LOCUS       Ple104\(pEMS1368)       18832 bp    DNA     circular     31-JAN-2011
SOURCE      
  ORGANISM  
COMMENT     NOTE: Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|580573466|
COMMENT     VNTDBDATE|580573466|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple104 (pEMS1368)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|George Yang|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "Ple104 (pEMS1368)" 0 18832 
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COMMENT             (CStringList) 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 20 20 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "13896..17707") 
COMMENT        #3=(CFSignal (CObList) "AmpR" 22 0 0 17825 18825 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "19027..20027") 
COMMENT        #4=(CFSignal (CObList) "AmpR" 22 0 0 11222 12668 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "12424..13870") 
COMMENT        #5=(CFSignal (CObList) "3' arm of Hprt homology" 86 0 0 5405 11226 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6607..12428") 
COMMENT        #6=(CFSignal (CObList) "HPRT complementary sequence" 86 0 0 3629 5404 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4831..6606") 
COMMENT        #7=(CFSignal (CObList) "MCS" 21 0 0 143 173 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "143..173") 
COMMENT        #8=(CFSignal (CObList) "FRT (F5 mutant)" 21 0 0 2511 2544 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3713..3746") 
COMMENT        #9=(CFSignal (CObList) "NLS" 21 0 0 3308 3380 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4510..4582") 
COMMENT        #10=(CFSignal (CObList) "FRT" 21 0 0 3386 3419 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4588..4621") 
COMMENT        #11=(CFSignal (CObList) "SV40 early polyA signal" 21 0 0 3573 3623 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4775..4825") 
COMMENT        #12=(CFSignal (CObList) "EGFP (mutated stop TAA -> TTA)" 21 0 0 2552 
COMMENT             3271 0 (CStringList) (CStringList) 1 1 1 1 "3754..4473") 
COMMENT        #13=(CFSignal (CObList) "Kozak consensus" 21 0 0 2545 2555 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3747..3757") 
COMMENT        #14=(CFSignal (CObList) "Tetracysteine residue" 21 0 0 3272 3307 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4474..4509") 
COMMENT        #15=(CFSignal (CObList) "I-Sce I" 21 0 0 12675 12692 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "Ple104 (HAP1-B)" 21 0 0 174 2502 0 
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COMMENT        #18=(CFSignal (CObList) "oEMS2380" 27 0 0 1888 1909 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: George Yang" 
COMMENT                          1) 
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COMMENT                          "Created: 01/31/11 02:24PM" 1) 
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COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                   "Tetracysteine residue" "@N" 1 16.3925 0 0 
COMMENT                                   -10 10.0405 1.17389 #179#) 
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COMMENT                                   -10 7.49292 1.17389 #183#) 
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COMMENT                                   -4.09633 12.1963 0 -10 2.87229 1.17389 #188#) 
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COMMENT                                   1 10.8535 8.71165 0 -10 4.79547 1.17389 #193#) 
COMMENT                             #195=(CScratch 
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COMMENT                            (CObjectList)) 
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COMMENT                                   "5' arm of Hprt homology" "@N" 1 -16.8739 
COMMENT                                   1.74233 0 -10 11.2144 1.17389 #198#) 
COMMENT                             #200=(CWideArrow 
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COMMENT                                   "3' arm of Hprt homology" "@N" 1 11.4827 
COMMENT                                   -10.454 0 -10 11.2144 1.17389 #200#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.809 
COMMENT                                   -6.96932 0 -10 14.9609 1.17389 #202#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #204=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -186 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #205=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #206=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1119 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1953066364 
COMMENT       (CStringList) 1869112175 1769104494 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     12694..16505
                     /vntifkey="86"
                     /label=5'\arm\of\Hprt\homology
                     /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263"
     misc_marker     17825..18825
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     11222..12668
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     5405..11226
                     /vntifkey="86"
                     /label=3'\arm\of\Hprt\homology
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3629..5404
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2"
     misc_feature    143..173
                     /vntifkey="21"
                     /label=MCS
                     /note="New MCS compared to pEMS1040."
     misc_feature    2511..2544
                     /vntifkey="21"
                     /label=FRT\(F5\mutant)
     misc_feature    3308..3380
                     /vntifkey="21"
                     /label=NLS
     misc_feature    3386..3419
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3573..3623
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    2552..3271
                     /vntifkey="21"
                     /label=EGFP\(mutated\stop\TAA\->\TTA)
     misc_feature    2545..2555
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    3272..3307
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    12675..12692
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    174..2502
                     /vntifkey="21"
                     /label=Ple104\(HAP1-B)
     misc_feature    1626..2502
                     /vntifkey="21"
                     /label=Prom\region
     primer          1888..1909
                     /vntifkey="27"
                     /label=oEMS2380
     primer          1359..1378
                     /vntifkey="27"
                     /label=oEMS2400
BASE COUNT     4881 a      4343 c      4423 g      5184 t         1 others
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatataac 
      121 ttcgtataat gtatgcgaat tcgagctcct aggccggcca cgcgtggcgc gccaagttaa 
      181 aggggaacct ctgagggcct aggccctcaa gtcccccatt ctctgctgat gtaaggaggg 
      241 actgtacctg ggttctgggg tgggggtgag ggctatggcg gccactgcct ggttctggag 
      301 gggaaggcct ggatcagaga tgggggaggg cccccgaaag gcctgcagga gacagcagga 
      361 ctgcggcggg ggtgaggagg tctcccctgt tcctagttca gggtgcccct ccaccctgct 
      421 cccctgcaga aagtaacccc ggtgcctgtc ccccccaggt ccctggcacc gacccttagg 
      481 ggctctttct attacctacc ctgttccagg tgtttttttt cccagggtca ggccagggga 
      541 gggggcagga ggaggggcct gagccacctg tgtgctgccc agggctgggc actggagtga 
      601 atgtgcccca ggttcagggg gtgtgcacaa ttaggtggtg ggtggtctgg ctttgcagcc 
      661 tggcaagtct tctgcaccct cccatcctct ttcggatcca ggagggtcca tggatgcaca 
      721 ctgggaggga ggacaggagg ccaagggtct ttgctgtgtc tgatttctcc ccctagatga 
      781 gaagcaggag agggaggggg tggttgacag aggaaaacca cttgagaggc ctggatcaga 
      841 gactaccccc agtctcctgg acagccctag gctgtcgaaa ggcagatatc tgtcagccag 
      901 gccaaaatgc ttacttccag aatgacgggc cctgagccct ggtttctcca tttgtaaaat 
      961 ggagtctctg tccctactcc actttctgtg gggcgactga gcctgtaggt gaggaagcca 
     1021 cgtggggagg tatacgggct gacactgccc tctggtggcc gtagccagag ttgctggctg 
     1081 ccagtgcgct gacctagagg gtgtggtcag ctgggggagc tttcagggtc tcctaacttc 
     1141 atctctgtct tctccatccc actgcctctg ccttcttccc cgtcaaactc tccaggggtt 
     1201 tcccagggct tcttcctcat ggggccagaa acaatttaat tcagcaggag tgtaataggc 
     1261 ttcttctgta tgccctgttc tgtgcttggt ggttggggct gcagagatgt ccaaggcaca 
     1321 gagagacagt ccaggaagct gggctgtggg aggaccactc ggcagagcct atggagctgt 
     1381 agcctcacgg aagcctgggc tgtgccgatg cccgaacagc cacctcctta tgttcttcaa 
     1441 cctcactgtc gtagcaaacc ctggggttgt gtgtctcctt caagctttct gctgacaaag 
     1501 aaaccatagg aaacatgcag actttgggct gggcacagtg gctcacgcct gtaatcccag 
     1561 cactttggga gaccaagggg gaggcggatc acgtgaggtc gggagttcga gaccagccta 
     1621 accaacacac gccaccatgc ttggctaatt tttgtatttt tagtagagac agggtttcac 
     1681 caggtggcca ggcttgtctg gaactcctga cctcaggtga ttcgcctgcc ccggcctccc 
     1741 aaagtgctgg gattacaggc gtgagccact gcgcccggct acagggagac ttttaagagg 
     1801 cgtctcccaa ggagaacagg ggttcgccag catggcaaga ggagggcaaa gcgaactagg 
     1861 gttggaggtg aagaacagtt aatccaatca aatggaagac gtcgagtggc gcgagcactg 
     1921 gggtgtgagc gcgagggtca gggttgcagc gccaggcagg gttgcagcgc caggcagggt 
     1981 tgcagcgcca ggcagggttg cagcgccagg cagggttgca gcacgttgga agcgcactgg 
     2041 agtttggaat gtctctccca gtcaaccggg aaagacgcag cgctgcccta atgcactggc 
     2101 caccccagac cgtatgcaca acgttgggtt ggcagcttct acaccaggga aggcacccga 
     2161 aaccccggcg cggcagctgg ggaggggctg cccccagcca gggacccgcg agtgctgggg 
     2221 cagccgcggc tttgggcaga accattctcc ggagatggag agggcagaaa aaatctttcg 
     2281 ctcccatccc aactcggagc catccggaac ttgcactcgc ccttcttgcc ctgcttttct 
     2341 tactgtgctc tgttccgtca ccactagctc ccctcccgcg cggctctgag tcccgcttgc 
     2401 cactagttgc ttctcggcag cccccacccc tacgcgtggt ccggcccggc ggctggcgca 
     2461 ggttataaag gtcccgccgc agcagcggac ggcagactcg agggcgcgcc gaagttccta 
     2521 ttcttcaaaa ggtataggaa cttccgccac catggtgagc aagggcgagg agctgttcac 
     2581 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 
     2641 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 
     2701 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 
     2761 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 
     2821 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 
     2881 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 
     2941 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 
     3001 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 
     3061 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 
     3121 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 
     3181 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 
     3241 cactctcggc atggacgagc tgtacaagtt atttctgaac tgctgcccgg gctgctgcat 
     3301 ggaaccggat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc 
     3361 aaaaaagaag agaaaggtaa tataagaagt tcctattctc tagaaagtat aggaacttca 
     3421 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt 
     3481 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 
     3541 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     3601 aaataaagca tttttttcac tgcgaattct cgtaaaactc ttcatggcag tagttattat 
     3661 tctctctctc tctctttttc ttttttcttg agacaggata tttctctgtt gcccaggctg 
     3721 gagtgcagtg gcacagtctt ggctcactgc agcctggacc tcctgggctc aagccatcct 
     3781 cccacctcag cctcccaagt agctggggct acaggcacat ggccaccagg ccagataatt 
     3841 tttcattttt gtagagactg agtctcacca tgttacccag gtttattatt ctcatttttt 
     3901 agatgaagag actgaggtcc agagaagctc aatgacttgc ctagttttac aaatctcctg 
     3961 ccatcacata cccctcagcg tccttaataa gagggaggcc accaactatg tgctgggcac 
     4021 tgtggtggat gctggagcta tagggttgag tatataagaa atggtgttgc tggagcaact 
     4081 gttgcttgct tacctgacct atctgagaat taattagcag gggaacatat ttttgttttc 
     4141 agattcaata taagaacttg tgtgggcaaa aataaagatc agtagtaata acagtagttc 
     4201 ccatttgctg actgtactgt cctaagtgca tatatatata catacacaca cgcataccta 
     4261 tactcctcta atactcaaaa tgatcctgtt tatgtattgt taatatgctc attttatttt 
     4321 taaattttta tttattttta tttttattta tttttgagac ggagtctcat tctgtcgcgg 
     4381 aggctgaagt gcagtggtgc gatctcagct cagtgcgacc tccgcctccc gggttcaagt 
     4441 gattctcctg cctcagctcc ggactagctg ggattacagg cgcccgcctc cacgcccagc 
     4501 taatttttgt atttttagta gagatggggt ttcgccatgt tggccaggct ggtctcgtac 
     4561 tcctgacctt gagtgatcca cctgcctcgg cctcccaaag tgctgggatt acaggcatga 
     4621 gccaccgcgc cgggctaata tgctcatttt agtgaggcaa aaatagaggc tcagagtctg 
     4681 atttgtacaa aactacagag cagttaagtg tcctctcaga tgtgtaccct gatctgggtg 
     4741 actctaggac tctaggtctc aactgttaca accagttaag ggtttgggga agcactgggc 
     4801 caagagtcag gaaaatggaa gccacaggta gtgcaaggtc ttgggaatgg gacgtctggt 
     4861 ccaaggattc acgcgatgac tggaacccga agagccgggg cccggtttac ggccgccatg 
     4921 aagcaacgcg cgccggtagg tttgggaatc agggagccct ctgaatagga gactgagttg 
     4981 ggagggaaag gggcttcgct gggggagcct cggcttcttc tgggagaaaa ttcccacggc 
     5041 tacctagtga gcctgcaaac tggtaggcgc cggcgtaggc gcgcgggcgg ggccgggggc 
     5101 ggggcctgcg gggcgtggcg gggcgggcag agggcggggc ctgcttctcc tcagcttcag 
     5161 gcggctgcga cgagccctca ggcgaacctc tcggctttcc cgcgcggcgc cgcctcttgc 
     5221 tgcgcctccg cctcctcctc tgctccgcca ccggcttcct cctcctgagc agtcagcccg 
     5281 cgcgccggcc ggctccgtta tggcgacccg cagccctggc gtcgtggtga gcagctcggc 
     5341 ctgccggccc tggccggttc aggcccacgc ggcaggtggc ggccgggccc tgaggcgcgg 
     5401 gatcgatcca tgacaccccc ccgcccccca tatctcacta taattcactg tcaaatggct 
     5461 tttatgattt ggagtttggg gatactttta aagacagggt cttggtagct cctagatggc 
     5521 ttgggattca cttagtaacc cttgctggcc taaaattcag gaacttctac ctcatcttcc 
     5581 caagtactgg gttagtaggg atgtccagca tatttagctt gctttaatga ttttgctgta 
     5641 cttttttctg tgtgtacagg tgcatgtata tttataaaac taacataata tattttaccc 
     5701 ccaaagtacc ataaccctaa ttaatgctat taaatgtttt gtatgctggg ctctgggcat 
     5761 actaatataa ccaatattat acaatgcaaa acaattttgg aatatcttct gtaaaattat 
     5821 cttattagta agttgtaacc atctcagact tctaagtttg agtttgtaaa ggatcagttt 
     5881 tttttggtga cctacacaca cacacacaca cacacacaca cacacacaca caggggggtg 
     5941 gattctgaga atttagagaa tttagatggt taataccagt ttttattttt attctgtgtg 
     6001 atatttattt attaagattt atttatttat tacatgtagg tacactgtat ctgtcttcag 
     6061 acacaccaga agagggcatc agatctcgtt acagatggtt gtgagtcacc atgtggttgc 
     6121 tgggatttga actcaggacc tctggaagaa cagtcagtgc tcttaaccgt tgagccatct 
     6181 caccagcccc gtgatatttt atttaactta ctagaatata ctgtgttaaa acatatttta 
     6241 ggggattggg gagatggccc attctgtaaa gtgtctgcca tgtcatcttc aggacctgcg 
     6301 tttgtaaccc cagcatggag aagacagaga cagggagacc cctgggcttc ctggcaagct 
     6361 agactagcaa atttggttaa ctaggttcaa tgagagactc agtctcaaaa cataagaagt 
     6421 gaatttttta gacccaattt caatctctga actccatacg tatgcacatg tacctgcata 
     6481 taaatatata tacacaccca taaacacatg cacacacaaa aaatggttca aaagttctta 
     6541 aaatgtataa gagtagaaag tgtagagccg ggagtggtgg cgcacgcctt taatcccagc 
     6601 acttgggagc agaggcaggt ggatttctga gttcgaggcc agcctggtct acagagtgag 
     6661 ttccaggaca gccagagcta tacagagaaa tcctgcctca aaaaaccaaa aataaagtgt 
     6721 atatactaat ctttcatgcc taaaatattt atttttgcca catttttcat acctctttaa 
     6781 tatttttctt tttctctttg tgcttcaaag gaaattttgt gtcatgtttt acttttcatt 
     6841 cttgactcta ttcagcagta agacgcagca tggctttctt agagaagtat aatctattac 
     6901 aaaacggtaa catttaactg ctctacatac cctcttgtag ctaaattcct ctgttaaact 
     6961 aagacttatt atctaaacgt aattaaatgt tgatatctta taatgctcca ctttgaaaca 
     7021 gctgtctttc ttttgcagat tagcgatgat gaaccaggtt atgacctaga tttgttttgt 
     7081 atacctaatc attatgccga ggatttggaa aaagtgttta ttcctcatgg actgattatg 
     7141 gacaggttag taagacctcg attgaagtta tttttagagg gtgtgtgtgt ggtgtgtgtg 
     7201 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taagagagag aggagaggca tgtgtgatat 
     7261 atatgtgaag tgtattgcat gtacatggaa tccagagaag gacattaggt gtcctactct 
     7321 gttgctctct tattcccttg aatcagtgtc tcttgctgaa cctggggcat acatactgat 
     7381 ttggctaggc tgcctggcta gcaagaccca gggatctgct tgtctctgcc ccccagtgct 
     7441 gacgttacaa gcaggtgcaa ccacatctgg tttttatatg ggtactgggg gatctgaact 
     7501 tagggcctga tgcgtgccca agtgctctca cccgccaagc cacctctcca gccaaagggt 
     7561 tttttttctt gtattaattc taacttagca actgttgagt agttgatgtt tgaatataag 
     7621 ctaagccaac ttattagaat aaatgttttc tattttttac ttgtatgtgt gagtgcctgc 
     7681 atgtatgtat gtgaagcata tgtattccag gatgtcagaa ttgtgatgga ttccctggaa 
     7741 ctggagttac agaccattgt gaaccactgt gtatgggtgc tgggaactga actttaggtc 
     7801 ctctgcaaaa acagtaagga tcttaactga taaactatct ttctagccct aaggctttct 
     7861 acttttaaag ttcgtttctc tttctctctc tctctctctc tctctctctc tctctccctc 
     7921 tccctccctc ctcctcctcc tcctctttcc cccctctcgc ctcctctttc tctctcccct 
     7981 ccctctctct ctctcacaca cacacacaga cagagagagg agagagaaag gaagccttac 
     8041 taattaatta cctagtttta gacataacta gtgggaatcc atagtaagta aatcaacatt 
     8101 cttgaagaga ggttagaaga attggctctc gttgtccatc gaggctgtat aagattattc 
     8161 ttctgttttc tcagcttacc atacatattt tctacgtaga caataccaat tcaaatacac 
     8221 tgtacgtaaa gtatatgcgc acacacacac acacacacac acacacacac acacaagtta 
     8281 catattgtca tatagtacta aaatttatgg actttttcat attaatacat ctagagctat 
     8341 catgtttttt atcttttaaa aattacttta cggggctaca gagatgtctc agcagttagg 
     8401 agcactgact gctcttccag aggtcctgag ttcaattccc agcaaccaca tggtggctca 
     8461 caaccatctg taatgggatc tgatccactc ttatggtgtg tctgaagaca gtgacagtgt 
     8521 actcatatac atgaaataaa tctttaaaaa attactttac catcagggta tatatatata 
     8581 tatatatgtg tgtgtgtgtg tgtgtgtgta tgaagtattt gctatttgag agggtctcac 
     8641 tgtttagctc agtctggctt ggaacgttct gtatagccca gactggcctc aactcagcag 
     8701 tccccctgaa aggctctcat atgtggagat tacaggtatg tgctactgtg cccagcccta 
     8761 catgttactt tgtttttgaa atagggtatc aaatagctta gactggcttc taagtcagtg 
     8821 tgtagctaag gatggtctga gcttctgaac ctgctacttc aactcctggt gtgccatcat 
     8881 ggccagtttg cacagttgtg agagtagaac tcaggacctc ctatgtgtta ctagacatgt 
     8941 tctctaccag ctgagctaca tcccaattcg cctacattta cttttatttt ttaaaagatt 
     9001 tatttatgta tacagcactc tgcctgcatg tacatctgca tgccagaaga gggcaccaga 
     9061 tctcatcata catagttgtg agccaccatg tggttgctgg ggattgaact cagggcctct 
     9121 ggaagagcag tcagtgctct taacctctga gccatctctg tagcccccta catttatttt 
     9181 aaagattata ggctattcta ttcatagatg tgccataact gatttaacca gatcttcatt 
     9241 attttgtact tagtagagta tattagaggg aatgaatctt aaatacacat tttgacactt 
     9301 gctcagcttt agacaagtaa aaatcactgg tcaaggaaat ggtgcttagt ggatgcttct 
     9361 gaactgtcaa attgtcctcc aacaagttac atcattttat acttccaacg ttatgtgaga 
     9421 gttcattttt ctttaggctc ttctagtatt ttatatttga gaaataaaat ttaaccaaaa 
     9481 tttgccaatc tagtaagctt gtttttattt gtatctttaa attttatgat agatgttata 
     9541 gtgtactctc ctctcccctc ccctcccctc tcctcctcag caggatcttg ttatgtagcc 
     9601 ctaagggatc taatacttac tctgtagtcc aggctgtcct tcagctcatg agacacacca 
     9661 tcatgcttgg catgtttctt gttctgaaag gtatctggtt ttaattttgg ttgagctggt 
     9721 gtgttggtgt atatctgtaa tctcagcaca ggcaagtaga gacaaggatt atgattacag 
     9781 tccagcctgg gctatttaga gggattctct ttaatctttc ctcatgcccc aaaatcttac 
     9841 ctttggtata tgaaaaatag tctccacttc tgcaaaatat tgctttatga agtaagaatt 
     9901 aattcccttc atagagacaa ggaatgtgtc ctgtaaaagt ttaatgtgta agaagtattt 
     9961 gttataaaag ataaatattc agaatcttct ttttaattcc tgattttatt tctataggac 
    10021 tgaaagactt gctcgagatg tcatgaagga gatgggaggc catcacattg tggccctctg 
    10081 tgtgctcaag gggggctata agttctttgc tgacctgctg gattacatta aagcactgaa 
    10141 tagaaatagt gatagatcca ttcctatgac tgtagatttt atcagactga agagctactg 
    10201 tgtaagtata attaacttat aattaaaaaa atagggccat tctagtttta tctatatttt 
    10261 ttttaaactt gtgcaaacta tgctacataa tcaacttact tgattatgta atatatatag 
    10321 taatagttgg aattactatt attacatatt tatttttctg tgctggggta gaacccaggg 
    10381 aactaagtac tgtaataacc gagcttcatt catcctagtc atttttaatt tttttttttt 
    10441 tttttgtttt ttcgagacaa ggtttctctg tgtagccctg gctgtcctgg aactcactct 
    10501 gtagaccagg ctggcctcga actcagaaat ctgcctgcct ctgcctccca gtgctgagat 
    10561 taatgacgtg caccaccact gcctggcatt tttttaaagt tttaatttga gaccctttct 
    10621 cactaacttg ctcacactat cctcgaactc attacgtggg ctggtctaga aatttcagtc 
    10681 aacttgcctc agcttctcaa gtagctggga ttacaggcag gtgctaccac ttctggccat 
    10741 gatcattgtt gttgttgcta ttattattat tattatttca ctgatacttc atatcacaac 
    10801 acagaatagt aactgtagtt catgaccaac aaaaaagttg tacagtttct ctgttgtctt 
    10861 attttgatct ggattttgat ttgtttcatg gcactgggca ggctaatctc aaactgcatg 
    10921 gctcaaatag tccttctgtc ttaatctccc atgttctgga agtataggta tcactacacc 
    10981 tagctgtatt ttcatatttt aaaaaaaacg cctaagaatt gctattgaat gaggcggaga 
    11041 gatggctcag cagtcacaag tacttgtaga ggactggggt ttggttacta gcacctcaaa 
    11101 gatggcttac aattatctgt agctccaatt ccagaggatc tgtgtggtaa agctgcatgt 
    11161 atatgggcat gcaggtaaaa ctgctaaaca tataaaaata gctcagtctt aagagaattg 
    11221 gtcgacgtaa gctcattcga gtcgactttt tcgccctttg acgttggagt ccacgttctt 
    11281 taatagtgdg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 
    11341 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 
    11401 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 
    11461 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 
    11521 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 
    11581 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
    11641 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 
    11701 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 
    11761 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 
    11821 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 
    11881 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
    11941 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 
    12001 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 
    12061 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 
    12121 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 
    12181 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
    12241 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 
    12301 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 
    12361 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 
    12421 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 
    12481 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
    12541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
    12601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
    12661 gctaccaggt cgagtaggga taacagggta atggatcctc gttgggcacc tgcactcatg 
    12721 tacacaggcc cacatataga tgtacacacg catatcattc aaaatagaaa ttatttatac 
    12781 aagtagtgcg ttggagagct ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac 
    12841 ccaagttcaa tttccagcac ctgtgttggg catctcacaa ccacatgtaa atctagtttc 
    12901 aggggatttc acccctcttc tgaactcttc aggcacttat acccacaaat ggacatctcc 
    12961 acatagagac acatactttt aaaaaagtaa tctagggtct agagaaatgg ttatagacca 
    13021 cggactggga tcagttccca gaagccacat cctgtggctc acaattgcct gtgaatcaag 
    13081 ttctagatct ggtggcctcg tctagcctgc agtcatcaac aaacacatgc acacatccat 
    13141 acacacataa tttaaaagaa agcgaaagta acctataatg ctaaacaaag cacattctca 
    13201 gtgtagactg cagtggtgat gaaccttgaa aatatattaa tagaagccag acacgtaagt 
    13261 cacaaatggt ataactcaat ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca 
    13321 aaaaggcaga ttattggttg tcagtagcta cagagatggg acattgaaga tattaatagg 
    13381 tatagggttg aggattcatg gcctttccct cttccatgtt agcatgtcta ttgttgttaa 
    13441 ccttattcaa ctcatgttag ggcaatcata ttgatgagac ttaatgagta tagcttgaca 
    13501 ttcctagaag acagagtctc acatcagact ctctgatcct ctgactctta gaatctttca 
    13561 acaccctctt ctgaaatgat ctctgagcct ttggttgtga ctgaattatg agaaagaatt 
    13621 ttatgttgaa tgaattttac ttcagcattt ttacaaaact gaagctgggg atggcgtttc 
    13681 atgcgtgcag tccctaccat ttgcctataa gcctggagga ggcagaagta gaattaggct 
    13741 tttcagggac atcttcagct gcatcatgag cttgaggaca acttgagcta cataagattc 
    13801 ccatccccac ccccacacac acacacacat ctagtatgta cctagtatgc agtcttttca 
    13861 tatgtttaaa atgtagaaag gtatatgtga agtctccctt ttccccacaa cttagtccct 
    13921 ccaaagtatt gcatcagttt ccttgtacat gattttatgc atatataaga tacactattt 
    13981 ctctctctct ctctctcctt ccctccttcc ctccaacctc tgggtatgag ttgagggtta 
    14041 tgtatgtttc aagttgtaat aagaattgcc actcaaacat ttcatcccat tttaagttaa 
    14101 gtttgtctga ttttccactt tagaagggca gccatgatgg ctcatgccta taatcccatc 
    14161 ctagcacttg taagcctaat acataagact ggaggattgt catcagcttg aggctagcct 
    14221 ggtctaccta atgagttcca gccagccaag agtagaatat aagaccctgt ctccaaaaaa 
    14281 tgtaccaaag gggctggagg ggtaactcag tggttaagaa cagtagctgc tcttccagag 
    14341 gacctgggtt caattcccag cacccacatg gcagcttgca actatctgta actgtccagc 
    14401 tttgggatat ccgacaccat tataccaatt acataaaatg aaaaaattgt aaaatgtgct 
    14461 aaaatgagtg gggaggggga gcatggtgtg gttctgtggg cttgggattt tgccatgaat 
    14521 ccatgactat atctcagcaa tatgtggatt atttctggaa acctgtgggg catgggtgta 
    14581 tgggaagagg ggagagagag agagcccgca tccagccaga gttcctgtgc tctgggcagg 
    14641 cggacgtggg aggactgctg gacgatttcc acttggccct gggtgggcat ctggctgtgt 
    14701 gaagccactg accccactca gcggggggta gataaggggc atttcccata ccaggttctc 
    14761 agactccagc attccacgct ggatacagca gaagagctga gaacagaata ggggccccag 
    14821 ggagacactc tcgtcccaga gataaagaag gcagagggag agagattcct acgcaggcga 
    14881 gaggccttag tctgggcctt gatgaacaga gacggtctat ggttttagag ctttattgta 
    14941 gaaaggcagg gggaaagaga gaaggtagaa agagagagac cagccatggc caagaggaga 
    15001 gaagggggga aaggagagag agaagaaagg ctagagagta agaaaggtta aagagacaga 
    15061 agtaaaagct tagagtaaga gagagagaga gagcaagagg gagagtaaga aaagtaaaag 
    15121 cttaagagag cgaggagggg ccagcccctc taatagtggg cttgttatct tgttgttgct 
    15181 aggtaactgt gaagcgtcta gcctgaaggc cagaagcttg ggacattgtc tatgtgactg 
    15241 aaagccatga gcctctactg tgggggctgt ggaggtggta acttggacag gagccagggg 
    15301 ttccaggaga catgagggaa tgccttctgt cccatgtagg taggaattac caccaccggc 
    15361 tttcaccgta gttcactgga gactgtttgt acatagccca ttgccccaca gaaaccaggc 
    15421 actcactgtt tcatttgcat aagaattaga aggctttatt ttggctggga ttggtgactt 
    15481 gccccagaaa ttccagcttt tgggaagccg aatcctggag aaccaggagt tcaaggtcat 
    15541 cctcaggtac ctcatttgtt caagaccagc ctgtgctacc atgagacctt gtccctactc 
    15601 ccctcaaccc caaaacacgc tgttttcatt ttataggttg tactaaccat ggaggatggt 
    15661 catttgcctt tctcttgatt tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt 
    15721 tccagatagg cttgcctctc cacagccagg cttatcctgc ttcagtccca tgtttggcaa 
    15781 gggcaacata tgactgaaga acttgcatag ccttatggtg tatcatgact cttagcccca 
    15841 gtttataact gtctaacagc tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt 
    15901 taacttttca gctgtttgtt ttgactttgc ataaatactg agactagcta ggaaatgagc 
    15961 ttttgtttca tttgctaaaa atgatttttt tttcatgagg caatttcaag caatttgaat 
    16021 ttagcaatct ctacttgtct gaaaagaagt tattaattaa aaaaaaacaa acaaacagga 
    16081 acctattatg ctggctagtc acatgaaaga atccaaatga acacagtcaa ggctgaatca 
    16141 acacatgaaa ttattttcta attccccaaa ttacatttat gctaattagt atgtagtaaa 
    16201 acattttaat tgtttccaat gttaaacatt acagaaataa ttttgatgtt ttcctaataa 
    16261 cccagccttt gatcatgttt gcatttattt gattgttgtc agtttattaa ataattaagt 
    16321 aagagcaaca agataaaatg acttcatgag agtccttttc tgtaacttag aacgtcagta 
    16381 gtcataggaa ctgcggtcgt tcccttacct ttcagaagta ggctaaagag ttgaacgcaa 
    16441 aggtggtttt tcgaatcttc ctgatcctta agcccccact aggtttatat tctatatttg 
    16501 atatcggatt cgaacccctc gagcttagga tccggaaccc ttaatataac taagttatta 
    16561 ggtccctcga agaggttcac tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc 
    16621 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 
    16681 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 
    16741 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 
    16801 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 
    16861 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 
    16921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
    16981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
    17041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
    17101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
    17161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
    17221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
    17281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
    17341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
    17401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
    17461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
    17521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
    17581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
    17641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
    17701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
    17761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
    17821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
    17881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
    17941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
    18001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
    18061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
    18121 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt 
    18181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
    18241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
    18301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
    18361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
    18421 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 
    18481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
    18541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
    18601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
    18661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
    18721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
    18781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tg 
//
