTAATCCG GATGATCGAC 2221 GCCaACGTCG TTGAATTTTC AAATTTTAAA TACTGAATAT TTGTTTTTTT TCCTATTATT 2281 TATTTATTCT CTTTGTGTTT TTTTTCTTGC TTTCTAAAAA ATTAATTCAA TCCAAATCTA 2341 Aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca tctgaacaca 2401 atgtgcaagt ttatttatct tctcgctttc atttcattag gacgtggggg gaattggtgg 2461 aagggggaaa cacacaaaag gatgatggaa atgaaataag gacacacaat atgcaacaac 2521 attcaattca gaaatatgga ggaaggttta aaagaaaaca taaaaatata tagaggagga 2581 aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac gatgAGAATT GTCCTCGCTT 2641 GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 2701 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 2761 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 2821 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 2881 CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 2941 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 3001 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 3061 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 3121 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 3181 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 3241 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 3301 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 3361 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 3421 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 3481 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 3541 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 3601 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 3661 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 3721 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 3781 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 3841 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 3901 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 3961 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 4021 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 4081 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 4141 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 4201 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 4261 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 4321 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 4381 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4441 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4501 CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4561 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4621 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4681 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4741 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 4801 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 4861 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 4921 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 4981 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 5041 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 5101 AAACAGCT // 1702 LOCUS ADDGENE_1702 5108 bp DNA circular SYN 12/31/1969 DEFINITION pPD177.53 complete plasmid sequence KEYWORDS . SOURCE pPD177.53 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1702 at www.addgene.org FEATURES Location/Qualifiers source 1..5108 /organism="Addgene plasmid pPD177.53" /mol_type="other DNA" misc_feature 62..87 /label="pes10.UAS-270" misc_feature 309..331 /label="pes10.Tsa" misc_feature 371..412 /label="SynIVS.A" misc_feature 494..520 /label="SV40.NLS" CDS 494..2116 /label="Orf1" /translation="MTAPKKKRKVPARLDKSKVINSALELLNEVGIEGLTTRKLAQKL GVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRKFKRRSTN*RLG FKFSGNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYA LSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQ*V*TWRY*LTG SI*IFRGAEPAFLFGLELIICGLEKQLKCESGSTRKGEELFTGVVPILVELDGDVNGH KFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGY GLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKF SGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVS LNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSA LSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 542..571 /label="TetR.N" misc_feature 762..812 /label="Tet.IVS3" misc_feature 1114..1164 /label="TetR.IVS1" misc_feature 1418..1468 /label="SynIVS.R" misc_feature 1487..1516 /label="gfp.GLA" misc_feature 1623..1673 /label="SynIVS.S" misc_feature 1838..1888 /label="SynIVS.T" misc_feature 1989..2020 /label="gfp.T203Y" misc_feature 2130..2180 /label="SynIVS.M" misc_feature 2312..2341 /label="let858.3'" promoter 3022..3050 /label="Amp_promoter" gene 3092..3952 /label="Ampicillin" /gene="Ampicillin" CDS 3092..3952 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4107..4726 /label="pBR322_origin" misc_feature 4942..4968 /label="lacI.C" promoter 5035..5064 /label="lac_promoter" misc_feature 5099..5117 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGtcga ctctagagga tccccgggta 61 cctaatttga tcgataatat gtacatattt tgttattcgt tccggtatcg tacctccacc 121 taccataaat cccataccct acatctttAT CGATTTTTTG CAAATTACGA GCGTTGTAGG 181 GGGCGGAgCG ATAGGTCCTA TAGGTTTTgG TATATCATCA TTCATTCATT CATTGGTACA 241 TTCATTTACC CACCTTCCTC TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT 301 TATCTTTATA CTGTAATTTT TAACTTTCAG gcattgattg gatccccggg attggccaaa 361 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc 421 ttggCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG GAGGACCCTT GGAGGGTACC 481 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGCTA GATTAGATAA 541 AAGTAAAGTG ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC 601 AACCCGTAAA CTCGCCCAGA AGCTAGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA 661 AAATAAGCGG GCTTTGCTCG ACGCCTTAGC CATTGAGATG TTAGATAGGC ACCATACTCA 721 CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA AGATTTTTTA Cgtaagttta aacgtcgcag 781 tactaactaa cgtcttggat ttaaattttc agGTAATAAC GCTAAAAGTT TTAGATGTGC 841 TTTACTAAGT CATCGCGATG GAGCAAAAGT ACATTTAGGT ACACGGCCTA CAGAAAAACA 901 GTATGAAACT CTCGAAAATC AATTAGCCTT TTTATGCCAA CAAGGTTTTT CACTAGAGAA 961 TGCATTATAT GCACTCAGCG CTGTGGGGCA TTTTACTTTA GGTTGCGTAT TGGAAGATCA 1021 AGAGCATCAA GTCGCTAAAG AAGAAAGGGA AACACCTACT ACTGATAGTA TGCCGCCATT 1081 ATTACGACAA GCTATCGAAT TATTTGATCA CCAgtaagtt taaacgtgga gatactaact 1141 aaccggcagt atttaaattt tcagAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT 1201 GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTACTA GAAAAGGAGA 1261 AGAACTCTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA 1321 CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA 1381 ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca tatatatact 1441 aactaaccct gattatttaa attttcagCC AACACTTGTC ACTACTTTCG GTTATGGTCT 1501 TCAATGCTTC GCCAGATACC CAGATCATAT GAAACAGCAT GACTTTTTCA AGAGTGCCAT 1561 GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC 1621 ACgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt cagGTGCTGA 1681 AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA 1741 AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA 1801 CATCATGGCA GACAAACAAA AGAATGGAAT CAAAGTTgta agtttaaaca tgattttact 1861 aactaactaa tctgatttaa attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG 1921 AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT 1981 TTTACCAGAC AACCATTACC TGTCCTACCA ATCTGCCCTT TCGAAAGATC CCAACGAAAA 2041 GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA 2101 TGAACTATAC AAATAGCATT CGTAGAATTg taagtttaaa ctttctcata ctaactaacg 2161 cgctctattt aaattttcag AATTCGCTAG CCGGCCATAC AAGTAATCCG GATGATCGAC 2221 GCCaACGTCG TTGAATTTTC AAATTTTAAA TACTGAATAT TTGTTTTTTT TCCTATTATT 2281 TATTTATTCT CTTTGTGTTT TTTTTCTTGC TTTCTAAAAA ATTAATTCAA TCCAAATCTA 2341 Aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca tctgaacaca 2401 atgtgcaagt ttatttatct tctcgctttc atttcattag gacgtggggg gaattggtgg 2461 aagggggaaa cacacaaaag gatgatggaa atgaaataag gacacacaat atgcaacaac 2521 attcaattca gaaatatgga ggaaggttta aaagaaaaca taaaaatata tagaggagga 2581 aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac gatgAGAATT GTCCTCGCTT 2641 GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 2701 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 2761 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 2821 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 2881 CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 2941 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 3001 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 3061 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 3121 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 3181 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 3241 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 3301 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 3361 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 3421 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 3481 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 3541 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 3601 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 3661 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 3721 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 3781 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 3841 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 3901 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 3961 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 4021 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 4081 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 4141 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 4201 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 4261 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 4321 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 4381 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4441 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4501 CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4561 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4621 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4681 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4741 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 4801 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 4861 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 4921 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 4981 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 5041 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 5101 AAACAGCT // 1703 LOCUS ADDGENE_1703 3541 bp DNA circular SYN 06/27/2005 DEFINITION pPD107.09 complete plasmid sequence KEYWORDS . SOURCE pPD107.09 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1703 at www.addgene.org FEATURES Location/Qualifiers source 1..3541 /organism="Addgene plasmid pPD107.09" /mol_type="other DNA" misc_feature 291..320 /label="SupTrp.5'" misc_feature 335..364 /label="SupTrp.3'" promoter complement(696..714) /label="T7_promoter" misc_feature 734..882 /label="lacZ_a" misc_feature complement(725..741) /label="M13f20_primer" rep_origin 900..1206 /label="f1_origin" promoter 1399..1427 /label="Amp_promoter" gene 1469..2329 /label="Ampicillin" /gene="Ampicillin" CDS 1469..2329 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2484..3103 /label="pBR322_origin" misc_feature 3319..3345 /label="lacI.C" promoter 3412..3441 /label="lac_promoter" misc_feature 3476..3494 /label="M13r_primer" promoter 3511..3530 /label="T3_promoter" ORIGIN 1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc catggctagc 61 cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc 121 gacctcgagg gggggcccgG TACCGCATTT TCTCGCCATT CTCCtctttt aaaaattgag 181 agatcctttg agacggagag accgagagca ctctccccca tttctcacca ctctcccttc 241 tcctactgca tgtcttctat gtaaactgca tcctttcctc ttgttttcgt gactgcttgg 301 cgcaatggta gcgcgttcga ctcTagatcg aaaggttggg cgttcgatcc gctcagtggt 361 caaatgtttt gtttttgagc tttaagtttg gttctttcag ttatggtttt ttttgcagat 421 cacagtgtca aataaagcaa gattgttcgc agagcggctc aacaagcgtg ataggaaagg 481 aaagagtgaa attgagacat gccgacaagc tagatgtcct ccagAATTCT GGCAAGCGCT 541 CACTGagctc caccgcggtg gcggccgctc tagaactagt ggatccaccg gttccatggc 601 tagccacgtg acgcgtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac 661 cgtcgacctc gagggggggc ccggtaccCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC 721 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG 901 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 961 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 1021 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 1081 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 1141 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 1201 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 1261 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA 1321 TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 1381 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 1441 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 1501 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 1561 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 1621 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 1681 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 1741 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 1801 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 1861 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 1921 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 1981 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 2041 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 2101 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 2161 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 2221 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 2281 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 2341 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 2401 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 2461 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 2521 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 2581 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 2641 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 2701 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 2761 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 2821 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 2881 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 2941 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3001 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 3061 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 3121 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 3181 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 3241 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 3301 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 3361 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 3421 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 3481 CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT 3541 G // 1704 1705 LOCUS ADDGENE_1705 5930 bp DNA circular SYN 12/31/1969 DEFINITION pPD157.60 complete plasmid sequence KEYWORDS . SOURCE pPD157.60 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1705 at www.addgene.org FEATURES Location/Qualifiers source 1..5930 /organism="Addgene plasmid pPD157.60" /mol_type="other DNA" misc_feature 16..49 /label="unc25.-1.9k" misc_feature 1873..1904 /label="unc25.5'" promoter complement(1951..1969) /label="T7_promoter" CDS 1986..2894 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 1986..2834 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2157..2207 /label="SynIVS.R" misc_feature 2229..2248 /label="gfp.S65C" misc_feature 2362..2412 /label="SynIVS.S" misc_feature 2577..2627 /label="SynIVS.T" misc_feature 3000..3029 /label="let858.3'" promoter 3710..3738 /label="Amp_promoter" CDS 3780..4640 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3780..4640 /label="Ampicillin" /gene="Ampicillin" rep_origin 4795..5414 /label="pBR322_origin" misc_feature 5630..5656 /label="lacI.C" promoter 5723..5752 /label="lac_promoter" misc_feature 5787..5805 /label="M13r_primer" misc_feature 5819..5869 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGAGAAA TAAGAAATAA TTGTATAATT Tttttttcca aaaatttaaa 61 tttctaaact aaaattcgaa tttttgcatg caaaaaacac ccactttttg atctcaaatt 121 tgcacttatt aagcactaaa aaccaataat ttcttccctt taaaatctaa aaaaaatctt 181 ccaataaccc ccccactgat aacaacaaca aggcattttt cactcgacga ccggtggcgg 241 agcgcacgtc ttcttattct tcttcttctt cttcttttcc taccttttct atcccacttt 301 ttccccggaa ttttgagatt tgacatgatt tttcagagaa tttcgagttt tgagaaaaaa 361 aaaatcaaaa aagcgatttt ttggtcaaaa gccgaaattt aaagctagtt tttttggaaa 421 tatcgatttt ttttttgaga atttttcgat tttccaaaaa aaaaatcgaa agttgtttcc 481 cgaaaagccg aaattttttt tgagatatcg aaaaatcgga gaatttttcg atgtttccca 541 gaaatactaa aattttgaat tttccggaaa aaaaatcgtt aaaaaattag tttcttttaa 601 agaccgaaat ttaaagtttt ttttttggaa atatcgattt tttccgagat atctaaaatt 661 gaaagaaatt ttcgattttc tgagaaaaat acgaaaaaat cgaaaaaaaa acccaaattt 721 cggttttttc gtaaaaaaag tcgtaaaaat gtaatttttt tcctgaaaaa tcggaatttt 781 ttacaaaata tcggaaaata ctcaaaaaaa agctgaaaat ttcgattttc cggagaaaaa 841 atctttttaa aaaaatattt tttttttcag aaaataagaa aagccgaaat ttataactat 901 ttctccggaa attcgaaatt tttaacgaaa tatcggaata atttttagat ttttcaagtt 961 tgactttgcg agaaaaaaat cggaaaaacc tcgattcgac gccgaaaaat gctccttttc 1021 gaaaagattt ttgaaatttc agaaaatcga catgcaagcg cgctccacgg cgaaatgaca 1081 acgatgatcc accgccctca aaaagttggg tctcgttagg tatttggcgg taaaactggt 1141 aaaactccag ttttgcctcc aacgagaccc aattttttgg ggcggtggtg gagcgcgctt 1201 gcacaagctg aaagcatttt tctgcgactc gataatattt tgaaaacctg tgtcaattct 1261 cgaaattctt tttttaaaaa ataatcccga gcttctctca gtcctcctct atgaggatgt 1321 tccttttttt tggtttttca atttttttta aaattccaaa tttctgttgt gcaattcact 1381 tccccccaag aaatcccaaa aatccccagt tttccccaaa aatgttccgt tttcatgtga 1441 tttttccccc atttttaaaa catttttttg actttttttt aaaatgatta ttattattgt 1501 tttctatttc atggccggta aattattttt tttctttctt tttttttgct cttttttttc 1561 aagaattttc gaattgtttg aagggctgct catctaatct tttgtcattt tgttctgatg 1621 ccatcatttc tgagaggacc tttgaagact cgtcacgaaa cgggaggggg gctcaagtga 1681 gcattattat tattattatt gtcgcaaaaa gtttaccccg ggctccccct ggctcccctc 1741 tttgagcaag ggtttaaggg ctcattttga tgacgaattg ctcattggga ttatagtcac 1801 gcccctcttt tggagcaact acacaactga gccacagtaa tccttggggg cggggtcagt 1861 aggaccccct ccggaatagg gAAAAGCTCA GTTCACCGCC AAAAATATCT AGAGGATCCT 1921 ATCGATTCGC GGCCGCTGTA CACCCGGGTG CCCTATAGTG AGTCGTATTG GTACCGGTAG 1981 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 2041 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 2101 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 2161 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 2221 CTACTTTCTg TTATGGTGTT CAATGCTTcT CGAGATACCC AGATCATATG AAACAGCATG 2281 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 2341 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 2401 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 2461 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 2521 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 2581 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 2641 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 2701 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 2761 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 2821 GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC 2881 GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA 2941 CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT 3001 TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg tctcccaatt 3061 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat 3121 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat 3181 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa 3241 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt 3301 aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 3361 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 3421 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 3481 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 3541 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 3601 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 3661 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 3721 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 3781 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 3841 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 3901 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 3961 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 4021 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 4081 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 4141 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 4201 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 4261 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 4321 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 4381 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 4441 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 4501 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 4561 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 4621 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 4681 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 4741 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 4801 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 4861 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 4921 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 4981 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 5041 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 5101 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 5161 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 5221 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 5281 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 5341 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 5401 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 5461 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 5521 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 5581 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 5641 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 5701 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 5761 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 5821 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 5881 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1706 LOCUS ADDGENE_1706 6943 bp DNA circular SYN 12/31/1969 DEFINITION pPD160.60 complete plasmid sequence KEYWORDS . SOURCE pPD160.60 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1706 at www.addgene.org FEATURES Location/Qualifiers source 1..6943 /organism="Addgene plasmid pPD160.60" /mol_type="other DNA" misc_feature 37..69 /label="unc30.-2.9k" misc_feature 2883..2914 /label="unc30.5'" promoter complement(2964..2982) /label="T7_promoter" CDS 2999..3907 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 2999..3847 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3170..3220 /label="SynIVS.R" misc_feature 3242..3261 /label="gfp.S65C" misc_feature 3375..3425 /label="SynIVS.S" misc_feature 3590..3640 /label="SynIVS.T" misc_feature 4013..4042 /label="let858.3'" promoter 4723..4751 /label="Amp_promoter" CDS 4793..5653 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4793..5653 /label="Ampicillin" /gene="Ampicillin" rep_origin 5808..6427 /label="pBR322_origin" misc_feature 6643..6669 /label="lacI.C" promoter 6736..6765 /label="lac_promoter" misc_feature 6800..6818 /label="M13r_primer" misc_feature 6832..6882 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGCTGCT GAAAACTTAG TTATGTaaaa attctgttgc tcgaaaactt 61 ccggaaaact ggaaaagagc gttccatatg cagagagagg cttagaaatt ttagaaactg 121 atattaattt tgagttcgcc attcggagca cgctcttcag cgcgtgcgaa cggctggtgc 181 tacagtatcg tagcgttttt tagtgaaatg gggaaaagtg aaaattgcta caacatcgta 241 gcgtttttgg gggaatggcc aaaaaatata acttcagcac gttctcaacc atgcgaaatc 301 agttgagaat tctgcgtctc ttctcccgca ttttttttag atctacggag atcaagccga 361 aatgagacac cacgtgggct agcaaagttg gagttcttgc ggaaaacttg caaacttcca 421 gctaaaaatc tccaaaaata aatactcaat ccaaattcac aaccctccaa aaatgccctg 481 tttcctcctt ttttgtcctc ctttgctctt ccgctcgatg acactttttg atgtgttttg 541 gcagatggga gagaacgacg tcggcatatg ggacaagtta cgcaatcgga agaaagtgac 601 aaattaacgc ggttaagcgg ttttctcctg agagatgatt gaatttgata acaattttct 661 tgcaatactt tccccgctct cactctctga caaatgacat aaatgcggga aattgaacgg 721 aaattgggat atattggtga agagcacatt tctcaccttt agcaactttt tccacctttt 781 ttaaaacttt atttcttttt gtgttttgtc cttttttctg taattctgaa actgaataat 841 acattgttct tgatcggctc ttttgtttta ttgtcataaa tatcttgaaa gggcagatgg 901 tatttgatat tcaaacaata acatgttcac agacaggtgg aatttttgaa tgttattgat 961 tggaagtgaa atgagaagtt acacccaagt gggaacacgt tgaccgtgag aatttggctg 1021 gaagtggtta aaaaataatt gtaggtgatg ctgtgatatc gagacctagt gaaaacttaa 1081 acattcgatt ttatggaaga ataaatgctg gaaatgattt cattcattta aatctatttg 1141 agacttctct tgagataata cagatgttaa ataccatgtt ccactgaaaa atatacactt 1201 atgcagatta ggctttaatt taaataactg gcaaaactgc tgtaaaactt aaactctcaa 1261 aaaacatgtt catggaattt tttaaaaata acctatcata agcaacgttc ataatttttc 1321 tttcaaatgt ttatcaaata attaacccta atgtctaaag tattcaaact ttccttcgat 1381 ttatgtgaaa tcaattttag attataggtg gttataatga cttataacaa tccaaaacca 1441 tccaatatca acataaaaca ttcaaataaa ttttagattt ttcaaaattt ccggtcaaag 1501 tttttgcaaa ttggcaaaat tttgaaaaat ataagctttt gagaaaatct aaagaaatat 1561 cacatgttcc gacccccgac aatgttttaa tacaaataat taaaacaaaa ttacagtata 1621 aaaaatataa gaaaaacaat ttttttggtt gacttccaaa ttacaagtgg aaaaaactga 1681 gtaattgtca gtttttgaca gtaaatagaa aattttccaa aaatatgata agttttaaaa 1741 tgctattcgg tcattttgaa accatagggg tagtttttaa cactttcccc attggtgcta 1801 ctccgccttt aagcctcaat aaaactatca ttcaaataag aaagctttaa aaacattttc 1861 taataatttc ataaaacctt caaccccgtt ctaaaaattc tgtatttacg aaattttttg 1921 taaacagttt gcgcaattgc tcttcaaaaa agataacatc tggtgatgat aagggtagat 1981 gtgaaattca actctgtgaa cgaccggttg acctcgaaaa tttcatgtta tgttttacga 2041 ctatatcggt cagcacaact gaagatatac gtatgctctt ctgtaaaccc gagtttgtat 2101 agtgagaaca caaatgtttt tagtatatag agttaaaaat ttcatgagaa ttttacatac 2161 acccaatttc ttaggtttcc aagttttgca agttttaatc aaaacaatcg aaatttcaca 2221 ataagtattt attaaaacgt tctgaaacaa aaaaaacacc cccgatgtag ttttgatatt 2281 tttaagtgtt ttaaaaatca atagcactct caaaatttca aaagccacct ttaaaacatt 2341 acacaacacc caccaaaaac ttctgttcct taacctgttg gaaaagtgtt gtgctctgta 2401 ggtacgcatg taggcacgag gcaggcgcag gcatacaaaa aactatcatt ttctcacata 2461 aatccctgct cactgatcca taaaactacc gttcaaaccg attatctttt tcttcaatct 2521 ctgatatttt ccaattcttt cgcgcgctac ttcatcattt ttacccatta gacaccgtag 2581 cccgtttcat ttcaaaacat cttttccttc tttcccttct ttttcttgtc ggtatgactc 2641 taatccctcc attttgatgc atcatcatac tctctctttt gcagtcagtc ggctgccatt 2701 taccatatgt gggtggcaca ctgggcgggg ctacagtagt cggcggagca ttctgaagag 2761 gggggaggag aagagcacac gaagaagaag agttgtcttc gaagagtctc gtctaggatc 2821 aaattctttg ccttcaatta atccgatcac tgactttgcg taagacggta ataatccttg 2881 gggattgagg cgccCTCCGG CAAAAGATCA ACACATAGAT TCTAGAGGAT CCTATCGATT 2941 CGCGGCCGCT GTACACCCGG GTGCCCTATA GTGAGTCGTA TTGGTACCGG TAGAAAAAAT 3001 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 3061 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 3121 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 3181 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 3241 CTgTTATGGT GTTCAATGCT TcTCGAGATA CCCAGATCAT ATGAAACAGC ATGACTTTTT 3301 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 3361 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 3421 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 3481 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 3541 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 3601 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 3661 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 3721 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 3781 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 3841 ACATGGCATG GAcGAACTAT ACAAAagccc acaAGCTTTG GAATTCGCTA GCCGGCCATA 3901 CAAGTAATCC GGATGATCGA CGCCaACGTC GTTGAATTTT CAAATTTTAA ATACTGAATA 3961 TTTGTTTTTT TTCCTATTAT TTATTTATTC TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA 4021 AATTAATTCA ATCCAAATCT AAacattttt ttttctcttt ccgtctccca attcgtattc 4081 cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta 4141 ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa 4201 ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac 4261 ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa 4321 cgatgAGAAT TGTCCTCGCT TGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA 4381 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 4441 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC 4501 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC 4561 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT 4621 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 4681 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 4741 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 4801 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 4861 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 4921 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 4981 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 5041 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 5101 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 5161 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 5221 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 5281 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 5341 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 5401 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 5461 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 5521 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 5581 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 5641 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 5701 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 5761 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 5821 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 5881 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 5941 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA 6001 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 6061 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 6121 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 6181 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA 6241 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 6301 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 6361 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 6421 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 6481 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC 6541 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC 6601 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG 6661 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA 6721 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG 6781 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta 6841 aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA 6901 ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1707 LOCUS ADDGENE_1707 6129 bp DNA circular SYN 12/31/1969 DEFINITION pPD159.88 complete plasmid sequence KEYWORDS . SOURCE pPD159.88 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1707 at www.addgene.org FEATURES Location/Qualifiers source 1..6129 /organism="Addgene plasmid pPD159.88" /mol_type="other DNA" misc_feature 1729..1759 /label="rol6.tsa" gene 1776..2636 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1776..2645 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 1947..1997 /label="SynIVS.R" misc_feature 2019..2038 /label="gfp.S65C" misc_feature 2152..2202 /label="SynIVS.S" misc_feature 2367..2417 /label="SynIVS.T" misc_feature 2731..2781 /label="SynIVS.L" promoter 3909..3937 /label="Amp_promoter" gene 3979..4839 /label="Ampicillin" /gene="Ampicillin" CDS 3979..4839 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4994..5613 /label="pBR322_origin" misc_feature 5829..5855 /label="lacI.C" promoter 5922..5951 /label="lac_promoter" misc_feature 5986..6004 /label="M13r_primer" misc_feature 6018..6068 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCCT CGAATTCTTA TCATCTTCGG 61 TTTTGATAAA ATTGTGGTGT AGTCCATAAT GAAATAAAAT TAGAAACAAT TGAAAATGAG 121 AATAATTGGA AGGAAAGAGA ACCGGTCATT TAGAATGAAC ACAATAGCTT TTTTAGTGAT 181 TGTTGCGTAA ATTTTATGAC ATTACGTCAT TGAATCCTGA AGAAGACAAT GTTTAAATGT 241 TCTCCAGATG GAATTAAATG TATAATTAAG CTCGAGATTT TTATCCGAGT TTTTATAAAA 301 GTTGGTGAGA CCCAGGATTA ATCAAAAACA AGTATTGACA GTTCCGTCAT TCATAACTCC 361 ACCGACTGCT CAAGTTGGCA AAAGTCAACT TTTAAAGTGG GTTATAAAGA GACTTCGATT 421 TTTCAGATTT GCAAATCATT TGAAAGCATT TGTTAAACTA GATATAAATA CATAGTTTTC 481 CTCAATCAGG AAAATCAAAC CACTGATCAT CTGATCAGCT GTCTTCTAAC AGGATGCACT 541 ATTAATGAAT TAAACGAACT CGGTCTATTC ACTCGGATAA TTGCAACAAT GTTAGTTCTA 601 TTTGATTTAT TTCGGCGCGA TCCGTAGCGA CACTTTCTTC CAGCAACCAT GTGATGCTGA 661 TTTCTATCAT CCGAAGAGAG AAGGAAACAT GGAGAGGAGC GGTTCAAGAA CAGGTGAATC 721 TGAACCTCCT TTTCCGTGTG TGTGGAAATA TACACAAACA AGTGACTGAT GAAGGCGGGA 781 AAAAGAAAAA CGATGGATTG AGTTATCTGG GTGAAGAATC TTAGACAAAG AGTTTGCAGA 841 AAAAGCTTGT TCTTGGAAAA ATAAAAAACT AGACAATTTT TAAAAAGGAA AACTACTTAT 901 TTATCACTTC AAAACCAGAA CCCTTTTGAC AATCTTTAAC TGTTTGAACT TCGTATTAGA 961 TCTCAGCAGC AAAATAATAG TTGAAAAAAT TTTATAAGAA AAGTTTTCGT GTTCAGTTGA 1021 AAACATCCAA AACAGAAAGT ATTTTTATTT ACTGATAATT CCTTAATACA CATCTATTAC 1081 CGTATTTGGG GCTTCTAAAA ATATATTCTA GTCGTTTCAA AGTTCAAGCA CATTACGTTA 1141 ATTCATAGAT CAAATCCACT AAACCTCAAC CATCACTTTT TCTAATTTCT TCCACTTCAA 1201 AAAAAAACTA ATCATCGTGG CTTCTTCCCG TTCCATGTCA TATGGTCAAC ATTAATCAGT 1261 CGATGGAATT GGCCTTTTTA CCGAAAATAC GAAGATTCCA CGCATCATTG CAACATTTTC 1321 GTCATAACCT CGCTAGCCCA AATCGGTTGT AGGATCCCCG AATCGCATTG TGGTTGAGCG 1381 TATGTCCTTC AAAAAGTCCA ACAAAAACTC GAATACCGTT TGCCATCGGA TGGTGTTGTT 1441 GAAGAGTTTT AACTATAAGC TACGCCCATC CGGACGTACG CGAGAAGCCC TTTCTGTTGA 1501 ATGGGTGGGC GGCGGAAGAA AAAACTTCTT AACACGGCTT TTCGGTTTTT CTACCCTATA 1561 TATTGGAGGA TAGGTGAAGG ATATTTTGGA GAATATACGA TTAGCGGCAA TCACAATGAC 1621 ATCCTTATGT ATATGCATTC TTTTTATTGT TTCCTGATAT TTATTCTCTC TTTATCATTT 1681 CACTTTTTCT TGGCACTCGT CCAAAAATCT CAATTAAAAA TTTCTGGATA TATCTTTAGA 1741 TCTAACTGAA AATTTCCAGG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA 1801 CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG 1861 TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA 1921 CTACTGGAAA ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg 1981 attatttaaa ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT 2041 CgAGATACCC AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT 2101 ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta 2161 aacagttcgg tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG 2221 AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA 2281 ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG 2341 ACAAACAAAA GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat 2401 ctgatttaaa ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC 2461 TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA 2521 ACCATTACCT GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA 2581 TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA 2641 AATAGCATTC GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG 2701 TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa actgagttct actaactaac 2761 gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC 2821 TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT 2881 CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA 2941 AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC 3001 CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT 3061 TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT 3121 ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG 3181 GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT 3241 TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC 3301 TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA 3361 GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG 3421 AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA 3481 CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG GCCGTACGGG CCCTTTCGTC 3541 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 3601 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 3661 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 3721 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC 3781 CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT 3841 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 3901 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 3961 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 4021 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 4081 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 4141 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 4201 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 4261 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 4321 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 4381 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 4441 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 4501 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 4561 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 4621 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 4681 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 4741 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 4801 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 4861 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 4921 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 4981 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 5041 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 5101 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 5161 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 5221 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 5281 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 5341 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 5401 CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 5461 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 5521 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 5581 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 5641 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 5701 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 5761 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 5821 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 5881 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 5941 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 6001 CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat tctcatttaa 6061 attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT AACAACTTGG 6121 AAATGAAAT // 1708 LOCUS ADDGENE_1708 7662 bp DNA circular SYN 12/31/1969 DEFINITION pPD191.45 complete plasmid sequence KEYWORDS . SOURCE pPD191.45 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1708 at www.addgene.org FEATURES Location/Qualifiers source 1..7662 /organism="Addgene plasmid pPD191.45" /mol_type="other DNA" misc_feature 3237..3278 /label="SynIVS.A" CDS 3309..4178 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 3309..4169 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 3480..3530 /label="SynIVS.R" misc_feature 3552..3571 /label="gfp.S65C" misc_feature 3685..3735 /label="SynIVS.S" misc_feature 3900..3950 /label="SynIVS.T" misc_feature 4264..4314 /label="SynIVS.L" promoter 5442..5470 /label="Amp_promoter" CDS 5512..6372 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5512..6372 /label="Ampicillin" /gene="Ampicillin" rep_origin 6527..7146 /label="pBR322_origin" misc_feature 7362..7388 /label="lacI.C" promoter 7455..7484 /label="lac_promoter" misc_feature 7519..7537 /label="M13r_primer" misc_feature 7551..7601 /label="decoy" ORIGIN 1 AAGCTTCAGT GCTGAAAAAA TTCAAgaact ctgttcaaag aaataattac atctaattaa 61 attttatttg tcttcttata aataaattgt tgtatatatc tcaagccttt tgttgatatt 121 taatccacag accatgatat tgatcatcta ttaattcttc gaacatttcc attcagaaca 181 cttttgtaac aatttaaatc gttttgaaat gaattcgatg tgtttatatt ttaatcagga 241 ggcgtttgga tttataaacg agcgtccgac aggattagtg tgccactttc tttttcagtc 301 cacacttgtc ccctctcaac acttttttgt tattctctca ctttatcctc acctctcgcc 361 taaagttcat ttcaaatttt tgattcattt cttactgtgt gaaatcaaga actagacgga 421 gtcggagtgg ttggctcgag atgcttcaat gagtcgacgg actgcgtcga agttactagt 481 ttttgtattt ttatgctctt tatgttttgg aactcaacgg tatgatatgc cgagaaaaat 541 tgacttgttt aatgatttga ttacacaatc gacaactcca gcatctccaa aggtgaataa 601 tcaaatatac aactttcaaa ccaaagcatt tataattttt gtagtttgtt ttagttttta 661 attttcaagt tttcttttga taattgttat tctaaaaaat tgaaagcaat gggtagtttt 721 gttcaaagtt caaatttata caacaacgtc tacctactaa aaaatggtat atgccgagtt 781 ttaagttgat tcgaaagttg atctcgtata aactagctga tctgaacttc cgaactcaaa 841 gtttttgccg aattagcacg aaacacaagt tcccaaaata tttcaaatgt tttcagtgcc 901 aatgtcttcc accaacaact ccatccaccc ctccaaactg tattccttac gattctcgat 961 tgcaagcagc aagtcttgaa gaagctattg tggcattccc agatcttacc attactcgac 1021 aagagaagac acaacaatct gtaattcttt ttttttcatt atgttttcgg atctcaatta 1081 actttcaaat tatggaaatt tgttttaaag ctttttcgga gtgtaaagta actataatat 1141 ttcagactgc aacactaaac aactgcaaaa ctaaacaatg ccgtgattgc tataaagatc 1201 tcagaagcca gttgcgaaaa gtaggaagcc tcttgcagaa tcgcttctag aaatgttcac 1261 ttccaatttt caggtcggtc ttcttccagg aaccattgac caggtgtttc acaatcagcg 1321 gaacttcaca acttgtcaaa aatatagatt tgcaagacag gataaaggag tctatgagaa 1381 gaaaaagaaa gccaaacaac attatgattg ggattatgta gaatgtaaga gaacattcag 1441 tgttcttttt ttggaaaaac tctcaagtac aaattgaaag gtttcagatg atgaagatga 1501 agatgatgat tacttttggg atggattatt ttggaagaag aagagaaatg tattaaagaa 1561 aattgtaaaa cgagacgttg aagctactac tgcaatttca caaccaccta acagtacagc 1621 aatgaactca accggaatta taggtttgta ttgaagacat aatttttttt ctaaaaactt 1681 cttattggta tatatttttc aacactctga aaataagata tattcaaaac aataatttta 1741 agtgaccgtc ataaataatt atgaaaagaa aacaaccatt caaaggtttt tttaaaaatt 1801 tttactcaaa atgcgtgtat tgttctccct caaaaaatta tttccgggtc gtgaattccc 1861 ttacgagaat ttcaattccc ctcgggtggt catcatctca aattccaatc ttctttttca 1921 ttttcaatct ctaacaattt caaattacta gaacccacaa accaatgttc gataacggca 1981 tgcaaccttg agagagtctc cgatttgatg tgtaaccttc attaaaccct gtaatacaag 2041 tcatgtcgac aaaccagatc ttcatccctt caacatttgg cagaaaagtg caattcgtct 2101 tttacaggaa ttcgatttcc gatctcttgc acaactcgag gcgtcactcc agatggatta 2161 ggaactgttt ccctttgtag tacatgttgg gtgtggcgac gtcttccgag tacctactat 2221 cctgcatatc ttaacgaagt cgtgtgcgat tatgcggaca catcgtgtct gagtggtagt 2281 gaaaagacag agaaagtggg ggaaaaagaa aataccattt tttcaggata cgcgtcatgc 2341 caaactggaa cccaacagtt gaatgtgcta cgaaatgaca gcgggaaatt gattccaata 2401 tcggtatcag ctggaatcaa ttgtgaatgt agattagcag ttggatcaac acttgaatca 2461 ctcgtacttg gacaaggaat ttccaaagca atgccgccaa ttgacacaac tagtacaaaa 2521 ccaccaaatt tggcaacaag cacaacaagc cattcctaat ttatttgggt attttactct 2581 tgataattgt attaatgatc atttaatgtt gttgaatatc acacatttaa catattcaat 2641 cgaagaaata taacgacgtt ccacaacatc cagtatacgt cttcctctgt attgagtttt 2701 attctttgac attgtgtcat gtttcagaac atagaaatat accaaagtgt tgagaagcat 2761 tgtataattc aattgaatct ttttcagttt acctcttctc ctttttcacc gtttccgtca 2821 tcaaaacgcc gttattcaag gtgagattat ggcgataaat gaatgccatt cacaaaattg 2881 caagacacta ttaaagaaga agaggtttag agatttgtac cgatcatagc ttcttctgag 2941 aaagcgacct tggcggccgt acttgaagag aagcaatttt tatcgccgta gaagatgttg 3001 cctgccaccc accctgtacg ttgttttttt gcttatcaac atctgaattt tgttgtcttt 3061 tgcaccgcct acttttacta tggaatttga ggaaatataa tatgaattga agagaatacg 3121 ctacctgtca aagaaacgtc acggttaggg tagtataaaa gggaagggta aCACTGAATA 3181 AGGTACCTGG CTTTCAGTCA TAGAGGGATC CCCGGGATTG GCCAAAGGAC CCAAAGgtat 3241 gtttcgaatg atactaacat aacatagaac attttcagGA GGACCCTTGG AGGGTACCGG 3301 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 3361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 3421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 3481 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 3541 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 3601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 3661 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 3721 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 3781 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 3841 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 3901 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 3961 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 4021 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 4081 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 4141 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG 4201 CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC 4261 AGAgtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC 4321 GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT 4381 CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT 4441 TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA 4501 ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC 4561 CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT 4621 TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT 4681 ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA 4741 TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT 4801 AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG 4861 CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG 4921 GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT 4981 GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT 5041 TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 5101 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 5161 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 5221 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 5281 GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT 5341 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA 5401 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 5461 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 5521 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 5581 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 5641 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 5701 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC 5761 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 5821 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 5881 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 5941 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 6001 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA 6061 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 6121 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 6181 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 6241 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 6301 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 6361 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 6421 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 6481 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 6541 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 6601 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 6661 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 6721 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 6781 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 6841 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 6901 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA 6961 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 7021 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 7081 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 7141 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 7201 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 7261 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 7321 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 7381 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 7441 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC 7501 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa 7561 acatgatctt actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA 7621 TCACTCACAA CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1709 LOCUS ADDGENE_1709 5090 bp DNA circular SYN 12/31/1969 DEFINITION pPD158.87 complete plasmid sequence KEYWORDS . SOURCE pPD158.87 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1709 at www.addgene.org FEATURES Location/Qualifiers source 1..5090 /organism="Addgene plasmid pPD158.87" /mol_type="other DNA" misc_feature 16..51 /label="sur5.-1.0k" misc_feature 1027..1058 /label="sur5.tsa" promoter complement(1111..1129) /label="T7_promoter" gene 1146..1994 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1146..2054 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" misc_feature 1317..1367 /label="SynIVS.R" misc_feature 1389..1408 /label="gfp.S65C" misc_feature 1522..1572 /label="SynIVS.S" misc_feature 1737..1787 /label="SynIVS.T" misc_feature 2160..2189 /label="let858.3'" promoter 2870..2898 /label="Amp_promoter" CDS 2940..3800 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2940..3800 /label="Ampicillin" /gene="Ampicillin" rep_origin 3955..4574 /label="pBR322_origin" misc_feature 4790..4816 /label="lacI.C" promoter 4883..4912 /label="lac_promoter" misc_feature 4947..4965 /label="M13r_primer" misc_feature 4979..5029 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGAGTGG AGAGTGTAGT CCCTCGTCGG ctctctgtgt atttctttca 61 atgcctgtgt ctcggtctat ctttgtctct atctgtttta aaagtttttt gcaaagtttc 121 aatttctttt ttattttctc ttcttttttt gcgcatttat tttttttaaa gagcgctgtt 181 cttcaattgg aaaacagtat aacgatcgaa ataaaaaaaa aacttgaaat agtatcggaa 241 ttgagatggg ggcaattgaa attgaagacg caacaaaaac atgtttttat cgtctgaaat 301 tatctaaact ttgaatctaa cgtgtcaatg gctactttta cagtagactt tttagagtaa 361 tgttaaaaag gataatatag gtttttcttc aaactatttt gtcacgccgc atatattctt 421 ttcatattcc attcgcaaag tgcatgatgt aacaaaaatg aaacgtcata attttttaat 481 ctttcacact ttttttgccg gccgttaaat caaaatcaaa tcaacttaat ccaacacgtg 541 cgggcttgca attttattac accggtgtcc tacagcgcca tgttatcttc tttgattaga 601 gatctctcac gcaacatttg acattgaaaa ttttgtctcc gaattgttgt gtacctactt 661 tgtcccgatg tcccaaattt tcactgataa caagagatat ctcaactgtt cttatcacgt 721 tgatacgtag aacgttttac actaaaccaa attgcagtcc ccttctacga accgtttgct 781 aattgttttg catagaacag tgatcaatgt gttgtgctca ctagtcaatg atgttcgtct 841 ctcatttttc ctacaacttc actgaaaagg ttttgaatat ctggtgattg taggcaatca 901 caccacagtt aatggcctgg caaataatga taagcgtgtg ataaacctca caaaaatacg 961 cctagaagtg gtgatatcgc tagttttgtg tcctttcgat ttttttcgtg ttgctttttt 1021 ctgtggataa acctttgaac tTTACATTTT GTTTTCAGAA TAACCGCTCT AGAGGATCCT 1081 ATCGATTCGC GGCCGCTGTA CACCCGGGTG CCCTATAGTG AGTCGTATTG GTACCGGTAG 1141 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 1201 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 1261 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 1321 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 1381 CTACTTTCTg TTATGGTGTT CAATGCTTcT CGAGATACCC AGATCATATG AAACAGCATG 1441 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 1501 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 1561 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 1621 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 1681 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 1741 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 1801 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 1861 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 1921 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 1981 GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA TTCGCTAGCC 2041 GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA 2101 CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT 2161 TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg tctcccaatt 2221 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat 2281 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat 2341 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa 2401 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt 2461 aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 2521 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 2581 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 2641 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 2701 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 2761 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 2821 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 2881 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 2941 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 3001 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 3061 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 3121 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 3181 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 3241 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 3301 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 3361 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 3421 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 3481 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 3541 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 3601 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 3661 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 3721 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 3781 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 3841 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 3901 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 3961 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 4021 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 4081 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 4141 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 4201 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 4261 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 4321 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 4381 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 4441 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 4501 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 4561 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 4621 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 4681 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 4741 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 4801 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 4861 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 4921 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 4981 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 5041 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1710 LOCUS ADDGENE_1710 4375 bp DNA circular SYN 12/31/1969 DEFINITION pPD157.63 complete plasmid sequence KEYWORDS . SOURCE pPD157.63 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1710 at www.addgene.org FEATURES Location/Qualifiers source 1..4375 /organism="Addgene plasmid pPD157.63" /mol_type="other DNA" misc_feature 212..243 /label="vit2.tsa" misc_feature 305..331 /label="T20G5.11.N" misc_feature 1574..1600 /label="T20G5.11.C" misc_feature 1730..1759 /label="let858.3'" promoter 2155..2183 /label="Amp_promoter" gene 2225..3085 /label="Ampicillin" /gene="Ampicillin" CDS 2225..3085 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3240..3859 /label="pBR322_origin" misc_feature 4075..4101 /label="lacI.C" promoter 4168..4197 /label="lac_promoter" misc_feature 4232..4250 /label="M13r_primer" misc_feature 4264..4314 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA gatcaaactg tattacttga aacaatttag 61 ttatatgttt agaacccctc attcaaaatt aatagacagg gctctcaccg aatgttgcaa 121 tttgtttctg ataagggtca caaagcggag cgaatgcttg aatgtgtcca tcaatgagct 181 tatcaatgcg ctaaaacgct ataacttcca tatgaagtca atcgaacata tgtcaatctt 241 tagccgtata taaaggtgca ctgaaaacag tccaatcacg gttcagccGG TACCGGTCGA 301 AAAAATGGAT TTAACCAAAC TAACGTTTGA AAGCGTTTTC GGTGGATCAG ATGTTCCTAT 361 GAAGCCTTCC CGATCGGAGG ATAACAAAAC GCCAAGAAAC AGAACAGATT TGGAGATGTT 421 TCTGAAGAAA ACTCCCCTCA TGGTACTAGA AGAGGCTGCT AAGGCTGTCT ATCAAAAGAC 481 GCCAACTTGG GGCACTGTCG AACTTCCTGA AGGCTTCGAG ATGACGTTGA TTCTGAATGA 541 AATTACTGTA AAAGGCCAGG CAACAAGCAA GAAAGCTGCG AGACAAAAGG CTGCTGTTGA 601 ATATTTACGC AAGGTTGTGG AGAAAGGAAA GCACGAAATC TTTTTCATTC CTGGAACAAC 661 CAAAGAAGAA GCTCTTTCGA ATATgtaatg ctttcaagtt tatcaaattg aattatattt 721 caattgtttt cagTGATCAA ATATCGGATA AGGCTGAGGA ATTGAAACGA TCAACTTCAG 781 ATGCTGTTCA GGATAACGAT AACGATGATT CGATTCCTAC AAGTGCTGAA TTTCCACCTG 841 GTATTTCGCC AACCGAGAAT TGGGTCGGAA AGTTGCAGGA AAAATCTCAA AAAAGCAAGC 901 TGCAAGCCCC AATCTATGAA GATTCCAAGA ATGAGAGAAC CGAGCGTTTC TTGGTTATAT 961 GCACGgtaga actgctaatt aataactcga aaataataaa aaatttcagA TGTGCAATCA 1021 AAAAACCAGA GGAATCAGAA GTAAGAAGAA GGACGCAAAG AATCTTGCAG CATGGTTGAT 1081 GTGGAAAGCG TTGGAAGACG GTATCGAATC TCTGGAATCA TATGATATGG gtatgcctcc 1141 aaataattgt agttaatatt taattttact gctttcagTT GATGTGATTG AAAATTTGGA 1201 AGAAGCTGAA CATTTACTCG AAATTCAGGA TCAAGCATCC AAGATTAAAG ACAAGCATTC 1261 CGCACTGATT GATATACTCT CGGACAAGAA AAGATTTTCA GACTACAGCA TGGATTTCAA 1321 CGTATTATCA GTGAGCACAA TGGGAATACA TCAGGTGCTA TTGGAAATCT CGTTCCGGCG 1381 TCTAGTTTCT CCAGACCCCG ACGATTTGGA AATGGGAGCA GAACACACCC AGACTGAAGA 1441 AATTATGAAG GCTACTGCCG AGAAGGAAAA GCTACGGAAG AAGAATATGC CAGATTCCGG 1501 GCCGCTAGTG TTTGCTGGAC ATGGTTCATC GGCGGAAGAG GCTAAACAGT GTGCTTGTAA 1561 ATCGGCGATT ATCCATTTCA ACACCTATGA TTTCACGGAT TGAGCTAGCC GGCCATACAA 1621 GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT 1681 GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT 1741 TAATTCAATC CAAATCTAAa catttttttt tctctttccg tctcccaatt cgtattccgc 1801 tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga 1861 cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga 1921 cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata 1981 aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga 2041 tgAGAATTGT CCTCGCTTGG GCCCAAAAGG CCTTTTTTTT TTGGCGCCGA CGTCAGGTGG 2101 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 2161 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 2221 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 2281 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 2341 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 2401 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 2461 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 2521 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 2581 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 2641 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 2701 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 2761 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 2821 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 2881 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 2941 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 3001 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 3061 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 3121 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 3181 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 3241 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 3301 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 3361 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 3421 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 3481 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 3541 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 3601 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 3661 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 3721 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 3781 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 3841 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 3901 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 3961 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 4021 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 4081 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 4141 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 4201 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 4261 GCTgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagAGCTTA 4321 AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1711 LOCUS ADDGENE_1711 5056 bp DNA circular SYN 12/31/1969 DEFINITION pPD158.117 complete plasmid sequence KEYWORDS . SOURCE pPD158.117 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1711 at www.addgene.org FEATURES Location/Qualifiers source 1..5056 /organism="Addgene plasmid pPD158.117" /mol_type="other DNA" CDS 898..2055 /label="Orf0" /translation="MILPLKMPTLKVMTQTSNCSKILLNYHLFLSFSLTVYFITNSSL SPSLSLSLSLFLFAHHLSFCPFLSLRPQRSPHSLASLFLDVFFFHLLQPFFAIFHLCQ SLRTTPIIDKISTVAANSCSPVATMRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGE GRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFV SLNGVLLTNK*HLNFQKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDG PVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDG*V*TISY*LTVEI*IFSGHYL VEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRHHLFL" misc_feature 1161..1191 /label="unc119.tsa" misc_feature 1276..1302 /label="RFP.N" misc_feature 1549..1599 /label="RFPivs.2" misc_feature 1627..1656 /label="RFP.V105A" misc_feature 1836..1886 /label="RFP.ivs4" misc_feature 1954..1983 /label="RFP.S197T" misc_feature 2025..2052 /label="RFP.C" misc_feature 2260..2289 /label="let858.3'" promoter 2970..2998 /label="Amp_promoter" gene 3040..3900 /label="Ampicillin" /gene="Ampicillin" CDS 3040..3900 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4055..4674 /label="pBR322_origin" misc_feature 4890..4916 /label="lacI.C" promoter 4983..5012 /label="lac_promoter" misc_feature 5047..5065 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCaag cttcagtaaa agaagtagaa ttttatagtt ttttttctgt 61 ttgaaaaatt ctccccatca atgttctttc aaataaatac atcactaatg caaagtattc 121 tataacctca tatctaaatt cttcaaaatc ttaacatatc ttatcattgc tttaagtcaa 181 acgtaacatt aaaaaaaatg ttttggaaaa tgtgtcaagt ctctcaaaat tcagtttttt 241 aaaccactcc tatagtccta tagtcctata gttacccatg aaatccttat atattactgt 301 aaaatgtttc aaaaaccatt ggcaaattgc cagaactgaa aatttccggc aaattgggga 361 accggcaaat tgccaatttg ctgaatttgc cggaaaaacg gtaattgccg aaagtttttg 421 acacgaaaat ggcaaattgt ggttttaaaa tttttttttt tggaaatttc agaatttcaa 481 ttttaatcgg caaaactgta ggcatcctaa gaatgttcct acatctattt tgaaaagtaa 541 gcgAATTaat tctatgaaaa tgtctaaaga aaatggggaa acaatttcaa aaaggcacag 601 tttcaagtgt ttccgaatta tactaaatcc ctctaaaaac ttccggcaaa ttgatatccg 661 taaaagagca aatccgcatt tttgccgaaa attaaaattt ccgacaaatc ggcaaaccgg 721 caatttggcg aaatttgccg gaaggcaatt gccgcccacc cctgttccag aggttcaaac 781 tggtagcaaa gctcaaaatt tctcaaattc tccaattttt ttttgaattt tggcagtgta 841 ccaaaatgac attcagtcat attggtttat tatagattta tttagataaa atcctaaatg 901 attctacctt taaagatgcc cactttaaaa gtaatgactc aaacttcaaa ttgctctaag 961 attctattga attaccatct tttcctctca ttttctctca ctgtctattt catcacaaat 1021 tcatccctct ctccctctct ttctctctcc ctctctctct ttctctttgc tcatcatctg 1081 tcattttgtc cgttcctctc tctgcgccct cagcgttccc cacactctct cgcttctctt 1141 ttcctagacg tcttcttttt tcatcttctt cagCCCTTTT TCGCCATTTT CCATCTCTGT 1201 CAATCATTAC GGACGACCCC CATTAtcgat aagatctcca cggtggccgc gaattcctgc 1261 agcccggtcg ccaccATGAG GTCTTCCAAG AATGTTATCA AGGAGTTCAT GAGGTTTAAG 1321 GTTCGCATGG AAGGAACGGT CAATGGGCAC GAGTTTGAAA TAGAAGGCGA AGGAGAGGGG 1381 AGGCCATACG AAGGCCACAA TACCGTAAAG CTTAAGGTAA CCAAGGGGGG ACCTTTGCCA 1441 TTTGCTTGGG ATATTTTGTC ACCACAATTT CAGTATGGAA GCAAGGTATA TGTCAAGCAC 1501 CCTGCCGACA TACCAGACTA TAAAAAGCTG TCATTTCCTG AAGGATTTgt aagtttaaac 1561 ggggtgttac taactaacaa ataacattta aattttcagA AATGGGAAAG GGTCATGAAC 1621 TTTGAAGACG GTGGCGTCGC TACTGTCACC CAAGATTCCA GCTTGCAAGA TGGCTGTTTC 1681 ATCTACAAGG TCAAGTTCAT TGGCGTGAAC TTTCCTTCCG ATGGACCTGT TATGCAAAAG 1741 AAGACAATGG GCTGGGAAGC CAGCACTGAG CGTTTGTATC CTCGTGATGG CGTGTTGAAA 1801 GGAGAGATTC ATAAGGCTCT GAAGCTGAAA GACGGgtaag tttaaactat atcttactaa 1861 ctaactgtag agatttaaat tttcagTGGT CATTACCTAG TTGAATTCAA AAGTATTTAC 1921 ATGGCAAAGA AGCCTGTGCA GCTACCAGGG TACTACTATG TTGATACCAA ACTGGATATC 1981 ACAAGCCACA ACGAAGACTA TACAATCGTT GAGCAGTATG AAAGAACCGA GGGACGCCAC 2041 CATCTGTTCC TTTAGcggcc gcgactctag aattccaact gagcgccggt cgctaccatt 2101 accaacttgt ctggtgtcaa aaataatagg cctaCTAGCC GGCCATACAA GTAATCCGGA 2161 TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC 2221 CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC 2281 CAAATCTAAa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc 2341 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga 2401 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat 2461 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata 2521 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgAGAATTGT 2581 CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 2641 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 2701 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 2761 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 2821 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 2881 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2941 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 3001 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 3061 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 3121 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 3181 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 3241 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 3301 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 3361 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 3421 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 3481 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 3541 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 3601 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 3661 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 3721 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3781 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3841 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3901 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3961 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 4021 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 4081 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 4141 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 4201 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 4261 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 4321 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 4381 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 4441 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 4501 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 4561 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 4621 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 4681 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 4741 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4801 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4861 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4921 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4981 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 5041 CACACAGGAA ACAGCT // 1712 LOCUS ADDGENE_1712 4394 bp DNA circular SYN 12/31/1969 DEFINITION pPD117.20 complete plasmid sequence KEYWORDS . SOURCE pPD117.20 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1712 at www.addgene.org FEATURES Location/Qualifiers source 1..4394 /organism="Addgene plasmid pPD117.20" /mol_type="other DNA" misc_feature 52..76 /label="unc37.-448" misc_feature 478..500 /label="unc37.Tsa" CDS 584..1492 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTLTYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASRPYK" gene 584..1432 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 755..805 /label="SynIVS.R" misc_feature 806..825 /label="gfp.F64L" misc_feature 827..846 /label="gfp.S65T" misc_feature 960..1010 /label="SynIVS.S" misc_feature 1175..1225 /label="SynIVS.T" misc_feature 1598..1627 /label="let858.3'" promoter 2308..2336 /label="Amp_promoter" gene 2378..3238 /label="Ampicillin" /gene="Ampicillin" CDS 2378..3238 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3393..4012 /label="pBR322_origin" misc_feature 4228..4254 /label="lacI.C" promoter 4321..4350 /label="lac_promoter" misc_feature 4385..4403 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTAGAGgatc ctcgtgttac 61 ttgaatatca aacttattgg atacattcat cttctacact cgggctcaaa agagctcaaa 121 acaaatgtgc tctatcgaca actcctcccc ccattcggtc acccattcag cgtcgcattc 181 atcgccgcgc gttcgtcctc ctcgacacca atacctcctt cattcttcaa cgttagtggg 241 gctatatgac cagtcgtttt gcaattaatg tatgattctc tccgtctaca ccactggaaa 301 cccatgtcct ctttctttgt ttcttcgtct cgttgtcaac gtctattcaa accacgtgct 361 atttcttgag ttttgttgtc ttctcccaat ccatcactag tctgttcttc cttgcatttt 421 tttgatcgaa tttttaaaac tcctctcata atttttatgt taataattca ctatccactt 481 gattgtattt aattttcagT AAAAATAAAG GCATCGGCTA GAGGATCCCC GGGATTGGCC 541 AAAGGACCCA AAGGAGGACC CTTGGAGGGT ACCGGTAGAA AAAATGAGTA AAGGAGAAGA 601 ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA 661 ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT 721 TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt ttaaacatat atatactaac 781 taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT ACTCTCACTT ATGGTGTTCA 841 ATGCTTCTCG AGATACCCAG ATCATATGAA ACAGCATGAC TTTTTCAAGA GTGCCATGCC 901 CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACg 961 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag GTGCTGAAGT 1021 CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA AAAGGTATTG ATTTTAAAGA 1081 AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT AACTCACACA ATGTATACAT 1141 CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt ttaaacatga ttttactaac 1201 taactaatct gatttaaatt ttcagAACTT CAAAATTAGA CACAACATTG AAGATGGAAG 1261 CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT 1321 ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG 1381 AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGAcGA 1441 ACTATACAAA agcccacaAG CTTTGGAATT CGCTAGCCGG CCATACAAGT AATCCGGATG 1501 ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT 1561 ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA 1621 AATCTAAaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg 1681 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat 1741 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc 1801 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga 1861 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg AGAATTGTCC 1921 TCGCTTGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 1981 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 2041 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 2101 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 2161 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 2221 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 2281 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 2341 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 2401 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 2461 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 2521 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 2581 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 2641 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 2701 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 2761 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 2821 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 2881 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 2941 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 3001 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 3061 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 3121 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 3181 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 3241 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 3301 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 3361 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3421 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3481 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3541 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3601 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3661 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3721 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3781 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3841 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3901 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3961 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4021 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 4081 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 4141 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 4201 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 4261 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 4321 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 4381 CACAGGAAAC AGCT // 1713 LOCUS ADDGENE_1713 8394 bp DNA circular SYN 12/31/1969 DEFINITION pPD157.99 complete plasmid sequence KEYWORDS . SOURCE pPD157.99 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1713 at www.addgene.org FEATURES Location/Qualifiers source 1..8394 /organism="Addgene plasmid pPD157.99" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" promoter complement(2440..2458) /label="T7_promoter" gene 2475..3323 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 2475..3551 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFASKPVTMQKSDEVTEKFKRYCNQLEKYGQTENVHSPVM AMLRRKYVLFCNKNLKYNSLSQKL" misc_feature 2646..2696 /label="SynIVS.R" misc_feature 2718..2737 /label="gfp.S65C" misc_feature 2851..2901 /label="SynIVS.S" misc_feature 3066..3116 /label="SynIVS.T" misc_feature 3381..3417 /label="smg5.N" CDS 4692..5351 /label="Orf2" /translation="MNHINSVFTSMSLTINPSISMTSFLLNGPISEPNVEFLSQLINY LVSVEFPPMEIIHDREESGPLLRRINQSEQKRLDIQIKTQNDEVNREDWRPVYVLMDY DVIVDKIRIALKIWDIDDFICILPSTVLDELDYQKTKNRAVRPVIRALMELQAEGKIV LKKCDNERSCAEQLVQSARRSAEDHKHIVAFLCKNPSEQKEMEGVTFYDIDQFYMKYL E" misc_feature 5313..5348 /label="smg5.C" misc_feature 5464..5493 /label="let858.3'" promoter 6174..6202 /label="Amp_promoter" gene 6244..7104 /label="Ampicillin" /gene="Ampicillin" CDS 6244..7104 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7259..7878 /label="pBR322_origin" misc_feature 8094..8120 /label="lacI.C" promoter 8187..8216 /label="lac_promoter" misc_feature 8251..8269 /label="M13r_primer" misc_feature 8283..8333 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGGTGC CCTATAGTGA GTCGTATTGG 2461 TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 2521 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 2581 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 2641 CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagCCAA 2701 CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC GAGATACCCA GATCATATGA 2761 AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT 2821 TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt actaactaac 2881 catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA 2941 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG 3001 AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA 3061 AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagAACT 3121 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA 3181 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT 3241 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA 3301 CAGCTGCTGG GATTACACAT GGCATGGAcG AACTATACAA AagcccacaA GCTTTGGAAT 3361 TCGCTAGCAA ACCGGTCACC ATGCAAAAGT CAGATGAAGT CACTGAAAAG TTCAAACGAT 3421 ATTGTAATCA ACTGGAAAAA TACGGCCAAA CTGAAAATGT GCATTCTCCA GTGATGGCGA 3481 TGTTGCGCAG AAAgtatgtt ttattctgta acaaaaactt aaaatacaac agcttgagtc 3541 agaaactatg aaattaataa acgatcttta agtttaaaat ttaaagaatt acaattttca 3601 gAGGCCGAAA ACAGCTCATT GAGATTATGA AACGCGACGG GGACTGTACA AGTTCAATCA 3661 ATAAATTGTG GATTGTTGGA TATTATCACC CTTTCCAGTT TTTTATTCGA gtaagattaa 3721 cttttagtca ttgtattatt tttatttgat ttccaaaggt tcttattaca gGATAAGGAG 3781 AAAAACATGG CAATAGCCGT GTTGCTGACA ATGTTCTGTG GAGAGCTTCA AGAAATGCTT 3841 TCGCTTCCCG ATGACAAATA CCCAGCATTG TGGAACATGT ACATTGGAGA TTTTCATCGT 3901 TATATGCCAG ACGAAGAAAT TCAGAAATGC TTGGCAGTTG GATATTATTC AAGAGCAATC 3961 GATCTAGATC CGAATCAAGG AAGAGCTTTC CACGTTCTCG CTGGTCTCCG AGCTGACCTG 4021 AATGTTGCAC AGAAGCTGAG ACTGATGATT TTAGGACAGC TCGCCGATGC TCCATATAAA 4081 AAAGGCACTG AACTCTTGGA ATATTTGAAA TTTCCACAAA AAGAATCCAC TGATAAATTG 4141 ATGGTAGATT TTGTCATTTG GGCATTGAAC GAGAAATCAA AACGAATGGA TTATCAGATG 4201 ACTGGAATCA AAATAGTAAA CGAGTTTAAA GCAGAAATCG AACAGAAACT CGAATTCGAT 4261 TGGTCTCTGA TCATGTCAAC GTGTCGTTTA GCATCTAAAT TGGCAATGAA GAAATTTGGA 4321 Tgtaagtacc gattccaata tctatagaaa attcttattc tgctgtagga taacataatt 4381 ttttcagTTC AACAATTCTA CAATTGCTTC GACACAATTT CAACGCTGTA TATTACAATT 4441 TATTCACGTA CGATTTCATC CAAATGCCTT CTAGCTGAAG CTATCTCATG GATTTCCGAT 4501 TCAGCCGAAA TACTCGGACA CTTGGATGAG CAGAAAAATG AGCCACATTT CCAGAAGCTA 4561 TCGGTTTTCG CCAAAACGgt aagatttaga gataagaaaa tgtagaatag atgtttcaat 4621 tttaaaatca aggggaatta attataaata taacttattt tcagAAATGG AACGAGCTCA 4681 ACGACTTGGT TATGAATCAT ATCAATTCCG TATTCACATC CATGTCACTC ACAATCAATC 4741 CATCTATTTC TATGACCAGC TTTCTGCTGA ATGGTCCAAT ATCCGAACCC AATGTTGAAT 4801 TTTTGAGTCA ATTAATCAAT TATCTGGTTT CCGTCGAATT CCCACCAATG GAGATAATCC 4861 ACGATAGAGA AGAATCGGGT CCATTGCTAC GACGAATTAA TCAATCGGAA CAAAAACGTT 4921 TGGATATTCA AATAAAGACA CAAAATGATG AAGTTAACCG GGAGGATTGG AGACCTGTTT 4981 ATGTATTGAT GGATTATGAC GTCATAGTTG ACAAAATAAG AATTGCTCTA AAAATTTGGG 5041 ATATCGATGA TTTCATCTGC ATTTTGCCAT CAACAGTGCT TGATGAACTT GATTATCAGA 5101 AAACGAAGAA TCGAGCAGTT CGTCCAGTAA TTCGGGCATT AATGGAGCTT CAAGCAGAGG 5161 GAAAAATTGT GCTAAAGAAA TGTGATAACG AGAGAAGCTG CGCGGAGCAA TTGGTTCAAA 5221 GTGCACGAAG ATCTGCAGAA GATCACAAGC ATATTGTCGC TTTTCTGTGC AAAAACCCGA 5281 GTGAACAGAA AGAAATGGAA GGAGTTACTT TTTACGATAT CGATCAGTTC TACATGAAGT 5341 ATTTGGAGTG ACCTAGGTTC CGGATGATCG ACGCCaACGT CGTTGAATTT TCAAATTTTA 5401 AATACTGAAT ATTTGTTTTT TTTCCTATTA TTTATTTATT CTCTTTGTGT TTTTTTTCTT 5461 GCTTTCTAAA AAATTAATTC AATCCAAATC TAAacatttt tttttctctt tccgtctccc 5521 aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt 5581 tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg 5641 aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt 5701 taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga 5761 aattaggcga acgatgAGAA TTGTCCTCGC TTGGGCCCTT TCGTCTCGCG CGTTTCGGTG 5821 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 5881 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 5941 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 6001 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 6061 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 6121 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 6181 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 6241 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 6301 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 6361 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 6421 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 6481 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 6541 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 6601 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 6661 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 6721 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 6781 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 6841 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 6901 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 6961 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 7021 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 7081 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 7141 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 7201 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 7261 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 7321 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 7381 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 7441 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 7501 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 7561 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 7621 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 7681 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 7741 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 7801 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 7861 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 7921 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 7981 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 8041 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 8101 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 8161 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 8221 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 8281 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 8341 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1714 LOCUS ADDGENE_1714 6420 bp DNA circular SYN 12/31/1969 DEFINITION pPD159.62 complete plasmid sequence KEYWORDS . SOURCE pPD159.62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1714 at www.addgene.org FEATURES Location/Qualifiers source 1..6420 /organism="Addgene plasmid pPD159.62" /mol_type="other DNA" misc_feature 26..49 /label="myo3.-2341" misc_feature 2345..2368 /label="myo3.Tsa" misc_feature 2441..2482 /label="SynIVS.A" CDS 2513..3292 /label="Orf1" /translation="MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFVSLNGVLLTNK *HLNFQKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGW EASTERLYPRDGVLKGEIHKALKLKDG*V*TISY*LTVEI*IFSGHYLVEFKSIYMAK KPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFL" misc_feature 2513..2539 /label="RFP.N" misc_feature 2786..2836 /label="RFPivs.2" misc_feature 2864..2893 /label="RFP.V105A" misc_feature 3073..3123 /label="RFP.ivs4" misc_feature 3262..3289 /label="RFP.C" misc_feature 3497..3526 /label="let858.3'" promoter 4207..4235 /label="Amp_promoter" CDS 4277..5137 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4277..5137 /label="Ampicillin" /gene="Ampicillin" rep_origin 5292..5911 /label="pBR322_origin" misc_feature 6127..6153 /label="lacI.C" promoter 6220..6249 /label="lac_promoter" misc_feature 6284..6302 /label="M13r_primer" misc_feature 6316..6366 /label="decoy" ORIGIN 1 ATGAAATAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA TAATTTTCCC 61 GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG ATTATTCTGT 121 AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC ATAGTTCTTA 181 AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA GTTCTAAAAT 241 GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT GCTATTTGAA 301 TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC ACAGATTAAT 361 TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT GTGTGTGATT 421 GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA AGTTTCGTTA 481 CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC AAAAAATTCA 541 GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC AAACTGACTG 601 TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA AGAAGGTCAA 661 AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA GTGCACTGAA 721 ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG TCAACCAACG 781 TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT TTTCTTTATC 841 TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT CTTCACTAGC 901 GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG AATTGAAAGA 961 CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA ACAGAGAAAA 1021 AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA CTTGGTACGT 1081 GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA GTCAAGTCGA 1141 GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC AGTATAATTT 1201 ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA TTTTTCAAGC 1261 ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT GGTTAAATGA 1321 ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG ATCTTCTTCG 1381 CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG GAATATAATG 1441 GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA ACTATTAAAT 1501 TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT TCAAAATCCC 1561 TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT GATTTGGAAA 1621 ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT TCCCAGTTAC 1681 ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA ATTTCTCGGC 1741 GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC TAAACACAAG 1801 TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA AATAGAAGAG 1861 CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG GGATTCCGTT 1921 TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC TGTTGCTCCC 1981 CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA GCCTACCGGA 2041 GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT CTGGTTTTTG 2101 ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT TCTCTCATCT 2161 TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC TCCAAAAACT 2221 TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT TCTTTTTCCT 2281 ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA CCAGCTTCTT 2341 CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG ACCACTAGAT 2401 CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg aatgatacta 2461 acataacata gaacattttc agGAGGACCC TTGGAGGGTA ccggtcgcca ccATGAGGTC 2521 TTCCAAGAAT GTTATCAAGG AGTTCATGAG GTTTAAGGTT CGCATGGAAG GAACGGTCAA 2581 TGGGCACGAG TTTGAAATAG AAGGCGAAGG AGAGGGGAGG CCATACGAAG GCCACAATAC 2641 CGTAAAGCTT AAGGTAACCA AGGGGGGACC TTTGCCATTT GCTTGGGATA TTTTGTCACC 2701 ACAATTTCAG TATGGAAGCA AGGTATATGT CAAGCACCCT GCCGACATAC CAGACTATAA 2761 AAAGCTGTCA TTTCCTGAAG GATTTgtaag tttaaacggg gtgttactaa ctaacaaata 2821 acatttaaat tttcagAAAT GGGAAAGGGT CATGAACTTT GAAGACGGTG GCGTCGCTAC 2881 TGTCACCCAA GATTCCAGCT TGCAAGATGG CTGTTTCATC TACAAGGTCA AGTTCATTGG 2941 CGTGAACTTT CCTTCCGATG GACCTGTTAT GCAAAAGAAG ACAATGGGCT GGGAAGCCAG 3001 CACTGAGCGT TTGTATCCTC GTGATGGCGT GTTGAAAGGA GAGATTCATA AGGCTCTGAA 3061 GCTGAAAGAC GGgtaagttt aaactatatc ttactaacta actgtagaga tttaaatttt 3121 cagTGGTCAT TACCTAGTTG AATTCAAAAG TATTTACATG GCAAAGAAGC CTGTGCAGCT 3181 ACCAGGGTAC TACTATGTTG ACTCCAAACT GGATATAACA AGCCACAACG AAGACTATAC 3241 AATCGTTGAG CAGTATGAAA GAACCGAGGG ACGCCACCAT CTGTTCCTTT AGcggccgcg 3301 actctagaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa 3361 taataggcct aCTAGCCGGC CATACAAGTA ATCCGGATGA TCGACGCCaA CGTCGTTGAA 3421 TTTTCAAATT TTAAATACTG AATATTTGTT TTTTTTCCTA TTATTTATTT ATTCTCTTTG 3481 TGTTTTTTTT CTTGCTTTCT AAAAAATTAA TTCAATCCAA ATCTAAacat ttttttttct 3541 ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt 3601 tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac 3661 aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat 3721 atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa 3781 aaataagcaa agaaattagg cgaacgatgA GAATTGTCCT CGCTTGGGCC CTTTCGTCTC 3841 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA 3901 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT 3961 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 4021 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT 4081 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 4141 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 4201 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 4261 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 4321 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 4381 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 4441 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 4501 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 4561 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 4621 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 4681 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 4741 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 4801 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 4861 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 4921 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 4981 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 5041 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 5101 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 5161 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 5221 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 5281 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 5341 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 5401 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 5461 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 5521 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 5581 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 5641 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA 5701 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 5761 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 5821 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 5881 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 5941 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 6001 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 6061 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 6121 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 6181 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 6241 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA 6301 TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat 6361 tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA // 1715 LOCUS ADDGENE_1715 6420 bp DNA circular SYN 12/31/1969 DEFINITION pPD158.114 complete plasmid sequence KEYWORDS . SOURCE pPD158.114 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1715 at www.addgene.org FEATURES Location/Qualifiers source 1..6420 /organism="Addgene plasmid pPD158.114" /mol_type="other DNA" misc_feature 19..42 /label="myo3.-2341" misc_feature 2338..2361 /label="myo3.Tsa" misc_feature 2434..2475 /label="SynIVS.A" misc_feature 2506..2532 /label="RFP.N" CDS 2506..3285 /label="Orf0" /translation="MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFVSLNGVLLTNK *HLNFQKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGW EASTERLYPRDGVLKGEIHKALKLKDG*V*TISY*LTVEI*IFSGHYLVEFKSIYMAK KPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRHHLFL" misc_feature 2779..2829 /label="RFPivs.2" misc_feature 2857..2886 /label="RFP.V105A" misc_feature 3066..3116 /label="RFP.ivs4" misc_feature 3184..3213 /label="RFP.S197T" misc_feature 3255..3282 /label="RFP.C" misc_feature 3490..3519 /label="let858.3'" promoter 4200..4228 /label="Amp_promoter" CDS 4270..5130 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4270..5130 /label="Ampicillin" /gene="Ampicillin" rep_origin 5285..5904 /label="pBR322_origin" misc_feature 6120..6146 /label="lacI.C" promoter 6213..6242 /label="lac_promoter" misc_feature 6277..6295 /label="M13r_primer" misc_feature 6309..6359 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGGTCGG CTATAATAAG TTCTTGAATA AAATAATTTT CCCGACAAAA 61 CATGAGTATT TCTTTCGAAA ATAAAAGTGC AGGCTAATTA GAGATTATTC TGTAATTAAC 121 TGCATAATTT GTCACGTGCC ATAGTTTTAC ATTCCACTAC GTCATAGTTC TTAAAATACT 181 AATCTCCTGA AAATAGAAGT AGGTGAAGAA AGTTTAATTA TCAGTTCTAA AATGACAATT 241 GATCTTTGGA ATATGTTCTG AAACTACCGA TCATTGAACA GATGCTATTT GAATGATATA 301 GAATTGTATA TTTGCAATTT CTGAAACGCG TTCTTAAAGG CACACAGATT AATTCAAAAG 361 GGTCTGGCCG CAAAAAGGTT TATGGTGGCC GATTTTGAGT TTTGTGTGTG ATTGCTTTTT 421 CACAATCAGT GTTTTCAGGA TTATGTGATG AACTAGATCT TCAAGTTTCG TTACATTTCA 481 TATGTTTTCG GAACTCACGA AGTACATATT GGGTATTGTG CTCAAAAAAT TCAGCAATCA 541 GCTTCGCTCC GCTGACTTTA GAACCCAAAA AAATAGTATG GCCAAACTGA CTGTGTTACG 601 ATCATTTCAA TTTTTCAATA CATATTTAAG ATTTCTAAGA GTAAGAAGGT CAAAAACTGT 661 TCTGGAATAC ATATATATTT TTCAGGTTAC AATTAGTCAA AAAGTGCACT GAAATATACG 721 TTTTAATTTC ACGAATAACC CAATTAGTTC AATGTATTTT TGGTCAACCA ACGTTAAAGT 781 TTGGCTTCCA ACCAATTATC ATTTCTGATC AACCACAATG TTTTTTCTTT ATCTGCAAGT 841 TAATTTTTTA TTTTTATCCA GATGTTTGGC ATATTTTTCA ATTCTTCACT AGCGCCCACT 901 TCTTGCACTT CCGGCGCCCT GAATCTAATG CATCTGTTGC AAGAATTGAA AGACCAATCA 961 ACACATTGTT TTCTTCACGA GATACTGAAG AAAATGAATA AAAACAGAGA AAAAGAGCCA 1021 TGTGATTAGT GACAACTGTT GCTAACAGAT ACCATAGCTT GGACTTGGTA CGTGATGGCA 1081 ACGTATGGGT CAACAAAAAT GATTGCAGAG GGGGTGCAAA ACAGTCAAGT CGAGAAAATA 1141 TGAAAAACAG AAAACAAAGA ACAGAAAAAT GGGTTTGAGA GTCAGTATAA TTTATAAAAG 1201 AAAAATTGTA CATAGAAATT AACCATTTTT GTAGAAGAAG TTATTTTTCA AGCATCGTTA 1261 AAAATTATTC AAAGCACCTT ATTTCATATT TAATTTTAAA CATGGTTAAA TGAACAACAC 1321 GGTGCGCAAT CAGGAAAACT TGAAATCTGA AACTGTTGTT GTGATCTTCT TCGCAACTGT 1381 TCAGATAGCA CTAGTGTAAT GTTAAGAGTG CGCGAATATA ATGGAATATA ATGGATCACA 1441 CCTCCTGCCA TCAGGTAAAC GTCTCTGTTA TCACATATTT CCAACTATTA AATTTTTACC 1501 TTTTACAGTT TTACATTTTT TTGAAAAAAG TAACTTTTTG TCTTCAAAAT CCCTGACGAA 1561 AATATCAAAT ATTTTAATCG AGACTGCAGA GGAACCGATT GATGATTTGG AAAATCCAGC 1621 TTTACCTGTG TAAGAACTGA AAAGTTTCAT AACCCTAGGG TATTCCCAGT TACATTCCCC 1681 ACTGGCTAAC AATAGCACCC AGTTTTTCAT CACCTTTCTT CAAATTTCTC GGCGATTTGT 1741 TAAAAACAAA ATTTGTGTCC CTTCTCTGAT ATCTCTATGT CTCTAAACAC AAGTTCATCG 1801 GAAAACGAAG GAGGGTAGGT GTTGGTTGGG CTCCCGAAGT GAAAATAGAA GAGCAAGAAT 1861 AGAATATTAG AGAGAGAGTG CAGAGAGGGC GGGATAGCTC CCGGGATTCC GTTTTCTTCT 1921 TCTTTATCTT CAACGATGAT GTGTGTGCGT GTTGTATAGA TTCTGTTGCT CCCCCACAAC 1981 TCGCTCCGAA GGCTCAATAC AATTCAATTG ATATTGGAGG AGAGCCTACC GGAGTGGGAG 2041 GATAAGAAGA AACATAAGAA GAAGAAGAAG AAGAAGCATG CTTCTGGTTT TTGATGCTAT 2101 GAAAACGGCA CAAAAAGATG ATTGAGGTCC CTTTTCAATA CCTTCTCTCA TCTTTCAAAT 2161 CCCATTGAAA CCTAAAACTT CTCACCACGC TTTACCATTG TTCTCCAAAA ACTTATAGCA 2221 ATGTCTATAA CTTTTTTATC TCTGAAAAGC AGTGTTCCAT TTTTCTTTTT CCTATTTTAT 2281 TTCAATTGTT TCTCACATTT CGTTTGGATT CTTTGCTTGT CAACCAGCTT CTTCTTCCAC 2341 TTTTACCGTC TAATTTTCAG GGCAGGGAGC CATCAAACCC ACGACCACTA GATCCATCTA 2401 GAGGATCCCC GGGATTGGCC AAAGGACCCA AAGgtatgtt tcgaatgata ctaacataac 2461 atagaacatt ttcagGAGGA CCCTTGGAGG GTAccggtcg ccaccATGAG GTCTTCCAAG 2521 AATGTTATCA AGGAGTTCAT GAGGTTTAAG GTTCGCATGG AAGGAACGGT CAATGGGCAC 2581 GAGTTTGAAA TAGAAGGCGA AGGAGAGGGG AGGCCATACG AAGGCCACAA TACCGTAAAG 2641 CTTAAGGTAA CCAAGGGGGG ACCTTTGCCA TTTGCTTGGG ATATTTTGTC ACCACAATTT 2701 CAGTATGGAA GCAAGGTATA TGTCAAGCAC CCTGCCGACA TACCAGACTA TAAAAAGCTG 2761 TCATTTCCTG AAGGATTTgt aagtttaaac ggggtgttac taactaacaa ataacattta 2821 aattttcagA AATGGGAAAG GGTCATGAAC TTTGAAGACG GTGGCGTCGC TACTGTCACC 2881 CAAGATTCCA GCTTGCAAGA TGGCTGTTTC ATCTACAAGG TCAAGTTCAT TGGCGTGAAC 2941 TTTCCTTCCG ATGGACCTGT TATGCAAAAG AAGACAATGG GCTGGGAAGC CAGCACTGAG 3001 CGTTTGTATC CTCGTGATGG CGTGTTGAAA GGAGAGATTC ATAAGGCTCT GAAGCTGAAA 3061 GACGGgtaag tttaaactat atcttactaa ctaactgtag agatttaaat tttcagTGGT 3121 CATTACCTAG TTGAATTCAA AAGTATTTAC ATGGCAAAGA AGCCTGTGCA GCTACCAGGG 3181 TACTACTATG TTGATACCAA ACTGGATATC ACAAGCCACA ACGAAGACTA TACAATCGTT 3241 GAGCAGTATG AAAGAACCGA GGGACGCCAC CATCTGTTCC TTTAGcggcc gcgactctag 3301 aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg 3361 cctaCTAGCC GGCCATACAA GTAATCCGGA TGATCGACGC CaACGTCGTT GAATTTTCAA 3421 ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT 3481 TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg 3541 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc 3601 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga 3661 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg 3721 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag 3781 caaagaaatt aggcgaacga tgAGAATTGT CCTCGCTTGG GCCCTTTCGT CTCGCGCGTT 3841 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 3901 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 3961 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 4021 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC 4081 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 4141 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 4201 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 4261 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 4321 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 4381 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 4441 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 4501 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 4561 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 4621 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 4681 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 4741 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 4801 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 4861 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 4921 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 4981 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 5041 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 5101 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 5161 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 5221 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 5281 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 5341 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 5401 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 5461 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 5521 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 5581 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 5641 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 5701 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 5761 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 5821 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 5881 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 5941 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 6001 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 6061 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 6121 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 6181 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 6241 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC 6301 GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA 6361 GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1716 LOCUS ADDGENE_1716 5428 bp DNA circular SYN 12/31/1969 DEFINITION pPD176.86 complete plasmid sequence KEYWORDS . SOURCE pPD176.86 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1716 at www.addgene.org FEATURES Location/Qualifiers source 1..5428 /organism="Addgene plasmid pPD176.86" /mol_type="other DNA" misc_feature 77..118 /label="SynIVS.A" misc_feature 138..163 /label="pes10.UAS-270" misc_feature 385..407 /label="pes10.Tsa" promoter complement(453..471) /label="T7_promoter" CDS 488..2602 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFLQPGGSMVKPVTLYDVAEYAGVSYQTVSRVVNQASHVS AKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQ LGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALF LDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYL TRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR VGADVSLNTLILTNCYYLNFQISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRL LQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRSSLRVSL NDELLTNLPNLNFQPPKKKRKV" gene 488..1336 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 659..709 /label="SynIVS.R" misc_feature 731..750 /label="gfp.S65C" misc_feature 864..914 /label="SynIVS.S" misc_feature 1079..1129 /label="SynIVS.T" misc_feature 1397..1423 /label="lacI.N" misc_feature 2198..2248 /label="lacI.IVS1" misc_feature 2486..2512 /label="lacI.C" misc_feature 2525..2575 /label="lacI.IVS2" misc_feature 2783..2812 /label="let858.3'" promoter 3208..3236 /label="Amp_promoter" gene 3278..4138 /label="Ampicillin" /gene="Ampicillin" CDS 3278..4138 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4293..4912 /label="pBR322_origin" misc_feature 5128..5154 /label="lacI.C" promoter 5221..5250 /label="lac_promoter" misc_feature 5285..5303 /label="M13r_primer" misc_feature 5317..5367 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCggatc cccgggattg 61 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga 121 ggacccttgg agggtaccta atttgatcga taatatgtac atattttgtt attcgttccg 181 gtatcgtacc tccacctacc ataaatccca taccctacat ctttATCGAT TTTTTGCAAA 241 TTACGAGCGT TGTAGGGGGC GGAgCGATAG GTCCTATAGG TTTTgGTATA TCATCATTCA 301 TTCATTCATT GGTACATTCA TTTACCCACC TTCCTCTTTC TGAGCTTCTC TGGAGTTCTG 361 TGCTTCCTTT TTCCCTTATC TTTATACTGT AATTTTTAAC TTTCAGgcat tgattggatc 421 cTATCGATTC GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT 481 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 541 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 601 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 661 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 721 CACTACTTTC TgTTATGGTG TTCAATGCTT cTCGAGATAC CCAGATCATA TGAAACAGCA 781 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 841 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 901 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 961 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 1021 CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt 1081 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 1141 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 1201 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT 1261 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 1321 TGGGATTACA CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCCTGCA 1381 GCCCGGGGGA TCCatgGtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt 1441 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg 1501 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca 1561 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc 1621 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 1681 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 1741 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc 1801 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc 1861 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt 1921 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg 1981 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga 2041 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 2101 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg 2161 cgccattacc gagtccgggc tgcgcgttgg tgcggatGTA AGTTTAAACA CCTTAATACT 2221 AACTAACTGT TATTATTTAA ATTTTCAGat ctcggtagtg ggatacgacg ataccgaaga 2281 cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca 2341 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 2401 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 2461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gaAGCAGCCT 2521 GAGGgtaagt ttaaacgatg aattactaac taacttgcca aatttaaatt ttcagCCTCC 2581 TAAGAAGAAG AGGAAGGTTT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG 2641 CACCCCAGGC TTTACACTTT ATGCTTCCGA CCTGCAgcCC GGATGATCGA CGCCaACGTC 2701 GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC 2761 TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt 2821 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag 2881 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa 2941 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc 3001 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact 3061 agtaaaaaat aagcaaagaa attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCAAA 3121 AGGCCTTTTT TTTTTGGCGC CGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 3181 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 3241 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 3301 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 3361 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 3421 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 3481 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 3541 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 3601 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 3661 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 3721 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 3781 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 3841 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 3901 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 3961 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 4021 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 4081 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 4141 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 4201 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 4261 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4321 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4381 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4441 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4501 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4561 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4621 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4681 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4741 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4801 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4861 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4921 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 4981 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 5041 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 5101 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 5161 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 5221 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 5281 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 5341 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 5401 GGATACGCTA ACAACTTGGA AATGAAAT // 1717 LOCUS ADDGENE_1717 5479 bp DNA circular SYN 12/31/1969 DEFINITION pPD177.66 complete plasmid sequence KEYWORDS . SOURCE pPD177.66 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1717 at www.addgene.org FEATURES Location/Qualifiers source 1..5479 /organism="Addgene plasmid pPD177.66" /mol_type="other DNA" misc_feature 77..118 /label="SynIVS.A" misc_feature 138..163 /label="pes10.UAS-270" misc_feature 385..407 /label="pes10.Tsa" promoter complement(453..471) /label="T7_promoter" gene 488..1348 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 488..1357 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFGYGLQCFARYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 659..709 /label="SynIVS.R" misc_feature 728..757 /label="gfp.GLA" misc_feature 864..914 /label="SynIVS.S" misc_feature 1079..1129 /label="SynIVS.T" misc_feature 1230..1261 /label="gfp.T203Y" misc_feature 1371..1421 /label="SynIVS.M" CDS 1445..2653 /label="Orf1" /translation="MVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMA ELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSI IFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAERE GDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADVSLNTLIL TNCYYLNFQISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGN QLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRSSLRVSLNDELLTNLPNLN FQPPKKKRKV" misc_feature 1448..1474 /label="lacI.N" misc_feature 2249..2299 /label="lacI.IVS1" misc_feature 2537..2563 /label="lacI.C" misc_feature 2576..2626 /label="lacI.IVS2" misc_feature 2834..2863 /label="let858.3'" promoter 3259..3287 /label="Amp_promoter" CDS 3329..4189 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3329..4189 /label="Ampicillin" /gene="Ampicillin" rep_origin 4344..4963 /label="pBR322_origin" misc_feature 5179..5205 /label="lacI.C" promoter 5272..5301 /label="lac_promoter" misc_feature 5336..5354 /label="M13r_primer" misc_feature 5368..5418 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCggatc cccgggattg 61 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga 121 ggacccttgg agggtaccta atttgatcga taatatgtac atattttgtt attcgttccg 181 gtatcgtacc tccacctacc ataaatccca taccctacat ctttATCGAT TTTTTGCAAA 241 TTACGAGCGT TGTAGGGGGC GGAgCGATAG GTCCTATAGG TTTTgGTATA TCATCATTCA 301 TTCATTCATT GGTACATTCA TTTACCCACC TTCCTCTTTC TGAGCTTCTC TGGAGTTCTG 361 TGCTTCCTTT TTCCCTTATC TTTATACTGT AATTTTTAAC TTTCAGgcat tgattggatc 421 cTATCGATTC GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT 481 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 541 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 601 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 661 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 721 CACTACTTTC GGTTATGGTC TTCAATGCTT CGCCAGATAC CCAGATCATA TGAAACAGCA 781 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 841 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 901 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 961 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 1021 CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt 1081 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 1141 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 1201 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCTACC AATCTGCCCT 1261 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 1321 TGGGATTACA CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT gtaagtttaa 1381 actttctcat actaactaac gcgctctatt taaattttca gAATTCCTGC AGCCCGGGGG 1441 ATCCatgGtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca 1501 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt 1561 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg 1621 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca 1681 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat 1741 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg 1801 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc 1861 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag 1921 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg 1981 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct 2041 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg 2101 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt 2161 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac 2221 cgagtccggg ctgcgcgttg gtgcggatGT AAGTTTAAAC ACCTTAATAC TAACTAACTG 2281 TTATTATTTA AATTTTCAGa tctcggtagt gggatacgac gataccgaag acagctcatg 2341 ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 2401 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 2461 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 2521 gttggccgat tcattaatgc agctggcacg acaggtttcc cgaAGCAGCC TGAGGgtaag 2581 tttaaacgat gaattactaa ctaacttgcc aaatttaaat tttcagCCTC CTAAGAAGAA 2641 GAGGAAGGTT TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG GCACCCCAGG 2701 CTTTACACTT TATGCTTCCG ACCTGCAgcC CGGATGATCG ACGCCaACGT CGTTGAATTT 2761 TCAAATTTTA AATACTGAAT ATTTGTTTTT TTTCCTATTA TTTATTTATT CTCTTTGTGT 2821 TTTTTTTCTT GCTTTCTAAA AAATTAATTC AATCCAAATC TAAacatttt tttttctctt 2881 tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat 2941 cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa 3001 aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg 3061 gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa 3121 taagcaaaga aattaggcga acgatgAGAA TTGTCCTCGC TTGGGCCCAA AAGGCCTTTT 3181 TTTTTTGGCG CCGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 3241 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 3301 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 3361 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 3421 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 3481 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 3541 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 3601 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 3661 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 3721 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 3781 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 3841 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 3901 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 3961 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 4021 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 4081 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 4141 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 4201 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 4261 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 4321 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 4381 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 4441 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 4501 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 4561 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 4621 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 4681 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 4741 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 4801 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 4861 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 4921 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 4981 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 5041 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 5101 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 5161 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 5221 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 5281 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 5341 CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact aactaactat 5401 tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA TGGATACGCT 5461 AACAACTTGG AAATGAAAT // 1718 LOCUS ADDGENE_1718 5428 bp DNA circular SYN 12/31/1969 DEFINITION pPD177.71 complete plasmid sequence KEYWORDS . SOURCE pPD177.71 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1718 at www.addgene.org FEATURES Location/Qualifiers source 1..5428 /organism="Addgene plasmid pPD177.71" /mol_type="other DNA" misc_feature 77..118 /label="SynIVS.A" misc_feature 138..163 /label="pes10.UAS-270" misc_feature 385..407 /label="pes10.Tsa" promoter complement(453..471) /label="T7_promoter" CDS 488..2602 /label="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSWGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFLQPGGSMVKPVTLYDVAEYAGVSYQTVSRVVNQASHVS AKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQ LGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALF LDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYL TRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR VGADVSLNTLILTNCYYLNFQISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRL LQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRSSLRVSL NDELLTNLPNLNFQPPKKKRKV" misc_feature 659..709 /label="SynIVS.R" misc_feature 734..758 /label="gfp.Y66W" misc_feature 864..914 /label="SynIVS.S" misc_feature 1018..1042 /label="gfp.N146I" misc_feature 1037..1060 /label="gfp.M153T" misc_feature 1057..1078 /label="gfp.V163A" misc_feature 1079..1129 /label="SynIVS.T" misc_feature 1397..1423 /label="lacI.N" misc_feature 2198..2248 /label="lacI.IVS1" misc_feature 2486..2512 /label="lacI.C" misc_feature 2525..2575 /label="lacI.IVS2" misc_feature 2783..2812 /label="let858.3'" promoter 3208..3236 /label="Amp_promoter" gene 3278..4138 /label="Ampicillin" /gene="Ampicillin" CDS 3278..4138 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4293..4912 /label="pBR322_origin" misc_feature 5128..5154 /label="lacI.C" promoter 5221..5250 /label="lac_promoter" misc_feature 5285..5303 /label="M13r_primer" misc_feature 5317..5367 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCggatc cccgggattg 61 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga 121 ggacccttgg agggtaccta atttgatcga taatatgtac atattttgtt attcgttccg 181 gtatcgtacc tccacctacc ataaatccca taccctacat ctttATCGAT TTTTTGCAAA 241 TTACGAGCGT TGTAGGGGGC GGAgCGATAG GTCCTATAGG TTTTgGTATA TCATCATTCA 301 TTCATTCATT GGTACATTCA TTTACCCACC TTCCTCTTTC TGAGCTTCTC TGGAGTTCTG 361 TGCTTCCTTT TTCCCTTATC TTTATACTGT AATTTTTAAC TTTCAGgcat tgattggatc 421 cTATCGATTC GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT 481 AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 541 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 601 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt 661 aagtttaaac atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT 721 CACTACTTTC TCATGGGGCG TTCAATGCTT CTCGAGATAC CCAGATCATA TGAAACAGCA 781 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 841 AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat 901 atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA 961 GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA 1021 CTATATTTCA CACAACGTAT ACATCACTGC CGACAAACAA AAGAATGGGA TCAAAGCGgt 1081 aagtttaaac atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT 1141 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC 1201 AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT 1261 TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC 1321 TGGGATTACA CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCCTGCA 1381 GCCCGGGGGA TCCatgGtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt 1441 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg 1501 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca 1561 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc 1621 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 1681 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 1741 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc 1801 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc 1861 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt 1921 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg 1981 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga 2041 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 2101 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg 2161 cgccattacc gagtccgggc tgcgcgttgg tgcggatGTA AGTTTAAACA CCTTAATACT 2221 AACTAACTGT TATTATTTAA ATTTTCAGat ctcggtagtg ggatacgacg ataccgaaga 2281 cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca 2341 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 2401 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 2461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gaAGCAGCCT 2521 GAGGgtaagt ttaaacgatg aattactaac taacttgcca aatttaaatt ttcagCCTCC 2581 TAAGAAGAAG AGGAAGGTTT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG 2641 CACCCCAGGC TTTACACTTT ATGCTTCCGA CCTGCAgcCC GGATGATCGA CGCCaACGTC 2701 GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC 2761 TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt 2821 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag 2881 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa 2941 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc 3001 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact 3061 agtaaaaaat aagcaaagaa attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCAAA 3121 AGGCCTTTTT TTTTTGGCGC CGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 3181 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 3241 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 3301 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 3361 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 3421 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 3481 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 3541 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 3601 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 3661 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 3721 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 3781 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 3841 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 3901 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 3961 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 4021 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 4081 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 4141 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 4201 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 4261 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4321 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4381 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4441 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4501 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4561 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4621 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4681 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4741 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4801 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4861 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4921 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 4981 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 5041 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 5101 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 5161 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 5221 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 5281 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 5341 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 5401 GGATACGCTA ACAACTTGGA AATGAAAT // 1719 LOCUS ADDGENE_1719 6371 bp DNA circular SYN 12/31/1969 DEFINITION pPD172.01 complete plasmid sequence KEYWORDS . SOURCE pPD172.01 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1719 at www.addgene.org FEATURES Location/Qualifiers source 1..6371 /organism="Addgene plasmid pPD172.01" /mol_type="other DNA" misc_feature 16..51 /label="sur5.-1.0k" misc_feature 1027..1058 /label="sur5.tsa" promoter complement(1111..1129) /label="T7_promoter" CDS 1146..3260 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFLQPGGSMVKPVTLYDVAEYAGVSYQTVSRVVNQASHVS AKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQ LGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALF LDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYL TRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR VGADVSLNTLILTNCYYLNFQISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRL LQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRSSLRVSL NDELLTNLPNLNFQPPKKKRKV" gene 1146..1994 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1317..1367 /label="SynIVS.R" misc_feature 1389..1408 /label="gfp.S65C" misc_feature 1522..1572 /label="SynIVS.S" misc_feature 1737..1787 /label="SynIVS.T" misc_feature 2055..2081 /label="lacI.N" misc_feature 2856..2906 /label="lacI.IVS1" misc_feature 3144..3170 /label="lacI.C" misc_feature 3183..3233 /label="lacI.IVS2" misc_feature 3441..3470 /label="let858.3'" promoter 4151..4179 /label="Amp_promoter" gene 4221..5081 /label="Ampicillin" /gene="Ampicillin" CDS 4221..5081 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5236..5855 /label="pBR322_origin" misc_feature 6071..6097 /label="lacI.C" promoter 6164..6193 /label="lac_promoter" misc_feature 6228..6246 /label="M13r_primer" misc_feature 6260..6310 /label="decoy" ORIGIN 1 AAGCTTGGGC TGCAGAGTGG AGAGTGTAGT CCCTCGTCGG ctctctgtgt atttctttca 61 atgcctgtgt ctcggtctat ctttgtctct atctgtttta aaagtttttt gcaaagtttc 121 aatttctttt ttattttctc ttcttttttt gcgcatttat tttttttaaa gagcgctgtt 181 cttcaattgg aaaacagtat aacgatcgaa ataaaaaaaa aacttgaaat agtatcggaa 241 ttgagatggg ggcaattgaa attgaagacg caacaaaaac atgtttttat cgtctgaaat 301 tatctaaact ttgaatctaa cgtgtcaatg gctactttta cagtagactt tttagagtaa 361 tgttaaaaag gataatatag gtttttcttc aaactatttt gtcacgccgc atatattctt 421 ttcatattcc attcgcaaag tgcatgatgt aacaaaaatg aaacgtcata attttttaat 481 ctttcacact ttttttgccg gccgttaaat caaaatcaaa tcaacttaat ccaacacgtg 541 cgggcttgca attttattac accggtgtcc tacagcgcca tgttatcttc tttgattaga 601 gatctctcac gcaacatttg acattgaaaa ttttgtctcc gaattgttgt gtacctactt 661 tgtcccgatg tcccaaattt tcactgataa caagagatat ctcaactgtt cttatcacgt 721 tgatacgtag aacgttttac actaaaccaa attgcagtcc ccttctacga accgtttgct 781 aattgttttg catagaacag tgatcaatgt gttgtgctca ctagtcaatg atgttcgtct 841 ctcatttttc ctacaacttc actgaaaagg ttttgaatat ctggtgattg taggcaatca 901 caccacagtt aatggcctgg caaataatga taagcgtgtg ataaacctca caaaaatacg 961 cctagaagtg gtgatatcgc tagttttgtg tcctttcgat ttttttcgtg ttgctttttt 1021 ctgtggataa acctttgaac tTTACATTTT GTTTTCAGAA TAACCGCTCT AGAGGATCCT 1081 ATCGATTCGC GGCCGCTGTA CACCCGGGTG CCCTATAGTG AGTCGTATTG GTACCGGTAG 1141 AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 1201 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT 1261 ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGgtaa 1321 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA ACACTTGTCA 1381 CTACTTTCTg TTATGGTGTT CAATGCTTcT CGAGATACCC AGATCATATG AAACAGCATG 1441 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG 1501 ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa ccatacatat 1561 ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 1621 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 1681 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTgtaa 1741 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC TTCAAAATTA 1801 GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA 1861 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT 1921 CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG 1981 GGATTACACA TGGCATGGAc GAACTATACA AAagcccaca AGCTTTGGAA TTCCTGCAGC 2041 CCGGGGGATC CatgGtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 2101 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 2161 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 2221 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 2281 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 2341 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 2401 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 2461 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 2521 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 2581 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 2641 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 2701 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 2761 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 2821 ccattaccga gtccgggctg cgcgttggtg cggatGTAAG TTTAAACACC TTAATACTAA 2881 CTAACTGTTA TTATTTAAAT TTTCAGatct cggtagtggg atacgacgat accgaagaca 2941 gctcatgtta tatcccgccg tcaaccacca tcaaacagga ttttcgcctg ctggggcaaa 3001 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 3061 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 3121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga AGCAGCCTGA 3181 GGgtaagttt aaacgatgaa ttactaacta acttgccaaa tttaaatttt cagCCTCCTA 3241 AGAAGAAGAG GAAGGTTTGA GCGCAACGCA ATTAATGTAA GTTAGCTCAC TCATTAGGCA 3301 CCCCAGGCTT TACACTTTAT GCTTCCGACC TGCAgcCCGG ATGATCGACG CCaACGTCGT 3361 TGAATTTTCA AATTTTAAAT ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC 3421 TTTGTGTTTT TTTTCTTGCT TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt 3481 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt 3541 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac 3601 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag 3661 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag 3721 taaaaaataa gcaaagaaat taggcgaacg atgAGAATTG TCCTCGCTTG GGCCCTTTCG 3781 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 3841 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 3901 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 3961 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 4021 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 4081 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 4141 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 4201 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 4261 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 4321 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 4381 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 4441 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 4501 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 4561 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 4621 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 4681 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 4741 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 4801 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 4861 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 4921 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 4981 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 5041 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 5101 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 5161 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 5221 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 5281 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 5341 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 5401 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 5461 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 5521 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 5581 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 5641 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 5701 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 5761 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 5821 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 5881 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 5941 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 6001 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 6061 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 6121 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 6181 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 6241 ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact attctcattt 6301 aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG CTAACAACTT 6361 GGAAATGAAA T // 1720 LOCUS ADDGENE_1720 5343 bp DNA circular SYN 12/31/1969 DEFINITION pPD176.36 complete plasmid sequence KEYWORDS . SOURCE pPD176.36 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1720 at www.addgene.org FEATURES Location/Qualifiers source 1..5343 /organism="Addgene plasmid pPD176.36" /mol_type="other DNA" promoter complement(83..101) /label="T7_promoter" CDS 118..2232 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKQHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKSPQALEFLQPGGSMVKPVTLYDVAEYAGVSYQTVSRVVNQASHVS AKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQ LGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALF LDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYL TRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR VGADVSLNTLILTNCYYLNFQISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRL LQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRSSLRVSL NDELLTNLPNLNFQPPKKKRKV" gene 118..966 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 289..339 /label="SynIVS.R" misc_feature 361..380 /label="gfp.S65C" misc_feature 494..544 /label="SynIVS.S" misc_feature 709..759 /label="SynIVS.T" misc_feature 1027..1053 /label="lacI.N" misc_feature 1828..1878 /label="lacI.IVS1" misc_feature 2116..2142 /label="lacI.C" misc_feature 2155..2205 /label="lacI.IVS2" misc_feature 2413..2442 /label="let858.3'" promoter 3123..3151 /label="Amp_promoter" CDS 3193..4053 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3193..4053 /label="Ampicillin" /gene="Ampicillin" rep_origin 4208..4827 /label="pBR322_origin" misc_feature 5043..5069 /label="lacI.C" promoter 5136..5165 /label="lac_promoter" misc_feature 5200..5218 /label="M13r_primer" misc_feature 5232..5282 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA CCGCGGTTTT GGCGCGGATC CTATCGATTC 61 GCGGCCGCTG TACACCCGGG TGCCCTATAG TGAGTCGTAT TGGTACCGGT AGAAAAAATG 121 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 181 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 241 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac 301 atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC 361 TgTTATGGTG TTCAATGCTT cTCGAGATAC CCAGATCATA TGAAACAGCA TGACTTTTTC 421 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 481 AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt 541 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT 601 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA 661 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac 721 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC 781 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT 841 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT 901 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA 961 CATGGCATGG AcGAACTATA CAAAagccca caAGCTTTGG AATTCCTGCA GCCCGGGGGA 1021 TCCatgGtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag 1081 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg 1141 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc 1201 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa 1261 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg 1321 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc 1381 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct 1441 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt 1501 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt 1561 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg 1621 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc 1681 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt 1741 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc 1801 gagtccgggc tgcgcgttgg tgcggatGTA AGTTTAAACA CCTTAATACT AACTAACTGT 1861 TATTATTTAA ATTTTCAGat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt 1921 tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg 1981 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc 2041 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg 2101 ttggccgatt cattaatgca gctggcacga caggtttccc gaAGCAGCCT GAGGgtaagt 2161 ttaaacgatg aattactaac taacttgcca aatttaaatt ttcagCCTCC TAAGAAGAAG 2221 AGGAAGGTTT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCCCAGGC 2281 TTTACACTTT ATGCTTCCGA CCTGCAgcCC GGATGATCGA CGCCaACGTC GTTGAATTTT 2341 CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC TCTTTGTGTT 2401 TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt ttttctcttt 2461 ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc 2521 ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa 2581 ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg 2641 aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat 2701 aagcaaagaa attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCTTT CGTCTCGCGC 2761 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 2821 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 2881 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 2941 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 3001 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 3061 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 3121 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 3181 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 3241 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 3301 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 3361 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 3421 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 3481 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 3541 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 3601 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 3661 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 3721 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 3781 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 3841 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 3901 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 3961 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 4021 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 4081 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 4141 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 4201 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 4261 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 4321 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 4381 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 4441 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 4501 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 4561 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 4621 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 4681 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 4741 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 4801 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 4861 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 4921 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 4981 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 5041 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 5101 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 5161 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 5221 TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc 5281 agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA 5341 AAT // 1721 LOCUS ADDGENE_1721 6581 bp DNA circular SYN 12/31/1969 DEFINITION pPD138.11 complete plasmid sequence KEYWORDS . SOURCE pPD138.11 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1721 at www.addgene.org FEATURES Location/Qualifiers source 1..6581 /organism="Addgene plasmid pPD138.11" /mol_type="other DNA" CDS 22..780 /label="EGFP" /gene="EGFP" /note="Orf0" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSRAQASNSN" gene 22..738 /label="EGFP" /gene="EGFP" misc_feature 25..51 /label="EGFP.N" misc_feature 718..738 /label="EGFP.C" CDS complement(7..840) /label="Orf0" /translation="MTAAPIIFDTRQVGNGSDRRSVGIRSLSSRSESGLVQLVHAESD PGGGHELQQDHVIALLVGVFAQGGLGAQVVVVGQQHGAVADGGVLLVVVGELHAAVLD VVADLEVHLDAVLLLVGHDIDVVAVVVVLQLVPQDVAVLLEVDALQLDAVHQGVALEL HLGAGLVVAVVLEEDGALLDVAFGHGGLEEVVLLHVVGVAAEALHAVGQGGHEGGPGH GQLAGGADELQGQLAVGGIALALAGHAELVAVYVAVQLDQDGHHPGEQLLALAHHGGD R" misc_feature 845..895 /label="SynIVS.L" promoter 2023..2051 /label="Amp_promoter" CDS 2093..2953 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2093..2953 /label="Ampicillin" /gene="Ampicillin" rep_origin 3108..3727 /label="pBR322_origin" misc_feature 3943..3969 /label="lacI.C" promoter 4036..4065 /label="lac_promoter" misc_feature 4100..4118 /label="M13r_primer" misc_feature 4145..4168 /label="myo3.-2341" misc_feature 6464..6487 /label="myo3.Tsa" ORIGIN 1 CTAGCGCTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG 61 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG 121 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG 181 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC 241 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 301 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 361 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 421 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC 481 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG 541 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 601 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 661 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 721 ATGGACGAGC TGTACAAGTC CGGACTCAGA TCTCGAGCTC AAGCTTCGAA TTCCAACTGA 781 GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT 841 CAGAgtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT 901 CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC 961 TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT 1021 TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT 1081 AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT 1141 CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT 1201 TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT 1261 TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC 1321 ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT 1381 TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG 1441 GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA 1501 GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG 1561 TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG 1621 TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA 1681 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 1741 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC 1801 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC 1861 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT 1921 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 1981 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 2041 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 2101 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 2161 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 2221 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 2281 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 2341 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 2401 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 2461 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 2521 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 2581 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 2641 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 2701 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 2761 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 2821 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 2881 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 2941 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 3001 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 3061 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3121 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3181 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3241 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3301 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3361 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3421 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3481 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA 3541 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3601 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3661 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3721 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 3781 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC 3841 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC 3901 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG 3961 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA 4021 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG 4081 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TTGGGCTGCA 4141 GGTCGGCTAT AATAAGTTCT TGAATAAAAT AATTTTCCCG ACAAAACATG AGTATTTCTT 4201 TCGAAAATAA AAGTGCAGGC TAATTAGAGA TTATTCTGTA ATTAACTGCA TAATTTGTCA 4261 CGTGCCATAG TTTTACATTC CACTACGTCA TAGTTCTTAA AATACTAATC TCCTGAAAAT 4321 AGAAGTAGGT GAAGAAAGTT TAATTATCAG TTCTAAAATG ACAATTGATC TTTGGAATAT 4381 GTTCTGAAAC TACCGATCAT TGAACAGATG CTATTTGAAT GATATAGAAT TGTATATTTG 4441 CAATTTCTGA AACGCGTTCT TAAAGGCACA CAGATTAATT CAAAAGGGTC TGGCCGCAAA 4501 AAGGTTTATG GTGGCCGATT TTGAGTTTTG TGTGTGATTG CTTTTTCACA ATCAGTGTTT 4561 TCAGGATTAT GTGATGAACT AGATCTTCAA GTTTCGTTAC ATTTCATATG TTTTCGGAAC 4621 TCACGAAGTA CATATTGGGT ATTGTGCTCA AAAAATTCAG CAATCAGCTT CGCTCCGCTG 4681 ACTTTAGAAC CCAAAAAAAT AGTATGGCCA AACTGACTGT GTTACGATCA TTTCAATTTT 4741 TCAATACATA TTTAAGATTT CTAAGAGTAA GAAGGTCAAA AACTGTTCTG GAATACATAT 4801 ATATTTTTCA GGTTACAATT AGTCAAAAAG TGCACTGAAA TATACGTTTT AATTTCACGA 4861 ATAACCCAAT TAGTTCAATG TATTTTTGGT CAACCAACGT TAAAGTTTGG CTTCCAACCA 4921 ATTATCATTT CTGATCAACC ACAATGTTTT TTCTTTATCT GCAAGTTAAT TTTTTATTTT 4981 TATCCAGATG TTTGGCATAT TTTTCAATTC TTCACTAGCG CCCACTTCTT GCACTTCCGG 5041 CGCCCTGAAT CTAATGCATC TGTTGCAAGA ATTGAAAGAC CAATCAACAC ATTGTTTTCT 5101 TCACGAGATA CTGAAGAAAA TGAATAAAAA CAGAGAAAAA GAGCCATGTG ATTAGTGACA 5161 ACTGTTGCTA ACAGATACCA TAGCTTGGAC TTGGTACGTG ATGGCAACGT ATGGGTCAAC 5221 AAAAATGATT GCAGAGGGGG TGCAAAACAG TCAAGTCGAG AAAATATGAA AAACAGAAAA 5281 CAAAGAACAG AAAAATGGGT TTGAGAGTCA GTATAATTTA TAAAAGAAAA ATTGTACATA 5341 GAAATTAACC ATTTTTGTAG AAGAAGTTAT TTTTCAAGCA TCGTTAAAAA TTATTCAAAG 5401 CACCTTATTT CATATTTAAT TTTAAACATG GTTAAATGAA CAACACGGTG CGCAATCAGG 5461 AAAACTTGAA ATCTGAAACT GTTGTTGTGA TCTTCTTCGC AACTGTTCAG ATAGCACTAG 5521 TGTAATGTTA AGAGTGCGCG AATATAATGG AATATAATGG ATCACACCTC CTGCCATCAG 5581 GTAAACGTCT CTGTTATCAC ATATTTCCAA CTATTAAATT TTTACCTTTT ACAGTTTTAC 5641 ATTTTTTTGA AAAAAGTAAC TTTTTGTCTT CAAAATCCCT GACGAAAATA TCAAATATTT 5701 TAATCGAGAC TGCAGAGGAA CCGATTGATG ATTTGGAAAA TCCAGCTTTA CCTGTGTAAG 5761 AACTGAAAAG TTTCATAACC CTAGGGTATT CCCAGTTACA TTCCCCACTG GCTAACAATA 5821 GCACCCAGTT TTTCATCACC TTTCTTCAAA TTTCTCGGCG ATTTGTTAAA AACAAAATTT 5881 GTGTCCCTTC TCTGATATCT CTATGTCTCT AAACACAAGT TCATCGGAAA ACGAAGGAGG 5941 GTAGGTGTTG GTTGGGCTCC CGAAGTGAAA ATAGAAGAGC AAGAATAGAA TATTAGAGAG 6001 AGAGTGCAGA GAGGGCGGGA TAGCTCCCGG GATTCCGTTT TCTTCTTCTT TATCTTCAAC 6061 GATGATGTGT GTGCGTGTTG TATAGATTCT GTTGCTCCCC CACAACTCGC TCCGAAGGCT 6121 CAATACAATT CAATTGATAT TGGAGGAGAG CCTACCGGAG TGGGAGGATA AGAAGAAACA 6181 TAAGAAGAAG AAGAAGAAGA AGCATGCTTC TGGTTTTTGA TGCTATGAAA ACGGCACAAA 6241 AAGATGATTG AGGTCCCTTT TCAATACCTT CTCTCATCTT TCAAATCCCA TTGAAACCTA 6301 AAACTTCTCA CCACGCTTTA CCATTGTTCT CCAAAAACTT ATAGCAATGT CTATAACTTT 6361 TTTATCTCTG AAAAGCAGTG TTCCATTTTT CTTTTTCCTA TTTTATTTCA ATTGTTTCTC 6421 ACATTTCGTT TGGATTCTTT GCTTGTCAAC CAGCTTCTTC TTCCACTTTT ACCGTCTAAT 6481 TTTCAGGGCA GGGAGCCATC AAACCCACGA CCACTAGATC CATCTAGAGG ATCCCCGGGA 6541 TTGGCCAAAG GACCCAAAGG AGGACCCTTG GAGGGTACCG G // 1722 LOCUS ADDGENE_1722 6429 bp DNA circular SYN 12/31/1969 DEFINITION pPD187.49 complete plasmid sequence KEYWORDS . SOURCE pPD187.49 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1722 at www.addgene.org FEATURES Location/Qualifiers source 1..6429 /organism="Addgene plasmid pPD187.49" /mol_type="other DNA" promoter 864..882 /label="T7_promoter" misc_feature 911..947 /label="SPGFP.N" misc_feature 1920..1948 /label="SPGFP.C" promoter complement(1980..1999) /label="Sp6_promoter" rep_origin 2312..2618 /label="f1_origin" CDS 3134..3928 /label="Neomycin" /gene="Neomycin" /note="Orf1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" gene 3137..3925 /label="Neomycin" /gene="Neomycin" misc_feature complement(4268..4286) /label="M13r_primer" promoter complement(4321..4350) /label="lac_promoter" misc_feature complement(4417..4443) /label="lacI.C" rep_origin complement(4659..5278) /label="pBR322_origin" CDS complement(5433..6293) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(5433..6293) /label="Ampicillin" /gene="Ampicillin" promoter complement(6335..6363) /label="Amp_promoter" ORIGIN 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC 901 GGTAGAAAAA ATGTCAAAAG GAGAGGAATT GTTCACCGGC GTCGTTCCAA TCCTCGTTGA 961 ACTCGACGGC GACGTCAACG GGCACAAGTT TTCCGTCTCT GGAGAGGGAG AGGGAGACGC 1021 AACCTACGGC AAATTGACCC TTAAATTCAT TTGCACCACA Ggtaagtttc gatttttgct 1081 atctttttca cgatttttac tctctttttc tactaactaa cctccttttt gaacattttt 1141 tcagGAAAAT TGCCAGTTCC ATGGCCAACA TTGGTCACAA CCTTCTGTTA CGGCGTCCAA 1201 TGCTTCTCGC GTTACCCCGA TCACATGAAA AGGCACGACT TTTTTAAATC CGCAATGCCA 1261 GAAGGATACG TACAAGAACG CACAATTTTC TTCAAAGATG ACGGAAACTA CAAGACAAGg 1321 taagtttttc ccaacttttt cggacttttt gaccgctttt tatactaact aacggacttt 1381 ttcgtccttt ttcagGGCCG AAGTCAAGTT TGAAGGAGAC ACCCTCGTCA ACCGCATCGA 1441 ACTTAAAGGA ATTGACTTCA AAGAAGACGG AAACATTCTC GGACACAAAC TCGAATACAA 1501 CTACAATTCT CACAACGTTT ACATAATGGC CGACAAACAA AAGAACGGAA TCAAGgtaag 1561 tttcgctttt tgatgatttt tggaccattt ttaggaattt ttcgatacta actaactatt 1621 tttgcctgct ttttcagGTC AACTTCAAGA TTCGTCACAA CATTGAGGAC GGAAGCGTCC 1681 AACTTGCTGA CCATTACCAG CAAAACACCC CGATTGGCGA CGGACCCGTC CTCCTTCCGG 1741 ACAACCACTA CCTCTCAACA CAGAGCGCCC TCTCGAAAGA CCCGAACGAG AAGAGgtaag 1801 tttatcgttt ttcgacgatt tttacccatt tttacgccat ttttgatact aactaacgcc 1861 tttttaccga tttttcagGG ACCACATGGT CCTTTTGGAA TTTGTTACGG CCGCAGGCAT 1921 CACCCACGGC ATGGACGAAC TCTACAAATA GCATTCGTAG AATTCGCTAG AGGGCCCTAT 1981 TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG 2041 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 2101 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 2161 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 2221 GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC 2281 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 2341 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 2401 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 2461 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 2521 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 2581 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 2641 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 2701 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 2761 TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 2821 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 2881 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 2941 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 3001 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT 3061 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG 3121 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 3181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 3241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 3301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 3361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 3421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 3481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 3541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 3601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 3661 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 3721 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 3781 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 3841 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 3901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA 3961 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 4021 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG 4081 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 4141 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 4201 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG 4261 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 4321 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 4381 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 4441 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 4501 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 4561 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4621 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4681 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 4741 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 4801 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 4861 CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4921 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 4981 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 5041 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 5101 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 5161 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 5221 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5281 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 5341 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 5401 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 5461 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 5521 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 5581 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 5641 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 5701 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 5761 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 5821 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 5881 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 5941 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 6001 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 6061 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 6121 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 6181 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 6241 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 6301 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 6361 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 6421 CCTGACGTC // 1723 LOCUS ADDGENE_1723 10665 bp DNA circular SYN 12/31/1969 DEFINITION pPD187.24 complete plasmid sequence KEYWORDS . SOURCE pPD187.24 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1723 at www.addgene.org FEATURES Location/Qualifiers source 1..10665 /organism="Addgene plasmid pPD187.24" /mol_type="other DNA" misc_feature 3462..3490 /label="pie1.tsa" misc_feature 3545..3581 /label="SPGFP.N" misc_feature 4554..4582 /label="SPGFP.C" misc_feature 4627..4651 /label="pie1.3'" promoter complement(7826..7845) /label="T3_promoter" misc_feature complement(7862..7880) /label="M13r_primer" misc_feature complement(8011..8037) /label="lacI.C" rep_origin complement(8253..8872) /label="pBR322_origin" gene complement(9027..9887) /label="Ampicillin" /gene="Ampicillin" CDS complement(9027..9887) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(9929..9957) /label="Amp_promoter" rep_origin complement(10151..10457) /label="f1_origin" misc_feature 10616..10632 /label="M13f20_primer" misc_feature complement(10475..10635) /label="lacZ_a" promoter 10640..10658 /label="T7_promoter" ORIGIN 1 GAGCTCCACC GCGGTTTTGG CGCGGTGGCG GCCGCGCGGA AGATCTggaa gtaaaatatt 61 gtaatatttt ccctaaattt cacttaaaaa taaggatttt tgcaacgtaa aacctcaaaa 121 attcgctgaa attgacgatt tttgttcaaa attttgaaaa acagggttaa ttttagctga 181 aaatcttcaa ttttgccaga aattggcttt ttttccgtcg ccacacggag aacttgccaa 241 aaatgaagac ttttagccaa tttagccctg aaaattcgag ttttgggcga tttttggtct 301 gagaagccgt tttcgcagta tattatattt tctcgttaaa tttcatttta aaataaacat 361 tttgaacgta taaccccaaa aattcgctgc gcaatggatg atttttgtcc aaagttttga 421 taattttagc taaaaacctc gaattttggc aatttttcgt gccgcatcgg ccgaaagttg 481 actttttttg gaattatcat aatttaatgc acattttggt agtttatctc acataatctg 541 tctgcaggcg ggaaaaattt gagataaact actaaaagat gcaaaacaag gatgataatt 601 ccagaaaatc aacttcggac cgtctcgaca cggaaaattg agcgaaattg gagttttaaa 661 atgatttagc cctgaaaatt cgagaaattt aggcggtttt gggtctgaaa aggcgttttc 721 gcagtatttt ctcgctaaat ttcatttatt atcacataat atcagtactt aggcgaatat 781 cttgtagaaa tgttggtttt gttcgataat tctctacttt tagagtcgat tcacgagaaa 841 atgttgcacc atcttacttt tttcccttgt aaaacataga ttcttctgta caatgaaaga 901 gaacacaatt tcttcgtgaa gcaactcaaa aagtagagaa aattcgaaca aaacccaaca 961 tttctacaag atattcgtct aaatagtgat ataatgtgtt aataagcata ttttaaatga 1021 aatttagcga aaaaactgcg aaaatttgtc aaaaaagcga atttccatcc gatcagcAGA 1081 TCTCTAAAAG TTACATAAAA TTGAAAGTTT TGTGGGGAAA TTGTTTTAAA ACACGCCTAT 1141 GGTAATACGA GAACATAAAA TTCTGAGAAT GCGTATTACA CAACATATTC GACGCGCAAA 1201 ATATAACTAT ATCTCGTAGC GAAAACTACA GTAATCCTTA AATGACTACT GTAGCGATTG 1261 TGTCGATTTA CGGGCTCGAT TCTCGGAAAA AAAAATCATT TTTCAAATTT TGACAGCGAT 1321 ATTCAATTTT TCTTCGTTTT TTCGTATTTC TCAGTCATTT TTGTGCTTAT TTTTAATATT 1381 TTATTCATTA ATAAATTAAT TTAATTGAAA AACGTCCAGA CGTATTTTTA CATTTCCCGT 1441 CTATTTCCGT AATAGGCGGT TAATAACGTA GAAATACGTC TGTATGAGTT TACTCATCAA 1501 AATGACACGA ATATTGGACG TTTTTAAATC AAATTAATTT ATTAATAGGT AAAATATTAA 1561 AAAATAAACA CAAAAATGAC AAAGGAATAG GAAAACACGA AGAGAAATTG AGTATCGCTG 1621 TCAAAATTCG AAAAACGTGG ATTTTTCCAA GAATCGAGCC CGTAAATCGA CACATTCGCT 1681 ACAGTAGTCA TTTAAGGATT ATTGTAGTTT TCGCTACGAG ATACTTTGCG CGCCAAAtAt 1741 GTtGTGAAAT ACGCATTCTC AGAATTTTAT GTTCCCGTAA TACCACAATG ACCGAATATC 1801 ATAATAAAAA AATTCAAAAA CAATTTCTAG ATTTTATATG ATTTTtTGAA AATTGAAAAA 1861 ATCTCAGTTT TCACCTAATT ATATTTGAAt TACcGCCAAT TGAACTCGTT CGTTGGAGCG 1921 CGCTTGCATT ATTTTCATTA ATTAATTTTA TTAATttTCA TTATTTCACT GATTTTCTTC 1981 ATTTTTGAGG TTTTTTTTAT CGGGAAATGA AAGAAATATA CAAGAAAAAT GCAAAATGTT 2041 TATTAAAAAC TGAAATTAGT ATTTCTCGGA GTTTTAGGCA TTTTCAGTTA CTTTTTAATA 2101 AAGATTTTGC ATTTTTCTTG TATATTTCTT TCATTTTCCG ATTAAAAAAC CCCAAAAATG 2161 AAGAAAATCA GTGAAATAAT GAAAATTAAT TCAAATAACT AATGAAAATA ATGCAAGCGC 2221 GCTCCAACCA ACGAGTTCAA TTGGCGGTAA TTCAAATATA ATTAGGTGAA AACTGAGATT 2281 TTTTCAATTT TCAAAAAATC ATATAACATC TAGACAATTT TTTTGAATTT TTTTATCATG 2341 ATATTCGGTC ATTGTGTCCC CATAGGCATG TTTTAAAGCA ATTTCCACCT TTAAATAAAA 2401 TCGAGAAAAA ATGGCGTCAA AAGACATATG TAAAAGCTGT TTCTCTTTGT ATATTCACAC 2461 AGAATATAGC CCGATTTTGG AGGTGAAAAA CCGGTTTTTT TGTATTCTTT ACGCCACATA 2521 AAGTGATATG GAAGAGAAAA TCGACATATT TATGTACTTG GAGTACGAGT ACATTGGAGA 2581 AAAAGGGTGA ACTACTGGAA AATCTGGGAA ATTTCAGAAA AAAATTCCGA AAAATCTTTT 2641 TTGCTGAAAA AAAAATTTGT TCGAAATTTG GGAAATACTG GAAAATTTTC ATCAAAATTA 2701 AAGCGATTTT TAACGTTTTT GCTTGGGAGA TACTATTTGT AATTTTAATG TTTGGTGAGG 2761 ACAACAGAAA TGACCTTCAG CGTACGGAAA ACGATTAAAA GACACATTTG AATCGAAAAT 2821 AGCGTGAAAA ACATGAAAAT ATCGGAAAAA TAGCTTTAAA ATTGGATTTG AAAGCAAAAA 2881 ATGTATCAAA AATCGAGAAA AATGCTTGAA AAAGTTGAAA AACTCAAACA AAAAATCAAA 2941 AAAGGTTTTT TTAAAAATCT GTGAGTATCG CAACGAAAGC ACAACTTGAG GCATGCGCCT 3001 TTAAACCAAC CGTAACCAAA TTCGGAGGCA AAAAAAGATT TCTCGCTGTT TTTTCAGTTT 3061 TAAAATCACT TTTCACGTTT CTTTTCGCAG TAATTCGTTT GCAAAACATC TGAAAAAATT 3121 TTATTTTATA AGAAATTATA TAAAAATCGC TATAAATTGA GTTTTTGCCC CCAAATTCAG 3181 CACGGAGCAC TTCTCAATTA AATATAATTT TATTTTTTAA CATTGAAAGC TTTCATAAAA 3241 TTGAGCTTTG TTTGTAAATA ATCAGTGAAA AAACACAGAA ATCTTCAAAA AATAAAGAAA 3301 AAGTTTTTAA AAATCTGTGA GTCCCGCAAC GAAAATAAAA CTTTAGGCAT GCGCCTTTAA 3361 ACCAACCGTA ACCAAATTCG GCGGCAAAAA TGGATTTCTC GCCGTTTTTT CAGTTTTACA 3421 ATCACTTTTT AGGTTTCTTT TTGCAGTATT TCGTTTCCCA AACAATTAAA AATCAAATTT 3481 TCTTTTCCAG ATAGCTCAAA CAGGATCCTA TCGATTCGCG GCCGCTGTAC ACCCGGTAGA 3541 AAAAATGTCA AAAGGAGAGG AATTGTTCAC CGGCGTCGTT CCAATCCTCG TTGAACTCGA 3601 CGGCGACGTC AACGGGCACA AGTTTTCCGT CTCTGGAGAG GGAGAGGGAG ACGCAACCTA 3661 CGGCAAATTG ACCCTTAAAT TCATTTGCAC CACAGgtaag tttcgatttt tgctatcttt 3721 ttcacgattt ttactctctt tttctactaa ctaacctcct ttttgaacat tttttcagGA 3781 AAATTGCCAG TTCCATGGCC AACATTGGTC ACAACCTTCT GTTACGGCGT CCAATGCTTC 3841 TCGCGTTACC CCGATCACAT GAAAAGGCAC GACTTTTTTA AATCCGCAAT GCCAGAAGGA 3901 TACGTACAAG AACGCACAAT TTTCTTCAAA GATGACGGAA ACTACAAGAC AAGgtaagtt 3961 tttcccaact ttttcggact ttttgaccgc tttttatact aactaacgga ctttttcgtc 4021 ctttttcagG GCCGAAGTCA AGTTTGAAGG AGACACCCTC GTCAACCGCA TCGAACTTAA 4081 AGGAATTGAC TTCAAAGAAG ACGGAAACAT TCTCGGACAC AAACTCGAAT ACAACTACAA 4141 TTCTCACAAC GTTTACATAA TGGCCGACAA ACAAAAGAAC GGAATCAAGg taagtttcgc 4201 tttttgatga tttttggacc atttttagga atttttcgat actaactaac tatttttgcc 4261 tgctttttca gGTCAACTTC AAGATTCGTC ACAACATTGA GGACGGAAGC GTCCAACTTG 4321 CTGACCATTA CCAGCAAAAC ACCCCGATTG GCGACGGACC CGTCCTCCTT CCGGACAACC 4381 ACTACCTCTC AACACAGAGC GCCCTCTCGA AAGACCCGAA CGAGAAGAGg taagtttatc 4441 gtttttcgac gatttttacc catttttacg ccatttttga tactaactaa cgccttttta 4501 ccgatttttc agGGACCACA TGGTCCTTTT GGAATTTGTT ACGGCCGCAG GCATCACCCA 4561 CGGCATGGAC GAACTCTACA AATAGCATTC GTAGAATTCG CTAGCCGGCC ATACAAGTAA 4621 TCCGGATAAT TTTGCCGTAT TTTCCATATT Ttgttttgta tatttatcca ctcaccccct 4681 ctctttgtcc tgtgaatgaa cttgtgccaa aaaagccaaa aataatttac tttttttaaa 4741 gacatattaa aacttgaaaa cttggaacaa tacggtgcga aagcgaaaca gagacagaaa 4801 aatgaacaaa gagatccgtg aaaagtgaac gatgatttag gcttcttgtt gggcggcgtg 4861 gatgagagtg atgttgtctc ctttgagaag aattctgccg atcttgttgc gtcccttcgt 4921 cttcATGTTC ACCTCCTCGG CTTCGTCGAA CACGACATTC ATGAACTCGT CGAATCCGAT 4981 GATGTAGCCC TCGAGACGGT GGGTGACATC CTCGTACAAC CAGATTTGGA CCCGAGTGCG 5041 GTTTTGGAGA TATCTGAAAT TTGGGAAATT GTTTTCTACT GATTAGTGAA AAAATATTAC 5101 CTGAAGATGA GGTTCACAGG CTGAACCATC ACTTTGTTGA GCTTTCTCGT CGACATTTTT 5161 GCGGGATGAT CTGTAAAAAT CAATATATTT TTTGAAAGAC ACAAATTTTT AAGATAAATA 5221 TCAAGAAAAC TCGATTTTAG CAGCTATTTA AGCTCAAAAA CTACAATTTA TGGCTTTAAA 5281 ACATAATTTT TCACATTTTA CATAAATTTC AACGAAAAAA AATGCCAAAA AACTTCAAAT 5341 TTGAGCAGAA ATCGGCTATT TTCTCGACAA AAATAATTTC GAAAATTCGT TCAAAAAAGA 5401 TGAGTACGCA AGCACCGTGA CGCACAGTTG CGGACTTTGT AAACCGTACC CCTGCATAAA 5461 AAGGCGGAAT TTTGAATTTA ATAAAATGCA GTTTAGAACA AAGAAAAGCA CGGAGAGAAT 5521 TGTGATAACT AATTATTTGT ATTTAATTTT AAATACTACA TAAAACTGAA GATTGGAACG 5581 AGTTAAAGCT GGTATTTGCG GTTTATAGCC CCAAAAGTTA AGGCTTGTTA GGCAATTATC 5641 GATAAAAACA ACGAGAAATT CGTGAAATCG GCAAAAATTT ATAGAAAACG TTTGAATTAA 5701 ATTAAATTTA CGCTTTTGCC GCCAACACCT ATCGAGACCC TAGTTATAGG GGCGGAGCGT 5761 CATGGGAGCG CGCTTGCAAC TCGATTTCAA AAAGTTTCGC TGTTTGTACA ACATTCTCAG 5821 TTGTTCTTAA TCAGTTAAAT AAGTTTCAAA ATTTTCAAAA TCATAAAACA CAGTTAATGC 5881 TAAATTAAAG TCTAAAAAAT CAGGTGATAA GGGGTTTGGA CGACCAAAAA GCGACCTTCG 5941 CTCGAAAATT GATAAACTAA CTACACTTTT TCCGGAAAAA TGATGGAAAA ATCCTGATTT 6001 TCTTCGTTGA ATCAATTTTT GTTTCATTTT TAACACTTTC ACATGAAATA TTTCAGTTAA 6061 AATGCTCAAG CTAATTGGGT TTCTTATAAT TTTGGGTCTC GCAACGGCCG ACAAGTGCTC 6121 AGATTGTTTG GCTCAGGGGA AACAGTGAGT TGGAAGCTTA CAATTCTGAG AATTCGTATT 6181 GCGCAGCTTA TTTGACGCGC AAAATATGAA ATATCACGTA GCGAAAACTA CAGTAATTCT 6241 TTAAATAAGA CTACTGTAGT GCCTATGTCG ATTTACTGCC TCAACTTTCT TAATCAAGCT 6301 CGTAAATCGA CACCAGCTCT ACAGTAGTCA TAGTTTTCGC TACGAGATAT TTTGCGCGTC 6361 AAATATGGTG TGCAATACGC ATTTTCAGAA TTTTATGTTC TCGTAATATA CCTTTTTTCA 6421 GCTGGTGTGT TACTGTAAAT GCATGTGGAT TTACGCCATG TGTCACTACA ATTACAGTGA 6481 GTTTTTTTGA AGAAAAAAAA ATTACCCATT TGATCGCCAA TTCCATTTTT CAAAAATCAA 6541 TAAAAAAAAA AAATTTCGGC GATCAGAAAC GTTACAGAAA GCCCTAAACT GCCCGGAACT 6601 ACCAGATAAA GCCCACGCTT ACGATGATTC ATTTATGAGG AATAAGGTAT TGATCACACA 6661 TTCTGCTGCC TACAATATGG ACCCACAAAA GTGTTTTACG TAGGTTCACC TTCTAGATAA 6721 ACCTTAAAAA TCGATACTTT CAGTAGTAAA ATGCCTTCGA TGAAAGTTTC CAAAACAGTT 6781 AATGTCAATT GTGATAGATA TGGGCCAAGT GAGTTGTTTT ATTAGCCCAC GGATTTCTGG 6841 CTTCCCTCAT AAATTGAAAA GGAAGAGATT GCCGAACTAG ACCATTTTGG CTCGGCGCGT 6901 TGCGTGTCAC GTCGCGGCTC GATTGTAAAA CTAAATGTAT TGGTCCGTGT GGAGTACACG 6961 ACTTTCCCTT TCCGGCAGGC GATTGTCAAT GGAGCGCGAA AAATTCAATG AGGAAGGCCA 7021 GAACCCCGTG TCTGTAGTTT TCTCAAAAAG TGGCAAAAAC TCAATAATTG CCAATTTTTG 7081 ACTGCCAATA AAAATTTCAC TAAATTTATT AAATTTATTT TAAGTTTTAT TGAAATTTTT 7141 TTAAAATTTC CCCACTGGCG CTAAAAAATC CAATTTTCAG AAGCAATGTG CTTCAGCTTC 7201 ACGGCCATAG ATACTGTCCA AAAAGTGATT GCAATGACGT TTAGAGGGAC AGAAGGAGCA 7261 GCTCAATTGT CTGAAGAAAT TTTGGATTTC TTCCAGTAAG TTCGGGAAAT GGCAATTTAA 7321 AGTTTAATTG GATTTTTGTA TTTTTTCTTC TCACGACTAC AGTTTTGCAC ATTTTTATGT 7381 TATAAAAAAC CAAAAAAAAA TTTTTTTGCC TTTTTTCTCA GCTTACCACA AAACAATTTC 7441 GACCAAAAAT AAATTGAAAA AATTAAAAAA TTTTTTGCAC GGTTTTCGGA CTCAAAAAAA 7501 GCTGAATGTT CCTGGAACTA TTTGTAAAAT TCAAAATTCA AAATTTTCAC GAATTCTCTA 7561 AAAGGTTCCA GCAAATTCCA CAACTTTCTT GAATTTTTCA AAAAAGTTCT TGAGCATTCA 7621 ACAATTATTT TTGGAATTTT CCAAAGGTTA CAGGACGTTC CAATTTTTCA AAAAAATTTC 7681 TCGCAAAAAA TGTTTTCTCA GAAAATTTGA ATTTCCCTCC ACATAAAAGA TTGTTCTCAA 7741 AAAATTTGCA AACACACATT TTCAGCGGAA AAAAGGCGTT CTTCGACTCT GGAAGTGTAT 7801 TCGAATATGG TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTCCGAG CTTGGCGTAA 7861 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA 7921 GGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA 7981 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA 8041 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG 8101 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 8161 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 8221 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 8281 GGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 8341 GGACTATAAA GATACCAGGC GTTCCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 8401 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 8461 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 8521 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 8581 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 8641 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 8701 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 8761 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 8821 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 8881 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 8941 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 9001 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 9061 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTGC CCGTCGTGTA GATAACTACG 9121 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 9181 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 9241 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 9301 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 9361 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 9421 TGATCCCCCA TGTTGTGAAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 9481 AGTAAGTTGG CCGCAGTGTT ATCACTCATG CTTATGGCAG CACTGCATAA TTCTCTTACT 9541 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 9601 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 9661 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 9721 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 9781 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 9841 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 9901 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 9961 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAA 10021 ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT 10081 TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA 10141 GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC 10201 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA 10261 TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC 10321 CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG 10381 AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA 10441 CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCATTCAG GCTACGCAAC 10501 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAGGGGGGA 10561 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA 10621 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG AATTG //