LOCUS pHJ18 5820 bp DNA circular 26-JUL-2013 FEATURES Location/Qualifiers gene 1..905 /note="bTUB gene array" 5'UTR 913..1103 /note="5'UTR ALD" repeat_unit 1109..1142 /note="loxP" misc_difference 1152..1305 /note="Procyclin SAS" CDS 1311..1907 /note="PUR" misc_feature 1914..1943 /note="Ty1" CDS 1950..3080 /note="HSVTK" repeat_unit 3087..3120 /note="loxP" 3'UTR 3122..3278 /note="3'UTR ALD " CDS 3965..4822 /note="AMP" primer_bind complement(3124..3144) /note="PCR-KO reverse" primer_bind 1080..1100 /note="PCR-KO forward" primer_bind complement(1908..1931) /note="oligo #44" source 1..5820 /dnas_title="pHJ18 (pHJ10 with single NheI)" ORIGIN 1 CTGACCGTAT CATGATGACT TTCTCCATCA TCCCATCCCC CAAGGTGTCC GACACTGTCG 61 TCGAGCCGTA CAATACGACT CTCTCCGTGC ACCAACTTGT GGAAAACTCC GATGAGTCGA 121 TGTGCATTGA CAACGAGGCA CTGTACGATA TTTGCTTCCG CACCCTGAAA CTGACAACAC 181 CAACGTTCGG TGACCTGAAC CACTTGGTGT CTGCTGTTGT GTCCGGCGTC ACCTGCTGCC 241 TGCGCTTCCC TGGTCAGTTG AACTCTGACC TCCGTAAGTT GGCTGTGAAC CTTGTCCCAT 301 TCCCGCGTCT GCACTTCTTC ATGATGGGCT TCGCCCCGCT GACCAGCCGC GGCTCGCAGC 361 AGTACCGCGG TCTCTCCGTG CCCGAGCTAA CGCAGCAGAT GTTCGATGCG AAAAACATGA 421 TGCAAGCTGC AGATCCTCGT CACGGCCGCT ACCTGACAGC GTCTGCACTC TTCCGCGGCC 481 GCATGTCGAC GAAGGAGGTT GATGAGCAGA TGCTGAACGT GCAGAACAAG AACTCGTCCT 541 ACTTCATTGA GTGGATCGAT CCCGAACAAC ATCAAGTCCT CTGTTTGCGA TATCCCACCC 601 AAGGGACTCA AGATGGCTGT CACCTTCATT GGCAACAACA CCTGCATCCA GGAGATGTTC 661 CGCCGTGTGG GAGAGCAGTT CACCCTCATG TTCCGTCGCA AGGCGTTCTT GCACTGGTAC 721 ACTGGCGAGG GTATGGACGA GATGGAATTC ACGGAGGCAG AGTCCAACAT GAACGATCTC 781 GTGTCTGAGT ACCAGCAGTA CCAGGATGCC ACGATTGAGG AGGAGGGCGA GTTCGACGAG 841 GAGGAGCAAT ACTAGACGCG GACGGGGCAT TTCCCGTTCG TCATTAGCAG TAGGTAATGA 901 AGATGCTCGA GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA 961 GGCACGGAAG CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA 1021 ATATTTGCGA TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA 1081 AAACATAAAC TCAACTGCAA CGAAGCTTAT AACTTCGTAT AGCATACATT ATACGAAGTT 1141 ATaccggtAC AcGTTCTCGT Cggccggccg ctgcacgcgc cttcgagttt tttttccttt 1201 tccccatttt tttcaacttg aAGaCTTCAA TTACACCAAA AAATAAAATT CACAAACTTG 1261 GtATTCCTTT GTGTTACATT CTTGATCGCT CGCACTGACA TTACagatct ATGACCGAGT 1321 ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG 1381 CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCAGAC CGCCACATCG 1441 AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG 1501 TGTGGGTCGC GGACGACGGC GCAGCAGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG 1561 CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG 1621 CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT 1681 TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG 1741 TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG 1801 CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG 1861 TGCCCGAAGG ACCGCGCACT TGGTGCATGA CCCGCAAGCC CGGTGCCtCT AGAGAAGTCC 1921 ATACTAACCA GGACCCACTT GACGCCACCA TGGCCTCGTA CCCCGGCCAT CAACACGCGT 1981 CTGCGTTCGA CCAGGCTGCG CGTTCTCGCG GCCATAGCAA CCGACGTACG GCGTTGCGCC 2041 CTCGCCGGCA GCAAGAAGCC ACGGAAGTCC GCCCGGAGCA GAAAATGCCC ACGCTACTGC 2101 GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA CTGCTGGTGG 2161 CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC TGGCGGGTGC 2221 TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC GACCAGGGTG 2281 AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA ATGGGCATGC 2341 CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT GGGAGCTCAC 2401 ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC GCCCTCCTGT 2461 GCTACCCGGC CGCGCGGTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG CTGGCGTTCG 2521 TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CCAACATCGT GCTTGGGGCC CTTCCGGAGG 2581 ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTG GACCTGGCTA 2641 TGCTGGCTGC GATTCGCCGC GTTTACGGGC TACTTGCCAA TACGGTGCGG TATCTGCAGT 2701 GCGGCGGGTC GTGGCGGGAG GACTGGGGAC AGCTTTCGGG GACGGCCGTG CCGCCCCAGG 2761 GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG TTATTTACCC 2821 TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG TTTGCCTGGG 2881 CCTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG GATTACGACC 2941 AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG GTCCAGACCC 3001 ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG TTTGCCCGGG 3061 AGATGGGGGA GGCTAACTGA GAATTCATAA CTTCGTATAG CATACATTAT ACGAAGTTAT 3121 GGATCCTGCC CATTTAGTTA GTTGGCTTTT CCCTTGTCTC GTGTCTTTTC CGTGGAAAGG 3181 TTCCCGGAGT AATCTGATGG CACAGCAGGG AGGTGCGCCT GCAGGTTGGT TAGGAAGGGG 3241 GGATGATGTA AAAGAAGAAA ATGGGGGGAT TCGAGCCCCC TTGCAGGCAT GCAAGCTAGC 3301 CAGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT 3361 GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG 3421 AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC 3481 ATCTCCTTGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT 3541 GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGATA TGGTGCACTC TCAGTACAAT 3601 CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC 3661 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG 3721 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT 3781 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 3841 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 3901 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 3961 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 4021 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 4081 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 4141 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 4201 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 4261 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 4321 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 4381 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 4441 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 4501 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 4561 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 4621 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 4681 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 4741 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 4801 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 4861 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 4921 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 4981 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 5041 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 5101 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 5161 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 5221 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 5281 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 5341 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 5401 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 5461 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 5521 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 5581 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 5641 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 5701 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 5761 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG //