LOCUS pEMS1985 6343 bp DNA circular 9-OCT-2012 SOURCE ORGANISM COMMENT http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|638813164| COMMENT VNTDBDATE|639494000| COMMENT LSOWNER| COMMENT VNTNAME|pEMS1985| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pEMS1985" 0 6343 COMMENT (CDBMol 0 0 1 4700 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 1 0 0 8 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 100 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "AhdI" "AscI" "AvrII" "BamHI" "BglI" "BglII" "EcoRI" "FseI" COMMENT "MluI" "NotI" "SmaI") (CStringList "atg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 1 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 6343 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 8 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 60 0 3476 0 5084 0 0 COMMENT "") #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1868 0 0 "") COMMENT #2=(CRSite (CStringList) "AvrII" "cctagg" 2 0 2 198 0 727 0 0 "") COMMENT #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #4=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 7 32 0 456 0 1087 0 COMMENT 2456 0 3506 0 3699 0 4966 0 0 "") COMMENT #5=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2360 0 0 "") COMMENT #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 192 0 0 "") COMMENT #7=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 207 0 0 "") COMMENT #8=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 210 0 0 "") COMMENT #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 2049 0 3093 0 0 "") COMMENT #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 6 32 0 43 0 343 0 2834 0 COMMENT 3492 0 3503 0 0 "") COMMENT #11=(CRSite (CStringList) "atg" "atg" 1 0 95 139 0 153 0 174 0 377 0 414 COMMENT 0 421 0 511 0 543 0 583 0 782 0 982 0 1035 0 1668 0 1708 0 1717 0 COMMENT 1744 0 1751 0 1770 0 2059 0 2108 0 2120 0 2140 0 2146 0 2188 0 2244 COMMENT 0 2267 0 2347 0 2390 0 2407 0 2429 0 2435 0 2503 0 2520 0 2624 0 COMMENT 2635 0 2727 0 2753 0 2792 0 2876 0 2888 0 2953 0 2986 0 3008 0 3022 COMMENT 0 3061 0 3080 0 3109 0 3125 0 3151 0 3165 0 3188 0 3259 0 3280 0 COMMENT 3318 0 3359 0 3380 0 3418 0 3698 0 3705 0 3932 0 4106 0 4186 0 4233 COMMENT 0 4245 0 4266 0 4296 0 4393 0 4491 0 4494 0 4517 0 4588 0 4633 0 COMMENT 4638 0 4655 0 4674 0 4752 0 4762 0 4798 0 4833 0 4845 0 4920 0 5044 COMMENT 0 5097 0 5101 0 5253 0 5720 0 5885 0 5909 0 5973 0 6146 0 6205 0 COMMENT 6249 0 6264 0 6313 0 6319 0 0 "") COMMENT #12=(CRSite (CStringList) "cat" "cat" 1 0 70 114 0 152 0 173 0 376 0 420 COMMENT 0 582 0 612 0 619 0 781 0 888 0 894 0 969 0 973 0 1110 0 1205 0 COMMENT 1582 0 1612 0 1667 0 1681 0 1750 0 2007 0 2058 0 2145 0 2319 0 2346 COMMENT 0 2529 0 2553 0 2577 0 2610 0 2646 0 2952 0 2973 0 2985 0 3015 0 COMMENT 3030 0 3060 0 3108 0 3120 0 3210 0 3249 0 3253 0 3358 0 3379 0 3393 COMMENT 0 3622 0 3728 0 3950 0 4224 0 4244 0 4308 0 4342 0 4427 0 4571 0 COMMENT 4623 0 4637 0 4667 0 4673 0 4751 0 4761 0 4805 0 5024 0 5148 0 5176 COMMENT 0 5205 0 5252 0 5530 0 5972 0 6141 0 6222 0 6318 0 0 "") COMMENT #13=(CRSite (CStringList) "taa" "taa" 1 0 72 137 0 144 0 744 0 764 0 COMMENT 1004 0 1097 0 1510 0 1725 0 1805 0 1901 0 1927 0 3113 0 3206 0 3215 COMMENT 0 3225 0 3234 0 3295 0 3304 0 3341 0 3372 0 3389 0 3396 0 3537 0 COMMENT 3541 0 3546 0 3606 0 3645 0 3731 0 3857 0 4002 0 4069 0 4100 0 4117 COMMENT 0 4128 0 4140 0 4151 0 4218 0 4254 0 4263 0 4275 0 4387 0 4451 0 COMMENT 4505 0 4645 0 4669 0 4682 0 4732 0 4765 0 4870 0 4909 0 4931 0 4988 COMMENT 0 5048 0 5144 0 5154 0 5197 0 5211 0 5216 0 5246 0 5268 0 5350 0 COMMENT 5437 0 5545 0 5579 0 5624 0 5770 0 6008 0 6144 0 6203 0 6288 0 6335 COMMENT 0 6339 0 0 "") COMMENT #14=(CRSite (CStringList) "tta" "tta" 1 0 81 136 0 143 0 160 0 743 0 747 COMMENT 0 767 0 1208 0 1426 0 1700 0 1715 0 1724 0 1804 0 1913 0 1926 0 COMMENT 2000 0 2708 0 3170 0 3199 0 3212 0 3233 0 3257 0 3294 0 3388 0 3395 COMMENT 0 3536 0 3540 0 3565 0 3596 0 3605 0 3730 0 3752 0 3874 0 3887 0 COMMENT 3895 0 3924 0 4001 0 4066 0 4099 0 4116 0 4127 0 4139 0 4150 0 4157 COMMENT 0 4164 0 4209 0 4324 0 4424 0 4504 0 4530 0 4627 0 4653 0 4698 0 COMMENT 4869 0 4885 0 4908 0 4978 0 5068 0 5143 0 5171 0 5186 0 5196 0 5210 COMMENT 0 5215 0 5267 0 5493 0 5553 0 5590 0 5616 0 5834 0 5937 0 5988 0 COMMENT 6016 0 6143 0 6202 0 6212 0 6224 0 6240 0 6247 0 6323 0 6334 0 6338 COMMENT 0 0 "") COMMENT #15=(CRSite (CStringList) "tga" "tga" 1 0 133 21 0 77 0 140 0 223 0 336 COMMENT 0 411 0 415 0 422 0 541 0 544 0 790 0 798 0 849 0 874 0 911 0 956 0 COMMENT 978 0 983 0 1071 0 1175 0 1285 0 1290 0 1317 0 1367 0 1405 0 1579 0 COMMENT 1591 0 1709 0 1752 0 1853 0 1885 0 1981 0 2004 0 2072 0 2118 0 2121 COMMENT 0 2138 0 2172 0 2231 0 2256 0 2262 0 2270 0 2312 0 2382 0 2405 0 COMMENT 2408 0 2469 0 2478 0 2484 0 2501 0 2514 0 2603 0 2639 0 2751 0 2754 COMMENT 0 2853 0 2870 0 2889 0 2979 0 3011 0 3020 0 3023 0 3048 0 3065 0 COMMENT 3088 0 3110 0 3123 0 3126 0 3157 0 3177 0 3186 0 3416 0 3459 0 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5204 0 COMMENT 5251 0 5295 0 5314 0 5408 0 5446 0 5506 0 5604 0 5892 0 5969 0 6075 COMMENT 0 6140 0 6217 0 6221 0 6296 0 0 "") COMMENT #17=(CRSite (CStringList) "tag" "tag" 1 0 55 120 0 133 0 169 0 180 0 199 COMMENT 0 316 0 529 0 625 0 728 0 748 0 768 0 896 0 959 0 1170 0 1178 0 COMMENT 1192 0 1209 0 1348 0 1939 0 1984 0 3017 0 3147 0 3324 0 3353 0 3368 COMMENT 0 3376 0 3413 0 3648 0 3720 0 3788 0 3875 0 3888 0 3925 0 3942 0 COMMENT 3961 0 4005 0 4165 0 4840 0 4891 0 4912 0 5065 0 5111 0 5128 0 5187 COMMENT 0 5226 0 5305 0 5479 0 5484 0 5490 0 5494 0 5518 0 5613 0 5837 0 COMMENT 6213 0 6225 0 0 "") COMMENT #18=(CRSite (CStringList) "cta" "cta" 1 0 59 119 0 156 0 164 0 179 0 198 COMMENT 0 315 0 528 0 595 0 700 0 727 0 756 0 763 0 1169 0 1347 0 1509 0 COMMENT 1518 0 1526 0 1659 0 1991 0 2277 0 2559 0 2766 0 2874 0 3027 0 3146 COMMENT 0 3191 0 3312 0 3352 0 3363 0 3399 0 3412 0 3545 0 3770 0 3787 0 COMMENT 3856 0 4043 0 4053 0 4092 0 4201 0 4217 0 4515 0 4577 0 4731 0 4866 COMMENT 0 4881 0 4890 0 5072 0 5095 0 5225 0 5382 0 5415 0 5478 0 5527 0 COMMENT 5544 0 5687 0 5706 0 5718 0 5907 0 6311 0 0 "")) COMMENT (CObList COMMENT #19=(CFSignal (CObList) "Amp-R" 4 0 0 4296 5153 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5106..5963") COMMENT #20=(CFSignal (CObList) "Origin" 21 0 0 5327 5915 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6137..6725") COMMENT #21=(CFSignal (CObList) "additional AAV sequences" 21 0 0 3358 3403 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4168..4213") COMMENT #22=(CFSignal (CObList) "5' ITR" 34 0 0 1 168 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1..168") COMMENT #23=(CFSignal (CObList) "3' ITR" 34 0 1 3366 3533 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4176..4343)") COMMENT #24=(CFSignal (CObList) "iCre" 4 0 0 2059 3090 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2869..3900") COMMENT #25=(CFSignal (CObList) "MCS 5' end" 21 0 0 197 208 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "197..208") COMMENT #26=(CFSignal (CObList) "MSC 3' end" 21 0 0 1866 1873 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2676..2683") COMMENT #27=(CFSignal (CObList) "SV40" 25 0 0 3105 3325 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3915..4135") COMMENT #28=(CFSignal (CObList) "Intron cassette" 15 0 0 1874 2046 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2684..2856") COMMENT #29=(CFSignal (CObList) "Ple155 (PCP2-B)" 21 0 0 214 1865 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "214..1865") COMMENT #30=(CFSignal (CObList) "Prom1 region" 21 0 0 880 1865 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "880..1865") COMMENT #31=(CFSignal (CObList) "Prom2 region" 21 0 1 214 879 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(214..879)") COMMENT #32=(CFSignal (CObList) "oEMS5913" 27 0 1 2075 2095 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #33=(CFSignal (CObList) "oEMS5932" 27 0 1 422 442 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) COMMENT (CObList COMMENT #34=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 COMMENT 56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) COMMENT (CPrimer 0 "gattgggaaggagactcatgtctca" 63 415 25 1 100 100 100 COMMENT 57.3753 48 -175 -450.3 -38.9631 -11.4724) 171 101 439 339 81.9796 COMMENT 58.7021 59.4314) COMMENT #35=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 COMMENT 56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) COMMENT (CPrimer 0 "ggattgggaaggagactcatgtct" 63 417 24 1 100 100 100 COMMENT 56.4291 50 -174.6 -450.5 -38.5034 -11.4724) 171 101 440 340 COMMENT 82.0353 58.8235 59.4534) COMMENT #36=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 COMMENT 56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) COMMENT (CPrimer 0 "gggattgggaaggagactcatgt" 63 419 23 1 100 100 100 COMMENT 57.0503 52.1739 -172.2 -442.8 -38.3992 -11.2394) 171 101 441 341 COMMENT 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100 100 100 56.1144 COMMENT 50 -166.9 -429 -37.2137 -13.3672) 171 101 497 397 83.0918 60.7053 COMMENT 60.0986) COMMENT #43=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "gtggccaactccatcactaggg" 63 103 22 0 100 100 100 57.1266 COMMENT 59.0909 -167.1 -428.2 -37.6522 -13.5925) COMMENT (CPrimer 0 "ggattgggaaggagactcatgtct" 63 417 24 1 100 100 100 COMMENT 56.4291 50 -174.6 -450.5 -38.5034 -11.4724) 171 103 440 338 COMMENT 82.0449 58.8757 59.4602)) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #44=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #45=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEMS1985" 2) 5 "" COMMENT 0 4) COMMENT #46=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pEMS1985'" 1) COMMENT #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. 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#32# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #114=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2075 End: 2095 (Complementary)" COMMENT 1))) "oEMS5913"))) "Primer (2 total)") COMMENT #115=(CFolderPar COMMENT (CGroupPar (CParagraph 34 (7 34 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #116=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 168 " 1) COMMENT #118=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #119=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..168" 1))) "5' ITR") COMMENT #120=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3366 (3 #23# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3366 End: 3533 (Complementary)" COMMENT 1) COMMENT #122=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #123=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4176..4343)" 1))) COMMENT "3' ITR"))) "Repeat Region (2 total)"))) COMMENT "Feature Map") COMMENT #124=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80550584 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #126=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 60 " 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 3476 " 1) COMMENT #129=(CLinePar COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191) COMMENT " N3: 5084 " 1))))) "AhdI: 3 sites") COMMENT 6 5 "GACNNNNNGTC" "CTGNNNNNCAG") COMMENT #130=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80550520 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #131=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #132=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 1868 " 1))))) "AscI: 1 site") COMMENT 2 6 "GGCGCGCC" "CCGCGCGG") COMMENT #133=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80550456 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #134=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #135=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 198 " 1) COMMENT #136=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 727 " 1))))) "AvrII: 2 sites") COMMENT 1 5 "CCTAGG" "GGATCC") COMMENT #137=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80551672 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #138=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80551064 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #139=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #140=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 32 " 1) COMMENT #141=(CLinePar COMMENT (CParagraph 0 (1 #4# 2) 1 2 2 0 191) COMMENT " N2: 456 " 1) COMMENT #142=(CLinePar COMMENT (CParagraph 0 (1 #4# 3) 1 2 2 0 191) COMMENT " N3: 1087 " 1) COMMENT #143=(CLinePar COMMENT (CParagraph 0 (1 #4# 4) 1 2 2 0 191) COMMENT " N4: 2456 " 1) COMMENT #144=(CLinePar COMMENT (CParagraph 0 (1 #4# 5) 1 2 2 0 191) COMMENT " N5: 3506 " 1) COMMENT #145=(CLinePar COMMENT (CParagraph 0 (1 #4# 6) 1 2 2 0 191) COMMENT " N6: 3699 " 1) COMMENT #146=(CLinePar COMMENT (CParagraph 0 (1 #4# 7) 1 2 2 0 191) COMMENT " N7: 4966 " 1))))) "BglI: 7 sites") COMMENT 7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") COMMENT #147=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80551000 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #148=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #149=(CLinePar COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191) COMMENT " N1: 2360 " 1))))) "BglII: 1 site") COMMENT 1 5 "AGATCT" "TCTAGA") COMMENT #150=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80549080 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #151=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #152=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 192 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #153=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80550392 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #154=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #155=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 207 " 1))))) "FseI: 1 site") 6 COMMENT 2 "GGCCGGCC" "CCGGCCGG") COMMENT #156=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 80550328 (8 0) 1 1 1 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0 191) COMMENT " N1: 32 " 1) COMMENT #166=(CLinePar COMMENT (CParagraph 0 (1 #10# 2) 1 2 2 0 191) COMMENT " N2: 43 " 1) COMMENT #167=(CLinePar COMMENT (CParagraph 0 (1 #10# 3) 1 2 2 0 191) COMMENT " N3: 343 " 1) COMMENT #168=(CLinePar COMMENT (CParagraph 0 (1 #10# 4) 1 2 2 0 191) COMMENT " N4: 2834 " 1) COMMENT #169=(CLinePar COMMENT (CParagraph 0 (1 #10# 5) 1 2 2 0 191) COMMENT " N5: 3492 " 1) COMMENT #170=(CLinePar COMMENT (CParagraph 0 (1 #10# 6) 1 2 2 0 191) COMMENT " N6: 3503 " 1))))) "SmaI: 6 sites") COMMENT 3 3 "CCCGGG" "GGGCCC"))) COMMENT "Restriction/Methylation Map: (no more than 8 sites)") COMMENT #171=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #172=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 1 0 178) COMMENT (CObjectList COMMENT #173=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 2 0 178) COMMENT (CObjectList)) "Reading Frame 1 (0 ORFs)") COMMENT #174=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 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COMMENT "#1: Product of length 339 (rating: 171)")) COMMENT #199=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #200=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "ggattgggaaggagactcatgtct" 0 101 440 340 0 COMMENT 0) 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #201=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 440" COMMENT 1) COMMENT #202=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.0 C TaOpt: 59.5 C GC: 58.8" 1) COMMENT #203=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #204=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #205=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #206=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #207=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #208=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #209=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #210=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #211=(COligo "" COMMENT "GGATTGGGAAGGAGACTCATGTCT" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GGATTGGGAAGGAGACTCATGTCT" 1)) COMMENT #212=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #213=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 24 Tm: 56.4 C GC: 50.0" 1) COMMENT #214=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -174.6 kcal/mol dS: -450.5 cal/mol dG: -38.5 kcal/mol" COMMENT 1) COMMENT #215=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.1" 1) COMMENT #216=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 6.5" 1))) COMMENT "#2: Product of length 340 (rating: 171)")) COMMENT #217=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #218=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "gggattgggaaggagactcatgt" 0 101 441 341 0 COMMENT 0) 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #219=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 441" COMMENT 1) COMMENT #220=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.1 C TaOpt: 59.5 C GC: 58.9" 1) COMMENT #221=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #222=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #223=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region 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#232=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -172.2 kcal/mol dS: -442.8 cal/mol dG: -38.4 kcal/mol" COMMENT 1) COMMENT #233=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.6" 1) COMMENT #234=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 4.3" 1))) COMMENT "#3: Product of length 341 (rating: 171)")) COMMENT #235=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #236=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "gggattgggaaggagactcatg" 0 101 441 341 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #237=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 441" COMMENT 1) COMMENT #238=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.1 C TaOpt: 59.4 C GC: 58.9" 1) COMMENT #239=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #240=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #241=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #242=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #243=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #244=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #245=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #246=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #247=(COligo "" "GGGATTGGGAAGGAGACTCATG" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GGGATTGGGAAGGAGACTCATG" 1)) COMMENT #248=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #249=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 56.2 C GC: 54.5" 1) COMMENT #250=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -165.7 kcal/mol dS: -425.5 cal/mol dG: -37.1 kcal/mol" COMMENT 1) COMMENT #251=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.3" 1) COMMENT #252=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 2.0" 1))) COMMENT "#4: Product of length 341 (rating: 171)")) COMMENT #253=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #254=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "cgggattgggaaggagactcat" 0 101 442 342 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #255=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 442" COMMENT 1) COMMENT #256=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.1 C TaOpt: 59.5 C GC: 59.1" 1) COMMENT #257=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #258=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #259=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #260=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #261=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #262=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #263=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #264=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #265=(COligo "" "CGGGATTGGGAAGGAGACTCAT" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " CGGGATTGGGAAGGAGACTCAT" 1)) COMMENT #266=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #267=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 58.0 C GC: 54.5" 1) COMMENT #268=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -171.8 kcal/mol dS: -440.4 cal/mol dG: -38.7 kcal/mol" COMMENT 1) COMMENT #269=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 1.5" 1) COMMENT #270=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 2.0" 1))) COMMENT "#5: Product of length 342 (rating: 171)")) COMMENT #271=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #272=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "cgggattgggaaggagactca" 0 101 442 342 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #273=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 442" COMMENT 1) COMMENT #274=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.1 C TaOpt: 59.5 C GC: 59.1" 1) COMMENT #275=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #276=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #277=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #278=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #279=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #280=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #281=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #282=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #283=(COligo "" "CGGGATTGGGAAGGAGACTCA" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " CGGGATTGGGAAGGAGACTCA" 1)) COMMENT #284=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #285=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 21 Tm: 57.6 C GC: 57.1" 1) COMMENT #286=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -163.2 kcal/mol dS: -416.5 cal/mol dG: -37.2 kcal/mol" COMMENT 1) COMMENT #287=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 1.1" 1) COMMENT #288=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 0.6" 1))) COMMENT "#6: Product of length 342 (rating: 171)")) COMMENT #289=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #290=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "ccgggattgggaaggagact" 0 101 443 343 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #291=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 443" COMMENT 1) COMMENT #292=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.2 C TaOpt: 59.6 C GC: 59.2" 1) COMMENT #293=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #294=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #295=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #296=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #297=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #298=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #299=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #300=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #301=(COligo "" "CCGGGATTGGGAAGGAGACT" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " CCGGGATTGGGAAGGAGACT" 1)) COMMENT #302=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #303=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 56.6 C GC: 60.0" 1) COMMENT #304=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -162.8 kcal/mol dS: -416.7 cal/mol dG: -36.8 kcal/mol" COMMENT 1) COMMENT #305=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.1" 1) COMMENT #306=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 3.5" 1))) COMMENT "#7: Product of length 343 (rating: 171)")) COMMENT #307=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #308=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "tgaagcggtaccacgtgaagtg" 0 101 496 396 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #309=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 496" COMMENT 1) COMMENT #310=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 83.2 C TaOpt: 60.3 C GC: 60.9" 1) COMMENT #311=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #312=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #313=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #314=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #315=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #316=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #317=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #318=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #319=(COligo "" "TGAAGCGGTACCACGTGAAGTG" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " TGAAGCGGTACCACGTGAAGTG" 1)) COMMENT #320=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #321=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 57.4 C GC: 54.5" 1) COMMENT #322=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -163.6 kcal/mol dS: -417.9 cal/mol dG: -37.2 kcal/mol" COMMENT 1) COMMENT #323=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.9" 1) COMMENT #324=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 2.0" 1))) COMMENT "#8: Product of length 396 (rating: 171)")) COMMENT #325=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #326=(CPCRData (CStringList) (CStringList) COMMENT "gagtggccaactccatcactagg" 0 COMMENT "ttgaagcggtaccacgtgaagt" 0 101 497 397 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #327=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 101 to 497" COMMENT 1) COMMENT #328=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 83.1 C TaOpt: 60.1 C GC: 60.7" 1) COMMENT #329=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #330=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #331=(COligo "" COMMENT "GAGTGGCCAACTCCATCACTAGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GAGTGGCCAACTCCATCACTAGG" 1)) COMMENT #332=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #333=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 23 Tm: 56.5 C GC: 56.5" 1) COMMENT #334=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -169.5 kcal/mol dS: -435.9 cal/mol dG: -37.8 kcal/mol" COMMENT 1) COMMENT #335=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #336=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #337=(COligo "" "TTGAAGCGGTACCACGTGAAGT" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " TTGAAGCGGTACCACGTGAAGT" 1)) COMMENT #338=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #339=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 56.1 C GC: 50.0" 1) COMMENT #340=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -166.9 kcal/mol dS: -429.0 cal/mol dG: -37.2 kcal/mol" COMMENT 1) COMMENT #341=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.4" 1) COMMENT #342=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 6.5" 1))) COMMENT "#9: Product of length 397 (rating: 171)")) COMMENT #343=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #344=(CPCRData (CStringList) (CStringList) COMMENT "gtggccaactccatcactaggg" 0 COMMENT "ggattgggaaggagactcatgtct" 0 103 440 338 0 COMMENT 0) 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #345=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 103 to 440" COMMENT 1) COMMENT #346=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 82.0 C TaOpt: 59.5 C GC: 58.9" 1) COMMENT #347=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #348=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #349=(COligo "" "GTGGCCAACTCCATCACTAGGG" COMMENT "Sense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GTGGCCAACTCCATCACTAGGG" 1)) COMMENT #350=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #351=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 57.1 C GC: 59.1" 1) COMMENT #352=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -167.1 kcal/mol dS: -428.2 cal/mol dG: -37.7 kcal/mol" COMMENT 1) COMMENT #353=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #354=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #355=(COligo "" COMMENT "GGATTGGGAAGGAGACTCATGTCT" COMMENT "Antisense primer for PCR amplification of a region of pEMS1985" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " GGATTGGGAAGGAGACTCATGTCT" 1)) COMMENT #356=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #357=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 24 Tm: 56.4 C GC: 50.0" 1) COMMENT #358=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -174.6 kcal/mol dS: -450.5 cal/mol dG: -38.5 kcal/mol" COMMENT 1) COMMENT #359=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.7" 1) COMMENT #360=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 9.1" 1))) COMMENT "#10: Product of length 338 (rating: 171)")))) COMMENT "PCR Analysis")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #361=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #362=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #363=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #364=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #365=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #366=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #367=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #368=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #369=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #370=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #371=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #372=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #373=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #374=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #375=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #376=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #377=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #378=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #379=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #380=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #381=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #382=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #383=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #384=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #385=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #386=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #387=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #388=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #389=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #390=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #391=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #392=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #393=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #394=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #395=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #396=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #397=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #398=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #399=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #400=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 60.0567 60.0567 58.5 58.5 0.1 COMMENT -34817) 1 1 0 1 1 COMMENT (mapper: 17.5439 -22.5492 60.0567 60.0567 0.01 10 -1 6343 6343 1 0 0) COMMENT #401=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -604 100) COMMENT (CObjectList COMMENT #402=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1277 100) COMMENT (CObjectList COMMENT #403=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #367# 82408244 0) COMMENT (LOGPEN 0 2 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27517 6.27317 1 0.0214037) 0.0988993) COMMENT #404=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #364# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 87 33 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pEMS1985" "@N" 1 0 0.871165 0 COMMENT -10 5.82783 1.46528 Nil) COMMENT #405=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #363# 673198112 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "6343 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.06377 0.932452 Nil)) (CObjectList)) COMMENT #406=(CGroupWidget (CWidget 13 (12 0) 1 2 0 0 Nil -1696 100) COMMENT (CObjectList) (CObjectList)) COMMENT #407=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1800 100) COMMENT (CObjectList COMMENT #408=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -2533 100) COMMENT (CObjectList COMMENT #409=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #365# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 1.1769 2.02546 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #410=(CLabel COMMENT (CWidget 0 (0 0) 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(CWidget 12 (0 0) 1 2 0 0 Nil -2219 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #507=(AnlzFontBackColorItem 2074 2094 33619967) COMMENT #508=(AnlzFontBackColorItem 421 441 33619967))) (CObList) 34078861 COMMENT (CStringList) 1097859071 327712 (CObList))) FEATURES Location/Qualifiers CDS 4296..5153 /vntifkey="4" /label=Amp-R misc_feature 5327..5915 /vntifkey="21" /label=Origin misc_feature 3358..3403 /vntifkey="21" /label=additional\AAV\sequences repeat_region 1..168 /vntifkey="34" /label=5'\ITR repeat_region complement(3366..3533) /vntifkey="34" /label=3'\ITR CDS 2059..3090 /vntifkey="4" /label=iCre misc_feature 197..208 /vntifkey="21" /label=MCS\5'\end misc_feature 1866..1873 /vntifkey="21" /label=MSC\3'\end polyA_signal 3105..3325 /vntifkey="25" /label=SV40 intron 1874..2046 /vntifkey="15" /label=Intron\cassette misc_feature 214..1865 /vntifkey="21" /label=Ple155\(PCP2-B) misc_feature 880..1865 /vntifkey="21" /label=Prom1\region misc_feature complement(214..879) /vntifkey="21" /label=Prom2\region primer complement(2075..2095) /vntifkey="27" /label=oEMS5913 primer complement(422..442) /vntifkey="27" /label=oEMS5932 BASE COUNT 1442 a 1684 c 1749 g 1468 t ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccac gcgtcagcag attgaagaag ccctcctggt 241 ctggggagcc cgcctgggga cagactcgct cagtctggtt ggcccttcag cttgggggcc 301 cctccccaaa cttcctagcc agcttgctca caccctgacc ccggggcctg ccgtccccac 361 ttcctcagct ctcaccatgg tccccgccga tcctctctgc agagctgttc tgaatgagac 421 atgagtctcc ttcccaatcc cggcccccgc cccaggggcc ctggccagca gtgccacttc 481 acgtggtacc gcttcaaggg acaggctccg atgcgtgtcc cgccgtccta gattggggcc 541 tgatgagtgt ggcctgggag ctgggacacg aatcagggaa acatggccca ggagctaccc 601 ccaggtccca gcatctccca tcaatagggg tccacacgga gagccctgcc ctctgccctg 661 gggcctggca ctcagacccc caagcccacc agcccctttc tacagccaca actgggtcag 721 ggggtcctag gagactcact cgttaattag gtgccctaca aactaattag tcttgtcaat 781 catgggctct gagaccttga gctgggggtg gggtgggggc agggccctct cacctcggca 841 caggggcctg agccttcctc cgtcttctcc tcctgatccg gacacttcat tggcatagag 901 ggagagagtg tgaacttggc cctttgtgga acagaggagg ctcgggcaga ggtggtgata 961 gtgcagccca ttcattctga gatgaaactt ccactggttt ccgtaaagac gtcttgggga 1021 gggaagggaa ggggatgggg acctcccagt ggtatcccct gcttgggcac tgagggaaag 1081 ccacagtggc tcggggtaaa aggcagggac atcctctccc cgcctgcctc tgtccccagg 1141 gagtctcgcc tcctgttccc acctggggct agggtgatag aggagaggag atagctcaac 1201 ctggcattta ggtggtgtgg gaacaggaga ccccagactt tcttgttttg gggtctgggg 1261 caggcaacca ggctccaggg acagtgagtt gaaggaaggg tggctgggag accccttgac 1321 ttgctgccaa ggagacagag ctggagctag ggtggcgggt ggtgtctgag gcaggtgcag 1381 agagggaggg agggaagggg cctttgactc caacctcctt tttctttacc gactgcaggt 1441 ggcagctgcc cttccaggag ccagtggggg aacctgggtg gctgggtggg gacacctgca 1501 agtcctccct aagccagcta ccaccctaca ctgttggcct cccttctcca actgtgggga 1561 cgctgctcag gccttttgtg acatcacacc tgagagtccc tggggtccag tcattgctgc 1621 tgggcacagc gaggtccaag ctcaggtcgc cctgccccct acccaccatg ccagatccag 1681 catcgttgtg ggcaaacaat tatctggatg atctttatgg ggcttaagct tgggtgggag 1741 cagatggggc atgagctggg gatttgggga tggggggaat ccacaccccc acgtcctgga 1801 cgtttaaaag gccctctctg gcactgggcc ggggcagagg ccagcagaaa agtgactgga 1861 gtccaggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga 1921 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc 1981 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac 2041 tcccaggcgg ccgccaccat gtccaacctg ctgactgtgc accaaaacct gcctgccctc 2101 cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg 2161 caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc 2221 tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac 2281 ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag 2341 ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc 2401 ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc 2461 ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga 2521 tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt 2581 gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg 2641 atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc 2701 ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc 2761 aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc 2821 caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt 2881 gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt 2941 gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc 3001 tggaccaatg tgaacatagt gatgaactac atcagaaacc tggactctga gactggggcc 3061 atggtgaggc tgctcgagga tggggactga gcggccgcga tcgcagacat gataagatac 3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 3241 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc 3301 aagtaaaacc tctacaaatg tggtaggtcg aaattcccga taaggatctt cctagagcat 3361 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg 3421 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 3481 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat 3541 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 3601 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 3661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 3721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3781 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3841 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 3901 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 3961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 4141 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 4201 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 4261 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 4321 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 4381 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 4441 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 4501 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 4561 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 4621 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 4681 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 4741 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 4801 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 4861 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 4921 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 4981 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 5041 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 5101 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 5161 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 5221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 5281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 5341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 5401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 5461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 5521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 5581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 5641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 5701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 5761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 5821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 5881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 6001 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 6061 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 6121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 6181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 6241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 6301 caggaaacag ctatgaccat gattacgcca gatttaatta agg //