LOCUS       pEMS1983        7170 bp    DNA     circular      9-OCT-2012
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|628102969|
COMMENT     VNTDBDATE|639492565|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1983|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMS1983" 0 7170 
COMMENT       (CDBMol 0 0 1 4700 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 8 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 100 0 1 0 
COMMENT        (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AscI" "AvrII" "FseI" "MluI" "EcoRI" "SmaI" "PvuI" "AsiSI") 
COMMENT        (CStringList "atg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 1 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7170 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 8 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2695 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 198 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 207 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 192 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SmaI" "cccggg" 4 0 6 32 0 43 0 2612 0 3661 0 
COMMENT            4319 0 4330 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 3929 0 4497 0 5542 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 3929 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "Amp-R" 4 0 0 5123 5980 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5123..5980") 
COMMENT        #9=(CFSignal (CObList) "Origin" 21 0 0 6154 6742 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6154..6742") 
COMMENT        #10=(CFSignal (CObList) "additional AAV sequences" 21 0 0 4185 4230 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4185..4230") 
COMMENT        #11=(CFSignal (CObList) "5' ITR" 34 0 0 1 168 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..168") 
COMMENT        #12=(CFSignal (CObList) "3' ITR" 34 0 1 4193 4360 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4193..4360)") 
COMMENT        #13=(CFSignal (CObList) "iCre" 4 0 0 2886 3917 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2886..3917") 
COMMENT        #14=(CFSignal (CObList) "MCS 5' end" 21 0 0 197 208 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "197..208") 
COMMENT        #15=(CFSignal (CObList) "SV40" 25 0 0 3932 4152 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3932..4152") 
COMMENT        #16=(CFSignal (CObList) "Intron cassette" 15 0 0 2701 2873 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2701..2873") 
COMMENT        #17=(CFSignal (CObList) "Ple253" 21 0 0 209 2692 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "209..2692") 
COMMENT        #18=(CFSignal (CObList) "oEMS5931" 27 0 1 276 296 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "oEMS5913" 27 0 1 2902 2922 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "aggggttcttcacgtctgatatctg" 63 243 25 1 100 100 100 
COMMENT              56.6396 48 -182.4 -472.4 -39.774 -10.612) 171 101 267 167 76.7292 
COMMENT             50.8982 55.7561) 
COMMENT        #21=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "tgaggggttcttcacgtctgatatc" 63 245 25 1 100 100 100 
COMMENT              57.353 48 -180.2 -465.1 -39.7505 -10.612) 171 101 269 169 76.7727 
COMMENT             50.8876 55.7865) 
COMMENT        #22=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "ctgaggggttcttcacgtctgatat" 63 246 25 1 100 100 100 
COMMENT              56.6396 48 -182.4 -472.4 -39.774 -10.612) 171 101 270 170 76.9147 
COMMENT             51.1765 55.8859) 
COMMENT        #23=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "ctgaggggttcttcacgtctgata" 63 247 24 1 100 100 100 
COMMENT              56.2319 50 -173.8 -448.5 -38.2997 -10.4798) 171 101 270 170 
COMMENT             76.9147 51.1765 55.8098) 
COMMENT        #24=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "ctgaggggttcttcacgtctgat" 63 248 23 1 100 100 100 
COMMENT              56.0888 52.1739 -167.8 -431.6 -37.3385 -13.1299) 171 101 270 170 
COMMENT             76.9147 51.1765 55.7669) 
COMMENT        #25=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "actgaggggttcttcacgtctga" 63 249 23 1 100 100 100 
COMMENT              56.5457 52.1739 -165.7 -425 -37.2063 -12.9977) 171 101 271 171 
COMMENT             76.8152 50.8772 55.8163) 
COMMENT        #26=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "ttactgaggggttcttcacgtctg" 63 250 24 1 100 100 100 
COMMENT              56.2944 50 -175.2 -452.4 -38.537 -13.3766) 171 101 273 173 76.6197 
COMMENT             50.289 55.6221) 
COMMENT        #27=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "tgcaggtgtaggaagttactgaggg" 63 264 25 1 100 100 100 
COMMENT              57.6086 52 -184.7 -477.5 -40.5534 -14.2062) 171 101 288 188 
COMMENT             77.0306 50.5319 55.967) 
COMMENT        #28=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "cccaatgtgcaggtgtaggaagt" 63 273 23 1 100 100 100 
COMMENT              56.7965 52.1739 -171.9 -442.3 -38.2483 -12.0888) 171 101 295 195 
COMMENT             77.2567 50.7692 56.1254) 
COMMENT        #29=(CPrimers (CStringList) (CStringList) 
COMMENT             (CPrimer 0 "gagtggccaactccatcactagg" 63 101 23 0 100 100 100 
COMMENT              56.4854 56.5217 -169.5 -435.9 -37.7564 -12.0983) 
COMMENT             (CPrimer 0 "gcccaatgtgcaggtgtagga" 63 276 21 1 100 100 100 57.1154 
COMMENT              57.1429 -159.6 -406.9 -36.5028 -11.8418) 171 101 296 196 77.3774 
COMMENT             51.0204 56.2098)) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #30=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #31=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEMS1983" 2) 5 "" 
COMMENT                   0 4) 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pEMS1983'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: User" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/05/12 05:02PM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/09/12 12:49PM" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7170 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-SEP-2012" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "User" 1))) 
COMMENT                   "Original Author") 
COMMENT              #45=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2886 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2886  End: 3917" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2886..3917" 1))) "iCre") 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5123 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5123  End: 5980" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5123..5980" 1))) "Amp-R"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2701 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2701  End: 2873" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2701..2873" 1))) "Intron cassette"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 197 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 197   End: 208 " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   197..208" 1))) "MCS 5' end") 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 209 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 209   End: 2692" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   209..2692" 1))) "Ple253") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4185 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4185  End: 4230" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4185..4230" 1))) 
COMMENT                                 "additional AAV sequences") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6154 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6154  End: 6742" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6154..6742" 1))) "Origin"))) 
COMMENT                          "Misc. Feature (4 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3932 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3932  End: 4152" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3932..4152" 1))) "SV40"))) 
COMMENT                          "PolyA Signal (1 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 276 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 276   End: 296  (Complementary)" 
COMMENT                                        1))) "oEMS5931") 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2902 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2902  End: 2922 (Complementary)" 
COMMENT                                        1))) "oEMS5913"))) "Primer (2 total)") 
COMMENT                     #89=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 34 (7 34 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 168 " 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..168" 1))) "5' ITR") 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4193 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4193  End: 4360 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4193..4360)" 1))) 
COMMENT                                 "3' ITR"))) "Repeat Region (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #98=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #99=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 59812336 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #100=(CGroupPar 
COMMENT                                   (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                          " N1: 2695 " 1))))) "AscI: 1 site") 2 
COMMENT                          6 "GGCGCGCC" "CCGCGCGG") 
COMMENT                     #102=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79333040 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #103=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 3929 " 1))))) "AsiSI: 1 site") 
COMMENT                           5 3 "GCGATCGC" "CGCTAGCG") 
COMMENT                     #105=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 59814064 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #106=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 198 " 1))))) "AvrII: 1 site") 1 
COMMENT                           5 "CCTAGG" "GGATCC") 
COMMENT                     #108=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79333744 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #109=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 192 " 1))))) "EcoRI: 1 site") 1 
COMMENT                           5 "GAATTC" "CTTAAG") 
COMMENT                     #111=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79332304 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #112=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 207 " 1))))) "FseI: 1 site") 6 
COMMENT                           2 "GGCCGGCC" "CCGGCCGG") 
COMMENT                     #114=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79332272 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "MluI: 0 sites") 1 5 "ACGCGT" 
COMMENT                           "TGCGCA") 
COMMENT                     #115=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79333232 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #116=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 3929 " 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 4497 " 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 5542 " 1))))) "PvuI: 3 sites") 
COMMENT                           4 2 "CGATCG" "GCTAGC") 
COMMENT                     #120=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 79332752 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #121=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 32 " 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 43 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2612 " 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 3661 " 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 4319 " 1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 6) 1 2 2 0 191) 
COMMENT                                           " N6: 4330 " 1))))) "SmaI: 6 sites") 
COMMENT                           3 3 "CCCGGG" "GGGCCC"))) 
COMMENT                   "Restriction/Methylation Map:  (no more than 8 sites)") 
COMMENT              #128=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 16 (0 0) 1 1 0 0 2104) 
COMMENT                     (CObjectList 
COMMENT                      #129=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #130=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "aggggttcttcacgtctgatatctg" 0 101 267 167 
COMMENT                                      0 0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #131=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 267" 
COMMENT                                     1) 
COMMENT                               #132=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.7 C  TaOpt: 55.8 C  GC: 50.9" 1) 
COMMENT                               #133=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #134=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #135=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #136=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #137=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #138=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #139=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #140=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #141=(COligo "" 
COMMENT                                              "AGGGGTTCTTCACGTCTGATATCTG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  AGGGGTTCTTCACGTCTGATATCTG" 1)) 
COMMENT                               #142=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #143=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 25  Tm: 56.6 C  GC: 48.0" 1) 
COMMENT                               #144=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -182.4 kcal/mol  dS: -472.4 cal/mol  dG: -39.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #145=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.2" 1) 
COMMENT                               #146=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 8.5" 1))) 
COMMENT                             "#1: Product of length 167 (rating: 171)")) 
COMMENT                      #147=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #148=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "tgaggggttcttcacgtctgatatc" 0 101 269 169 
COMMENT                                      0 0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #149=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 269" 
COMMENT                                     1) 
COMMENT                               #150=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.8 C  TaOpt: 55.8 C  GC: 50.9" 1) 
COMMENT                               #151=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #152=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #153=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #154=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #155=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #156=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #157=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #158=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #159=(COligo "" 
COMMENT                                              "TGAGGGGTTCTTCACGTCTGATATC" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  TGAGGGGTTCTTCACGTCTGATATC" 1)) 
COMMENT                               #160=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #161=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 25  Tm: 57.4 C  GC: 48.0" 1) 
COMMENT                               #162=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -180.2 kcal/mol  dS: -465.1 cal/mol  dG: -39.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #163=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.9" 1) 
COMMENT                               #164=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 8.5" 1))) 
COMMENT                             "#2: Product of length 169 (rating: 171)")) 
COMMENT                      #165=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #166=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "ctgaggggttcttcacgtctgatat" 0 101 270 170 
COMMENT                                      0 0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #167=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 270" 
COMMENT                                     1) 
COMMENT                               #168=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.9 C  TaOpt: 55.9 C  GC: 51.2" 1) 
COMMENT                               #169=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #170=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #171=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #172=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #173=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #174=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #175=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #176=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #177=(COligo "" 
COMMENT                                              "CTGAGGGGTTCTTCACGTCTGATAT" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CTGAGGGGTTCTTCACGTCTGATAT" 1)) 
COMMENT                               #178=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #179=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 25  Tm: 56.6 C  GC: 48.0" 1) 
COMMENT                               #180=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -182.4 kcal/mol  dS: -472.4 cal/mol  dG: -39.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #181=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.2" 1) 
COMMENT                               #182=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 8.5" 1))) 
COMMENT                             "#3: Product of length 170 (rating: 171)")) 
COMMENT                      #183=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #184=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "ctgaggggttcttcacgtctgata" 0 101 270 170 0 
COMMENT                                      0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #185=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 270" 
COMMENT                                     1) 
COMMENT                               #186=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.9 C  TaOpt: 55.8 C  GC: 51.2" 1) 
COMMENT                               #187=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #188=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #189=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #190=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #191=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #192=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #193=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #194=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #195=(COligo "" 
COMMENT                                              "CTGAGGGGTTCTTCACGTCTGATA" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CTGAGGGGTTCTTCACGTCTGATA" 1)) 
COMMENT                               #196=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #197=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 24  Tm: 56.2 C  GC: 50.0" 1) 
COMMENT                               #198=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -173.8 kcal/mol  dS: -448.5 cal/mol  dG: -38.3 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #199=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.3" 1) 
COMMENT                               #200=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 6.5" 1))) 
COMMENT                             "#4: Product of length 170 (rating: 171)")) 
COMMENT                      #201=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #202=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "ctgaggggttcttcacgtctgat" 0 101 270 170 0 
COMMENT                                      0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #203=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 270" 
COMMENT                                     1) 
COMMENT                               #204=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.9 C  TaOpt: 55.8 C  GC: 51.2" 1) 
COMMENT                               #205=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #206=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #207=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #208=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #209=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #210=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #211=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #212=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #213=(COligo "" 
COMMENT                                              "CTGAGGGGTTCTTCACGTCTGAT" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CTGAGGGGTTCTTCACGTCTGAT" 1)) 
COMMENT                               #214=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #215=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.1 C  GC: 52.2" 1) 
COMMENT                               #216=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -167.8 kcal/mol  dS: -431.6 cal/mol  dG: -37.3 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #217=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.4" 1) 
COMMENT                               #218=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 4.3" 1))) 
COMMENT                             "#5: Product of length 170 (rating: 171)")) 
COMMENT                      #219=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #220=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "actgaggggttcttcacgtctga" 0 101 271 171 0 
COMMENT                                      0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #221=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 271" 
COMMENT                                     1) 
COMMENT                               #222=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.8 C  TaOpt: 55.8 C  GC: 50.9" 1) 
COMMENT                               #223=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #224=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #225=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #226=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #227=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #228=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #229=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #230=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #231=(COligo "" 
COMMENT                                              "ACTGAGGGGTTCTTCACGTCTGA" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  ACTGAGGGGTTCTTCACGTCTGA" 1)) 
COMMENT                               #232=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #233=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 52.2" 1) 
COMMENT                               #234=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -165.7 kcal/mol  dS: -425.0 cal/mol  dG: -37.2 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #235=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.1" 1) 
COMMENT                               #236=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 4.3" 1))) 
COMMENT                             "#6: Product of length 171 (rating: 171)")) 
COMMENT                      #237=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #238=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "ttactgaggggttcttcacgtctg" 0 101 273 173 0 
COMMENT                                      0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #239=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 273" 
COMMENT                                     1) 
COMMENT                               #240=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.6 C  TaOpt: 55.6 C  GC: 50.3" 1) 
COMMENT                               #241=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #242=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #243=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #244=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #245=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #246=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #247=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #248=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #249=(COligo "" 
COMMENT                                              "TTACTGAGGGGTTCTTCACGTCTG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  TTACTGAGGGGTTCTTCACGTCTG" 1)) 
COMMENT                               #250=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #251=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 24  Tm: 56.3 C  GC: 50.0" 1) 
COMMENT                               #252=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -175.2 kcal/mol  dS: -452.4 cal/mol  dG: -38.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #253=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.2" 1) 
COMMENT                               #254=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 6.5" 1))) 
COMMENT                             "#7: Product of length 173 (rating: 171)")) 
COMMENT                      #255=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #256=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "tgcaggtgtaggaagttactgaggg" 0 101 288 188 
COMMENT                                      0 0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #257=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 288" 
COMMENT                                     1) 
COMMENT                               #258=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 77.0 C  TaOpt: 56.0 C  GC: 50.5" 1) 
COMMENT                               #259=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #260=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #261=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #262=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #263=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #264=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #265=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #266=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #267=(COligo "" 
COMMENT                                              "TGCAGGTGTAGGAAGTTACTGAGGG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  TGCAGGTGTAGGAAGTTACTGAGGG" 1)) 
COMMENT                               #268=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #269=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 25  Tm: 57.6 C  GC: 52.0" 1) 
COMMENT                               #270=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -184.7 kcal/mol  dS: -477.5 cal/mol  dG: -40.6 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #271=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 1.1" 1) 
COMMENT                               #272=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 4.5" 1))) 
COMMENT                             "#8: Product of length 188 (rating: 171)")) 
COMMENT                      #273=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #274=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "cccaatgtgcaggtgtaggaagt" 0 101 295 195 0 
COMMENT                                      0) 0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #275=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 295" 
COMMENT                                     1) 
COMMENT                               #276=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 77.3 C  TaOpt: 56.1 C  GC: 50.8" 1) 
COMMENT                               #277=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #278=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #279=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #280=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #281=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #282=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #283=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #284=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #285=(COligo "" 
COMMENT                                              "CCCAATGTGCAGGTGTAGGAAGT" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CCCAATGTGCAGGTGTAGGAAGT" 1)) 
COMMENT                               #286=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #287=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.8 C  GC: 52.2" 1) 
COMMENT                               #288=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -171.9 kcal/mol  dS: -442.3 cal/mol  dG: -38.2 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #289=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.3" 1) 
COMMENT                               #290=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 4.3" 1))) 
COMMENT                             "#9: Product of length 195 (rating: 171)")) 
COMMENT                      #291=(CPCRFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar 
COMMENT                              (CParagraph 0 
COMMENT                               (20 
COMMENT                                #292=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gagtggccaactccatcactagg" 0 
COMMENT                                      "gcccaatgtgcaggtgtagga" 0 101 296 196 0 0) 
COMMENT                                0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #293=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 101 to 296" 
COMMENT                                     1) 
COMMENT                               #294=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 77.4 C  TaOpt: 56.2 C  GC: 51.0" 1) 
COMMENT                               #295=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #296=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #297=(COligo "" 
COMMENT                                              "GAGTGGCCAACTCCATCACTAGG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GAGTGGCCAACTCCATCACTAGG" 1)) 
COMMENT                               #298=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #299=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 23  Tm: 56.5 C  GC: 56.5" 1) 
COMMENT                               #300=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -169.5 kcal/mol  dS: -435.9 cal/mol  dG: -37.8 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #301=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #302=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #303=(COligo "" "GCCCAATGTGCAGGTGTAGGA" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS1983" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCCAATGTGCAGGTGTAGGA" 1)) 
COMMENT                               #304=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #305=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 21  Tm: 57.1 C  GC: 57.1" 1) 
COMMENT                               #306=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -159.6 kcal/mol  dS: -406.9 cal/mol  dG: -36.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #307=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.6" 1) 
COMMENT                               #308=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.6" 1))) 
COMMENT                             "#10: Product of length 196 (rating: 171)")))) 
COMMENT                    "PCR Analysis")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #309=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #310=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #311=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #312=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #313=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #314=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #315=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #316=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #317=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #318=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #319=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #320=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #321=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #322=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #323=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #324=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #325=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #326=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #327=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #328=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #329=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #330=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #331=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #332=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #333=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #334=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #335=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #336=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #337=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #338=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #339=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #340=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #341=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #342=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #343=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #344=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #345=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #346=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #347=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #348=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 60.0567 60.0567 58.5 58.5 0.1 
COMMENT         -34817) 1 1 0 1 1 
COMMENT        (mapper: 20.9507 -19.0692 60.0567 60.0567 0.01 10 -1 7170 7170 1 0 0) 
COMMENT        #349=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -604 100) 
COMMENT              (CObjectList 
COMMENT               #350=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1382 100) 
COMMENT                     (CObjectList 
COMMENT                      #351=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #315# 80111876 0) 
COMMENT                             (LOGPEN 0 2 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27506 6.27306 1 0.0214037) 0.0874921) 
COMMENT                      #352=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #312# 2 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 87 33 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pEMS1983" "@N" 1 0 0.871165 0 
COMMENT                            -10 5.82783 1.46528 Nil) 
COMMENT                      #353=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #311# 194 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "7170 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 3.18033 0.932452 Nil)) (CObjectList)) 
COMMENT               #354=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1487 100) 
COMMENT                     (CObjectList 
COMMENT                      #355=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -2114 100) 
COMMENT                            (CObjectList 
COMMENT                             #356=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #313# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 1.04116 1.79184 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #357=(CLabel (CWidget 0 (0 0) 1 2 0 0 #310# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Amp-R" "@N" 1 
COMMENT                                   -13.3718 1.74233 0 -10 2.98052 1.11561 #356#) 
COMMENT                             #358=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #313# 895 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 2.84612 3.74904 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #359=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 1734828899 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "iCre" "@N" 1 
COMMENT                                   3.66556 -13.9386 0 -10 1.7983 1.11561 #358#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #360=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -1173 100) 
COMMENT                            (CObjectList 
COMMENT                             #361=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #331# 543716457 
COMMENT                                    100) (LOGPEN 0 0 16711680) 1 0.835356 
COMMENT                                   3.75954 3.9109) 
COMMENT                             #362=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 26946860 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Intron cassette" "@N" 1 11.5458 -10.454 0 
COMMENT                                   -10 6.2108 1.11561 #361#)) (CObjectList)) 
COMMENT                      #363=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1591 100) 
COMMENT                            (CObjectList 
COMMENT                             #364=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #314# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.374466 0.889795 
COMMENT                                    1 0.082322) 0.8 1.8 0) 
COMMENT                             #365=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 956 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Origin" "@N" 1 
COMMENT                                   -9.09241 10.454 0 -10 2.6808 1.11561 #364#) 
COMMENT                             #366=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #318# 0 100) 
COMMENT                                   (LOGPEN 0 12 13395507) 10 1 2.61251 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #367=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "additional AAV sequences" "@N" 1 -14.3295 
COMMENT                                   -8.71165 0 -10 10.9064 1.11561 #366#) 
COMMENT                             #368=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #318# 0 100) 
COMMENT                                   (LOGPEN 0 12 13395507) 10 1 6.1017 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #369=(CLabel (CWidget 0 (0 0) 1 2 0 0 #310# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "MCS 5' end" 
COMMENT                                   "@N" 1 8.86921 10.454 0 -10 4.74552 1.11561 
COMMENT                                   #368#) 
COMMENT                             #370=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #314# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.9179 
COMMENT                                    6.0912 1 0.082322) 0.8 1.8 0) 
COMMENT                             #371=(CLabel (CWidget 0 (0 0) 1 2 0 0 #310# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Ple253" "@N" 1 
COMMENT                                   13.0031 3.48466 0 -10 2.79736 1.11561 #370#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #372=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil -126 100) 
COMMENT                            (CObjectList 
COMMENT                             #373=(CLine 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #329# 543716457 
COMMENT                                    100) (LOGPEN 0 8 32768) 8 0.835356 2.64051 
COMMENT                                   2.83387) 
COMMENT                             #374=(CLabel (CWidget 0 (0 0) 1 2 0 0 #310# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40" "@N" 1 
COMMENT                                   -7.57988 -10.454 0 -10 2.21457 1.11561 #373#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #375=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1277 100) 
COMMENT                            (CObjectList 
COMMENT                             #376=(CScratch 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #318# 0 100) 
COMMENT                                   (LOGPEN 0 12 13395507) 10 1 6.03258 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #377=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 414 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS5931" "@N" 
COMMENT                                   1 13.2131 5.22699 0 -10 4.39585 1.11561 #376#) 
COMMENT                             #378=(CScratch 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #318# 0 100) 
COMMENT                                   (LOGPEN 0 12 13395507) 10 1 3.73504 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #379=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 2082602632 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS5913" "@N" 
COMMENT                                   1 9.70192 -12.1963 0 -10 4.39585 1.11561 
COMMENT                                   #378#)) (CObjectList)) 
COMMENT                      #380=(CGroupWidget 
COMMENT                            (CWidget 34 (7 34 0) 1 2 0 0 Nil -231 100) 
COMMENT                            (CObjectList 
COMMENT                             #381=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #325# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 6.1262 6.27319 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #382=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 1668576359 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "5' ITR" "@N" 1 
COMMENT                                   3.10873 13.9386 0 -10 2.44769 1.11561 #381#) 
COMMENT                             #383=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #325# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 2.45853 2.60552 1 0.082322) 0.8 1.8 
COMMENT                                   1) 
COMMENT                             #384=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #310# 4064424 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "3' ITR" "@N" 1 
COMMENT                                   -11.3033 -6.96932 0 -10 2.44769 1.11561 #383#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #385=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1905 100) 
COMMENT                     (CObjectList 
COMMENT                      #386=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -2010 100) 
COMMENT                            (CObjectList 
COMMENT                             #387=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #317# 0 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 3.91615 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #388=(CLabel (CWidget 0 (0 0) 1 2 0 0 #319# 1 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Asc}I (2695)" "@N (@S)" 5 11.2123 
COMMENT                                   -8.71165 0 -10 4.4791 0.932452 #387#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #389=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -440 100) 
COMMENT                            (CObjectList 
COMMENT                             #390=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #317# 0 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 6.10083 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #391=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #319# 268786344 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Avr}II (198)" "@N (@S)" 5 10.9929 
COMMENT                                   8.71165 0 -10 4.36254 0.932452 #390#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #392=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -754 100) 
COMMENT                            (CObjectList 
COMMENT                             #393=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #317# 697 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 6.09295 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #394=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #319# 268786344 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Fse}I (207)" "@N (@S)" 5 12.1832 
COMMENT                                   6.96932 0 -10 4.01287 0.932452 #393#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #395=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -650 100) 
COMMENT                            (CObjectList 
COMMENT                             #396=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #317# 0 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 6.10608 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #397=(CLabel (CWidget 0 (0 0) 1 2 0 0 #319# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Eco}RI (192)" "@N (@S)" 5 5.22804 
COMMENT                                   12.1963 0 -10 4.4791 0.932452 #396#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #398=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -341 100) 
COMMENT                            (CObjectList 
COMMENT                             #399=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #317# 1 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 2.83649 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #400=(CLabel (CWidget 0 (0 0) 1 2 0 0 #319# 40 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Asi}SI (3929)" "@N (@S)" 5 -6.96182 
COMMENT                                   -13.9386 0 -10 4.72886 0.932452 #399#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #401=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #6# 0) 1 2 0 0 Nil -1596 100) 
COMMENT                            (CObjectList 
COMMENT                             #402=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #317# 50271368 
COMMENT                                    100) (LOGPEN 0 8 10053171) 8 1 2.83649 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #403=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 1734803868 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pvu}I (3929)" "@N (@S)" 5 -6.75029 
COMMENT                                   -12.1963 0 -10 4.5457 0.932452 #402#) 
COMMENT                             #404=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #317# 697 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 2.33954 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #405=(CLabel (CWidget 0 (0 0) 1 2 0 0 #309# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pvu}I (4497)" "@N (@S)" 5 -14.2779 
COMMENT                                   -1.74233 0 -10 4.59566 0.932452 #404#) 
COMMENT                             #406=(CScratch 
COMMENT                                   (CWidget 3 (1 #6# 3) 1 2 0 0 #317# 0 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 1.42525 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #407=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 61269392 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pvu}I (5542)" "@N (@S)" 5 -14.354 0 0 
COMMENT                                   -10 4.5457 0.932452 #406#)) (CObjectList)) 
COMMENT                      #408=(CGroupWidget 
COMMENT                            (CWidget 6 (10 #5# 0) 1 2 0 0 Nil -1696 100) 
COMMENT                            (CObjectList 
COMMENT                             #409=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #317# 0 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 6.24606 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #410=(CLabel (CWidget 0 (0 0) 1 2 0 0 #309# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (32)" "@N (@S)" 5 3.46879 17.4233 
COMMENT                                   0 -10 3.76311 0.932452 #409#) 
COMMENT                             #411=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #317# 80722000 
COMMENT                                    100) (LOGPEN 0 8 10053171) 8 1 6.23644 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #412=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 328132 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (43)" "@N (@S)" 5 3.5124 15.681 0 
COMMENT                                   -10 3.76311 0.932452 #411#) 
COMMENT                             #413=(CScratch 
COMMENT                                   (CWidget 3 (1 #5# 3) 1 2 0 0 #317# 697 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 3.98877 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #414=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 4063680 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (2612)" "@N (@S)" 5 12.5142 
COMMENT                                   -6.96932 0 -10 4.64561 0.932452 #413#) 
COMMENT                             #415=(CScratch 
COMMENT                                   (CWidget 4 (1 #5# 4) 1 2 0 0 #317# 697 100) 
COMMENT                                   (LOGPEN 0 8 10053171) 8 1 3.07097 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #416=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 327734 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (3661)" "@N (@S)" 5 -4.2595 
COMMENT                                   -15.681 0 -10 4.64561 0.932452 #415#) 
COMMENT                             #417=(CScratch 
COMMENT                                   (CWidget 5 (1 #5# 5) 1 2 0 0 #317# 71965160 
COMMENT                                    100) (LOGPEN 0 8 10053171) 8 1 2.49528 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #418=(CLabel (CWidget 0 (0 0) 1 2 0 0 #309# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (4319)" "@N (@S)" 5 -13.4135 
COMMENT                                   -5.22699 0 -10 4.64561 0.932452 #417#) 
COMMENT                             #419=(CScratch 
COMMENT                                   (CWidget 6 (1 #5# 6) 1 2 0 0 #317# 72175688 
COMMENT                                    100) (LOGPEN 0 8 10053171) 8 1 2.48565 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #420=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #309# 1734828899 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (4330)" "@N (@S)" 5 -14.0465 
COMMENT                                   -3.48466 0 -10 4.76217 0.932452 #419#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #421=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -864 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #422=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1806 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #423=(AnlzFontBackColorItem 421 442 33619967) 
COMMENT         #424=(AnlzFontBackColorItem 364 387 33619967) 
COMMENT         #425=(AnlzFontBackColorItem 388 388 33619967) 
COMMENT         #426=(AnlzFontBackColorItem 275 295 33619967) 
COMMENT         #427=(AnlzFontBackColorItem 2901 2921 33619967))) (CObList) 0 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     CDS             5123..5980
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6154..6742
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4185..4230
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4193..4360)
                     /vntifkey="34"
                     /label=3'\ITR
     CDS             2886..3917
                     /vntifkey="4"
                     /label=iCre
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     polyA_signal    3932..4152
                     /vntifkey="25"
                     /label=SV40
     intron          2701..2873
                     /vntifkey="15"
                     /label=Intron\cassette
     misc_feature    209..2692
                     /vntifkey="21"
                     /label=Ple253
     primer          complement(276..296)
                     /vntifkey="27"
                     /label=oEMS5931
     primer          complement(2902..2922)
                     /vntifkey="27"
                     /label=oEMS5913
BASE COUNT     1741 a      1832 c      1940 g      1657 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac ctggccacga tgctctagtg taaagcaccc 
      241 atcagatatc agacgtgaag aacccctcag taacttccta cacctgcaca ttgggcttct 
      301 ggtcggtggg tccagctgag gatctagcct ctatcaccca aagagagtca gggaaagagg 
      361 atataccatc atagcacctc tttccacatt acctcaaagc cattttcctc ttcatttgaa 
      421 aactgagagt tggtgtcctg ggcctgtctc agattgagag gtcctggaaa gccgattgga 
      481 aaatcctcta agtgggccaa aaatttctgc tcgccaatat ctacttctac atacctccag 
      541 aaattcatca ttctaaaagc atgtgattgt gcaatagaga tggggaggag agagatgaca 
      601 attccctagt ccctcctaac cactaatcat catcttacca ggcagaacat cttcctcata 
      661 gatttcagtg tgacatttat tgagattcta cattatgcct gggaataaca gacatggtcc 
      721 ttaccctcct aaattttatt atcatagcag acaacacata agctgatccc ttcaatatgg 
      781 tgggtggtgt aatagaaggt atacactgag ggtcatgaga gcaaggggct aaactgagag 
      841 ttcagaaaag gcttcccaga gaaaataaga cttgagtgga acattggagg agtaggaatt 
      901 gacaatataa agatgggtta ctttcacagg aagggccaga ggtgtgcagg acactactag 
      961 gaaattggag atgaagacaa ggtcaggtcc tgaaggtctt gtatgccagg ctagggagct 
     1021 tgacctttat cctaagggca atgggagtca ctgaacagtc aagacatgac tgctcagatt 
     1081 tgccttaggg gcatattgtg tgactgggct taggctcttt tttttttttt tgagacagag 
     1141 tcttgctctg tcgcccagtc tggagtgcag tggcgcgatc tcagctcact gcaagctctg 
     1201 cctcctgggt tcacgccatt cttcttcctc agcctcccca gtagctgaga ctacaggcac 
     1261 ccgccaccaa gcccggctaa ttttttgtat tttttagtag agatggggtt tcaccgtgtt 
     1321 agccagggtg gtctcaatct cctgaccttg tgatccgccc gcctcggcct cccaaagtgc 
     1381 tgggattaca ggcatgagcc acagtgtctg gcctgggctt aggctcttcg gagcacccaa 
     1441 gcaacatagc ctaactggga gaagagaagg agcctgtttc ctggaaattg aggcctaagt 
     1501 cagagagggc cgaagtcaca gtctgccagg atgttccttg gtcatctgtc ctgtcccttg 
     1561 caaattccta accagcaggc accaaggcca caccttgccc acaggactta acctggtacc 
     1621 accagcgaga aaccagccct gctgaaggag ggagagcaca gcctaagcaa gacaagggta 
     1681 cagactgaaa ggcaatgcct ttggagtgcc cagcccaagc cctgagatcc agcaggaacc 
     1741 tcctcttaat tgttttgcgc ggggagggag atgatagtta ctcgtccaag ttcactcagt 
     1801 gatggtgcag ttcgtggcag cgttggaata ctactttggt ttgtatcatt ccaaaccctg 
     1861 ctgtctccac gaagtcacct tccttggaga agaaggggga aatgaagaag aggaaagata 
     1921 tgaggaaaga ggagcagaga gaaagagaaa tgagtctttt aaagtggcag aggaaggaga 
     1981 atggtaaaca gcaggagcga agcggctgag gagaaagaag aggaaagaaa ggcgagacgt 
     2041 gggaggattg gaacagagac aaaagggagg agagacggac agcgacaagt ggagaaaatc 
     2101 ggcgaaactt gagtggcaga gaagtctgag cgctgagacc cggcggcccc gtgcgccttc 
     2161 ccacctggcg ccgatccact ttcctcgggg tagcggccca acccacttcg ctgccagccg 
     2221 atccctttta cccgtggcta ccgggaccac tctactctcg cccacttggc tctgcctaag 
     2281 cgtcctagcc ggagcgcggt ctctgccacg tggggagggg cgcggccgag ttgctgaaga 
     2341 gcgcttctga ttggccagag ggcggggttc ttggcgtctc gccggccaga cccctccctc 
     2401 aaaggcgggg cctggagatc cacagctgga aagggcggag ccccagcagg acagctggaa 
     2461 aggggcgggg cctgacgcgc gcggctcgcc gcggcgggct gggggcgccc tggtctgcca 
     2521 taaagtgaat gggcgccggc tgggggtggc agtacgcggt gaggctcact ccctccgaga 
     2581 gtccaggagc gcccgagcgg agaggcggcc cgggagcagg ggggcggccc ccactccggc 
     2641 cgggtgcccg gcccctggcc cctgcctgcc ctctagatcg ccgccgcagc cgggcgcgcc 
     2701 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag 
     2761 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta 
     2821 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg 
     2881 ccaccatgtc caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca 
     2941 cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg 
     3001 aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga 
     3061 acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc 
     3121 aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc 
     3181 acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga 
     3241 gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac 
     3301 gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca 
     3361 ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca 
     3421 gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca 
     3481 ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca 
     3541 agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt 
     3601 tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc 
     3661 gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact 
     3721 ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca 
     3781 tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga 
     3841 acatagtgat gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc 
     3901 tcgaggatgg ggactgagcg gccgcgatcg cagacatgat aagatacatt gatgagtttg 
     3961 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 
     4021 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 
     4081 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct 
     4141 acaaatgtgg taggtcgaaa ttcccgataa ggatcttcct agagcatggc tacgtagata 
     4201 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc 
     4261 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 
     4321 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac 
     4381 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     4441 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     4501 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     4561 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     4621 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     4681 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     4741 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     4801 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     4861 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     4921 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     4981 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     5041 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     5101 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     5161 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     5221 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     5281 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     5341 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     5401 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     5461 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     5521 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     5581 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     5641 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     5701 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     5761 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     5821 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     5881 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     5941 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     6001 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     6061 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     6121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     6181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     6241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     6301 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     6361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     6421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     6481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     6541 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     6601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     6661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     6721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     6781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     6841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     6901 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     6961 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     7021 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     7081 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     7141 tgaccatgat tacgccagat ttaattaagg 
//
