LOCUS 420lib_pETcon_SARS2_LP. 6861 bp ds-DNA circular 05-MAR-2026 DEFINITION synthetic circular DNA. ACCESSION urn.local...34muarj-4v SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT Imported using the Genbank importer. File name: 300lib_pETcon_SARS2_FLip.gb ApEinfo:methylated:1 FEATURES Location/Qualifiers source 1..2152 /label="source:synthetic DNA construct" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /note="/ApEinfo_fwdcolor=pink" /mol_type="other DNA" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=source:synthetic DNA construct" /note="/ApEinfo_revcolor=pink" /note="/locus_tag=source:synthetic DNA construct" /organism="synthetic DNA construct" /locus_tag="source:synthetic DNA construct" /label="source:synthetic DNA construct" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 64..80 /label="T3" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=T3" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="T3" /label="T3" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" 5'UTR complement(105..112) /label="Partial" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="/ApEinfo_fwdcolor=#ffd327" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Partial" /note="/ApEinfo_revcolor=#ffd327" /locus_tag="Partial" /label="Partial" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" promoter 105..707 /label="Bidirectional promoter" /ApEinfo_revcolor="#346ee0" /ApEinfo_fwdcolor="#346ee0" /note="/ApEinfo_fwdcolor=#346ee0" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Bidirectional promoter" /note="/ApEinfo_revcolor=#346ee0" /locus_tag="Bidirectional promoter" /label="Bidirectional promoter" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 112..112 /label="Geneious type: misc_marker" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Geneious type: misc_marker" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="Geneious type: misc_marker" /label="Geneious type: misc_marker" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" promoter complement(112..470) /label="promoter" /ApEinfo_revcolor="#346ee0" /ApEinfo_fwdcolor="#346ee0" /note="/ApEinfo_fwdcolor=#346ee0" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=promoter" /note="/ApEinfo_revcolor=#346ee0" /locus_tag="promoter" /label="promoter" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" promoter 496..707 /label="GAL1 minimal promoter" /ApEinfo_revcolor="#346ee0" /ApEinfo_fwdcolor="#346ee0" /note="/ApEinfo_fwdcolor=#346ee0" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=GAL1 minimal promoter" /note="/ApEinfo_revcolor=#346ee0" /locus_tag="GAL1 minimal promoter" /label="GAL1 minimal promoter" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 648..675 /label="TNS_o64" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=TNS_o64" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="TNS_o64" /label="TNS_o64" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 707..707 /label="Geneious type: misc_marker(1)" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Geneious type: misc_marker(1)" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="Geneious type: misc_marker(1)" /label="Geneious type: misc_marker(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" 5'UTR 707..763 /label="Partial (all but the last 4 bases)" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="/ApEinfo_fwdcolor=#ffd327" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Partial (all but the last 4 bases)" /note="/ApEinfo_revcolor=#ffd327" /locus_tag="Partial (all but the last 4 bases)" /label="Partial (all but the last 4 bases)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 727..750 /label="TNS_o29" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=TNS_o29" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="TNS_o29" /label="TNS_o29" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 727..750 /label="Gal1seq" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Gal1seq" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="Gal1seq" /label="Gal1seq" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 772..777 /label="EcoRI" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=EcoRI" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="EcoRI" /label="EcoRI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" 5'UTR 779..802 /label="5'UTR" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="/ApEinfo_fwdcolor=#ffd327" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=5'UTR" /note="/ApEinfo_revcolor=#ffd327" /locus_tag="5'UTR" /label="5'UTR" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS 803..1063 /label="CDS (Aga2p)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS (Aga2p)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS (Aga2p)" /label="CDS (Aga2p)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="MQLLRCFSIFSVIASVLAQELTTICEQIPSPTLESTPYSLSTTTILANGKAMQGVFEYYKSVTFVSNCGSHPSTTSKGSPINTQYVF" misc_feature 1065..1089 /label="primer: o4 Aga2p_fwd" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=primer: o4 Aga2p_fwd" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="primer: o4 Aga2p_fwd" /label="primer: o4 Aga2p_fwd" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS 1097..1132 /label="CDS (HA tag)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS (HA tag)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS (HA tag)" /label="CDS (HA tag)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="PYDVPDYALQAS" primer_bind 1098..1118 /label="oTNS_065" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=oTNS_065" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="oTNS_065" /label="oTNS_065" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 1120..1139 /label="o232" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=o232" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="o232" /label="o232" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 1120..1189 /label="Twist 5' overlap" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Twist 5' overlap" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="Twist 5' overlap" /label="Twist 5' overlap" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS 1133..1177 /label="CDS (GGS linker)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS (GGS linker)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS (GGS linker)" /label="CDS (GGS linker)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="GGGGSGGGGRGGGGS" misc_feature 1155..1162 /label="NotI" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=NotI" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="NotI" /label="NotI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 1169..1189 /label="oTNS_005" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=oTNS_005" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="oTNS_005" /label="oTNS_005" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_binding 1184..1189 /label="NdeI" /ApEinfo_revcolor="#ff4809" /ApEinfo_fwdcolor="#ff4809" /note="/ApEinfo_fwdcolor=#ff4809" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=NdeI" /note="/ApEinfo_revcolor=#ff4809" /locus_tag="NdeI" /label="NdeI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 1190..1789 /label="LP.8.1.1 RBD" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" misc_binding 1790..1795 /label="XhoI" /ApEinfo_revcolor="#ff4809" /ApEinfo_fwdcolor="#ff4809" /note="/ApEinfo_fwdcolor=#ff4809" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=XhoI" /note="/ApEinfo_revcolor=#ff4809" /locus_tag="XhoI" /label="XhoI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind complement(1790..1809) /label="TNS_o6" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=TNS_o6" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="TNS_o6" /label="TNS_o6" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS 1796..1807 /label="CDS" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS" /label="CDS" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="GGGS" CDS 1808..1837 /label="CDS (Myc tag)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS (Myc tag)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS (Myc tag)" /label="CDS (Myc tag)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="EQKLISEEDL" 3'UTR 1848..2152 /label="yeast mating factor alpha 3' UTR" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /note="/ApEinfo_fwdcolor=#f76ca1" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=yeast mating factor alpha 3' UTR" /note="/ApEinfo_revcolor=#008040" /locus_tag="yeast mating factor alpha 3' UTR" /label="yeast mating factor alpha 3' UTR" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind 1878..1907 /label="AG_211" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=AG_211" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="AG_211" /label="AG_211" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind complement(1878..1907) /label="AG_212" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=AG_212" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="AG_212" /label="AG_212" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 2153..2210 /label="IlluminaP5" /ApEinfo_revcolor="#00f900" /ApEinfo_fwdcolor="#00f900" /locus_tag="IlluminaP5" /label="IlluminaP5" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0" misc_feature 2211..2226 /label="N16" /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /locus_tag="N16" /label="N16" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0" misc_feature 2227..2234 /label="NotI" /ApEinfo_revcolor="#ff2600" /ApEinfo_fwdcolor="#ff2600" /locus_tag="NotI(1)" /label="NotI(1)" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0" source 2235..6861 /label="source:synthetic DNA construct" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /note="/ApEinfo_fwdcolor=pink" /mol_type="other DNA" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=source:synthetic DNA construct" /note="/ApEinfo_revcolor=pink" /note="/locus_tag=source:synthetic DNA construct" /organism="synthetic DNA construct" /locus_tag="source:synthetic DNA construct(1)" /label="source:synthetic DNA construct(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 2235..2240 /label="SacI" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=SacI" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="SacI" /label="SacI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" primer_bind complement(2248..2267) /label="T7" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="/ApEinfo_fwdcolor=#14c0bd" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=T7" /note="/ApEinfo_revcolor=#4ec02b" /locus_tag="T7" /label="T7" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 2287..2320 /label="Illumina P7 handle" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Illumina P7 handle" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="Illumina P7 handle" /label="Illumina P7 handle" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" rep_origin 2465..2920 /label="rep_origin" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /note="/ApEinfo_fwdcolor=#999999" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=rep_origin" /note="/ApEinfo_revcolor=#999999" /locus_tag="rep_origin" /label="rep_origin" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" misc_feature 2590..2595 /label="NaeI" /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /note="/ApEinfo_fwdcolor=#7eff74" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=NaeI" /note="/ApEinfo_revcolor=#7eff74" /locus_tag="NaeI" /label="NaeI" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" 3'UTR complement(2977..3022) /label="3'UTR" /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /note="/ApEinfo_fwdcolor=#f76ca1" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=3'UTR" /note="/ApEinfo_revcolor=#008040" /locus_tag="3'UTR" /label="3'UTR" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS complement(3023..3697) /label="Tyrosinase-related protein 1(YeastSelectable marker)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=Tyrosinase-related protein 1(YeastSelectable marker)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="Tyrosinase-related protein 1 (YeastSelectablemarker)" /label="Tyrosinase-related protein 1 (YeastSelectablemarker)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRKRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK*" 5'UTR complement(3698..3801) /label="5'UTR(1)" /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /note="/ApEinfo_fwdcolor=#ffd327" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=5'UTR(1)" /note="/ApEinfo_revcolor=#ffd327" /locus_tag="5'UTR(1)" /label="5'UTR(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" rep_origin complement(4230..4743) /label="rep_origin(1)" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /note="/ApEinfo_fwdcolor=#999999" /direction="LEFT" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=rep_origin(1)" /note="/ApEinfo_revcolor=#999999" /locus_tag="rep_origin(1)" /label="rep_origin(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" rep_origin complement(4234..4610) /label="rep_origin(2)" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /note="/ApEinfo_fwdcolor=#999999" /direction="LEFT" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=rep_origin(2)" /note="/ApEinfo_revcolor=#999999" /locus_tag="rep_origin(2)" /label="rep_origin(2)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" centromere 4623..4648 /label="CDEI fragment of chromosome VIcentromerefrom S.cerevisiae" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /note="/locus_tag=CDEI fragment of chromosome VIcentromerefrom S.cerevisiae" /note="/ApEinfo_fwdcolor=pink" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDEI fragment of chromosome VIcentromerefrom S.cerevisiae" /note="/ApEinfo_revcolor=pink" /locus_tag="CDEI fragment of chromosome VI centromerefromS.cerevisiae" /label="CDEI fragment of chromosome VI centromerefromS.cerevisiae" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" promoter 4812..4840 /label="promoter(1)" /ApEinfo_revcolor="#346ee0" /ApEinfo_fwdcolor="#346ee0" /note="/ApEinfo_fwdcolor=#346ee0" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=promoter(1)" /note="/ApEinfo_revcolor=#346ee0" /locus_tag="promoter(1)" /label="promoter(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" CDS 4882..5742 /label="CDS(1)" /ApEinfo_revcolor="#e9d024" /ApEinfo_fwdcolor="#e9d024" /note="/ApEinfo_fwdcolor=#e9d024" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=CDS(1)" /note="/ApEinfo_revcolor=#e9d024" /locus_tag="CDS(1)" /label="CDS(1)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" rep_origin 5890..6675 /label="rep_origin(3)" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /note="/ApEinfo_fwdcolor=#999999" /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0-1 0 -0.5} {} 0} width 5 offset 0" /note="/ApEinfo_label=rep_origin(3)" /note="/ApEinfo_revcolor=#999999" /locus_tag="rep_origin(3)" /label="rep_origin(3)" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0" ORIGIN 1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc 61 gaaattaacc ctcactaaag ggaacaaaag ctggtaccaa ttccttgaat tttcaaaaat 121 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc 181 accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat 241 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt 301 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact 361 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc 421 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa 481 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag 541 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg 601 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac 661 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa 721 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat cgaattccct 781 acttcataca ttttcaatta agatgcagtt acttcgctgt ttttcaatat tttctgttat 841 tgcttcagtt ttagcacagg aactgacaac tatatgcgag caaatcccct caccaacttt 901 agaatcgacg ccgtactctt tgtcaacgac tactattttg gccaacggga aggcaatgca 961 aggagttttt gaatattaca aatcagtaac gtttgtcagt aattgcggtt ctcacccctc 1021 aacaactagc aaaggcagcc ccataaacac acagtatgtt tttaaggaca atagctcgac 1081 gattgaaggt agatacccat acgacgttcc agactacgct ctgcaggcta gtggtggagg 1141 aggctctggt ggaggcggcc gcggaggcgg agggtcggct agccatatga acgttaccaa 1201 cttgtgtcca ttccatgaag ttttcaatgc tactactttc gcttctgttt acgcttggaa 1261 tagaactaga atctctaact gcgttgctga ctattctgtc ttgtacaatt ttgctccatt 1321 cttcgctttc aagtgctatg gtgtttctcc aactaagttg aacgatttgt gtttcaccaa 1381 cgtttacgcc gattcctttg ttattaaagg taacgaagtc tcccaaattg ctccaggtca 1441 aactggtaat attgccgatt acaattacaa gttgccagat gatttcaccg gttgtgttat 1501 tgcttggaac tctaacaagt tggattctaa gagatctggc aactacgatt actggtacag 1561 gtctttgcgt aagtccaaat tgaagccatt cgaaagagat atttccaccg aaatctatca 1621 agctggtaac aagccatgta aaggtaaagg tccaaactgt tacttcccat tggaatctta 1681 cggtttcaga ccaacttatg gtgttggtca tcaaccatac agagttgttg ttttgtcttt 1741 cgagttgttg catgctccag ctactgtttg tggtccaaag aaatctactc tcgagggggg 1801 cggttccgaa caaaagctta tttctgaaga ggacttgtaa tagagatctg ataacaacag 1861 tgtagatgta acaaaatcga ctttgttccc actgtacttt tagctcgtac aaaatacaat 1921 atacttttca tttctccgta aacaacatgt tttcccatgt aatatccttt tctatttttc 1981 gttccgttac caactttaca catactttat atagctattc acttctatac actaaaaaac 2041 taagacaatt ttaattttgc tgcctgccat atttcaattt gttataaatt cctataattt 2101 atcctattag tagctaaaaa aagatgaatg tgaatcgaat cctaagagaa ttAATGATAC 2161 GGCGACCACC GAGATCTACA CTCTTTCCCT ACACGACGCT CTTCCGATCT NNNNNNNNNN 2221 NNNNNNGCGG CCGCgagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc 2281 gtcgttagat cggaagagca cacgtctgaa ctccagtcac ttacaacgtc gtgactggga 2341 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 2401 taatagcgaa gaggcccgca ccgatcgcct ttcccaacag ttgcgcagcc tgaatggcga 2461 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 2641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 2701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 2761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 2821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 2881 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc 2941 ttacgcatct gtgcggtatt tcacaccgca tagatcggca agtgcacaaa caatacttaa 3001 ataaatacta ctcagtaata acctatttct tagcattttt gacgaaattt gctattttgt 3061 tagagtcttt tacaccattt gtctccacac ctccgcttac atcaacacca ataacgccat 3121 ttaatctaag cgcatcacca acattttctg gcgtcagtcc accagctaac ataaaatgta 3181 agctttcggg gctctcttgc cttccaaccc agtcagaaat cgagttccaa tccaaaagtt 3241 cacctgtccc acctgcttct gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag 3301 ctgcactgag tagtatgttg cagtcttttg gaaatacgag tcttttaata actggcaaac 3361 cgaggaactc ttggtattct tgccacgact catctccatg cagttggacg atatcaatgc 3421 cgtaatcatt gaccagagcc aaaacatcct ccttaggttg attacgaaac acgccaacca 3481 agtatttcgg agtgcctgaa ctatttttat atgcttttac aagacttgaa attttccttg 3541 caataaccgg gtcaattgtt ctctttctat tgggcacaca tataataccc agcaagtcag 3601 catcggaatc tagagcacat tctgcggcct ctgtgctctg caagccgcaa actttcacca 3661 atggaccaga actacctgtg aaattaataa cagacatact ccaagctgcc tttgtgtgct 3721 taatcacgta tactcacgtg ctcaatagtc accaatgccc tccctcttgg ccctctcctt 3781 ttcttttttc gaccgaatta attcttaatc ggcaaaaaaa gaaaagctcc ggatcaagat 3841 tgtacgtaag gtgacaagct atttttcaat aaagaatatc ttccactact gccatctggc 3901 gtcataactg caaagtacac atatattacg atgctgttct attaaatgct tcctatatta 3961 tatatatagt aatgtcgtga tctatggtgc actctcagta caatctgctc tgatgccgca 4021 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 4081 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 4141 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 4201 taggttaatg tcatgataat aatggtttct tagacggatc gcttgcctgt aacttacacg 4261 cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt 4321 atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag 4381 aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt 4441 tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta 4501 aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta 4561 atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt 4621 cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat 4681 ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat 4741 gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 4801 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 4861 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 4921 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 4981 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 5041 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 5101 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 5161 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 5221 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 5281 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 5341 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 5401 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 5461 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 5521 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 5581 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 5641 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 5701 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 5761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 5821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 5881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 5941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 6001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 6061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 6121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 6181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 6241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 6301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 6361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 6421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 6481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 6541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 6601 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 6661 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 6721 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 6781 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 6841 ccggctcgta tgttgtgtgg a //