LOCUS Exported 9168 bp DNA circular SYN 26-JUL-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9168) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 26, 2021 from SnapGene 5.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9168 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 2962..3227 /label=hU6 promoter /note="hU6 promoter" misc_feature 3582..3887 /label=ccdB /note="ccdB" misc_feature 3940..4032 /label=iCRISPR scaffold /note="iCRISPR scaffold" misc_feature 4039..4586 /label=mPGK promoter /note="mPGK promoter" misc_feature 4587..5255 /label=Puro-2A-BFP /note="Puro-2A-BFP" misc_feature 5256..5783 /label=Thy1.1 /note="Thy1.1" ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac 661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc 1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg 1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct 1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa 1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac 1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta 1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg 1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg 1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg 1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag 1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga 1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca 1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga 1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac 2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa 2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat 2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg 2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc 2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga 2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc 2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt 2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa 2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg 2941 ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc 3001 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg 3061 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 3121 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt 3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttccgtaa 3241 tctagaggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 3301 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 3361 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 3421 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 3481 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 3541 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 3601 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 3661 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtcc 3721 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 3781 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 3841 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 3901 ttatacacag ccagtctgca ggtcgaccat agaagacacg tttaagagct atgctggaaa 3961 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc 4021 ggtgcttttt ttggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg 4081 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc 4141 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg 4201 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt 4261 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg 4321 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg 4381 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca 4441 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc 4501 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc 4561 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 4621 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 4681 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 4741 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 4801 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 4861 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 4921 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 4981 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 5041 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 5101 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 5161 gcatgacccg caagcccggt gccggcggcg ggtccggagg agagggcaga ggaagtctcc 5221 taacatgcgg tgacgtggag gagaatcctg gcccaaccat gggtcttttc tgcagtcacc 5281 gtcctcgagg caccatgaac ccagccatca gcgtcgctct cctgctctca gtcttgcagg 5341 tgtcccgagg gcagaaggtg accagcctga cagcctgcct ggtgaaccaa aaccttcgcc 5401 tggactgccg ccatgagaat aacaccaagg ataactccat ccagcatgag ttcagcctga 5461 cccgagagaa gaggaagcac gtgctctcag gcacccttgg gatacccgag cacacgtacc 5521 gctcccgcgt caccctctcc aaccagccct atatcaaggt ccttacccta gccaacttca 5581 ccaccaagga tgagggcgac tacttttgtg agcttcgcgt ctcgggcgcg aatcccatga 5641 gctccaataa aagtatcagt gtgtatagag acaagctggt caagtgtggc ggcataagcc 5701 tgctggttca gaacacatcc tggatgctgc tgctgctgct ttccctctcc ctcctccaag 5761 ccctggactt catttctctg tgagcggccg ctcaacctct ggattacaaa atttgtgaaa 5821 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5881 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5941 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 6001 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 6061 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 6121 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 6181 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 6241 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 6301 tgccggctct gcggcctctt ccgcgtctac gccttcgccc tcagacgagt cggatctccc 6361 tttgggccgc ctccccgcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6421 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagtc 6481 aagatctgct ttttgcctgt actgggtctc tctggttaga ccagagtctc tctggttaga 6541 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6601 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6661 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtttaa acccgctgat 6721 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 6781 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 6841 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 6901 gggaggattg ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atgggcggcc 6961 gttaattaag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt 7021 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7081 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7141 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7201 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7321 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7861 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //