LOCUS       Exported                2091 bp ds-DNA     circular SYN 20-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 20, 2026 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gap             25..44
                     /estimated_length=20
     promoter        80..184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             185..1045
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1216..1804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2012..2042
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2050..2066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggtacctaag tgtggctgcg gaacnnnnnn nnnnnnnnnn nnnngcacga cgtcaggtgg
       61 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1861 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1921 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1981 tctagaaaag cgggctttag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     2041 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctcatat g
//
