Name                type                location            
------------------------------------------------------             
M13 fwd             primer_bind         53..69              
T7 promoter         promoter            76..94              
Peft-3              promoter            130..738            
3xFLAG              CDS                 753..818            
SV40 NLS            CDS                 825..845            
Cas9                CDS                 870..1910,1962..2948,3000..4061,4113..5123
egl-13 NLS          CDS                 5130..5204          
tbb-2 UTR           3'UTR               5214..5589          
T3 promoter         promoter            rev:5645..5663      
M13 rev             primer_bind         rev:5684..5700      
lac operator        protein_bind        5708..5724          
lac promoter        promoter            rev:5732..5762      
ori                 rep_origin          rev:6086..6674      
beta-lactamase      CDS                 rev:6845..7705      
bla                 promoter            rev:7706..7810      
f1 ori              rep_origin          rev:7836..8291