LOCUS       Ple47\(pEMS1152)       20148 bp    DNA     circular      3-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS3045 - 5'  TCACAGTTTGGGCACTGCAGA - 3' Tm=56.5 Deg C
            Antisense primer: oEMS3037 - 5'  TCAAAGTGCAGTCCCAGGCA - 3 Tm=56.4 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            459 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543241538|
COMMENT     VNTDBDATE|544966149|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple47 (pEMS1152)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT          #138=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #139=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT                1 3 "@T @N " 0) 
COMMENT          #140=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
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COMMENT                            1.74233 0.833333 0 "Ple47 (pEMS1152)" "@N" 1 0 
COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
COMMENT                      #156=(CLabel 
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COMMENT                            (LOGPEN 0 0 0) 1 
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COMMENT               #157=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1092 100) 
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COMMENT                                   7.99245 1.17389 #159#) 
COMMENT                             #161=(CWideArrow 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   13.0756 -1.74233 0 -10 2.67247 1.17389 #161#) 
COMMENT                             #163=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 16.3877 
COMMENT                                   -3.48466 0 -10 10.0405 1.17389 #163#) 
COMMENT                             #165=(CScratch 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 17.3148 
COMMENT                                   -8.71165 0 -10 11.0645 1.17389 #167#) 
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COMMENT                                   "FFRT (F5 mutant)" "@N" 1 15.4714 1.74233 0 
COMMENT                                   -10 7.99245 1.17389 #169#) 
COMMENT                             #171=(CScratch 
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COMMENT                                   -10 8.14231 1.17389 #175#) 
COMMENT                             #177=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   "@N" 1 14.2643 3.48466 0 -10 6.16917 1.17389 
COMMENT                                   #177#) 
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COMMENT                                   1 10.7587 8.71165 0 -10 4.19603 1.17389 #181#)) 
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COMMENT                      #183=(CGroupWidget 
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COMMENT                            (CObjectList 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                             #185=(CLabel 
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COMMENT                                   -11.7333 -6.96932 0 -10 2.87229 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
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COMMENT                                   1 12.2267 6.96932 0 -10 4.79547 1.17389 #189#) 
COMMENT                             #191=(CScratch 
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COMMENT                                   1 13.126 5.22699 0 -10 4.79547 1.17389 #191#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #193=(CGroupWidget 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.2651 
COMMENT                                   3.48466 0 -10 10.4901 1.17389 #194#) 
COMMENT                             #196=(CWideArrow 
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COMMENT                             #197=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 -7.87604 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #196#) 
COMMENT                             #198=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    4.72762 1 0.082322) 0.8 1.8 0) 
COMMENT                             #199=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 17.0294 
COMMENT                                   -10.454 0 -10 14.9609 1.17389 #198#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -255 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #201=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #202=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #203=(AnlzFontColorItem 2890 2910 41975808) 
COMMENT         #204=(AnlzFontColorItem 3329 3348 41975808)) 1 
COMMENT        (CObList #205=(AnlzFontBackColorItem 3139 3809 33554687) 
COMMENT         #206=(AnlzFontBackColorItem 138 1810 33587328) 
COMMENT         #207=(AnlzFontBackColorItem 1811 3138 50266112))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     14011..17822
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(18921..20142)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     12560..13985
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     8919..12564
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     4965..8918
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    3867..3877
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    3874..4593
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4594..4629
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    4630..4702
                     /vntifkey="21"
                     /label=NLS
     misc_feature    4909..4959
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    3819..3866
                     /vntifkey="21"
                     /label=FFRT\(F5\mutant)
     misc_feature    4708..4755
                     /vntifkey="21"
                     /label=FFRT
     misc_feature    13992..14009
                     /vntifkey="21"
                     /label=I-Sce-I
     misc_feature    139..3810
                     /vntifkey="21"
                     /label=Ple47\(CX3CR1-D)
                     /note="1307"
     misc_feature    3140..3810
                     /vntifkey="21"
                     /label=Prom\Region
     misc_feature    139..1811
                     /vntifkey="21"
                     /label=Region\6
     misc_feature    1812..3139
                     /vntifkey="21"
                     /label=Region\7
     primer          2891..2911
                     /vntifkey="27"
                     /label=oEMS3045
     primer          3330..3349
                     /vntifkey="27"
                     /label=oEMS3037
BASE COUNT     5371 a      4612 c      4548 g      5617 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccgc ctgctggaac aaactgtctt ctccccatgc tcccactctc 
      181 ccctgctcct ccctccttca ataagcctcc tgtttttatt attttccccc tcgttttctt 
      241 cctattttct ttctttttag gtctcctctc agatctgccc tccagccaca gtccccttcc 
      301 aggaagggga aaggccacag aggggtaggc ggaggggaga aatggcattg ggcagggggg 
      361 gtgggtgatg ggtggggtgg ggcaccgtgt gcagccctaa atgtgagtga gggaaggccc 
      421 gaggctgatg cctccatatg cctctgcttc cccacactga accttcagga agtgtgggcc 
      481 aagcaggagt ctccctcaga accacagcca tcccccctac ttagaggacc tgtgcctgag 
      541 cttccaccct ggcttacttc ccacaggagg ctaggaagcc cttgctaatc agcatccagc 
      601 cttcacctgt cagctcctaa agagagaaga gagcagagaa gagagctgag aagagagcag 
      661 ctctaggagc ttccaacatt tgttcctcct gcccggcaca acaaagatct attttaattc 
      721 cctctgctgg ctagtcacta gcatagtgga atgacattaa tctcccttgt cagggggaag 
      781 ctgaggaagc caatacttct cctttgaggt ttgagttgca aaactcttcc tcaatctcca 
      841 gcattataaa tcctgttttt actttcacag ctatgtgcaa aatccaaata gcacctttct 
      901 aaaaatgaat agaagaagcc atgtcttatt ctgaaattaa gtttccgagg ctctaagagg 
      961 agaggactaa gaggggctat tccagcaggg acactgctac ccttgatggg acacgagagg 
     1021 agctgtgcac cctgaaggat gacctgaccc cctcccttct atttctaatt caaggaacat 
     1081 ggcccaggat gagacagtct aggaaattat gcacacccaa aagggtggca tcgtctgggg 
     1141 aaatgtcagc atcccagcac aaactgactc aaagaaagga actaccatct gtgtgtgtct 
     1201 gctgaataca ggcattgtgc taaagtaatc ttagcaatgt tgtctgcagc ccttcatggc 
     1261 ttttggtgat aggtacccag ccacagggca gtcctgaaag tctgagaaat tagtcacagc 
     1321 atcattcggg tcacaccatt gtggtggctg ttacctgcaa gaggctaaca aagaggtttt 
     1381 agggtatgac ttcaggttga ccaagtcaag tgcatgatct ttcctgagtt cattcctgga 
     1441 gagtgtctat gtgacgggtt cctgcctcag ccctcctggc agagagcctg tttggctgag 
     1501 ctgtcatggt atccactctg ggtgctctct tcctgctctc agtggaggag ggtggcagtt 
     1561 catcactgag gtgtgagtga gcctcagatg tttacgagga ggagtttcag gcgcatataa 
     1621 ctcaaagaaa acggctggtc tttcggaact tgctagcaga cctacctgcc acccagaact 
     1681 aaattcaatg aaactttatt tcatccctta taaagcatct ctcattttgt ggactgggat 
     1741 tgattccttt aaaagttgcc cttacaaata ccacatctgc aatggtccaa tcataagggt 
     1801 aaccactctt gaacatttac tttcttaaaa tcctcctgtg gtaaaattct tagtaagcag 
     1861 ttccacatga aggaagcaga caccagactg gaggctaaaa aagcacaaac cccacacgtc 
     1921 ctgtctatgt aagaacagat ctaacaaggc aggtctggtt ggagggaagg tctattttta 
     1981 tgagacgggg gaaaacagaa gtgtccatca ccagggctga gaaatgctgc aactctcagc 
     2041 aaccagctaa agataaccca ggccaggaac tctccaccca gactgaaggg gcctcagtgc 
     2101 tctgtgcatg caggataatt caagcttctc cagtgagcag ccatcccaag ctactggatc 
     2161 cttcagtttg ggaacactag cttaaccagc cttcctcagg aacttccttc tccctgaagg 
     2221 tggcaacaca ggaaaactcc ccatcagtcc ctcttaccca tacaccgcaa gccagttgtg 
     2281 atctctccct cctggcaagc aggatcctgg agttctaatc tgggctgggc ctagggtgag 
     2341 tcaagggaag cccctagggc acttgcatga tcctgagggt gagggcttcc ttacattttg 
     2401 cttcttagat gtttctctcg cctcactgga tggccagccc tggttctaat cccagatctg 
     2461 ctgttgacag gccatgtaca tgaggacaag tctcttgttc tctctggcct tcagtttact 
     2521 catccgagaa actgaagtgc tggagttaga tcagtgtttc ccaataactg atccatgaac 
     2581 ccaaaatgat ccttaatatt cagatacaca gaacctttac caaatgtcct cacttctgaa 
     2641 aatctaaaaa cttttctata agaacctaat gtatgactat agaatgtggc agatgtagtc 
     2701 agggtcctcc acaggactca ttcctcccta gagaaactgc accatcttct ctacagctct 
     2761 gtcagggctg tcccagccac tattgtaaca ttgctaactg ccctgacaaa aagtgattag 
     2821 actagaggta gaacccaagc ccaagatgaa ccaatctata gactggacag agacatgcag 
     2881 attattgctc tcacagtttg ggcactgcag actccgtagc caataatggt gggcaggtca 
     2941 gcagtcaggt cagactcaag gctgatgggc acagagaccc acgtgcaggc aaagaagctg 
     3001 attaaaagaa ggaggatgca gagagatgca gtgatgagta agtcttatgg tctatgaaag 
     3061 ttgagaaatt actttgctct tgagagaggg agggtgtccc cagctcctga ctttcccatc 
     3121 ctacgccaat atgaggcagg gcagagaggt gaggaaaaaa atatttaccg agcatctaca 
     3181 aggtgcaagg tactcactag atgccttcag taccaaagct tctcaaactt agtatgcata 
     3241 tcactcttct aagaatttca ttaaaatgca gattctaatt cagcagatat agggcagggc 
     3301 ttgaggtgct gtctttaata agctcccagt gcctgggact gcactttgag gagaagagct 
     3361 gtgtgtgccc cagtgtggtc cagtgagtac tctgggctcc ctctcatggg cagggaagct 
     3421 gagggcccca tgagctctcc cagcttcctg aaggctcccc attaatgaga gctgactgtg 
     3481 ctgtgctttg ctgactgcag ggcctactcc ctgcccccca cctccaggtt ggggtaagtg 
     3541 gcacctctct ccctccagct ccgcagtctt ccctgaggtt tagatcttcc aggtttataa 
     3601 agtcaggccc tcctgttggc agctggcctc caccctggag tatctgagct tgcctgtggc 
     3661 agcatctaaa gatagtctcc cttacaggaa acaagatact attggctaac tctgcaaata 
     3721 aaatgctctt agagggaagg aaagggaaat actcgtctct ggtaaagtct gagcaggaca 
     3781 gggtggctga ctggcagatc cagaggttcc ggcgcgccga agttcctatt ccgaagttcc 
     3841 tattcttcaa aaggtatagg aacttccgcc accatggtga gcaagggcga ggagctgttc 
     3901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
     3961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     4021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 
     4081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
     4141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
     4201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
     4261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 
     4321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 
     4381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 
     4441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 
     4501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
     4561 atcactctcg gcatggacga gctgtacaag ttatttctga actgctgccc gggctgctgc 
     4621 atggaaccgg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat 
     4681 ccaaaaaaga agagaaaggt aatataagaa gttcctattc cgaagttcct attctctaga 
     4741 aagtatagga acttcagcgg ccgcgactct agatcataat cagccatacc acatttgtag 
     4801 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 
     4861 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     4921 gcatcacaaa tttcacaaat aaagcatttt tttcactgcg aattctcgta aaactcttca 
     4981 tggcagtagt tattattctc tctctctctc tttttctttt ttcttgagac aggatatttc 
     5041 tctgttgccc aggctggagt gcagtggcac agtcttggct cactgcagcc tggacctcct 
     5101 gggctcaagc catcctccca cctcagcctc ccaagtagct ggggctacag gcacatggcc 
     5161 accaggccca gataattttt catttttgta gagactgagt ctcaccatgt tacccaggtt 
     5221 tattattctc attttttaga tgaagagact gaggtccaga gaagctcaat gacttgccta 
     5281 gttttacaaa tctcctgcca tcacataccc ctcagcgtcc ttaataagag ggaggccacc 
     5341 aactatgtgc tgggcactgt ggtggatgct ggagctatag ggttgagtat ataagaaatg 
     5401 gtgttgctgg agcaactgtt gcttgcttac ctgacctatc tgagaattaa ttagcagggg 
     5461 aacatatttt tgttttcaga ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt 
     5521 agtaataaca gtagttccca tttgctgact gtactgtcct aagtgcatat atatatacat 
     5581 acacacacgc atacctatac tcctctaata ctcaaaatga tcctgtttat gtattgttaa 
     5641 tatgctcatt ttatttttaa atttttattt atttttattt ttatttattt ttgagacgga 
     5701 gtctcattct gtcgcggagg ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc 
     5761 gcctcccggg ttcaagtgat tctcctgcct cagcctccgg actagctggg attacaggcg 
     5821 cccgcctcca cgcccagcta atttttgtat ttttagtaga gatggggttt cgccatgttg 
     5881 gccaggctgg tctcgtactc ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg 
     5941 ctgggattac aggcatgagc caccgcgccg ggctaatatg ctcattttag tgaggcaaaa 
     6001 atagaggctc agagtctgat ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg 
     6061 tgtaccctga tctgggtgac tctaggactc taggtctcaa ctgttacaac cagttaaggg 
     6121 tttggggaag cactgggcca agagtcagga aaatggaagc cacaggtagt gcaaggtctt 
     6181 gggaatggga cgtctggtcc aaggattcac gcgatgactg gaacccgaag agccggggcc 
     6241 cggtttacgg ccgccatgaa gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct 
     6301 gaataggaga ctgagttggg agggaaaggg gcttcgctgg gggagcctcg gcttcttctg 
     6361 ggagaaaatt cccacggcta cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc 
     6421 gcgggcgggg ccgggggcgg ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct 
     6481 gcttctcctc agcttcaggc ggctgcgacg agccctcagg cgaacctctc ggctttcccg 
     6541 cgcggcgccg cctcttgctg cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc 
     6601 tcctgagcag tcagcccgcg cgccggccgg ctccgttatg gcgacccgca gccctggcgt 
     6661 cgtggtgagc agctcggcct gccggccctg gccggttcag gcccacgcgg caggtggcgg 
     6721 ccgggccctg aggcgcggga tcgatccatg acaccccccc gccccccata tctcactata 
     6781 attcactgtc aaatggcttt tatgatttgg agtttgggga tacttttaaa gacagggtct 
     6841 tggtagctcc tagatggctt gggattcact tagtaaccct tgctggccta aaattcagga 
     6901 acttctacct catcttccca agtactgggt tagtagggat gtccagcata tttagcttgc 
     6961 tttaatgatt ttgctgtact tttttctgtg tgtacaggtg catgtatatt tataaaacta 
     7021 acataatata ttttaccccc aaagtaccat aaccctaatt aatgctatta aatgttttgt 
     7081 atgctgggct ctgggcatac taatataacc aatattatac aatgcaaaac aattttggaa 
     7141 tatcttctgt aaaattatct tattagtaag ttgtaaccat ctcagacttc taagtttgag 
     7201 tttgtaaagg atcagttttt tttggtgacc tacacacaca cacacacaca cacacacaca 
     7261 cacacacaca ggggggtgga ttctgagaat ttagagaatt tagatggtta ataccagttt 
     7321 ttatttttat tctgtgtgat atttatttat taagatttat ttatttatta catgtaggta 
     7381 cactgtatct gtcttcagac acaccagaag agggcatcag atctcgttac agatggttgt 
     7441 gagtcaccat gtggttgctg ggatttgaac tcaggacctc tggaagaaca gtcagtgctc 
     7501 ttaaccgttg agccatctca ccagccccgt gatattttat ttaacttact agaatatact 
     7561 gtgttaaaac atattttagg ggattgggga gatggcccat tctgtaaagt gtctgccatg 
     7621 tcatcttcag gacctgcgtt tgtaacccca gcatggagaa gacagagaca gggagacccc 
     7681 tgggcttcct ggcaagctag actagcaaat ttggttaact aggttcaatg agagactcag 
     7741 tctcaaaaca taagaagtga attttttaga cccaatttca atctctgaac tccatacgta 
     7801 tgcacatgta cctgcatata aatatatata cacacccata aacacatgca cacacaaaaa 
     7861 atggttcaaa agttcttaaa atgtataaga gtagaaagtg tagagccggg agtggtggcg 
     7921 cacgccttta atcccagcac ttgggagcag aggcaggtgg atttctgagt tcgaggccag 
     7981 cctggtctac agagtgagtt ccaggacagc cagagctata cagagaaatc ctgcctcaaa 
     8041 aaaccaaaaa taaagtgtat atactaatct ttcatgccta aaatatttat ttttgccaca 
     8101 tttttcatac ctctttaata tttttctttt tctctttgtg cttcaaagga aattttgtgt 
     8161 catgttttac ttttcattct tgactctatt cagcagtaag acgcagcatg gctttcttag 
     8221 agaagtataa tctattacaa aacggtaaca tttaactgct ctacataccc tcttgtagct 
     8281 aaattcctct gttaaactaa gacttattat ctaaacgtaa ttaaatgttg atatcttata 
     8341 atgctccact ttgaaacagc tgtctttctt ttgcagatta gcgatgatga accaggttat 
     8401 gacctagatt tgttttgtat acctaatcat tatgccgagg atttggaaaa agtgtttatt 
     8461 cctcatggac tgattatgga caggttagta agacctcgat tgaagttatt tttagagggt 
     8521 gtgtgtgtgg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag 
     8581 gagaggcatg tgtgatatat atgtgaagtg tattgcatgt acatggaatc cagagaagga 
     8641 cattaggtgt cctactctgt tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc 
     8701 tggggcatac atactgattt ggctaggctg cctggctagc aagacccagg gatctgcttg 
     8761 tctctgcccc ccagtgctga cgttacaagc aggtgcaacc acatctggtt tttatatggg 
     8821 tactggggga tctgaactta gggcctgatg cgtgcccaag tgctctcacc cgccaagcca 
     8881 cctctccagc caaagggttt tttttcttgt attaattcta acttagcaac tgttgagtag 
     8941 ttgatgtttg aatataagct aagccaactt attagaataa atgttttcta ttttttactt 
     9001 gtatgtgtga gtgcctgcat gtatgtatgt gaagcatatg tattccagga tgtcagaatt 
     9061 gtgatggatt ccctggaact ggagttacag accattgtga accactgtgt atgggtgctg 
     9121 ggaactgaac tttaggtcct ctgcaaaaac agtaaggatc ttaactgata aactatcttt 
     9181 ctagccctaa ggctttctac ttttaaagtt cgtttctctt tctctctctc tctctctctc 
     9241 tctctctctc tctccctctc cctccctcct cctcctcctc ctctttcccc cctctcgcct 
     9301 cctctttctc tctcccctcc ctctctctct ctcacacaca cacacagaca gagagaggag 
     9361 agagaaagga agccttacta attaattacc tagttttaga cataactagt gggaatccat 
     9421 agtaagtaaa tcaacattct tgaagagagg ttagaagaat tggctctcgt tgtccatcga 
     9481 ggctgtataa gattattctt ctgttttctc agcttaccat acatattttc tacgtagaca 
     9541 ataccaattc aaatacactg tacgtaaagt atatgcgcac acacacacac acacacacac 
     9601 acacacacac acaagttaca tattgtcata tagtactaaa atttatggac tttttcatat 
     9661 taatacatct agagctatca tgttttttat cttttaaaaa ttactttacg gggctacaga 
     9721 gatgtctcag cagttaggag cactgactgc tcttccagag gtcctgagtt caattcccag 
     9781 caaccacatg gtggctcaca accatctgta atgggatctg atccactctt atggtgtgtc 
     9841 tgaagacagt gacagtgtac tcatatacat gaaataaatc tttaaaaaat tactttacca 
     9901 tcagggtata tatatatata tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc 
     9961 tatttgagag ggtctcactg tttagctcag tctggcttgg aacgttctgt atagcccaga 
    10021 ctggcctcaa ctcagcagtc cccctgaaag gctctcatat gtggagatta caggtatgtg 
    10081 ctactgtgcc cagccctaca tgttactttg tttttgaaat agggtatcaa atagcttaga 
    10141 ctggcttcta agtcagtgtg tagctaagga tggtctgagc ttctgaacct gctacttcaa 
    10201 ctcctggtgt gccatcatgg ccagtttgca cagttgtgag agtagaactc aggacctcct 
    10261 atgtgttact agacatgttc tctaccagct gagctacatc ccaattcgcc tacatttact 
    10321 tttatttttt aaaagattta tttatgtata cagcactctg cctgcatgta catctgcatg 
    10381 ccagaagagg gcaccagatc tcatcataca tagttgtgag ccaccatgtg gttgctgggg 
    10441 attgaactca gggcctctgg aagagcagtc agtgctctta acctctgagc catctctgta 
    10501 gccccctaca tttattttaa agattatagg ctattctatt catagatgtg ccataactga 
    10561 tttaaccaga tcttcattat tttgtactta gtagagtata ttagagggaa tgaatcttaa 
    10621 atacacattt tgacacttgc tcagctttag acaagtaaaa atcactggtc aaggaaatgg 
    10681 tgcttagtgg atgcttctga actgtcaaat tgtcctccaa caagttacat cattttatac 
    10741 ttccaacgtt atgtgagagt tcatttttct ttaggctctt ctagtatttt atatttgaga 
    10801 aataaaattt aaccaaaatt tgccaatcta gtaagcttgt ttttatttgt atctttaaat 
    10861 tttatgatag atgttatagt gtactctcct ctcccctccc ctcccctctc ctcctcagca 
    10921 ggatcttgtt atgtagccct aagggatcta atacttactc tgtagtccag gctgtccttc 
    10981 agctcatgag acacaccatc atgcttggca tgtttcttgt tctgaaaggt atctggtttt 
    11041 aattttggtt gagctggtgt gttggtgtat atctgtaatc tcagcacagg caagtagaga 
    11101 caaggattat gattacagtc cagcctgggc tatttagagg gattctcttt aatctttcct 
    11161 catgccccaa aatcttacct ttggtatatg aaaaatagtc tccacttctg caaaatattg 
    11221 ctttatgaag taagaattaa ttcccttcat agagacaagg aatgtgtcct gtaaaagttt 
    11281 aatgtgtaag aagtatttgt tataaaagat aaatattcag aatcttcttt ttaattcctg 
    11341 attttatttc tataggactg aaagacttgc tcgagatgtc atgaaggaga tgggaggcca 
    11401 tcacattgtg gccctctgtg tgctcaaggg gggctataag ttctttgctg acctgctgga 
    11461 ttacattaaa gcactgaata gaaatagtga tagatccatt cctatgactg tagattttat 
    11521 cagactgaag agctactgtg taagtataat taacttataa ttaaaaaaat agggccattc 
    11581 tagttttatc tatatttttt ttaaacttgt gcaaactatg ctacataatc aacttacttg 
    11641 attatgtaat atatatagta atagttggaa ttactattat tacatattta tttttctgtg 
    11701 ctggggtaga acccagggaa ctaagtactg taataaccga gcttcattca tcctagtcat 
    11761 ttttaatttt tttttttttt tttgtttttt cgagacaagg tttctctgtg tagccctggc 
    11821 tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagaaatct gcctgcctct 
    11881 gcctcccagt gctgagatta atgacgtgca ccaccactgc ctggcatttt tttaaagttt 
    11941 taatttgaga ccctttctca ctaacttgct cacactatcc tcgaactcat tacgtgggct 
    12001 ggtctagaaa tttcagtcaa cttgcctcag cttctcaagt agctgggatt acaggcaggt 
    12061 gctaccactt ctggccatga tcattgttgt tgttgctatt attattatta ttatttcact 
    12121 gatacttcat atcacaacac agaatagtaa ctgtagttca tgaccaacaa aaaagttgta 
    12181 cagtttctct gttgtcttat tttgatctgg attttgattt gtttcatggc actgggcagg 
    12241 ctaatctcaa actgcatggc tcaaatagtc cttctgtctt aatctcccat gttctggaag 
    12301 tataggtatc actacaccta gctgtatttt catattttaa aaaaaacgcc taagaattgc 
    12361 tattgaatga ggcggagaga tggctcagca gtcacaagta cttgtagagg actggggttt 
    12421 ggttactagc acctcaaaga tggcttacaa ttatctgtag ctccaattcc agaggatctg 
    12481 tgtggtaaag ctgcatgtat atgggcatgc aggtaaaact gctaaacata taaaaatagc 
    12541 tcagtcttaa gagaattggt cgactttttc gccctttgac gttggagtcc acgttcttta 
    12601 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
    12661 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
    12721 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 
    12781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 
    12841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 
    12901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 
    12961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 
    13021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 
    13081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 
    13141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 
    13201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 
    13261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 
    13321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 
    13381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 
    13441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 
    13501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 
    13561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 
    13621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 
    13681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 
    13741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 
    13801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
    13861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
    13921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
    13981 accaggtcga gtagggataa cagggtaatg gatcctcgtt gggcacctgc actcatgtac 
    14041 gcaggcccac atatagatgt acacacgcat atcattcaaa atagaaatta tttatacaag 
    14101 tagtgcgttg gagagctggc tcaggagttg ttaatgcttg ctacccttgc aaaggaccca 
    14161 agttcaattt ccagcacctg tgttgggcat ctcacaacca catgtaaatc tagtttcagg 
    14221 ggatttcacc cctcttctga actcttcagg cacttatacc cacaaatgga catctccaca 
    14281 tagagacaca tacttttaaa aaagtaatct agggtctaga gaaatggtta tagaccacgg 
    14341 actgggatca gttcccagaa gccacatcct gtggctcaca attgcctgtg aatcaagttc 
    14401 tagatctggt ggcctcgtct agcctgcagt catcaacaaa cacatgcaca catccataca 
    14461 cacataattt aaaagaaagc gaaagtaacc tataatgcta aacaaagcac attctcagtg 
    14521 tagactgcag tggtgatgaa ccttgaaaat atattaatag aagccagaca cgtaagtcac 
    14581 aaatggtata actcaattta tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa 
    14641 aggcagatta ttggttgtca gtagctacag agatgggaca ttgaagatat taataggtat 
    14701 agggttgagg attcatggcc tttccctctt ccatgttagc atgtctattg ttgttaacct 
    14761 tattcaactc atgttagggc aatcatattg atgagactta atgagtatag cttgacattc 
    14821 ctagaagaca gagtctcaca tcagactctc tgatcctctg actcttagaa tctttcaaca 
    14881 ccctcttctg aaatgatctc tgagcctttg gttgtgactg aattatgaga aagaatttta 
    14941 tgttgaatga attttacttc agcattttta caaaactgaa gctggggatg gcgtttcatg 
    15001 cgtgcagtcc ctaccatttg cctataagcc tggaggaggc agaagtagaa ttaggctttt 
    15061 cagggacatc ttcagctgca tcatgagctt gaggacaact tgagctacat aagattccca 
    15121 tccccacccc cacacacaca cacacatcta gtatgtacct agtatgcagt cttttcatat 
    15181 gtttaaaatg tagaaaggta tatgtgaagt ctcccttttc cccacaactt agtccctcca 
    15241 aagtattgca tcagtttcct tgtacatgat tttatgcata tataagatac actatttctc 
    15301 tctctctctc tctccttccc tccttccctc caacctctgg gtatgagttg agggttatgt 
    15361 atgtttcaag ttgtaataag aattgccact caaacatttc atcccatttt aagttaagtt 
    15421 tgtctgattt tccactttag aagggcagcc atgatggctc atgcctataa tcccatccta 
    15481 gcacttgtaa gcctaataca taagactgga ggattgtcat cagcttgagg ctagcctggt 
    15541 ctacctaatg agttccagcc agccaagagt agaatataag accctgtctc caaaaaatgt 
    15601 accaaagggg ctggaggggt aactcagtgg ttaagaacag tagctgctct tccagaggac 
    15661 ctgggttcaa ttcccagcac ccacatggca gcttgcaact atctgtaact gtccagcttt 
    15721 gggatatccg acaccattat accaattaca taaaatgaaa aaattgtaaa atgtgctaaa 
    15781 atgagtgggg agggggagca tggtgtggtt ctgtgggctt gggattttgc catgaatcca 
    15841 tgactatatc tcagcaatat gtggattatt tctggaaacc tgtggggcat gggtgtatgg 
    15901 gaagagggga gagagagaga gcccgcatcc agccagagtt cctgtgctct gggcaggcgg 
    15961 acgtgggagg actgctggac gatttccact tggccctggg tgggcatctg gctgtgtgaa 
    16021 gccactgacc ccactcagcg gggggtagat aaggggcatt tcccatacca ggttctcaga 
    16081 ctccagcatt ccacgctgga tacagcagaa gagctgagaa cagaataggg gccccaggga 
    16141 gacactctcg tcccagagat aaagaaggca gagggagaga gattcctacg caggcgagag 
    16201 gccttagtct gggccttgat gaacagagac ggtctatggt tttagagctt tattgtagaa 
    16261 aggcaggggg aaagagagaa ggtagaaaga gagagaccag ccatggccaa gaggagagaa 
    16321 ggggggaaag gagagagaga agaaaggcta gagagtaaga aaggttaaag agacagaagt 
    16381 aaaagcttag agtaagagag agagagagag caagagggag agtaagaaaa gtaaaagctt 
    16441 aagagagcga ggaggggcca gcccctctaa tagtgggctt gttatcttgt tgttgctagg 
    16501 taactgtgaa gcgtctagcc tgaaggccag aagcttggga cattgtctat gtgactgaaa 
    16561 gccatgagcc tctactgtgg gggctgtgga ggtggtaact tggacaggag ccaggggttc 
    16621 caggagacat gagggaatgc cttctgtccc atgtaggtag gaattaccac caccggcttt 
    16681 caccgtagtt cactggagac tgtttgtaca tagcccattg ccccacagaa accaggcact 
    16741 cactgtttca tttgcataag aattagaagg ctttattttg gctgggattg gtgacttgcc 
    16801 ccagaaattc cagcttttgg gaagccgaat cctggagaac caggagttca aggtcatcct 
    16861 caggtacctc atttgttcaa gaccagcctg tgctaccatg agaccttgtc cctactcccc 
    16921 tcaaccccaa aacacgctgt tttcatttta taggttgtac taaccatgga ggatggtcat 
    16981 ttgcctttct cttgatttca gaagcgtagc gcgggctttg caccatgatc tagtgtttcc 
    17041 agataggctt gcctctccac agccaggctt atcctgcttc agtcccatgt ttggcaaggg 
    17101 caacatatga ctgaagaact tgcatagcct tatggtgtat catgactctt agccccagtt 
    17161 tataactgtc taacagctgg tggcttttgt gaaaaaggaa agcatcattt gaacttttaa 
    17221 cttttcagct gtttgttttg actttgcata aatactgaga ctagctagga aatgagcttt 
    17281 tgtttcattt gctaaaaatg attttttttt catgaggcaa tttcaagcaa tttgaattta 
    17341 gcaatctcta cttgtctgaa aagaagttat taattaaaaa aaaacaaaca aacaggaacc 
    17401 tattatgctg gctagtcaca tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca 
    17461 catgaaatta ttttctaatt ccccaaatta catttatgct aattagtatg tagtaaaaca 
    17521 ttttaattgt ttccaatgtt aaacattaca gaaataattt tgatgttttc ctaataaccc 
    17581 agcctttgat catgtttgca tttatttgat tgttgtcagt ttattaaata attaagtaag 
    17641 agcaacaaga taaaatgact tcatgagagt ccttttctgt aacttagaac gtcagtagtc 
    17701 ataggaactg cggtcgttcc cttacctttc agaagtaggc taaagagttg aacgcaaagg 
    17761 tggtttttcg aatcttcctg atccttaagc ccccactagg tttatattct atatttgata 
    17821 tcggattcga acccctcgag cttaggatcc ggaaccctta atataactaa gttattaggt 
    17881 ccctcgaaga ggttcactag tactggccat tgcggccgct tcgaggctgc ctcgcgcgtt 
    17941 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
    18001 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
    18061 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 
    18121 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
    18181 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 
    18241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
    18301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
    18361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
    18421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
    18481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
    18541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
    18601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
    18661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
    18721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
    18781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
    18841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
    18901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
    18961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
    19021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
    19081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
    19141 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
    19201 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
    19261 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
    19321 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
    19381 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
    19441 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 
    19501 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
    19561 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
    19621 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
    19681 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
    19741 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
    19801 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
    19861 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
    19921 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
    19981 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
    20041 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
    20101 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgg 
//
