LOCUS 48672\M-ST1-sgRN 3147 bp DNA circular 26-SEP-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|674126472| COMMENT VNTDBDATE|674126472| COMMENT LSOWNER| COMMENT VNTNAME|48672 M-ST1-sgRNA| COMMENT VNTAUTHORNAME|Kevin Esvelt| COMMENT VNTAUTHOREML|kesvelt@gmail.com| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.44525 1.9 COMMENT 0.082322 1) COMMENT #174=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #81# 23690260 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 0 COMMENT "{\\i Bam}HI (918)" "@N (@S)" 5 0 0 -8.19537 COMMENT 10.8425 3.35897 0.615385 #173#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #175=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList) (CObjectList)) COMMENT #176=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -394 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 2082602632 2082602632 (CObList))) FEATURES Location/Qualifiers rep_origin 1317..1984 /vntifkey="33" /label=pUC\origin CDS complement(2132..2947) /vntifkey="4" /label=KanR promoter 432..749 /vntifkey="29" /label=U6\promoter misc_feature 750..769 /vntifkey="21" /label=spacer misc_feature 432..913 /vntifkey="21" /label=U6\promoter\-\ST1m1\sgRNA\(orientation\unknown) misc_feature 770..879 /vntifkey="21" /label=ST1m1\sgRNA BASE COUNT 855 a 751 c 744 g 797 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 421 tgcatctaga ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg 481 gtaccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac 541 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag 601 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 661 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 721 tatatatctt gtggaaagga cgaaacaccg tcccctccac cccacagtgg tcccctccac 781 cccacagtgg tttttgtact ctcagaaatg cagaagctac aaagataagg cttcatgccg 841 aaatcaacac cctgtcattt tatggcaggg tgtttttttc tagacccagc tttcttgtac 901 aaagttggca ttaatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg 961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 1021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 1081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 1141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 1201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 1261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 1321 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 1381 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 1441 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 1501 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 1561 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 1621 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 1681 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 1741 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 1801 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1861 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 1921 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 1981 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 2041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 2101 aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa 2161 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta 2221 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg 2281 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt 2341 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat 2401 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg 2461 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc 2521 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg 2581 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc 2641 cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg 2701 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat 2761 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca 2821 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata 2881 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat 2941 ggctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 3001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 3061 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 3121 ataggcgtat cacgaggccc tttcgtc //