LOCUS       Ple88\(pEMS1375)       18665 bp    DNA     circular     11-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|545679050|
COMMENT     VNTDBDATE|545679050|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple88 (pEMS1375)|
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.3883 -17.3191 40.0378 40.0378 0.01 10 -1 18665 18665 1 0 0) 
COMMENT        #139=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -179 100) 
COMMENT              (CObjectList 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple88 (pEMS1375)" "@N" 1 0 
COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
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COMMENT               #144=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1092 100) 
COMMENT                     (CObjectList 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -12.2322 -6.32595 0 -10 3.02214 1.17389 #146#) 
COMMENT                             #148=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #108# 674 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.479 1.9 0.082322 
COMMENT                                   1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5mutant)" "@N" 1 12.9903 12.2333 0 
COMMENT                                   -10 7.91752 1.17389 #148#) 
COMMENT                             #150=(CScratch 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #108# 828 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.46757 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 14.105 10.1913 0 -10 
COMMENT                                   7.99245 1.17389 #150#) 
COMMENT                             #152=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    5.46522 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   11.7606 7.84951 0 -10 2.67247 1.17389 #152#) 
COMMENT                             #154=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.22323 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 17.5703 
COMMENT                                   4.45874 0 -10 10.0405 1.17389 #154#) 
COMMENT                             #156=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #108# 30779968 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.21113 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   14.8188 2.34176 0 -10 1.99811 1.17389 #156#) 
COMMENT                             #158=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   14.9774 0.149858 0 -10 2.72243 1.17389 #158#) 
COMMENT                             #160=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #108# 30170736 
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COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 17.2503 
COMMENT                                   -1.74233 0 -10 11.0645 1.17389 #160#) 
COMMENT                             #162=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.2268 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Ple88 (GFAP-A)" 
COMMENT                                   "@N" 1 9.41963 14.9937 0 -10 7.11827 1.17389 
COMMENT                                   #162#) 
COMMENT                             #164=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.670712 5.48169 
COMMENT                                    6.2268 1 0.082322) 0.8 1.8 0) 
COMMENT                             #165=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #100# 47075536 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Region LongProm" "@N" 1 -3.25148 14.1005 0 
COMMENT                                   -10 8.51695 1.17389 #164#)) (CObjectList)) 
COMMENT                      #166=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -953 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #116# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.00201656 0.412723 1 0.082322) 0.8 1.8 1) 
COMMENT                             #168=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #100# 22068408 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -4.54705 12.1963 0 -10 2.87229 1.17389 #167#) 
COMMENT                             #169=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #116# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.07134 2.55061 1 0.082322) 0.8 1.8 0) 
COMMENT                             #170=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #100# 21513372 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -9.76789 -9.51089 0 -10 2.87229 1.17389 #169#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #171=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -534 100) 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.3721 
COMMENT                                   3.74044 0 -10 10.4901 1.17389 #172#) 
COMMENT                             #174=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 5.58824 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #174#) 
COMMENT                             #176=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #104# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.6598 
COMMENT                                   -4.93329 0 -10 14.9609 1.17389 #176#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #178=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -185 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #179=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -674 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #180=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -604 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #181=(AnlzFontBackColorItem 138 2354 33554687))) (CObList) 
COMMENT       1699618931 (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     12528..16339
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(17438..18659)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     11077..12502
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     7436..11081
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3482..7435
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    12509..12526
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    2364..2397
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    2398..2408
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    2405..3124
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    3125..3160
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    3161..3233
                     /vntifkey="21"
                     /label=NLS
     misc_feature    3239..3272
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    3426..3476
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..2355
                     /vntifkey="21"
                     /label=Ple88\(GFAP-A)
                     /note="1306"
     misc_feature    139..2355
                     /vntifkey="21"
                     /label=Region\LongProm
BASE COUNT     4932 a      4296 c      4329 g      5108 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccca gatgtgacta gagcctaagg agctcccacc tccctctctg 
      181 tgctgggact cacagaggga gacctcagga ggcagtctgt ccatcacatg tccaaatgca 
      241 gagcataccc tgggctgggc gcagtggcgc acaactgtaa ttccagcact ttgggaggct 
      301 gatgtggaag gatcacttga gcccagaagt tctagaccag cctgggcaac atggcaagac 
      361 cctatctcta caaaaaaagt taaaaaatca gccacgtgtg gtgacacaca cctgtagtcc 
      421 cagctattca ggaggctgag gtgaggggat cacttaaggc tgggaggttg aggctgcagt 
      481 gagtcgtggt tgcgccactg cactccagcc tgggcaacag tgagaccctg tctcaaaaga 
      541 caaaaaaaaa aaaaaaagaa catatcctgg tgtggagtag gggacgctgc tctgacagag 
      601 gctcgggggc ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct 
      661 gcaagcagac ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt 
      721 gaatgactca ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc 
      781 gcaccccagc ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc 
      841 accccagcct gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc 
      901 cttgctgtgt ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa 
      961 aggggatcag gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt 
     1021 ctgcttccca gaagtccaag gacacaaatg ggtgagggga ctgggcaggg ttctgaccct 
     1081 gtgggaccag agtggagggc gtagatggac ctgaagtctc cagggacaac agggcccagg 
     1141 tctcaggctc ctagttgggc ccagtggctc cagcgtttcc aaacccatcc atccccagag 
     1201 gttcttccca tctctccagg ctgatgtgtg ggaactcgag gaaataaatc tccagtggga 
     1261 gacggagggg tggccaggga aacggggcgc tgcaggaata aagacgagcc agcacagcca 
     1321 gctcatgtgt aacggctttg tggagctgtc aaggcctggt ctctgggaga gaagcacagg 
     1381 gaggccagac aaggaagggg tgacctggag ggacagatcc aggggctaaa gtcctgataa 
     1441 ggcaagagag tgccggcccc ctcttgccct atcaggacct ccactgccac atagaggcca 
     1501 tgattgaccc ttagacaaag ggctggtgtc caatcccagc ccccagcccc agaactccag 
     1561 ggaatgaatg ggcagagagc aggaatgtgg gacatctgtg ttcaagggaa ggactccagg 
     1621 agtctgctgg gaatgaggcc tagtaggaaa tgaggtggcc cttgagggta cagaacaggt 
     1681 tcattcttcg ccaaattccc agcaccttgc aggcacttac agctgagtga gataatgcct 
     1741 gggttatgaa atcaaaaagt tggaaagcag gtcagaggtc atctggtaca gcccttcctt 
     1801 cccttttttt tttttgtgag acaaggtctc tctctgttgc ccaggctgga gtggcgcaaa 
     1861 cacagctcac tgcagcctca acctactggg ctcaagcaat cctccagcct cagcctccca 
     1921 aagtgctggg attacaagca tgagccaccc cactcagccc tttccttcct ttttaattga 
     1981 tgcataataa ttgtaagtat tcatcatggt ccaaccaacc ctttcttgac ccaccttcct 
     2041 agagagaggg tcctcttgct tcagcggtca gggccccaga cccatggtct ggctccaggt 
     2101 accacctgcc tcatgcagga gttggcgtgc ccaggaagct ctgcctctgg gcacagtgac 
     2161 ctcagtgggg tgaggggagc tctccccata gctgggctgc ggcccaaccc caccccctca 
     2221 ggctatgcca gggggtgttg ccaggggcac ccgggcatcg ccagtctagc ccactccttc 
     2281 ataaagccct cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc 
     2341 acctccgctg ctcgcggcgc gccgaagttc ctattcttca aaaggtatag gaacttccgc 
     2401 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 
     2461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 
     2521 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 
     2581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 
     2641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 
     2701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 
     2761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 
     2821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 
     2881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 
     2941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 
     3001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 
     3061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 
     3121 gttatttctg aactgctgcc cgggctgctg catggaaccg gatccaaaaa agaagagaaa 
     3181 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taatataaga 
     3241 agttcctatt ctctagaaag tataggaact tcagcggccg cgactctaga tcataatcag 
     3301 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 
     3361 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 
     3421 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcgaat 
     3481 tctcgtaaaa ctcttcatgg cagtagttat tattctctct ctctctcttt ttcttttttc 
     3541 ttgagacagg atatttctct gttgcccagg ctggagtgca gtggcacagt cttggctcac 
     3601 tgcagcctgg acctcctggg ctcaagccat cctcccacct cagcctccca agtagctggg 
     3661 gctacaggca catggccacc aggcccagat aatttttcat ttttgtagag actgagtctc 
     3721 accatgttac ccaggtttat tattctcatt ttttagatga agagactgag gtccagagaa 
     3781 gctcaatgac ttgcctagtt ttacaaatct cctgccatca catacccctc agcgtcctta 
     3841 ataagaggga ggccaccaac tatgtgctgg gcactgtggt ggatgctgga gctatagggt 
     3901 tgagtatata agaaatggtg ttgctggagc aactgttgct tgcttacctg acctatctga 
     3961 gaattaatta gcaggggaac atatttttgt tttcagattc aatataagaa cttgtgtggg 
     4021 caaaaataaa gatcagtagt aataacagta gttcccattt gctgactgta ctgtcctaag 
     4081 tgcatatata tatacataca cacacgcata cctatactcc tctaatactc aaaatgatcc 
     4141 tgtttatgta ttgttaatat gctcatttta tttttaaatt tttatttatt tttattttta 
     4201 tttatttttg agacggagtc tcattctgtc gcggaggctg aagtgcagtg gtgcgatctc 
     4261 agctcagtgc gacctccgcc tcccgggttc aagtgattct cctgcctcag cctccggact 
     4321 agctgggatt acaggcgccc gcctccacgc ccagctaatt tttgtatttt tagtagagat 
     4381 ggggtttcgc catgttggcc aggctggtct cgtactcctg accttgagtg atccacctgc 
     4441 ctcggcctcc caaagtgctg ggattacagg catgagccac cgcgccgggc taatatgctc 
     4501 attttagtga ggcaaaaata gaggctcaga gtctgatttg tacaaaacta cagagcagtt 
     4561 aagtgtcctc tcagatgtgt accctgatct gggtgactct aggactctag gtctcaactg 
     4621 ttacaaccag ttaagggttt ggggaagcac tgggccaaga gtcaggaaaa tggaagccac 
     4681 aggtagtgca aggtcttggg aatgggacgt ctggtccaag gattcacgcg atgactggaa 
     4741 cccgaagagc cggggcccgg tttacggccg ccatgaagca acgcgcgccg gtaggtttgg 
     4801 gaatcaggga gccctctgaa taggagactg agttgggagg gaaaggggct tcgctggggg 
     4861 agcctcggct tcttctggga gaaaattccc acggctacct agtgagcctg caaactggta 
     4921 ggcgccggcg taggcgcgcg ggcggggccg ggggcggggc ctgcggggcg tggcggggcg 
     4981 ggcagagggc ggggcctgct tctcctcagc ttcaggcggc tgcgacgagc cctcaggcga 
     5041 acctctcggc tttcccgcgc ggcgccgcct cttgctgcgc ctccgcctcc tcctctgctc 
     5101 cgccaccggc ttcctcctcc tgagcagtca gcccgcgcgc cggccggctc cgttatggcg 
     5161 acccgcagcc ctggcgtcgt ggtgagcagc tcggcctgcc ggccctggcc ggttcaggcc 
     5221 cacgcggcag gtggcggccg ggccctgagg cgcgggatcg atccatgaca cccccccgcc 
     5281 ccccatatct cactataatt cactgtcaaa tggcttttat gatttggagt ttggggatac 
     5341 ttttaaagac agggtcttgg tagctcctag atggcttggg attcacttag taacccttgc 
     5401 tggcctaaaa ttcaggaact tctacctcat cttcccaagt actgggttag tagggatgtc 
     5461 cagcatattt agcttgcttt aatgattttg ctgtactttt ttctgtgtgt acaggtgcat 
     5521 gtatatttat aaaactaaca taatatattt tacccccaaa gtaccataac cctaattaat 
     5581 gctattaaat gttttgtatg ctgggctctg ggcatactaa tataaccaat attatacaat 
     5641 gcaaaacaat tttggaatat cttctgtaaa attatcttat tagtaagttg taaccatctc 
     5701 agacttctaa gtttgagttt gtaaaggatc agtttttttt ggtgacctac acacacacac 
     5761 acacacacac acacacacac acacacaggg gggtggattc tgagaattta gagaatttag 
     5821 atggttaata ccagttttta tttttattct gtgtgatatt tatttattaa gatttattta 
     5881 tttattacat gtaggtacac tgtatctgtc ttcagacaca ccagaagagg gcatcagatc 
     5941 tcgttacaga tggttgtgag tcaccatgtg gttgctggga tttgaactca ggacctctgg 
     6001 aagaacagtc agtgctctta accgttgagc catctcacca gccccgtgat attttattta 
     6061 acttactaga atatactgtg ttaaaacata ttttagggga ttggggagat ggcccattct 
     6121 gtaaagtgtc tgccatgtca tcttcaggac ctgcgtttgt aaccccagca tggagaagac 
     6181 agagacaggg agacccctgg gcttcctggc aagctagact agcaaatttg gttaactagg 
     6241 ttcaatgaga gactcagtct caaaacataa gaagtgaatt ttttagaccc aatttcaatc 
     6301 tctgaactcc atacgtatgc acatgtacct gcatataaat atatatacac acccataaac 
     6361 acatgcacac acaaaaaatg gttcaaaagt tcttaaaatg tataagagta gaaagtgtag 
     6421 agccgggagt ggtggcgcac gcctttaatc ccagcacttg ggagcagagg caggtggatt 
     6481 tctgagttcg aggccagcct ggtctacaga gtgagttcca ggacagccag agctatacag 
     6541 agaaatcctg cctcaaaaaa ccaaaaataa agtgtatata ctaatctttc atgcctaaaa 
     6601 tatttatttt tgccacattt ttcatacctc tttaatattt ttctttttct ctttgtgctt 
     6661 caaaggaaat tttgtgtcat gttttacttt tcattcttga ctctattcag cagtaagacg 
     6721 cagcatggct ttcttagaga agtataatct attacaaaac ggtaacattt aactgctcta 
     6781 cataccctct tgtagctaaa ttcctctgtt aaactaagac ttattatcta aacgtaatta 
     6841 aatgttgata tcttataatg ctccactttg aaacagctgt ctttcttttg cagattagcg 
     6901 atgatgaacc aggttatgac ctagatttgt tttgtatacc taatcattat gccgaggatt 
     6961 tggaaaaagt gtttattcct catggactga ttatggacag gttagtaaga cctcgattga 
     7021 agttattttt agagggtgtg tgtgtggtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 
     7081 gtgtgtaaga gagagaggag aggcatgtgt gatatatatg tgaagtgtat tgcatgtaca 
     7141 tggaatccag agaaggacat taggtgtcct actctgttgc tctcttattc ccttgaatca 
     7201 gtgtctcttg ctgaacctgg ggcatacata ctgatttggc taggctgcct ggctagcaag 
     7261 acccagggat ctgcttgtct ctgcccccca gtgctgacgt tacaagcagg tgcaaccaca 
     7321 tctggttttt atatgggtac tgggggatct gaacttaggg cctgatgcgt gcccaagtgc 
     7381 tctcacccgc caagccacct ctccagccaa agggtttttt ttcttgtatt aattctaact 
     7441 tagcaactgt tgagtagttg atgtttgaat ataagctaag ccaacttatt agaataaatg 
     7501 ttttctattt tttacttgta tgtgtgagtg cctgcatgta tgtatgtgaa gcatatgtat 
     7561 tccaggatgt cagaattgtg atggattccc tggaactgga gttacagacc attgtgaacc 
     7621 actgtgtatg ggtgctggga actgaacttt aggtcctctg caaaaacagt aaggatctta 
     7681 actgataaac tatctttcta gccctaaggc tttctacttt taaagttcgt ttctctttct 
     7741 ctctctctct ctctctctct ctctctctct ccctctccct ccctcctcct cctcctcctc 
     7801 tttcccccct ctcgcctcct ctttctctct cccctccctc tctctctctc acacacacac 
     7861 acagacagag agaggagaga gaaaggaagc cttactaatt aattacctag ttttagacat 
     7921 aactagtggg aatccatagt aagtaaatca acattcttga agagaggtta gaagaattgg 
     7981 ctctcgttgt ccatcgaggc tgtataagat tattcttctg ttttctcagc ttaccataca 
     8041 tattttctac gtagacaata ccaattcaaa tacactgtac gtaaagtata tgcgcacaca 
     8101 cacacacaca cacacacaca cacacacaca agttacatat tgtcatatag tactaaaatt 
     8161 tatggacttt ttcatattaa tacatctaga gctatcatgt tttttatctt ttaaaaatta 
     8221 ctttacgggg ctacagagat gtctcagcag ttaggagcac tgactgctct tccagaggtc 
     8281 ctgagttcaa ttcccagcaa ccacatggtg gctcacaacc atctgtaatg ggatctgatc 
     8341 cactcttatg gtgtgtctga agacagtgac agtgtactca tatacatgaa ataaatcttt 
     8401 aaaaaattac tttaccatca gggtatatat atatatatat atgtgtgtgt gtgtgtgtgt 
     8461 gtgtatgaag tatttgctat ttgagagggt ctcactgttt agctcagtct ggcttggaac 
     8521 gttctgtata gcccagactg gcctcaactc agcagtcccc ctgaaaggct ctcatatgtg 
     8581 gagattacag gtatgtgcta ctgtgcccag ccctacatgt tactttgttt ttgaaatagg 
     8641 gtatcaaata gcttagactg gcttctaagt cagtgtgtag ctaaggatgg tctgagcttc 
     8701 tgaacctgct acttcaactc ctggtgtgcc atcatggcca gtttgcacag ttgtgagagt 
     8761 agaactcagg acctcctatg tgttactaga catgttctct accagctgag ctacatccca 
     8821 attcgcctac atttactttt attttttaaa agatttattt atgtatacag cactctgcct 
     8881 gcatgtacat ctgcatgcca gaagagggca ccagatctca tcatacatag ttgtgagcca 
     8941 ccatgtggtt gctggggatt gaactcaggg cctctggaag agcagtcagt gctcttaacc 
     9001 tctgagccat ctctgtagcc ccctacattt attttaaaga ttataggcta ttctattcat 
     9061 agatgtgcca taactgattt aaccagatct tcattatttt gtacttagta gagtatatta 
     9121 gagggaatga atcttaaata cacattttga cacttgctca gctttagaca agtaaaaatc 
     9181 actggtcaag gaaatggtgc ttagtggatg cttctgaact gtcaaattgt cctccaacaa 
     9241 gttacatcat tttatacttc caacgttatg tgagagttca tttttcttta ggctcttcta 
     9301 gtattttata tttgagaaat aaaatttaac caaaatttgc caatctagta agcttgtttt 
     9361 tatttgtatc tttaaatttt atgatagatg ttatagtgta ctctcctctc ccctcccctc 
     9421 ccctctcctc ctcagcagga tcttgttatg tagccctaag ggatctaata cttactctgt 
     9481 agtccaggct gtccttcagc tcatgagaca caccatcatg cttggcatgt ttcttgttct 
     9541 gaaaggtatc tggttttaat tttggttgag ctggtgtgtt ggtgtatatc tgtaatctca 
     9601 gcacaggcaa gtagagacaa ggattatgat tacagtccag cctgggctat ttagagggat 
     9661 tctctttaat ctttcctcat gccccaaaat cttacctttg gtatatgaaa aatagtctcc 
     9721 acttctgcaa aatattgctt tatgaagtaa gaattaattc ccttcataga gacaaggaat 
     9781 gtgtcctgta aaagtttaat gtgtaagaag tatttgttat aaaagataaa tattcagaat 
     9841 cttcttttta attcctgatt ttatttctat aggactgaaa gacttgctcg agatgtcatg 
     9901 aaggagatgg gaggccatca cattgtggcc ctctgtgtgc tcaagggggg ctataagttc 
     9961 tttgctgacc tgctggatta cattaaagca ctgaatagaa atagtgatag atccattcct 
    10021 atgactgtag attttatcag actgaagagc tactgtgtaa gtataattaa cttataatta 
    10081 aaaaaatagg gccattctag ttttatctat atttttttta aacttgtgca aactatgcta 
    10141 cataatcaac ttacttgatt atgtaatata tatagtaata gttggaatta ctattattac 
    10201 atatttattt ttctgtgctg gggtagaacc cagggaacta agtactgtaa taaccgagct 
    10261 tcattcatcc tagtcatttt taattttttt tttttttttt gttttttcga gacaaggttt 
    10321 ctctgtgtag ccctggctgt cctggaactc actctgtaga ccaggctggc ctcgaactca 
    10381 gaaatctgcc tgcctctgcc tcccagtgct gagattaatg acgtgcacca ccactgcctg 
    10441 gcattttttt aaagttttaa tttgagaccc tttctcacta acttgctcac actatcctcg 
    10501 aactcattac gtgggctggt ctagaaattt cagtcaactt gcctcagctt ctcaagtagc 
    10561 tgggattaca ggcaggtgct accacttctg gccatgatca ttgttgttgt tgctattatt 
    10621 attattatta tttcactgat acttcatatc acaacacaga atagtaactg tagttcatga 
    10681 ccaacaaaaa agttgtacag tttctctgtt gtcttatttt gatctggatt ttgatttgtt 
    10741 tcatggcact gggcaggcta atctcaaact gcatggctca aatagtcctt ctgtcttaat 
    10801 ctcccatgtt ctggaagtat aggtatcact acacctagct gtattttcat attttaaaaa 
    10861 aaacgcctaa gaattgctat tgaatgaggc ggagagatgg ctcagcagtc acaagtactt 
    10921 gtagaggact ggggtttggt tactagcacc tcaaagatgg cttacaatta tctgtagctc 
    10981 caattccaga ggatctgtgt ggtaaagctg catgtatatg ggcatgcagg taaaactgct 
    11041 aaacatataa aaatagctca gtcttaagag aattggtcga ctttttcgcc ctttgacgtt 
    11101 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
    11161 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
    11221 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
    11281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
    11341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
    11401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
    11461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
    11521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
    11581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
    11641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
    11701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
    11761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
    11821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
    11881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
    11941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
    12001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
    12061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
    12121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
    12181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
    12241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
    12301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
    12361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
    12421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
    12481 acaaaaaaac caccgctacc aggtcgagta gggataacag ggtaatggat cctcgttggg 
    12541 cacctgcact catgtacgca ggcccacata tagatgtaca cacgcatatc attcaaaata 
    12601 gaaattattt atacaagtag tgcgttggag agctggctca ggagttgtta atgcttgcta 
    12661 cccttgcaaa ggacccaagt tcaatttcca gcacctgtgt tgggcatctc acaaccacat 
    12721 gtaaatctag tttcagggga tttcacccct cttctgaact cttcaggcac ttatacccac 
    12781 aaatggacat ctccacatag agacacatac ttttaaaaaa gtaatctagg gtctagagaa 
    12841 atggttatag accacggact gggatcagtt cccagaagcc acatcctgtg gctcacaatt 
    12901 gcctgtgaat caagttctag atctggtggc ctcgtctagc ctgcagtcat caacaaacac 
    12961 atgcacacat ccatacacac ataatttaaa agaaagcgaa agtaacctat aatgctaaac 
    13021 aaagcacatt ctcagtgtag actgcagtgg tgatgaacct tgaaaatata ttaatagaag 
    13081 ccagacacgt aagtcacaaa tggtataact caatttatat gaaattccca gagagtccaa 
    13141 agttaaaaaa aacaaaaagg cagattattg gttgtcagta gctacagaga tgggacattg 
    13201 aagatattaa taggtatagg gttgaggatt catggccttt ccctcttcca tgttagcatg 
    13261 tctattgttg ttaaccttat tcaactcatg ttagggcaat catattgatg agacttaatg 
    13321 agtatagctt gacattccta gaagacagag tctcacatca gactctctga tcctctgact 
    13381 cttagaatct ttcaacaccc tcttctgaaa tgatctctga gcctttggtt gtgactgaat 
    13441 tatgagaaag aattttatgt tgaatgaatt ttacttcagc atttttacaa aactgaagct 
    13501 ggggatggcg tttcatgcgt gcagtcccta ccatttgcct ataagcctgg aggaggcaga 
    13561 agtagaatta ggcttttcag ggacatcttc agctgcatca tgagcttgag gacaacttga 
    13621 gctacataag attcccatcc ccacccccac acacacacac acatctagta tgtacctagt 
    13681 atgcagtctt ttcatatgtt taaaatgtag aaaggtatat gtgaagtctc ccttttcccc 
    13741 acaacttagt ccctccaaag tattgcatca gtttccttgt acatgatttt atgcatatat 
    13801 aagatacact atttctctct ctctctctct ccttccctcc ttccctccaa cctctgggta 
    13861 tgagttgagg gttatgtatg tttcaagttg taataagaat tgccactcaa acatttcatc 
    13921 ccattttaag ttaagtttgt ctgattttcc actttagaag ggcagccatg atggctcatg 
    13981 cctataatcc catcctagca cttgtaagcc taatacataa gactggagga ttgtcatcag 
    14041 cttgaggcta gcctggtcta cctaatgagt tccagccagc caagagtaga atataagacc 
    14101 ctgtctccaa aaaatgtacc aaaggggctg gaggggtaac tcagtggtta agaacagtag 
    14161 ctgctcttcc agaggacctg ggttcaattc ccagcaccca catggcagct tgcaactatc 
    14221 tgtaactgtc cagctttggg atatccgaca ccattatacc aattacataa aatgaaaaaa 
    14281 ttgtaaaatg tgctaaaatg agtggggagg gggagcatgg tgtggttctg tgggcttggg 
    14341 attttgccat gaatccatga ctatatctca gcaatatgtg gattatttct ggaaacctgt 
    14401 ggggcatggg tgtatgggaa gaggggagag agagagagcc cgcatccagc cagagttcct 
    14461 gtgctctggg caggcggacg tgggaggact gctggacgat ttccacttgg ccctgggtgg 
    14521 gcatctggct gtgtgaagcc actgacccca ctcagcgggg ggtagataag gggcatttcc 
    14581 cataccaggt tctcagactc cagcattcca cgctggatac agcagaagag ctgagaacag 
    14641 aataggggcc ccagggagac actctcgtcc cagagataaa gaaggcagag ggagagagat 
    14701 tcctacgcag gcgagaggcc ttagtctggg ccttgatgaa cagagacggt ctatggtttt 
    14761 agagctttat tgtagaaagg cagggggaaa gagagaaggt agaaagagag agaccagcca 
    14821 tggccaagag gagagaaggg gggaaaggag agagagaaga aaggctagag agtaagaaag 
    14881 gttaaagaga cagaagtaaa agcttagagt aagagagaga gagagagcaa gagggagagt 
    14941 aagaaaagta aaagcttaag agagcgagga ggggccagcc cctctaatag tgggcttgtt 
    15001 atcttgttgt tgctaggtaa ctgtgaagcg tctagcctga aggccagaag cttgggacat 
    15061 tgtctatgtg actgaaagcc atgagcctct actgtggggg ctgtggaggt ggtaacttgg 
    15121 acaggagcca ggggttccag gagacatgag ggaatgcctt ctgtcccatg taggtaggaa 
    15181 ttaccaccac cggctttcac cgtagttcac tggagactgt ttgtacatag cccattgccc 
    15241 cacagaaacc aggcactcac tgtttcattt gcataagaat tagaaggctt tattttggct 
    15301 gggattggtg acttgcccca gaaattccag cttttgggaa gccgaatcct ggagaaccag 
    15361 gagttcaagg tcatcctcag gtacctcatt tgttcaagac cagcctgtgc taccatgaga 
    15421 ccttgtccct actcccctca accccaaaac acgctgtttt cattttatag gttgtactaa 
    15481 ccatggagga tggtcatttg cctttctctt gatttcagaa gcgtagcgcg ggctttgcac 
    15541 catgatctag tgtttccaga taggcttgcc tctccacagc caggcttatc ctgcttcagt 
    15601 cccatgtttg gcaagggcaa catatgactg aagaacttgc atagccttat ggtgtatcat 
    15661 gactcttagc cccagtttat aactgtctaa cagctggtgg cttttgtgaa aaaggaaagc 
    15721 atcatttgaa cttttaactt ttcagctgtt tgttttgact ttgcataaat actgagacta 
    15781 gctaggaaat gagcttttgt ttcatttgct aaaaatgatt tttttttcat gaggcaattt 
    15841 caagcaattt gaatttagca atctctactt gtctgaaaag aagttattaa ttaaaaaaaa 
    15901 acaaacaaac aggaacctat tatgctggct agtcacatga aagaatccaa atgaacacag 
    15961 tcaaggctga atcaacacat gaaattattt tctaattccc caaattacat ttatgctaat 
    16021 tagtatgtag taaaacattt taattgtttc caatgttaaa cattacagaa ataattttga 
    16081 tgttttccta ataacccagc ctttgatcat gtttgcattt atttgattgt tgtcagttta 
    16141 ttaaataatt aagtaagagc aacaagataa aatgacttca tgagagtcct tttctgtaac 
    16201 ttagaacgtc agtagtcata ggaactgcgg tcgttccctt acctttcaga agtaggctaa 
    16261 agagttgaac gcaaaggtgg tttttcgaat cttcctgatc cttaagcccc cactaggttt 
    16321 atattctata tttgatatcg gattcgaacc cctcgagctt aggatccgga acccttaata 
    16381 taactaagtt attaggtccc tcgaagaggt tcactagtac tggccattgc ggccgcttcg 
    16441 aggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
    16501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
    16561 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 
    16621 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 
    16681 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 
    16741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
    16801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
    16861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
    16921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
    16981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
    17041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
    17101 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
    17161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
    17221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
    17281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
    17341 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
    17401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
    17461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
    17521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
    17581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
    17641 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
    17701 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
    17761 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
    17821 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
    17881 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
    17941 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg 
    18001 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
    18061 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
    18121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
    18181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
    18241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 
    18301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
    18361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
    18421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
    18481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
    18541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
    18601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
    18661 gacgg 
//
