LOCUS       pCAG-Co-InCreC      5811 bp    DNA     circular 
FEATURES             Location/Qualifiers
     Promoter        14..1681
                     /label=CAG Promoter
     Promoter        1691..1713
                     /label=T7
     CDS             1729..2718
                     /label=Co-InCreC
     split-intein    1732..1842
                     /label=C-gp41-1
     misc            1843..2694
                     /label=iCre_aa60-343
     Signal_peptide  2695..2715
                     /label=SV40 NLS
     PolyA_signal    2726..2996
                     /label=BGHpA
     CDS             3784..4644
                     /label=Amp
ORIGIN
        1 GCGCGCCGGA TTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA
       61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
      121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
      181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
      241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
      301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
      361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC
      421 ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA
      481 TTTTGTGCAG CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG
      541 GGCGAGGGGC GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG
      601 CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC
      661 GCGCGGCGGG CGGGAGTCGC TGCGTTGCCT TCGCCCCGTG CCCCGCTCCG CGCCGCCTCG
      721 CGCCGCCCGC CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC
      781 CCTTCTCCTC CGGGCTGTAA TTAGCGCTTG GTTTAATGAC GGCTCGTTTC TTTTCTGTGG
      841 CTGCGTGAAA GCCTTAAAGG GCTCCGGGAG GGCCCTTTGT GCGGGGGGGA GCGGCTCGGG
      901 GGGTGCGTGC GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC GGCCCGCGCT GCCCGGCGGC
      961 TGTGAGCGCT GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC GCGTGTGCGC GAGGGGAGCG
     1021 CGGCCGGGGG CGGTGCCCCG CGGTGCGGGG GGGCTGCGAG GGGAACAAAG GCTGCGTGCG
     1081 GGGTGTGTGC GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG GCGGTCGGGC TGTAACCCCC
     1141 CCCTGCACCC CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC TTCGGGTGCG GGGCTCCGTG
     1201 CGGGGCGTGG CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG
     1261 GCGGGGCGGG GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGCCCCGGAG
     1321 CGCCGGCGGC TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA
     1381 GAGGGCGCAG GGACTTCCTT TGTCCCAAAT CTGGCGGAGC CGAAATCTGG GAGGCGCCGC
     1441 CGCACCCCCT CTAGCGGGCG CGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG
     1501 GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCATCTCCAG CCTCGGGGCT
     1561 GCCGCAGGGG GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG
     1621 TGTGACCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA
     1681 GATCCTTAAT TAATAATACG ACTCACTATA GGGGGTCGAC CCGCCACCAT GATGCTGAAG
     1741 AAGATCCTGA AGATCGAGGA GCTGGACGAG AGAGAGCTGA TCGACATCGA GGTGAGCGGC
     1801 AACCACCTGT TCTACGCCAA CGACATCCTG ACCCACAACA GCAACAGGAA ATGGTTCCCT
     1861 GCTGAACCTG AGGATGTGAG GGACTACCTC CTGTACCTGC AAGCCAGAGG CCTGGCTGTG
     1921 AAGACCATCC AACAGCACCT GGGCCAGCTC AACATGCTGC ACAGGAGATC TGGCCTGCCT
     1981 CGCCCTTCTG ACTCCAATGC TGTGTCCCTG GTGATGAGGA GAATCAGAAA GGAGAATGTG
     2041 GATGCTGGGG AGAGAGCCAA GCAGGCCCTG GCCTTTGAAC GCACTGACTT TGACCAAGTC
     2101 AGATCCCTGA TGGAGAACTC TGACAGATGC CAGGACATCA GGAACCTGGC CTTCCTGGGC
     2161 ATTGCCTACA ACACCCTGCT GCGCATTGCC GAAATTGCCA GAATCAGAGT GAAGGACATC
     2221 TCCCGCACCG ATGGTGGGAG AATGCTGATC CACATTGGCA GGACCAAGAC CCTGGTGTCC
     2281 ACAGCTGGTG TGGAGAAGGC CCTGTCCCTG GGGGTTACCA AGCTGGTGGA GAGATGGATC
     2341 TCTGTGTCTG GTGTGGCTGA TGACCCCAAC AACTACCTGT TCTGCCGGGT CAGAAAGAAT
     2401 GGTGTGGCTG CCCCTTCTGC CACCTCCCAA CTGTCCACCC GGGCCCTGGA AGGGATCTTT
     2461 GAGGCCACCC ACCGCCTGAT CTATGGTGCC AAGGATGACT CTGGGCAGAG ATACCTGGCC
     2521 TGGTCTGGCC ACTCTGCCAG AGTGGGTGCT GCCAGGGACA TGGCCAGGGC TGGTGTGTCC
     2581 ATCCCTGAAA TCATGCAGGC TGGTGGCTGG ACCAATGTGA ACATTGTGAT GAACTACATC
     2641 AGAAACCTGG ACTCTGAGAC TGGGGCCATG GTGAGGCTGC TCGAGGATGG GGACCCCAAG
     2701 AAGAAGAGGA AGGTGTAGgt caGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
     2761 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
     2821 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
     2881 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
     2941 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCG
     3001 AGATCCACTA GTTCTAGCCT CGAGGCTAGA GCGGCCGCCA CTGGCCGTCG TTTTACAACG
     3061 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     3121 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
     3181 CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT
     3241 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT
     3301 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC
     3361 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA
     3421 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC
     3481 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT
     3541 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT
     3601 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC
     3661 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT
     3721 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG
     3781 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT
     3841 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT
     3901 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC
     3961 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA
     4021 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC
     4081 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA
     4141 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
     4201 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
     4261 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
     4321 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
     4381 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
     4441 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
     4501 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
     4561 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
     4621 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT
     4681 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     4741 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     4801 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     4861 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     4921 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     4981 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     5041 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     5101 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     5161 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     5221 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     5281 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     5341 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     5401 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     5461 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA
     5521 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG
     5581 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC
     5641 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT
     5701 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT
     5761 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA G
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