LOCUS       Ple79\(pEMS1433)       17693 bp    DNA     circular     15-JAN-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2316 - 5' ATGTGTCTTCCAACAGGTGAATACG -  3' Tm=56.0 Deg C
            Antisense primer: oEMS2308 - 5'  TGTTCCAATAGGCTCAGCGTTTAT - 3 Tm=56.1 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            327 bp product
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543239283|
COMMENT     VNTDBDATE|543239283|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.5632 -19.7668 40.0378 40.0378 0.01 10 -1 17693 17693 1 0 0) 
COMMENT        #149=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1023 100) 
COMMENT              (CObjectList 
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COMMENT                     (CObjectList 
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COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
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COMMENT               #154=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1232 100) 
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COMMENT                                   -11.4696 -6.96932 0 -10 3.02214 1.17389 #156#) 
COMMENT                             #158=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #118# 1040 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.78 1.9 0.082322 
COMMENT                                   1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   "MFRT (F5mutant)" "@N" 1 12.2822 8.71165 0 
COMMENT                                   -10 7.91752 1.17389 #158#) 
COMMENT                             #160=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 13.6554 6.96932 0 
COMMENT                                   -10 7.99245 1.17389 #160#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 15.9718 
COMMENT                                   3.48466 0 -10 10.0405 1.17389 #164#) 
COMMENT                             #166=(CScratch 
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COMMENT                                   12.7707 1.74233 0 -10 1.99811 1.17389 #166#) 
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COMMENT                             #170=(CScratch 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 16.7258 
COMMENT                                   -1.74233 0 -10 11.0645 1.17389 #170#) 
COMMENT                             #172=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.22816 1 0.082322) 0.8 1.8 0) 
COMMENT                             #173=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Ple79 (GABRA6-C)" "@N" 1 7.99421 13.9386 0 
COMMENT                                   -10 8.36709 1.17389 #172#) 
COMMENT                             #174=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Region 4" "@N" 
COMMENT                                   1 4.78037 17.4233 0 -10 4.19603 1.17389 #174#) 
COMMENT                             #176=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.0218 1 0.082322) 0.8 1.8 0) 
COMMENT                             #177=(CLabel 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Prom Region" 
COMMENT                                   "@N" 1 9.19154 10.454 0 -10 6.16917 1.17389 
COMMENT                                   #176#)) (CObjectList)) 
COMMENT                      #178=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -325 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                             #180=(CLabel 
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COMMENT                                   -4.68039 12.1963 0 -10 2.87229 1.17389 #179#) 
COMMENT                             #181=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.18514 2.69074 1 0.082322) 0.8 1.8 0) 
COMMENT                             #182=(CLabel 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -10.0172 -8.71165 0 -10 2.87229 1.17389 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1093 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #118# 652 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.05974 1.9 
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COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 21520428 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2316" "@N" 
COMMENT                                   1 5.7807 15.681 0 -10 4.79547 1.17389 #184#) 
COMMENT                             #186=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #118# 1021 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.95231 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2308" "@N" 
COMMENT                                   1 6.80828 12.1963 0 -10 4.79547 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -744 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    2.17627 1 0.082322) 0.8 1.8 0) 
COMMENT                             #190=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.5493 0 0 
COMMENT                                   -10 10.4901 1.17389 #189#) 
COMMENT                             #191=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.68896 3.98168 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #192=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 21586468 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 7.65384 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #191#) 
COMMENT                             #193=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.98168 
COMMENT                                    5.3836 1 0.082322) 0.8 1.8 0) 
COMMENT                             #194=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.5755 
COMMENT                                   -3.48466 0 -10 14.9609 1.17389 #193#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #195=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -256 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #196=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #197=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 966 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #198=(AnlzFontColorItem 602 626 33587200) 
COMMENT         #199=(AnlzFontColorItem 905 928 33587200)) 1 
COMMENT        (CObList #200=(AnlzFontBackColorItem 127 708 33619967) 
COMMENT         #201=(AnlzFontBackColorItem 709 1376 33554687))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     11556..15367
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(16466..17687)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     10105..11530
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     6464..10109
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     2510..6463
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    11537..11554
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1392..1425
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1426..1436
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1433..2152
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2153..2188
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    2189..2261
                     /vntifkey="21"
                     /label=NLS
     misc_feature    2267..2300
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    2454..2504
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    128..1377
                     /vntifkey="21"
                     /label=Ple79\(GABRA6-C)
                     /note="1306"
     primer          603..627
                     /vntifkey="27"
                     /label=oEMS2316
     primer          906..929
                     /vntifkey="27"
                     /label=oEMS2308
     misc_feature    128..709
                     /vntifkey="21"
                     /label=Region\4
     misc_feature    710..1377
                     /vntifkey="21"
                     /label=Prom\Region
BASE COUNT     4790 a      3919 c      3918 g      5066 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctctct tcaagccttc tccagtatga tctgattgga caaacagtat ctagtgagac 
      181 aattaaatct aacacaggta agaatttgac caaaggatgg aaataatccc tcaggatgac 
      241 tccagtgcac attttcaaaa tatctatttc tttttccttc gccttagcca ttctcagcta 
      301 agcatgctgt tttgcagtga tgagtagctt tccttaaaac tgattttgca accaggtgca 
      361 tataaagatt tgtgtaatct tggaataagt agtttttttt atgtatatga tataaagaac 
      421 cctactgaaa acaataagtt gatgtgtctc ttcgctctat catctgctga tcaagtagct 
      481 ttctcatata ctgaaatgta cctttgcttg ttttcttcca gtcaatgtta atttaaaagt 
      541 tgtcaagatt tatattcaac tgcattcttt ttgcagactg tcagcagtaa taatactgat 
      601 gtatgtgtct tccaacaggt gaatacgtta taatgacagt ttacttccac ttgcaaagga 
      661 agatgggcta cttcatgata cagatataca ctccttgcat tatgacagta tggccgtgag 
      721 ttgttctgtt gtgttacaag tgctggtaaa taaatcatga gcaataatct ttaaatacat 
      781 aagggtgtgg ctgtagctag taatgctgat gaaaatcaga aagggaatcc aagagggtca 
      841 caatagtgtt agacttttta aacagtaaga gctgtgtagg aatccttctg tgaaccataa 
      901 aaataataaa cgctgagcct attggaacat aatctcaatc agcaagatgc caatgctttc 
      961 cagatttcct cacagggccc tgctaattcc atgccaaaac tggcagtgga tttcttcctt 
     1021 ctaatctccc ttaatgcctt attcgattat tgtcactgct ctccgaccat gcactgtccc 
     1081 ttcagactct cctttcccaa ctgagtcagt cagaggagcc tgggtgtctg caggacataa 
     1141 tctaagacca caaaccacct tgttccacgt gagaaggaaa caagaaagaa gggagccagg 
     1201 ggaatcctgc aaatttttag gcaacctctt tatctattgg attactgact tgaggcaaac 
     1261 aaggaacaga gatatgaagg gtttgaaaga tttctccagt tgattggcag agaagagctg 
     1321 gctagcaggg aggacgaccc taggagggtg aattctgcat ttcagtgcac tgcaggaacg 
     1381 cgtggcgcgc cgaagttcct attcttcaaa aggtatagga acttccgcca ccatggtgag 
     1441 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 
     1501 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 
     1561 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 
     1621 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 
     1681 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 
     1741 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 
     1801 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 
     1861 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 
     1921 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 
     1981 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 
     2041 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 
     2101 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt tatttctgaa 
     2161 ctgctgcccg ggctgctgca tggaaccgga tccaaaaaag aagagaaagg tagatccaaa 
     2221 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta atataagaag ttcctattct 
     2281 ctagaaagta taggaacttc agcggccgcg actctagatc ataatcagcc ataccacatt 
     2341 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 
     2401 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 
     2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcgaattc tcgtaaaact 
     2521 cttcatggca gtagttatta ttctctctct ctctcttttt cttttttctt gagacaggat 
     2581 atttctctgt tgcccaggct ggagtgcagt ggcacagtct tggctcactg cagcctggac 
     2641 ctcctgggct caagccatcc tcccacctca gcctcccaag tagctggggc tacaggcaca 
     2701 tggccaccag gcccagataa tttttcattt ttgtagagac tgagtctcac catgttaccc 
     2761 aggtttatta ttctcatttt ttagatgaag agactgaggt ccagagaagc tcaatgactt 
     2821 gcctagtttt acaaatctcc tgccatcaca tacccctcag cgtccttaat aagagggagg 
     2881 ccaccaacta tgtgctgggc actgtggtgg atgctggagc tatagggttg agtatataag 
     2941 aaatggtgtt gctggagcaa ctgttgcttg cttacctgac ctatctgaga attaattagc 
     3001 aggggaacat atttttgttt tcagattcaa tataagaact tgtgtgggca aaaataaaga 
     3061 tcagtagtaa taacagtagt tcccatttgc tgactgtact gtcctaagtg catatatata 
     3121 tacatacaca cacgcatacc tatactcctc taatactcaa aatgatcctg tttatgtatt 
     3181 gttaatatgc tcattttatt tttaaatttt tatttatttt tatttttatt tatttttgag 
     3241 acggagtctc attctgtcgc ggaggctgaa gtgcagtggt gcgatctcag ctcagtgcga 
     3301 cctccgcctc ccgggttcaa gtgattctcc tgcctcagcc tccggactag ctgggattac 
     3361 aggcgcccgc ctccacgccc agctaatttt tgtattttta gtagagatgg ggtttcgcca 
     3421 tgttggccag gctggtctcg tactcctgac cttgagtgat ccacctgcct cggcctccca 
     3481 aagtgctggg attacaggca tgagccaccg cgccgggcta atatgctcat tttagtgagg 
     3541 caaaaataga ggctcagagt ctgatttgta caaaactaca gagcagttaa gtgtcctctc 
     3601 agatgtgtac cctgatctgg gtgactctag gactctaggt ctcaactgtt acaaccagtt 
     3661 aagggtttgg ggaagcactg ggccaagagt caggaaaatg gaagccacag gtagtgcaag 
     3721 gtcttgggaa tgggacgtct ggtccaagga ttcacgcgat gactggaacc cgaagagccg 
     3781 gggcccggtt tacggccgcc atgaagcaac gcgcgccggt aggtttggga atcagggagc 
     3841 cctctgaata ggagactgag ttgggaggga aaggggcttc gctgggggag cctcggcttc 
     3901 ttctgggaga aaattcccac ggctacctag tgagcctgca aactggtagg cgccggcgta 
     3961 ggcgcgcggg cggggccggg ggcggggcct gcggggcgtg gcggggcggg cagagggcgg 
     4021 ggcctgcttc tcctcagctt caggcggctg cgacgagccc tcaggcgaac ctctcggctt 
     4081 tcccgcgcgg cgccgcctct tgctgcgcct ccgcctcctc ctctgctccg ccaccggctt 
     4141 cctcctcctg agcagtcagc ccgcgcgccg gccggctccg ttatggcgac ccgcagccct 
     4201 ggcgtcgtgg tgagcagctc ggcctgccgg ccctggccgg ttcaggccca cgcggcaggt 
     4261 ggcggccggg ccctgaggcg cgggatcgat ccatgacacc cccccgcccc ccatatctca 
     4321 ctataattca ctgtcaaatg gcttttatga tttggagttt ggggatactt ttaaagacag 
     4381 ggtcttggta gctcctagat ggcttgggat tcacttagta acccttgctg gcctaaaatt 
     4441 caggaacttc tacctcatct tcccaagtac tgggttagta gggatgtcca gcatatttag 
     4501 cttgctttaa tgattttgct gtactttttt ctgtgtgtac aggtgcatgt atatttataa 
     4561 aactaacata atatatttta cccccaaagt accataaccc taattaatgc tattaaatgt 
     4621 tttgtatgct gggctctggg catactaata taaccaatat tatacaatgc aaaacaattt 
     4681 tggaatatct tctgtaaaat tatcttatta gtaagttgta accatctcag acttctaagt 
     4741 ttgagtttgt aaaggatcag ttttttttgg tgacctacac acacacacac acacacacac 
     4801 acacacacac acacaggggg gtggattctg agaatttaga gaatttagat ggttaatacc 
     4861 agtttttatt tttattctgt gtgatattta tttattaaga tttatttatt tattacatgt 
     4921 aggtacactg tatctgtctt cagacacacc agaagagggc atcagatctc gttacagatg 
     4981 gttgtgagtc accatgtggt tgctgggatt tgaactcagg acctctggaa gaacagtcag 
     5041 tgctcttaac cgttgagcca tctcaccagc cccgtgatat tttatttaac ttactagaat 
     5101 atactgtgtt aaaacatatt ttaggggatt ggggagatgg cccattctgt aaagtgtctg 
     5161 ccatgtcatc ttcaggacct gcgtttgtaa ccccagcatg gagaagacag agacagggag 
     5221 acccctgggc ttcctggcaa gctagactag caaatttggt taactaggtt caatgagaga 
     5281 ctcagtctca aaacataaga agtgaatttt ttagacccaa tttcaatctc tgaactccat 
     5341 acgtatgcac atgtacctgc atataaatat atatacacac ccataaacac atgcacacac 
     5401 aaaaaatggt tcaaaagttc ttaaaatgta taagagtaga aagtgtagag ccgggagtgg 
     5461 tggcgcacgc ctttaatccc agcacttggg agcagaggca ggtggatttc tgagttcgag 
     5521 gccagcctgg tctacagagt gagttccagg acagccagag ctatacagag aaatcctgcc 
     5581 tcaaaaaacc aaaaataaag tgtatatact aatctttcat gcctaaaata tttatttttg 
     5641 ccacattttt catacctctt taatattttt ctttttctct ttgtgcttca aaggaaattt 
     5701 tgtgtcatgt tttacttttc attcttgact ctattcagca gtaagacgca gcatggcttt 
     5761 cttagagaag tataatctat tacaaaacgg taacatttaa ctgctctaca taccctcttg 
     5821 tagctaaatt cctctgttaa actaagactt attatctaaa cgtaattaaa tgttgatatc 
     5881 ttataatgct ccactttgaa acagctgtct ttcttttgca gattagcgat gatgaaccag 
     5941 gttatgacct agatttgttt tgtataccta atcattatgc cgaggatttg gaaaaagtgt 
     6001 ttattcctca tggactgatt atggacaggt tagtaagacc tcgattgaag ttatttttag 
     6061 agggtgtgtg tgtggtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtaagaga 
     6121 gagaggagag gcatgtgtga tatatatgtg aagtgtattg catgtacatg gaatccagag 
     6181 aaggacatta ggtgtcctac tctgttgctc tcttattccc ttgaatcagt gtctcttgct 
     6241 gaacctgggg catacatact gatttggcta ggctgcctgg ctagcaagac ccagggatct 
     6301 gcttgtctct gccccccagt gctgacgtta caagcaggtg caaccacatc tggtttttat 
     6361 atgggtactg ggggatctga acttagggcc tgatgcgtgc ccaagtgctc tcacccgcca 
     6421 agccacctct ccagccaaag ggtttttttt cttgtattaa ttctaactta gcaactgttg 
     6481 agtagttgat gtttgaatat aagctaagcc aacttattag aataaatgtt ttctattttt 
     6541 tacttgtatg tgtgagtgcc tgcatgtatg tatgtgaagc atatgtattc caggatgtca 
     6601 gaattgtgat ggattccctg gaactggagt tacagaccat tgtgaaccac tgtgtatggg 
     6661 tgctgggaac tgaactttag gtcctctgca aaaacagtaa ggatcttaac tgataaacta 
     6721 tctttctagc cctaaggctt tctactttta aagttcgttt ctctttctct ctctctctct 
     6781 ctctctctct ctctctctcc ctctccctcc ctcctcctcc tcctcctctt tcccccctct 
     6841 cgcctcctct ttctctctcc cctccctctc tctctctcac acacacacac agacagagag 
     6901 aggagagaga aaggaagcct tactaattaa ttacctagtt ttagacataa ctagtgggaa 
     6961 tccatagtaa gtaaatcaac attcttgaag agaggttaga agaattggct ctcgttgtcc 
     7021 atcgaggctg tataagatta ttcttctgtt ttctcagctt accatacata ttttctacgt 
     7081 agacaatacc aattcaaata cactgtacgt aaagtatatg cgcacacaca cacacacaca 
     7141 cacacacaca cacacacaag ttacatattg tcatatagta ctaaaattta tggacttttt 
     7201 catattaata catctagagc tatcatgttt tttatctttt aaaaattact ttacggggct 
     7261 acagagatgt ctcagcagtt aggagcactg actgctcttc cagaggtcct gagttcaatt 
     7321 cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatcca ctcttatggt 
     7381 gtgtctgaag acagtgacag tgtactcata tacatgaaat aaatctttaa aaaattactt 
     7441 taccatcagg gtatatatat atatatatat gtgtgtgtgt gtgtgtgtgt gtatgaagta 
     7501 tttgctattt gagagggtct cactgtttag ctcagtctgg cttggaacgt tctgtatagc 
     7561 ccagactggc ctcaactcag cagtccccct gaaaggctct catatgtgga gattacaggt 
     7621 atgtgctact gtgcccagcc ctacatgtta ctttgttttt gaaatagggt atcaaatagc 
     7681 ttagactggc ttctaagtca gtgtgtagct aaggatggtc tgagcttctg aacctgctac 
     7741 ttcaactcct ggtgtgccat catggccagt ttgcacagtt gtgagagtag aactcaggac 
     7801 ctcctatgtg ttactagaca tgttctctac cagctgagct acatcccaat tcgcctacat 
     7861 ttacttttat tttttaaaag atttatttat gtatacagca ctctgcctgc atgtacatct 
     7921 gcatgccaga agagggcacc agatctcatc atacatagtt gtgagccacc atgtggttgc 
     7981 tggggattga actcagggcc tctggaagag cagtcagtgc tcttaacctc tgagccatct 
     8041 ctgtagcccc ctacatttat tttaaagatt ataggctatt ctattcatag atgtgccata 
     8101 actgatttaa ccagatcttc attattttgt acttagtaga gtatattaga gggaatgaat 
     8161 cttaaataca cattttgaca cttgctcagc tttagacaag taaaaatcac tggtcaagga 
     8221 aatggtgctt agtggatgct tctgaactgt caaattgtcc tccaacaagt tacatcattt 
     8281 tatacttcca acgttatgtg agagttcatt tttctttagg ctcttctagt attttatatt 
     8341 tgagaaataa aatttaacca aaatttgcca atctagtaag cttgttttta tttgtatctt 
     8401 taaattttat gatagatgtt atagtgtact ctcctctccc ctcccctccc ctctcctcct 
     8461 cagcaggatc ttgttatgta gccctaaggg atctaatact tactctgtag tccaggctgt 
     8521 ccttcagctc atgagacaca ccatcatgct tggcatgttt cttgttctga aaggtatctg 
     8581 gttttaattt tggttgagct ggtgtgttgg tgtatatctg taatctcagc acaggcaagt 
     8641 agagacaagg attatgatta cagtccagcc tgggctattt agagggattc tctttaatct 
     8701 ttcctcatgc cccaaaatct tacctttggt atatgaaaaa tagtctccac ttctgcaaaa 
     8761 tattgcttta tgaagtaaga attaattccc ttcatagaga caaggaatgt gtcctgtaaa 
     8821 agtttaatgt gtaagaagta tttgttataa aagataaata ttcagaatct tctttttaat 
     8881 tcctgatttt atttctatag gactgaaaga cttgctcgag atgtcatgaa ggagatggga 
     8941 ggccatcaca ttgtggccct ctgtgtgctc aaggggggct ataagttctt tgctgacctg 
     9001 ctggattaca ttaaagcact gaatagaaat agtgatagat ccattcctat gactgtagat 
     9061 tttatcagac tgaagagcta ctgtgtaagt ataattaact tataattaaa aaaatagggc 
     9121 cattctagtt ttatctatat tttttttaaa cttgtgcaaa ctatgctaca taatcaactt 
     9181 acttgattat gtaatatata tagtaatagt tggaattact attattacat atttattttt 
     9241 ctgtgctggg gtagaaccca gggaactaag tactgtaata accgagcttc attcatccta 
     9301 gtcattttta attttttttt ttttttttgt tttttcgaga caaggtttct ctgtgtagcc 
     9361 ctggctgtcc tggaactcac tctgtagacc aggctggcct cgaactcaga aatctgcctg 
     9421 cctctgcctc ccagtgctga gattaatgac gtgcaccacc actgcctggc atttttttaa 
     9481 agttttaatt tgagaccctt tctcactaac ttgctcacac tatcctcgaa ctcattacgt 
     9541 gggctggtct agaaatttca gtcaacttgc ctcagcttct caagtagctg ggattacagg 
     9601 caggtgctac cacttctggc catgatcatt gttgttgttg ctattattat tattattatt 
     9661 tcactgatac ttcatatcac aacacagaat agtaactgta gttcatgacc aacaaaaaag 
     9721 ttgtacagtt tctctgttgt cttattttga tctggatttt gatttgtttc atggcactgg 
     9781 gcaggctaat ctcaaactgc atggctcaaa tagtccttct gtcttaatct cccatgttct 
     9841 ggaagtatag gtatcactac acctagctgt attttcatat tttaaaaaaa acgcctaaga 
     9901 attgctattg aatgaggcgg agagatggct cagcagtcac aagtacttgt agaggactgg 
     9961 ggtttggtta ctagcacctc aaagatggct tacaattatc tgtagctcca attccagagg 
    10021 atctgtgtgg taaagctgca tgtatatggg catgcaggta aaactgctaa acatataaaa 
    10081 atagctcagt cttaagagaa ttggtcgact ttttcgccct ttgacgttgg agtccacgtt 
    10141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
    10201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
    10261 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
    10321 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
    10381 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
    10441 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
    10501 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
    10561 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
    10621 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
    10681 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
    10741 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
    10801 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
    10861 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
    10921 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
    10981 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
    11041 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
    11101 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
    11161 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
    11221 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
    11281 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
    11341 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
    11401 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
    11461 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
    11521 ccgctaccag gtcgagtagg gataacaggg taatggatcc tcgttgggca cctgcactca 
    11581 tgtacgcagg cccacatata gatgtacaca cgcatatcat tcaaaataga aattatttat 
    11641 acaagtagtg cgttggagag ctggctcagg agttgttaat gcttgctacc cttgcaaagg 
    11701 acccaagttc aatttccagc acctgtgttg ggcatctcac aaccacatgt aaatctagtt 
    11761 tcaggggatt tcacccctct tctgaactct tcaggcactt atacccacaa atggacatct 
    11821 ccacatagag acacatactt ttaaaaaagt aatctagggt ctagagaaat ggttatagac 
    11881 cacggactgg gatcagttcc cagaagccac atcctgtggc tcacaattgc ctgtgaatca 
    11941 agttctagat ctggtggcct cgtctagcct gcagtcatca acaaacacat gcacacatcc 
    12001 atacacacat aatttaaaag aaagcgaaag taacctataa tgctaaacaa agcacattct 
    12061 cagtgtagac tgcagtggtg atgaaccttg aaaatatatt aatagaagcc agacacgtaa 
    12121 gtcacaaatg gtataactca atttatatga aattcccaga gagtccaaag ttaaaaaaaa 
    12181 caaaaaggca gattattggt tgtcagtagc tacagagatg ggacattgaa gatattaata 
    12241 ggtatagggt tgaggattca tggcctttcc ctcttccatg ttagcatgtc tattgttgtt 
    12301 aaccttattc aactcatgtt agggcaatca tattgatgag acttaatgag tatagcttga 
    12361 cattcctaga agacagagtc tcacatcaga ctctctgatc ctctgactct tagaatcttt 
    12421 caacaccctc ttctgaaatg atctctgagc ctttggttgt gactgaatta tgagaaagaa 
    12481 ttttatgttg aatgaatttt acttcagcat ttttacaaaa ctgaagctgg ggatggcgtt 
    12541 tcatgcgtgc agtccctacc atttgcctat aagcctggag gaggcagaag tagaattagg 
    12601 cttttcaggg acatcttcag ctgcatcatg agcttgagga caacttgagc tacataagat 
    12661 tcccatcccc acccccacac acacacacac atctagtatg tacctagtat gcagtctttt 
    12721 catatgttta aaatgtagaa aggtatatgt gaagtctccc ttttccccac aacttagtcc 
    12781 ctccaaagta ttgcatcagt ttccttgtac atgattttat gcatatataa gatacactat 
    12841 ttctctctct ctctctctcc ttccctcctt ccctccaacc tctgggtatg agttgagggt 
    12901 tatgtatgtt tcaagttgta ataagaattg ccactcaaac atttcatccc attttaagtt 
    12961 aagtttgtct gattttccac tttagaaggg cagccatgat ggctcatgcc tataatccca 
    13021 tcctagcact tgtaagccta atacataaga ctggaggatt gtcatcagct tgaggctagc 
    13081 ctggtctacc taatgagttc cagccagcca agagtagaat ataagaccct gtctccaaaa 
    13141 aatgtaccaa aggggctgga ggggtaactc agtggttaag aacagtagct gctcttccag 
    13201 aggacctggg ttcaattccc agcacccaca tggcagcttg caactatctg taactgtcca 
    13261 gctttgggat atccgacacc attataccaa ttacataaaa tgaaaaaatt gtaaaatgtg 
    13321 ctaaaatgag tggggagggg gagcatggtg tggttctgtg ggcttgggat tttgccatga 
    13381 atccatgact atatctcagc aatatgtgga ttatttctgg aaacctgtgg ggcatgggtg 
    13441 tatgggaaga ggggagagag agagagcccg catccagcca gagttcctgt gctctgggca 
    13501 ggcggacgtg ggaggactgc tggacgattt ccacttggcc ctgggtgggc atctggctgt 
    13561 gtgaagccac tgaccccact cagcgggggg tagataaggg gcatttccca taccaggttc 
    13621 tcagactcca gcattccacg ctggatacag cagaagagct gagaacagaa taggggcccc 
    13681 agggagacac tctcgtccca gagataaaga aggcagaggg agagagattc ctacgcaggc 
    13741 gagaggcctt agtctgggcc ttgatgaaca gagacggtct atggttttag agctttattg 
    13801 tagaaaggca gggggaaaga gagaaggtag aaagagagag accagccatg gccaagagga 
    13861 gagaaggggg gaaaggagag agagaagaaa ggctagagag taagaaaggt taaagagaca 
    13921 gaagtaaaag cttagagtaa gagagagaga gagagcaaga gggagagtaa gaaaagtaaa 
    13981 agcttaagag agcgaggagg ggccagcccc tctaatagtg ggcttgttat cttgttgttg 
    14041 ctaggtaact gtgaagcgtc tagcctgaag gccagaagct tgggacattg tctatgtgac 
    14101 tgaaagccat gagcctctac tgtgggggct gtggaggtgg taacttggac aggagccagg 
    14161 ggttccagga gacatgaggg aatgccttct gtcccatgta ggtaggaatt accaccaccg 
    14221 gctttcaccg tagttcactg gagactgttt gtacatagcc cattgcccca cagaaaccag 
    14281 gcactcactg tttcatttgc ataagaatta gaaggcttta ttttggctgg gattggtgac 
    14341 ttgccccaga aattccagct tttgggaagc cgaatcctgg agaaccagga gttcaaggtc 
    14401 atcctcaggt acctcatttg ttcaagacca gcctgtgcta ccatgagacc ttgtccctac 
    14461 tcccctcaac cccaaaacac gctgttttca ttttataggt tgtactaacc atggaggatg 
    14521 gtcatttgcc tttctcttga tttcagaagc gtagcgcggg ctttgcacca tgatctagtg 
    14581 tttccagata ggcttgcctc tccacagcca ggcttatcct gcttcagtcc catgtttggc 
    14641 aagggcaaca tatgactgaa gaacttgcat agccttatgg tgtatcatga ctcttagccc 
    14701 cagtttataa ctgtctaaca gctggtggct tttgtgaaaa aggaaagcat catttgaact 
    14761 tttaactttt cagctgtttg ttttgacttt gcataaatac tgagactagc taggaaatga 
    14821 gcttttgttt catttgctaa aaatgatttt tttttcatga ggcaatttca agcaatttga 
    14881 atttagcaat ctctacttgt ctgaaaagaa gttattaatt aaaaaaaaac aaacaaacag 
    14941 gaacctatta tgctggctag tcacatgaaa gaatccaaat gaacacagtc aaggctgaat 
    15001 caacacatga aattattttc taattcccca aattacattt atgctaatta gtatgtagta 
    15061 aaacatttta attgtttcca atgttaaaca ttacagaaat aattttgatg ttttcctaat 
    15121 aacccagcct ttgatcatgt ttgcatttat ttgattgttg tcagtttatt aaataattaa 
    15181 gtaagagcaa caagataaaa tgacttcatg agagtccttt tctgtaactt agaacgtcag 
    15241 tagtcatagg aactgcggtc gttcccttac ctttcagaag taggctaaag agttgaacgc 
    15301 aaaggtggtt tttcgaatct tcctgatcct taagccccca ctaggtttat attctatatt 
    15361 tgatatcgga ttcgaacccc tcgagcttag gatccggaac ccttaatata actaagttat 
    15421 taggtccctc gaagaggttc actagtactg gccattgcgg ccgcttcgag gctgcctcgc 
    15481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
    15541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
    15601 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 
    15661 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 
    15721 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 
    15781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
    15841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
    15901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
    15961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
    16021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
    16081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
    16141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
    16201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
    16261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
    16321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
    16381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
    16441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
    16501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
    16561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
    16621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
    16681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
    16741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
    16801 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
    16861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
    16921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
    16981 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 
    17041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
    17101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
    17161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
    17221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
    17281 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 
    17341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
    17401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
    17461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
    17521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
    17581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
    17641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgg 
//
