LOCUS 48668\M-SPcas 9563 bp DNA circular SYN 25-SEP-2013 DEFINITION pcDNA3.3 TOPO TA. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|674084946| COMMENT VNTDBDATE|674084946| COMMENT LSOWNER| COMMENT VNTNAME|48668 M-SPcas| COMMENT VNTAUTHORNAME|Kevin Esvelt| COMMENT VNTAUTHOREML|kesvelt@gmail.com| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList)) COMMENT #278=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -695 100) COMMENT (CObjectList COMMENT #279=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 82 100) COMMENT (CObjectList COMMENT #280=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #185# 907 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.18809 1.9 COMMENT 0.082322 1) COMMENT #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #187# 42 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Asc}I (4704)" "@N (@S)" 5 0 0 -2.56623 COMMENT 3.45752 2.94872 0.615385 #280#)) COMMENT (CObjectList)) COMMENT #282=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 8 100) COMMENT (CObjectList COMMENT #283=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #185# 1031 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.62048 1.9 COMMENT 0.082322 1) COMMENT #284=(CLabel (CWidget 0 (0 0) 1 2 0 0 #187# 82 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Sbf}I (996)" "@N (@S)" 5 0 0 -12.8941 COMMENT 12.3243 2.61538 0.615385 #283#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #285=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 73 100) COMMENT (CObjectList) (CObjectList)) COMMENT #286=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 2082602612 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2082602632 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_feature 130..417 /vntifkey="21" /label=CAG_enhancer promoter 51..627 /vntifkey="30" /label=CMV_immearly_promoter misc_feature 584..604 /vntifkey="21" /label=CMV_fwd_primer promoter 585..654 /vntifkey="30" /label=CMV_promoter misc_feature 628..647 /vntifkey="21" /label=pCEP_fwd_primer misc_feature 630..654 /vntifkey="21" /label=LNCX_primer promoter 597..716 /vntifkey="30" /label=CMV2_promoter terminator 4936..5206 /vntifkey="43" /label=TK_PA_terminator rep_origin 5260..5566 /vntifkey="33" /label=f1_origin misc_feature complement(5680..5700) /vntifkey="21" /label=pBABE_3_primer misc_feature complement(5686..5901) /vntifkey="21" /label=SV40_enhancer promoter 5698..5966 /vntifkey="30" /label=SV40_promoter rep_origin 5865..5942 /vntifkey="33" /label=SV40_origin misc_feature 5927..5946 /vntifkey="21" /label=SV40pro_F_primer CDS 6081..6875 /gene="NeoR/KanR" /vntifkey="4" /label=NeoR/KanR /note="ORF frame 2" gene 6084..6872 /gene="NeoR/KanR" /vntifkey="60" terminator 7054..7173 /vntifkey="43" /label=SV40_PA_terminator misc_feature 7142..7161 /vntifkey="21" /label=EBV_rev_primer misc_feature complement(7217..7235) /vntifkey="21" /label=M13_reverse_primer misc_feature complement(7234..7256) /vntifkey="21" /label=M13_pUC_rev_primer promoter complement(7270..7299) /vntifkey="30" /label=lac_promoter rep_origin complement(7608..8227) /vntifkey="33" /label=pBR322_origin CDS complement(8382..9242) /gene="Ampicillin" /vntifkey="4" /label=Ampicillin /note="ORF frame 2" gene complement(8382..9242) /gene="Ampicillin" /vntifkey="60" promoter complement(9284..9312) /vntifkey="30" /label=AmpR_promoter misc_feature 4852..4884 /vntifkey="21" /label=NLS CDS 748..4851 /vntifkey="4" /label=SPcas BASE COUNT 2549 a 2400 c 2393 g 2221 t ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgatatc 781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa 841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc 901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga 961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct 1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa 1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag 1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg 1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag 1261 ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact 1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc 1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg 1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac 1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac 1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg 1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag 1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac 1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc 1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa 1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta 1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc 1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc 2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat 2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag 2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc 2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac 2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca 2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc 2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag 2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg 2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag 2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg 2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc 2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga 2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg 2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca 2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca 2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg 3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag 3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg 3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc 3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat 3241 cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct 3301 attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc 3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg 3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg 3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg 3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga 3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag 3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca 3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga 3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag 3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca 3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa 3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag 4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc 4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac 4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa 4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga 4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa 4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa 4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa 4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat 4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag 4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt 4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac 4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata 4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca 4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct 4861 gaccccaaga agaagaggaa ggtgtgactc tagatgaaag ggttcgatcc ctaccggtta 4921 gtaatgagtt taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 4981 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt 5041 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 5101 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 5161 ggcccagggc tcgcagccaa cgtcggggcg gcagaccctg ccatagcaga tctgcgcagc 5221 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 5281 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 5341 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc 5401 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 5461 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 5521 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 5581 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat 5641 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 5701 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 5761 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 5821 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 5881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 5941 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 6001 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 6061 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 6121 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 6181 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 6241 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 6301 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 6361 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 6421 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 6481 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 6541 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 6601 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 6661 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 6721 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 6781 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 6841 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg 6901 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 6961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 7021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 7081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 7801 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 8041 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 9361 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca 9421 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 9481 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 9541 attgcatgaa gaatctgctt agg //