LOCUS       Ple30\(pEMS1158)       17270 bp    DNA     circular     26-JAN-2009
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364  5'- GCGTATCACGAGGCCCTTTC -3'  Tm= 56.0 Deg C
            Antisense primer: oEMS2611  5'- CAGGGCAAAGTCAACAGTGTCAC -3' Tm=56.0 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            727 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|508254700|
COMMENT     VNTDBDATE|508254700|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple30 (pEMS1158)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT                      #186=(CGroupWidget 
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COMMENT                             #189=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.00584 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                            (CObjectList)) 
COMMENT                      #191=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -116 100) 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   -10 10.4901 1.17389 #192#) 
COMMENT                             #194=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #115# 0 100) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 8.60511 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #194#) 
COMMENT                             #196=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #115# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 4.0792 
COMMENT                                    5.51546 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.5376 
COMMENT                                   -3.48466 0 -10 14.9609 1.17389 #196#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #198=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -256 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #199=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -186 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #200=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -326 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #201=(AnlzFontBackColorItem 53 72 43633766) 
COMMENT         #202=(AnlzFontBackColorItem 138 501 40304639) 
COMMENT         #203=(AnlzFontBackColorItem 502 584 33606707) 
COMMENT         #204=(AnlzFontBackColorItem 585 735 50318387) 
COMMENT         #205=(AnlzFontBackColorItem 759 959 50318387) 
COMMENT         #206=(AnlzFontBackColorItem 736 758 43633766))) (CObList) 17498167 
COMMENT       (CStringList) 0 851972 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     11133..14944
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(16043..17264)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     9682..11107
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     6041..9686
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     2087..6040
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    11114..11131
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    969..1002
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1003..1013
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1010..1729
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    1730..1765
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    1766..1838
                     /vntifkey="21"
                     /label=NLS
     misc_feature    1844..1877
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    2031..2081
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..960
                     /vntifkey="21"
                     /label=Ple30\(CD68-A)
                     /note="1306"
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     primer          737..759
                     /vntifkey="27"
                     /label=oEMS2611
     misc_feature    139..502
                     /vntifkey="21"
                     /label=Region\1
     misc_feature    503..585
                     /vntifkey="21"
                     /label=Region\2\(upside\down)
     misc_feature    586..960
                     /vntifkey="21"
                     /label=Prom\region
BASE COUNT     4614 a      3889 c      3882 g      4885 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccag ggatgatgag tataatccaa ggaccagaga agtcttctct 
      181 gatcaccacc ttgggaggaa gacatgggtg ccctaacact ctcgagacct gctgggttaa 
      241 ttaaaagcta tttcttaccc aaacgtaacc attgcttcct ccacccattt cctgagtcaa 
      301 atgggaaagc tgttgggtga agcctggctg gctgggcaag tttgactgtg ttctgaataa 
      361 gcaccttcac tatgggctaa gagatccctt ggtgtggggg tgatcttaca gtagtcagag 
      421 cagatggaca gtccttttca cccttgctta atagccagag ctgtttcatg cctggggcac 
      481 acacaattct aatgctggac ttcttttggc agagaggaga tggttagcaa cccagggcag 
      541 gctccctccc aggggccccc tcccctcagc ctccttcctc cttacgaaag gagtggaaac 
      601 cgatttcccc accaagggag gggcctgtac ctcagctgtt cccatagcta cttgccacaa 
      661 ctgccaagca agtttcgctg agtttgacac atggatccct gtggatcaac tgccctagga 
      721 ctccgtttgc acccatgtga cactgttgac tttgccctga tgaagcaggg ccaacagtcc 
      781 cctaacttaa ttacaaaaac taatgactaa gagagaggtg gctagagctg aggcccctga 
      841 gtcaggctgt gggtgggatc atctccagta caggaagtga gactttcatt tcctcctttc 
      901 caagagaggg ctgagggagc agggttgagc aactggtgca gacagcctag ctggactttg 
      961 ggcgcgccga agttcctatt cttcaaaagg tataggaact tccgccacca tggtgagcaa 
     1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 
     1081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 
     1141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 
     1201 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 
     1261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 
     1321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 
     1381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 
     1441 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 
     1501 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 
     1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 
     1621 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 
     1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagttat ttctgaactg 
     1741 ctgcccgggc tgctgcatgg aaccggatcc aaaaaagaag agaaaggtag atccaaaaaa 
     1801 gaagagaaag gtagatccaa aaaagaagag aaaggtaata taagaagttc ctattctcta 
     1861 gaaagtatag gaacttcagc ggccgcgact ctagatcata atcagccata ccacatttgt 
     1921 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 
     1981 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 
     2041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cgaattctcg taaaactctt 
     2101 catggcagta gttattattc tctctctctc tctttttctt ttttcttgag acaggatatt 
     2161 tctctgttgc ccaggctgga gtgcagtggc acagtcttgg ctcactgcag cctggacctc 
     2221 ctgggctcaa gccatcctcc cacctcagcc tcccaagtag ctggggctac aggcacatgg 
     2281 ccaccaggcc cagataattt ttcatttttg tagagactga gtctcaccat gttacccagg 
     2341 tttattattc tcatttttta gatgaagaga ctgaggtcca gagaagctca atgacttgcc 
     2401 tagttttaca aatctcctgc catcacatac ccctcagcgt ccttaataag agggaggcca 
     2461 ccaactatgt gctgggcact gtggtggatg ctggagctat agggttgagt atataagaaa 
     2521 tggtgttgct ggagcaactg ttgcttgctt acctgaccta tctgagaatt aattagcagg 
     2581 ggaacatatt tttgttttca gattcaatat aagaacttgt gtgggcaaaa ataaagatca 
     2641 gtagtaataa cagtagttcc catttgctga ctgtactgtc ctaagtgcat atatatatac 
     2701 atacacacac gcatacctat actcctctaa tactcaaaat gatcctgttt atgtattgtt 
     2761 aatatgctca ttttattttt aaatttttat ttatttttat ttttatttat ttttgagacg 
     2821 gagtctcatt ctgtcgcgga ggctgaagtg cagtggtgcg atctcagctc agtgcgacct 
     2881 ccgcctcccg ggttcaagtg attctcctgc ctcagcctcc ggactagctg ggattacagg 
     2941 cgcccgcctc cacgcccagc taatttttgt atttttagta gagatggggt ttcgccatgt 
     3001 tggccaggct ggtctcgtac tcctgacctt gagtgatcca cctgcctcgg cctcccaaag 
     3061 tgctgggatt acaggcatga gccaccgcgc cgggctaata tgctcatttt agtgaggcaa 
     3121 aaatagaggc tcagagtctg atttgtacaa aactacagag cagttaagtg tcctctcaga 
     3181 tgtgtaccct gatctgggtg actctaggac tctaggtctc aactgttaca accagttaag 
     3241 ggtttgggga agcactgggc caagagtcag gaaaatggaa gccacaggta gtgcaaggtc 
     3301 ttgggaatgg gacgtctggt ccaaggattc acgcgatgac tggaacccga agagccgggg 
     3361 cccggtttac ggccgccatg aagcaacgcg cgccggtagg tttgggaatc agggagccct 
     3421 ctgaatagga gactgagttg ggagggaaag gggcttcgct gggggagcct cggcttcttc 
     3481 tgggagaaaa ttcccacggc tacctagtga gcctgcaaac tggtaggcgc cggcgtaggc 
     3541 gcgcgggcgg ggccgggggc ggggcctgcg gggcgtggcg gggcgggcag agggcggggc 
     3601 ctgcttctcc tcagcttcag gcggctgcga cgagccctca ggcgaacctc tcggctttcc 
     3661 cgcgcggcgc cgcctcttgc tgcgcctccg cctcctcctc tgctccgcca ccggcttcct 
     3721 cctcctgagc agtcagcccg cgcgccggcc ggctccgtta tggcgacccg cagccctggc 
     3781 gtcgtggtga gcagctcggc ctgccggccc tggccggttc aggcccacgc ggcaggtggc 
     3841 ggccgggccc tgaggcgcgg gatcgatcca tgacaccccc ccgcccccca tatctcacta 
     3901 taattcactg tcaaatggct tttatgattt ggagtttggg gatactttta aagacagggt 
     3961 cttggtagct cctagatggc ttgggattca cttagtaacc cttgctggcc taaaattcag 
     4021 gaacttctac ctcatcttcc caagtactgg gttagtaggg atgtccagca tatttagctt 
     4081 gctttaatga ttttgctgta cttttttctg tgtgtacagg tgcatgtata tttataaaac 
     4141 taacataata tattttaccc ccaaagtacc ataaccctaa ttaatgctat taaatgtttt 
     4201 gtatgctggg ctctgggcat actaatataa ccaatattat acaatgcaaa acaattttgg 
     4261 aatatcttct gtaaaattat cttattagta agttgtaacc atctcagact tctaagtttg 
     4321 agtttgtaaa ggatcagttt tttttggtga cctacacaca cacacacaca cacacacaca 
     4381 cacacacaca caggggggtg gattctgaga atttagagaa tttagatggt taataccagt 
     4441 ttttattttt attctgtgtg atatttattt attaagattt atttatttat tacatgtagg 
     4501 tacactgtat ctgtcttcag acacaccaga agagggcatc agatctcgtt acagatggtt 
     4561 gtgagtcacc atgtggttgc tgggatttga actcaggacc tctggaagaa cagtcagtgc 
     4621 tcttaaccgt tgagccatct caccagcccc gtgatatttt atttaactta ctagaatata 
     4681 ctgtgttaaa acatatttta ggggattggg gagatggccc attctgtaaa gtgtctgcca 
     4741 tgtcatcttc aggacctgcg tttgtaaccc cagcatggag aagacagaga cagggagacc 
     4801 cctgggcttc ctggcaagct agactagcaa atttggttaa ctaggttcaa tgagagactc 
     4861 agtctcaaaa cataagaagt gaatttttta gacccaattt caatctctga actccatacg 
     4921 tatgcacatg tacctgcata taaatatata tacacaccca taaacacatg cacacacaaa 
     4981 aaatggttca aaagttctta aaatgtataa gagtagaaag tgtagagccg ggagtggtgg 
     5041 cgcacgcctt taatcccagc acttgggagc agaggcaggt ggatttctga gttcgaggcc 
     5101 agcctggtct acagagtgag ttccaggaca gccagagcta tacagagaaa tcctgcctca 
     5161 aaaaaccaaa aataaagtgt atatactaat ctttcatgcc taaaatattt atttttgcca 
     5221 catttttcat acctctttaa tatttttctt tttctctttg tgcttcaaag gaaattttgt 
     5281 gtcatgtttt acttttcatt cttgactcta ttcagcagta agacgcagca tggctttctt 
     5341 agagaagtat aatctattac aaaacggtaa catttaactg ctctacatac cctcttgtag 
     5401 ctaaattcct ctgttaaact aagacttatt atctaaacgt aattaaatgt tgatatctta 
     5461 taatgctcca ctttgaaaca gctgtctttc ttttgcagat tagcgatgat gaaccaggtt 
     5521 atgacctaga tttgttttgt atacctaatc attatgccga ggatttggaa aaagtgttta 
     5581 ttcctcatgg actgattatg gacaggttag taagacctcg attgaagtta tttttagagg 
     5641 gtgtgtgtgt ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taagagagag 
     5701 aggagaggca tgtgtgatat atatgtgaag tgtattgcat gtacatggaa tccagagaag 
     5761 gacattaggt gtcctactct gttgctctct tattcccttg aatcagtgtc tcttgctgaa 
     5821 cctggggcat acatactgat ttggctaggc tgcctggcta gcaagaccca gggatctgct 
     5881 tgtctctgcc ccccagtgct gacgttacaa gcaggtgcaa ccacatctgg tttttatatg 
     5941 ggtactgggg gatctgaact tagggcctga tgcgtgccca agtgctctca cccgccaagc 
     6001 cacctctcca gccaaagggt tttttttctt gtattaattc taacttagca actgttgagt 
     6061 agttgatgtt tgaatataag ctaagccaac ttattagaat aaatgttttc tattttttac 
     6121 ttgtatgtgt gagtgcctgc atgtatgtat gtgaagcata tgtattccag gatgtcagaa 
     6181 ttgtgatgga ttccctggaa ctggagttac agaccattgt gaaccactgt gtatgggtgc 
     6241 tgggaactga actttaggtc ctctgcaaaa acagtaagga tcttaactga taaactatct 
     6301 ttctagccct aaggctttct acttttaaag ttcgtttctc tttctctctc tctctctctc 
     6361 tctctctctc tctctccctc tccctccctc ctcctcctcc tcctctttcc cccctctcgc 
     6421 ctcctctttc tctctcccct ccctctctct ctctcacaca cacacacaga cagagagagg 
     6481 agagagaaag gaagccttac taattaatta cctagtttta gacataacta gtgggaatcc 
     6541 atagtaagta aatcaacatt cttgaagaga ggttagaaga attggctctc gttgtccatc 
     6601 gaggctgtat aagattattc ttctgttttc tcagcttacc atacatattt tctacgtaga 
     6661 caataccaat tcaaatacac tgtacgtaaa gtatatgcgc acacacacac acacacacac 
     6721 acacacacac acacaagtta catattgtca tatagtacta aaatttatgg actttttcat 
     6781 attaatacat ctagagctat catgtttttt atcttttaaa aattacttta cggggctaca 
     6841 gagatgtctc agcagttagg agcactgact gctcttccag aggtcctgag ttcaattccc 
     6901 agcaaccaca tggtggctca caaccatctg taatgggatc tgatccactc ttatggtgtg 
     6961 tctgaagaca gtgacagtgt actcatatac atgaaataaa tctttaaaaa attactttac 
     7021 catcagggta tatatatata tatatatgtg tgtgtgtgtg tgtgtgtgta tgaagtattt 
     7081 gctatttgag agggtctcac tgtttagctc agtctggctt ggaacgttct gtatagccca 
     7141 gactggcctc aactcagcag tccccctgaa aggctctcat atgtggagat tacaggtatg 
     7201 tgctactgtg cccagcccta catgttactt tgtttttgaa atagggtatc aaatagctta 
     7261 gactggcttc taagtcagtg tgtagctaag gatggtctga gcttctgaac ctgctacttc 
     7321 aactcctggt gtgccatcat ggccagtttg cacagttgtg agagtagaac tcaggacctc 
     7381 ctatgtgtta ctagacatgt tctctaccag ctgagctaca tcccaattcg cctacattta 
     7441 cttttatttt ttaaaagatt tatttatgta tacagcactc tgcctgcatg tacatctgca 
     7501 tgccagaaga gggcaccaga tctcatcata catagttgtg agccaccatg tggttgctgg 
     7561 ggattgaact cagggcctct ggaagagcag tcagtgctct taacctctga gccatctctg 
     7621 tagcccccta catttatttt aaagattata ggctattcta ttcatagatg tgccataact 
     7681 gatttaacca gatcttcatt attttgtact tagtagagta tattagaggg aatgaatctt 
     7741 aaatacacat tttgacactt gctcagcttt agacaagtaa aaatcactgg tcaaggaaat 
     7801 ggtgcttagt ggatgcttct gaactgtcaa attgtcctcc aacaagttac atcattttat 
     7861 acttccaacg ttatgtgaga gttcattttt ctttaggctc ttctagtatt ttatatttga 
     7921 gaaataaaat ttaaccaaaa tttgccaatc tagtaagctt gtttttattt gtatctttaa 
     7981 attttatgat agatgttata gtgtactctc ctctcccctc ccctcccctc tcctcctcag 
     8041 caggatcttg ttatgtagcc ctaagggatc taatacttac tctgtagtcc aggctgtcct 
     8101 tcagctcatg agacacacca tcatgcttgg catgtttctt gttctgaaag gtatctggtt 
     8161 ttaattttgg ttgagctggt gtgttggtgt atatctgtaa tctcagcaca ggcaagtaga 
     8221 gacaaggatt atgattacag tccagcctgg gctatttaga gggattctct ttaatctttc 
     8281 ctcatgcccc aaaatcttac ctttggtata tgaaaaatag tctccacttc tgcaaaatat 
     8341 tgctttatga agtaagaatt aattcccttc atagagacaa ggaatgtgtc ctgtaaaagt 
     8401 ttaatgtgta agaagtattt gttataaaag ataaatattc agaatcttct ttttaattcc 
     8461 tgattttatt tctataggac tgaaagactt gctcgagatg tcatgaagga gatgggaggc 
     8521 catcacattg tggccctctg tgtgctcaag gggggctata agttctttgc tgacctgctg 
     8581 gattacatta aagcactgaa tagaaatagt gatagatcca ttcctatgac tgtagatttt 
     8641 atcagactga agagctactg tgtaagtata attaacttat aattaaaaaa atagggccat 
     8701 tctagtttta tctatatttt ttttaaactt gtgcaaacta tgctacataa tcaacttact 
     8761 tgattatgta atatatatag taatagttgg aattactatt attacatatt tatttttctg 
     8821 tgctggggta gaacccaggg aactaagtac tgtaataacc gagcttcatt catcctagtc 
     8881 atttttaatt tttttttttt tttttgtttt ttcgagacaa ggtttctctg tgtagccctg 
     8941 gctgtcctgg aactcactct gtagaccagg ctggcctcga actcagaaat ctgcctgcct 
     9001 ctgcctccca gtgctgagat taatgacgtg caccaccact gcctggcatt tttttaaagt 
     9061 tttaatttga gaccctttct cactaacttg ctcacactat cctcgaactc attacgtggg 
     9121 ctggtctaga aatttcagtc aacttgcctc agcttctcaa gtagctggga ttacaggcag 
     9181 gtgctaccac ttctggccat gatcattgtt gttgttgcta ttattattat tattatttca 
     9241 ctgatacttc atatcacaac acagaatagt aactgtagtt catgaccaac aaaaaagttg 
     9301 tacagtttct ctgttgtctt attttgatct ggattttgat ttgtttcatg gcactgggca 
     9361 ggctaatctc aaactgcatg gctcaaatag tccttctgtc ttaatctccc atgttctgga 
     9421 agtataggta tcactacacc tagctgtatt ttcatatttt aaaaaaaacg cctaagaatt 
     9481 gctattgaat gaggcggaga gatggctcag cagtcacaag tacttgtaga ggactggggt 
     9541 ttggttacta gcacctcaaa gatggcttac aattatctgt agctccaatt ccagaggatc 
     9601 tgtgtggtaa agctgcatgt atatgggcat gcaggtaaaa ctgctaaaca tataaaaata 
     9661 gctcagtctt aagagaattg gtcgactttt tcgccctttg acgttggagt ccacgttctt 
     9721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     9781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     9841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 
     9901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     9961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
    10021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
    10081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
    10141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
    10201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
    10261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
    10321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
    10381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
    10441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
    10501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
    10561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
    10621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
    10681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
    10741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
    10801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
    10861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
    10921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
    10981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
    11041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
    11101 ctaccaggtc gagtagggat aacagggtaa tggatcctcg ttgggcacct gcactcatgt 
    11161 acgcaggccc acatatagat gtacacacgc atatcattca aaatagaaat tatttataca 
    11221 agtagtgcgt tggagagctg gctcaggagt tgttaatgct tgctaccctt gcaaaggacc 
    11281 caagttcaat ttccagcacc tgtgttgggc atctcacaac cacatgtaaa tctagtttca 
    11341 ggggatttca cccctcttct gaactcttca ggcacttata cccacaaatg gacatctcca 
    11401 catagagaca catactttta aaaaagtaat ctagggtcta gagaaatggt tatagaccac 
    11461 ggactgggat cagttcccag aagccacatc ctgtggctca caattgcctg tgaatcaagt 
    11521 tctagatctg gtggcctcgt ctagcctgca gtcatcaaca aacacatgca cacatccata 
    11581 cacacataat ttaaaagaaa gcgaaagtaa cctataatgc taaacaaagc acattctcag 
    11641 tgtagactgc agtggtgatg aaccttgaaa atatattaat agaagccaga cacgtaagtc 
    11701 acaaatggta taactcaatt tatatgaaat tcccagagag tccaaagtta aaaaaaacaa 
    11761 aaaggcagat tattggttgt cagtagctac agagatggga cattgaagat attaataggt 
    11821 atagggttga ggattcatgg cctttccctc ttccatgtta gcatgtctat tgttgttaac 
    11881 cttattcaac tcatgttagg gcaatcatat tgatgagact taatgagtat agcttgacat 
    11941 tcctagaaga cagagtctca catcagactc tctgatcctc tgactcttag aatctttcaa 
    12001 caccctcttc tgaaatgatc tctgagcctt tggttgtgac tgaattatga gaaagaattt 
    12061 tatgttgaat gaattttact tcagcatttt tacaaaactg aagctgggga tggcgtttca 
    12121 tgcgtgcagt ccctaccatt tgcctataag cctggaggag gcagaagtag aattaggctt 
    12181 ttcagggaca tcttcagctg catcatgagc ttgaggacaa cttgagctac ataagattcc 
    12241 catccccacc cccacacaca cacacacatc tagtatgtac ctagtatgca gtcttttcat 
    12301 atgtttaaaa tgtagaaagg tatatgtgaa gtctcccttt tccccacaac ttagtccctc 
    12361 caaagtattg catcagtttc cttgtacatg attttatgca tatataagat acactatttc 
    12421 tctctctctc tctctccttc cctccttccc tccaacctct gggtatgagt tgagggttat 
    12481 gtatgtttca agttgtaata agaattgcca ctcaaacatt tcatcccatt ttaagttaag 
    12541 tttgtctgat tttccacttt agaagggcag ccatgatggc tcatgcctat aatcccatcc 
    12601 tagcacttgt aagcctaata cataagactg gaggattgtc atcagcttga ggctagcctg 
    12661 gtctacctaa tgagttccag ccagccaaga gtagaatata agaccctgtc tccaaaaaat 
    12721 gtaccaaagg ggctggaggg gtaactcagt ggttaagaac agtagctgct cttccagagg 
    12781 acctgggttc aattcccagc acccacatgg cagcttgcaa ctatctgtaa ctgtccagct 
    12841 ttgggatatc cgacaccatt ataccaatta cataaaatga aaaaattgta aaatgtgcta 
    12901 aaatgagtgg ggagggggag catggtgtgg ttctgtgggc ttgggatttt gccatgaatc 
    12961 catgactata tctcagcaat atgtggatta tttctggaaa cctgtggggc atgggtgtat 
    13021 gggaagaggg gagagagaga gagcccgcat ccagccagag ttcctgtgct ctgggcaggc 
    13081 ggacgtggga ggactgctgg acgatttcca cttggccctg ggtgggcatc tggctgtgtg 
    13141 aagccactga ccccactcag cggggggtag ataaggggca tttcccatac caggttctca 
    13201 gactccagca ttccacgctg gatacagcag aagagctgag aacagaatag gggccccagg 
    13261 gagacactct cgtcccagag ataaagaagg cagagggaga gagattccta cgcaggcgag 
    13321 aggccttagt ctgggccttg atgaacagag acggtctatg gttttagagc tttattgtag 
    13381 aaaggcaggg ggaaagagag aaggtagaaa gagagagacc agccatggcc aagaggagag 
    13441 aaggggggaa aggagagaga gaagaaaggc tagagagtaa gaaaggttaa agagacagaa 
    13501 gtaaaagctt agagtaagag agagagagag agcaagaggg agagtaagaa aagtaaaagc 
    13561 ttaagagagc gaggaggggc cagcccctct aatagtgggc ttgttatctt gttgttgcta 
    13621 ggtaactgtg aagcgtctag cctgaaggcc agaagcttgg gacattgtct atgtgactga 
    13681 aagccatgag cctctactgt gggggctgtg gaggtggtaa cttggacagg agccaggggt 
    13741 tccaggagac atgagggaat gccttctgtc ccatgtaggt aggaattacc accaccggct 
    13801 ttcaccgtag ttcactggag actgtttgta catagcccat tgccccacag aaaccaggca 
    13861 ctcactgttt catttgcata agaattagaa ggctttattt tggctgggat tggtgacttg 
    13921 ccccagaaat tccagctttt gggaagccga atcctggaga accaggagtt caaggtcatc 
    13981 ctcaggtacc tcatttgttc aagaccagcc tgtgctacca tgagaccttg tccctactcc 
    14041 cctcaacccc aaaacacgct gttttcattt tataggttgt actaaccatg gaggatggtc 
    14101 atttgccttt ctcttgattt cagaagcgta gcgcgggctt tgcaccatga tctagtgttt 
    14161 ccagataggc ttgcctctcc acagccaggc ttatcctgct tcagtcccat gtttggcaag 
    14221 ggcaacatat gactgaagaa cttgcatagc cttatggtgt atcatgactc ttagccccag 
    14281 tttataactg tctaacagct ggtggctttt gtgaaaaagg aaagcatcat ttgaactttt 
    14341 aacttttcag ctgtttgttt tgactttgca taaatactga gactagctag gaaatgagct 
    14401 tttgtttcat ttgctaaaaa tgattttttt ttcatgaggc aatttcaagc aatttgaatt 
    14461 tagcaatctc tacttgtctg aaaagaagtt attaattaaa aaaaaacaaa caaacaggaa 
    14521 cctattatgc tggctagtca catgaaagaa tccaaatgaa cacagtcaag gctgaatcaa 
    14581 cacatgaaat tattttctaa ttccccaaat tacatttatg ctaattagta tgtagtaaaa 
    14641 cattttaatt gtttccaatg ttaaacatta cagaaataat tttgatgttt tcctaataac 
    14701 ccagcctttg atcatgtttg catttatttg attgttgtca gtttattaaa taattaagta 
    14761 agagcaacaa gataaaatga cttcatgaga gtccttttct gtaacttaga acgtcagtag 
    14821 tcataggaac tgcggtcgtt cccttacctt tcagaagtag gctaaagagt tgaacgcaaa 
    14881 ggtggttttt cgaatcttcc tgatccttaa gcccccacta ggtttatatt ctatatttga 
    14941 tatcggattc gaacccctcg agcttaggat ccggaaccct taatataact aagttattag 
    15001 gtccctcgaa gaggttcact agtactggcc attgcggccg cttcgaggct gcctcgcgcg 
    15061 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
    15121 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
    15181 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 
    15241 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
    15301 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
    15361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
    15421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
    15481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
    15541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
    15601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
    15661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
    15721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
    15781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
    15841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
    15901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
    15961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
    16021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
    16081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
    16141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
    16201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
    16261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
    16321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
    16381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
    16441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
    16501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
    16561 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
    16621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
    16681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
    16741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
    16801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
    16861 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 
    16921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
    16981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
    17041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
    17101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
    17161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
    17221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgg 
//
