LOCUS bacPuroR-bacNeoR 5447 bp DNA circular SYN 01/03/2005 DEFINITION pPD49_78 complete plasmid sequence KEYWORDS . SOURCE pPD49_78 (unknown) ORGANISM pPD49_78 other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1447 at www.addgene.org FEATURES Location/Qualifiers Region 23..244 /label=5'flank CDS 245..844 /note="originates from bacPuro-pcr_HindIII_NheI" /label=bacPuroR Region 845..997 /label=3'flank Region 1008..1229 /label=5'flank CDS 1230..2024 /note="originates from bacNeo-pcr_SalI_KpnI" /label=bacNeoR Region 2025..2181 /label=3'flank 3'_UTR 2202..2961 /label=unc54_3pUTR Region 3210..3430 /label=5pflank Promoter 3361..3389 /label="Amp_promoter" CDS 3431..4291 /gene="Ampicillin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /label="Ampicillin" Region 4292..4443 /label=3pflank Replication_ori 4446..5065 /label="pBR322_origin" Misc._feature 5281..5307 /label="lacI.C" Promoter 5374..5403 /label="lac_promoter" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT 61 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 121 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 181 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 241 GAGTatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc 301 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 361 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 421 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 481 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 541 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 601 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 661 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 721 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 781 cgccgacgtc gagGtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 841 ctgaCTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 901 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 961 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAGCTA GCGTCGACGA GAAAATACCG 1021 CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 1081 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 1141 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 1201 TGCTTCAATA ATATTGAAAA AGGAAGAGTa tgattgaaca agatggattg cacgcaggtt 1261 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 1321 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 1381 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 1441 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 1501 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 1561 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 1621 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 1681 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 1741 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 1801 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 1861 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 1921 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 1981 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaCTGTCA GACCAAGTTT 2041 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 2101 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 2161 CGTCAGACCC CGTAGAggtc GGTACCATGG TATTGATATC TGAGCTCCGC ATCGGCCGCT 2221 GTCATCAGAT CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC 2281 ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAC 2341 TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT GAAATTGTGT 2401 AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC TCCCTCCCCC 2461 CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT CTTATGTTTT 2521 TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA ATACCTTATC 2581 ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT GGGCCTCTCA 2641 TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT TTTTCAATCA 2701 AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC CCTATTGTTT 2761 GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT GAGCACGATG 2821 CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT 2881 AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA TACATTCCCA 2941 ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT 3001 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 3061 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG 3121 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 3181 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG 3241 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC 3301 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 3361 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 3421 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT 3481 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 3541 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG 3601 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 3661 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 3721 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT 3781 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 3841 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT 3901 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 3961 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 4021 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC 4081 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 4141 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT 4201 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 4261 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT 4321 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 4381 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 4441 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 4501 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 4561 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 4621 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 4681 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 4741 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 4801 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA 4861 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 4921 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 4981 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 5041 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 5101 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 5161 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 5221 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 5281 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 5341 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 5401 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT //