LOCUS 48669\M-ST1cas 8822 bp DNA circular SYN 25-SEP-2013 DEFINITION pcDNA3.3 TOPO TA. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|674085010| COMMENT VNTDBDATE|674085010| COMMENT LSOWNER| COMMENT VNTNAME|48669 M-ST1cas| COMMENT VNTAUTHORNAME|Kevin Esvelt| COMMENT VNTAUTHOREML|kesvelt@gmail.com| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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terminator" "@N" 1 0 0 9.71692 COMMENT 12.3243 6.5641 0.820513 #276#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #278=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 18 100) COMMENT (CObjectList COMMENT #279=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 16646280 100) COMMENT (CObjectList COMMENT #280=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #185# 7761016 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 3.68342 1.9 COMMENT 0.082322 1) COMMENT #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #187# 18 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Asc}I (3643)" "@N (@S)" 5 0 0 -4.67768 COMMENT 5.99088 2.89744 0.615385 #280#)) COMMENT (CObjectList)) COMMENT #282=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 1002 100) COMMENT (CObjectList COMMENT #283=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #185# 9 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.25157 1.9 COMMENT 0.082322 1) COMMENT #284=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #187# 880 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Sbf}I (2844)" "@N (@S)" 5 0 0 -7.11616 COMMENT 4.7242 2.87179 0.615385 #283#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #285=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 16646296 100) COMMENT (CObjectList) (CObjectList)) COMMENT #286=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1344 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2082602632 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_feature 130..417 /vntifkey="21" /label=CAG_enhancer promoter 51..627 /vntifkey="30" /label=CMV_immearly_promoter misc_feature 584..604 /vntifkey="21" /label=CMV_fwd_primer promoter 585..654 /vntifkey="30" /label=CMV_promoter misc_feature 628..647 /vntifkey="21" /label=pCEP_fwd_primer misc_feature 630..654 /vntifkey="21" /label=LNCX_primer promoter 597..716 /vntifkey="30" /label=CMV2_promoter terminator 4195..4465 /vntifkey="43" /label=TK_PA_terminator rep_origin 4519..4825 /vntifkey="33" /label=f1_origin misc_feature complement(4939..4959) /vntifkey="21" /label=pBABE_3_primer misc_feature complement(4945..5160) /vntifkey="21" /label=SV40_enhancer promoter 4957..5225 /vntifkey="30" /label=SV40_promoter rep_origin 5124..5201 /vntifkey="33" /label=SV40_origin misc_feature 5186..5205 /vntifkey="21" /label=SV40pro_F_primer CDS 5340..6134 /gene="NeoR/KanR" /vntifkey="4" /label=NeoR/KanR /note="ORF frame 2" gene 5343..6131 /gene="NeoR/KanR" /vntifkey="60" terminator 6313..6432 /vntifkey="43" /label=SV40_PA_terminator misc_feature 6401..6420 /vntifkey="21" /label=EBV_rev_primer misc_feature complement(6476..6494) /vntifkey="21" /label=M13_reverse_primer misc_feature complement(6493..6515) /vntifkey="21" /label=M13_pUC_rev_primer promoter complement(6529..6558) /vntifkey="30" /label=lac_promoter rep_origin complement(6867..7486) /vntifkey="33" /label=pBR322_origin CDS complement(7641..8501) /gene="Ampicillin" /vntifkey="4" /label=Ampicillin /note="ORF frame 2" gene complement(7641..8501) /gene="Ampicillin" /vntifkey="60" promoter complement(8543..8571) /vntifkey="30" /label=AmpR_promoter misc_feature 4111..4143 /vntifkey="21" /label=NLS CDS 748..4110 /vntifkey="4" /label=ST1cas BASE COUNT 2172 a 2512 c 2365 g 1773 t ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tgccaccatg agcgacctgg tgctgggcct ggacatcggc 781 atcggcagcg tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac 841 agtcgcatct tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag 901 ggtcgccggc ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag 961 gagagcggcc tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg 1021 cgcgtgaagg gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac 1081 atggtgaagc accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc 1141 gtgggcgact acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc 1201 ggccagatcc agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg 1261 gagaaggacg gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc 1321 gaggccctgc gcatcctgca gacccagcag gagttcaacc cccagatcac cgacgagttc 1381 atcaaccgct acctggagat cctgaccggc aagcgcaagt actaccacgg ccccggcaac 1441 gagaagagcc gcaccgacta cggccgctac cgcaccagcg gcgagaccct ggacaacatc 1501 ttcggcatcc tgatcggcaa gtgcaccttc taccccgacg agttccgcgc cgccaaggcc 1561 agctacaccg cccaggagtt caacctgctg aacgacctga acaacctgac cgtgcccacc 1621 gagaccaaga agctgagcaa ggagcagaag aaccagatca tcaactacgt gaagaacgag 1681 aaggccatgg gccccgccaa gctgttcaag tacatcgcca agctgctgag ctgcgacgtg 1741 gccgacatca agggctaccg catcgacaag agcggcaagg ccgagatcca caccttcgag 1801 gcctaccgca agatgaagac cctggagacc ctggacatcg agcagatgga ccgcgagacc 1861 ctggacaagc tggcctacgt gctgaccctg aacaccgagc gcgagggcat ccaggaggcc 1921 ctggagcacg agttcgccga cggcagcttc agccagaagc aggtggacga gctggtgcag 1981 ttccgcaagg ccaacagcag catcttcggc aagggctggc acaacttcag cgtgaagctg 2041 atgatggagc tgatccccga gctgtacgag accagcgagg agcagatgac catcctgacc 2101 cgcctgggca agcagaagac caccagcagc agcaacaaga ccaagtacat cgacgagaag 2161 ctgctgaccg aggagatcta caaccccgtg gtggccaaga gcgtgcgcca ggccatcaag 2221 atcgtgaacg ccgccatcaa ggagtacggc gacttcgaca acatcgtgat cgagatggcc 2281 cgcgagacca acgaggacga cgagaagaag gccatccaga agatccagaa ggccaacaag 2341 gacgagaagg acgccgccat gctgaaggcc gccaaccagt acaacggcaa ggccgagctg 2401 ccccacagcg tgttccacgg ccacaagcag ctggccacca agatccgcct gtggcaccag 2461 cagggcgagc gctgcctgta caccggcaag accatcagca tccacgacct gatcaacaac 2521 agcaaccagt tcgaggtgga ccacatcctg cccctgagca tcaccttcga cgacagcctg 2581 gccaacaagg tgctggtgta cgccaccgcc aaccaggaga agggccagcg caccccctac 2641 caggccctgg acagcatgga cgacgcctgg agcttccgcg agctgaaggc cttcgtgcgc 2701 gagagcaaga ccctgagcaa caagaagaag gagtacctgc tgaccgagga ggacatcagc 2761 aagttcgacg tgcgcaagaa gttcatcgag cgcaacctgg tggacacccg ctacgccagc 2821 cgcgtggtgc tgaacgccct gcaggagcac ttccgcgccc acaagatcga caccaaggtg 2881 agcgtggtgc gcggccagtt caccagccag ctgcgccgcc actggggcat cgagaagacc 2941 cgcgacacct accaccacca cgccgtggac gccctgatca ttgcggcttc tagccagctg 3001 aacctgtgga agaagcagaa gaacaccctg gtgagctaca gcgaggacca gctgctggac 3061 atcgagaccg gcgagctgat cagcgacgac gagtacaagg agagcgtgtt caaggccccc 3121 taccagcact tcgtggacac cctgaagagc aaggagttcg aggacagcat cctgttcagc 3181 taccaggtgg acagcaagtt caaccgcaag atcagcgacg ccaccatcta cgccacccgc 3241 caggccaagg tgggcaagga caaggccgac gagacctacg tgctgggcaa gatcaaggac 3301 atctacaccc aggacggcta cgacgccttc atgaagatct acaagaagga caagagcaag 3361 ttcctgatgt accgccacga cccccagacc ttcgagaagg tgatcgagcc catcctggag 3421 aactacccca acaagcagat caacgataaa ggcaaggagg tgccctgcaa ccccttcctg 3481 aagtacaagg aggagcacgg ctacatccgc aagtacagca agaagggcaa cggccccgag 3541 atcaagagcc tgaagtacta cgacagcaag ctgggcaacc acatcgacat cacccccaag 3601 gacagcaaca acaaggtggt gctgcagagc gtgagcccct ggcgcgccga cgtgtacttc 3661 aacaagacca ccggcaagta cgagatcctg ggcctgaagt acgccgacct gcagtttgat 3721 aagggcaccg gcacctacaa gatcagccag gagaagtaca acgacatcaa gaagaaggag 3781 ggcgtggaca gcgacagcga gttcaagttc accctgtaca agaacgacct tctgctggtg 3841 aaggacaccg agaccaagga gcaacagctg ttccgcttcc tgagccgcac catgcccaag 3901 cagaagcact acgtggagct gaagccctac gacaagcaga agttcgaggg cggcgaggcc 3961 ctgatcaagg tgctgggcaa cgtggccaac agcggccagt gcaagaaggg cctgggcaag 4021 agcaacatca gcatctacaa ggtgcgcacc gacgtgctgg gcaaccagca catcatcaag 4081 aacgagggcg acaagcccaa gctggacttc agcagggctg accccaagaa gaagaggaag 4141 gtgtgactct agatgaaagg gttcgatccc taccggttag taatgagttt aaacggggga 4201 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 4261 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 4321 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 4381 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 4441 gtcggggcgg cagaccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc 4501 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 4561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 4621 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt 4681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 4741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 4801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 4861 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 4921 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 4981 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 5041 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 5101 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 5161 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 5221 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 5281 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 5341 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 5401 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 5461 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 5521 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 5581 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 5641 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 5701 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 5761 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 5821 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 5881 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 5941 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 6001 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 6061 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 6121 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac 6181 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 6241 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 6301 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6361 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6421 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 6481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 6541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 6601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 6661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 6721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 6781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 6841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 6961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 7021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 7081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 7141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 7201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 7261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 7321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 7381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 7441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 7501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 7561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 7621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 8581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 8641 ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg 8701 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga 8761 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta 8821 gg //