LOCUS       Ple53\(pEMS1199)       19968 bp    DNA     circular     17-JUN-2009
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C
            Antisense primer: oEMS2753 - 5' - GCTGCCACCAAGTGGGATAAA-3'  Tm=56.3 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            735 bp product
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|521216110|
COMMENT     VNTDBDATE|521291202|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple53 (pEMS1199)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
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COMMENT              (CObjectList 
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COMMENT               #144=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -116 100) 
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COMMENT                             #148=(CScratch 
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COMMENT                                   "MFRT (F5mutant)" "@N" 1 14.9041 6.96932 0 
COMMENT                                   -10 7.91752 1.17389 #148#) 
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COMMENT                                   "Tetracysteine residue" "@N" 1 16.2902 
COMMENT                                   1.74233 0 -10 10.0405 1.17389 #154#) 
COMMENT                             #156=(CScratch 
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COMMENT                                   12.873 0 0 -10 1.99811 1.17389 #156#) 
COMMENT                             #158=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.84658 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   13.1215 -1.74233 0 -10 2.72243 1.17389 #158#) 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 16.8915 
COMMENT                                   -3.48466 0 -10 11.0645 1.17389 #160#) 
COMMENT                             #162=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.03222 1.9 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                             #168=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -4.38824 12.1963 0 -10 2.87229 1.17389 #167#) 
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COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #108# 798 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.25653 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2364" "@N" 
COMMENT                                   1 3.23685 12.1963 0 -10 4.79547 1.17389 #172#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #174=(CGroupWidget 
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COMMENT                                   3.48466 0 -10 10.4901 1.17389 #175#) 
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COMMENT                                   "3' homology sequence" "@N" 1 -7.56613 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #177#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 16.9781 
COMMENT                                   -6.96932 0 -10 14.9609 1.17389 #179#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #181=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -322 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #182=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -391 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #183=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1019 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
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COMMENT         #185=(AnlzFontBackColorItem 138 766 40304639) 
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FEATURES             Location/Qualifiers
     misc_recomb     13831..17642
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(18741..19962)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     12380..13805
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     8739..12384
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     4785..8738
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    13812..13829
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    3667..3700
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    3701..3711
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    3708..4427
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4428..4463
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    4464..4536
                     /vntifkey="21"
                     /label=NLS
     misc_feature    4542..4575
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    4729..4779
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..3658
                     /vntifkey="21"
                     /label=Ple53\(DCX-B)
                     /note="pEMS1306 - DCX-B MiniP is made up of the region "LongProm""
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     misc_feature    768..788
                     /vntifkey="21"
                     /label=oEMS2753
                     /note="Note: there is a A>G mismatch at bp 7 (to 5' end) but still amplifies"
BASE COUNT     5371 a      4408 c      4389 g      5800 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccgg aacacctatt aatgcccaca tatctcaaac aaggaaatat 
      181 tgatcctgcc tatccctctg ctctctatta tatataaagc aaacaaagtc atttatccta 
      241 ggctgtctca taaaaacatt ttgtagcttc cacagtagca ttgcaaagcc tatattatga 
      301 ggaattttcc tctgaaatac attccaaatg gttcgtgttt aagttttatt atcccatcca 
      361 ttgttgtatc ttcaccagaa ataaaatgat ttttagttct gatgcaaaat taattgctac 
      421 atatttactg aatgcttatg atgtgcaaga aagaagtttt aagacattag ctttgttgga 
      481 atttccatat tttcctgccc ttattccttg atttttcttt gcctctagcc tgaataatgt 
      541 tgatttttca tgaggttgcc acaaggtaag gcaacaacac atggaagaaa atagggcaga 
      601 cacatgcatt cccctgaggt acttgtatag taaccaagaa atcagcagca aggtgttggg 
      661 gggacttaac taggtaggtt gcacatttga ggctatccac tcaactaaca gacagttcca 
      721 ggctttgctg agtcaacacc tttcacagaa gaaagaccat catatatttt atcccacttg 
      781 gcggcagctt acaataaaac acatgcagag aaaatgctta aatataaaag ttcaacagca 
      841 ctatataaaa ggaagggtta attatactag gaatcagata taaaataatt actgagcatg 
      901 ctcttagctc tgagcttcct ggaagccaag caaaaataga accatgatag ttcaaggctg 
      961 ctacatattg atgcatgtag cttcaattgt gaagatggta gcatccccct accccattta 
     1021 acctctcacc tttctctttt gttttatagt tcggcctgat ctaattagtt caatttggat 
     1081 gcttccttga gttttttttg taacatattt tatataaaga agtcagttag tgacaaataa 
     1141 gcagtttgag gagaaatctg ttaatattta ttttgtagcc atcagattta cttcacatag 
     1201 aaaggtcttt gggttgggtt tgaacttcca aactctcaaa ggtaaatgcc acattaacct 
     1261 ttcattaacc aaattcttac accaagctga tagatttggg atgtcctttt tacttctatc 
     1321 ttccataata ttctaaaatt attttccctt gttttgttcc tatcctactt cctcttagtc 
     1381 tactttgttg acttcattaa aaaacaaaaa accagttgtt ggatacttga gctaaactgc 
     1441 cttaaagaat ctgcagattt tattttattt tttttctctc aagagggtaa aaggaagaga 
     1501 gctacaattt ctaagaagcc tggcttggct gtctgagtct ggcccccagg cagattaggc 
     1561 caaggttttg gccaagtgaa attgccaatt ttctaaaaga aagggctagc acattgctca 
     1621 ttagagcatt ctgattttgt ctgcgcaatc tttctgctac cccgcaattt cctgttggtt 
     1681 ataaatgaaa cctttctagc tgttaatgca gcctgtgaat ttttttaaaa gcatgtaatt 
     1741 aatcatagga ggttgggggg attcactaag cctgagttac atgggagaag ctggacaagg 
     1801 cactaggacc tagaaggcat ctatccaccc tggcaggaat ttcttgcttg gagctcagac 
     1861 aacaaaggca tagagagatt ggttttcttt ctctcagcat ctccacccaa ccagcagaaa 
     1921 accggtgagt ggggctttta agtgattttc aagaagaatg taacagatgt caaacgggaa 
     1981 aagcacaagg caaagcctgc tctctctgtc tctctgtctc ctcttctcct tttttgcctt 
     2041 attctatccg attttttccc taagcttcta cctgggattt tcctttggaa aagtgagttt 
     2101 gatgttcctt tgttttcact gtgatgttaa tttagaataa tactacctct gatcctaaag 
     2161 caaagcaaag ccttactggc atgcctgggg aaatgtttgc tgcttgcctt gaggaggtgg 
     2221 ggtctcttac cactgcaggt tgtctgacag agacaatgct gagctcagca taggtcatgg 
     2281 tgacattgga aaaaaggcgg aattgagcct ggcagaccca ttaggcacca gtctttctta 
     2341 tctcctgtcc tcctggtccc ttgcaaatat attgatgtgg cagtgtgtag cagctgagcc 
     2401 ctgcttgctt tgtgagtcct tttatcccca tctgtgagat gcatgttaat agtttggctc 
     2461 gtaggatgtc actacatttg ctagcatttg tggcttcagt tgtattgggt ttcatgtttt 
     2521 gattgtttgg ggttcttggt gggggagggg gttcaacaga agggagaaaa gcaaagcctg 
     2581 acaaatgacc atcttttctc agctaatgca cctgggcaat atacaagttt ggggtgaatt 
     2641 gcctgctgtg agggtaaatg tcacttcaat taaggtagaa acccagaaca atgaaaggtg 
     2701 tgcttccttc taaaggtccc gtatgctgtt cggagagtca tttgtgaatc tttcaacaat 
     2761 taaattattc cattaagagg tgttgctgca tcagtgggga gggggtggag cacctggggg 
     2821 ggaaaaaaaa ggattttgtg aacaaatgga accgggggaa gacagagcta gtaacttgtt 
     2881 aaataactta tttttctaat cctttttccc cccagcttat ttcttatgaa tgtcggatag 
     2941 ctgcaccagc ttggtgggga aagggtttga tgaatagcac aaagacactg gctgttccct 
     3001 ggaggctgtc cctttaaagg agaatcttag tttattctgg ggggagggga tgcacacatt 
     3061 agagtaggaa agagggcttg gaataaaatg aaaacactcc cccttcatag tcattgtact 
     3121 gaaatgcaaa gactgcttcc taagctggag atgctaacct tgggtagctc cttctgttct 
     3181 cttcaagggg aattttgtca ggctatggat tcatttacaa ctgttagtca tgtgggcatg 
     3241 tgtgaggaaa cagatgccag ttttaatgta tttagcccga agttccaatt tgataggagc 
     3301 cactgtcagt aagtctcagg attttcagct atttcaaaat ctccccttct cctctgtctg 
     3361 gaacagtgcc aagagtgcct ccctctctat ctcttactcc caacccccac aaccaccagc 
     3421 acccccgccc agcccctcct tcttctctat taagatcaat attcctgcag gtcaggggca 
     3481 agcagcagat gggtcacagg cttttttcaa ccagttcttt tcacaagcag cagattgcag 
     3541 atctggatct ggctaatatt taaaatccct tcttttttcc ttctccttgt ccctttttgt 
     3601 ttttgcctct cttcaccccc atccctttct cccacgctca ggtctctgag gttccaccgg 
     3661 cgcgccgaag ttcctattct tcaaaaggta taggaacttc cgccaccatg gtgagcaagg 
     3721 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 
     3781 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 
     3841 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 
     3901 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 
     3961 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 
     4021 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 
     4081 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 
     4141 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 
     4201 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 
     4261 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 
     4321 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 
     4381 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagttattt ctgaactgct 
     4441 gcccgggctg ctgcatggaa ccggatccaa aaaagaagag aaaggtagat ccaaaaaaga 
     4501 agagaaaggt agatccaaaa aagaagagaa aggtaatata agaagttcct attctctaga 
     4561 aagtatagga acttcagcgg ccgcgactct agatcataat cagccatacc acatttgtag 
     4621 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 
     4681 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     4741 gcatcacaaa tttcacaaat aaagcatttt tttcactgcg aattctcgta aaactcttca 
     4801 tggcagtagt tattattctc tctctctctc tttttctttt ttcttgagac aggatatttc 
     4861 tctgttgccc aggctggagt gcagtggcac agtcttggct cactgcagcc tggacctcct 
     4921 gggctcaagc catcctccca cctcagcctc ccaagtagct ggggctacag gcacatggcc 
     4981 accaggccca gataattttt catttttgta gagactgagt ctcaccatgt tacccaggtt 
     5041 tattattctc attttttaga tgaagagact gaggtccaga gaagctcaat gacttgccta 
     5101 gttttacaaa tctcctgcca tcacataccc ctcagcgtcc ttaataagag ggaggccacc 
     5161 aactatgtgc tgggcactgt ggtggatgct ggagctatag ggttgagtat ataagaaatg 
     5221 gtgttgctgg agcaactgtt gcttgcttac ctgacctatc tgagaattaa ttagcagggg 
     5281 aacatatttt tgttttcaga ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt 
     5341 agtaataaca gtagttccca tttgctgact gtactgtcct aagtgcatat atatatacat 
     5401 acacacacgc atacctatac tcctctaata ctcaaaatga tcctgtttat gtattgttaa 
     5461 tatgctcatt ttatttttaa atttttattt atttttattt ttatttattt ttgagacgga 
     5521 gtctcattct gtcgcggagg ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc 
     5581 gcctcccggg ttcaagtgat tctcctgcct cagcctccgg actagctggg attacaggcg 
     5641 cccgcctcca cgcccagcta atttttgtat ttttagtaga gatggggttt cgccatgttg 
     5701 gccaggctgg tctcgtactc ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg 
     5761 ctgggattac aggcatgagc caccgcgccg ggctaatatg ctcattttag tgaggcaaaa 
     5821 atagaggctc agagtctgat ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg 
     5881 tgtaccctga tctgggtgac tctaggactc taggtctcaa ctgttacaac cagttaaggg 
     5941 tttggggaag cactgggcca agagtcagga aaatggaagc cacaggtagt gcaaggtctt 
     6001 gggaatggga cgtctggtcc aaggattcac gcgatgactg gaacccgaag agccggggcc 
     6061 cggtttacgg ccgccatgaa gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct 
     6121 gaataggaga ctgagttggg agggaaaggg gcttcgctgg gggagcctcg gcttcttctg 
     6181 ggagaaaatt cccacggcta cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc 
     6241 gcgggcgggg ccgggggcgg ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct 
     6301 gcttctcctc agcttcaggc ggctgcgacg agccctcagg cgaacctctc ggctttcccg 
     6361 cgcggcgccg cctcttgctg cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc 
     6421 tcctgagcag tcagcccgcg cgccggccgg ctccgttatg gcgacccgca gccctggcgt 
     6481 cgtggtgagc agctcggcct gccggccctg gccggttcag gcccacgcgg caggtggcgg 
     6541 ccgggccctg aggcgcggga tcgatccatg acaccccccc gccccccata tctcactata 
     6601 attcactgtc aaatggcttt tatgatttgg agtttgggga tacttttaaa gacagggtct 
     6661 tggtagctcc tagatggctt gggattcact tagtaaccct tgctggccta aaattcagga 
     6721 acttctacct catcttccca agtactgggt tagtagggat gtccagcata tttagcttgc 
     6781 tttaatgatt ttgctgtact tttttctgtg tgtacaggtg catgtatatt tataaaacta 
     6841 acataatata ttttaccccc aaagtaccat aaccctaatt aatgctatta aatgttttgt 
     6901 atgctgggct ctgggcatac taatataacc aatattatac aatgcaaaac aattttggaa 
     6961 tatcttctgt aaaattatct tattagtaag ttgtaaccat ctcagacttc taagtttgag 
     7021 tttgtaaagg atcagttttt tttggtgacc tacacacaca cacacacaca cacacacaca 
     7081 cacacacaca ggggggtgga ttctgagaat ttagagaatt tagatggtta ataccagttt 
     7141 ttatttttat tctgtgtgat atttatttat taagatttat ttatttatta catgtaggta 
     7201 cactgtatct gtcttcagac acaccagaag agggcatcag atctcgttac agatggttgt 
     7261 gagtcaccat gtggttgctg ggatttgaac tcaggacctc tggaagaaca gtcagtgctc 
     7321 ttaaccgttg agccatctca ccagccccgt gatattttat ttaacttact agaatatact 
     7381 gtgttaaaac atattttagg ggattgggga gatggcccat tctgtaaagt gtctgccatg 
     7441 tcatcttcag gacctgcgtt tgtaacccca gcatggagaa gacagagaca gggagacccc 
     7501 tgggcttcct ggcaagctag actagcaaat ttggttaact aggttcaatg agagactcag 
     7561 tctcaaaaca taagaagtga attttttaga cccaatttca atctctgaac tccatacgta 
     7621 tgcacatgta cctgcatata aatatatata cacacccata aacacatgca cacacaaaaa 
     7681 atggttcaaa agttcttaaa atgtataaga gtagaaagtg tagagccggg agtggtggcg 
     7741 cacgccttta atcccagcac ttgggagcag aggcaggtgg atttctgagt tcgaggccag 
     7801 cctggtctac agagtgagtt ccaggacagc cagagctata cagagaaatc ctgcctcaaa 
     7861 aaaccaaaaa taaagtgtat atactaatct ttcatgccta aaatatttat ttttgccaca 
     7921 tttttcatac ctctttaata tttttctttt tctctttgtg cttcaaagga aattttgtgt 
     7981 catgttttac ttttcattct tgactctatt cagcagtaag acgcagcatg gctttcttag 
     8041 agaagtataa tctattacaa aacggtaaca tttaactgct ctacataccc tcttgtagct 
     8101 aaattcctct gttaaactaa gacttattat ctaaacgtaa ttaaatgttg atatcttata 
     8161 atgctccact ttgaaacagc tgtctttctt ttgcagatta gcgatgatga accaggttat 
     8221 gacctagatt tgttttgtat acctaatcat tatgccgagg atttggaaaa agtgtttatt 
     8281 cctcatggac tgattatgga caggttagta agacctcgat tgaagttatt tttagagggt 
     8341 gtgtgtgtgg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag 
     8401 gagaggcatg tgtgatatat atgtgaagtg tattgcatgt acatggaatc cagagaagga 
     8461 cattaggtgt cctactctgt tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc 
     8521 tggggcatac atactgattt ggctaggctg cctggctagc aagacccagg gatctgcttg 
     8581 tctctgcccc ccagtgctga cgttacaagc aggtgcaacc acatctggtt tttatatggg 
     8641 tactggggga tctgaactta gggcctgatg cgtgcccaag tgctctcacc cgccaagcca 
     8701 cctctccagc caaagggttt tttttcttgt attaattcta acttagcaac tgttgagtag 
     8761 ttgatgtttg aatataagct aagccaactt attagaataa atgttttcta ttttttactt 
     8821 gtatgtgtga gtgcctgcat gtatgtatgt gaagcatatg tattccagga tgtcagaatt 
     8881 gtgatggatt ccctggaact ggagttacag accattgtga accactgtgt atgggtgctg 
     8941 ggaactgaac tttaggtcct ctgcaaaaac agtaaggatc ttaactgata aactatcttt 
     9001 ctagccctaa ggctttctac ttttaaagtt cgtttctctt tctctctctc tctctctctc 
     9061 tctctctctc tctccctctc cctccctcct cctcctcctc ctctttcccc cctctcgcct 
     9121 cctctttctc tctcccctcc ctctctctct ctcacacaca cacacagaca gagagaggag 
     9181 agagaaagga agccttacta attaattacc tagttttaga cataactagt gggaatccat 
     9241 agtaagtaaa tcaacattct tgaagagagg ttagaagaat tggctctcgt tgtccatcga 
     9301 ggctgtataa gattattctt ctgttttctc agcttaccat acatattttc tacgtagaca 
     9361 ataccaattc aaatacactg tacgtaaagt atatgcgcac acacacacac acacacacac 
     9421 acacacacac acaagttaca tattgtcata tagtactaaa atttatggac tttttcatat 
     9481 taatacatct agagctatca tgttttttat cttttaaaaa ttactttacg gggctacaga 
     9541 gatgtctcag cagttaggag cactgactgc tcttccagag gtcctgagtt caattcccag 
     9601 caaccacatg gtggctcaca accatctgta atgggatctg atccactctt atggtgtgtc 
     9661 tgaagacagt gacagtgtac tcatatacat gaaataaatc tttaaaaaat tactttacca 
     9721 tcagggtata tatatatata tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc 
     9781 tatttgagag ggtctcactg tttagctcag tctggcttgg aacgttctgt atagcccaga 
     9841 ctggcctcaa ctcagcagtc cccctgaaag gctctcatat gtggagatta caggtatgtg 
     9901 ctactgtgcc cagccctaca tgttactttg tttttgaaat agggtatcaa atagcttaga 
     9961 ctggcttcta agtcagtgtg tagctaagga tggtctgagc ttctgaacct gctacttcaa 
    10021 ctcctggtgt gccatcatgg ccagtttgca cagttgtgag agtagaactc aggacctcct 
    10081 atgtgttact agacatgttc tctaccagct gagctacatc ccaattcgcc tacatttact 
    10141 tttatttttt aaaagattta tttatgtata cagcactctg cctgcatgta catctgcatg 
    10201 ccagaagagg gcaccagatc tcatcataca tagttgtgag ccaccatgtg gttgctgggg 
    10261 attgaactca gggcctctgg aagagcagtc agtgctctta acctctgagc catctctgta 
    10321 gccccctaca tttattttaa agattatagg ctattctatt catagatgtg ccataactga 
    10381 tttaaccaga tcttcattat tttgtactta gtagagtata ttagagggaa tgaatcttaa 
    10441 atacacattt tgacacttgc tcagctttag acaagtaaaa atcactggtc aaggaaatgg 
    10501 tgcttagtgg atgcttctga actgtcaaat tgtcctccaa caagttacat cattttatac 
    10561 ttccaacgtt atgtgagagt tcatttttct ttaggctctt ctagtatttt atatttgaga 
    10621 aataaaattt aaccaaaatt tgccaatcta gtaagcttgt ttttatttgt atctttaaat 
    10681 tttatgatag atgttatagt gtactctcct ctcccctccc ctcccctctc ctcctcagca 
    10741 ggatcttgtt atgtagccct aagggatcta atacttactc tgtagtccag gctgtccttc 
    10801 agctcatgag acacaccatc atgcttggca tgtttcttgt tctgaaaggt atctggtttt 
    10861 aattttggtt gagctggtgt gttggtgtat atctgtaatc tcagcacagg caagtagaga 
    10921 caaggattat gattacagtc cagcctgggc tatttagagg gattctcttt aatctttcct 
    10981 catgccccaa aatcttacct ttggtatatg aaaaatagtc tccacttctg caaaatattg 
    11041 ctttatgaag taagaattaa ttcccttcat agagacaagg aatgtgtcct gtaaaagttt 
    11101 aatgtgtaag aagtatttgt tataaaagat aaatattcag aatcttcttt ttaattcctg 
    11161 attttatttc tataggactg aaagacttgc tcgagatgtc atgaaggaga tgggaggcca 
    11221 tcacattgtg gccctctgtg tgctcaaggg gggctataag ttctttgctg acctgctgga 
    11281 ttacattaaa gcactgaata gaaatagtga tagatccatt cctatgactg tagattttat 
    11341 cagactgaag agctactgtg taagtataat taacttataa ttaaaaaaat agggccattc 
    11401 tagttttatc tatatttttt ttaaacttgt gcaaactatg ctacataatc aacttacttg 
    11461 attatgtaat atatatagta atagttggaa ttactattat tacatattta tttttctgtg 
    11521 ctggggtaga acccagggaa ctaagtactg taataaccga gcttcattca tcctagtcat 
    11581 ttttaatttt tttttttttt tttgtttttt cgagacaagg tttctctgtg tagccctggc 
    11641 tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagaaatct gcctgcctct 
    11701 gcctcccagt gctgagatta atgacgtgca ccaccactgc ctggcatttt tttaaagttt 
    11761 taatttgaga ccctttctca ctaacttgct cacactatcc tcgaactcat tacgtgggct 
    11821 ggtctagaaa tttcagtcaa cttgcctcag cttctcaagt agctgggatt acaggcaggt 
    11881 gctaccactt ctggccatga tcattgttgt tgttgctatt attattatta ttatttcact 
    11941 gatacttcat atcacaacac agaatagtaa ctgtagttca tgaccaacaa aaaagttgta 
    12001 cagtttctct gttgtcttat tttgatctgg attttgattt gtttcatggc actgggcagg 
    12061 ctaatctcaa actgcatggc tcaaatagtc cttctgtctt aatctcccat gttctggaag 
    12121 tataggtatc actacaccta gctgtatttt catattttaa aaaaaacgcc taagaattgc 
    12181 tattgaatga ggcggagaga tggctcagca gtcacaagta cttgtagagg actggggttt 
    12241 ggttactagc acctcaaaga tggcttacaa ttatctgtag ctccaattcc agaggatctg 
    12301 tgtggtaaag ctgcatgtat atgggcatgc aggtaaaact gctaaacata taaaaatagc 
    12361 tcagtcttaa gagaattggt cgactttttc gccctttgac gttggagtcc acgttcttta 
    12421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
    12481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
    12541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 
    12601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 
    12661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 
    12721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 
    12781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 
    12841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 
    12901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 
    12961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 
    13021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 
    13081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 
    13141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 
    13201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 
    13261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 
    13321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 
    13381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 
    13441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 
    13501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 
    13561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 
    13621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
    13681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
    13741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
    13801 accaggtcga gtagggataa cagggtaatg gatcctcgtt gggcacctgc actcatgtac 
    13861 gcaggcccac atatagatgt acacacgcat atcattcaaa atagaaatta tttatacaag 
    13921 tagtgcgttg gagagctggc tcaggagttg ttaatgcttg ctacccttgc aaaggaccca 
    13981 agttcaattt ccagcacctg tgttgggcat ctcacaacca catgtaaatc tagtttcagg 
    14041 ggatttcacc cctcttctga actcttcagg cacttatacc cacaaatgga catctccaca 
    14101 tagagacaca tacttttaaa aaagtaatct agggtctaga gaaatggtta tagaccacgg 
    14161 actgggatca gttcccagaa gccacatcct gtggctcaca attgcctgtg aatcaagttc 
    14221 tagatctggt ggcctcgtct agcctgcagt catcaacaaa cacatgcaca catccataca 
    14281 cacataattt aaaagaaagc gaaagtaacc tataatgcta aacaaagcac attctcagtg 
    14341 tagactgcag tggtgatgaa ccttgaaaat atattaatag aagccagaca cgtaagtcac 
    14401 aaatggtata actcaattta tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa 
    14461 aggcagatta ttggttgtca gtagctacag agatgggaca ttgaagatat taataggtat 
    14521 agggttgagg attcatggcc tttccctctt ccatgttagc atgtctattg ttgttaacct 
    14581 tattcaactc atgttagggc aatcatattg atgagactta atgagtatag cttgacattc 
    14641 ctagaagaca gagtctcaca tcagactctc tgatcctctg actcttagaa tctttcaaca 
    14701 ccctcttctg aaatgatctc tgagcctttg gttgtgactg aattatgaga aagaatttta 
    14761 tgttgaatga attttacttc agcattttta caaaactgaa gctggggatg gcgtttcatg 
    14821 cgtgcagtcc ctaccatttg cctataagcc tggaggaggc agaagtagaa ttaggctttt 
    14881 cagggacatc ttcagctgca tcatgagctt gaggacaact tgagctacat aagattccca 
    14941 tccccacccc cacacacaca cacacatcta gtatgtacct agtatgcagt cttttcatat 
    15001 gtttaaaatg tagaaaggta tatgtgaagt ctcccttttc cccacaactt agtccctcca 
    15061 aagtattgca tcagtttcct tgtacatgat tttatgcata tataagatac actatttctc 
    15121 tctctctctc tctccttccc tccttccctc caacctctgg gtatgagttg agggttatgt 
    15181 atgtttcaag ttgtaataag aattgccact caaacatttc atcccatttt aagttaagtt 
    15241 tgtctgattt tccactttag aagggcagcc atgatggctc atgcctataa tcccatccta 
    15301 gcacttgtaa gcctaataca taagactgga ggattgtcat cagcttgagg ctagcctggt 
    15361 ctacctaatg agttccagcc agccaagagt agaatataag accctgtctc caaaaaatgt 
    15421 accaaagggg ctggaggggt aactcagtgg ttaagaacag tagctgctct tccagaggac 
    15481 ctgggttcaa ttcccagcac ccacatggca gcttgcaact atctgtaact gtccagcttt 
    15541 gggatatccg acaccattat accaattaca taaaatgaaa aaattgtaaa atgtgctaaa 
    15601 atgagtgggg agggggagca tggtgtggtt ctgtgggctt gggattttgc catgaatcca 
    15661 tgactatatc tcagcaatat gtggattatt tctggaaacc tgtggggcat gggtgtatgg 
    15721 gaagagggga gagagagaga gcccgcatcc agccagagtt cctgtgctct gggcaggcgg 
    15781 acgtgggagg actgctggac gatttccact tggccctggg tgggcatctg gctgtgtgaa 
    15841 gccactgacc ccactcagcg gggggtagat aaggggcatt tcccatacca ggttctcaga 
    15901 ctccagcatt ccacgctgga tacagcagaa gagctgagaa cagaataggg gccccaggga 
    15961 gacactctcg tcccagagat aaagaaggca gagggagaga gattcctacg caggcgagag 
    16021 gccttagtct gggccttgat gaacagagac ggtctatggt tttagagctt tattgtagaa 
    16081 aggcaggggg aaagagagaa ggtagaaaga gagagaccag ccatggccaa gaggagagaa 
    16141 ggggggaaag gagagagaga agaaaggcta gagagtaaga aaggttaaag agacagaagt 
    16201 aaaagcttag agtaagagag agagagagag caagagggag agtaagaaaa gtaaaagctt 
    16261 aagagagcga ggaggggcca gcccctctaa tagtgggctt gttatcttgt tgttgctagg 
    16321 taactgtgaa gcgtctagcc tgaaggccag aagcttggga cattgtctat gtgactgaaa 
    16381 gccatgagcc tctactgtgg gggctgtgga ggtggtaact tggacaggag ccaggggttc 
    16441 caggagacat gagggaatgc cttctgtccc atgtaggtag gaattaccac caccggcttt 
    16501 caccgtagtt cactggagac tgtttgtaca tagcccattg ccccacagaa accaggcact 
    16561 cactgtttca tttgcataag aattagaagg ctttattttg gctgggattg gtgacttgcc 
    16621 ccagaaattc cagcttttgg gaagccgaat cctggagaac caggagttca aggtcatcct 
    16681 caggtacctc atttgttcaa gaccagcctg tgctaccatg agaccttgtc cctactcccc 
    16741 tcaaccccaa aacacgctgt tttcatttta taggttgtac taaccatgga ggatggtcat 
    16801 ttgcctttct cttgatttca gaagcgtagc gcgggctttg caccatgatc tagtgtttcc 
    16861 agataggctt gcctctccac agccaggctt atcctgcttc agtcccatgt ttggcaaggg 
    16921 caacatatga ctgaagaact tgcatagcct tatggtgtat catgactctt agccccagtt 
    16981 tataactgtc taacagctgg tggcttttgt gaaaaaggaa agcatcattt gaacttttaa 
    17041 cttttcagct gtttgttttg actttgcata aatactgaga ctagctagga aatgagcttt 
    17101 tgtttcattt gctaaaaatg attttttttt catgaggcaa tttcaagcaa tttgaattta 
    17161 gcaatctcta cttgtctgaa aagaagttat taattaaaaa aaaacaaaca aacaggaacc 
    17221 tattatgctg gctagtcaca tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca 
    17281 catgaaatta ttttctaatt ccccaaatta catttatgct aattagtatg tagtaaaaca 
    17341 ttttaattgt ttccaatgtt aaacattaca gaaataattt tgatgttttc ctaataaccc 
    17401 agcctttgat catgtttgca tttatttgat tgttgtcagt ttattaaata attaagtaag 
    17461 agcaacaaga taaaatgact tcatgagagt ccttttctgt aacttagaac gtcagtagtc 
    17521 ataggaactg cggtcgttcc cttacctttc agaagtaggc taaagagttg aacgcaaagg 
    17581 tggtttttcg aatcttcctg atccttaagc ccccactagg tttatattct atatttgata 
    17641 tcggattcga acccctcgag cttaggatcc ggaaccctta atataactaa gttattaggt 
    17701 ccctcgaaga ggttcactag tactggccat tgcggccgct tcgaggctgc ctcgcgcgtt 
    17761 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
    17821 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
    17881 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 
    17941 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
    18001 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 
    18061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
    18121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
    18181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
    18241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
    18301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
    18361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
    18421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
    18481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
    18541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
    18601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
    18661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
    18721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
    18781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
    18841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
    18901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
    18961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
    19021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
    19081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
    19141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
    19201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
    19261 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 
    19321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
    19381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
    19441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
    19501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
    19561 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
    19621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
    19681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
    19741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
    19801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
    19861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
    19921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgg 
//
