LOCUS       pRE118                  6561 bp    DNA     linear       14-FEB-2013
FEATURES             Location/Qualifiers
     misc_feature    complement(4885..6561)
                     /note="OriT"
     CDS             955..2376
                     /note="sacB1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
                     HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
                     IKGKKTSVVKDSILEQGQLTVNK"
     misc_feature    806..833
                     /note="sacB-F (Curtis   08)"
     misc_feature    1563..1586
                     /note="PrMK096"
     misc_feature    1602..2491
                     /note="Pr96 seq"
     misc_feature    complement(2350..2376)
                     /note="sacB-R (Curtiss 08)"
     misc_feature    2252..2275
                     /note="PrMK097"
     misc_feature    complement(2490..2499)
                     /note="PrMK066"
     misc_feature    2294..3142
                     /note="Pr97seq"
     misc_feature    1..942
                     /dnas_title="Pr71seq1"
                     /note="Pr71seq1"
     misc_feature    complement(2501..3142)
                     /note="Pr99seq"
     misc_feature    418..440
                     /note="PrMK072/081"
     misc_feature    485..490
                     /note="PrMK066"
     misc_feature    complement(490..490)
                     /dnas_title="Pr97seq1"
                     /note="Pr97seq1"
     misc_feature    455..1324
                     /note="Pr72seq"
     misc_feature    44..61
                     /note="PrMK071/080"
     misc_feature    complement(65..68)
                     /note="Pr98seq1"
     misc_feature    complement(1..68)
                     /note="OriT"
     misc_feature    complement(104..484)
                     /note="R6K-OriV"
     misc_feature    complement(2360..2377)
                     /note="PrMK111"
     CDS             complement(3458..4273)
                     /note="aph gene (Kanamycin-resistance)"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     source          1..6561
                     /dnas_title="pRE118 New"
ORIGIN      
        1 cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc
       61 ggctggatcg GCACGATGCG TCCGGCGTAG AGGATCTGAA GATcagcagt tcaacctgtt
      121 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
      181 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
      241 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
      301 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
      361 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
      421 agaagccctt agagcctctc aaagcaattt tcagtgacac aggaacactt aacggctgac
      481 atggGAATTC tGATCCTTTT TAACCCATCA CATATACCTG CCGTTCACTA TTATTTAGTG
      541 AAATGAGATA TTATGATATT TTCTGAATTG TGATTAAAAA GGCAACTTTA TGCCCATGCA
      601 ACAGAAACTA TAAAAAATAC AGAGAATGAA AAGAAACAGA TAGATTTTTT AGTTCTTTAG
      661 GCCCGTAGTC TGCAAATCCT TTTATGATTT TCTATCAAAC AAAAGAGGAA AATAGACCAG
      721 TTGCAATCCA AACGAGAGTC TAATAGAATG AGGTCGAAAA GTAAATCGCG CGGGTTTGTT
      781 ACTGATAAAG CAGGCAAGAC CTAAAATGTG TAAAGGGCAA AGTGTATACT TTGGCGTCAC
      841 CCCTTACATA TTTTAGGTCT TTTTTTATTG TGCGTAACTA ACTTGCCATC TTCAAACAGG
      901 AGGGCTGGAA GAAGCAGACC GCTAACACAG TACATAAAAA AGGAGACATG AACGATGAAC
      961 ATCAAAAAGT TTGCAAAACA AGCAACAGTA TTAACCTTTA CTACCGCACT GCTGGCAGGA
     1021 GGCGCAACTC AAGCGTTTGC GAAAGAAACG AACCAAAAGC CATATAAGGA AACATACGGC
     1081 ATTTCCCATA TTACACGCCA TGATATGCTG CAAATCCCTG AACAGCAAAA AAATGAAAAA
     1141 TATCAAGTTC CTGAgTTCGA TTCGTCCACA ATTAAAAATA TCTCTTCTGC AAAAGGCCTG
     1201 GACGTTTGGG ACAGCTGGCC ATTACAAAAC GCTGACGGCA CTGTCGCAAA CTATCACGGC
     1261 TACCACATCG TCTTTGCATT AGCCGGAGAT CCTAAAAATG CGGATGACAC ATCGATTTAC
     1321 ATGTTCTATC AAAAAGTCGG CGAAACTTCT ATTGACAGCT GGAAAAACGC TGGCCGCGTC
     1381 TTTAAAGACA GCGACAAATT CGATGCAAAT GATTCTATCC TAAAAGACCA AACACAAGAA
     1441 TGGTCAGGTT CAGCCACATT TACATCTGAC GGAAAAATCC GTTTATTCTA CACTGATTTC
     1501 TCCGGTAAAC ATTACGGCAA ACAAACACTG ACAACTGCAC AAGTTAACGT ATCAGCATCA
     1561 GACAGCTCTT TGAACATCAA CGGTGTAGAG GATTATAAAT CAATCTTTGA CGGTGACGGA
     1621 AAAACGTATC AAAATGTACA GCAGTTCATC GATGAAGGCA ACTACAGCTC AGGCGACAAC
     1681 CATACGCTGA GAGATCCTCA CTACGTAGAA GATAAAGGCC ACAAATACTT AGTATTTGAA
     1741 GCAAACACTG GAACTGAAGA TGGCTACCAA GGCGAAGAAT CTTTATTTAA CAAAGCATAC
     1801 TATGGCAAAA GCACATCATT CTTCCGTCAA GAAAGTCAAA AACTTCTGCA AAGCGATAAA
     1861 AAACGCACGG CTGAGTTAGC AAACGGCGCT CTCGGTATGA TTGAGCTAAA CGATGATTAC
     1921 ACACTGAAAA AAGTGATGAA ACCGCTGATT GCATCTAACA CAGTAACAGA TGAAATTGAA
     1981 CGCGCGAACG TCTTTAAAAT GAACGGCAAA TGGTAtCTGT TCACTGACTC CCGCGGATCA
     2041 AAAATGACGA TTGACGGCAT TACGTCTAAC GATATTTACA TGCTTGGTTA TGTTTCTAAT
     2101 TCTTTAACTG GCCCATACAA GCCGCTGAAC AAAACTGGCC TTGTGTTAAA AATGGATCTT
     2161 GATCCTAACG ATGTAACCTT TACTTACTCA CACTTCGCTG TACCTCAAGC GAAAGGAAAC
     2221 AATGTCGTGA TTACAAGCTA TATGACAAAC AGAGGATTCT ACGCAGACAA ACAATCAACG
     2281 TTTGCGCCcA GCTTCCTGCT GAACATCAAA GGCAAGAAAA CATCTGTTGT CAAAGACAGC
     2341 ATCCTTGAAC AAGGACAATT AACAGTTAAC AAATAAAAAC GCAAAAGAAA ATGCCGATAT
     2401 CCTATTGGCA TTTTCTTTTA TTTCTTATCA ACATAAAGGT GAATCCCATA TGAACTATAT
     2461 AAAAGCAGGC AAATGGCTAA CCGTATTCCT AACCTTTTGg TAATGACTCC AACTTATTGA
     2521 TAGTGTTTTA TGTTCAGATA ATGCCCGATG ACTTTGTCAT GCAGCTCCAC CGATTTTGAG
     2581 AACGACAGCG ACTTCCGTCC CAGCCGTGCC AGGTGCTGCC TCAGATTCAG GTTATGCCGC
     2641 TCAATTCGCT GCGTATATCG CTTGCTGATT ACGTGCAGCT TTCCCTTCAg GCGGGATTCA
     2701 TACAGcGGCC AGcCATCCGT CATCCATATC ACCACGTCAa AGGGTGACAG CAgGCTCATA
     2761 AgACGCCCCA GCGTCGCCAT AGTGCGTTCA CCGAATACGT GCGCAACAAC CGTCTTCCGG
     2821 AGAcTGTCAT ACGCGTAAAA cAGCCAGCGC TGGCGCGATT TAGCCCCGAC ATAGCCCCAC
     2881 TGTTCGTCCA TTTCCGCGCA GACGATGACG TCACTGCCCG GCTGTATGCG CGAGGTTACC
     2941 GACTGCGGCC TGAGTTTTTT AAGTGACGTA AAATCGTGTT GAGGCCAACG CCCATAATGC
     3001 GGGCTGTTGC CCGGCATCCA ACGCCATTCA TGGCCATATC AATGATTTTC TGGTGCGTAC
     3061 CGGGTTGAGA AGCGGTGTAA GTGAACTGCA TGaattcccg ggagagctcg atatcgcatg
     3121 cggtacctct agaagaagct tgGGATCCGT CGACCTGCAG GGGGGGGGGG GCGCTGAGGT
     3181 CTGCCTCGTG AAGAAGGTGT TGCTGACTCA TACCAGGCCT GAATCGCCCC ATCATCCAGC
     3241 CAGAAAGTGA GGGAGCCACG GTTGATGAGA GCTTTGTTGT AGGTGGACCA GTTGGTGATT
     3301 TTGAACTTTT GCTTTGCCAC GGAACGGTCT GCGTTGTCGG GAAGATGCGT GATCTGATCC
     3361 TTCAACTCAG CAAAAGTTCG ATTTATTCAA CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA
     3421 TGCTCTGCCA GTGTTACAAC CAATTAACCA ATTCTGATTA GAAAAACTCA TCGAGCATCA
     3481 AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT
     3541 TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC
     3601 GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA
     3661 TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA
     3721 GCTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT
     3781 CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA CGAAATACGC
     3841 GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG
     3901 CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG
     3961 TTTTCCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT
     4021 TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA
     4081 CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC
     4141 CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC
     4201 CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT
     4261 GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT TTTATTGTTC
     4321 ATGATGATAT ATTTTTATCT TGTGCAATGT AACATCAGAG ATTTTGAGAC ACAACGTGGC
     4381 TTTCCCCCCC CCCCCTGCAG GTCGACGGAT CCctgtcaga ccaagtttac tcatatatac
     4441 tttagattga tttaaaactt catttttaat tttggtgcac tctcagtaca atctgctctg
     4501 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4561 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4621 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4681 atcaccgaaa cgcgcgaggc agcaaggaga tggcgcccaa cagtcccccg gccacggggc
     4741 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     4801 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     4861 cggccacgat gcgtccggcg tagaGGATCc tttttgtccg gtgttgggtt gaaggtgaag
     4921 ccggtcgggg ccgcagcggg ggccggcttt tcagccttgc ccccctgctt cggccgccgt
     4981 ggctccggcg tcttgggtgc cggcgcgggt tccgcagcct tggcctgcgg tgcgggcaca
     5041 tcggcgggct tggccttgat gtgccgcctg gcgtgcgagc ggaacgtctc gtaggagaac
     5101 ttgaccttcc ccgtttcccg catgtgctcc caaatggtga cgagcgcata gccggacgct
     5161 aacgccgcct cgacatccgc cctcaccgcc aggaacgcaa ccgcagcctc atcacgccgg
     5221 cgcttcttgg ccgcgcggga ttcaacccac tcggccagct cgtcggtgta gctctttggc
     5281 atcgtctctc gcctgtcccc tcagttcagt aatttcctgc atttgcctgt ttccagtcgg
     5341 tagatattcc acaaaacagc agggaagcag cgcttttccg ctgcataacc ctgcttcggg
     5401 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     5461 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     5521 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     5581 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag
     5641 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg
     5701 tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc
     5761 ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat
     5821 gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg
     5881 aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc
     5941 ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc
     6001 cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat
     6061 tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtgaagta
     6121 catcaccgac gagcaaggca agaccgagcg cctgggtcac gtgcgcgtca cgaactgcga
     6181 ggcaaacacc ctgcccgctg tcatggccga ggtgatggcg acccagcacg gcaacacccg
     6241 ttccgaggcc gacaagacct atcacctgct ggttagcttc cgcgcgggag agaagcccga
     6301 cgcggagacg ttgcgcgcga ttgaggaccg catctgcgct gggcttggct tcgccgagca
     6361 tcagcgcgtc agtgccgtgc atcacgacac cgacaacctg cacatccata tcgccatcaa
     6421 caagattcac ccgacccgaa acaccatcca tgagccgtat cgggcctacc gcgccctcgc
     6481 tgacctctgc gcgacgctcg aacgggacta cgggcttgag cgtgacaatc acgaaacgcg
     6541 gcagcgcgtt tccgagaacc g
//

