LOCUS pSicoR-Ef1a-mCh-Puro 8141 bp DNA circular 23-JUN-2011 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|593689317| COMMENT VNTDBDATE|593689444| COMMENT LSOWNER| COMMENT VNTNAME|pSicoR-Ef1a-mCh-Puro| COMMENT VNTAUTHORNAME|Matthew Spindler| COMMENT VNTAUTHORTEL|415-734-2833| COMMENT VNTAUTHORFAX|415-355-0960| COMMENT VNTAUTHOREML|mspindler@gladstone.ucsf.edu| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSicoR-Ef1a-mCh-Puro" 0 8141 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 6 4294967295 150 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) COMMENT (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 8 7 9 61 10 11 12 COMMENT 13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 25 26 55 91 COMMENT 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 57 38 36 39 40 41 42 43 COMMENT 95 44 59 99 96 97 45 100 46) (CWordArray) COMMENT (CStringList "EcoRI" "SalI" "BglII" "PvuII" "HpaI" "XhoI" "AgeI" "EagI" COMMENT "NotI" "NheI" "SacII" "XbaI" "XmaI" "NcoI" "MscI" "BsrGI" "BseRI" COMMENT "MluI" "AflII") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 8141 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4923 0 0 "") COMMENT #1=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 11 0 5629 0 0 "") COMMENT #2=(CRSite (CStringList) "BglII" "agatct" 2 0 4 25 0 864 0 5793 0 6167 0 COMMENT 0 "") COMMENT #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 3230 0 4133 0 5788 0 0 COMMENT "") #4=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 2944 0 0 "") COMMENT #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2959 0 0 "") COMMENT #6=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 3078 0 3542 0 0 "") COMMENT #7=(CRSite (CStringList) "EagI" "cggccg" 2 0 4 1535 0 1539 0 2982 0 4771 COMMENT 0 0 "") COMMENT #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2982 0 0 "") COMMENT #9=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 3533 0 0 "") COMMENT #10=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 2988 0 4537 0 5534 0 0 COMMENT "") #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2626 0 0 "") COMMENT #12=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4264 0 0 "") COMMENT #13=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 623 0 3553 0 3988 0 0 "") COMMENT #14=(CRSite (CStringList) "MscI" "tggcca" 4 0 2 3585 0 4698 0 0 "") COMMENT #15=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 4255 0 0 "") COMMENT #16=(CRSite (CStringList) "BseRI" "gaggag" 17 15 12 1081 0 1578 0 1581 0 COMMENT 3585 0 3890 1 3995 1 4320 0 5156 1 5217 0 5737 0 5740 0 5743 0 0 "") COMMENT #17=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 241 0 0 "") COMMENT #18=(CRSite (CStringList) "AflII" "cttaag" 2 0 3 3 0 908 0 6211 0 0 "")) COMMENT (CObList COMMENT #19=(CFSignal (CObList) "Psi" 21 0 0 1076 1213 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1076..1213") COMMENT #20=(CFSignal (CObList) "RRE" 21 0 0 1707 1910 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1707..1910") COMMENT #21=(CFSignal (CObList) "shRNA Cloning Site" 21 0 0 2944 2958 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2945..2960") COMMENT #22=(CFSignal (CObList) "U6 Promoter" 29 0 0 2631 2943 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2631..2944") COMMENT #23=(CFSignal (CObList) "loxP" 21 0 0 2825 2858 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2826..2859") COMMENT #24=(CFSignal (CObList) "loxP" 21 0 0 4941 4974 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4358..4391") COMMENT #25=(CFSignal (CObList) "WRE" 21 0 0 5030 5409 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4447..4826") COMMENT #26=(CFSignal (CObList) "mCherry" 22 0 0 3554 4261 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3628..4338") COMMENT #27=(CFSignal (CObList) "EF-1alpha promoter" 29 0 0 2989 3531 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "707..1249") COMMENT #28=(CFSignal (CObList) "Puro" 21 0 0 4325 4918 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4870..5463") COMMENT #29=(CFSignal (CObList) "T2A" 21 0 0 4268 4321 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4813..4866") COMMENT #30=(CFSignal (CObList) "AmpR" 21 0 1 7151 8008 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #31=(CFSignal (CObList) "Lox Replace Rev Primer" 27 0 1 493 512 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(493..512)") COMMENT #32=(CFSignal (CObList) "Lox Replace For Primer" 27 0 0 2481 2500 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2481..2500")) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #33=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #34=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "pSicoR-Ef1a-mCh-Puro" 2) 5 "" 0 4) COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSicoR-Ef1a-mCh-Puro'" 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 06/23/11 09:41AM" 1) COMMENT #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 06/23/11 09:44AM" 1) COMMENT #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 8141 bp" 1) COMMENT #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #43=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 12-JAN-2006" 1))) COMMENT "Standard Fields") COMMENT #46=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #47=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #48=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #49=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #50=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1076 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1076 End: 1213" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1076..1213" 1))) "Psi") COMMENT #54=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1707 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1707 End: 1910" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1707..1910" 1))) "RRE") COMMENT #58=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2825 (3 #23# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2825 End: 2858" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2826..2859" 1))) "loxP") COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2944 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2944 End: 2958" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2945..2960" 1))) COMMENT "shRNA Cloning Site") COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4268 (3 #29# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4268 End: 4321" 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4813..4866" 1))) "T2A") COMMENT #70=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4325 (3 #28# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4325 End: 4918" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4870..5463" 1))) "Puro") COMMENT #74=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4941 (3 #24# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4941 End: 4974" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4358..4391" 1))) "loxP") COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5030 (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5030 End: 5409" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4447..4826" 1))) "WRE") COMMENT #82=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 7151 (3 #30# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 7151 End: 8008 (Complementary)" COMMENT 1))) "AmpR"))) "Misc. Feature (9 total)") COMMENT #84=(CFolderPar COMMENT (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #85=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3554 (3 #26# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3554 End: 4261" 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3628..4338" 1))) "mCherry"))) COMMENT "Misc. Marker (1 total)") COMMENT #89=(CFolderPar COMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #90=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 493 (3 #31# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 493 End: 512 (Complementary)" COMMENT 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(493..512)" 1))) COMMENT "Lox Replace Rev Primer") COMMENT #94=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2481 (3 #32# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2481 End: 2500" 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2481..2500" 1))) COMMENT "Lox Replace For Primer"))) "Primer (2 total)") COMMENT #98=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #99=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2631 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2631 End: 2943" 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2631..2944" 1))) "U6 Promoter") COMMENT #103=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2989 (3 #27# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2989 End: 3531" 1) COMMENT #105=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 707..1249" 1))) COMMENT "EF-1alpha promoter"))) COMMENT "Promoter Eukaryotic (2 total)"))) "Feature Map") COMMENT #107=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #108=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42772344 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "AflII: 3 sites") 1 5 "CTTAAG" COMMENT "GAATTC") COMMENT #109=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639656 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #110=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #111=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 3078 " 1) COMMENT #112=(CLinePar COMMENT (CParagraph 0 (1 #6# 2) 1 2 2 0 191) COMMENT " N2: 3542 " 1))))) "AgeI: 2 sites") COMMENT 1 5 "ACCGGT" "TGGCCA") COMMENT #113=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639784 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BglII: 4 sites") 1 5 "AGATCT" COMMENT "TCTAGA") COMMENT #114=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42772280 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BseRI: 12 sites") 16 14 COMMENT "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") COMMENT #115=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42772248 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #116=(CGroupPar COMMENT (CParagraph 0 (10 #15# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #117=(CLinePar COMMENT (CParagraph 0 (1 #15# 1) 1 2 2 0 191) COMMENT " N1: 4255 " 1))))) "BsrGI: 1 site") COMMENT 1 5 "TGTACA" "ACATGT") COMMENT #118=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639624 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "EagI: 4 sites") 1 5 "CGGCCG" COMMENT "GCCGGC") COMMENT #119=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639848 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #120=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 4923 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #122=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639720 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #123=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #124=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 2944 " 1))))) "HpaI: 1 site") COMMENT 3 3 "GTTAAC" "CAATTG") COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42772312 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #126=(CGroupPar COMMENT (CParagraph 0 (10 #17# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (1 #17# 1) 1 2 2 0 191) COMMENT " N1: 241 " 1))))) "MluI: 1 site") 1 COMMENT 5 "ACGCGT" "TGCGCA") COMMENT #128=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639880 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #129=(CGroupPar COMMENT (CParagraph 0 (10 #14# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #130=(CLinePar COMMENT (CParagraph 0 (1 #14# 1) 1 2 2 0 191) COMMENT " N1: 3585 " 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (1 #14# 2) 1 2 2 0 191) COMMENT " N2: 4698 " 1))))) "MscI: 2 sites") COMMENT 3 3 "TGGCCA" "ACCGGT") COMMENT #132=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42640048 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" COMMENT "GGTACC") COMMENT #133=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639424 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #134=(CGroupPar COMMENT (CParagraph 0 (10 #9# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #135=(CLinePar COMMENT (CParagraph 0 (1 #9# 1) 1 2 2 0 191) COMMENT " N1: 3533 " 1))))) "NheI: 1 site") COMMENT 1 5 "GCTAGC" "CGATCG") COMMENT #136=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639592 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #137=(CGroupPar COMMENT (CParagraph 0 (10 #8# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #138=(CLinePar COMMENT (CParagraph 0 (1 #8# 1) 1 2 2 0 191) COMMENT " N1: 2982 " 1))))) "NotI: 1 site") COMMENT 2 6 "GCGGCCGC" "CGCCGGCG") COMMENT #139=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639752 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "PvuII: 3 sites") 3 3 "CAGCTG" COMMENT "GTCGAC") COMMENT #140=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42640280 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SacII: 3 sites") 4 2 "CCGCGG" COMMENT "GGCGCC") COMMENT #141=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639816 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #142=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #143=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 11 " 1) COMMENT #144=(CLinePar COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT " N2: 5629 " 1))))) "SalI: 2 sites") COMMENT 1 5 "GTCGAC" "CAGCTG") COMMENT #145=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42640248 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #146=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #147=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT " N1: 2626 " 1))))) "XbaI: 1 site") COMMENT 1 5 "TCTAGA" "AGATCT") COMMENT #148=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42639688 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #149=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #150=(CLinePar COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191) COMMENT " N1: 2959 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC") COMMENT #151=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 42640080 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #152=(CGroupPar COMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #153=(CLinePar COMMENT (CParagraph 0 (1 #12# 1) 1 2 2 0 191) COMMENT " N1: 4264 " 1))))) "XmaI: 1 site") COMMENT 1 5 "CCCGGG" "GGGCCC"))) COMMENT "Restriction/Methylation Map: (no more than 2 sites)")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #154=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #155=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #156=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #157=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #158=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #159=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #160=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #161=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #162=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #163=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #164=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #165=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #166=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #167=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #168=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #169=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #170=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #171=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #172=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #173=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #174=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #175=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #176=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #177=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #178=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #179=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #180=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #181=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #182=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #183=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #184=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #185=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #186=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #187=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #188=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #189=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #190=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #191=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #192=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #193=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -8141) 1 1 0 1 1 COMMENT (mapper: 17.7486 -14.367 40.0378 40.0378 0.01 10 -1 8141 8141 1 0 0) COMMENT #194=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -2060 100) COMMENT (CObjectList COMMENT #195=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -870 100) COMMENT (CObjectList COMMENT #196=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #160# 39090596 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27496 6.27296 1 0.0214037) 0.0770567) COMMENT #197=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #157# 1114134 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSicoR-Ef1a-mCh-Puro" "@N" 1 0 COMMENT 0.871165 0 -10 13.3873 1.39868 Nil) COMMENT #198=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #156# 37620572 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "8141 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.42271 0.799245 Nil)) (CObjectList)) COMMENT #199=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1428 100) COMMENT (CObjectList COMMENT #200=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -1358 100) COMMENT (CObjectList COMMENT #201=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 5.33849 5.44483 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #202=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 24396572 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Psi" "@N" 1 COMMENT 10.7806 6.96932 0 -10 1.49858 1.04901 #201#) COMMENT #203=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 4.8014 4.9586 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #204=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 1649361704 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "RRE" "@N" 1 COMMENT 12.8143 1.74233 0 -10 1.94816 1.04901 #203#) COMMENT #205=(CScratch COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #163# 932 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.00541 1.9 COMMENT 0.082322 1) COMMENT #206=(CLabel (CWidget 0 (0 0) 1 2 0 0 #155# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "shRNA Cloning Site" "@N" 1 14.274 -6.96932 0 COMMENT -10 8.44202 1.04901 #205#) COMMENT #207=(CScratch COMMENT (CWidget 0 (3 #23# 0) 1 2 0 0 #163# 579 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.0971 1.9 COMMENT 0.082322 1) COMMENT #208=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 1944545584 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT 11.9587 -5.22699 0 -10 2.02309 1.04901 #207#) COMMENT #209=(CScratch COMMENT (CWidget 0 (3 #24# 0) 1 2 0 0 #163# 30714616 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.46658 1.9 COMMENT 0.082322 1) COMMENT #210=(CLabel (CWidget 0 (0 0) 1 2 0 0 #155# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT -12.5307 -3.48466 0 -10 2.02309 1.04901 #209#) COMMENT #211=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #25# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.10519 COMMENT 2.398 1 0.082322) 0.8 1.8 0) COMMENT #212=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 3670432 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "WRE" "@N" 1 COMMENT -12.9101 -1.74233 0 -10 2.12299 1.04901 #211#) COMMENT #213=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #28# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.48354 COMMENT 2.94125 1 0.082322) 0.8 1.8 0) COMMENT #214=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 1129595207 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Puro" "@N" 1 COMMENT -9.77328 -8.71165 0 -10 2.12299 1.04901 #213#) COMMENT #215=(CScratch COMMENT (CWidget 0 (3 #29# 0) 1 2 0 0 #163# 1003 100) COMMENT (LOGPEN 0 8 13395507) 10 1 2.98518 1.9 COMMENT 0.082322 1) COMMENT #216=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 29758364 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T2A" "@N" 1 COMMENT -7.36209 -10.454 0 -10 1.67342 1.04901 #215#) COMMENT #217=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #30# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 0.102485 0.763632 COMMENT 1 0.082322) 0.8 1.8 1) COMMENT #218=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 1114122 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -7.82381 10.454 0 -10 2.6475 1.04901 #217#)) COMMENT (CObjectList)) COMMENT #219=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -591 100) COMMENT (CObjectList COMMENT #220=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #26# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 2.9898 3.53536 1 0.082322) 0.8 1.8 0) COMMENT #221=(CLabel (CWidget 0 (0 0) 1 2 0 0 #155# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "mCherry" "@N" 1 COMMENT 4.50227 -22.6503 0 -10 3.69651 1.04901 #220#)) COMMENT (CObjectList)) COMMENT #222=(CGroupWidget COMMENT (CWidget 27 (7 27 0) 1 2 0 0 Nil -102 100) COMMENT (CObjectList COMMENT #223=(CScratch COMMENT (CWidget 0 (3 #31# 0) 1 2 0 0 #163# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.89407 1.9 COMMENT 0.082322 1) COMMENT #224=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 31345260 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Lox Replace Rev Primer" "@N" 1 13.8242 COMMENT 8.71165 0 -10 10.6399 1.04901 #223#) COMMENT #225=(CScratch COMMENT (CWidget 0 (3 #32# 0) 1 2 0 0 #163# 449 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.36218 1.9 COMMENT 0.082322 1) COMMENT #226=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 30101484 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Lox Replace For Primer" "@N" 1 17.0285 0 0 COMMENT -10 10.2903 1.04901 #225#)) (CObjectList)) COMMENT #227=(CGroupWidget COMMENT (CWidget 29 (7 29 0) 1 2 0 0 Nil -1149 100) COMMENT (CObjectList COMMENT #228=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.00541 COMMENT 4.24659 1 0.082322) 0.8 1.8 0) COMMENT #229=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 24566260 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "U6 Promoter" COMMENT "@N" 1 14.273 -3.48466 0 -10 5.46983 1.04901 COMMENT #228#) COMMENT #230=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #27# 0) 1 2 0 0 #159# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.55231 COMMENT 3.97073 1 0.082322) 0.8 1.8 0) COMMENT #231=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #155# 44812508 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "EF-1alpha promoter" "@N" 1 12.2277 -15.681 0 COMMENT -10 8.54193 1.04901 #230#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #232=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -172 100) COMMENT (CObjectList COMMENT #233=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1149 100) COMMENT (CObjectList COMMENT #234=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #162# 895 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.48045 1.9 COMMENT 0.082322 1) COMMENT #235=(CLabel (CWidget 0 (0 0) 1 2 0 0 #164# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (4923)" "@N (@S)" 5 -13.5965 COMMENT -5.22699 0 -10 4.32092 0.799245 #234#)) COMMENT (CObjectList)) COMMENT #236=(CGroupWidget COMMENT (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -660 100) COMMENT (CObjectList COMMENT #237=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #162# 649 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.00541 1.9 COMMENT 0.082322 1) COMMENT #238=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 13042376 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Hpa}I (2944)" "@N (@S)" 5 12.0378 COMMENT -8.71165 0 -10 4.24599 0.799245 #237#)) COMMENT (CObjectList)) COMMENT #239=(CGroupWidget COMMENT (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1288 100) COMMENT (CObjectList COMMENT #240=(CScratch COMMENT (CWidget 1 (1 #5# 1) 1 2 0 0 #162# 712 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.99385 1.9 COMMENT 0.082322 1) COMMENT #241=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 29763380 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xho}I (2959)" "@N (@S)" 5 12.0593 COMMENT -10.454 0 -10 3.97125 0.799245 #240#)) COMMENT (CObjectList)) COMMENT #242=(CGroupWidget COMMENT (CWidget 1 (10 #8# 0) 1 2 0 0 Nil -1009 100) COMMENT (CObjectList COMMENT #243=(CScratch COMMENT (CWidget 1 (1 #8# 1) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.97613 1.9 COMMENT 0.082322 1) COMMENT #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #164# 39 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Not}I (2982)" "@N (@S)" 5 11.9919 COMMENT -12.1963 0 -10 3.79641 0.799245 #243#)) COMMENT (CObjectList)) COMMENT #245=(CGroupWidget COMMENT (CWidget 1 (10 #9# 0) 1 2 0 0 Nil -940 100) COMMENT (CObjectList COMMENT #246=(CScratch COMMENT (CWidget 1 (1 #9# 1) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.55154 1.9 COMMENT 0.082322 1) COMMENT #247=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 29694188 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Nhe}I (3533)" "@N (@S)" 5 8.23299 COMMENT -17.4233 0 -10 3.79641 0.799245 #246#)) COMMENT (CObjectList)) COMMENT #248=(CGroupWidget COMMENT (CWidget 1 (10 #11# 0) 1 2 0 0 Nil -800 100) COMMENT (CObjectList COMMENT #249=(CScratch COMMENT (CWidget 1 (1 #11# 1) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.25045 1.9 COMMENT 0.082322 1) COMMENT #250=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 24629308 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (2626)" "@N (@S)" 5 14.2779 COMMENT -1.74233 0 -10 3.97125 0.799245 #249#)) COMMENT (CObjectList)) COMMENT #251=(CGroupWidget COMMENT (CWidget 1 (10 #12# 0) 1 2 0 0 Nil -1224 100) COMMENT (CObjectList COMMENT #252=(CScratch COMMENT (CWidget 1 (1 #12# 1) 1 2 0 0 #162# 514 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.98826 1.9 COMMENT 0.082322 1) COMMENT #253=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 36191052 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xma}I (4264)" "@N (@S)" 5 -5.13014 COMMENT -12.1963 0 -10 4.32092 0.799245 #252#)) COMMENT (CObjectList)) COMMENT #254=(CGroupWidget COMMENT (CWidget 1 (10 #15# 0) 1 2 0 0 Nil 1024 100) COMMENT (CObjectList COMMENT #255=(CScratch COMMENT (CWidget 1 (1 #15# 1) 1 2 0 0 #162# 449 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.99519 1.9 COMMENT 0.082322 1) COMMENT #256=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 24110188 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Bsr}GI (4255)" "@N (@S)" 5 -5.15501 COMMENT -13.9386 0 -10 4.14608 0.799245 #255#)) COMMENT (CObjectList)) COMMENT #257=(CGroupWidget COMMENT (CWidget 1 (10 #17# 0) 1 2 0 0 Nil -1572 100) COMMENT (CObjectList COMMENT #258=(CScratch COMMENT (CWidget 1 (1 #17# 1) 1 2 0 0 #162# 30717496 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 6.08825 1.9 COMMENT 0.082322 1) COMMENT #259=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #164# 37620572 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Mlu}I (241)" "@N (@S)" 5 8.57301 10.454 COMMENT 0 -10 3.34684 0.799245 #258#)) (CObjectList)) COMMENT #260=(CGroupWidget COMMENT (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -1079 100) COMMENT (CObjectList COMMENT #261=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 6.26548 1.9 COMMENT 0.082322 1) COMMENT #262=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #154# 639 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Sal}I (11)" "@N (@S)" 5 2.65061 12.1963 COMMENT 0 -10 2.57257 0.799245 #261#) COMMENT #263=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 1.93643 1.9 COMMENT 0.082322 1) COMMENT #264=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #154# 30101484 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Sal}I (5629)" "@N (@S)" 5 -14.1972 0 0 COMMENT -10 3.62158 0.799245 #263#)) (CObjectList)) COMMENT #265=(CGroupWidget COMMENT (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -1079 100) COMMENT (CObjectList COMMENT #266=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #162# 895 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.90215 1.9 COMMENT 0.082322 1) COMMENT #267=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #154# 36372828 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Age}I (3078)" "@N (@S)" 5 11.6109 COMMENT -13.9386 0 -10 3.87134 0.799245 #266#) COMMENT #268=(CScratch COMMENT (CWidget 2 (1 #6# 2) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.54461 1.9 COMMENT 0.082322 1) COMMENT #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #154# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Age}I (3542)" "@N (@S)" 5 8.20857 COMMENT -19.1656 0 -10 3.79641 0.799245 #268#)) COMMENT (CObjectList)) COMMENT #270=(CGroupWidget COMMENT (CWidget 2 (10 #14# 0) 1 2 0 0 Nil -735 100) COMMENT (CObjectList COMMENT #271=(CScratch COMMENT (CWidget 1 (1 #14# 1) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.51147 1.9 COMMENT 0.082322 1) COMMENT #272=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #154# 31595476 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Msc}I (3585)" "@N (@S)" 5 7.87675 COMMENT -20.908 0 -10 3.79641 0.799245 #271#) COMMENT #273=(CScratch COMMENT (CWidget 2 (1 #14# 2) 1 2 0 0 #162# 712 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.65383 1.9 COMMENT 0.082322 1) COMMENT #274=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #154# 1095193665 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Msc}I (4698)" "@N (@S)" 5 -12.4881 COMMENT -6.96932 0 -10 4.04618 0.799245 #273#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #275=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -596 100) COMMENT (CObjectList) (CObjectList)) COMMENT #276=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -247 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 35542972 COMMENT (CStringList) 35543032 35543008 (CObList))) FEATURES Location/Qualifiers misc_feature 1076..1213 /vntifkey="21" /label=Psi misc_feature 1707..1910 /vntifkey="21" /label=RRE misc_feature 2944..2958 /vntifkey="21" /label=shRNA\Cloning\Site promoter 2631..2943 /vntifkey="29" /label=U6\Promoter misc_feature 2825..2858 /vntifkey="21" /label=loxP misc_feature 4941..4974 /vntifkey="21" /label=loxP misc_feature 5030..5409 /vntifkey="21" /label=WRE primer 2481..2500 /vntifkey="27" /label=Lox\Replace\For\Primer primer complement(493..512) /vntifkey="27" /label=Lox\Replace\Rev\Primer misc_marker 3554..4261 /vntifkey="22" /label=mCherry promoter 2989..3531 /vntifkey="29" /label=EF-1alpha\promoter misc_feature 4325..4918 /vntifkey="21" /label=Puro misc_feature 4268..4321 /vntifkey="21" /label=T2A misc_feature complement(7151..8008) /vntifkey="21" /label=AmpR BASE COUNT 2020 a 2102 c 2157 g 1862 t ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2821 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 2881 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 2941 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 3001 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 3061 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 3121 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 3181 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 3241 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 3301 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 3361 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 3421 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 3481 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 3541 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 3601 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3661 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3721 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3781 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3841 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3901 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3961 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 4021 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 4081 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 4141 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 4201 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 4261 gccccgggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 4321 tcgaaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 4381 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 4441 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4501 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4561 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 4621 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 4681 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 4741 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 4801 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 4861 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta 4921 agaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt 4981 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct 5041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5401 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 5461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5581 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc 5641 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 5701 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 5761 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 5821 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 5881 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat 5941 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag 6001 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg 6061 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg 6121 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 6181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6301 tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga 6361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 6841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8101 taggggttcc gcgcacattt ccccgaaaag tgccacctga c //