LOCUS       8N5C_pDisplay2_Ann        7558 bp ds-DNA   circular     29-AUG-2008
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    2410..2559
                     /label=PDGFR 513-561
                     /ApEinfo_fwdcolor=#a58d2a
                     /ApEinfo_revcolor=#a58d2a
     primer_bind     637..656
                     /label=T7 promoter/priming site
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(2922..3604)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     rep_origin      complement(6371..6827)
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(5382..6041)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    736..798
                     /label=Ig K-Chain
                     /ApEinfo_fwdcolor=#845300
                     /ApEinfo_revcolor=green
     misc_feature    2377..2406
                     /label=myc_epi
                     /ApEinfo_fwdcolor=#fe84ff
                     /ApEinfo_revcolor=green
     misc_feature    complement(2578..2595)
                     /label=r.0.401
                     /ApEinfo_fwdcolor=#8180ff
                     /ApEinfo_revcolor=#8180ff
     misc_feature    799..804
                     /note="MCS"
     misc_feature    805..1551
                     /note="YbeJ5-253"
     misc_feature    1552..1827
                     /note="cpGFP 147-238"
     misc_feature    1828..1845
                     /note="Linker"
     misc_feature    1846..2286
                     /note="cpGFP 1-146"
     misc_feature    2287..2343
                     /note="YbeJ 254-272"
     misc_feature    2344..2364
                     /note="YbeJ %C6272-280"
     misc_feature    2..597
                     /note="hCMV promoter/enhancer"
     misc_feature    545..549
                     /note="CAAAT"
     misc_feature    578..584
                     /note="TATA box"
ORIGIN      
          1 CGCGCGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT
         61 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG
        121 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC
        181 AATAGGGACT TTCCATTGAC GTCAATGGGT GGACTATTTA CGGTAAACTG CCCACTTGGC
        241 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG
        301 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT
        361 CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG
        421 TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG
        481 TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT
        541 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAA CTCTCTGGCT
        601 AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT ATAGGGAGAC
        661 CCAAGCTTGG TACCGAACTC GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCGGC
        721 TTGGGGATAT CCACCATGGA GACAGACACA CTCCTGCTAT GGGTACTGCT GCTCTGGGTT
        781 CCAGGTTCCA CTGGTGACAG ATCTgccgca ggcagcacgc tggacaaaat cgccaaaaac
        841 ggtgtgattg tcgtcggtca ccgtgaatct tcagtgcctt tctcttatta cgacaatcag
        901 caaaaagtgg tgggttactc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag
        961 aaactcaaca aaccggactt gcaggtaaaa ctgattccga ttacctcaca aaaccgtatt
       1021 ccactgctgc aaaacggcac tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa
       1081 cgccaaaaac aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgttgacc
       1141 aaaaagggtg gcgatatcaa agattttgcc aacctgaaag acaaagccgt agtcgtcact
       1201 tccggcacta cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg
       1261 cgcatcatca gcgccaaaga tcacggtgac tctttccgca ccctggaaag cggtcgtgcc
       1321 gttgccttta tgatggatga cGTGctgctg gccggtgaac gtgcgaaagc gaagaaacca
       1381 gacaactggg aaatcgtcgg caagccgcag tctcaggagg cctacggttg tatgttgcgt
       1441 aaagatgatc cgcagttcaa aaagctgatg gatgacacca tcgctcaggt gcagacctcc
       1501 ggtgaagcgg aaaaatggtt tgataagtgg ttcaaaaatc caattctggt gAGCCACAAC
       1561 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG CGAACTTCAA GATCCGCCAC
       1621 AACATCGAGG ACGGCGGCGT GCAGCTCGCC TACCACTACC AGCAGAACAC CCCCATCGGC
       1681 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA ACTTTCGAAA
       1741 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
       1801 ACTCTCGGCA TGGACGAGCT GTACAAGGGC GGTACCGGAG GGAGCATGGT GAGCAAGGGC
       1861 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
       1921 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
       1981 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
       2041 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
       2101 AAGTCCGCCA TGCCCGAAGG CTACATCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
       2161 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
       2221 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCATAAGCT GGAGTACAAC
       2281 TTTAACaaCC Ccctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa
       2341 gaaccgaatg acaaggcact gaaaCTGCAG GTCGACGAAC AAAAACTCAT CTCAGAAGAG
       2401 GATCTGAATG CTGTGGGCCA GGACACGCAG GAGGTCATCG TGGTGCCACA CTCCTTGCCC
       2461 TTTAAGGTGG TGGTGATCTC AGCCATCCTG GCCCTGGTGG TGCTCACCAT CATCTCCCTT
       2521 ATCATCCTCA TCATGCTTTG GCAGAAGAAG CCACGTTAGG CGGCCGCTCG AGATCAGCCT
       2581 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
       2641 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
       2701 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
       2761 ATTGGGAAGA CAATAGCAGG CGTGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
       2821 AAAGAACCAG TGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
       2881 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
       2941 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
       3001 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
       3061 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
       3121 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
       3181 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
       3241 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
       3301 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
       3361 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
       3421 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
       3481 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
       3541 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
       3601 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
       3661 TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG
       3721 GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG
       3781 CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC
       3841 CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT
       3901 GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC
       3961 CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG
       4021 TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT
       4081 CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA
       4141 AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT
       4201 AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG
       4261 CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG
       4321 AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA
       4381 GATCCTCGCC GTCGGGCTTG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC
       4441 CCTGATGCTC TTGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT
       4501 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC
       4561 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA
       4621 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT
       4681 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT
       4741 CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT
       4801 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT
       4861 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA
       4921 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA
       4981 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGAGGCGGC CTCGGCCTCT
       5041 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT
       5101 AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCTTG
       5161 CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA
       5221 TTGAGATGCT TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT
       5281 AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATAAA
       5341 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
       5401 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
       5461 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
       5521 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
       5581 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
       5641 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
       5701 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
       5761 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
       5821 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
       5881 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
       5941 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
       6001 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
       6061 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
       6121 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
       6181 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
       6241 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
       6301 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
       6361 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
       6421 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
       6481 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
       6541 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
       6601 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
       6661 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
       6721 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
       6781 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTACG
//
