LOCUS met25p-MCP-mcher 6561 bp DNA circular SYN 30-MAY-2013 DEFINITION met25p-MCP-mcherry rotated to position 4140 ACCESSION met25p-MCP-mcher KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 6561) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: Fragment 2 Fragment ends: XhoI and BamHI Fragment size: 5808 Insert molecule: ch Fragment ends: BamHI and XhoI Fragment size: 745 FEATURES Location/Qualifiers misc_feature 33..414 /gene="met25p" /SECDrawAs="Region" CDS 421..1518 /gene="MCP-mCherry" /SECDrawAs="Gene" misc_feature 421..786 /gene="MCP" /SECDrawAs="Info only" misc_feature 796..1518 /gene="cherry" /SECDrawAs="Info only" misc_feature 1547..1786 /gene="cyc" /SECDrawAs="Region" CDS 2584..3384 /gene="URA3" /SECDrawAs="Gene" CDS 4515..5375 /gene="ampR" /SECDrawAs="Gene" ORIGIN 1 accctcacta aagggaacaa aagctggagc tccggatgca agggttcgaa tcccttagct 61 ctcattattt tttgcttttt ctcttgaggt cacatgatcg caaaatggca aatggcacgt 121 gaagctgtcg atattgggga actgtggtgg ttggcaaatg actaattaag ttagtcaagg 181 cgccatcctc atgaaaactg tgtaacataa taaccgaagt gtcgaaaagg tggcaccttg 241 tccaattgaa cacgctcgat gaaaaaaata agatatatat aaggttaagt aaagcgtctg 301 ttagaaagga agtttttcct ttttcttgct ctcttgtctt ttcatctact atttccttcg 361 tgtaatacag ggtcgtcaga tacatagata caattctatt acccccatcc atactctaga 421 atgctagccg ttaaaatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga 481 actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga atggatcagc 541 tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag 601 aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat 661 atggaactaa ccattccaat tttcgccacg aactccgact gcgagcttat tgttaaggca 721 atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc aaactccggc 781 atctacgcgg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc 841 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 901 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 961 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 1021 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 1081 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 1141 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 1201 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 1261 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 1321 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 1381 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 1441 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 1501 gacgagctgt acaagtaaat cgataccgtc gacctcgagt catgtaatta gttatgtcac 1561 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac 1621 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta 1681 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga 1741 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa 1801 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 1861 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 1921 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 1981 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 2041 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 2101 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 2161 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 2221 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 2281 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 2341 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 2401 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg 2461 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa 2521 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt 2581 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc 2641 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc 2701 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt 2761 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc 2821 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac 2881 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat 2941 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat 3001 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac 3061 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa 3121 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga 3181 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc 3241 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg 3301 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg 3361 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg 3421 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata 3481 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc 3541 aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa 3601 ttgaaaagct gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag 3661 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 3721 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 3781 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 3841 tgtcatgata ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg 3901 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt 3961 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa 4021 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga 4081 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac 4141 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg 4201 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac 4261 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat 4321 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg 4381 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 4441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 4501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 4561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 4621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 4681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 4741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 4801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 4861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 4981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 5041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 5101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 5161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 5221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 5281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 5341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 5401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 5461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 6001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 6061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 6121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 6181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 6241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 6301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 6361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 6421 ttaatgtgag ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc 6481 ctatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 6541 attacgccaa gcgcgcaatt a //