LOCUS DS-SPcas 6806 bp DNA circular 30-JAN-2013 DEFINITION . SOURCE . ORGANISM . COMMENT ApEinfo:methylated:1 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|652374716| COMMENT VNTDBDATE|652374716| COMMENT LSOWNER| COMMENT VNTNAME|DS-SPcas| COMMENT VNTAUTHORNAME|Kevin Esvelt| COMMENT VNTAUTHOREML|kesvelt@gmail.com| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT #144=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #82# 617 100) COMMENT (LOGPEN 0 6 13395507) 10 1 1.70978 1.9 COMMENT 0.082322 1) COMMENT #145=(CLabel (CWidget 0 (0 0) 1 2 0 0 #74# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "terminator" COMMENT "@N" 1 0 0 8.3017 5.99088 3.4359 0.820513 COMMENT #144#)) (CObjectList)) COMMENT #146=(CGroupWidget (CWidget 55 (7 55 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #147=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #78# 959 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.00012 COMMENT 2.08215 1 0.082322) 0.8 1.8 0) COMMENT #148=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #74# -1239 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 COMMENT "tracrRNA precursor" "@N" 1 0 0 8.3037 COMMENT 12.3243 6.48718 0.820513 #147#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #149=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 16646296 100) COMMENT (CObjectList) (CObjectList)) COMMENT #150=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 22741032 100) COMMENT (CObjectList) (CObjectList)) COMMENT #151=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -105003360 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers terminator 107..194 /vntifkey="43" /label=rrnBT2\term /note="near-perfect bidirectional terminator" promoter 205..364 /vntifkey="30" /label=proC precursor_RNA 4548..4636 /vntifkey="55" /label=tracrRNA\precursor promoter 4488..4541 /vntifkey="30" /label=tracrRNA\promoter terminator 4615..4636 /vntifkey="43" /label=tracrRNA\terminator terminator complement(4667..4708) /vntifkey="43" /label=terminator /note="from S. pyogenes near tracrRNA; probably bidirectional but unconfirmed" CDS 377..4483 /vntifkey="4" /label=cas9 rep_origin 5062..5800 /vntifkey="33" /label=cloD13\(CDF) CDS 5940..6728 /vntifkey="4" /label=aadA /note="spectinomycin resistance" misc_feature 4845..4880 /vntifkey="21" /label=repeat /note="SP repeat" promoter 4765..4833 /vntifkey="30" /label=J23100\promoter terminator 4952..4982 /vntifkey="43" /label=terminator BASE COUNT 2144 a 1273 c 1531 g 1858 t ORIGIN 1 accaggcacg cctaaccgtc agtgagattg gatgagtgaa cgatattgat cgagaagagc 61 cctgcgcagc cgctgccgtg cctgcaggaa gcaacggccc ggagggtggc gggcaggacg 121 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 181 tgcgtttcta caaactctgc tagcttctag agcacagcta acaccacgtc gtccctatct 241 gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcgtat 301 gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aacttttact 361 agaggaggag gcaaaaatgg ataagaaata ctcaataggc ttagatatcg gcacaaatag 421 cgtcggatgg gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct 481 gggaaataca gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag 541 tggagagaca gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg 601 gaagaatcgt atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga 661 tagtttcttt catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg 721 tcatcctatt tttggaaata tagtagatga agttgcttat catgagaaat atccaactat 781 ctatcatctg cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta 841 tttggcctta gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa 901 tcctgataat agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt 961 atttgaagaa aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg 1021 attgagtaaa tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa 1081 tggcttattt gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa 1141 ttttgatttg gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt 1201 agataattta ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa 1261 tttatcagat gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc 1321 tcccctatca gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt 1381 aaaagcttta gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc 1441 aaaaaacgga tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt 1501 tatcaaacca attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg 1561 tgaagatttg ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca 1621 cttgggtgag ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga 1681 caatcgtgag aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt 1741 ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc 1801 atggaatttt gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat 1861 gacaaacttt gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta 1921 tgagtatttt acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg 1981 aaaaccagca tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac 2041 aaatcgaaaa gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt 2101 tgatagtgtt gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca 2161 tgatttgcta aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat 2221 cttagaggat attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag 2281 acttaaaaca tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg 2341 ttatactggt tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc 2401 tggcaaaaca atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca 2461 gctgatccat gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg 2521 acaaggcgat agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa 2581 aggtatttta cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa 2641 gccagaaaat atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa 2701 aaattcgcga gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat 2761 tcttaaagag catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta 2821 tctccaaaat ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga 2881 ttatgatgtc gatcacattg ttccacaaag tttccttaaa gacgattcaa tagacaataa 2941 ggtcttaacg cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt 3001 agtcaaaaag atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg 3061 taagtttgat aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg 3121 ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt 3181 ggatagtcgc atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt 3241 gattacctta aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt 3301 acgtgagatt aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac 3361 tgctttgatt aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt 3421 ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa 3481 atatttcttt tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg 3541 agagattcgc aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga 3601 taaagggcga gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt 3661 caagaaaaca gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa 3721 ttcggacaag cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga 3781 tagtccaacg gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa 3841 gaagttaaaa tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga 3901 aaaaaatccg attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat 3961 cattaaacta cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc 4021 tagtgccgga gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt 4081 tttatattta gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa 4141 acaattgttt gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga 4201 attttctaag cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa 4261 caaacataga gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt 4321 gacgaatctt ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg 4381 atatacgtct acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct 4441 ttatgaaaca cgcattgatt tgagtcagct aggaggtgac tgaccggctg ataaatttct 4501 ttgaatttct ccttgattat ttgttataaa agttataaaa taatcttgtt ggaaccattc 4561 aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 4621 agtcggtgct ttttttgata cttctattct actctgactg caaaccaaaa aaacaagcgc 4681 tttcaaaacg cttgttttat catttttagg gaaattaatc tcttaatcct tttatcattc 4741 tacatttagg cgctgccatc ttgggacaat gaaaacgtta gtcatggcgc gccttgacgg 4801 ctagctcagt cctaggtaca gtgctagctt aattagtcta cgaggtttta gagctatgct 4861 gttttgaatg gtcccaaaac ctgcagacaa gcccggccgg cctaaggcga tgccccctcg 4921 acctcgatca gggaggcgtt caggacgact cacaaagaaa gccgggcaat gcccggcttt 4981 ttccacgcct cctgggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat 5041 ctagagcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 5101 aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt 5161 cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca accaaatctg 5221 tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg tttagctaaa 5281 caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt taccgtgctt 5341 ttccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg 5401 gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg 5461 tgtgagctat gagaaagcgc cacacttccc gtaagggaga aaggcggaac aggtatccgg 5521 taaacggcag ggtcggaaca ggagagcgca agagggagcg acccgccgga aacggtgggg 5581 atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct 5641 cccacagatg caccggaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta 5701 tggaaaaacg ccaccggcgc ggccctgctg ttttgcctca catgttagtc ccctgcttat 5761 ccacggaatc tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg 5821 gagcgtagcg accgagtgag ctagctattt gtttattttt ctaaatacat tcaaatatgt 5881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 5941 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 6001 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 6061 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 6121 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 6181 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 6241 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 6301 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 6361 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 6421 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 6481 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 6541 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 6601 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 6661 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag 6721 tcggcaaata atgtctaaca attcgttcaa gccgaggggc cgcaagatcc ggccacgatg 6781 acccggtcgt cggttcaggg cagggt //