LOCUS       pEMS1307               16482 bp    DNA     circular     16-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|546089574|
COMMENT     VNTDBDATE|546089574|
COMMENT     LSOWNER|
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COMMENT                             6.27457 6.27257 1 0.0214037) 0.0380608) 
COMMENT                      #138=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #98# 8 100) 
COMMENT                            (LOGPEN 0 0 153) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pEMS1307" "@N" 1 0 0.871165 0 
COMMENT                            -10 6.2441 1.47361 Nil) 
COMMENT                      #139=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #97# 21915980 100) 
COMMENT                            (LOGPEN 0 0 153) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "16482 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 3.19698 0.949103 Nil)) (CObjectList)) 
COMMENT               #140=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -182 100) 
COMMENT                     (CObjectList 
COMMENT                      #141=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -461 100) 
COMMENT                            (CObjectList 
COMMENT                             #142=(CScratch 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #104# 824 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.22713 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #143=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 21775284 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "MCS" "@N" 1 
COMMENT                                   -4.59516 14.1574 0 -10 2.2229 1.17389 #142#) 
COMMENT                             #144=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #104# 4063688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 6.19706 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #145=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 1550738791 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 5.8261 15.9178 0 -10 
COMMENT                                   7.99245 1.17389 #144#) 
COMMENT                             #146=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.92036 
COMMENT                                    6.1944 1 0.082322) 0.8 1.8 0) 
COMMENT                             #147=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 35954144 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   4.83712 13.6759 0 -10 2.67247 1.17389 #146#) 
COMMENT                             #148=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #104# 897 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.92036 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #149=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 22130508 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 12.6777 
COMMENT                                   12.8827 0 -10 10.0405 1.17389 #148#) 
COMMENT                             #150=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.90666 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #151=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 4063864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   9.43243 11.2902 0 -10 1.99811 1.17389 #150#) 
COMMENT                             #152=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.80047 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #153=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 1869442930 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 15.758 
COMMENT                                   5.83244 0 -10 11.0645 1.17389 #152#) 
COMMENT                             #154=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.21533 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #155=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 39423 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "FFRT (F5 mutant)" "@N" 1 -4.66535 15.8618 0 
COMMENT                                   -10 7.99245 1.17389 #154#) 
COMMENT                             #156=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #104# 828 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.87697 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #157=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FFRT" "@N" 1 
COMMENT                                   12.1068 8.74866 0 -10 2.52262 1.17389 #156#) 
COMMENT                             #158=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #104# 4063688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 2.34341 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #159=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 22048464 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce-I" "@N" 1 
COMMENT                                   -10.4191 -8.71165 0 -10 3.02214 1.17389 #158#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #160=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -251 100) 
COMMENT                            (CObjectList 
COMMENT                             #161=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #112# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    0.00228365 0.467387 1 0.082322) 0.8 1.8 1) 
COMMENT                             #162=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -7.79019 11.9216 0 -10 2.87229 1.17389 #161#) 
COMMENT                             #163=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #112# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.34569 2.88844 1 0.082322) 0.8 1.8 0) 
COMMENT                             #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -7.9442 -10.454 0 -10 2.87229 1.17389 #163#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #165=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -321 100) 
COMMENT                            (CObjectList 
COMMENT                             #166=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 0.885295 
COMMENT                                    2.33617 1 0.082322) 0.8 1.8 0) 
COMMENT                             #167=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 22126292 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.924 1.97314 
COMMENT                                   0 -10 10.4901 1.17389 #166#) 
COMMENT                             #168=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.88653 4.27423 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 21892788 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 11.0558 -10.454 
COMMENT                                   0 -10 10.4901 1.17389 #168#) 
COMMENT                             #170=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #100# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 4.27423 
COMMENT                                    5.77916 1 0.082322) 0.8 1.8 0) 
COMMENT                             #171=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #96# 37772696 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.381 
COMMENT                                   1.74233 0 -10 14.9609 1.17389 #170#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #172=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1023 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #173=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1371 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #174=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -953 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 4064080 14 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     10345..14156
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(15255..16476)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     8894..10319
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     5253..8898
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     1299..5252
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    122..152
                     /vntifkey="21"
                     /label=MCS
     misc_feature    201..211
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    208..927
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    928..963
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    964..1036
                     /vntifkey="21"
                     /label=NLS
     misc_feature    1243..1293
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    153..200
                     /vntifkey="21"
                     /label=FFRT\(F5\mutant)
     misc_feature    1042..1089
                     /vntifkey="21"
                     /label=FFRT
     misc_feature    10326..10343
                     /vntifkey="21"
                     /label=I-Sce-I
BASE COUNT     4423 a      3688 c      3675 g      4696 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccac gcgtggcgcg ccgaagttcc tattccgaag ttcctattct 
      181 tcaaaaggta taggaacttc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 
      241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 
      301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 
      361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 
      421 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 
      481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 
      541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 
      601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 
      661 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 
      721 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 
      781 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 
      841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 
      901 ctcggcatgg acgagctgta caagttattt ctgaactgct gcccgggctg ctgcatggaa 
      961 ccggatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa 
     1021 aagaagagaa aggtaatata agaagttcct attccgaagt tcctattctc tagaaagtat 
     1081 aggaacttca gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 
     1141 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 
     1201 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1261 caaatttcac aaataaagca tttttttcac tgcgaattct cgtaaaactc ttcatggcag 
     1321 tagttattat tctctctctc tctctttttc ttttttcttg agacaggata tttctctgtt 
     1381 gcccaggctg gagtgcagtg gcacagtctt ggctcactgc agcctggacc tcctgggctc 
     1441 aagccatcct cccacctcag cctcccaagt agctggggct acaggcacat ggccaccagg 
     1501 cccagataat ttttcatttt tgtagagact gagtctcacc atgttaccca ggtttattat 
     1561 tctcattttt tagatgaaga gactgaggtc cagagaagct caatgacttg cctagtttta 
     1621 caaatctcct gccatcacat acccctcagc gtccttaata agagggaggc caccaactat 
     1681 gtgctgggca ctgtggtgga tgctggagct atagggttga gtatataaga aatggtgttg 
     1741 ctggagcaac tgttgcttgc ttacctgacc tatctgagaa ttaattagca ggggaacata 
     1801 tttttgtttt cagattcaat ataagaactt gtgtgggcaa aaataaagat cagtagtaat 
     1861 aacagtagtt cccatttgct gactgtactg tcctaagtgc atatatatat acatacacac 
     1921 acgcatacct atactcctct aatactcaaa atgatcctgt ttatgtattg ttaatatgct 
     1981 cattttattt ttaaattttt atttattttt atttttattt atttttgaga cggagtctca 
     2041 ttctgtcgcg gaggctgaag tgcagtggtg cgatctcagc tcagtgcgac ctccgcctcc 
     2101 cgggttcaag tgattctcct gcctcagcct ccggactagc tgggattaca ggcgcccgcc 
     2161 tccacgccca gctaattttt gtatttttag tagagatggg gtttcgccat gttggccagg 
     2221 ctggtctcgt actcctgacc ttgagtgatc cacctgcctc ggcctcccaa agtgctggga 
     2281 ttacaggcat gagccaccgc gccgggctaa tatgctcatt ttagtgaggc aaaaatagag 
     2341 gctcagagtc tgatttgtac aaaactacag agcagttaag tgtcctctca gatgtgtacc 
     2401 ctgatctggg tgactctagg actctaggtc tcaactgtta caaccagtta agggtttggg 
     2461 gaagcactgg gccaagagtc aggaaaatgg aagccacagg tagtgcaagg tcttgggaat 
     2521 gggacgtctg gtccaaggat tcacgcgatg actggaaccc gaagagccgg ggcccggttt 
     2581 acggccgcca tgaagcaacg cgcgccggta ggtttgggaa tcagggagcc ctctgaatag 
     2641 gagactgagt tgggagggaa aggggcttcg ctgggggagc ctcggcttct tctgggagaa 
     2701 aattcccacg gctacctagt gagcctgcaa actggtaggc gccggcgtag gcgcgcgggc 
     2761 ggggccgggg gcggggcctg cggggcgtgg cggggcgggc agagggcggg gcctgcttct 
     2821 cctcagcttc aggcggctgc gacgagccct caggcgaacc tctcggcttt cccgcgcggc 
     2881 gccgcctctt gctgcgcctc cgcctcctcc tctgctccgc caccggcttc ctcctcctga 
     2941 gcagtcagcc cgcgcgccgg ccggctccgt tatggcgacc cgcagccctg gcgtcgtggt 
     3001 gagcagctcg gcctgccggc cctggccggt tcaggcccac gcggcaggtg gcggccgggc 
     3061 cctgaggcgc gggatcgatc catgacaccc ccccgccccc catatctcac tataattcac 
     3121 tgtcaaatgg cttttatgat ttggagtttg gggatacttt taaagacagg gtcttggtag 
     3181 ctcctagatg gcttgggatt cacttagtaa cccttgctgg cctaaaattc aggaacttct 
     3241 acctcatctt cccaagtact gggttagtag ggatgtccag catatttagc ttgctttaat 
     3301 gattttgctg tacttttttc tgtgtgtaca ggtgcatgta tatttataaa actaacataa 
     3361 tatattttac ccccaaagta ccataaccct aattaatgct attaaatgtt ttgtatgctg 
     3421 ggctctgggc atactaatat aaccaatatt atacaatgca aaacaatttt ggaatatctt 
     3481 ctgtaaaatt atcttattag taagttgtaa ccatctcaga cttctaagtt tgagtttgta 
     3541 aaggatcagt tttttttggt gacctacaca cacacacaca cacacacaca cacacacaca 
     3601 cacagggggg tggattctga gaatttagag aatttagatg gttaatacca gtttttattt 
     3661 ttattctgtg tgatatttat ttattaagat ttatttattt attacatgta ggtacactgt 
     3721 atctgtcttc agacacacca gaagagggca tcagatctcg ttacagatgg ttgtgagtca 
     3781 ccatgtggtt gctgggattt gaactcagga cctctggaag aacagtcagt gctcttaacc 
     3841 gttgagccat ctcaccagcc ccgtgatatt ttatttaact tactagaata tactgtgtta 
     3901 aaacatattt taggggattg gggagatggc ccattctgta aagtgtctgc catgtcatct 
     3961 tcaggacctg cgtttgtaac cccagcatgg agaagacaga gacagggaga cccctgggct 
     4021 tcctggcaag ctagactagc aaatttggtt aactaggttc aatgagagac tcagtctcaa 
     4081 aacataagaa gtgaattttt tagacccaat ttcaatctct gaactccata cgtatgcaca 
     4141 tgtacctgca tataaatata tatacacacc cataaacaca tgcacacaca aaaaatggtt 
     4201 caaaagttct taaaatgtat aagagtagaa agtgtagagc cgggagtggt ggcgcacgcc 
     4261 tttaatccca gcacttggga gcagaggcag gtggatttct gagttcgagg ccagcctggt 
     4321 ctacagagtg agttccagga cagccagagc tatacagaga aatcctgcct caaaaaacca 
     4381 aaaataaagt gtatatacta atctttcatg cctaaaatat ttatttttgc cacatttttc 
     4441 atacctcttt aatatttttc tttttctctt tgtgcttcaa aggaaatttt gtgtcatgtt 
     4501 ttacttttca ttcttgactc tattcagcag taagacgcag catggctttc ttagagaagt 
     4561 ataatctatt acaaaacggt aacatttaac tgctctacat accctcttgt agctaaattc 
     4621 ctctgttaaa ctaagactta ttatctaaac gtaattaaat gttgatatct tataatgctc 
     4681 cactttgaaa cagctgtctt tcttttgcag attagcgatg atgaaccagg ttatgaccta 
     4741 gatttgtttt gtatacctaa tcattatgcc gaggatttgg aaaaagtgtt tattcctcat 
     4801 ggactgatta tggacaggtt agtaagacct cgattgaagt tatttttaga gggtgtgtgt 
     4861 gtggtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaagagag agaggagagg 
     4921 catgtgtgat atatatgtga agtgtattgc atgtacatgg aatccagaga aggacattag 
     4981 gtgtcctact ctgttgctct cttattccct tgaatcagtg tctcttgctg aacctggggc 
     5041 atacatactg atttggctag gctgcctggc tagcaagacc cagggatctg cttgtctctg 
     5101 ccccccagtg ctgacgttac aagcaggtgc aaccacatct ggtttttata tgggtactgg 
     5161 gggatctgaa cttagggcct gatgcgtgcc caagtgctct cacccgccaa gccacctctc 
     5221 cagccaaagg gttttttttc ttgtattaat tctaacttag caactgttga gtagttgatg 
     5281 tttgaatata agctaagcca acttattaga ataaatgttt tctatttttt acttgtatgt 
     5341 gtgagtgcct gcatgtatgt atgtgaagca tatgtattcc aggatgtcag aattgtgatg 
     5401 gattccctgg aactggagtt acagaccatt gtgaaccact gtgtatgggt gctgggaact 
     5461 gaactttagg tcctctgcaa aaacagtaag gatcttaact gataaactat ctttctagcc 
     5521 ctaaggcttt ctacttttaa agttcgtttc tctttctctc tctctctctc tctctctctc 
     5581 tctctctccc tctccctccc tcctcctcct cctcctcttt cccccctctc gcctcctctt 
     5641 tctctctccc ctccctctct ctctctcaca cacacacaca gacagagaga ggagagagaa 
     5701 aggaagcctt actaattaat tacctagttt tagacataac tagtgggaat ccatagtaag 
     5761 taaatcaaca ttcttgaaga gaggttagaa gaattggctc tcgttgtcca tcgaggctgt 
     5821 ataagattat tcttctgttt tctcagctta ccatacatat tttctacgta gacaatacca 
     5881 attcaaatac actgtacgta aagtatatgc gcacacacac acacacacac acacacacac 
     5941 acacacaagt tacatattgt catatagtac taaaatttat ggactttttc atattaatac 
     6001 atctagagct atcatgtttt ttatctttta aaaattactt tacggggcta cagagatgtc 
     6061 tcagcagtta ggagcactga ctgctcttcc agaggtcctg agttcaattc ccagcaacca 
     6121 catggtggct cacaaccatc tgtaatggga tctgatccac tcttatggtg tgtctgaaga 
     6181 cagtgacagt gtactcatat acatgaaata aatctttaaa aaattacttt accatcaggg 
     6241 tatatatata tatatatatg tgtgtgtgtg tgtgtgtgtg tatgaagtat ttgctatttg 
     6301 agagggtctc actgtttagc tcagtctggc ttggaacgtt ctgtatagcc cagactggcc 
     6361 tcaactcagc agtccccctg aaaggctctc atatgtggag attacaggta tgtgctactg 
     6421 tgcccagccc tacatgttac tttgtttttg aaatagggta tcaaatagct tagactggct 
     6481 tctaagtcag tgtgtagcta aggatggtct gagcttctga acctgctact tcaactcctg 
     6541 gtgtgccatc atggccagtt tgcacagttg tgagagtaga actcaggacc tcctatgtgt 
     6601 tactagacat gttctctacc agctgagcta catcccaatt cgcctacatt tacttttatt 
     6661 ttttaaaaga tttatttatg tatacagcac tctgcctgca tgtacatctg catgccagaa 
     6721 gagggcacca gatctcatca tacatagttg tgagccacca tgtggttgct ggggattgaa 
     6781 ctcagggcct ctggaagagc agtcagtgct cttaacctct gagccatctc tgtagccccc 
     6841 tacatttatt ttaaagatta taggctattc tattcataga tgtgccataa ctgatttaac 
     6901 cagatcttca ttattttgta cttagtagag tatattagag ggaatgaatc ttaaatacac 
     6961 attttgacac ttgctcagct ttagacaagt aaaaatcact ggtcaaggaa atggtgctta 
     7021 gtggatgctt ctgaactgtc aaattgtcct ccaacaagtt acatcatttt atacttccaa 
     7081 cgttatgtga gagttcattt ttctttaggc tcttctagta ttttatattt gagaaataaa 
     7141 atttaaccaa aatttgccaa tctagtaagc ttgtttttat ttgtatcttt aaattttatg 
     7201 atagatgtta tagtgtactc tcctctcccc tcccctcccc tctcctcctc agcaggatct 
     7261 tgttatgtag ccctaaggga tctaatactt actctgtagt ccaggctgtc cttcagctca 
     7321 tgagacacac catcatgctt ggcatgtttc ttgttctgaa aggtatctgg ttttaatttt 
     7381 ggttgagctg gtgtgttggt gtatatctgt aatctcagca caggcaagta gagacaagga 
     7441 ttatgattac agtccagcct gggctattta gagggattct ctttaatctt tcctcatgcc 
     7501 ccaaaatctt acctttggta tatgaaaaat agtctccact tctgcaaaat attgctttat 
     7561 gaagtaagaa ttaattccct tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg 
     7621 taagaagtat ttgttataaa agataaatat tcagaatctt ctttttaatt cctgatttta 
     7681 tttctatagg actgaaagac ttgctcgaga tgtcatgaag gagatgggag gccatcacat 
     7741 tgtggccctc tgtgtgctca aggggggcta taagttcttt gctgacctgc tggattacat 
     7801 taaagcactg aatagaaata gtgatagatc cattcctatg actgtagatt ttatcagact 
     7861 gaagagctac tgtgtaagta taattaactt ataattaaaa aaatagggcc attctagttt 
     7921 tatctatatt ttttttaaac ttgtgcaaac tatgctacat aatcaactta cttgattatg 
     7981 taatatatat agtaatagtt ggaattacta ttattacata tttatttttc tgtgctgggg 
     8041 tagaacccag ggaactaagt actgtaataa ccgagcttca ttcatcctag tcatttttaa 
     8101 tttttttttt tttttttgtt ttttcgagac aaggtttctc tgtgtagccc tggctgtcct 
     8161 ggaactcact ctgtagacca ggctggcctc gaactcagaa atctgcctgc ctctgcctcc 
     8221 cagtgctgag attaatgacg tgcaccacca ctgcctggca tttttttaaa gttttaattt 
     8281 gagacccttt ctcactaact tgctcacact atcctcgaac tcattacgtg ggctggtcta 
     8341 gaaatttcag tcaacttgcc tcagcttctc aagtagctgg gattacaggc aggtgctacc 
     8401 acttctggcc atgatcattg ttgttgttgc tattattatt attattattt cactgatact 
     8461 tcatatcaca acacagaata gtaactgtag ttcatgacca acaaaaaagt tgtacagttt 
     8521 ctctgttgtc ttattttgat ctggattttg atttgtttca tggcactggg caggctaatc 
     8581 tcaaactgca tggctcaaat agtccttctg tcttaatctc ccatgttctg gaagtatagg 
     8641 tatcactaca cctagctgta ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga 
     8701 atgaggcgga gagatggctc agcagtcaca agtacttgta gaggactggg gtttggttac 
     8761 tagcacctca aagatggctt acaattatct gtagctccaa ttccagagga tctgtgtggt 
     8821 aaagctgcat gtatatgggc atgcaggtaa aactgctaaa catataaaaa tagctcagtc 
     8881 ttaagagaat tggtcgactt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     8941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     9001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     9061 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 
     9121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     9181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     9241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     9301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     9361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     9421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     9481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     9541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     9601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     9661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     9721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     9781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     9841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     9901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     9961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
    10021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
    10081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
    10141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
    10201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
    10261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagg 
    10321 tcgagtaggg ataacagggt aatggatcct cgttgggcac ctgcactcat gtacgcaggc 
    10381 ccacatatag atgtacacac gcatatcatt caaaatagaa attatttata caagtagtgc 
    10441 gttggagagc tggctcagga gttgttaatg cttgctaccc ttgcaaagga cccaagttca 
    10501 atttccagca cctgtgttgg gcatctcaca accacatgta aatctagttt caggggattt 
    10561 cacccctctt ctgaactctt caggcactta tacccacaaa tggacatctc cacatagaga 
    10621 cacatacttt taaaaaagta atctagggtc tagagaaatg gttatagacc acggactggg 
    10681 atcagttccc agaagccaca tcctgtggct cacaattgcc tgtgaatcaa gttctagatc 
    10741 tggtggcctc gtctagcctg cagtcatcaa caaacacatg cacacatcca tacacacata 
    10801 atttaaaaga aagcgaaagt aacctataat gctaaacaaa gcacattctc agtgtagact 
    10861 gcagtggtga tgaaccttga aaatatatta atagaagcca gacacgtaag tcacaaatgg 
    10921 tataactcaa tttatatgaa attcccagag agtccaaagt taaaaaaaac aaaaaggcag 
    10981 attattggtt gtcagtagct acagagatgg gacattgaag atattaatag gtatagggtt 
    11041 gaggattcat ggcctttccc tcttccatgt tagcatgtct attgttgtta accttattca 
    11101 actcatgtta gggcaatcat attgatgaga cttaatgagt atagcttgac attcctagaa 
    11161 gacagagtct cacatcagac tctctgatcc tctgactctt agaatctttc aacaccctct 
    11221 tctgaaatga tctctgagcc tttggttgtg actgaattat gagaaagaat tttatgttga 
    11281 atgaatttta cttcagcatt tttacaaaac tgaagctggg gatggcgttt catgcgtgca 
    11341 gtccctacca tttgcctata agcctggagg aggcagaagt agaattaggc ttttcaggga 
    11401 catcttcagc tgcatcatga gcttgaggac aacttgagct acataagatt cccatcccca 
    11461 cccccacaca cacacacaca tctagtatgt acctagtatg cagtcttttc atatgtttaa 
    11521 aatgtagaaa ggtatatgtg aagtctccct tttccccaca acttagtccc tccaaagtat 
    11581 tgcatcagtt tccttgtaca tgattttatg catatataag atacactatt tctctctctc 
    11641 tctctctcct tccctccttc cctccaacct ctgggtatga gttgagggtt atgtatgttt 
    11701 caagttgtaa taagaattgc cactcaaaca tttcatccca ttttaagtta agtttgtctg 
    11761 attttccact ttagaagggc agccatgatg gctcatgcct ataatcccat cctagcactt 
    11821 gtaagcctaa tacataagac tggaggattg tcatcagctt gaggctagcc tggtctacct 
    11881 aatgagttcc agccagccaa gagtagaata taagaccctg tctccaaaaa atgtaccaaa 
    11941 ggggctggag gggtaactca gtggttaaga acagtagctg ctcttccaga ggacctgggt 
    12001 tcaattccca gcacccacat ggcagcttgc aactatctgt aactgtccag ctttgggata 
    12061 tccgacacca ttataccaat tacataaaat gaaaaaattg taaaatgtgc taaaatgagt 
    12121 ggggaggggg agcatggtgt ggttctgtgg gcttgggatt ttgccatgaa tccatgacta 
    12181 tatctcagca atatgtggat tatttctgga aacctgtggg gcatgggtgt atgggaagag 
    12241 gggagagaga gagagcccgc atccagccag agttcctgtg ctctgggcag gcggacgtgg 
    12301 gaggactgct ggacgatttc cacttggccc tgggtgggca tctggctgtg tgaagccact 
    12361 gaccccactc agcggggggt agataagggg catttcccat accaggttct cagactccag 
    12421 cattccacgc tggatacagc agaagagctg agaacagaat aggggcccca gggagacact 
    12481 ctcgtcccag agataaagaa ggcagaggga gagagattcc tacgcaggcg agaggcctta 
    12541 gtctgggcct tgatgaacag agacggtcta tggttttaga gctttattgt agaaaggcag 
    12601 ggggaaagag agaaggtaga aagagagaga ccagccatgg ccaagaggag agaagggggg 
    12661 aaaggagaga gagaagaaag gctagagagt aagaaaggtt aaagagacag aagtaaaagc 
    12721 ttagagtaag agagagagag agagcaagag ggagagtaag aaaagtaaaa gcttaagaga 
    12781 gcgaggaggg gccagcccct ctaatagtgg gcttgttatc ttgttgttgc taggtaactg 
    12841 tgaagcgtct agcctgaagg ccagaagctt gggacattgt ctatgtgact gaaagccatg 
    12901 agcctctact gtgggggctg tggaggtggt aacttggaca ggagccaggg gttccaggag 
    12961 acatgaggga atgccttctg tcccatgtag gtaggaatta ccaccaccgg ctttcaccgt 
    13021 agttcactgg agactgtttg tacatagccc attgccccac agaaaccagg cactcactgt 
    13081 ttcatttgca taagaattag aaggctttat tttggctggg attggtgact tgccccagaa 
    13141 attccagctt ttgggaagcc gaatcctgga gaaccaggag ttcaaggtca tcctcaggta 
    13201 cctcatttgt tcaagaccag cctgtgctac catgagacct tgtccctact cccctcaacc 
    13261 ccaaaacacg ctgttttcat tttataggtt gtactaacca tggaggatgg tcatttgcct 
    13321 ttctcttgat ttcagaagcg tagcgcgggc tttgcaccat gatctagtgt ttccagatag 
    13381 gcttgcctct ccacagccag gcttatcctg cttcagtccc atgtttggca agggcaacat 
    13441 atgactgaag aacttgcata gccttatggt gtatcatgac tcttagcccc agtttataac 
    13501 tgtctaacag ctggtggctt ttgtgaaaaa ggaaagcatc atttgaactt ttaacttttc 
    13561 agctgtttgt tttgactttg cataaatact gagactagct aggaaatgag cttttgtttc 
    13621 atttgctaaa aatgattttt ttttcatgag gcaatttcaa gcaatttgaa tttagcaatc 
    13681 tctacttgtc tgaaaagaag ttattaatta aaaaaaaaca aacaaacagg aacctattat 
    13741 gctggctagt cacatgaaag aatccaaatg aacacagtca aggctgaatc aacacatgaa 
    13801 attattttct aattccccaa attacattta tgctaattag tatgtagtaa aacattttaa 
    13861 ttgtttccaa tgttaaacat tacagaaata attttgatgt tttcctaata acccagcctt 
    13921 tgatcatgtt tgcatttatt tgattgttgt cagtttatta aataattaag taagagcaac 
    13981 aagataaaat gacttcatga gagtcctttt ctgtaactta gaacgtcagt agtcatagga 
    14041 actgcggtcg ttcccttacc tttcagaagt aggctaaaga gttgaacgca aaggtggttt 
    14101 ttcgaatctt cctgatcctt aagcccccac taggtttata ttctatattt gatatcggat 
    14161 tcgaacccct cgagcttagg atccggaacc cttaatataa ctaagttatt aggtccctcg 
    14221 aagaggttca ctagtactgg ccattgcggc cgcttcgagg ctgcctcgcg cgtttcggtg 
    14281 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
    14341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
    14401 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 
    14461 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 
    14521 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
    14581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
    14641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
    14701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
    14761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
    14821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
    14881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
    14941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
    15001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
    15061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
    15121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
    15181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
    15241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
    15301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
    15361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
    15421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
    15481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
    15541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
    15601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
    15661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
    15721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
    15781 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
    15841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
    15901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
    15961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
    16021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
    16081 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 
    16141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
    16201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
    16261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
    16321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
    16381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
    16441 aggggttccg cgcacatttc cccgaaaagt gccacctgac gg 
//
