LOCUS       pEMS1488               20459 bp    DNA     circular     16-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364 - GCGTATCACGAGGCCCTTTC Tm=56.0 Deg C
            Antisense primer: oEMS2668 - GCCAAGTAGGAAAGTCCCATAAGG Tm=56.3 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            349 bp product
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|546104413|
COMMENT     VNTDBDATE|546104413|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1488|
COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
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COMMENT        (mapper: 23.2135 -16.2652 40.0378 40.0378 0.01 10 -1 20459 20459 1 0 0) 
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COMMENT                            -10 6.2441 1.47361 Nil) 
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COMMENT               #143=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1162 100) 
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COMMENT                                   "FFRT (F5 mutant)" "@N" 1 13.6816 6.96932 0 
COMMENT                                   -10 7.99245 1.17389 #147#) 
COMMENT                             #149=(CScratch 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 16.8439 
COMMENT                                   -1.74233 0 -10 11.0645 1.17389 #151#) 
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COMMENT                                   "Kozak consensus" "@N" 1 14.5547 5.22699 0 
COMMENT                                   -10 7.99245 1.17389 #153#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -4.33355 12.1963 0 -10 2.87229 1.17389 #162#) 
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COMMENT                      #166=(CGroupWidget 
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COMMENT                                   0.082322 1) 
COMMENT                             #168=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   1 3.34777 13.9386 0 -10 4.79547 1.17389 #167#) 
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COMMENT                                   "5' homology sequence" "@N" 1 -16.2027 
COMMENT                                   3.48466 0 -10 10.4901 1.17389 #172#) 
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COMMENT                                   "3' homology sequence" "@N" 1 -8.39455 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #174#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 16.5913 
COMMENT                                   -6.96932 0 -10 14.9609 1.17389 #176#)) 
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COMMENT               #178=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -185 100) 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #180=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -255 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     misc_recomb     14322..18133
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(19232..20453)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     12871..14296
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     9230..12875
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     5276..9229
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    14303..14320
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1902..1949
                     /vntifkey="21"
                     /label=FFRT\(F5\mutant)
     misc_feature    5020..5066
                     /vntifkey="21"
                     /label=FFRT
     misc_feature    5220..5270
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    1950..1960
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1769..1901
                     /vntifkey="21"
                     /label=Intron
     misc_feature    1961..5019
                     /vntifkey="21"
                     /label=lacZ
     misc_feature    128..1754
                     /vntifkey="21"
                     /label=CAG
                     /note="1313"
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     primer          379..402
                     /vntifkey="27"
                     /label=oEMS2668
BASE COUNT     5122 a      4776 c      4978 g      5583 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctctgc aggcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      421 tcatcgctat taacatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 
      481 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 
      541 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 
      601 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 
      661 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga 
      721 gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 
      961 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 
     1021 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 
     1081 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 
     1141 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 
     1201 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 
     1261 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 
     1321 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg 
     1381 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     1441 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     1501 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     1621 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     1681 cggcggctct agacaattgt actaaccttc ttctctttcc tctcctgaca ggttggtgta 
     1741 cagtagcttc caccacgcgt ggcgcgccgt aagtatcaag gttacaagac aggtttaagg 
     1801 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc 
     1861 tattggtctt actgacatcc actttgcctt tctctccaca ggaagttcct attccgaagt 
     1921 tcctattctt caaaaggtat aggaacttcc gccaccatgg cggatcccgt cgttttacaa 
     1981 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 
     2041 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 
     2101 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc 
     2161 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg 
     2221 cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg 
     2281 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc 
     2341 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg 
     2401 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac 
     2461 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt 
     2521 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc 
     2581 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat 
     2641 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac 
     2701 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg 
     2761 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt 
     2821 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg 
     2881 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc 
     2941 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga 
     3001 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg 
     3061 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat 
     3121 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa 
     3181 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg 
     3241 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt 
     3301 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat 
     3361 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac 
     3421 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc 
     3481 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg 
     3541 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac 
     3601 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat 
     3661 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt 
     3721 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg 
     3781 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc 
     3841 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg 
     3901 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc 
     3961 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg 
     4021 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg 
     4081 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc 
     4141 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt 
     4201 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat 
     4261 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac 
     4321 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg 
     4381 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat 
     4441 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc 
     4501 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg 
     4561 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca 
     4621 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc 
     4681 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg 
     4741 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc 
     4801 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat 
     4861 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg 
     4921 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt 
     4981 cgctaccatt accagttggt ctggtgtcaa aaataataaa agttcctatt ccgaagttcc 
     5041 tattctctag aaagtatagg aacttcagcg gccgcgactc tagatcataa tcagccatac 
     5101 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 
     5161 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 
     5221 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc gaattctcgt 
     5281 aaaactcttc atggcagtag ttattattct ctctctctct ctttttcttt tttcttgaga 
     5341 caggatattt ctctgttgcc caggctggag tgcagtggca cagtcttggc tcactgcagc 
     5401 ctggacctcc tgggctcaag ccatcctccc acctcagcct cccaagtagc tggggctaca 
     5461 ggcacatggc caccaggccc agataatttt tcatttttgt agagactgag tctcaccatg 
     5521 ttacccaggt ttattattct cattttttag atgaagagac tgaggtccag agaagctcaa 
     5581 tgacttgcct agttttacaa atctcctgcc atcacatacc cctcagcgtc cttaataaga 
     5641 gggaggccac caactatgtg ctgggcactg tggtggatgc tggagctata gggttgagta 
     5701 tataagaaat ggtgttgctg gagcaactgt tgcttgctta cctgacctat ctgagaatta 
     5761 attagcaggg gaacatattt ttgttttcag attcaatata agaacttgtg tgggcaaaaa 
     5821 taaagatcag tagtaataac agtagttccc atttgctgac tgtactgtcc taagtgcata 
     5881 tatatataca tacacacacg catacctata ctcctctaat actcaaaatg atcctgttta 
     5941 tgtattgtta atatgctcat tttattttta aatttttatt tatttttatt tttatttatt 
     6001 tttgagacgg agtctcattc tgtcgcggag gctgaagtgc agtggtgcga tctcagctca 
     6061 gtgcgacctc cgcctcccgg gttcaagtga ttctcctgcc tcagcctccg gactagctgg 
     6121 gattacaggc gcccgcctcc acgcccagct aatttttgta tttttagtag agatggggtt 
     6181 tcgccatgtt ggccaggctg gtctcgtact cctgaccttg agtgatccac ctgcctcggc 
     6241 ctcccaaagt gctgggatta caggcatgag ccaccgcgcc gggctaatat gctcatttta 
     6301 gtgaggcaaa aatagaggct cagagtctga tttgtacaaa actacagagc agttaagtgt 
     6361 cctctcagat gtgtaccctg atctgggtga ctctaggact ctaggtctca actgttacaa 
     6421 ccagttaagg gtttggggaa gcactgggcc aagagtcagg aaaatggaag ccacaggtag 
     6481 tgcaaggtct tgggaatggg acgtctggtc caaggattca cgcgatgact ggaacccgaa 
     6541 gagccggggc ccggtttacg gccgccatga agcaacgcgc gccggtaggt ttgggaatca 
     6601 gggagccctc tgaataggag actgagttgg gagggaaagg ggcttcgctg ggggagcctc 
     6661 ggcttcttct gggagaaaat tcccacggct acctagtgag cctgcaaact ggtaggcgcc 
     6721 ggcgtaggcg cgcgggcggg gccgggggcg gggcctgcgg ggcgtggcgg ggcgggcaga 
     6781 gggcggggcc tgcttctcct cagcttcagg cggctgcgac gagccctcag gcgaacctct 
     6841 cggctttccc gcgcggcgcc gcctcttgct gcgcctccgc ctcctcctct gctccgccac 
     6901 cggcttcctc ctcctgagca gtcagcccgc gcgccggccg gctccgttat ggcgacccgc 
     6961 agccctggcg tcgtggtgag cagctcggcc tgccggccct ggccggttca ggcccacgcg 
     7021 gcaggtggcg gccgggccct gaggcgcggg atcgatccat gacacccccc cgccccccat 
     7081 atctcactat aattcactgt caaatggctt ttatgatttg gagtttgggg atacttttaa 
     7141 agacagggtc ttggtagctc ctagatggct tgggattcac ttagtaaccc ttgctggcct 
     7201 aaaattcagg aacttctacc tcatcttccc aagtactggg ttagtaggga tgtccagcat 
     7261 atttagcttg ctttaatgat tttgctgtac ttttttctgt gtgtacaggt gcatgtatat 
     7321 ttataaaact aacataatat attttacccc caaagtacca taaccctaat taatgctatt 
     7381 aaatgttttg tatgctgggc tctgggcata ctaatataac caatattata caatgcaaaa 
     7441 caattttgga atatcttctg taaaattatc ttattagtaa gttgtaacca tctcagactt 
     7501 ctaagtttga gtttgtaaag gatcagtttt ttttggtgac ctacacacac acacacacac 
     7561 acacacacac acacacacac aggggggtgg attctgagaa tttagagaat ttagatggtt 
     7621 aataccagtt tttattttta ttctgtgtga tatttattta ttaagattta tttatttatt 
     7681 acatgtaggt acactgtatc tgtcttcaga cacaccagaa gagggcatca gatctcgtta 
     7741 cagatggttg tgagtcacca tgtggttgct gggatttgaa ctcaggacct ctggaagaac 
     7801 agtcagtgct cttaaccgtt gagccatctc accagccccg tgatatttta tttaacttac 
     7861 tagaatatac tgtgttaaaa catattttag gggattgggg agatggccca ttctgtaaag 
     7921 tgtctgccat gtcatcttca ggacctgcgt ttgtaacccc agcatggaga agacagagac 
     7981 agggagaccc ctgggcttcc tggcaagcta gactagcaaa tttggttaac taggttcaat 
     8041 gagagactca gtctcaaaac ataagaagtg aattttttag acccaatttc aatctctgaa 
     8101 ctccatacgt atgcacatgt acctgcatat aaatatatat acacacccat aaacacatgc 
     8161 acacacaaaa aatggttcaa aagttcttaa aatgtataag agtagaaagt gtagagccgg 
     8221 gagtggtggc gcacgccttt aatcccagca cttgggagca gaggcaggtg gatttctgag 
     8281 ttcgaggcca gcctggtcta cagagtgagt tccaggacag ccagagctat acagagaaat 
     8341 cctgcctcaa aaaaccaaaa ataaagtgta tatactaatc tttcatgcct aaaatattta 
     8401 tttttgccac atttttcata cctctttaat atttttcttt ttctctttgt gcttcaaagg 
     8461 aaattttgtg tcatgtttta cttttcattc ttgactctat tcagcagtaa gacgcagcat 
     8521 ggctttctta gagaagtata atctattaca aaacggtaac atttaactgc tctacatacc 
     8581 ctcttgtagc taaattcctc tgttaaacta agacttatta tctaaacgta attaaatgtt 
     8641 gatatcttat aatgctccac tttgaaacag ctgtctttct tttgcagatt agcgatgatg 
     8701 aaccaggtta tgacctagat ttgttttgta tacctaatca ttatgccgag gatttggaaa 
     8761 aagtgtttat tcctcatgga ctgattatgg acaggttagt aagacctcga ttgaagttat 
     8821 ttttagaggg tgtgtgtgtg gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 
     8881 aagagagaga ggagaggcat gtgtgatata tatgtgaagt gtattgcatg tacatggaat 
     8941 ccagagaagg acattaggtg tcctactctg ttgctctctt attcccttga atcagtgtct 
     9001 cttgctgaac ctggggcata catactgatt tggctaggct gcctggctag caagacccag 
     9061 ggatctgctt gtctctgccc cccagtgctg acgttacaag caggtgcaac cacatctggt 
     9121 ttttatatgg gtactggggg atctgaactt agggcctgat gcgtgcccaa gtgctctcac 
     9181 ccgccaagcc acctctccag ccaaagggtt ttttttcttg tattaattct aacttagcaa 
     9241 ctgttgagta gttgatgttt gaatataagc taagccaact tattagaata aatgttttct 
     9301 attttttact tgtatgtgtg agtgcctgca tgtatgtatg tgaagcatat gtattccagg 
     9361 atgtcagaat tgtgatggat tccctggaac tggagttaca gaccattgtg aaccactgtg 
     9421 tatgggtgct gggaactgaa ctttaggtcc tctgcaaaaa cagtaaggat cttaactgat 
     9481 aaactatctt tctagcccta aggctttcta cttttaaagt tcgtttctct ttctctctct 
     9541 ctctctctct ctctctctct ctctccctct ccctccctcc tcctcctcct cctctttccc 
     9601 ccctctcgcc tcctctttct ctctcccctc cctctctctc tctcacacac acacacagac 
     9661 agagagagga gagagaaagg aagccttact aattaattac ctagttttag acataactag 
     9721 tgggaatcca tagtaagtaa atcaacattc ttgaagagag gttagaagaa ttggctctcg 
     9781 ttgtccatcg aggctgtata agattattct tctgttttct cagcttacca tacatatttt 
     9841 ctacgtagac aataccaatt caaatacact gtacgtaaag tatatgcgca cacacacaca 
     9901 cacacacaca cacacacaca cacaagttac atattgtcat atagtactaa aatttatgga 
     9961 ctttttcata ttaatacatc tagagctatc atgtttttta tcttttaaaa attactttac 
    10021 ggggctacag agatgtctca gcagttagga gcactgactg ctcttccaga ggtcctgagt 
    10081 tcaattccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatccactct 
    10141 tatggtgtgt ctgaagacag tgacagtgta ctcatataca tgaaataaat ctttaaaaaa 
    10201 ttactttacc atcagggtat atatatatat atatatgtgt gtgtgtgtgt gtgtgtgtat 
    10261 gaagtatttg ctatttgaga gggtctcact gtttagctca gtctggcttg gaacgttctg 
    10321 tatagcccag actggcctca actcagcagt ccccctgaaa ggctctcata tgtggagatt 
    10381 acaggtatgt gctactgtgc ccagccctac atgttacttt gtttttgaaa tagggtatca 
    10441 aatagcttag actggcttct aagtcagtgt gtagctaagg atggtctgag cttctgaacc 
    10501 tgctacttca actcctggtg tgccatcatg gccagtttgc acagttgtga gagtagaact 
    10561 caggacctcc tatgtgttac tagacatgtt ctctaccagc tgagctacat cccaattcgc 
    10621 ctacatttac ttttattttt taaaagattt atttatgtat acagcactct gcctgcatgt 
    10681 acatctgcat gccagaagag ggcaccagat ctcatcatac atagttgtga gccaccatgt 
    10741 ggttgctggg gattgaactc agggcctctg gaagagcagt cagtgctctt aacctctgag 
    10801 ccatctctgt agccccctac atttatttta aagattatag gctattctat tcatagatgt 
    10861 gccataactg atttaaccag atcttcatta ttttgtactt agtagagtat attagaggga 
    10921 atgaatctta aatacacatt ttgacacttg ctcagcttta gacaagtaaa aatcactggt 
    10981 caaggaaatg gtgcttagtg gatgcttctg aactgtcaaa ttgtcctcca acaagttaca 
    11041 tcattttata cttccaacgt tatgtgagag ttcatttttc tttaggctct tctagtattt 
    11101 tatatttgag aaataaaatt taaccaaaat ttgccaatct agtaagcttg tttttatttg 
    11161 tatctttaaa ttttatgata gatgttatag tgtactctcc tctcccctcc cctcccctct 
    11221 cctcctcagc aggatcttgt tatgtagccc taagggatct aatacttact ctgtagtcca 
    11281 ggctgtcctt cagctcatga gacacaccat catgcttggc atgtttcttg ttctgaaagg 
    11341 tatctggttt taattttggt tgagctggtg tgttggtgta tatctgtaat ctcagcacag 
    11401 gcaagtagag acaaggatta tgattacagt ccagcctggg ctatttagag ggattctctt 
    11461 taatctttcc tcatgcccca aaatcttacc tttggtatat gaaaaatagt ctccacttct 
    11521 gcaaaatatt gctttatgaa gtaagaatta attcccttca tagagacaag gaatgtgtcc 
    11581 tgtaaaagtt taatgtgtaa gaagtatttg ttataaaaga taaatattca gaatcttctt 
    11641 tttaattcct gattttattt ctataggact gaaagacttg ctcgagatgt catgaaggag 
    11701 atgggaggcc atcacattgt ggccctctgt gtgctcaagg ggggctataa gttctttgct 
    11761 gacctgctgg attacattaa agcactgaat agaaatagtg atagatccat tcctatgact 
    11821 gtagatttta tcagactgaa gagctactgt gtaagtataa ttaacttata attaaaaaaa 
    11881 tagggccatt ctagttttat ctatattttt tttaaacttg tgcaaactat gctacataat 
    11941 caacttactt gattatgtaa tatatatagt aatagttgga attactatta ttacatattt 
    12001 atttttctgt gctggggtag aacccaggga actaagtact gtaataaccg agcttcattc 
    12061 atcctagtca tttttaattt tttttttttt ttttgttttt tcgagacaag gtttctctgt 
    12121 gtagccctgg ctgtcctgga actcactctg tagaccaggc tggcctcgaa ctcagaaatc 
    12181 tgcctgcctc tgcctcccag tgctgagatt aatgacgtgc accaccactg cctggcattt 
    12241 ttttaaagtt ttaatttgag accctttctc actaacttgc tcacactatc ctcgaactca 
    12301 ttacgtgggc tggtctagaa atttcagtca acttgcctca gcttctcaag tagctgggat 
    12361 tacaggcagg tgctaccact tctggccatg atcattgttg ttgttgctat tattattatt 
    12421 attatttcac tgatacttca tatcacaaca cagaatagta actgtagttc atgaccaaca 
    12481 aaaaagttgt acagtttctc tgttgtctta ttttgatctg gattttgatt tgtttcatgg 
    12541 cactgggcag gctaatctca aactgcatgg ctcaaatagt ccttctgtct taatctccca 
    12601 tgttctggaa gtataggtat cactacacct agctgtattt tcatatttta aaaaaaacgc 
    12661 ctaagaattg ctattgaatg aggcggagag atggctcagc agtcacaagt acttgtagag 
    12721 gactggggtt tggttactag cacctcaaag atggcttaca attatctgta gctccaattc 
    12781 cagaggatct gtgtggtaaa gctgcatgta tatgggcatg caggtaaaac tgctaaacat 
    12841 ataaaaatag ctcagtctta agagaattgg tcgacttttt cgccctttga cgttggagtc 
    12901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
    12961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
    13021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
    13081 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
    13141 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
    13201 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
    13261 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
    13321 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
    13381 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
    13441 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
    13501 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
    13561 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
    13621 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
    13681 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
    13741 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
    13801 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
    13861 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
    13921 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
    13981 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
    14041 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
    14101 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
    14161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
    14221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
    14281 aaaccaccgc taccaggtcg agtagggata acagggtaat ggatcctcgt tgggcacctg 
    14341 cactcatgta cgcaggccca catatagatg tacacacgca tatcattcaa aatagaaatt 
    14401 atttatacaa gtagtgcgtt ggagagctgg ctcaggagtt gttaatgctt gctacccttg 
    14461 caaaggaccc aagttcaatt tccagcacct gtgttgggca tctcacaacc acatgtaaat 
    14521 ctagtttcag gggatttcac ccctcttctg aactcttcag gcacttatac ccacaaatgg 
    14581 acatctccac atagagacac atacttttaa aaaagtaatc tagggtctag agaaatggtt 
    14641 atagaccacg gactgggatc agttcccaga agccacatcc tgtggctcac aattgcctgt 
    14701 gaatcaagtt ctagatctgg tggcctcgtc tagcctgcag tcatcaacaa acacatgcac 
    14761 acatccatac acacataatt taaaagaaag cgaaagtaac ctataatgct aaacaaagca 
    14821 cattctcagt gtagactgca gtggtgatga accttgaaaa tatattaata gaagccagac 
    14881 acgtaagtca caaatggtat aactcaattt atatgaaatt cccagagagt ccaaagttaa 
    14941 aaaaaacaaa aaggcagatt attggttgtc agtagctaca gagatgggac attgaagata 
    15001 ttaataggta tagggttgag gattcatggc ctttccctct tccatgttag catgtctatt 
    15061 gttgttaacc ttattcaact catgttaggg caatcatatt gatgagactt aatgagtata 
    15121 gcttgacatt cctagaagac agagtctcac atcagactct ctgatcctct gactcttaga 
    15181 atctttcaac accctcttct gaaatgatct ctgagccttt ggttgtgact gaattatgag 
    15241 aaagaatttt atgttgaatg aattttactt cagcattttt acaaaactga agctggggat 
    15301 ggcgtttcat gcgtgcagtc cctaccattt gcctataagc ctggaggagg cagaagtaga 
    15361 attaggcttt tcagggacat cttcagctgc atcatgagct tgaggacaac ttgagctaca 
    15421 taagattccc atccccaccc ccacacacac acacacatct agtatgtacc tagtatgcag 
    15481 tcttttcata tgtttaaaat gtagaaaggt atatgtgaag tctccctttt ccccacaact 
    15541 tagtccctcc aaagtattgc atcagtttcc ttgtacatga ttttatgcat atataagata 
    15601 cactatttct ctctctctct ctctccttcc ctccttccct ccaacctctg ggtatgagtt 
    15661 gagggttatg tatgtttcaa gttgtaataa gaattgccac tcaaacattt catcccattt 
    15721 taagttaagt ttgtctgatt ttccacttta gaagggcagc catgatggct catgcctata 
    15781 atcccatcct agcacttgta agcctaatac ataagactgg aggattgtca tcagcttgag 
    15841 gctagcctgg tctacctaat gagttccagc cagccaagag tagaatataa gaccctgtct 
    15901 ccaaaaaatg taccaaaggg gctggagggg taactcagtg gttaagaaca gtagctgctc 
    15961 ttccagagga cctgggttca attcccagca cccacatggc agcttgcaac tatctgtaac 
    16021 tgtccagctt tgggatatcc gacaccatta taccaattac ataaaatgaa aaaattgtaa 
    16081 aatgtgctaa aatgagtggg gagggggagc atggtgtggt tctgtgggct tgggattttg 
    16141 ccatgaatcc atgactatat ctcagcaata tgtggattat ttctggaaac ctgtggggca 
    16201 tgggtgtatg ggaagagggg agagagagag agcccgcatc cagccagagt tcctgtgctc 
    16261 tgggcaggcg gacgtgggag gactgctgga cgatttccac ttggccctgg gtgggcatct 
    16321 ggctgtgtga agccactgac cccactcagc ggggggtaga taaggggcat ttcccatacc 
    16381 aggttctcag actccagcat tccacgctgg atacagcaga agagctgaga acagaatagg 
    16441 ggccccaggg agacactctc gtcccagaga taaagaaggc agagggagag agattcctac 
    16501 gcaggcgaga ggccttagtc tgggccttga tgaacagaga cggtctatgg ttttagagct 
    16561 ttattgtaga aaggcagggg gaaagagaga aggtagaaag agagagacca gccatggcca 
    16621 agaggagaga aggggggaaa ggagagagag aagaaaggct agagagtaag aaaggttaaa 
    16681 gagacagaag taaaagctta gagtaagaga gagagagaga gcaagaggga gagtaagaaa 
    16741 agtaaaagct taagagagcg aggaggggcc agcccctcta atagtgggct tgttatcttg 
    16801 ttgttgctag gtaactgtga agcgtctagc ctgaaggcca gaagcttggg acattgtcta 
    16861 tgtgactgaa agccatgagc ctctactgtg ggggctgtgg aggtggtaac ttggacagga 
    16921 gccaggggtt ccaggagaca tgagggaatg ccttctgtcc catgtaggta ggaattacca 
    16981 ccaccggctt tcaccgtagt tcactggaga ctgtttgtac atagcccatt gccccacaga 
    17041 aaccaggcac tcactgtttc atttgcataa gaattagaag gctttatttt ggctgggatt 
    17101 ggtgacttgc cccagaaatt ccagcttttg ggaagccgaa tcctggagaa ccaggagttc 
    17161 aaggtcatcc tcaggtacct catttgttca agaccagcct gtgctaccat gagaccttgt 
    17221 ccctactccc ctcaacccca aaacacgctg ttttcatttt ataggttgta ctaaccatgg 
    17281 aggatggtca tttgcctttc tcttgatttc agaagcgtag cgcgggcttt gcaccatgat 
    17341 ctagtgtttc cagataggct tgcctctcca cagccaggct tatcctgctt cagtcccatg 
    17401 tttggcaagg gcaacatatg actgaagaac ttgcatagcc ttatggtgta tcatgactct 
    17461 tagccccagt ttataactgt ctaacagctg gtggcttttg tgaaaaagga aagcatcatt 
    17521 tgaactttta acttttcagc tgtttgtttt gactttgcat aaatactgag actagctagg 
    17581 aaatgagctt ttgtttcatt tgctaaaaat gatttttttt tcatgaggca atttcaagca 
    17641 atttgaattt agcaatctct acttgtctga aaagaagtta ttaattaaaa aaaaacaaac 
    17701 aaacaggaac ctattatgct ggctagtcac atgaaagaat ccaaatgaac acagtcaagg 
    17761 ctgaatcaac acatgaaatt attttctaat tccccaaatt acatttatgc taattagtat 
    17821 gtagtaaaac attttaattg tttccaatgt taaacattac agaaataatt ttgatgtttt 
    17881 cctaataacc cagcctttga tcatgtttgc atttatttga ttgttgtcag tttattaaat 
    17941 aattaagtaa gagcaacaag ataaaatgac ttcatgagag tccttttctg taacttagaa 
    18001 cgtcagtagt cataggaact gcggtcgttc ccttaccttt cagaagtagg ctaaagagtt 
    18061 gaacgcaaag gtggtttttc gaatcttcct gatccttaag cccccactag gtttatattc 
    18121 tatatttgat atcggattcg aacccctcga gcttaggatc cggaaccctt aatataacta 
    18181 agttattagg tccctcgaag aggttcacta gtactggcca ttgcggccgc ttcgaggctg 
    18241 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
    18301 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
    18361 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac 
    18421 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 
    18481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 
    18541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
    18601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
    18661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
    18721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
    18781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
    18841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
    18901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
    18961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
    19021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
    19081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
    19141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
    19201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
    19261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
    19321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
    19381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
    19441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
    19501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
    19561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
    19621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
    19681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
    19741 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
    19801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
    19861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
    19921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
    19981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
    20041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
    20101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
    20161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
    20221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
    20281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
    20341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
    20401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgg 
//
