LOCUS       pAC-1-QFBDAD-G4MD                  9328 bp ds-DNA     circular SYN 16-Jul-2004
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      Olena Riabinina, Potter Lab, Johns Hopkins University School of
            Medicine
FEATURES             Location/Qualifiers
     CDS             201..1058
                     /note=Amp
     promoter        2240..4793
                     /note=actin5c_promoter
     misc_feature    4799..4802
                     /note=Kozak
     gene            join(4803..>5351,<7182..7685)
                     /note=QFrco
     gene            <5352..>7181
                     /note=GAL4
     polyA_site      7692..8906
                     /note=actin5c_polyA
BASE COUNT      2619 A      2237 C      2034 G      2438 T         0 OTHER
ORIGIN      ?
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
      121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
      181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
      241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
      301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
      361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
      421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
      481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
      541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
      601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
      661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
      721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
      781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
      841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
      901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
      961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
     1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
     1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
     2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
     2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
     2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
     2221 ACCATGATTA CGAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAAATCATG
     2281 AATGGCATCA ACTCTGAATC AAATCTTTGC AGATGCACCT ACTTCTCATT TCCACTGTCA
     2341 CATCATTTTT CCAGATCTCG CTGCCTGTTA TGTGGCCCAC AAACCAAGAC ACGTTTTATG
     2401 GCCATTAAAG CTGGCTGATC GTCGCCAAAC ACCAAATACA TAATGAATAT GTACACATTC
     2461 GAGAAAGAAG CGATCAAAGA AGCGTCTTCG GGCGGAGTAG GAGAATGCGG AGGAGAAGGA
     2521 GAACGAGCTG ATCTAGTATC TCTCCACAAT CCAATGCCAA CTGACCAACT GGCCATATTC
     2581 GGAGCAATTT GAAGCCAATT TCCATCGCCT GGCGATCGCT CCATTCTTGG CTATATGTTT
     2641 TTCACCGTTA CCCGGGGCCA TTTTCAAAGA CTCGTCGGCA AGATAAGATT GTGTCACTCG
     2701 CTGTCTCTCT TCATTTGTCG AAGAATGCTG AGGAATTTCG CGATGACGTC GGCGAGTATT
     2761 TTGAAGAATG AGAATAATTT GTATTTATAC GAAAATCAGT TAGTGGAATT TTCTACAAAA
     2821 ACATGTTATC TATAGATAAT TTTGTTGCAA AATATGTTGA CTATGACAAA GATTGTATGT
     2881 ATATACCTTT AATGTATTCT CATTTTCTTA TGTATTTATA ATGGCAATGA TGATACTGAT
     2941 GATATTTTAA GATGATGCCA GACCAAAAGG CTTGAATTTC TGCGTCTTTT GCCGAACGCA
     3001 GTGCATGTGC AATTGTTGTT TTTTGGAATA TTCAATTTTC GGACTGTCCG CTTTGATTTC
     3061 AGTTTCTTGG CTTATTCAAA AAGCAAAGTA AAGCCAAAAA AGCGAGATGG CAATACCAAA
     3121 TGCGGCAAAA CGGTAGTGGA AGGAAAGGGG TGCGGGGCAG CGGAAGGAAG GGTGGGGCGG
     3181 GGCGTGGCGG GGTCTGTGGC TGGGCGCGAC GTCACCGACG TTGGAGCCAC TCCTTTGACC
     3241 ATGTGTGCGT GTGTGTATTA TTCGTGTCTC GCCACTCGCC GGTTGTTTTT TTCTTTTTAT
     3301 GCTGCGCTCT CTCTAGCGCC ATCTCGCTTA CGCATGCTCA ACGCACCGCA TGTTGCCGTT
     3361 TCCTTTTATG CGTCATTTTG GCTCGAAATA GGCAATTATT TAAACAAAGA TTAGTCAACG
     3421 AAAACGCTAA AATAAATAAG TCTACAATAT GGTTACTTAT TGCCATGTGT GTGCAGCCAA
     3481 CGATAGCAAC AAAAGCAACA ACACAGGTGG CTTTCCCTCT TTCACTTTTT GTTTGCAAGC
     3541 CGCGTGCGAG CAAGACGGCA CGACCGGCAA ACGCAATTAC GCTGACAAAG AGCAGACGAA
     3601 GTTTTGGCGA AAAACATCAA GGCGCCTGAT ACGAATGCAT TTGCAATAAC AATTGCGATA
     3661 TTTAATATTG TTTATGAAGC TGTTTGACTT CAAAACACAC AAAAAAAAAA ATAAAACAAA
     3721 TTATTTGAAA GAGAATTAGG AATCGGACGC TTATCGTTAG GGTAACAACA AGAAATGCTT
     3781 ACTGAGTCAC AGCCTCTGGA AAACTGCCGC AAGCCAGAGA GAGAGAGAAA AAGAGGGAGA
     3841 GCAGCTTAGA CCGCATGTGC TTGTGTGTGA GGCGTCTCTC TCTTCGTCTC TGTTGCGCAA
     3901 ACGCATAGAC TGCACTGAAA AAATCGATTA CCTATTTTTT ATGAATGAAT ATTTGCACTA
     3961 TTACTATTCA AAACTATTAA GATAGCAATC ACATTCAATA GCCAAATACT ATACCACCTG
     4021 AGCGATGCAA CGAAATGATC AATTTGAGCA AAAATGCTGC ATATTTAGGA CGGCATCATT
     4081 ATAGAAATGC TTCTTGCTGT GTACTTTTCT CTCGTCTGGC AGCTGTTTCG CCGTTATTGT
     4141 TAAAACCGGC TTAAGTTAGG TGTGTTTTCT ACGACTAGCT AGTGAATGCC CTACTAGAAG
     4201 ATGTGTGTTG CACAAAATGT CCCTGGAATA ACCAATTTGA AGTGCAGATA GCAGTAAACG
     4261 TAAGCTAATA TGAATATTAT TTAACTGTAA TGTTTTAATA TCGCTGGACA TTACTAATAA
     4321 ACCCACTATA AACACATGTA CATATGTATG TTTTGGCATA CAATGAGTAG TTGGGGAAAA
     4381 AATGTGTAAA AGCACCGTGA CCATCACAGC ATAAAGATAA CCAGCTGAAG TATCGAATAT
     4441 GAGTAACCCC CAAATTGAAT CACATGCCGC AACTGATAGG ACCCATGGAA GTACACTCTT
     4501 CATGGCGATA TACAAGACAC ACACAAGCAC GAACACCCAG TTGCGGAGGA AATTCTCCGT
     4561 AAATGAAAAC CCAATCGGCG AACAATTCAT ACCCATATAT GGTAAAAGTT TTGAACGCGA
     4621 CTTGAGAGCG GAGAGCATTG CGGCTGATAA GGTTTTAGCG CTAAGCGGGC TTTATAAAAC
     4681 GGGCTGCGGG ACCAGTTTTC ATATCACTAC CGTTTGAGTT CTTGTGCTGT GTGGATACTC
     4741 CTCCCGACAC AAAGCCGCTC CATCAGCCAG CAGTCGTCTA ATCCAGAGAC CCCGGATCCA
     4801 ACATGCCACC CAAGCGCAAA ACGCTTAACG CTGCGGCTGA GGCTAACGCT CATGCCGACG
     4861 GACACGCCGA CGGAAACGCC GACGGACACG TGGCCAATAC GGCCGCGTCC TCGAATAATG
     4921 CGAGGTTCGC TGATCTCACT AACATCGATA CTCCGGGTCT GGGACCCACA ACTACGACCC
     4981 TGCTCGTGGA ACCAGCACGC TCAAAGCGTC AACGAGTGTC CCGCGCATGC GACCAGTGCC
     5041 GTGCAGCCCG AGAGAAATGC GACGGAATAC AGCCTGCGTG TTTCCCGTGC GTTTCCCAGG
     5101 GAAGGTCCTG CACTTATCAG GCTTCGCCGA AAAAGAGGGG AGTTCAAACC GGTTATATTC
     5161 GTACGCTGGA GCTCGCCCTC GCCTGGATGT TTGAAAATGT CGCGCGTTCC GAAGATGCCT
     5221 TGCATAACCT CCTCGTCCGT GACGCCGGAC AAGGATCAGC TCTGCTCGTT GGTAAAGATT
     5281 CGCCGGCTGC CGAGCGACTC CATGCCCGTT GGGCTACTAG CCGTGTCAAT AAGAGCATTA
     5341 CCCGCCTCCT CATTGACTCG GCAGCTCATC ATGATAACTC CACAATTCCG TTGGATTTTA
     5401 TGCCCAGGGA TGCTCTTCAT GGATTTGATT GGTCTGAAGA GGATGACATG TCGGATGGCT
     5461 TGCCCTTCCT GAAAACGGAC CCCAACAATA ATGGGTTCTT TGGCGACGGT TCTCTCTTAT
     5521 GTATTCTTCG ATCTATTGGC TTTAAACCGG AAAATTACAC GAACTCTAAC GTTAACAGGC
     5581 TCCCGACCAT GATTACGGAT AGATACACGT TGGCTTCTAG ATCCACAACA TCCCGTTTAC
     5641 TTCAAAGTTA TCTCAATAAT TTTCACCCCT ACTGCCCTAT CGTGCACTCA CCGACGCTAA
     5701 TGATGTTGTA TAATAACCAG ATTGAAATCG CGTCGAAGGA TCAATGGCAA ATCCTTTTTA
     5761 ACTGCATATT AGCCATTGGA GCCTGGTGTA TAGAGGGGGA ATCTACTGAT ATAGATGTTT
     5821 TTTACTATCA AAATGCTAAA TCTCATTTGA CGAGCAAGGT CTTCGAGTCA GGTTCCATAA
     5881 TTTTGGTGAC AGCCCTACAT CTTCTGTCGC GATATACACA GTGGAGGCAG AAAACAAATA
     5941 CTAGCTATAA TTTTCACAGC TTTTCCATAA GAATGGCCAT ATCATTGGGC TTGAATAGGG
     6001 ACCTCCCCTC GTCCTTCAGT GATAGCAGCA TTCTGGAACA AAGACGCCGA ATTTGGTGGT
     6061 CTGTCTACTC TTGGGAGATC CAATTGTCCC TGCTTTATGG TCGATCCATC CAGCTTTCTC
     6121 AGAATACAAT CTCCTTCCCT TCTTCTGTCG ACGATGTGCA GCGTACCACA ACAGGTCCCA
     6181 CCATATATCA TGGCATCATT GAAACAGCAA GGCTCTTACA AGTTTTCACA AAAATCTATG
     6241 AACTAGACAA AACAGTAACT GCAGAAAAAA GTCCTATATG TGCAAAAAAA TGCTTGATGA
     6301 TTTGTAATGA GATTGAGGAG GTTTCGAGAC AGGCACCAAA GTTTTTACAA ATGGATATTT
     6361 CCACCACCGC TCTAACCAAT TTGTTGAAGG AACACCCTTG GCTATCCTTT ACAAGATTCG
     6421 AACTGAAGTG GAAACAGTTG TCTCTTATCA TTTATGTATT AAGAGATTTT TTCACTAATT
     6481 TTACCCAGAA AAAGTCACAA CTAGAACAGG ATCAAAATGA TCATCAAAGT TATGAAGTTA
     6541 AACGATGCTC CATCATGTTA AGCGATGCAG CACAAAGAAC TGTTATGTCT GTAAGTAGCT
     6601 ATATGGACAA TCATAATGTC ACCCCATATT TTGCCTGGAA TTGTTCTTAT TACTTGTTCA
     6661 ATGCAGTCCT AGTACCCATA AAGACTCTAC TCTCAAACTC AAAATCGAAT GCTGAGAATA
     6721 ACGAGACCGC ACAATTATTA CAACAAATTA ACACTGTTCT GATGCTATTA AAAAAACTGG
     6781 CCACTTTTAA AATCCAGACT TGTGAAAAAT ACATTCAAGT ACTGGAAGAG GTATGTGCGC
     6841 CGTTTCTGTT ATCACAGTGT GCAATCCCAT TACCGCATAT CAGTTATAAC AATAGTAATG
     6901 GTAGCGCCAT TAAAAATATT GTCGGTTCTG CAACTATCGC CCAATACCCT ACTCTTCCGG
     6961 AGGAAAATGT CAACAATATC AGTGTTAAAT ATGTTTCTCC TGGCTCAGTA GGGCCTTCAC
     7021 CTGTGCCATT GAAATCAGGA GCAAGTTTCA GTGATCTAGT CAAGCTGTTA TCTAACCGTC
     7081 CACCCTCTCG TAACTCTCCA GTGACAATAC CAAGAAGCAC ACCTTCGCAT CGCTCAGTCA
     7141 CGCCTTTTCT AGGGCAACAG CAACAGCTGC AATCATTAGT GCGTCAGTTG GAGCTCCCTC
     7201 CTACCGCCAC GGCTACGGCC TCGATAATGC CGCACGTGAT GGAGCAGCCT CTCAGTACCA
     7261 GCATTAACCC CGTCAACGAC CGCTTCAACG GTATTCCCAA CCCCACTCCG TATAACTCCG
     7321 ATGCAGCTCT CGATGCTATC ACTCAGACCA ACGATTATGG AAGCGTAAAT ACACATGGTA
     7381 TCCTCTCTAC TTACCCGCCA CCGGCTACGC ACCTTAATGA AGCTTCCGTC GCTCTCGCTC
     7441 CCGGTGGCGC CCCCCCCCGA CCGCCTCCTC CGTATGTTGA CAGCACGACC AATCACCCGC
     7501 CGTACCACTC GAATCTGGTT CCAATGGCGA ACTTTGGTTA CTCGACCGTT GATTACGATG
     7561 CCATGGTTGA CGATTTGGCT AGCATTGAAT ACACGGACGC TGTGGATGTC GACCCACAGT
     7621 TTATGACCAA TCTGGGATTC GTTCCTGGAT GTAACTTCTC CGACATTAAT ACATACGAAC
     7681 AGTGAGGCCG CGTTAACCGA TCCGTCGACC ATGAAGATCA AGATCATTGC CCCGCCAGAG
     7741 CGCAAGTACT CTGTCTGGAT CGGTGGCTCC ATCCTGGCTT CGCTGTCCAC CTTCCAGCAG
     7801 ATGTGGATCT CCAAGCAGGA GTACGACGAG TCCGGCCCCT CCATTGTGCA CCGCAAGTGC
     7861 TTCTAAGAAG GATCGCTTGT CTGGGCAAGA GGATCAGGAT CGGGATGGTC TTGATTCTGC
     7921 TGGAGGAGGA GGAGGAGAAG TCGAGGAAGC AGCAGCGAAA GTGCAAGTGC GAGTGGTGGA
     7981 AGTTTGGAGT GCAGCACAAC AAAATCAACA ACAACACCAA CTACAAGATG AAAAGAGCGG
     8041 AACCACCTGC ACACCATCAT CACTATCATC ATCGTTTTGG GCGCATGTTG TGTGGTTCCA
     8101 GCGTATTAAT ATAATTAATT TATTCCACAT GAGATATGAT ATGATATACT ATGTATTTTT
     8161 TGTTTTTTTT TTATTTGTAA ACCTTTAATA TAACAAGAAC TACAAAAAAT GAAAATGAGC
     8221 GAAAATGCAT ATTCTGCCAT TCCACACACA CACCAACAAC ACCCAACACA CGCACACCCA
     8281 CAAGCTTACA CACACACATT CGCGGCATGA CAAGGACATC AAGATAAAGA AGAACTTAAA
     8341 GAAGATATTT CCCAAAGCGC AAAAAGAACA CACACACATT GCAAAACACA AACAACACAC
     8401 TAGCGTTTTG TACAATTCGT CAGCAACCTT ATGTATTATT TTTTAATTAT GATGTAATTA
     8461 TAAACAAAGT GAAACAAAAA TATGAAAACA AAAAGGAAAA TCAAATCTGT CTTCTCTTTC
     8521 TCCCGCTCTC CTCGCTCTCT GCTGCTAACC TCGCCCTCTC CTCTCTCATC TTTTTGTCTG
     8581 TCTCTCTTCC ACATTTTTGC CGGCCGGCAA AATAATAACC CACACACACT CACACTTGGC
     8641 TGCAGTTTCG CGTGCGATAT TCACACACAT TCAAGCATAC AAGCATACAT ACATATGTAT
     8701 TTTTTTTTTA TTTGTACACT TTTCTAATTG CATGCGTATC GATTGATAAG TTTACGCCTG
     8761 AAAATGTTAA TTAAAATGTG AAAATGCAAC TGAAAAACTG ATGAAATGAA ACAACAACAA
     8821 GCGAACAATT TGCTACATGT GTATTGTCTA ACAACCGTTA CTGCAACGGT TGCTTCGGAA
     8881 AAGGGTGAAG AGGAAGAGGG CACGAGGTCG ACCTGCAGGC ATGCAAGCTT GGCACTGGCC
     8941 GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA
     9001 GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC
     9061 CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT
     9121 CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA
     9181 TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG
     9241 CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG
     9301 TTTTCACCGT CATCACCGAA ACGCGCGA
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