LOCUS       pSicoR-Ef1a-mCh-GFPi        7525 bp    DNA     circular     23-JUN-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|593687039|
COMMENT     VNTDBDATE|593687623|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSicoR-Ef1a-mCh-GFPi|
COMMENT     VNTAUTHORNAME|Matthew Spindler|
COMMENT     VNTAUTHORTEL|415-734-2833|
COMMENT     VNTAUTHORFAX|415-355-0960|
COMMENT     VNTAUTHOREML|mspindler@gladstone.ucsf.edu|
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FEATURES             Location/Qualifiers
     misc_feature    1076..1213
                     /vntifkey="21"
                     /label=Psi
     misc_feature    1707..1910
                     /vntifkey="21"
                     /label=RRE
     promoter        2631..2944
                     /vntifkey="29"
                     /label=U6\Promoter
     misc_feature    2826..2859
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4325..4358
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4414..4793
                     /vntifkey="21"
                     /label=WRE
     primer          2481..2500
                     /vntifkey="27"
                     /label=Lox\Replace\For\Primer
     primer          complement(493..512)
                     /vntifkey="27"
                     /label=Lox\Replace\Rev\Primer
     misc_marker     3595..4305
                     /vntifkey="22"
                     /label=mCherry
     promoter        3030..3572
                     /vntifkey="29"
                     /label=EF-1alpha\promoter
     misc_feature    complement(6535..7392)
                     /vntifkey="21"
                     /label=AmpR
     insertion_seq   2945..2999
                     /vntifkey="14"
                     /label=GFPi
BASE COUNT     1933 a      1856 c      1937 g      1799 t 
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 
     2641 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact 
     2701 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg 
     2761 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga 
     2821 tttctataac ttcgtatagc atacattata cgaagttata catgtcacaa aaggaaactc 
     2881 accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg agaaaagcct 
     2941 tgtttacagc cacaacgtct atatttcaag agaatataga cgttgtggct gtttttttct 
     3001 cgagtactag gatccattag gcggccgcgg atctgcgatc gctccggtgc ccgtcagtgg 
     3061 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc 
     3121 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 
     3181 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 
     3241 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 
     3301 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 
     3361 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 
     3421 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 
     3481 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 
     3541 cgttacagat ccaagctgtg accggcgcct acgctagcgc taccggtcgc caccatggtg 
     3601 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 
     3661 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 
     3721 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 
     3781 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 
     3841 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 
     3901 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 
     3961 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 
     4021 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 
     4081 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 
     4141 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 
     4201 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 
     4261 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagaatt cgtcgaggga 
     4321 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc 
     4381 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt 
     4441 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     4501 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     4561 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     4621 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     4681 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     4741 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     4801 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 
     4861 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     4921 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     4981 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca 
     5041 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc 
     5101 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac 
     5161 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 
     5221 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta 
     5281 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg 
     5341 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga 
     5401 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt 
     5461 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc 
     5521 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 
     5581 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 
     5641 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 
     5701 aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     5761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     5821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     5881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     5941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     6001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     6061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     6121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     6181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 
     6241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     6301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     6361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     6421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     6481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     6541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     6601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     6661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     6721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     6781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     6841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     6901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     6961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     7021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     7081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     7141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     7201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     7261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     7321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     7381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     7441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     7501 atttccccga aaagtgccac ctgac 
//
