LOCUS       pWPT-ACC/ACC/ACC       10768 bp    DNA     circular     17-OCT-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|676158344|
COMMENT     VNTDBDATE|676158344|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry|
COMMENT     VNTAUTHORNAME|Josh|
FEATURES             Location/Qualifiers
     misc_feature    17..69
                     /vntifkey="21"
                     /label=TIS:\\ACC/ACC/ACC/ACC
     CDS             64..792
                     /vntifkey="4"
                     /label=mEGFP
     RBS             820..1395
                     /vntifkey="32"
                     /label=IRES
                     /note="Internal Ribosome Entry Site (IRES)"
     CDS             1423..2133
                     /vntifkey="4"
                     /label=mCherry
     promoter        10500..10747
                     /vntifkey="29"
                     /label=EF1-short
     CDS             4048..4908
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    5673..5673
                     /vntifkey="21"
                     /label=ORI
     promoter        5962..6217
                     /vntifkey="30"
                     /label=SV40
                     /note="SV40 promoter/enhancer"
     misc_feature    6442..6899
                     /vntifkey="21"
                     /label=gpt
     polyA_signal    7301..8150
                     /vntifkey="25"
                     /label=pA
                     /note="SV40 pA and intron"
     LTR             8319..8952
                     /vntifkey="19"
                     /label=LTR
     misc_feature    9004..9141
                     /vntifkey="21"
                     /label=psi
                     /note="HIV packaging signal"
     splicing_signal 9061..9064
                     /vntifkey="38"
                     /label=SD
                     /note="5' splice signal"
     LTR             2758..3116
                     /vntifkey="19"
                     /label=LTR/SIN
                     /note="HIV LTR with U3 deletion"
     misc_recomb     2878..2912
                     /vntifkey="86"
                     /label=LoxP
                     /note="LoxP"
     misc_feature    9632..9836
                     /vntifkey="21"
                     /label=RRE
     splicing_signal 10228..10230
                     /vntifkey="38"
                     /label=SA
     primer_bind     10350..10467
                     /vntifkey="28"
                     /label=cPPT
                     /note="central PPT of HIV"
     enhancer        2142..2740
                     /vntifkey="9"
                     /label=WPRE
BASE COUNT     2768 a      2683 c      2768 g      2549 t 
ORIGIN
        1 tcgagtaact aactaaacca tgggttgata aaccatgggt tgataaacca tgggttgatt 
       61 accatgggcg aattcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
      121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
      181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 
      241 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 
      301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
      361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
      421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
      481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 
      541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 
      601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
      661 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat 
      721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 
      781 tacaagttcg aataagcatg catctagggc ggccaattcc gcccctctcc ctcccccccc 
      841 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtga 
      901 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
      961 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     1021 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     1081 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     1141 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     1201 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     1261 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 
     1321 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     1381 aaaacacgat gataagcttg ccacaacccg ggatccgcca ccatggtgag caagggcgag 
     1441 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 
     1501 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 
     1561 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 
     1621 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 
     1681 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 
     1741 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 
     1801 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 
     1861 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 
     1921 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 
     1981 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 
     2041 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 
     2101 tccaccggcg gcatggacga gctgtacaag taagcggccg ccgataatca acctctggat 
     2161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     2221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     2281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     2341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     2401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     2461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     2521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 
     2581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     2641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     2701 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg caattcgagc tcggtacctt 
     2761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 
     2821 gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat 
     2881 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc 
     2941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 
     3001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 
     3061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag 
     3121 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta 
     3181 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg 
     3241 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg 
     3301 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa 
     3361 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg 
     3421 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 
     3481 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 
     3541 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 
     3601 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 
     3661 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 
     3721 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 
     3781 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 
     3841 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 
     3901 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     3961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     4021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     4081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     4141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     4201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     4261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     4321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     4381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     4441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     4501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     4561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     4621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     4681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     4741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     4801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     4861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     4921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     4981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     5221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     5461 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     5761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
     5821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 
     5881 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 
     5941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 
     6001 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     6061 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     6121 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     6181 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 
     6241 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg 
     6301 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg 
     6361 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat 
     6421 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag 
     6481 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt 
     6541 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc 
     6601 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc 
     6661 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg 
     6721 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg 
     6781 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct 
     6841 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat 
     6901 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag 
     6961 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg 
     7021 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc 
     7081 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg 
     7141 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag 
     7201 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt 
     7261 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta 
     7321 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa 
     7381 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 
     7441 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa 
     7501 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 
     7561 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 
     7621 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta 
     7681 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac 
     7741 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag 
     7801 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat 
     7861 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca 
     7921 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 
     7981 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 
     8041 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     8101 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 
     8161 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 
     8221 acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 
     8281 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca 
     8341 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta 
     8401 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 
     8461 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt 
     8521 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 
     8581 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa 
     8641 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg 
     8701 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt 
     8761 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 
     8821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 
     8881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 
     8941 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct 
     9001 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact 
     9061 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag 
     9121 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg 
     9181 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt 
     9241 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct 
     9301 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac 
     9361 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat 
     9421 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc 
     9481 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta 
     9541 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga 
     9601 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc 
     9661 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 
     9721 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc 
     9781 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag 
     9841 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg 
     9901 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg 
     9961 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa 
    10021 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt 
    10081 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata 
    10141 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt 
    10201 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac 
    10261 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta 
    10321 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac 
    10381 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 
    10441 aaacaaatta caaaaattca aaattttccg atcacgagac tagcctcgac aagcttgatc 
    10501 gatggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 
    10561 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa 
    10621 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 
    10681 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg 
    10741 tgacgcgcat ccaggcctaa gcttacgc 
//
