LOCUS Ple153\(pEMS1238 19527 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' GCGTATCACGAGGCCCTTTC - 3' Tm=56.0 Deg C Antisense primer: oEMS2968 - 5' AAAGATCCCCTGAGGTGCAGC - 3 Tm=56.9 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 267 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543240953| COMMENT VNTDBDATE|544979891| COMMENT LSOWNER| COMMENT VNTNAME|Ple153 (pEMS1238)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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33619967))) (CObList) 0 COMMENT (CStringList) 0 1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 13390..17201 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18300..19521) /vntifkey="22" /label=AmpR misc_marker 11939..13364 /vntifkey="22" /label=AmpR misc_recomb 8298..11943 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4344..8297 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 3246..3256 /vntifkey="21" /label=Kozak\consensus misc_feature 3253..3972 /vntifkey="21" /label=EGFP misc_feature 3973..4008 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4009..4081 /vntifkey="21" /label=NLS misc_feature 4288..4338 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3198..3245 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 4087..4134 /vntifkey="21" /label=FFRT misc_feature 13371..13388 /vntifkey="21" /label=I-Sce-I misc_feature 133..3183 /vntifkey="21" /label=Ple153\(OXT-B) /note="1307" primer 279..299 /vntifkey="27" /label=oEMS2968 primer 54..73 /vntifkey="27" /label=oEMS2364 misc_feature 2716..3183 /vntifkey="21" /label=Prom\Region misc_feature 133..763 /vntifkey="21" /label=Region\3 misc_feature 764..2715 /vntifkey="21" /label=Region\4 BASE COUNT 5100 a 4522 c 4598 g 5307 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta gggtcccggc tacccgaggg gacagagcgg gtgctgttct ggctgccacc 181 accctggagg ggctggaaga gggaggggca caaaggctct gaggacgtgg aaacagcccc 241 agcttcccgc tcccagcctg ggctgaggcc tacagcaggc tgcacctcag gggatctttc 301 aaaattcaaa aagacgagga tgagggagca aatacagaca agggtctgag cgggatgacc 361 acaggaagcg ggcagtgcac actgaatggc ttcctgaccc ctgaaaggag gtttgagcca 421 cacttagaag ttgagggagc ggagcgggcc tggggggagg gggccctggg gagcagctga 481 agggccacca tgtttccaag gagctggccc ccaggaggcc actggcacgc tcagaagagg 541 agcccaagct gccgggactg ggcttgaatg ccagcatgac cgtccctggt gtccccaggg 601 ctgggtctga cccagcagct ctactcctgc ctgtgcaata aaacatgtca gggctgggga 661 ccccacaagc ggacagggga tcttgagagc ccagggccac agaaatgaca tgaacaaaga 721 gaagctctta acctctatct cctatcggtg aagcaaaagt atcgctgtgt ccttaatgta 781 gatgctgaaa aataaataaa taaaaataaa aattaaaaag agattgccga tgttaccatt 841 tttatgaaac agcagatgtg gaggcttgga cactgctttg ccccttgtgg tgggtttgcc 901 gcagcccatt tgccctgtag agactgagca gacaagggca gacctctcct tgctaaatgg 961 ctctgtagct gggccagggg actcaaggag agggagaaag agtgaaagac tgtacaggga 1021 ggtggacatt tgggcaaatt gggggtgggc agacattcgt caccccttcc aagaagtagt 1081 agcctgattg ttttgacaaa gacaagtgga gggaaaggag tctctgaatc tgtatttagg 1141 aagaggaagt tcctggacct cattctaaaa ctgtccccaa gtccagacac taacactttg 1201 gattgaattc agtgcagcca agccacctgc ctcttcctat ttcccagtct ggaagagtta 1261 ataaattcct attaggtttg gtcctgctct agccccagcc agatggggga tggccctgag 1321 tggacaacat gaaacaggag caggaggtca tggcagtgac ccaagcctgc atggtccctg 1381 tccccactgg ctagacccag tcgtgccggg cactgcctgg cagaggagcc cagaaccagg 1441 tcaggggtga gagccaaggg gagccctggg agtgtggaag agagcctgac cttgacatgt 1501 gcctggactc tgcccagggc tgctgaggct gggctgcttg agtcaccagg cttggccgta 1561 agcctgggca ttggggtcag ggcccaggtg gacttccctc ctcctgcact ccctcctgac 1621 ctcagagttt gcggaggatc ccgaaggaga tgcagcctct tgtggctcca agacctggag 1681 caggaagccc aaggagagga tgcagagaaa agaggaggcc aagagagaga aggatgggag 1741 gtggcagagg gggtgagggg acagaagggg aggggtggag gggccaagga gagaggcaag 1801 agggggccca ggagctggca gaacagggag gcgagagcag agtgagagac aaaggagaga 1861 aaggggcaga tgggaccaga aaggcctcag gttcaagaag ggggtgagag aacccaaggg 1921 atgcagagga agaggccgag cactgctgga cgtcttttcg ccaaaggggc tcatgtcaaa 1981 gggcggaagg tcttcatgtc tgggagctaa tgtgagtgca ctcctgcttg tgtgagcctg 2041 tacatacatg ctcctgccca tcctcgtgtc ggctgtgtct gtgtttctct gggtgtctgt 2101 ggctctgaat gtggggctca gggtctctgt ggttgtggtc aagggttgcg gacacgttct 2161 tagcagttat tttcttgtct gtttctgtcc aagcgtgctt ggggttgtgt ggggctgtat 2221 ctgttagggg tcccccactc tggctgaggg gccctaatgg tgggtgcact ggacagacag 2281 tgatgtcccc accgccacta cacacgagca ctgccttggg tgaatcagac attgcactgg 2341 gaatcccggg accagggtgg gtccctgagc ggtttgtgtt tcagtctcct gggatgaaaa 2401 tggggacaga atggtcaccc ctcggcctgt cccctctgcc tctctcactc ttctctccgt 2461 cactgtcaat gtctctgcat ctctctccaa cattctgctg tctctgtctc tgggtctctt 2521 gctgtcttgt cctccaggcc tttccctctc tgcctttact tctttccctc tgccggagga 2581 tcctcaaccc accccctcca aggccactct cggcctctgg tctccccctc ccctgcctcc 2641 caattcgccc tgtccctcgg tctagcgaca cctctgcggg tcccagatcg cttcctctac 2701 gggacctgtt gcttcgaaga ggaaatgttc ctaaaagagg ggacaatgat ccacatgcgt 2761 ggtgggccaa gagggtgggc ggatcctgcc agagcctcct cccacctgga ggggtcccag 2821 cgtccacctt ccctgcccca gcccccctcc tcgaggtact gggaggctgg ataaagtctt 2881 cggctgggcc acaccccacc ccaaattctc cctgtcccac cctagtggcc caggccaccc 2941 cggcctgctc ccttccgcaa ggcacctcac cttctgtgcc cagaccatta gccaacgcgg 3001 tgaccttgac cccggcccag gccctgctaa tgaagaggaa agcccgtacg cactcggcct 3061 gacccacggc gaccctctgt gaccaatcat actaccaacc tcttaaacag agctccaccg 3121 acgcaatgcc caggcataaa aaggccaggc cggagagacc gccaccagtc acggaccctg 3181 gacacgcgtg gcgcgccgaa gttcctattc cgaagttcct attcttcaaa aggtatagga 3241 acttccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 3301 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 3361 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 3421 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 3481 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 3541 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 3601 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 3661 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 3721 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 3781 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 3841 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 3901 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 3961 ctgtacaagt tatttctgaa ctgctgcccg ggctgctgca tggaaccgga tccaaaaaag 4021 aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta 4081 atataagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagcggc 4141 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 4201 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 4261 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 4321 aagcattttt ttcactgcga attctcgtaa aactcttcat ggcagtagtt attattctct 4381 ctctctctct ttttcttttt tcttgagaca ggatatttct ctgttgccca ggctggagtg 4441 cagtggcaca gtcttggctc actgcagcct ggacctcctg ggctcaagcc atcctcccac 4501 ctcagcctcc caagtagctg gggctacagg cacatggcca ccaggcccag ataatttttc 4561 atttttgtag agactgagtc tcaccatgtt acccaggttt attattctca ttttttagat 4621 gaagagactg aggtccagag aagctcaatg acttgcctag ttttacaaat ctcctgccat 4681 cacatacccc tcagcgtcct taataagagg gaggccacca actatgtgct gggcactgtg 4741 gtggatgctg gagctatagg gttgagtata taagaaatgg tgttgctgga gcaactgttg 4801 cttgcttacc tgacctatct gagaattaat tagcagggga acatattttt gttttcagat 4861 tcaatataag aacttgtgtg ggcaaaaata aagatcagta gtaataacag tagttcccat 4921 ttgctgactg tactgtccta agtgcatata tatatacata cacacacgca tacctatact 4981 cctctaatac tcaaaatgat cctgtttatg tattgttaat atgctcattt tatttttaaa 5041 tttttattta tttttatttt tatttatttt tgagacggag tctcattctg tcgcggaggc 5101 tgaagtgcag tggtgcgatc tcagctcagt gcgacctccg cctcccgggt tcaagtgatt 5161 ctcctgcctc agcctccgga ctagctggga ttacaggcgc ccgcctccac gcccagctaa 5221 tttttgtatt tttagtagag atggggtttc gccatgttgg ccaggctggt ctcgtactcc 5281 tgaccttgag tgatccacct gcctcggcct cccaaagtgc tgggattaca ggcatgagcc 5341 accgcgccgg gctaatatgc tcattttagt gaggcaaaaa tagaggctca gagtctgatt 5401 tgtacaaaac tacagagcag ttaagtgtcc tctcagatgt gtaccctgat ctgggtgact 5461 ctaggactct aggtctcaac tgttacaacc agttaagggt ttggggaagc actgggccaa 5521 gagtcaggaa aatggaagcc acaggtagtg caaggtcttg ggaatgggac gtctggtcca 5581 aggattcacg cgatgactgg aacccgaaga gccggggccc ggtttacggc cgccatgaag 5641 caacgcgcgc cggtaggttt gggaatcagg gagccctctg aataggagac tgagttggga 5701 gggaaagggg cttcgctggg ggagcctcgg cttcttctgg gagaaaattc ccacggctac 5761 ctagtgagcc tgcaaactgg taggcgccgg cgtaggcgcg cgggcggggc cgggggcggg 5821 gcctgcgggg cgtggcgggg cgggcagagg gcggggcctg cttctcctca gcttcaggcg 5881 gctgcgacga gccctcaggc gaacctctcg gctttcccgc gcggcgccgc ctcttgctgc 5941 gcctccgcct cctcctctgc tccgccaccg gcttcctcct cctgagcagt cagcccgcgc 6001 gccggccggc tccgttatgg cgacccgcag ccctggcgtc gtggtgagca gctcggcctg 6061 ccggccctgg ccggttcagg cccacgcggc aggtggcggc cgggccctga ggcgcgggat 6121 cgatccatga cacccccccg ccccccatat ctcactataa ttcactgtca aatggctttt 6181 atgatttgga gtttggggat acttttaaag acagggtctt ggtagctcct agatggcttg 6241 ggattcactt agtaaccctt gctggcctaa aattcaggaa cttctacctc atcttcccaa 6301 gtactgggtt agtagggatg tccagcatat ttagcttgct ttaatgattt tgctgtactt 6361 ttttctgtgt gtacaggtgc atgtatattt ataaaactaa cataatatat tttaccccca 6421 aagtaccata accctaatta atgctattaa atgttttgta tgctgggctc tgggcatact 6481 aatataacca atattataca atgcaaaaca attttggaat atcttctgta aaattatctt 6541 attagtaagt tgtaaccatc tcagacttct aagtttgagt ttgtaaagga tcagtttttt 6601 ttggtgacct acacacacac acacacacac acacacacac acacacacag gggggtggat 6661 tctgagaatt tagagaattt agatggttaa taccagtttt tatttttatt ctgtgtgata 6721 tttatttatt aagatttatt tatttattac atgtaggtac actgtatctg tcttcagaca 6781 caccagaaga gggcatcaga tctcgttaca gatggttgtg agtcaccatg tggttgctgg 6841 gatttgaact caggacctct ggaagaacag tcagtgctct taaccgttga gccatctcac 6901 cagccccgtg atattttatt taacttacta gaatatactg tgttaaaaca tattttaggg 6961 gattggggag atggcccatt ctgtaaagtg tctgccatgt catcttcagg acctgcgttt 7021 gtaaccccag catggagaag acagagacag ggagacccct gggcttcctg gcaagctaga 7081 ctagcaaatt tggttaacta ggttcaatga gagactcagt ctcaaaacat aagaagtgaa 7141 ttttttagac ccaatttcaa tctctgaact ccatacgtat gcacatgtac ctgcatataa 7201 atatatatac acacccataa acacatgcac acacaaaaaa tggttcaaaa gttcttaaaa 7261 tgtataagag tagaaagtgt agagccggga gtggtggcgc acgcctttaa tcccagcact 7321 tgggagcaga ggcaggtgga tttctgagtt cgaggccagc ctggtctaca gagtgagttc 7381 caggacagcc agagctatac agagaaatcc tgcctcaaaa aaccaaaaat aaagtgtata 7441 tactaatctt tcatgcctaa aatatttatt tttgccacat ttttcatacc tctttaatat 7501 ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc atgttttact tttcattctt 7561 gactctattc agcagtaaga cgcagcatgg ctttcttaga gaagtataat ctattacaaa 7621 acggtaacat ttaactgctc tacataccct cttgtagcta aattcctctg ttaaactaag 7681 acttattatc taaacgtaat taaatgttga tatcttataa tgctccactt tgaaacagct 7741 gtctttcttt tgcagattag cgatgatgaa ccaggttatg acctagattt gttttgtata 7801 cctaatcatt atgccgagga tttggaaaaa gtgtttattc ctcatggact gattatggac 7861 aggttagtaa gacctcgatt gaagttattt ttagagggtg tgtgtgtggt gtgtgtgtgt 7921 gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa gagagagagg agaggcatgt gtgatatata 7981 tgtgaagtgt attgcatgta catggaatcc agagaaggac attaggtgtc ctactctgtt 8041 gctctcttat tcccttgaat cagtgtctct tgctgaacct ggggcataca tactgatttg 8101 gctaggctgc ctggctagca agacccaggg atctgcttgt ctctgccccc cagtgctgac 8161 gttacaagca ggtgcaacca catctggttt ttatatgggt actgggggat ctgaacttag 8221 ggcctgatgc gtgcccaagt gctctcaccc gccaagccac ctctccagcc aaagggtttt 8281 ttttcttgta ttaattctaa cttagcaact gttgagtagt tgatgtttga atataagcta 8341 agccaactta ttagaataaa tgttttctat tttttacttg tatgtgtgag tgcctgcatg 8401 tatgtatgtg aagcatatgt attccaggat gtcagaattg tgatggattc cctggaactg 8461 gagttacaga ccattgtgaa ccactgtgta tgggtgctgg gaactgaact ttaggtcctc 8521 tgcaaaaaca gtaaggatct taactgataa actatctttc tagccctaag gctttctact 8581 tttaaagttc gtttctcttt ctctctctct ctctctctct ctctctctct ctccctctcc 8641 ctccctcctc ctcctcctcc tctttccccc ctctcgcctc ctctttctct ctcccctccc 8701 tctctctctc tcacacacac acacagacag agagaggaga gagaaaggaa gccttactaa 8761 ttaattacct agttttagac ataactagtg ggaatccata gtaagtaaat caacattctt 8821 gaagagaggt tagaagaatt ggctctcgtt gtccatcgag gctgtataag attattcttc 8881 tgttttctca gcttaccata catattttct acgtagacaa taccaattca aatacactgt 8941 acgtaaagta tatgcgcaca cacacacaca cacacacaca cacacacaca caagttacat 9001 attgtcatat agtactaaaa tttatggact ttttcatatt aatacatcta gagctatcat 9061 gttttttatc ttttaaaaat tactttacgg ggctacagag atgtctcagc agttaggagc 9121 actgactgct cttccagagg tcctgagttc aattcccagc aaccacatgg tggctcacaa 9181 ccatctgtaa tgggatctga tccactctta tggtgtgtct gaagacagtg acagtgtact 9241 catatacatg aaataaatct ttaaaaaatt actttaccat cagggtatat atatatatat 9301 atatgtgtgt gtgtgtgtgt gtgtgtatga agtatttgct atttgagagg gtctcactgt 9361 ttagctcagt ctggcttgga acgttctgta tagcccagac tggcctcaac tcagcagtcc 9421 ccctgaaagg ctctcatatg tggagattac aggtatgtgc tactgtgccc agccctacat 9481 gttactttgt ttttgaaata gggtatcaaa tagcttagac tggcttctaa gtcagtgtgt 9541 agctaaggat ggtctgagct tctgaacctg ctacttcaac tcctggtgtg ccatcatggc 9601 cagtttgcac agttgtgaga gtagaactca ggacctccta tgtgttacta gacatgttct 9661 ctaccagctg agctacatcc caattcgcct acatttactt ttatttttta aaagatttat 9721 ttatgtatac agcactctgc ctgcatgtac atctgcatgc cagaagaggg caccagatct 9781 catcatacat agttgtgagc caccatgtgg ttgctgggga ttgaactcag ggcctctgga 9841 agagcagtca gtgctcttaa cctctgagcc atctctgtag ccccctacat ttattttaaa 9901 gattataggc tattctattc atagatgtgc cataactgat ttaaccagat cttcattatt 9961 ttgtacttag tagagtatat tagagggaat gaatcttaaa tacacatttt gacacttgct 10021 cagctttaga caagtaaaaa tcactggtca aggaaatggt gcttagtgga tgcttctgaa 10081 ctgtcaaatt gtcctccaac aagttacatc attttatact tccaacgtta tgtgagagtt 10141 catttttctt taggctcttc tagtatttta tatttgagaa ataaaattta accaaaattt 10201 gccaatctag taagcttgtt tttatttgta tctttaaatt ttatgataga tgttatagtg 10261 tactctcctc tcccctcccc tcccctctcc tcctcagcag gatcttgtta tgtagcccta 10321 agggatctaa tacttactct gtagtccagg ctgtccttca gctcatgaga cacaccatca 10381 tgcttggcat gtttcttgtt ctgaaaggta tctggtttta attttggttg agctggtgtg 10441 ttggtgtata tctgtaatct cagcacaggc aagtagagac aaggattatg attacagtcc 10501 agcctgggct atttagaggg attctcttta atctttcctc atgccccaaa atcttacctt 10561 tggtatatga aaaatagtct ccacttctgc aaaatattgc tttatgaagt aagaattaat 10621 tcccttcata gagacaagga atgtgtcctg taaaagttta atgtgtaaga agtatttgtt 10681 ataaaagata aatattcaga atcttctttt taattcctga ttttatttct ataggactga 10741 aagacttgct cgagatgtca tgaaggagat gggaggccat cacattgtgg ccctctgtgt 10801 gctcaagggg ggctataagt tctttgctga cctgctggat tacattaaag cactgaatag 10861 aaatagtgat agatccattc ctatgactgt agattttatc agactgaaga gctactgtgt 10921 aagtataatt aacttataat taaaaaaata gggccattct agttttatct atattttttt 10981 taaacttgtg caaactatgc tacataatca acttacttga ttatgtaata tatatagtaa 11041 tagttggaat tactattatt acatatttat ttttctgtgc tggggtagaa cccagggaac 11101 taagtactgt aataaccgag cttcattcat cctagtcatt tttaattttt tttttttttt 11161 ttgttttttc gagacaaggt ttctctgtgt agccctggct gtcctggaac tcactctgta 11221 gaccaggctg gcctcgaact cagaaatctg cctgcctctg cctcccagtg ctgagattaa 11281 tgacgtgcac caccactgcc tggcattttt ttaaagtttt aatttgagac cctttctcac 11341 taacttgctc acactatcct cgaactcatt acgtgggctg gtctagaaat ttcagtcaac 11401 ttgcctcagc ttctcaagta gctgggatta caggcaggtg ctaccacttc tggccatgat 11461 cattgttgtt gttgctatta ttattattat tatttcactg atacttcata tcacaacaca 11521 gaatagtaac tgtagttcat gaccaacaaa aaagttgtac agtttctctg ttgtcttatt 11581 ttgatctgga ttttgatttg tttcatggca ctgggcaggc taatctcaaa ctgcatggct 11641 caaatagtcc ttctgtctta atctcccatg ttctggaagt ataggtatca ctacacctag 11701 ctgtattttc atattttaaa aaaaacgcct aagaattgct attgaatgag gcggagagat 11761 ggctcagcag tcacaagtac ttgtagagga ctggggtttg gttactagca cctcaaagat 11821 ggcttacaat tatctgtagc tccaattcca gaggatctgt gtggtaaagc tgcatgtata 11881 tgggcatgca ggtaaaactg ctaaacatat aaaaatagct cagtcttaag agaattggtc 11941 gactttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 12001 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 12061 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 12121 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 12181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 12241 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 12301 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 12361 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 12421 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 12481 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 12541 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 12601 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 12661 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 12721 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 12781 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 12841 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 12901 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 12961 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 13021 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 13081 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 13141 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 13201 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 13261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 13321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaggtcgag tagggataac 13381 agggtaatgg atcctcgttg ggcacctgca ctcatgtacg caggcccaca tatagatgta 13441 cacacgcata tcattcaaaa tagaaattat ttatacaagt agtgcgttgg agagctggct 13501 caggagttgt taatgcttgc tacccttgca aaggacccaa gttcaatttc cagcacctgt 13561 gttgggcatc tcacaaccac atgtaaatct agtttcaggg gatttcaccc ctcttctgaa 13621 ctcttcaggc acttataccc acaaatggac atctccacat agagacacat acttttaaaa 13681 aagtaatcta gggtctagag aaatggttat agaccacgga ctgggatcag ttcccagaag 13741 ccacatcctg tggctcacaa ttgcctgtga atcaagttct agatctggtg gcctcgtcta 13801 gcctgcagtc atcaacaaac acatgcacac atccatacac acataattta aaagaaagcg 13861 aaagtaacct ataatgctaa acaaagcaca ttctcagtgt agactgcagt ggtgatgaac 13921 cttgaaaata tattaataga agccagacac gtaagtcaca aatggtataa ctcaatttat 13981 atgaaattcc cagagagtcc aaagttaaaa aaaacaaaaa ggcagattat tggttgtcag 14041 tagctacaga gatgggacat tgaagatatt aataggtata gggttgagga ttcatggcct 14101 ttccctcttc catgttagca tgtctattgt tgttaacctt attcaactca tgttagggca 14161 atcatattga tgagacttaa tgagtatagc ttgacattcc tagaagacag agtctcacat 14221 cagactctct gatcctctga ctcttagaat ctttcaacac cctcttctga aatgatctct 14281 gagcctttgg ttgtgactga attatgagaa agaattttat gttgaatgaa ttttacttca 14341 gcatttttac aaaactgaag ctggggatgg cgtttcatgc gtgcagtccc taccatttgc 14401 ctataagcct ggaggaggca gaagtagaat taggcttttc agggacatct tcagctgcat 14461 catgagcttg aggacaactt gagctacata agattcccat ccccaccccc acacacacac 14521 acacatctag tatgtaccta gtatgcagtc ttttcatatg tttaaaatgt agaaaggtat 14581 atgtgaagtc tcccttttcc ccacaactta gtccctccaa agtattgcat cagtttcctt 14641 gtacatgatt ttatgcatat ataagataca ctatttctct ctctctctct ctccttccct 14701 ccttccctcc aacctctggg tatgagttga gggttatgta tgtttcaagt tgtaataaga 14761 attgccactc aaacatttca tcccatttta agttaagttt gtctgatttt ccactttaga 14821 agggcagcca tgatggctca tgcctataat cccatcctag cacttgtaag cctaatacat 14881 aagactggag gattgtcatc agcttgaggc tagcctggtc tacctaatga gttccagcca 14941 gccaagagta gaatataaga ccctgtctcc aaaaaatgta ccaaaggggc tggaggggta 15001 actcagtggt taagaacagt agctgctctt ccagaggacc tgggttcaat tcccagcacc 15061 cacatggcag cttgcaacta tctgtaactg tccagctttg ggatatccga caccattata 15121 ccaattacat aaaatgaaaa aattgtaaaa tgtgctaaaa tgagtgggga gggggagcat 15181 ggtgtggttc tgtgggcttg ggattttgcc atgaatccat gactatatct cagcaatatg 15241 tggattattt ctggaaacct gtggggcatg ggtgtatggg aagaggggag agagagagag 15301 cccgcatcca gccagagttc ctgtgctctg ggcaggcgga cgtgggagga ctgctggacg 15361 atttccactt ggccctgggt gggcatctgg ctgtgtgaag ccactgaccc cactcagcgg 15421 ggggtagata aggggcattt cccataccag gttctcagac tccagcattc cacgctggat 15481 acagcagaag agctgagaac agaatagggg ccccagggag acactctcgt cccagagata 15541 aagaaggcag agggagagag attcctacgc aggcgagagg ccttagtctg ggccttgatg 15601 aacagagacg gtctatggtt ttagagcttt attgtagaaa ggcaggggga aagagagaag 15661 gtagaaagag agagaccagc catggccaag aggagagaag gggggaaagg agagagagaa 15721 gaaaggctag agagtaagaa aggttaaaga gacagaagta aaagcttaga gtaagagaga 15781 gagagagagc aagagggaga gtaagaaaag taaaagctta agagagcgag gaggggccag 15841 cccctctaat agtgggcttg ttatcttgtt gttgctaggt aactgtgaag cgtctagcct 15901 gaaggccaga agcttgggac attgtctatg tgactgaaag ccatgagcct ctactgtggg 15961 ggctgtggag gtggtaactt ggacaggagc caggggttcc aggagacatg agggaatgcc 16021 ttctgtccca tgtaggtagg aattaccacc accggctttc accgtagttc actggagact 16081 gtttgtacat agcccattgc cccacagaaa ccaggcactc actgtttcat ttgcataaga 16141 attagaaggc tttattttgg ctgggattgg tgacttgccc cagaaattcc agcttttggg 16201 aagccgaatc ctggagaacc aggagttcaa ggtcatcctc aggtacctca tttgttcaag 16261 accagcctgt gctaccatga gaccttgtcc ctactcccct caaccccaaa acacgctgtt 16321 ttcattttat aggttgtact aaccatggag gatggtcatt tgcctttctc ttgatttcag 16381 aagcgtagcg cgggctttgc accatgatct agtgtttcca gataggcttg cctctccaca 16441 gccaggctta tcctgcttca gtcccatgtt tggcaagggc aacatatgac tgaagaactt 16501 gcatagcctt atggtgtatc atgactctta gccccagttt ataactgtct aacagctggt 16561 ggcttttgtg aaaaaggaaa gcatcatttg aacttttaac ttttcagctg tttgttttga 16621 ctttgcataa atactgagac tagctaggaa atgagctttt gtttcatttg ctaaaaatga 16681 tttttttttc atgaggcaat ttcaagcaat ttgaatttag caatctctac ttgtctgaaa 16741 agaagttatt aattaaaaaa aaacaaacaa acaggaacct attatgctgg ctagtcacat 16801 gaaagaatcc aaatgaacac agtcaaggct gaatcaacac atgaaattat tttctaattc 16861 cccaaattac atttatgcta attagtatgt agtaaaacat tttaattgtt tccaatgtta 16921 aacattacag aaataatttt gatgttttcc taataaccca gcctttgatc atgtttgcat 16981 ttatttgatt gttgtcagtt tattaaataa ttaagtaaga gcaacaagat aaaatgactt 17041 catgagagtc cttttctgta acttagaacg tcagtagtca taggaactgc ggtcgttccc 17101 ttacctttca gaagtaggct aaagagttga acgcaaaggt ggtttttcga atcttcctga 17161 tccttaagcc cccactaggt ttatattcta tatttgatat cggattcgaa cccctcgagc 17221 ttaggatccg gaacccttaa tataactaag ttattaggtc cctcgaagag gttcactagt 17281 actggccatt gcggccgctt cgaggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 17341 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 17401 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 17461 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 17521 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 17581 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 17641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 17701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 17761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 17821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 17881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 17941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 18001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 18061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 18121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 18181 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 18241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 18301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 18361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 18421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 18481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 18541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 18601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 18661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 18721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 18781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 18841 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 18901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 18961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 19021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 19081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 19141 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 19201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 19261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 19321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 19381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 19441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 19501 atttccccga aaagtgccac ctgacgg //