LOCUS pCAG-Golden-Gate-Esp3I-Destination 5233 bp DNA circular UNA 5-JUL-2011 ACCESSION pCAG-cre-bpA COMMENT This file is created by Vector NTI http://www.informaxinc.com/ FEATURES Location/Qualifiers Promoter 800..2467 /label="CAG Promoter" Promoter 2477..2499 /label=T7 Restriction_sit join(2510^2511,2516..2521) /label="Esp3I (CACC)" Misc._feature complement(2522..2797) /vntifkey="21" /label=lacZ' /label=lacZ' Promoter complement(2798..2919) /label="lacZ promoter" Restriction_sit join(2920..2925,2926^2927) /label="Esp3I (GTCA)" PolyA_signal 2934..3204 /label=bpA CDS 3992..4852 /label=Amp ORIGIN 1 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 61 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 121 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 181 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 241 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 301 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 361 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 421 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 481 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 541 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 601 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 661 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 721 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 781 CATGAGGCGC GCCGGATTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG 841 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC 901 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC 961 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT 1021 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT 1081 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT 1141 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TCGAGGTGAG CCCCACGTTC 1201 TGCTTCACTC TCCCCATCTC CCCCCCCTCC CCACCCCCAA TTTTGTATTT ATTTATTTTT 1261 TAATTATTTT GTGCAGCGAT GGGGGCGGGG GGGGGGGGGG GGCGCGCGCC AGGCGGGGCG 1321 GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC CAATCAGAGC 1381 GGCGCGCTCC GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC CTATAAAAAG 1441 CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG TTGCCTTCGC CCCGTGCCCC GCTCCGCGCC 1501 GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT GAGCGGGCGG 1561 GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT CGTTTCTTTT 1621 CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG GGGGGAGCGG 1681 CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC CGCGCTGCCC 1741 GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT GTGCGCGAGG 1801 GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA ACAAAGGCTG 1861 CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG TCGGGCTGTA 1921 ACCCCCCCCT GCACCCCCCT CCCCGAGTTG CTGAGCACGG CCCGGCTTCG GGTGCGGGGC 1981 TCCGTGCGGG GCGTGGCGCG GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG CAGGTGGGGG 2041 TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG CGCGGCGGCC 2101 CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC 2161 GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGG CGGAGCCGAA ATCTGGGAGG 2221 CGCCGCCGCA CCCCCTCTAG CGGGCGCGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA 2281 TGGGCGGGGA GGGCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCAT CTCCAGCCTC 2341 GGGGCTGCCG CAGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT 2401 CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT 2461 CCTACAGATC CTTAATTAAT AATACGACTC ACTATAGGGG GTCGACCCGC CACCGGAGAC 2521 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC 2581 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG 2641 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA GCGCGCGTAA TACGACTCAC 2701 TATAGGGCGA ATTGGGTACC GGGCCCCCCC TCGAGGTCCT CCAGCTTTTG TTCCCTTTAG 2761 TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 2821 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 2881 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCC GTCTCCgtca GAGCTCGCTG 2941 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 3001 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 3061 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 3121 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 3181 TGAGGCGGAA AGAACCAGCT GGGGCTCGAG ATCCACTAGT TCTAGCCTCG AGGCTAGAGC 3241 GGCCGCCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 3301 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 3361 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAGCG 3421 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 3481 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 3541 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 3601 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 3661 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 3721 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 3781 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA 3841 AAATATTAAC GCTTACAATT TAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 3901 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 3961 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 4021 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 4081 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 4141 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 4201 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 4261 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 4321 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 4381 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 4441 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 4501 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 4561 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 4621 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 4681 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 4741 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 4801 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 4861 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 4921 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 4981 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 5041 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 5101 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 5161 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 5221 GCCTACATAC CTC //