LOCUS       pICE                    5044 bp    DNA     circular     15-DEC-2011
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    1221..1823
                     /vntifkey="21"
                     /label=PuroR
     rep_origin      complement(2467..3137)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        complement(4143..4241)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(3282..4142)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     misc_feature    4318..5042
                     /vntifkey="21"
                     /label=CMVTET
     misc_feature    4904..4943
                     /vntifkey="21"
                     /label=TetO
     polyA_site      131..355
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     rep_origin      401..829
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        834..1203
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    1953..2083
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
BASE COUNT     1145 a      1390 c      1290 g      1219 t 
ORIGIN
        1 aagcttgcca ccatggtgct gcacgagtac gtgaacgccg ccggcatcac agacaaatgc 
       61 ggccgcgaat tcggatccgg taccctcgag acgcgtctgc agtctagata aacccgctga 
      121 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 
      181 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 
      241 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 
      301 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 
      361 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 
      421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 
      481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 
      541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 
      601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 
      661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 
      721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 
      781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 
     1201 tcggatctga tcagcacgtg accatgaccg agtacaagcc cacggtgcgc ctcgccaccc 
     1261 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 
     1321 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 
     1381 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 
     1441 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 
     1501 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 
     1561 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 
     1621 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 
     1681 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 
     1741 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 
     1801 tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt cgattccacc gccgccttct 
     1861 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     1921 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 
     1981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctagcc gtcgacctct 
     2101 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     2161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     2221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     2281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     2341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     2401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     2461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     2521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     2641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     2701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     2761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     2821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     2881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     2941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     3001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     3061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     3121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     3181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     3241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     3301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     3361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     3421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     3481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     3541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     3601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     3661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     3721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     3781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     3841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     3901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     3961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     4021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     4081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     4141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     4201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     4261 aaagtgccac ctgacgtcga cggatcggga gatcctgtac gggccagata tccgcgttga 
     4321 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     4381 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     4441 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     4501 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     4561 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     4621 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 
     4681 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 
     4741 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 
     4801 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 
     4861 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga 
     4921 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc 
     4981 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc 
     5041 cggc 
//
