LOCUS       ybeJ253.L1LV.L2NP.pRSET       4432 bp    DNA     circular     24-JAN-2012
FEATURES             Location/Qualifiers
     misc_feature    20..39
                     /note="T7 promoter"
     misc_feature    1991..2446
                     /note="f1 origin"
     misc_feature    112..132
                     /note="His6Gly"
     misc_feature    133..162
                     /note="T7 gene10 leader"
     misc_feature    166..192
                     /note="Xpress epitope"
     CDS             202..954
                     /note="ybeJ1-253"
                     /translation="GSAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDY
                     SNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFS
                     DTIFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAK
                     DHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWEIVGKPQSQEAYGCMLRKDDP
                     QFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP"
     misc_feature    955..1230
                     /note="GFP148-238"
     misc_feature    1231..1248
                     /note="Linker"
     misc_feature    1249..1689
                     /note="cpGFP1-147"
     misc_feature    1690..1770
                     /note="ybeJ254-280"
ORIGIN      
          1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT
         61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT
        121 CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC
        181 GATGACGATA AGGATCGATG GGGATCCgcc gcaggcagca cgctggacaa aatcgccaaa
        241 aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtgc ctttctctta ttacgacaat
        301 cagcaaaaag tggtgggtta ctcgcaggat tactccaacg ccattgttga agcagtgaaa
        361 aagaaactca acaaaccgga cttgcaggta aaactgattc cgattacctc acaaaaccgt
        421 attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac caacaacgtc
        481 gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac gcgcctgttg
        541 accaaaaagg gtggcgatat caaagatttt gccaacctga aagacaaagc cgtagtcgtc
        601 acttccggca ctacctctga agttttgctc aacaaactga atgaagagca aaaaatgaat
        661 atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga aagcggtcgt
        721 gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa agcgaagaaa
        781 ccagacaact gggaaatcgt cggcaagccg cagtctcagg aggcctacgg ttgtatgttg
        841 cgtaaagatg atccgcagtt caaaaagctg atggatgaca ccatcgctca ggtgcagacc
        901 tccggtgaag cggaaaaatg gtttgataag tggttcaaaa atccaattct ggtgAGCCAC
        961 AACGTCTATA TCATGGCCGA CAAGCAGAgG AACGGCATCA AGGCGAACTT CAAGATCCGC
       1021 CACAACATCG AGGACGGCGG CGTGCAGCTC GCCTACCACT ACCAGCAGAA CACCCCCATC
       1081 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CAAACTTTCG
       1141 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG
       1201 ATCACTCTCG GCATGGACGA GCTGTACAAG GGCGGTACCG GAGGGAGCAT GGTGAGCAAG
       1261 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
       1321 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
       1381 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
       1441 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
       1501 TTCAAGTCCG CCATGCCCGA AGGCTACATC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
       1561 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
       1621 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
       1681 AACTTTAACa accccctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc
       1741 aaagaaccga atgacaaggc actgaaataa GAATTCGAAG CTTGATCCGG CTGCTAACAA
       1801 AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT
       1861 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATC
       1921 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
       1981 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
       2041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
       2101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
       2161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
       2221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
       2281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
       2341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
       2401 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC
       2461 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
       2521 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
       2581 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
       2641 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
       2701 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
       2761 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA
       2821 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
       2881 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
       2941 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
       3001 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
       3061 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
       3121 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
       3181 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
       3241 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
       3301 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
       3361 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
       3421 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
       3481 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA
       3541 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
       3601 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
       3661 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
       3721 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC
       3781 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
       3841 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
       3901 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
       3961 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
       4021 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
       4081 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
       4141 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
       4201 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
       4261 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
       4321 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
       4381 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AG
//
