LOCUS       pCA-G-intron-G       5876 bp    DNA     circular SYN 19-MAR-1980
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
ORGANISM
COMMENT
FEATURES             Location/Qualifiers
     promoter        721..2445
                     /vntifkey="30"
                     /label=CA\promoter
                     /note="CA promoter"
     RBS             2477..2484
                     /vntifkey="32"
                     /label=Kozak
                     /note="Kozak"
     exon            2485..2758
                     /vntifkey="61"
                     /label=GFP
                     /note="GFP"
     intron          2759..2928
                     /vntifkey="15"
                     /label=Intron
                     /note="Intron"
     misc_feature    2776..2809
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     polyA_signal    3385..3640
                     /vntifkey="25"
                     /label=SV40\poly\A
                     /note="SV40 poly A"
     CDS             complement(4891..5751)
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR"
     exon            2929..3380
                     /vntifkey="61"
                     /label=GFP
BASE COUNT     1300 a      1576 c      1678 g      1322 t 
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gaggtcgacg gtatcgataa gcttccctta attaaccgtt taaacaattc 
      721 tgcaggaatc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      781 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      841 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      901 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      961 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
     1021 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
     1081 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc 
     1141 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg 
     1201 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg 
     1261 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta 
     1321 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcggggagtc 
     1381 gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg 
     1441 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
     1501 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct 
     1561 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg 
     1621 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg 
     1681 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg 
     1741 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt 
     1801 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc 
     1861 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc 
     1921 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc 
     1981 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg 
     2041 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg 
     2101 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     2161 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 
     2221 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 
     2281 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 
     2341 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 
     2401 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgattt gataccgcgg 
     2461 gcccgggatc cgatatccgc caccatggtg agcaagggcg aggagctgtt caccggggtg 
     2521 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
     2581 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 
     2641 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 
     2701 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggt 
     2761 tggtatcaaa gatctataac ttcgtatagc atacattata cgaagttatg gttacaagac 
     2821 aggtttaagg agaccaatag aaactgggca tgtggagaca gagaagactc ttgggtttct 
     2881 gataggcact gactctctct gcctattggt ctattttccc acccttaggg tacgtccagg 
     2941 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     3001 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     3061 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     3121 acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggcg 
     3181 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     3241 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     3301 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     3361 agctttacaa ggatatccct gaattctaga taactgatca taatcagcca taccacattt 
     3421 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 
     3481 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 
     3541 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     3601 tccaaactca tcaatgtatc ttatttaaat tgggcgcgcc caactagttc tagagcggcc 
     3661 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc 
     3721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     3781 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     3841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     3901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     3961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     4021 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     4081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     4141 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     4201 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     4261 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     4321 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     4381 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     4441 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     4501 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     4561 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     4621 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     4681 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     4741 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     4801 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     4861 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     4921 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     4981 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     5041 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     5101 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     5161 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     5221 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     5281 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     5341 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     5401 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     5461 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     5521 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     5581 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     5641 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     5701 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     5761 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     5821 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgc 
//
