LOCUS JN579121 7213 bp DNA circular SYN 11-APR-2012 DEFINITION Expression vector pDawn, complete sequence. ACCESSION JN579121 VERSION JN579121.1 GI:383460062 KEYWORDS . SOURCE Expression vector pDawn ORGANISM Expression vector pDawn other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7213) AUTHORS Ohlendorf,R., Vidavski,R.R., Eldar,A., Moffat,K. and Moglich,A. TITLE From dusk till dawn: one-plasmid systems for light-regulated gene expression JOURNAL J. Mol. Biol. 416 (4), 534-542 (2012) PUBMED 22245580 REFERENCE 2 (bases 1 to 7213) AUTHORS Ohlendorf,R., Moffat,K. and Moeglich,A. TITLE Direct Submission JOURNAL Submitted (15-AUG-2011) Biophysikalische Chemie, Humboldt Universitaet zu Berlin, Invalidenstrasse 42, Berlin 10115, Germany FEATURES Location/Qualifiers source 1..7213 /organism="Expression vector pDawn" /mol_type="other DNA" /db_xref="taxon:1170225" /note="vector for blue-light-induced gene expression" terminator complement(26..73) /note="T7 terminator" misc_feature complement(158..203) /note="multiple cloning site" promoter complement(339..387) /note="lambda pR promoter" CDS complement(561..1307) /note="lambda cI-LVA" /codon_start=1 /transl_table=11 /product="cI-LVA" /protein_id="AFH34992.1" /db_xref="GI:383460063" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE SCSVVGKVIASQWPEETFGAANDENYALVA" promoter complement(1332..1578) /note="FixK2 promoter" misc_feature 1951..3704 /note="YF1/FixJ bicistronic operon" CDS 1951..3093 /codon_start=1 /transl_table=11 /product="YF1" /protein_id="AFH34994.1" /db_xref="GI:383460065" /translation="MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQ GFVQMTGYETEEILGKNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMF WNELNIDPMEIEDKTYFVGIQNDITEHQQTQARLQELQSELVHVSRLSAMGEMASALA HELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVA RGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNL FRNALEAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTG MGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAADEMIGGLA" CDS 3090..3707 /codon_start=1 /transl_table=11 /product="FixJ" /protein_id="AFH34995.1" /db_xref="GI:383460066" /translation="MTTKGHIYVIDDDAAMRDSLNFLLDSAGFGVTLFDDAQAFLDAL PGLSFGCVVSDVRMPGLDGIELLKRMKAQQSPFPILIMTGHGDVPLAVEAMKLGAVDF LEKPFEDDRLTAMIESAIRQAEPAAKSEAVAQDIAARVASLSPRERQVMEGLIAGLSN KLIAREYDISPRTIEVYRANVMTKMQANSLSELVRLAMRAGMLND" rep_origin complement(5111..5793) /note="ColE1 origin" CDS 5839..6654 /note="KanR resistance marker" /codon_start=1 /transl_table=11 /product="KanR" /protein_id="AFH34993.1" /db_xref="GI:383460064" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin complement(6747..7202) /note="M13 origin" rep_origin complement(6879..7185) /note="F1 origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt 301 atatctcctt cttaaagtta aacaaaatta tttctagagc aaccattatc accgccagag 361 gtaaaatagt caacacgcac ggtgttactc tagtatataa acgcagaaag gcccacccga 421 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa 481 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag tagcgatcta 541 cactagcact atcagcgtta ttaagctact aaagcgtagt tttcgtcgtt tgcagcgcca 601 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg 661 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg 721 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc 781 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct cggcttggag 841 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct 901 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt 961 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac 1021 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat 1081 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca 1141 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca 1201 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt 1261 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatcta gtatttctcc 1321 tcttttctag actccgttgt gatgacgcat tggtacgcgg tatcgggagg ttcgaaaatt 1381 tcgagcgata tcttaagggg ggtgccttac gtagaacccc gtaggtcatg cccgaggccg 1441 gtcctggatg gcgcggcgga tacgcttgag caggttttcg tcgagaagcg gcttcaaaac 1501 cacgtctttt acgccggcct cggcggcccg ggtcgagatg ttttcgtccg gatagccggt 1561 gatcaggatc acgggcgtag atctcgatcc tctacgccgg acgcatcgtg gccggcatca 1621 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 1681 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 1741 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 1801 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 1861 gtcgagatcc cggacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc 1921 ccggaagaga gtcaattgag ggtggtgaat gtggctagtt ttcaatcatt tgggatacca 1981 ggacagctgg aagtcatcaa aaaagcactt gatcacgtgc gagtcggtgt ggtaattaca 2041 gatcccgcac ttgaagataa tcctattgtc tacgtaaatc aaggctttgt tcaaatgacc 2101 ggctacgaga ccgaggaaat tttaggaaag aactgtcgct tcttacaggg gaaacacaca 2161 gatcctgcag aagtggacaa catcagaacc gctttacaaa ataaagaacc ggtcaccgtt 2221 cagatccaaa actacaaaaa agacggaacg atgttctgga atgaattaaa tattgatcca 2281 atggaaatag aggataaaac gtattttgtc ggtattcaga atgatatcac cgagcaccag 2341 cagacccagg cgcgcctcca ggaactgcaa tccgagctcg tccacgtctc caggctgagc 2401 gccatgggcg aaatggcgtc cgcgctcgcg cacgagctca accagccgct ggcggcgatc 2461 agcaactaca tgaagggctc gcggcggctg cttgccggca gcagtgatcc gaacacaccg 2521 aaggtcgaaa gcgccctgga ccgcgccgcc gagcaggcgc tgcgcgccgg ccagatcatc 2581 cggcgcctgc gcgacttcgt tgcccgcggc gaatcggaga agcgggtcga gagtctctcc 2641 aagctgatcg aggaggccgg cgcgctcggg cttgccggcg cgcgcgagca gaacgtgcag 2701 ctccgcttca gtctcgatcc gggcgccgat ctcgttctcg ccgaccgggt gcagatccag 2761 caggtcctgg tcaacctgtt ccgcaacgcg ctggaagcga tggctcagtc gcagcgacgc 2821 gagctcgtcg tcaccaacac ccccgccgcc gacgacatga tcgaggtcga agtgtccgac 2881 accggcagcg gtttccagga cgacgtcatt ccgaacctgt ttcagacttt cttcaccacc 2941 aaggacaccg gcatgggcgt gggactgtcc atcagccgct cgatcatcga agctcacggc 3001 gggcgcatgt gggccgagag caacgcatcg ggcggggcga ccttccgctt caccctcccg 3061 gcagccgacg agatgatagg aggtctagca tgacgaccaa gggacatatc tacgtcatcg 3121 acgacgacgc ggcgatgcgg gattcgctga atttcctgct ggattctgcc ggcttcggcg 3181 tcacgctgtt tgacgacgcg caagcctttc tcgacgccct gccgggtctc tccttcggct 3241 gcgtcgtctc cgacgtgcgc atgccgggcc ttgacggcat cgagctgttg aagcggatga 3301 aggcgcagca aagccccttt ccgatcctca tcatgaccgg tcacggcgac gtgccgctcg 3361 cggtcgaggc gatgaagtta ggggcggtgg actttctgga aaagcctttc gaggacgacc 3421 gcctcaccgc catgatcgaa tcggcgatcc gccaggccga gccggccgcc aagagcgagg 3481 ccgtcgcgca ggatatcgcc gcccgcgtcg cctcgttgag ccccagggag cgccaggtca 3541 tggaagggct gatcgccggc ctttccaaca agctgatcgc ccgcgagtac gacatcagcc 3601 cgcgcaccat cgaggtgtat cgggccaacg tcatgaccaa gatgcaggcc aacagccttt 3661 cggagctggt tcgcctcgcg atgcgcgccg gcatgctcaa cgattgacaa ttgatgtaag 3721 ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt 3781 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 3841 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 3901 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 3961 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 4021 tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg 4081 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac 4141 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg 4201 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg 4261 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa 4321 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg 4381 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag 4441 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga 4501 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca 4561 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg 4621 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga 4681 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 4741 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 4801 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 4861 tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg 4921 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 4981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 5041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 5101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 5161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 5221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 5281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 5341 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 5401 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 5461 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 5521 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 5581 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 5641 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5761 acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac 5821 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga 5881 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 5941 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 6001 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 6061 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 6121 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct 6181 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 6241 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 6301 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 6361 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 6421 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 6481 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 6541 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 6601 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta 6661 attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6721 cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc 6781 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 6841 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 6901 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 6961 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 7021 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 7081 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 7141 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 7201 gtcccattcg cca //