LOCUS       pSV40\7xmutmiR-1        5409 bp    DNA     circular SYN 24-FEB-2012
DEFINITION  Cloning vector pGL3-Control, complete sequence.
ACCESSION   U47296
VERSION     U47296.2  GI:13195704
SOURCE      Cloning vector pGL3-Control.
  ORGANISM  Cloning vector pGL3-Control
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Groskreutz,D.J. and Schenborn,E.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1996) D.J. Groskreutz, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Kenefick,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Technical Writing, Promega Corporation,
            2800 Woods Hollow Road, Madison, WI 53711-5399, USA
  REMARK    Sequence update by submitter
COMMENT     On Mar 5, 2001 this sequence version replaced gi:1200462.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618693383|
COMMENT     VNTDBDATE|618693383|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSV40 7xmutmiR-122T|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pGL3-Control\">Cloning vector pGL3-Control(Cloning vector pGL3-Control)</a>" 
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FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Cloning vector pGL3-Control"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45858"
                     /vntifkey="98"
                     /note="luciferase reporter vector"
     misc_feature    1..41
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="multiple cloning site"
     promoter        48..250
                     /vntifkey="30"
                     /label=Promoter_P_1
     gene            280..1932
                     /gene="luc+"
                     /vntifkey="60"
     CDS             280..1932
                     /gene="luc+"
                     /codon_start=1
                     /product="luciferase"
                     /protein_id="AAA89084.1"
                     /db_xref="GI:1200463"
                     /vntifkey="4"
                     /label=luc+
     primer_bind     complement(281..303)
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="GL primer2 sequencing primer binding site"
     polyA_signal    2117..2338
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="SV40 late poly(A) signal"
     enhancer        2358..2594
                     /vntifkey="9"
                     /label=Enhancer_1
     primer_bind     complement(2652..2671)
                     /vntifkey="28"
                     /label=Primer_Bind_2
                     /note="RV primer4 sequencing primer binding site"
     rep_origin      2909..2909
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1-derived plasmid replication origin"
     gene            complement(3671..4531)
                     /gene="AmpR"
                     /vntifkey="60"
     CDS             complement(3671..4531)
                     /gene="AmpR"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="AAA89085.1"
                     /db_xref="GI:1200464"
                     /vntifkey="4"
                     /label=AmpR
                     /note="ampicillin resistance"
     rep_origin      4663..5118
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="f1 origin"
     polyA_signal    5249..5402
                     /vntifkey="25"
                     /label=PolyA_Signal_2
                     /note="upstream poly(A) signal"
     primer_bind     5351..5370
                     /vntifkey="28"
                     /label=Primer_Bind_3
                     /note="RV primer3 sequencing primer binding site"
     misc_feature    1940..2093
                     /vntifkey="21"
                     /label=mutmiR122\sponge
BASE COUNT     1428 a      1313 c      1291 g      1377 t 
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta 
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc 
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
      241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata 
      301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat 
      361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc 
      421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg 
      481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa 
      541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac 
      601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc 
      661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa 
      721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc 
      781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat 
      841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt 
      901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt 
      961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt 
     1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga 
     1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg 
     1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct 
     1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt 
     1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca 
     1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca 
     1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga 
     1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg 
     1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac 
     1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat 
     1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca 
     1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg 
     1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca 
     1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc 
     1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag 
     1921 atcgccgtgt aattctagac aaacaccata caacaagaaa caaacaccat acaacaagaa 
     1981 acaaacacca tacaacaaga aacaaacacc atacaacaag aaacaaacac catacaacaa 
     2041 gaaacaaaca ccatacaaca agaaacaaac accatacaac aagaaagggc ccgtcggggc 
     2101 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 
     2161 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 
     2221 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 
     2281 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa 
     2341 atcgataagg atctgaacga tggagcggag aatgggcgga actgggcgga gttaggggcg 
     2401 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca 
     2461 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat 
     2521 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac 
     2581 acacattcca cagcggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 
     2641 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 
     2701 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc 
     2761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     2821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     2881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     2941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     3001 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     3061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     3121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     3181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     3241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     3301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     3361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     3421 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     3481 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     3541 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     3601 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     3661 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     3721 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     3781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     3841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     3901 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     3961 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     4021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     4081 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     4141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     4201 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     4261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     4321 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     4381 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     4441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     4501 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     4561 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     4621 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg 
     4681 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     4741 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     4801 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     4861 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     4921 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     4981 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
     5041 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 
     5101 tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 
     5161 cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta 
     5221 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg 
     5281 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa 
     5341 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct 
     5401 ctatcgata 
//
