LOCUS       pOSIP-KL_(KanR,_        6588 bp ds-DNA     circular     25-JUL-2013
DEFINITION  .
ACCESSION   urn.local...1370032569110.25
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  . .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..1170
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    35..450
                     /modified_by="User"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     CDS             142..447
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     rep_origin      451..1124
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      1170..1232
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     misc_feature    complement(1213..1235)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="137011706291352600000cb2e1e-272d-4168-b
                     aa1-72238684ee7d"
                     /%GC="52.2"
                     /Tm="63.5"
                     /Hairpin_Tm="37.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAACGCAAAAAGGCCATCCGTC"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /note="Geneious type: primer_bind_reverse"
     terminator      complement(1247..1339)
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     misc_recomb     1384..5024
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_recomb     1384..1417
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     CDS             complement(1423..2136)
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     protein_bind    complement(2147..2163)
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     protein_bind    complement(2170..2186)
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     protein_bind    complement(2194..2210)
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     CDS             2378..3448
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_feature    3579..4561
                     /created_by="User"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     CDS             3675..4490
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      4501..4541
                     /created_by="User"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     rep_origin      4581..5010
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     primer_bind     4986..5003
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_recomb     5011..5024
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_recomb     5025..5058
                     /ApEinfo_label="FRT site sequence"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_recomb     5071..5457
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      complement(5464..5735)
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     primer_bind     complement(5524..5541)
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      6040..6208
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     terminator      6223..6391
                     /ApEinfo_label="rrnBT1 terminator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     terminator      6406..6574
                     /ApEinfo_label="rrnBT1 terminator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#c0c0c0"
     primer_bind     6565..6587
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="13701144647861780000b303456-6608-49ea-8
                     4ec-8e5d8b07794d"
                     /%GC="47.8"
                     /Tm="60.6"
                     /Hairpin_Tm="47.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAAACTGCCAGGAATTGGGGATC"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
ORIGIN      
        1 gaattcgagc tcggtacccg gggatcctct agagggctta ctaaaagcca gataacagta
       61 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
      121 tatgtcaaaa agaggtatgc tatgcagttt aaggtttaca cctataaaag agagagccgt
      181 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      241 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      301 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      361 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      421 attaacctga tgttctgggg aatataataa cccgtagaaa agatcaaaag atcttcttga
      481 gatccttttt ttctgcgcgt aatctgctac ttgcaaacaa aaaaaccacc gctaccagcg
      541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggctttagc
      601 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
      661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1081 gcctttttac ggttcctggc cttttgctgg ccttttgctc acattactag tagcggccgc
     1141 tgcaggcatg cctcgagatg catggcgcct aacctaaact gacaggcatc aaattaagca
     1201 gaaggccatc ctgacggatg gcctttttgc gtttcgaaca attgaaaaaa cctcgcgcct
     1261 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttactga
     1321 atttcggtgg tgccgttaat taaccggtgg gccctcatga taataatggt ttcttagacg
     1381 tccgaagttc ctattctcta gaaagtatag gaacttccct agtcagccaa acgtctcttc
     1441 aggccactga ctagcgataa ctttccccac aacggaacaa ctctcattgc atgggatcat
     1501 tgggtactgt gggtttagtg gttgtaaaaa cacctgaccg ctatccctga tcagtttctt
     1561 gaaggtaaac tcatcacccc caagtctggc tatgcagaaa tcacctggct caacagcctg
     1621 ctcagggtca acgagaatta acattccgtc aggaaagctt ggcttggagc ctgttggtgc
     1681 ggtcatggaa ttaccttcaa cctcaagcca gaatgcagaa tcactggctt ttttggttgt
     1741 gcttacccat ctctccgcat cacctttggt aaaggttcta agcttaggtg agaacatccc
     1801 tgcctgaaca tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact
     1861 aaccgcttca tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct
     1921 aactttgaga atttttgtaa gcaatgcggc gttataagca tttaatgcat tgatgccatt
     1981 aaataaagca ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga
     2041 taagccaagt tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag
     2101 ctgctcttgt gttaatggtt tcttttttgt gctcatacgt taaatctatc accgcaaggg
     2161 ataaatatct aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcat
     2221 gtactaagga ggttgtatac gcgtcgtcga tgggttcggg aatgcaggat attcccacct
     2281 ccggttaagg atggaagaga gtatctgttc cacgaatcag cggtaaaggt tgacttaaat
     2341 cgaccagtaa caggtggcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga
     2401 gcgccgggat ttacccccta acctttatat aagaaacaat ggatattact gctacaggga
     2461 cccaaggacg ggtaaagagt ttggattagg cagagacagg cgaatcgcaa tcactgaagc
     2521 tatacaggcc aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat
     2581 caacagtgat aattccgtta cgttacattc atggcttgat cgctacgaaa aaatcctggc
     2641 cagcagagga atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag
     2701 gaggggtctg cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct
     2761 caatggatac atagacgagg gcaaggcggc gtcagccaag ttaatcagat caacactgag
     2821 cgatgcattc cgagaggcaa tagctgaagg ccatataaca acaaaccatg tcgctgccac
     2881 tcgcgcagca aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat
     2941 ttatcaagca gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt
     3001 taccgggcaa cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata
     3061 tctttatgtc gagcaaagca aaacaggcgt aaaaattgcc atcccaacag cattgcatat
     3121 tgatgctctc ggaatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg
     3181 agaaaccata attgcatcta ctcgtcgcga accgctttca tccggcacag tatcaaggta
     3241 ttttatgcgc gcacgaaaag catcaggtct ttccttcgaa ggggatccgc ctacctttca
     3301 cgagttgcgc agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca
     3361 acatcttctc gggcataagt cggacaccat ggcatcacag tatcgtgatg acagaggcag
     3421 ggagtgggac aaaattgaaa tcaaataatg attttatttt gactgatagt gacctgttcg
     3481 ttgcaacaaa ttgataagca atgccaagct tggcactggc tgatcagcta gcccatgggt
     3541 atggacagtt ttccctttga tatgtaacgc acgttgtgtc tcaaaatctc tgatgttaca
     3601 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     3661 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcccgt ccgcgcttaa
     3721 actccaacat ggacgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     3781 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     3841 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtccgtctc aactggctga
     3901 cggagtttat gcctctcccg accatcaagc attttatccg tactcctgat gatgcgtggt
     3961 tactcaccac cgcgattcct gggaaaacag ccttccaggt attagaagaa tatcctgatt
     4021 caggtgaaaa tattgttgat gcgctggccg tgttcctgcg ccggttacat tcgattcctg
     4081 tttgtaattg tccttttaac agcgatcgtg tatttcgtct tgctcaggcg caatcacgca
     4141 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     4201 aacaagtctg gaaagaaatg cacaagctct tgccattctc accggattca gtcgtcactc
     4261 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     4321 atgttggacg ggtcggaatc gcagaccgtt accaggacct tgccattctt tggaactgcc
     4381 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     4441 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa taatactagc
     4501 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gattaccgcc tttgagtgag
     4561 cgtcgaccta gtgcggccgc aagatccggc cacgatgcgt ccggcgtaga ggatctgaag
     4621 atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc
     4681 tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact
     4741 aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga
     4801 acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa gttttataag
     4861 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
     4921 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
     4981 ggaacactta acggctgaca tgggaattag gaagttccta ttccgaagtt cctattctct
     5041 agaaagtata ggaacttcca tatgccatgg catcacagta tcgtgatgac agaggcaggg
     5101 agtgggacaa aattgaaatc aaataatgat tttattttga ctgatagtga cctgttcgtt
     5161 gcaacaaatt gataagcaat gcttttttat aatgccaact tagtataaaa aagctgaacg
     5221 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     5281 tacataatac tgtaaaacac aacatatatg cagtcactat gaatcaacta cttagatggt
     5341 attagtgacc tgtaacagag cattagcgca aggtgatttt tgtcttcttg cgctaatttt
     5401 ttgtcatcaa acctgtcgca ctccagagaa gcacaaagcc tcgcaatcca gtgcaaagct
     5461 agcttcttcg tctgtttcta ctggtattgg cacaaacctg attccaattt gagcaaggct
     5521 atgtgccatc tcgatactcg ttcttaactc aacagaagat gctttgtgca tacagcccct
     5581 cgtttattat ttatctcctc agccagccgc tgtgctttca gtggatttcg gataacagaa
     5641 aggccgggaa atacccagcc tcgctttgta acggagtaga gacgaaagtg attgcgccta
     5701 cccggatatt atcgtgagga tgcgtcatcg ccattaattc actgatcagt gatagctgtc
     5761 aaacatgaga attgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     5821 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5881 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     5941 cgtagcgata gcggagtgta tgctgcacat gacattaacc tataaaaata ggcgtatcac
     6001 gaggcccttt cgtcttcaag aattaattcc caattcccca ggcatcaaat aaaacgaaag
     6061 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     6121 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     6181 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
     6241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     6301 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     6361 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
     6421 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     6481 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     6541 gggtggcggg caggacgccc gccataaact gccaggaatt ggggatcg
//
