LOCUS AM1956 10605 bp DNA circular 21-JAN-2008 SOURCE ORGANISM COMMENT In order to piece together this plasmid we used the pAM 505 sequence, the gfp mut2 orf sequence existing in the vector NTI database and the flanking sequences of the orf extending on both sides to pAM 505. For information on how the plasmid was constructed intially please refer to the AM strains database. Also the new sequencing result of the gfp orf using AMO 367 has revealed several substitutions (the peeks are clear and very well defined). I blasted the the gfp sequence from the vector and found that its is missing the first 6 nucleotides (which were a EcoRI site) GAATTC. Instead of the last 3 we have AGA. Also the C terminaland was 20 nucleotides shorter in the complete cds of gfp mut2 (from 10582-10602). COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|342017942| COMMENT VNTDBDATE|471872337| COMMENT LSOWNER| COMMENT VNTNAME|AM1956| COMMENT VNTAUTHORNAME|Golden Labs| COMMENT VNTOAUTHORNAME|Tel: (301) 216-0586| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList)) COMMENT #231=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 4063624 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 959394848 COMMENT (CStringList) 775168061 540094768 (CObList))) FEATURES Location/Qualifiers misc_feature 3107..9475 /vntifkey="21" /label=pDU1\sequences misc_marker 1574..2368 /vntifkey="22" /label=Km/Nm\R terminator 9505..9681 /vntifkey="43" /label=Terminator /note="I am sure the terminator starts at EcoRI and ends before PstI, but probably the size of the terminator is not accurate." CDS 9859..10582 /vntifkey="4" /label=gfp\mut2 primer_bind 9748..9767 /vntifkey="28" /label=AMO\367 /note="6 bp mismatch" primer_bind complement(220..236) /vntifkey="28" /label=AMO\369 /note="exact match" primer complement(9868..9886) /vntifkey="27" /label=AMO624 primer complement(9945..9970) /vntifkey="27" /label=AMO1396 misc_feature 9859..9861 /vntifkey="21" /label=ATG BASE COUNT 2761 a 2687 c 2450 g 2707 t ORIGIN 1 ggggatcctc taggatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 61 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 481 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 901 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 961 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 1021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 1081 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 1141 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc 1201 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 1261 ctggcttctg ataaagcggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc 1321 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg 1381 cacctgtggc gccggtgatg ccggtgggcg aagaactcca gcatgagatc cccgcgctgg 1441 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg 1501 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac 1561 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat 1621 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt 1681 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc 1741 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat 1801 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca 1861 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg 1921 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg 1981 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg 2041 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt 2101 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca 2161 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct 2221 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc 2281 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac 2341 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag 2401 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc 2461 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc 2521 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct 2581 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc 2641 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg 2701 agtgcttgcg gcagcgtgaa gctttctctg agctgtaaca gcctgaccgc aacaaacgag 2761 aggatcgaga ccatccgctc cagattatcc ggctcctcca tgcgttgcct ctcggctcct 2821 gctccggttt tccatgcctt atggaactcc tcgatccgcc agcgatgggt ataaatgtcg 2881 atgacgcgca aggcttgggc tagcgactcg accggttcgc cggtcagcaa caaccatttc 2941 aacggggtct cacccttggg cgggttaatc tcctcggcca gcaccgcgtt gagcgtgata 3001 ttcccctgtt ttagcgtgat gcgcccactg cgcatagaaa ttgcatcaac gcatatagcg 3061 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatct agacttatat 3121 agacactaat atagacaata gtttatactg ctatctatac aagtatagac attatctaat 3181 catggcagac aaaactctag ccacttttcg tattgactcc gaagaatggg agtcttttaa 3241 aaaccttgct agttctgaaa gttccaacgc ctcagcactg ttaacagaat ttgttcgttg 3301 gtatttggca ggtaacaggt ttaatactcc cacttctcac actcccaccc atctagacac 3361 atccctcgaa cagcgtatag acaatattga acaacgtcta gataaagtca caactaataa 3421 tctagacaat atagatgaat ttatagacaa gcgtatagaa gataatctag caacacgtct 3481 agacaaactt caatcgcaac tggaggaact gcggggaaaa tcgaaagccc ggtagttcag 3541 gcagaaggac aagctaccgg gcaagacaga aagaatatag acaatagtat agacaatcta 3601 gacaaattgg aggcaacccg cgatcgcacc ctcaataagc taaaaatggg taggcagtca 3661 gccgccggga aagccatcga cgcgtttatc aaagagttgc tttcttcagg agacaacata 3721 agctgaagtt atcaaaattc tgtccttacg tcgaaagcct gattttaccg tgcaacgatt 3781 gataagcttg gctaaactag cactggcttt caacagaaag catacgaaga atcaatagat 3841 atagccacca attccacaaa atgcagataa cgtgtagagt attggaatgc ttaatctgta 3901 agggttatga aggttaacgg caacggacga gccaaaatac tcacctccga cgaactcagg 3961 cgactgttta gcgacggatt caccacaccg cgcgatcgcg ttttgtttgg catctgtcta 4021 ttcaccggtt gccgcgttag tgaagctcta gcactccaaa caacggacat taaaggcgaa 4081 acactaacct ttaggaagtc taccaccaaa gggaaactca aaacccgcgt ggttgacatc 4141 cagccaggac tagccgcact catggctgac tatcacccca aaccgggaac cctgttccct 4201 ggcatgaggg gagtcagcga taggctcacg cgatacgcgg cggataaaat cttgcgcgat 4261 gcagccaaaa gaatcgggct agaaggcatc agtacccaca gtttccgccg tactgccctc 4321 aaccaaatgt ctagcgccgg tatcccgttg cgacacattc aagagatatc cggtcacaat 4381 gaccttggca cactgcaacg ctatcttgaa gttacacccg aacagcgacg caaagctgta 4441 tccgtgattg gcttctaatg tacgccaacg ctgtttagac ccctatgggt gctaaaaaaa 4501 gacgcagcct aaacacacgc tctacacttg aggatacttt taaagtatcc atcggttcta 4561 gaactctgca cacgttccgg actttggaaa cgttatacct ttccctgtgt tgcagaatgc 4621 tgcaatattt cttcgacaag ttaacttgtg actggtttaa tattttctca aattgcccca 4681 aaacaacacg cctaaatcct tagacgtttc tgtggaaacc tattaggttt ttatcgccgt 4741 tgttttagtg gtaaacccaa agggtttgta tattcttgta tgaagttcga ctctgagggt 4801 taagaagaat ggctcgccga attttttaca agtggaaacc gattaaaggt taagggtcaa 4861 tcgggacgat gaatattttc taattgtgac cttctccatc taataagctt tctttggggt 4921 taaggtcgaa gaaagtacta cgcatgatct gcatacgatc tctattgcca aaaagccgcg 4981 accctatagg ctctcggtca tgctgcacta gttcgtgtcg atcactatac tggttgccgc 5041 agcatttcac gctaaaaaaa aattcttaaa aatgtccttc atatctcgcc agagtggcaa 5101 cctattacaa aacggttgcc tacccgaccg gctcgatttt cgctgaagtg gcactgtgac 5161 agtttgaaat ggtacttccg ccgtgctgct gacatcgttg ttagggtgaa ttgttcgcgg 5221 tagatgttgc accgattcat gaacaccttg tcacccactt tgaataatcg accgtcaaat 5281 tcagtcgcgt caatttggta agtgttgggc tgtctctttt tggctccagg ggcaatgcca 5341 tcagaaaaca caaccgcgtc acccataact tgataaccga tatcagtttt ggttccagtg 5401 aaagcccaaa attcagacgc gtcattattc cgagcgtgcc ggagttgatt gtactcaatt 5461 ttggcttggc aaagttgacg gcgattcatg cccagctgct tttgatgtcg tcgcactgtg 5521 cgcttgtgaa tacccaactc acagctgaca gctttttgag atgtaccata gtggatgaaa 5581 ctttttgaga cgaatatccg cgacgaacta atgtgaagta cacaaggtac ttccccctct 5641 ggcgatttaa gagaggattg ccttgtgtcc ttcactagct cgttcgggtg tggcgctcca 5701 aaaagttttc tgtactctgg tttaagttgt ctgttggccg catagcggct cttttgttga 5761 aagctttgtg tgactatgcc agtggtcagt gagcgtaaat cgcttaacac ttggactaaa 5821 ggcactactg caacatcacc ccatcttttt aaatttaggt tgtaacaaac ttgaaacata 5881 ccgcccaagt agacggttat cattcctgct ttaattttgt agcggcggaa tgctcctatt 5941 ttttttccat cctgtaacca acggtaaaca gacttatcac tacaatctaa gaacgtctgt 6001 actacaggca atggcaatgt taaatgacca gacccatcct tatcaagcgc tcgacacaaa 6061 taccacaacc gcgcacaagg ttctcgacca atgcgagtgt gtaccctgac cgtgtaagtg 6121 ccaagaatta tttcagtttg tagttccctt gtaagcaggg ttagtgatac atttgtattt 6181 aagctttctg ggctgatcat ttggaaatgt ctcagtccag tacctattga atgttatttg 6241 cttaacctga agctaaataa aacttgttaa ctacacccat taattgataa attcaaagca 6301 cgttttttct gtttggtgtt tggtgtggta acaattctgt gtatgtgtgt tttatttagc 6361 ttcggttaag tagcataaca acctccaagc actgaacttt ttttaatagg taatttaaac 6421 tttgcctatc ggcaaaattt tcaatcaatt gtacgccaaa gtgttgcatg atcaacgttt 6481 gacttatttt tgtatttact aaatactgaa tttcgccgtg acgcttttta cagatggaaa 6541 ttcacggcaa aatgtttttt gctaactttg ctatgtaaaa caagaaactt ggcactcggt 6601 tattactaaa taaactggta aaaaataacc attagaacca aaaagaacga aaaccagtac 6661 acccttgcca gttttcaagc ttttgctatg acgactctaa taatcgggtt taacaccatt 6721 ccgctttgag aaaattatcc ttgtacagca agtaacagtc aatgctaaac gcaccgctac 6781 aaatccttaa gtttttccag tagcgattta ccttcttggt aactgcccgc cttgatagcc 6841 cagaatgtct ttaatcactg taactttctg aaaaacgcct gcgttccaga caggcttttg 6901 ccacttttgc tagggtttca tctcttggat ctggtaggga tgaaacgggc tgtaatgctt 6961 gttctgaggt cggttgacgc cgttgtggac gtgggtgaaa actggttaca gactgaaccg 7021 gggcataacc attatgtaac tgctttacat cttggtaact gacacggcat atcatccacc 7081 atgcagcgat atttccccga gctttaacca ctccacaagg gcaaggtctt ttaaggactt 7141 ggcgtggcta actgcaaact tacccaggcg taacatccta aacacttacg gacaccgcct 7201 tcaccctcga tacctaaggt cttgacatta tcatcttgag tcagcccaat aacaaaacgc 7261 ttgggcttgc ggccgcgcct ggcgtgtttg atgagccatt cggttgctat ctcgacttca 7321 tctctcagca gtggcagttc ttcagcaatt aaaacgcttt cttttcctgc tagtgcctta 7381 tccccagact caccccgtag ctcaatccgg cgctgcaatt cctccaggtc agcagccatg 7441 cccgactgta tagcctcaaa gtcaccacgg cggccaatga catttaaccc cgtccactcg 7501 tccggtgcag cgtcagcgtc atagactgtc acctcacccc cgacttgata agcaagccat 7561 tgggctatgg tgcttttgcc agttcccgta tccccaacta ttaaacagtg cttaccagac 7621 agagcttgca tcaagtcggt gatgattccc tctggttcga ccgcaagggt gacggcggta 7681 gtgtcaatga tagccgcgcc gtaagtgcca gcatagggca attggtcgta aactttgacc 7741 aagttgtata cagactgtct acaccacttc accactgtta acgctgtttg caaagcgtaa 7801 gacgtggcat caaataaaaa tatgctggca ctaaaagtta atcgccccaa tccccacagt 7861 aaaaacctgc ctagctgttg acgactaggc aagtgcattt caatccagtc atttgccata 7921 aatcaccccg tctttaaagc cttgcagttg agcgcgacag gtatttaact gtgcttgtaa 7981 ctctgtttgc tggttttgat accacagact gacggcggcg gccgccagtc ctaaaaatag 8041 aaactggcga tcgctcatta ttgacttact ccctgttgat tagcgtggta gtgagtcata 8101 gccgcattga ccgcttcttg ggcttggggt gttctgccaa gattgggttt tgtagggtca 8161 tcgttggcta cgactaagga cgcttgttcg gctatcgctt gcgggacacc aactttagtt 8221 aactctgtca aggatacttg gtaaagtcgc tcgttcatta gccgattctc cggtacataa 8281 aactgttgct ggcagtccct tcattggcga cgagttcttc agccggagta tcagcgataa 8341 tgtcagccca gccggtgaca ttattattaa taatgttttg ttcggcaatt gcacccaagc 8401 caggacgcgc cgtttcaaac tcagagatga cttgctgctc tttctcggtg agtggtctat 8461 ctgtcatgat aattatgtcc ttcattatgt aggcgattcc agtgggtgtt tacgaggcag 8521 tccacaggaa tcagtgcgat tcacctttaa ggtgaatcgt catcaaaaaa tcactcggta 8581 gcaacgaccc gaaccgacca ggattgattt cccggttctc agttcgcagg cttttgagcg 8641 cgtcaccttg accattgggt aactgccatc agccgataag ctaaacgggc tgtatagcgg 8701 taaagcatcc cacacagtcg ggctggcatc aactttgcag gaatagctca cgtcactcat 8761 ctcactcgcg cctgggttgg atggcagcga aggcagatta cgacgcagtt ttttactggc 8821 acttttaccc gcattaaaaa cgggtacagt gccattgttg acggtctgta cttcggtcat 8881 atactcggtg tacacttaat acactctata ctattactgc cgattagtac atttgtcaat 8941 cactctttgc acaaggtgta tgatatggac tcaggagtac accaaacgtc atgccaacca 9001 ataaagggag aatagcagtc actctagaag ctgaaattta ccaatggatt gctaaccgag 9061 cgtctgagga aggaagaccg ttggctaatc ttgccgcttt cttactcaca cgagttgtta 9121 aagaacaaat ggaacaagaa gccaaggaca accaagacaa gcagggggca gcatgagcga 9181 agacagacta gccagaatag aagctgcgtt agacagccaa gttgcagtga atgccgacct 9241 ccgcacatcg gttacagaac tccgcgcaac cgcagaagca ttgttgcaaa cagttcaaat 9301 ccatcagcag aactttgaaa ttcttaccgc taggcaatta caaaccgaag cacggcttga 9361 tgagtaccaa cgtaccacta gcgcggcact cgacagaatt ggcgcggtct tagactacct 9421 cgttaggcag caaaacggtt gaggtgaggg atgagcgatg actatctaga cggatatccc 9481 gcaagaggcc ctttcgtctt caagaattcc cgtttgactg gcgatgctgc tactgaaact 9541 aacaactaca tcgactacgc aattaacgcc ctcagctaat tttgcttagt ctaggcccgg 9601 atgggtaagt ggttttcagc ttaagtgttg ggttctactt acttctccgg gtcttgctct 9661 atctaaaaac attggtttaa caaggagtat taggcaaatg ccagttactg tcgtgcctct 9721 cgcttgggaa ccgctgcgtt tgaccaatca cccgtcgaac tgcgcgctaa ctattctcga 9781 cctgcaggtc gactgctaga ggcatcaatt cgagctcggt acccggggat cctctagatt 9841 taagaaggag atatacatat gagtaaagga gaagaacttt tcactggagt tgtcccaatt 9901 cttgttgaat tagatggcga tgttaatggg caaaaattct ctgtcagtgg agagggtgaa 9961 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg gaagctacct 10021 gttccatggc caacacttgt cactactttc gcgtatggtc ttcaatgctt tgcgagatac 10081 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 10141 gaaagaacta tattttacaa agatgacggg aactacaaga cacgtgctga agtcaagttt 10201 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 10261 aacattcttg gacacaaaat ggaatacaac tataactcac ataatgtata catcatggca 10321 gacaaaccaa agaatggaat caaagttaac ttcaaaatta gacacaacat taaagatgga 10381 agcgttcaat tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 10441 ttaccagaca accattacct gtccacacaa tctgcccttt ccaaagatcc caacgaaaag 10501 agagatcaca tgatccttct tgagtttgta acagctgctg ggattacaca tggcatggat 10561 gaactataca aataagaatt cagacctgca ggcatgcaag ctggg //