LOCUS       pAAV-EF1a-hChR2-        7010 bp    DNA     circular     23-AUG-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|452192721|
COMMENT     VNTDBDATE|455453634|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-EF1a-hChR2-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|Feng Zhang|
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COMMENT                          "Foreign object. Author: Feng Zhang. Original author: Feng Zhang" 
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     misc_feature    139..144
                     /note="MluI"
     misc_feature    145..150
                     /note="BsrGI"
     misc_feature    1112..1171
                     /note="0.5 (NI) - all 4 available"
     misc_feature    1172..1360
                     /note="TAL C-term 63aa"
     misc_feature    1098..1103
                     /note="BsiWI"
     misc_feature    4845..4850
                     /note="BsaI"
     misc_feature    1092..1097
                     /note="BsaI rec"
     misc_feature    1104..1109
                     /note="BsaI rec"
     exon            151..635
                     /dnas_title="Human Synapsin I promoter"
                     /vntifkey="61"
                     /label=Human Synapsin I promoter
     misc_feature    636..641
                     /note="BamHI"
     misc_feature    651..677
                     /note="HA tag core seq"
     misc_feature    642..647
                     /note="kozak"
     misc_feature    678..1085
                     /note="TALE N-terminal 136 aa"
     misc_feature    2813..3044
                     /note="bGH polyA"
     misc_feature    2832..3022
                     /note="oligo"
     misc_feature    2997..3006
                     /note="G-ACAGCA-G"
     misc_feature    1087..1090
                     /note="BsaI overhang"
     misc_feature    1111..1114
                     /note="BsaI Overhang"
     misc_feature    1367..1399
                     /note="NLS (SPKKKRKVEAS)"
     misc_feature    1400..1774
                     /note="SID4x"
     misc_feature    1781..1843
                     /note="2A"
     misc_feature    1844..2173
                     /note="phiLOV2.1"
     misc_feature    1361..1366
                     /note="NheI"
     misc_feature    1775..1780
                     /note="NcoI"
     misc_feature    1145..1150
                     /note="RVD"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
         61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
        121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
        181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
        241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
        301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
        361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
        421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
        481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
        541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
        601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cGCCACCATG TACCCATACG
        661 ATGTTCCAGA TTACGCTGTA GATTTGAGAA CTTTGGGATA TTCACAGCAG CAGCAGGAAA
        721 AGATCAAGCC CAAAGTGAGG TCGACAGTCG CGCAGCATCA CGAAGCGCTG GTGGGTCATG
        781 GGTTTACACA TGCCCACATC GTAGCCTTGT CGCAGCACCC TGCAGCCCTT GGCACGGTCG
        841 CCGTCAAGTA CCAGGACATG ATTGCGGCGT TGCCGGAAGC CACACATGAG GCGATCGTCG
        901 GTGTGGGGAA ACAGTGGAGC GGAGCCCGAG CGCTTGAGGC CCTGTTGACG GTCGCGGGAG
        961 AGCTGAGAGG GCCTCCCCTT CAGCTGGACA CGGGCCAGTT GCTGAAGATC GCGAAGCGGG
       1021 GAGGAGTCAC GGCGGTCGAG GCGGTGCACG CGTGGCGCAA TGCGCTCACG GGAGCACCCC
       1081 TCAACCTGAC Agagacccgt acgggtctcG ACTCACGCCT GAGCAGGTAG TGGCTATTGC
       1141 ATCCAAtATC GGGGGCAGAC CCGCACTGGA GTCAATCGTG GCCCAGCTTT CGAGGCCGGA
       1201 CCCCGCGCTG GCCGCACTCA CTAATGATCA TCTTGTAGCG CTGGCCTGCC TCGGCGGACG
       1261 ACCCGCCTTG GATGCGGTGA AGAAGGGGCT CCCGCACGCG CCTGCATTGA TTAAGCGGAC
       1321 CAACAGAAGG ATTCCCGAGA GGACATCACA TCGAGTGGCA gCTAGCAGCC CCAAGAAgAA
       1381 GAGaAAGGTG GAGGCCAGCA TGAACATCCA GATGCTGCTG GAGGCCGCTG ACTACCTGGA
       1441 ACGGAGAGAG CGCGAAGCCG AGCACGGATA TGCTTCAATG CTGCCCGGAA GCGGCATGAA
       1501 TATTCAGATG CTGCTGGAGG CTGCTGATTA CCTGGAAAGG CGCGAACGGG AGGCCGAACA
       1561 TGGCTATGCT TCCATGCTGC CTGGGTCTGG AATGAATATC CAAATGCTGC TGGAGGCAGC
       1621 CGATTACCTG GAACGGAGAG AAAGAGAAGC CGAGCACGGA TACGCCAGCA TGCTGCCAGG
       1681 CAGCGGGATG AACATACAAA TGCTGCTGGA GGCTGCCGAT TACCTGGAGA GGCGCGAGAG
       1741 AGAAGCTGAA CATGGCTATG CCTCTATGCT GCCCccatgg GGCAGTGGAG AGGGCAGAGG
       1801 AAGTCTGCTA ACATGCGGTG ACGTCGAGGA GAATCCTGGC CCAATCGAGA AGTCCTTCGT
       1861 AATAACAGAT CCAAGACTGC CTGATTACCC CATCATTTTT GCCAGCGACG GTTTTCTGGA
       1921 GCTCACCGAA TATTCCAGGG AGGAAATCAT GGGGAGAAAC GCCAGGTTCC TGCAAGGGCC
       1981 TGAGACAGAC CAGGCCACCG TTCAGAAGAT TCGCGATGCT ATCCGCGATC AGAGGGAAAC
       2041 CACTGTGCAG TTAATAAACT ACACTAAGTC AGGCAAAAAG TTCTGGAATC TGCTTCATCT
       2101 CCAACCTGTC CGGGATCGAA AGGGAGGGCT GCAATACTTC ATCGGTGTGC AGCTGGTGGG
       2161 AAGTGATCAT GTGTAAgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
       2221 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
       2281 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
       2341 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
       2401 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
       2461 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
       2521 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
       2581 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctAtgttg ccacctggat
       2641 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
       2701 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
       2761 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agCTAGAGCT
       2821 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
       2881 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
       2941 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
       3001 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGAggtaac cacgtgcgga
       3061 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
       3121 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
       3181 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
       3241 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
       3301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
       3361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
       3421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
       3481 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
       3541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
       3601 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
       3661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
       3721 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
       3781 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
       3841 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
       3901 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
       3961 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
       4021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
       4081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
       4141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
       4201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
       4261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
       4321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
       4381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
       4441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
       4501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
       4561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
       4621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
       4681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
       4741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
       4801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
       4861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
       4921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
       4981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
       5041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
       5101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
       5161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       5221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
       5281 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
       5341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
       5401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
       5461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
       5521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
       5581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
       5641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
       5701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       5761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//

