LOCUS       pEMS1989        7005 bp    DNA     circular     10-JUL-2012
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|631296664|
COMMENT     VNTDBDATE|631296664|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1989|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            4165 0 0 "") 
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COMMENT        #2=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 198 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 207 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 210 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1938 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 2119 0 3163 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 2 3172 0 3765 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3144 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 3 168 0 572 0 4029 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 3907 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 592 0 3130 0 0 "")) 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "4760..4805") 
COMMENT        #16=(CFSignal (CObList) "5' ITR" 34 0 0 1 168 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "3' ITR" 34 0 1 4028 4195 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4768..4935)") 
COMMENT        #18=(CFSignal (CObList) "MCS 5' end" 21 0 0 197 208 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "197..208") 
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COMMENT             (CStringList) 1 1 1 1 "2684..2856") 
COMMENT        #20=(CFSignal (CObList) "iCre" 4 0 0 2129 3160 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: User" 1) 
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COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7005 bp" 1) 
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FEATURES             Location/Qualifiers
     CDS             4958..5815
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    5989..6577
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4020..4065
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4028..4195)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          1944..2116
                     /vntifkey="15"
                     /label=Intron
     CDS             2129..3160
                     /vntifkey="4"
                     /label=iCre
     misc_feature    3175..3761
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    209..1943
                     /vntifkey="21"
                     /label=CAGGS
BASE COUNT     1442 a      1886 c      2017 g      1660 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgttgacat tgattattga ctagttatta 
      241 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 
      301 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 
      361 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 
      421 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 
      481 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 
      541 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg 
      601 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 
      661 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc 
      721 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg 
      781 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg 
      841 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt 
      901 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 
      961 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 
     1021 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg 
     1081 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 
     1141 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 
     1201 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga 
     1261 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc 
     1321 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     1381 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     1441 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     1501 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 
     1561 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag 
     1621 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg 
     1681 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 
     1741 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag 
     1801 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 
     1861 tgccttcttc tttttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca 
     1921 ttttggcaaa gaattggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa 
     1981 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 
     2041 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 
     2101 aggtgtccac tcccaggcgg ccgccaccat gtccaacctg ctgactgtgc accaaaacct 
     2161 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt 
     2221 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc 
     2281 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt 
     2341 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca 
     2401 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa 
     2461 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc 
     2521 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa 
     2581 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct 
     2641 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg 
     2701 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa 
     2761 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc 
     2821 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc 
     2881 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct 
     2941 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc 
     3001 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca 
     3061 ggctggtggc tggaccaatg tgaacatagt gatgaactac atcagaaacc tggactctga 
     3121 gactggggcc atggtgaggc tgctcgagga tggggactga gcggccgcga tcgctcaacc 
     3181 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     3241 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     3301 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     3361 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     3421 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     3481 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     3541 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 
     3601 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     3661 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     3721 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgatcgcaga catgataaga 
     3781 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 
     3841 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 
     3901 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa 
     3961 agcaagtaaa acctctacaa atgtggtagt cgaaattccc gataaggatc ttcctagagc 
     4021 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga 
     4081 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 
     4141 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta 
     4201 attaacctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     4261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     4321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 
     4381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     4441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     4501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     4561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     4621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     4681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     4741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     4801 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 
     4861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     4921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     4981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     5041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     5101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     5161 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     5221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     5281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     5341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     5401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     5461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     5521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     5581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     5641 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     5701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     5761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     5821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     5881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     5941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     6001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     6061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     6121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     6181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     6241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     6301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     6361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     6421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     6481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     6541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     6601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     6661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     6721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
     6781 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
     6841 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
     6901 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
     6961 cacaggaaac agctatgacc atgattacgc cagatttaat taagg 
//
