LOCUS       pEMS2117        5633 bp    DNA     circular      6-MAY-2013
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|661356789|
COMMENT     VNTDBDATE|661356789|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2117|
COMMENT     VNTAUTHORNAME|Jack Hickmott|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 60 0 2766 0 4374 0 0 
COMMENT            "") #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1158 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 198 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 192 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 207 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 1339 0 2383 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 32 0 43 0 2124 0 2782 0 
COMMENT            2793 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 2392 0 0 "") 
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COMMENT        #12=(CRSite (CStringList) "taa" "taa" 1 0 78 137 0 144 0 223 0 229 0 283 
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COMMENT             5298 0 5434 0 5493 0 5578 0 5625 0 5629 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "tta" "tta" 1 0 92 136 0 143 0 160 0 219 0 222 
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COMMENT        #14=(CRSite (CStringList) "tga" "tga" 1 0 120 21 0 77 0 140 0 310 0 335 
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COMMENT             "") 
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COMMENT             0 5365 0 5430 0 5507 0 5511 0 5586 0 0 "") 
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COMMENT             (CStringList) 1 1 1 1 "4648..5505") 
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COMMENT        #25=(CFSignal (CObList) "SV40" 25 0 0 2395 2615 0 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "Intron cassette" 15 0 0 1164 1336 0 
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COMMENT        #28=(CFSignal (CObList) "CMV i.e. enhancer" 9 0 0 209 574 0 
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COMMENT        #30=(CFSignal (CObList) "Exon1" 61 0 0 859 952 0 (CStringList) 
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COMMENT        #33=(CFSignal (CObList) "oEMS5951" 27 0 1 389 413 0 (CStringList) 
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COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     CDS             3586..4443
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4617..5205
                     /vntifkey="21"
                     /label=Origin
     misc_feature    2648..2693
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(2656..2823)
                     /vntifkey="34"
                     /label=3'\ITR
     CDS             1349..2380
                     /vntifkey="4"
                     /label=iCre
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     polyA_signal    2395..2615
                     /vntifkey="25"
                     /label=SV40
     intron          1164..1336
                     /vntifkey="15"
                     /label=Intron\cassette
     primer          complement(1365..1385)
                     /vntifkey="27"
                     /label=oEMS5913
     enhancer        209..574
                     /vntifkey="9"
                     /label=CMV\i.e.\enhancer
     intron          952..1153
                     /vntifkey="15"
                     /label=Chimeric\intron
     exon            859..952
                     /vntifkey="61"
                     /label=Exon1
     promoter        576..858
                     /vntifkey="29"
                     /label=Chicken\b-actin\promoter
     misc_feature    209..1153
                     /vntifkey="21"
                     /label=smCBA
     primer          complement(389..413)
                     /vntifkey="27"
                     /label=oEMS5951
     primer          complement(364..386)
                     /vntifkey="27"
                     /label=oEMS5997
BASE COUNT     1299 a      1471 c      1500 g      1363 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccgg taccctagtt attaatagta atcaattacg 
      241 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 
      301 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 
      361 atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt acggtaaact 
      421 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 
      481 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 
      541 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc 
      601 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt 
      661 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg 
      721 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc 
      781 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 
      841 cgaagcgcgc ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc 
      901 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
      961 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
     1021 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ctagagcctc tgctaaccat 
     1081 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 
     1141 catcattttg gcaaaggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa 
     1201 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 
     1261 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 
     1321 aggtgtccac tcccaggcgg ccgccaccat gtccaacctg ctgactgtgc accaaaacct 
     1381 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt 
     1441 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc 
     1501 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt 
     1561 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca 
     1621 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa 
     1681 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc 
     1741 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa 
     1801 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct 
     1861 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg 
     1921 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa 
     1981 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc 
     2041 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc 
     2101 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct 
     2161 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc 
     2221 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca 
     2281 ggctggtggc tggaccaatg tgaacatagt gatgaactac atcagaaacc tggactctga 
     2341 gactggggcc atggtgaggc tgctcgagga tggggactga gcggccgcga tcgcagacat 
     2401 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 
     2461 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 
     2521 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt 
     2581 tttttaaagc aagtaaaacc tctacaaatg tggtaggtcg aaattcccga taaggatctt 
     2641 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc 
     2701 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 
     2761 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 
     2821 cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     2881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
     2941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
     3001 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     3061 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     3121 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     3181 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     3241 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     3301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     3361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     3421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     3481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     3541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     3601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     3661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     3721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     3781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     3841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     3901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     3961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     4021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     4081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     4141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     4201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     4261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     4321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     4381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     4441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     4501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     4561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     4621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     4681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     4741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     4801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     4861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     4921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     4981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     5041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     5101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     5161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     5221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     5281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     5341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     5401 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     5461 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     5521 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     5581 caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta agg 
//
