LOCUS       Exported                9528 bp DNA     circular SYN 19-DEC-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             285..465
                     /label=LTR 5'
                     /note="color: #b4abac"
     LTR             1900..1914
                     /label=cPPT
                     /note="color: #ffef86"
     misc_feature    1945..1973
                     /label=BA168 - seq primer
                     /note="color: #85dae9"
     primer_bind     1945..1972
                     /label=117-watermelon_lib_dialout_F
                     /note="color: black; sequence: 
                     AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTg
                     tagacataatagcaacagacatacaaa"
     misc_feature    2032..2089
                     /label=ANTIPARALLEL - BA167 - Gibson primer
                     /note="color: #f8d3a9"
     misc_feature    2066..2071
                     /label=XbaI
                     /note="color: #faac61"
     polyA_signal    complement(2072..2299)
                     /label=ANTIPARALLEL - BGH pA
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    2182..2187
                     /label=5' (RNA) is wt cleavage site and 3' is variantc
                     /label=5' (RNA) is wt cleavage site and 3' is 
                     variantcleavage site
                     /note="color: #f58a5e"
     misc_feature    2201..2206
                     /label=""
                     /note="color: #c6c9d1"
     primer_bind     2208..2227
                     /label=5-flow_cell_adaptor_F
                     /note="color: black; sequence: 
                     AATGATACGGCGACCACCGAGATCTACACTCTCGGAAAGGACAGTGGGAGTGG"
     primer_bind     2235..2252
                     /label=31-P004_insertPCR_verifier_F
                     /note="color: black; sequence: CAGGGTCAAGGAAGGCAC"
     primer_bind     2272..2290
                     /label=33-P004/P005_insertPCR_verifier_F
                     /note="color: black; sequence: GATGGCTGGCAACTAGAAG"
     primer_bind     2275..2332
                     /label=26-barcode amplifier -F
                     /note="color: black; sequence: 
                     GGCTGGCAACTAGAAGGCACAGTCGAGGCTGATCAGCGGGTTTCCTGCAGGGTTTGTC"
     primer_bind     2295..2312
                     /label=35-amplify full insert-F
                     /note="color: black; sequence: AGTCGAGGCTGATCAGCG"
     primer_bind     2306..2335
                     /label=64-Sequencing_Primer_1
                     /note="color: black; sequence: 
                     ATCAGCGGGTTTCCTGCAGGGTTTGTCTTC"
     primer_bind     complement(2313..2402)
                     /label=63-barcode primer mNeon-R
                     /note="color: black; sequence: 
                     CTGTACAAGTAAAttataaCCTGCAGGATGCGTCTTCSWSWSWSWSWSWSWSWSWSWSW
                     SWSWSWSWGAAGACAAACCCTGCAGGAAACC"
     misc_feature    2318..2325
                     /label=Sbf1 site
                     /note="color: #0000ff"
     misc_feature    2330..2335
                     /label=Bbs1 site
                     /note="color: #800080"
     misc_feature    2336..2365
                     /label=expressed semi-random barcode
                     /note="color: #ff00ff"
     primer_bind     complement(2366..2390)
                     /label=95-Sequencing_Primer_1_R_long
                     /note="color: black; sequence: AttataaCCTGCAGGATGCGTCTTC"
     misc_feature    2366..2371
                     /label=Bbs1 site
                     /note="color: #800080"
     primer_bind     complement(2371..2390)
                     /label=118-watermelon_lib_dialout_R
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGA
                     ttataaCCTGCAGGATGCG"
     misc_feature    2376..2383
                     /label=Sbf1 site
                     /note="color: #0000ff"
     misc_feature    2387..2389
                     /label=RS-1(Psi1)
                     /note="color: #ffcc99"
     CDS             complement(2391..3101)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /note="color: #05fd14"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     primer_bind     complement(2408..2430)
                     /label=100-Primer_2_10X_WTA__closer_R
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATATCACGGTGACTGGAGTTCAGACGTGTGCTCTTCC
                     GATCTTTACCGATGTGATGGGCATGG"
     primer_bind     complement(2439..2462)
                     /label=64-flow_cell_adaptor_R_v1
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATAAGCAACTGAGCTCAACTTCAAGGAGTGG"
     primer_bind     complement(2460..2480)
                     /label=94-Primer_2_10X_WTA_R
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATATCACGGTGACTGGAGTTCAGACGTGTGCTCTTCC
                     GATCTGAGCTCAAGCACTCCAAGACC"
     primer_bind     complement(2538..2557)
                     /label=113-Increase_barcodeRecovery_for10X
                     /note="color: black; sequence: GGACCACCTACACCTTTGCC"
     CDS             complement(3102..3128)
                     /codon_start=1
                     /label=NLS
                     /note="color: #800000"
                     /translation="MAPKKKRKV"
     misc_feature    3131..3138
                     /label=RS-2(Not1)
                     /note="color: #ffcc99"
     misc_feature    3141..3203
                     /label=ANTIPARALLEL - t2a
                     /note="color: #faac61"
     primer_bind     3162..3181
                     /label=54-T2A_F
                     /note="color: black; sequence: GTCACCGCATGTTAGCAGAC"
     CDS             complement(3204..3947)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3204..3210
                     /label=RS-3(Sma1)
                     /note="color: #ffcc99"
     misc_feature    3966..3971
                     /label=RS-4(NhE1)
                     /note="color: #ffcc99"
     promoter        complement(3972..4482)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ccffff; direction: LEFT"
     primer_bind     4099..4116
                     /label=55-hPGK_promoter-F
                     /note="color: black; sequence: TCCCGCACCGCCCCTTCC"
     primer_bind     4475..4496
                     /label=62-verify_TRE_promotor-F
                     /note="color: black; sequence: CCAACCCCGTGGAATTATCACC"
     promoter        4499..4866
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    4507..4525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4543..4561
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4579..4597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4615..4633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4651..4669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4687..4705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4723..4741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    4868..4872
                     /label=RS-5(EcoR1)
                     /note="color: #ffcc99"
     primer_bind     complement(4872..4889)
                     /label=57-amplify_gBlockA-R
                     /note="color: black; sequence: GTTCAGGCATGGTGGTGG"
     misc_feature    4874..4879
                     /label=Kozak
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4880..5986
                     /codon_start=1
                     /label=H2BR1mut-mCherry CDS
                     /note="H2BR1mut-mCherry CDS"
                     /note="color: #ff0000"
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSKDPPVATMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHE
                     FEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKL
                     SFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWE
                     ASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITS
                     HNEDYTIVEQYERAEGRHSTGGMDELYK"
     misc_feature    5069..5071
                     /label=mutated from AAT
                     /note="mutated from AAT"
                     /note="color: #a6acb3"
     misc_feature    5632
                     /label=changed from g
                     /note="color: #a6acb3"
     primer_bind     5781..5799
                     /label=58-h2B-mCherry-F
                     /note="color: black; sequence: ggctgaagctgaaggacgg"
     misc_feature    5987..5994
                     /label=RS-6(PME1)
                     /note="color: #ffcc99"
     misc_feature    5995..6583
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    6584..6588
                     /label=RS-7(Kpn1)
                     /note="color: #ffcc99"
     primer_bind     complement(6602..6621)
                     /label=59-amplify_bBlockB-R
                     /note="color: black; sequence: ctacagctgccttgtaagtc"
     LTR             6638..6652
                     /label=cPPT
                     /note="color: #d59687"
     LTR             6975..7155
                     /label=3' LTR
                     /note="color: #85dae9"
     rep_origin      7199..7881
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     gene            complement(7979..8638)
                     /label=AmpR
                     /note="color: #f58a5e; direction: LEFT"
     promoter        8878..8906
                     /label=AmpR promoter
                     /note="color: #d6b295"
     promoter        join(9232..9528,1..267)
                     /label=pU6 5'CMV promoter
                     /note="color: #d59687"
ORIGIN
        1 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
       61 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      121 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      181 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      241 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      301 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      361 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      421 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      481 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      541 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      601 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      661 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      721 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      781 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      841 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      901 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      961 caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga gatatgaggg
     1021 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1081 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1141 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1201 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1261 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1321 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1381 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1441 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1501 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1561 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1621 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1681 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1741 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1801 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     1861 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     1921 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     1981 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2041 ccagtttggt tagtaccggg cccgctctag accatagagc ccaccgcatc cccagcatgc
     2101 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     2161 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     2221 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     2281 caactagaag gcacagtcga ggctgatcag cgggtttcct gcagggtttg tcttcwswsw
     2341 swswswswsw swswswswsw swswsgaaga cgcatcctgc aggttataat ttacttgtac
     2401 agctcgtcca tgcccatcac atcggtaaag gccttttgcc actccttgaa gttgagctcg
     2461 gtcttggagt gcttgagctc cgtcttacgg aacacgtaca tcggctggtt cttcagatag
     2521 ttagccgcca ttggcttggc aaaggtgtag gtggtccgcg cagtgctccg gtagcgcttg
     2581 ccatttccag tggtgtaact ccacttaaag gtactgatga tggttttgtc gttggggtaa
     2641 gtcttcttcg acctgcacca gtccgcagcg gtcagcgagt tggtcatcac aggaccgtca
     2701 gcagggaaac cagtcccctt cacctgggcc tctcctttga tgtggcttcc ctcgtaggtg
     2761 tagcggtagt taacagtaag ggaggcacca tcttcaaact gcattgtgcg atggacttgg
     2821 tatccggagc catctaccat ggcggcctgg aaaggcgaca tcccgtcagg gtagggcagg
     2881 tactgatgga agccataccc gatatgaggg accagaatcc agggggagaa ctggaggtca
     2941 cccttggtgg acttcaggtt taactcctca taaccatcat ttggattgcc ggtgccctga
     3001 cccaccatgt caaagtccac accgttgatg gagccaaaga tgtgtaactc atgtgtcgct
     3061 gggagagagg ccatgttatc ctcctcgccc ttgctcacca ttaccttacg cttcttcttt
     3121 ggagccatgt gcggccgcgt tgggccagga ttctcctcga cgtcaccgca tgttagcaga
     3181 cttcctctgc cctctcccga tcccccgggg agcatgtcaa ggtcaaaatc gtcaagagcg
     3241 tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag
     3301 tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg
     3361 ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac
     3421 agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc
     3481 tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg
     3541 gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc
     3601 tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc
     3661 ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct
     3721 tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt
     3781 ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca
     3841 actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt
     3901 aattccagag cagagtttat gactttgctc ttgtccagtc tagacatggt aattcgatga
     3961 tccccgctag cctggggaga gaggtcggtg attcggtcaa cgagggagcc gactgccgac
     4021 gtgcgctccg gaggcttgca gaatgcggaa caccgcgcgg gcaggaacag ggcccacact
     4081 accgccccac accccgcctc ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct
     4141 gctgagcagc cgctattggc cacagcccat cgcggtcggc gcgctgccat tgctccctgg
     4201 cgctgtccgt ctgcgagggt actagtgaga cgtgcggctt ccgtttgtca cgtccggcac
     4261 gccgcgaacc gcaaggaacc ttcccgactt aggggcggag caggaagcgt cgccgggggg
     4321 cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga
     4381 gacccagggt cggcgccgct gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc
     4441 gcaaacccag ggctgccttg gaaaaggcgc aaccccaacc ccgtggaatt atcacctcga
     4501 gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     4561 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     4621 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     4681 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     4741 aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg attttttgag
     4801 taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac ttcctaccct
     4861 cgtaaagaat tccaccacca tgcctgaacc ctctaagtct gctccagccc ctaaaaaggg
     4921 ttctaagaag gctatcacta aggcgcagaa gaaggatggt aagaagcgta agcgcagccg
     4981 caaggagagc tattctatct atgtgtacaa ggttctgaag caggtccacc ccgacaccgg
     5041 catctcatcc aaggccatgg ggatcatgaa ctccttcgtc aacgacatct tcgagcgcat
     5101 cgcgggcgag gcttctcgcc tggctcacta caataagcgc tcgaccatca cctccaggga
     5161 gattcagacg gctgtgcgcc tgctgctgcc tggggagctg gctaagcatg ctgtgtccga
     5221 gggcactaag gcagttacca agtacactag ctctaaggat ccaccggtcg ccaccatggt
     5281 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     5341 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5401 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     5461 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     5521 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5581 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aagacggcga
     5641 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     5701 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     5761 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     5821 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     5881 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     5941 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagttt aaacaatcaa
     6001 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6061 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6121 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6181 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6241 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6301 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6361 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6421 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6481 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6541 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcggtacct ttaagaccaa
     6601 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6661 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6721 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     6781 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     6841 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6901 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     6961 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7141 ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8941 gcacatttcc ccgaaaagtg ccacctgacg cttaagcggt cgacggatcg ggagatctcc
     9001 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9061 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     9121 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     9181 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     9241 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     9301 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9361 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9421 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     9481 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgccca
//
