LOCUS pSicoRMS2-Luc 8181 bp DNA circular 21-OCT-2007 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|460809157| COMMENT VNTDBDATE|460809157| COMMENT LSOWNER| COMMENT VNTNAME|pSicoRMS2-Luc| COMMENT VNTAUTHORNAME|Matthew Spindler| COMMENT VNTAUTHORTEL|415-734-2833| COMMENT VNTAUTHORFAX|415-355-0960| COMMENT VNTAUTHOREML|mspindler@gladstone.ucsf.edu| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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-6.96932 0 -10 3.87134 0.799245 #257#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #259=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -177 100) COMMENT (CObjectList) (CObjectList)) COMMENT #260=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -735 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 39101488 COMMENT (CStringList) 51118231 4064024 (CObList))) FEATURES Location/Qualifiers misc_feature 1076..1213 /vntifkey="21" /label=Psi misc_feature 1707..1910 /vntifkey="21" /label=RRE promoter 2631..2943 /vntifkey="29" /label=U6\Promoter misc_feature 2825..2858 /vntifkey="21" /label=loxP misc_feature 4981..5014 /vntifkey="21" /label=loxP misc_feature 5070..5449 /vntifkey="21" /label=WRE primer 2481..2500 /vntifkey="27" /label=Lox\Replace\For\Primer primer complement(493..512) /vntifkey="27" /label=Lox\Replace\Rev\Primer misc_marker 3594..4301 /vntifkey="22" /label=mCherry promoter 3029..3571 /vntifkey="29" /label=EF-1alpha\promoter misc_feature 4365..4958 /vntifkey="21" /label=Puro misc_feature 4308..4361 /vntifkey="21" /label=T2A misc_feature complement(7191..8048) /vntifkey="21" /label=AmpR misc_feature 2944..2998 /vntifkey="21" /label=Luc\shRNA BASE COUNT 2031 a 2107 c 2163 g 1880 t ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2821 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 2881 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 2941 gtttcttacg ctgagtactt cgattcaaga gatcgaagta ctcagcgtaa gttttttctc 3001 gagtactagg atccattagg cggccgcgga tctgcgatcg ctccggtgcc cgtcagtggg 3061 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg 3121 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc 3181 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt 3241 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt 3301 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc 3361 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt 3421 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca 3481 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc 3541 gttacagatc caagctgtga ccggcgccta cgctagcgct accggtcgcc accatggtga 3601 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 3661 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 3721 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 3781 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 3841 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 3901 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 3961 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 4021 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 4081 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 4141 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 4201 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 4261 agggccgcca ctccaccggc ggcatggacg agctgtacaa gccccgggag ggcagaggaa 4321 gtcttctaac atgcggtgac gtggaggaga atcccggccc tcgaaccgag tacaagccca 4381 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt 4441 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 4501 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 4561 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 4621 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 4681 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 4741 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 4801 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 4861 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag 4921 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta agaattcgtc gagggaccta 4981 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact 5041 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 5101 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5161 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5221 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5281 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5341 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5401 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5461 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5521 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5581 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5641 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga 5701 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 5761 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 5821 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 5881 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 5941 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg 6001 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac 6061 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta 6121 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac 6181 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6361 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 6721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 6781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 6841 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 6901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 6961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 7141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 7201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 7261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 7321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 7381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 7441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 7501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 7561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 7621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 7681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 7741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 7801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 7861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 7921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 7981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 8041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 8101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 8161 ccccgaaaag tgccacctga c //