LOCUS Peft_3_Cas9_tbb_ 7799 bp ds-DNA circular 21-JUN-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 1037..5227 /label=Cas9 open reading frame with intervening intron and /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 3113..3163 /label=intron sequence /ApEinfo_fwdcolor=#ff121b /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 402..1010 /label=eft-3 promoter /ApEinfo_fwdcolor=#15ff49 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 5228..5559 /label=tbb-2 3'UTR /ApEinfo_fwdcolor=#6501ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 5965..6579 /label=pMB1 Replication origin /ApEinfo_fwdcolor=#ff951c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6739..7599 /label=AmpR /ApEinfo_fwdcolor=#ff14e7 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_signal 5192..5227 /label=SV40 NLS /ApEinfo_fwdcolor=#a8a8ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 1018..1031 /label=attB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGCACCTTTG GTCTTTTATT 421 GTCAACTTCC ATTGGTTCTT CCATTGTTTC TGTTAAATTA ATGAATTTTT CATAAAATAA 481 AGACATTATA CAATATAAAA ATGAAGAATT TATTGAAAAT AAACTGCCAG AGAGAAAAAG 541 TATGCAACAC TCCCGCCGAG AGTGTTTGAA ATGGTGTACG GTACATTTTC GTGCTAGGAG 601 TTAGATGTGC AGGCAGCAAC GAGAGGGGGA GAGATTTTTT TGGGCCTTGT GAAATTAACG 661 TGAGTTTTCT GGTCATCTGA CTAATCATGT TGGTTTTTTG TTGGTTTATT TTGTTTTTAT 721 CTTTGTTTTT ATCCAGATTA GGAAATTTAA ATTTTATGAA TTTATAATGA GGTCAAACAT 781 TCAGTCCCAG CGTTTTTCCT GTTCTCACTG TTTAGTCGAA TTTTTATTTT AGGCTTTCAA 841 CAAATGTTCT AACTGTCTTA TTTGTGACCT CACTTTTTAT ATTTTTTTAA TTTTTAAAAA 901 TATTAGAAGT TTCTAGGATA ATTTTTTCGA CTTTTATTCT CTCTACCGTC CGCACTCTTC 961 TTACTTTTAA ATTAAATTGT TTTTTTTTCA GTTGGGAAAC ACTTTGCTCA caagtttgta 1021 caaaaaagca ggcttaATGG ACAAGAAGTA CTCCATCGGA CTCGACATCG GAACCAACTC 1081 CGTCGGATGG GCCGTCATCA CCGACGAGTA CAAGGTCCCA TCCAAGAAGT TCAAGGTCCT 1141 CGGAAACACC GACCGTCACT CCATCAAGAA GAACCTCATC GGAGCCCTCC TCTTCGACTC 1201 CGGAGAGACC GCCGAGGCCA CCCGTCTCAA GCGTACCGCC CGTCGTCGTT ACACCCGTCG 1261 TAAGAACCGT ATCTGCTACC TCCAGGAGAT CTTCTCCAAC GAGATGGCCA AGGTCGACGA 1321 CTCCTTCTTC CACCGTCTCG AGGAGTCCTT CCTCGTCGAG GAGGACAAGA AGCACGAGCG 1381 TCACCCAATC TTCGGAAACA TCGTCGACGA GGTCGCCTAC CACGAGAAGT ACCCAACCAT 1441 CTACCACCTC CGTAAGAAGC TCGTCGACTC CACCGACAAG GCCGACCTCC GTCTCATCTA 1501 CCTCGCCCTC GCCCACATGA TCAAGTTCCG TGGACACTTC CTCATCGAGG GAGACCTCAA 1561 CCCAGACAAC TCCGACGTCG ACAAGCTCTT CATCCAGCTC GTCCAGACCT ACAACCAGCT 1621 CTTCGAGGAG AACCCAATCA ACGCCTCCGG AGTCGACGCC AAGGCCATCC TCTCCGCCCG 1681 TCTCTCCAAG TCCCGTCGTC TCGAGAACCT CATCGCCCAG CTCCCAGGAG AGAAGAAGAA 1741 CGGACTCTTC GGAAACCTCA TCGCCCTCTC CCTCGGACTC ACCCCAAACT TCAAGTCCAA 1801 CTTCGACCTC GCCGAGGACG CCAAGCTCCA GCTCTCCAAG GACACCTACG ACGACGACCT 1861 CGACAACCTC CTCGCCCAGA TCGGAGACCA GTACGCCGAC CTCTTCCTCG CCGCCAAGAA 1921 CCTCTCCGAC GCCATCCTCC TCTCCGACAT CCTCCGTGTC AACACCGAGA TCACCAAGGC 1981 CCCACTCTCC GCCTCCATGA TCAAGCGTTA CGACGAGCAC CACCAGGACC TCACCCTCCT 2041 CAAGGCCCTC GTCCGTCAGC AGCTCCCAGA GAAGTACAAG GAGATCTTCT TCGACCAGTC 2101 CAAGAACGGA TACGCCGGAT ACATCGACGG AGGAGCCTCC CAGGAGGAGT TCTACAAGTT 2161 CATCAAGCCA ATCCTCGAGA AGATGGACGG AACCGAGGAG CTCCTCGTCA AGCTCAACCG 2221 TGAGGACCTC CTCCGTAAGC AGCGTACCTT CGACAACGGA TCCATCCCAC ACCAGATCCA 2281 CCTCGGAGAG CTCCACGCCA TCCTCCGTCG TCAGGAGGAC TTCTACCCAT TCCTCAAGGA 2341 CAACCGTGAG AAGATCGAGA AGATCCTCAC CTTCCGTATC CCATACTACG TCGGACCACT 2401 CGCCCGTGGA AACTCCCGTT TCGCCTGGAT GACCCGTAAG TCCGAGGAGA CCATCACCCC 2461 ATGGAACTTC GAGGAGGTCG TCGACAAGGG AGCCTCCGCC CAGTCCTTCA TCGAGCGTAT 2521 GACCAACTTC GACAAGAACC TCCCAAACGA GAAGGTCCTC CCAAAGCACT CCCTCCTCTA 2581 CGAGTACTTC ACCGTCTACA ACGAGCTCAC CAAGGTCAAG TACGTCACCG AGGGAATGCG 2641 TAAGCCAGCC TTCCTCTCCG GAGAGCAGAA GAAGGCCATC GTCGACCTCC TCTTCAAGAC 2701 CAACCGTAAG GTCACCGTCA AGCAGCTCAA GGAGGACTAC TTCAAGAAGA TCGAGTGCTT 2761 CGACTCCGTC GAGATCTCCG GAGTCGAGGA CCGTTTCAAC GCCTCCCTCG GAACCTACCA 2821 CGACCTCCTC AAGATCATCA AGGACAAGGA CTTCCTCGAC AACGAGGAGA ACGAGGACAT 2881 CCTCGAGGAC ATCGTCCTCA CCCTCACCCT CTTCGAGGAC CGTGAGATGA TCGAGGAGCG 2941 TCTCAAGACC TACGCCCACC TCTTCGACGA CAAGGTCATG AAGCAGCTCA AGCGTCGTCG 3001 TTACACCGGA TGGGGACGTC TCTCCCGTAA GCTCATCAAC GGAATCCGTG ACAAGCAGTC 3061 CGGAAAGACC ATCCTCGACT TCCTCAAGTC CGACGGATTC GCCAACCGTA ACGTAAGTTT 3121 AAACATGATT TTACTAACTA ACTAATCTGA TTTAAATTTT CAGTTCATGC AGCTCATCCA 3181 CGACGACTCC CTCACCTTCA AGGAGGACAT CCAGAAGGCC CAGGTCTCCG GACAGGGAGA 3241 CTCCCTCCAC GAGCACATCG CCAACCTCGC CGGATCCCCA GCCATCAAGA AGGGAATCCT 3301 CCAGACCGTC AAGGTCGTCG ACGAGCTCGT CAAGGTCATG GGACGTCACA AGCCAGAGAA 3361 CATCGTCATC GAGATGGCCC GTGAGAACCA GACCACCCAG AAGGGACAGA AGAACTCCCG 3421 TGAGCGTATG AAGCGTATCG AGGAGGGAAT CAAGGAGCTC GGATCCCAGA TCCTCAAGGA 3481 GCACCCAGTC GAGAACACCC AGCTCCAGAA CGAGAAGCTC TACCTCTACT ACCTCCAGAA 3541 CGGACGTGAC ATGTACGTCG ACCAGGAGCT CGACATCAAC CGTCTCTCCG ACTACGACGT 3601 CGACCACATC GTCCCACAGT CCTTCCTCAA GGACGACTCC ATCGACAACA AGGTCCTCAC 3661 CCGTTCCGAC AAGAACCGTG GAAAGTCCGA CAACGTCCCA TCCGAGGAGG TCGTCAAGAA 3721 GATGAAGAAC TACTGGCGTC AGCTCCTCAA CGCCAAGCTC ATCACCCAGC GTAAGTTCGA 3781 CAACCTCACC AAGGCCGAGC GTGGAGGACT CTCCGAGCTC GACAAGGCCG GATTCATCAA 3841 GCGTCAGCTC GTCGAGACCC GTCAGATCAC CAAGCACGTC GCCCAGATCC TCGACTCCCG 3901 TATGAACACC AAGTACGACG AGAACGACAA GCTCATCCGT GAGGTCAAGG TCATCACCCT 3961 CAAGTCCAAG CTCGTCTCCG ACTTCCGTAA GGACTTCCAG TTCTACAAGG TCCGTGAGAT 4021 CAACAACTAC CACCACGCCC ACGACGCCTA CCTCAACGCC GTCGTCGGAA CCGCCCTCAT 4081 CAAGAAGTAC CCAAAGCTCG AGTCCGAGTT CGTCTACGGA GACTACAAGG TCTACGACGT 4141 CCGTAAGATG ATCGCCAAGT CCGAGCAGGA GATCGGAAAG GCCACCGCCA AGTACTTCTT 4201 CTACTCCAAC ATCATGAACT TCTTCAAGAC CGAGATCACC CTCGCCAACG GAGAGATCCG 4261 TAAGCGTCCA CTCATCGAGA CCAACGGAGA GACCGGAGAG ATCGTCTGGG ACAAGGGACG 4321 TGACTTCGCC ACCGTCCGTA AGGTCCTCTC CATGCCACAG GTCAACATCG TCAAGAAGAC 4381 CGAGGTCCAG ACCGGAGGAT TCTCCAAGGA GTCCATCCTC CCAAAGCGTA ACTCCGACAA 4441 GCTCATCGCC CGTAAGAAGG ACTGGGACCC AAAGAAGTAC GGAGGATTCG ACTCCCCAAC 4501 CGTCGCCTAC TCCGTCCTCG TCGTCGCCAA GGTCGAGAAG GGAAAGTCCA AGAAGCTCAA 4561 GTCCGTCAAG GAGCTCCTCG GAATCACCAT CATGGAGCGT TCCTCCTTCG AGAAGAACCC 4621 AATCGACTTC CTCGAGGCCA AGGGATACAA GGAGGTCAAG AAGGACCTCA TCATCAAGCT 4681 CCCAAAGTAC TCCCTCTTCG AGCTCGAGAA CGGACGTAAG CGTATGCTCG CCTCCGCCGG 4741 AGAGCTCCAG AAGGGAAACG AGCTCGCCCT CCCATCCAAG TACGTCAACT TCCTCTACCT 4801 CGCCTCCCAC TACGAGAAGC TCAAGGGATC CCCAGAGGAC AACGAGCAGA AGCAGCTCTT 4861 CGTCGAGCAG CACAAGCACT ACCTCGACGA GATCATCGAG CAGATCTCCG AGTTCTCCAA 4921 GCGTGTCATC CTCGCCGACG CCAACCTCGA CAAGGTCCTC TCCGCCTACA ACAAGCACCG 4981 TGACAAGCCA ATCCGTGAGC AGGCCGAGAA CATCATCCAC CTCTTCACCC TCACCAACCT 5041 CGGAGCCCCA GCCGCCTTCA AGTACTTCGA CACCACCATC GACCGTAAGC GTTACACCTC 5101 CACCAAGGAG GTCCTCGACG CCACCCTCAT CCACCAGTCC ATCACCGGAC TCTACGAGAC 5161 CCGTATCGAC CTCTCCCAGC TCGGAGGAGA CTCCCGTGCC GACCCAAAGA AGAAGCGTAA 5221 GGTCTGAGAT AAATGCAAAA TCCTTTCAAG CATTCCCTTC TTCTCTATCA CTCTTCTTTC 5281 TTTTTGTCAA AAAATTCTCT CGCTAATTTA TTTGCTTTTT TAATGTTATT ATTTTATGAC 5341 TTTTTATAGT CACTGAAAAG TTTGCATCTG AGTGAAGTGA ATGCTATCAA AATGTGATTC 5401 TGTCTGATGT ACTTTCACAA TCTCTCTTCA ATTCCATTTT GAAGTGCTTT AAACCCGAAA 5461 GGTTGAGAAA AATGCGAGCG CTCAAATATT TGTATTGTGT TCGTTGAGTG ACCCAACAAA 5521 AAGAGGAAAC TTTATTGTGC CGCCAAGAAA AAAGTCTCAA AGCTTGGCGT AATCATGGTC 5581 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 5641 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 5701 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 5761 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 5821 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 5881 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 5941 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 6001 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 6061 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 6121 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 6181 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 6241 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 6301 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 6361 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 6421 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 6481 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 6541 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 6601 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 6661 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 6721 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 6781 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 6841 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 6901 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 6961 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 7021 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 7081 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 7141 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 7201 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 7261 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 7321 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 7381 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 7441 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 7501 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 7561 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 7621 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 7681 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA 7741 AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC //