LOCUS       Ple64\(pEMS1129)       18357 bp    DNA     circular     10-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|545594197|
COMMENT     VNTDBDATE|545594197|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple64 (pEMS1129)|
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COMMENT        (mapper: 23.4882 -16.7196 40.0378 40.0378 0.01 10 -1 18357 18357 1 0 0) 
COMMENT        #138=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1937009781 100) 
COMMENT              (CObjectList 
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COMMENT                     (CObjectList 
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COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27453 6.27253 1 0.0214037) 0.0341733) 
COMMENT                      #141=(CLabel 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple64 (pEMS1129)" "@N" 1 0 
COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
COMMENT                      #142=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #100# 1094797127 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "18357 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 3.19698 0.949103 Nil)) (CObjectList)) 
COMMENT               #143=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -953 100) 
COMMENT                     (CObjectList 
COMMENT                      #144=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -534 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.91163 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5 mutant)" "@N" 1 9.61021 12.6519 0 
COMMENT                                   -10 8.21723 1.17389 #145#) 
COMMENT                             #147=(CScratch 
COMMENT                                   (CWidget 0 (3 #1# 0) 1 2 0 0 #107# 1221 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.90001 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus sequence" "@N" 1 12.4313 
COMMENT                                   10.9845 0 -10 12.7629 1.17389 #147#) 
COMMENT                             #149=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   7.68927 9.44198 0 -10 2.67247 1.17389 #149#) 
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COMMENT                                     2081903016 100) (LOGPEN 0 0 6723840) 1 
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COMMENT                             #152=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Cre" "@N" 1 
COMMENT                                   10.9503 8.52387 0 -10 1.6984 1.17389 #151#) 
COMMENT                             #153=(CScratch 
COMMENT                                   (CWidget 0 (3 #4# 0) 1 2 0 0 #107# 4063688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.30027 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #154=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #99# 840986223 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   14.1257 4.35883 0 -10 1.99811 1.17389 #153#) 
COMMENT                             #155=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.2104 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #156=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #99# 21294100 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.8281 0 0 
COMMENT                                   -10 11.0645 1.17389 #155#) 
COMMENT                             #157=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #103# 
COMMENT                                     2081903016 100) (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.84005 
COMMENT                                    5.19126 1 0.082322) 0.8 1.8 0) 
COMMENT                             #158=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.381 
COMMENT                                   -2.96617 0 -10 14.9609 1.17389 #157#) 
COMMENT                             #159=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #103# 
COMMENT                                     2081903016 100) (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.59409 3.84005 1 
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COMMENT                             #160=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 6.25132 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #159#) 
COMMENT                             #161=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #103# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.79487 2.09755 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #162=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.4817 
COMMENT                                   2.46665 0 -10 10.4901 1.17389 #161#) 
COMMENT                             #163=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.10405 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -12.2572 -6.62567 0 -10 3.02214 1.17389 #163#) 
COMMENT                             #165=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #103# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.91437 
COMMENT                                    6.22124 1 0.082322) 0.8 1.8 0) 
COMMENT                             #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 16 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Ple64 (FEV-A)" 
COMMENT                                   "@N" 1 6.80677 14.3882 0 -10 6.31903 1.17389 
COMMENT                                   #165#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                      #169=(CGroupWidget 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   15.4496 2.76636 0 -10 2.72243 1.17389 #170#) 
COMMENT                             #172=(CWideArrow 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
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COMMENT                             #174=(CWideArrow 
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COMMENT               #176=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -813 100) 
COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #178=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1092 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
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FEATURES             Location/Qualifiers
     misc_feature    1059..1092
                     /vntifkey="21"
                     /label=MFRT\(F5\mutant)
     misc_feature    1093..1104
                     /vntifkey="21"
                     /label=Kozak\consensus\sequence
     misc_feature    1105..1821
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    1822..2847
                     /vntifkey="21"
                     /label=Cre
     misc_feature    2848..2920
                     /vntifkey="21"
                     /label=NLS
     misc_marker     2924..2957
                     /vntifkey="22"
                     /label=MFRT
     misc_feature    3111..3161
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    3167..7120
                     /vntifkey="21"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    7121..10766
                     /vntifkey="21"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_feature    12220..16031
                     /vntifkey="21"
                     /label=5'\homology\sequence
     misc_marker     10768..12194
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     complement(17351..18351)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    12201..12218
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    153..1050
                     /vntifkey="21"
                     /label=Ple64\(FEV-A)
                     /note="1302"
     misc_feature    153..1050
                     /vntifkey="21"
                     /label=Region\Prom
BASE COUNT     4853 a      4149 c      4322 g      5033 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccac gcgtggcgcg ccaggaggta aagagagacc tagaaggggg 
      181 cggggaggac agaagagggg gccacccatc ataagttgcc aggagagatg gcccagcacc 
      241 aggcctgagg cttgggggcc ccagaaaggc aggaaacggg ccaaagcaag aaggagacgg 
      301 ggaaggggtt cgggtctcta gggaagagca gagagtggga gaagaggagg agggagcggg 
      361 gatgcgggca gagataaagg gagcaacggc tgcagggccg ggcgcgcagc gacaggcgag 
      421 agggagggaa gcggaaattt aaaagtgaag atgcagataa cgcagcctgg agacgggaac 
      481 ccgggtgggg atagagggag ggagggggag gagagtgagc ggcgagtgga gcgggagccg 
      541 actggaagaa gggctctagg gagggggctg tggctgctgg ggtccgaggt ggggccgggt 
      601 acaccagccc catcactgtt tgcagagagt cagggaggcg gaaaagacac gcgctctagg 
      661 ctcccatcag ggcacatggc ccgggcccat cccccgcgcg tctccccggc tgcggggcgc 
      721 ggggggctgc cgggtgcgct tggctgtggc gcggcgcgtt ggagacttta ttgcgatggg 
      781 acgataagag gggcgggggc ggggtcctgg gggccgaggc ggcagcgctt taattaaaac 
      841 ggaaattgcg gccccgggcc gcgcgggggc cggagggttc caagcggccc cttagctgga 
      901 agcgtttctc caggaccccc ccgcaacccc cgccacgccc gggctgcccc ctcccgccag 
      961 gccctgccgg acccggcgcc gtcttctcct ccttgtcacc cgcggtcgct tcgggcgggg 
     1021 atcggtgcca ccgagcgcaa agcctgcctc ggcgcgccga agttcctatt cttcaaaagg 
     1081 tataggaact tcgccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg 
     1141 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 
     1201 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 
     1261 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 
     1321 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 
     1381 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 
     1441 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 
     1501 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 
     1561 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 
     1621 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 
     1681 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 
     1741 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 
     1801 atggacgagc tgtacaagtt atccaattta ctgaccgtac accaaaattt gcctgcatta 
     1861 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc 
     1921 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca 
     1981 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat 
     2041 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag 
     2101 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca 
     2161 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct 
     2221 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc 
     2281 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata 
     2341 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta 
     2401 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc 
     2461 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg 
     2521 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc 
     2581 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc 
     2641 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga 
     2701 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc 
     2761 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca 
     2821 atggtgcgcc tgctggaaga tggcgatgat ccaaaaaaga agagaaaggt agatccaaaa 
     2881 aagaagagaa aggtagatcc aaaaaagaag agaaaggtaa taagaagttc ctattctcta 
     2941 gaaagtatag gaacttcagc ggccgcgact ctagatcata atcagccata ccacatttgt 
     3001 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 
     3061 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 
     3121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cgaattctcg taaaactctt 
     3181 catggcagta gttattattc tctctctctc tctttttctt ttttcttgag acaggatatt 
     3241 tctctgttgc ccaggctgga gtgcagtggc acagtcttgg ctcactgcag cctggacctc 
     3301 ctgggctcaa gccatcctcc cacctcagcc tcccaagtag ctggggctac aggcacatgg 
     3361 ccaccaggcc cagataattt ttcatttttg tagagactga gtctcaccat gttacccagg 
     3421 tttattattc tcatttttta gatgaagaga ctgaggtcca gagaagctca atgacttgcc 
     3481 tagttttaca aatctcctgc catcacatac ccctcagcgt ccttaataag agggaggcca 
     3541 ccaactatgt gctgggcact gtggtggatg ctggagctat agggttgagt atataagaaa 
     3601 tggtgttgct ggagcaactg ttgcttgctt acctgaccta tctgagaatt aattagcagg 
     3661 ggaacatatt tttgttttca gattcaatat aagaacttgt gtgggcaaaa ataaagatca 
     3721 gtagtaataa cagtagttcc catttgctga ctgtactgtc ctaagtgcat atatatatac 
     3781 atacacacac gcatacctat actcctctaa tactcaaaat gatcctgttt atgtattgtt 
     3841 aatatgctca ttttattttt aaatttttat ttatttttat ttttatttat ttttgagacg 
     3901 gagtctcatt ctgtcgcgga ggctgaagtg cagtggtgcg atctcagctc agtgcgacct 
     3961 ccgcctcccg ggttcaagtg attctcctgc ctcagcctcc ggactagctg ggattacagg 
     4021 cgcccgcctc cacgcccagc taatttttgt atttttagta gagatggggt ttcgccatgt 
     4081 tggccaggct ggtctcgtac tcctgacctt gagtgatcca cctgcctcgg cctcccaaag 
     4141 tgctgggatt acaggcatga gccaccgcgc cgggctaata tgctcatttt agtgaggcaa 
     4201 aaatagaggc tcagagtctg atttgtacaa aactacagag cagttaagtg tcctctcaga 
     4261 tgtgtaccct gatctgggtg actctaggac tctaggtctc aactgttaca accagttaag 
     4321 ggtttgggga agcactgggc caagagtcag gaaaatggaa gccacaggta gtgcaaggtc 
     4381 ttgggaatgg gacgtctggt ccaaggattc acgcgatgac tggaacccga agagccgggg 
     4441 cccggtttac ggccgccatg aagcaacgcg cgccggtagg tttgggaatc agggagccct 
     4501 ctgaatagga gactgagttg ggagggaaag gggcttcgct gggggagcct cggcttcttc 
     4561 tgggagaaaa ttcccacggc tacctagtga gcctgcaaac tggtaggcgc cggcgtaggc 
     4621 gcgcgggcgg ggccgggggc ggggcctgcg gggcgtggcg gggcgggcag agggcggggc 
     4681 ctgcttctcc tcagcttcag gcggctgcga cgagccctca ggcgaacctc tcggctttcc 
     4741 cgcgcggcgc cgcctcttgc tgcgcctccg cctcctcctc tgctccgcca ccggcttcct 
     4801 cctcctgagc agtcagcccg cgcgccggcc ggctccgtta tggcgacccg cagccctggc 
     4861 gtcgtggtga gcagctcggc ctgccggccc tggccggttc aggcccacgc ggcaggtggc 
     4921 ggccgggccc tgaggcgcgg gatcgatcca tgacaccccc ccgcccccca tatctcacta 
     4981 taattcactg tcaaatggct tttatgattt ggagtttggg gatactttta aagacagggt 
     5041 cttggtagct cctagatggc ttgggattca cttagtaacc cttgctggcc taaaattcag 
     5101 gaacttctac ctcatcttcc caagtactgg gttagtaggg atgtccagca tatttagctt 
     5161 gctttaatga ttttgctgta cttttttctg tgtgtacagg tgcatgtata tttataaaac 
     5221 taacataata tattttaccc ccaaagtacc ataaccctaa ttaatgctat taaatgtttt 
     5281 gtatgctggg ctctgggcat actaatataa ccaatattat acaatgcaaa acaattttgg 
     5341 aatatcttct gtaaaattat cttattagta agttgtaacc atctcagact tctaagtttg 
     5401 agtttgtaaa ggatcagttt tttttggtga cctacacaca cacacacaca cacacacaca 
     5461 cacacacaca caggggggtg gattctgaga atttagagaa tttagatggt taataccagt 
     5521 ttttattttt attctgtgtg atatttattt attaagattt atttatttat tacatgtagg 
     5581 tacactgtat ctgtcttcag acacaccaga agagggcatc agatctcgtt acagatggtt 
     5641 gtgagtcacc atgtggttgc tgggatttga actcaggacc tctggaagaa cagtcagtgc 
     5701 tcttaaccgt tgagccatct caccagcccc gtgatatttt atttaactta ctagaatata 
     5761 ctgtgttaaa acatatttta ggggattggg gagatggccc attctgtaaa gtgtctgcca 
     5821 tgtcatcttc aggacctgcg tttgtaaccc cagcatggag aagacagaga cagggagacc 
     5881 cctgggcttc ctggcaagct agactagcaa atttggttaa ctaggttcaa tgagagactc 
     5941 agtctcaaaa cataagaagt gaatttttta gacccaattt caatctctga actccatacg 
     6001 tatgcacatg tacctgcata taaatatata tacacaccca taaacacatg cacacacaaa 
     6061 aaatggttca aaagttctta aaatgtataa gagtagaaag tgtagagccg ggagtggtgg 
     6121 cgcacgcctt taatcccagc acttgggagc agaggcaggt ggatttctga gttcgaggcc 
     6181 agcctggtct acagagtgag ttccaggaca gccagagcta tacagagaaa tcctgcctca 
     6241 aaaaaccaaa aataaagtgt atatactaat ctttcatgcc taaaatattt atttttgcca 
     6301 catttttcat acctctttaa tatttttctt tttctctttg tgcttcaaag gaaattttgt 
     6361 gtcatgtttt acttttcatt cttgactcta ttcagcagta agacgcagca tggctttctt 
     6421 agagaagtat aatctattac aaaacggtaa catttaactg ctctacatac cctcttgtag 
     6481 ctaaattcct ctgttaaact aagacttatt atctaaacgt aattaaatgt tgatatctta 
     6541 taatgctcca ctttgaaaca gctgtctttc ttttgcagat tagcgatgat gaaccaggtt 
     6601 atgacctaga tttgttttgt atacctaatc attatgccga ggatttggaa aaagtgttta 
     6661 ttcctcatgg actgattatg gacaggttag taagacctcg attgaagtta tttttagagg 
     6721 gtgtgtgtgt ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taagagagag 
     6781 aggagaggca tgtgtgatat atatgtgaag tgtattgcat gtacatggaa tccagagaag 
     6841 gacattaggt gtcctactct gttgctctct tattcccttg aatcagtgtc tcttgctgaa 
     6901 cctggggcat acatactgat ttggctaggc tgcctggcta gcaagaccca gggatctgct 
     6961 tgtctctgcc ccccagtgct gacgttacaa gcaggtgcaa ccacatctgg tttttatatg 
     7021 ggtactgggg gatctgaact tagggcctga tgcgtgccca agtgctctca cccgccaagc 
     7081 cacctctcca gccaaagggt tttttttctt gtattaattc taacttagca actgttgagt 
     7141 agttgatgtt tgaatataag ctaagccaac ttattagaat aaatgttttc tattttttac 
     7201 ttgtatgtgt gagtgcctgc atgtatgtat gtgaagcata tgtattccag gatgtcagaa 
     7261 ttgtgatgga ttccctggaa ctggagttac agaccattgt gaaccactgt gtatgggtgc 
     7321 tgggaactga actttaggtc ctctgcaaaa acagtaagga tcttaactga taaactatct 
     7381 ttctagccct aaggctttct acttttaaag ttcgtttctc tttctctctc tctctctctc 
     7441 tctctctctc tctctccctc tccctccctc ctcctcctcc tcctctttcc cccctctcgc 
     7501 ctcctctttc tctctcccct ccctctctct ctctcacaca cacacacaga cagagagagg 
     7561 agagagaaag gaagccttac taattaatta cctagtttta gacataacta gtgggaatcc 
     7621 atagtaagta aatcaacatt cttgaagaga ggttagaaga attggctctc gttgtccatc 
     7681 gaggctgtat aagattattc ttctgttttc tcagcttacc atacatattt tctacgtaga 
     7741 caataccaat tcaaatacac tgtacgtaaa gtatatgcgc acacacacac acacacacac 
     7801 acacacacac acacaagtta catattgtca tatagtacta aaatttatgg actttttcat 
     7861 attaatacat ctagagctat catgtttttt atcttttaaa aattacttta cggggctaca 
     7921 gagatgtctc agcagttagg agcactgact gctcttccag aggtcctgag ttcaattccc 
     7981 agcaaccaca tggtggctca caaccatctg taatgggatc tgatccactc ttatggtgtg 
     8041 tctgaagaca gtgacagtgt actcatatac atgaaataaa tctttaaaaa attactttac 
     8101 catcagggta tatatatata tatatatgtg tgtgtgtgtg tgtgtgtgta tgaagtattt 
     8161 gctatttgag agggtctcac tgtttagctc agtctggctt ggaacgttct gtatagccca 
     8221 gactggcctc aactcagcag tccccctgaa aggctctcat atgtggagat tacaggtatg 
     8281 tgctactgtg cccagcccta catgttactt tgtttttgaa atagggtatc aaatagctta 
     8341 gactggcttc taagtcagtg tgtagctaag gatggtctga gcttctgaac ctgctacttc 
     8401 aactcctggt gtgccatcat ggccagtttg cacagttgtg agagtagaac tcaggacctc 
     8461 ctatgtgtta ctagacatgt tctctaccag ctgagctaca tcccaattcg cctacattta 
     8521 cttttatttt ttaaaagatt tatttatgta tacagcactc tgcctgcatg tacatctgca 
     8581 tgccagaaga gggcaccaga tctcatcata catagttgtg agccaccatg tggttgctgg 
     8641 ggattgaact cagggcctct ggaagagcag tcagtgctct taacctctga gccatctctg 
     8701 tagcccccta catttatttt aaagattata ggctattcta ttcatagatg tgccataact 
     8761 gatttaacca gatcttcatt attttgtact tagtagagta tattagaggg aatgaatctt 
     8821 aaatacacat tttgacactt gctcagcttt agacaagtaa aaatcactgg tcaaggaaat 
     8881 ggtgcttagt ggatgcttct gaactgtcaa attgtcctcc aacaagttac atcattttat 
     8941 acttccaacg ttatgtgaga gttcattttt ctttaggctc ttctagtatt ttatatttga 
     9001 gaaataaaat ttaaccaaaa tttgccaatc tagtaagctt gtttttattt gtatctttaa 
     9061 attttatgat agatgttata gtgtactctc ctctcccctc ccctcccctc tcctcctcag 
     9121 caggatcttg ttatgtagcc ctaagggatc taatacttac tctgtagtcc aggctgtcct 
     9181 tcagctcatg agacacacca tcatgcttgg catgtttctt gttctgaaag gtatctggtt 
     9241 ttaattttgg ttgagctggt gtgttggtgt atatctgtaa tctcagcaca ggcaagtaga 
     9301 gacaaggatt atgattacag tccagcctgg gctatttaga gggattctct ttaatctttc 
     9361 ctcatgcccc aaaatcttac ctttggtata tgaaaaatag tctccacttc tgcaaaatat 
     9421 tgctttatga agtaagaatt aattcccttc atagagacaa ggaatgtgtc ctgtaaaagt 
     9481 ttaatgtgta agaagtattt gttataaaag ataaatattc agaatcttct ttttaattcc 
     9541 tgattttatt tctataggac tgaaagactt gctcgagatg tcatgaagga gatgggaggc 
     9601 catcacattg tggccctctg tgtgctcaag gggggctata agttctttgc tgacctgctg 
     9661 gattacatta aagcactgaa tagaaatagt gatagatcca ttcctatgac tgtagatttt 
     9721 atcagactga agagctactg tgtaagtata attaacttat aattaaaaaa atagggccat 
     9781 tctagtttta tctatatttt ttttaaactt gtgcaaacta tgctacataa tcaacttact 
     9841 tgattatgta atatatatag taatagttgg aattactatt attacatatt tatttttctg 
     9901 tgctggggta gaacccaggg aactaagtac tgtaataacc gagcttcatt catcctagtc 
     9961 atttttaatt tttttttttt tttttgtttt ttcgagacaa ggtttctctg tgtagccctg 
    10021 gctgtcctgg aactcactct gtagaccagg ctggcctcga actcagaaat ctgcctgcct 
    10081 ctgcctccca gtgctgagat taatgacgtg caccaccact gcctggcatt tttttaaagt 
    10141 tttaatttga gaccctttct cactaacttg ctcacactat cctcgaactc attacgtggg 
    10201 ctggtctaga aatttcagtc aacttgcctc agcttctcaa gtagctggga ttacaggcag 
    10261 gtgctaccac ttctggccat gatcattgtt gttgttgcta ttattattat tattatttca 
    10321 ctgatacttc atatcacaac acagaatagt aactgtagtt catgaccaac aaaaaagttg 
    10381 tacagtttct ctgttgtctt attttgatct ggattttgat ttgtttcatg gcactgggca 
    10441 ggctaatctc aaactgcatg gctcaaatag tccttctgtc ttaatctccc atgttctgga 
    10501 agtataggta tcactacacc tagctgtatt ttcatatttt aaaaaaaacg cctaagaatt 
    10561 gctattgaat gaggcggaga gatggctcag cagtcacaag tacttgtaga ggactggggt 
    10621 ttggttacta gcacctcaaa gatggcttac aattatctgt agctccaatt ccagaggatc 
    10681 tgtgtggtaa agctgcatgt atatgggcat gcaggtaaaa ctgctaaaca tataaaaata 
    10741 gctcagtctt aagagaattg gtcgactgtc gactttttcg ccctttgacg ttggagtcca 
    10801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
    10861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
    10921 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 
    10981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 
    11041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
    11101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 
    11161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 
    11221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 
    11281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 
    11341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 
    11401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 
    11461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 
    11521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 
    11581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 
    11641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 
    11701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 
    11761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 
    11821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 
    11881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 
    11941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 
    12001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
    12061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
    12121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
    12181 accaccgcta ccaggtcgag tagggataac agggtaatgg atcctcgttg ggcacctgca 
    12241 ctcatgtacg caggcccaca tatagatgta cacacgcata tcattcaaaa tagaaattat 
    12301 ttatacaagt agtgcgttgg agagctggct caggagttgt taatgcttgc tacccttgca 
    12361 aaggacccaa gttcaatttc cagcacctgt gttgggcatc tcacaaccac atgtaaatct 
    12421 agtttcaggg gatttcaccc ctcttctgaa ctcttcaggc acttataccc acaaatggac 
    12481 atctccacat agagacacat acttttaaaa aagtaatcta gggtctagag aaatggttat 
    12541 agaccacgga ctgggatcag ttcccagaag ccacatcctg tggctcacaa ttgcctgtga 
    12601 atcaagttct agatctggtg gcctcgtcta gcctgcagtc atcaacaaac acatgcacac 
    12661 atccatacac acataattta aaagaaagcg aaagtaacct ataatgctaa acaaagcaca 
    12721 ttctcagtgt agactgcagt ggtgatgaac cttgaaaata tattaataga agccagacac 
    12781 gtaagtcaca aatggtataa ctcaatttat atgaaattcc cagagagtcc aaagttaaaa 
    12841 aaaacaaaaa ggcagattat tggttgtcag tagctacaga gatgggacat tgaagatatt 
    12901 aataggtata gggttgagga ttcatggcct ttccctcttc catgttagca tgtctattgt 
    12961 tgttaacctt attcaactca tgttagggca atcatattga tgagacttaa tgagtatagc 
    13021 ttgacattcc tagaagacag agtctcacat cagactctct gatcctctga ctcttagaat 
    13081 ctttcaacac cctcttctga aatgatctct gagcctttgg ttgtgactga attatgagaa 
    13141 agaattttat gttgaatgaa ttttacttca gcatttttac aaaactgaag ctggggatgg 
    13201 cgtttcatgc gtgcagtccc taccatttgc ctataagcct ggaggaggca gaagtagaat 
    13261 taggcttttc agggacatct tcagctgcat catgagcttg aggacaactt gagctacata 
    13321 agattcccat ccccaccccc acacacacac acacatctag tatgtaccta gtatgcagtc 
    13381 ttttcatatg tttaaaatgt agaaaggtat atgtgaagtc tcccttttcc ccacaactta 
    13441 gtccctccaa agtattgcat cagtttcctt gtacatgatt ttatgcatat ataagataca 
    13501 ctatttctct ctctctctct ctccttccct ccttccctcc aacctctggg tatgagttga 
    13561 gggttatgta tgtttcaagt tgtaataaga attgccactc aaacatttca tcccatttta 
    13621 agttaagttt gtctgatttt ccactttaga agggcagcca tgatggctca tgcctataat 
    13681 cccatcctag cacttgtaag cctaatacat aagactggag gattgtcatc agcttgaggc 
    13741 tagcctggtc tacctaatga gttccagcca gccaagagta gaatataaga ccctgtctcc 
    13801 aaaaaatgta ccaaaggggc tggaggggta actcagtggt taagaacagt agctgctctt 
    13861 ccagaggacc tgggttcaat tcccagcacc cacatggcag cttgcaacta tctgtaactg 
    13921 tccagctttg ggatatccga caccattata ccaattacat aaaatgaaaa aattgtaaaa 
    13981 tgtgctaaaa tgagtgggga gggggagcat ggtgtggttc tgtgggcttg ggattttgcc 
    14041 atgaatccat gactatatct cagcaatatg tggattattt ctggaaacct gtggggcatg 
    14101 ggtgtatggg aagaggggag agagagagag cccgcatcca gccagagttc ctgtgctctg 
    14161 ggcaggcgga cgtgggagga ctgctggacg atttccactt ggccctgggt gggcatctgg 
    14221 ctgtgtgaag ccactgaccc cactcagcgg ggggtagata aggggcattt cccataccag 
    14281 gttctcagac tccagcattc cacgctggat acagcagaag agctgagaac agaatagggg 
    14341 ccccagggag acactctcgt cccagagata aagaaggcag agggagagag attcctacgc 
    14401 aggcgagagg ccttagtctg ggccttgatg aacagagacg gtctatggtt ttagagcttt 
    14461 attgtagaaa ggcaggggga aagagagaag gtagaaagag agagaccagc catggccaag 
    14521 aggagagaag gggggaaagg agagagagaa gaaaggctag agagtaagaa aggttaaaga 
    14581 gacagaagta aaagcttaga gtaagagaga gagagagagc aagagggaga gtaagaaaag 
    14641 taaaagctta agagagcgag gaggggccag cccctctaat agtgggcttg ttatcttgtt 
    14701 gttgctaggt aactgtgaag cgtctagcct gaaggccaga agcttgggac attgtctatg 
    14761 tgactgaaag ccatgagcct ctactgtggg ggctgtggag gtggtaactt ggacaggagc 
    14821 caggggttcc aggagacatg agggaatgcc ttctgtccca tgtaggtagg aattaccacc 
    14881 accggctttc accgtagttc actggagact gtttgtacat agcccattgc cccacagaaa 
    14941 ccaggcactc actgtttcat ttgcataaga attagaaggc tttattttgg ctgggattgg 
    15001 tgacttgccc cagaaattcc agcttttggg aagccgaatc ctggagaacc aggagttcaa 
    15061 ggtcatcctc aggtacctca tttgttcaag accagcctgt gctaccatga gaccttgtcc 
    15121 ctactcccct caaccccaaa acacgctgtt ttcattttat aggttgtact aaccatggag 
    15181 gatggtcatt tgcctttctc ttgatttcag aagcgtagcg cgggctttgc accatgatct 
    15241 agtgtttcca gataggcttg cctctccaca gccaggctta tcctgcttca gtcccatgtt 
    15301 tggcaagggc aacatatgac tgaagaactt gcatagcctt atggtgtatc atgactctta 
    15361 gccccagttt ataactgtct aacagctggt ggcttttgtg aaaaaggaaa gcatcatttg 
    15421 aacttttaac ttttcagctg tttgttttga ctttgcataa atactgagac tagctaggaa 
    15481 atgagctttt gtttcatttg ctaaaaatga tttttttttc atgaggcaat ttcaagcaat 
    15541 ttgaatttag caatctctac ttgtctgaaa agaagttatt aattaaaaaa aaacaaacaa 
    15601 acaggaacct attatgctgg ctagtcacat gaaagaatcc aaatgaacac agtcaaggct 
    15661 gaatcaacac atgaaattat tttctaattc cccaaattac atttatgcta attagtatgt 
    15721 agtaaaacat tttaattgtt tccaatgtta aacattacag aaataatttt gatgttttcc 
    15781 taataaccca gcctttgatc atgtttgcat ttatttgatt gttgtcagtt tattaaataa 
    15841 ttaagtaaga gcaacaagat aaaatgactt catgagagtc cttttctgta acttagaacg 
    15901 tcagtagtca taggaactgc ggtcgttccc ttacctttca gaagtaggct aaagagttga 
    15961 acgcaaaggt ggtttttcga atcttcctga tccttaagcc cccactaggt ttatattcta 
    16021 tatttgatat cggattcgaa cccctcgagc ttaggatccg gaacccttaa tataactaag 
    16081 ttattaggtc cctcgaagag gttcactagt actggccatt gcggccgctt cgaggctgcc 
    16141 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
    16201 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
    16261 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 
    16321 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 
    16381 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 
    16441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
    16501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
    16561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
    16621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
    16681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
    16741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
    16801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
    16861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
    16921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
    16981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
    17041 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
    17101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
    17161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
    17221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
    17281 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
    17341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
    17401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
    17461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
    17521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
    17581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
    17641 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 
    17701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
    17761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
    17821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
    17881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
    17941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 
    18001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
    18061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
    18121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
    18181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
    18241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
    18301 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgg 
//
