LOCUS Ple93\(pEMS1161) 20225 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2649 - 5' CTGCTGAGTGCAACCTTGAAAATC - 3' Tm=56.9 Deg C Antisense primer: oEMS2619 - 5' CCCAGGACTGATCTCTTTATTCCC - 3 Tm=56.1 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 517 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543236896| COMMENT VNTDBDATE|544977165| COMMENT LSOWNER| COMMENT VNTNAME|Ple93 (pEMS1161)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#207=(AnlzFontBackColorItem 2515 3914 33554687))) (CObList) 0 COMMENT (CStringList) 0 1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 14088..17899 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18998..20219) /vntifkey="22" /label=AmpR misc_marker 12637..14062 /vntifkey="22" /label=AmpR misc_recomb 8996..12641 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 5042..8995 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 14069..14086 /vntifkey="21" /label=I-Sce\I misc_feature 3924..3957 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3958..3968 /vntifkey="21" /label=Kozak\consensus misc_feature 3965..4684 /vntifkey="21" /label=EGFP misc_feature 4685..4720 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4721..4793 /vntifkey="21" /label=NLS misc_feature 4799..4832 /vntifkey="21" /label=MFRT misc_feature 4986..5036 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3915 /vntifkey="21" /label=Ple93\(GPR88-B) /note="1306" primer 2761..2784 /vntifkey="27" /label=oEMS2619 primer 2268..2291 /vntifkey="27" /label=oEMS2649 misc_feature 139..1161 /vntifkey="21" /label=Region\1 misc_feature 1162..2515 /vntifkey="21" /label=Region\2 misc_feature 2516..3915 /vntifkey="21" /label=Prom\Region BASE COUNT 5528 a 4465 c 4437 g 5795 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccta tgaattaatg aacctgtttc acagggcaga aggaaatctt 181 tttttgtaca gattgggttt ttatgaaaaa gaaagtaaaa aaaacaaatc aattagctgg 241 tttcagcaga tttctcacct attgagttta cctgtttctt ttttatcctt agatgtaaaa 301 tgatttataa atctcaaaac acacctggga aaactatcta tgtgattcaa atatagcagg 361 aacatgaatt taccaactat acacaaatgt attttgaaaa ttgtttttac ttctatgctt 421 tcttcagtga catgttaaac cattaatatg ttgagtctaa ttaaaccagt tgttagtata 481 taagctgaac aggactccgt gggcttcatg tcatataaga agtctgtact cgttccaaaa 541 gtcataaggc agagtcccct ttgcaacact tagcaaagca agaatttggt cactgtatgt 601 ctcctcagaa gattaattag ggacaattcc aattacagaa gtatataatt tacagcagaa 661 aactccttta tttacacata ggttgcatag ggttgacaac tatggcatat aatggaaagt 721 agaaaataat tgtattaagt tattttgagc tgaattgaaa tgttaatata ttcccaccca 781 gttccctctc tatggattga attatgataa tcatatcaag aaggagaaaa atagcttttc 841 tcaagaattt taaacttttc tttgctaagt acaagtagaa acttacatta aaagactagt 901 tttaatgcga aatgtatttg gaacaggttt taagtggaca aacataaatt agctgaactt 961 ctcactctgc atgcctcgtt ttaacctgca cgccaccaaa atcaaagtaa atcacatcac 1021 cacttgcggt gtctgctttg gtttgaatca agttgaccat gcatttttga gaagttgtac 1081 tccaagtgaa gaatggggca tggtaataac tgtagaaatg actctacctg caaatattta 1141 cattaaaact taagatttgg gaataggggc tttggacatc gaatttcaag acaactgaca 1201 ctgagacttg aagctgagat aaagaggcct ctcagacatc acaggagatc tgtcctttct 1261 ttccagactg ggcatattta tgccaaacac acagttttag tcaattccag gactcttcat 1321 tggcactcac tttactcagt tttgaagaaa agattatctc caagtgagat tgccagtctt 1381 gatattcaga tccccaaaaa taattatgtc gagtacgtgg aaggacaaaa ttggaaccag 1441 agatcagatt actataagaa tccatctatc tatctaccta ctactctcct accaacctac 1501 catataactg ggctttacta gagctaatgt aagtataaca tatgcctttt tgtattggga 1561 agttaaataa aacttggaag acacaggggt cccacagact tgagaagaaa ggagctgatc 1621 tttgtaaaaa acaaaaccaa aaatgaataa aacaatggag gagagggagc cattttttaa 1681 aatctaggtt ccttacaggc aaagagatat tttgttttaa atggaaaaac ataaattgga 1741 taccatactt caagaagcat gttccaggca gttttatcaa ctctggaaag aggtaaccag 1801 ttaacttttc aagcagcttg gggttgtaaa gtaatgttgc atctgctgag tgtgaccagc 1861 aggattggaa agggaggcct agctttttct gatttgaggt tttgtgtaat actcacttcc 1921 tctttctccc ttctccactt ttcataccaa tgagtttcac aagcatagcc accaatccta 1981 gacttttcct gctgcttgag gcccagctgg tagcttactt taccattttg gtgccttcaa 2041 tgaccaacaa ctaaatggcc gggcaccacg tcagatggca acactgcacg aactatacag 2101 ctaacatctg ttagcagaga cactggtggc aaaatccctg gtctcagtct cgtatcacat 2161 ccccatagag caccagcccc ggagttctct ttctctcata ttgctctgga gggtgaatca 2221 gagatgctgg ccctgagatg gctgccctgt catgacaaga agtgggactg ctgagtgcaa 2281 ccttgaaaat ctactaagct gacaaactgt gactttagtt tgatctattt taaagtcaaa 2341 tctgttgggg caaattatcc ttaattaagg atttctgttt aagtgacaag tagttaggat 2401 tgttaatttt taaggtaaac caaaagagga aaagttcact catctggtct ggaggagaga 2461 tgaaagtcag ttactaagtc aggatctaat tatttatcta atcaggaaag ccctgtccat 2521 tgtctagatg taccactgac atctgcttta aatcttttgc ccatttcatt tttaccctct 2581 aaccatttca ttactaaaac acaaaacaaa ctccggaaag gagtttgttg atgagaaaac 2641 actgaaagta cgatttaaat tgaggaatgg agattttctg tggataattg tctatgttcc 2701 actttaccaa agaaaaatga atttcaagta gtgcataaag taattctcgt taaaaaatgt 2761 gggaataaag agatcagtcc tgggtactgg gaatatattt atctcacaga aaaatcttgc 2821 ccacaaaatg catcagactg agatgaaata ctctagggta tattaaattt tattctaata 2881 ttgaagggag ggaaaaaaag gacggaaaga aaaatgaaac aggatgaaaa ataaggtgat 2941 tgggagagag aaaagacaga gaaacttgtt aacgacagga ctttctgagc aagatatttt 3001 tcttcctttt acaagttgga agctattgaa taattcatgc caagcgtgga aaggctgtct 3061 tttccctgga tgatgtataa ctaagcagga gagagcttcg agtgggttca ccacatcagc 3121 caccactcct gcttctgagc acagggtgct ctcctcttga gctcagcttc tgcttttgca 3181 gccaagcatt cttgctgctg ctgcctgcct gcccacccgc ctgggcttgc agcccgccac 3241 tttactttct ccagccctga taccagctga gaagtctccc tgcagctgct agttcctgcc 3301 caggaccatg tgtgtggatg ctgcttggga gaagcgggca cttgctcctg gcactgatcc 3361 cagctgagtt tctcctgttg atttctggac cactgatgct gttgctgagg aggtatttcc 3421 cggcatccct ccccctgaga caccggctaa ggaccagcct aaacgcaagg taaccttcac 3481 ctctttccca tgggattttc cccagctggg atatctctgc tttctcctgg aactaggtcc 3541 tccagctggc aacatatctc tctctctctc tctctctctc tctctctctc tgtgtgtgtg 3601 tgtgtgtgtt gggggagaag aggagactgt tttaggtatt gctctgtcct agggcttccc 3661 cgatacctgc tgaatccaat gcaaagtggc ctttcccctg gctctggaga catttccgag 3721 gcaggcacct gtcatctgcc acccccgtcc tcctcctcga gctccctttc tccccctccc 3781 ccagcccatt attctgcttc agccttttgt gtcagtggca gagggctgaa gggatgtctt 3841 tgcccttctg gcaggcagga cagtgtcagg atggaccgcg ctgccagaag ccgacgctag 3901 cgagggaggt gtgaaggcgc gccgaagttc ctattcttca aaaggtatag gaacttccgc 3961 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 4021 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 4081 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 4141 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 4201 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 4261 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 4321 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 4381 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 4441 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 4501 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 4561 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 4621 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 4681 gttatttctg aactgctgcc cgggctgctg catggaaccg gatccaaaaa agaagagaaa 4741 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taatataaga 4801 agttcctatt ctctagaaag tataggaact tcagcggccg cgactctaga tcataatcag 4861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 4921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 4981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcgaat 5041 tctcgtaaaa ctcttcatgg cagtagttat tattctctct ctctctcttt ttcttttttc 5101 ttgagacagg atatttctct gttgcccagg ctggagtgca gtggcacagt cttggctcac 5161 tgcagcctgg acctcctggg ctcaagccat cctcccacct cagcctccca agtagctggg 5221 gctacaggca catggccacc aggcccagat aatttttcat ttttgtagag actgagtctc 5281 accatgttac ccaggtttat tattctcatt ttttagatga agagactgag gtccagagaa 5341 gctcaatgac ttgcctagtt ttacaaatct cctgccatca catacccctc agcgtcctta 5401 ataagaggga ggccaccaac tatgtgctgg gcactgtggt ggatgctgga gctatagggt 5461 tgagtatata agaaatggtg ttgctggagc aactgttgct tgcttacctg acctatctga 5521 gaattaatta gcaggggaac atatttttgt tttcagattc aatataagaa cttgtgtggg 5581 caaaaataaa gatcagtagt aataacagta gttcccattt gctgactgta ctgtcctaag 5641 tgcatatata tatacataca cacacgcata cctatactcc tctaatactc aaaatgatcc 5701 tgtttatgta ttgttaatat gctcatttta tttttaaatt tttatttatt tttattttta 5761 tttatttttg agacggagtc tcattctgtc gcggaggctg aagtgcagtg gtgcgatctc 5821 agctcagtgc gacctccgcc tcccgggttc aagtgattct cctgcctcag cctccggact 5881 agctgggatt acaggcgccc gcctccacgc ccagctaatt tttgtatttt tagtagagat 5941 ggggtttcgc catgttggcc aggctggtct cgtactcctg accttgagtg atccacctgc 6001 ctcggcctcc caaagtgctg ggattacagg catgagccac cgcgccgggc taatatgctc 6061 attttagtga ggcaaaaata gaggctcaga gtctgatttg tacaaaacta cagagcagtt 6121 aagtgtcctc tcagatgtgt accctgatct gggtgactct aggactctag gtctcaactg 6181 ttacaaccag ttaagggttt ggggaagcac tgggccaaga gtcaggaaaa tggaagccac 6241 aggtagtgca aggtcttggg aatgggacgt ctggtccaag gattcacgcg atgactggaa 6301 cccgaagagc cggggcccgg tttacggccg ccatgaagca acgcgcgccg gtaggtttgg 6361 gaatcaggga gccctctgaa taggagactg agttgggagg gaaaggggct tcgctggggg 6421 agcctcggct tcttctggga gaaaattccc acggctacct agtgagcctg caaactggta 6481 ggcgccggcg taggcgcgcg ggcggggccg ggggcggggc ctgcggggcg tggcggggcg 6541 ggcagagggc ggggcctgct tctcctcagc ttcaggcggc tgcgacgagc cctcaggcga 6601 acctctcggc tttcccgcgc ggcgccgcct cttgctgcgc ctccgcctcc tcctctgctc 6661 cgccaccggc ttcctcctcc tgagcagtca gcccgcgcgc cggccggctc cgttatggcg 6721 acccgcagcc ctggcgtcgt ggtgagcagc tcggcctgcc ggccctggcc ggttcaggcc 6781 cacgcggcag gtggcggccg ggccctgagg cgcgggatcg atccatgaca cccccccgcc 6841 ccccatatct cactataatt cactgtcaaa tggcttttat gatttggagt ttggggatac 6901 ttttaaagac agggtcttgg tagctcctag atggcttggg attcacttag taacccttgc 6961 tggcctaaaa ttcaggaact tctacctcat cttcccaagt actgggttag tagggatgtc 7021 cagcatattt agcttgcttt aatgattttg ctgtactttt ttctgtgtgt acaggtgcat 7081 gtatatttat aaaactaaca taatatattt tacccccaaa gtaccataac cctaattaat 7141 gctattaaat gttttgtatg ctgggctctg ggcatactaa tataaccaat attatacaat 7201 gcaaaacaat tttggaatat cttctgtaaa attatcttat tagtaagttg taaccatctc 7261 agacttctaa gtttgagttt gtaaaggatc agtttttttt ggtgacctac acacacacac 7321 acacacacac acacacacac acacacaggg gggtggattc tgagaattta gagaatttag 7381 atggttaata ccagttttta tttttattct gtgtgatatt tatttattaa gatttattta 7441 tttattacat gtaggtacac tgtatctgtc ttcagacaca ccagaagagg gcatcagatc 7501 tcgttacaga tggttgtgag tcaccatgtg gttgctggga tttgaactca ggacctctgg 7561 aagaacagtc agtgctctta accgttgagc catctcacca gccccgtgat attttattta 7621 acttactaga atatactgtg ttaaaacata ttttagggga ttggggagat ggcccattct 7681 gtaaagtgtc tgccatgtca tcttcaggac ctgcgtttgt aaccccagca tggagaagac 7741 agagacaggg agacccctgg gcttcctggc aagctagact agcaaatttg gttaactagg 7801 ttcaatgaga gactcagtct caaaacataa gaagtgaatt ttttagaccc aatttcaatc 7861 tctgaactcc atacgtatgc acatgtacct gcatataaat atatatacac acccataaac 7921 acatgcacac acaaaaaatg gttcaaaagt tcttaaaatg tataagagta gaaagtgtag 7981 agccgggagt ggtggcgcac gcctttaatc ccagcacttg ggagcagagg caggtggatt 8041 tctgagttcg aggccagcct ggtctacaga gtgagttcca ggacagccag agctatacag 8101 agaaatcctg cctcaaaaaa ccaaaaataa agtgtatata ctaatctttc atgcctaaaa 8161 tatttatttt tgccacattt ttcatacctc tttaatattt ttctttttct ctttgtgctt 8221 caaaggaaat tttgtgtcat gttttacttt tcattcttga ctctattcag cagtaagacg 8281 cagcatggct ttcttagaga agtataatct attacaaaac ggtaacattt aactgctcta 8341 cataccctct tgtagctaaa ttcctctgtt aaactaagac ttattatcta aacgtaatta 8401 aatgttgata tcttataatg ctccactttg aaacagctgt ctttcttttg cagattagcg 8461 atgatgaacc aggttatgac ctagatttgt tttgtatacc taatcattat gccgaggatt 8521 tggaaaaagt gtttattcct catggactga ttatggacag gttagtaaga cctcgattga 8581 agttattttt agagggtgtg tgtgtggtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 8641 gtgtgtaaga gagagaggag aggcatgtgt gatatatatg tgaagtgtat tgcatgtaca 8701 tggaatccag agaaggacat taggtgtcct actctgttgc tctcttattc ccttgaatca 8761 gtgtctcttg ctgaacctgg ggcatacata ctgatttggc taggctgcct ggctagcaag 8821 acccagggat ctgcttgtct ctgcccccca gtgctgacgt tacaagcagg tgcaaccaca 8881 tctggttttt atatgggtac tgggggatct gaacttaggg cctgatgcgt gcccaagtgc 8941 tctcacccgc caagccacct ctccagccaa agggtttttt ttcttgtatt aattctaact 9001 tagcaactgt tgagtagttg atgtttgaat ataagctaag ccaacttatt agaataaatg 9061 ttttctattt tttacttgta tgtgtgagtg cctgcatgta tgtatgtgaa gcatatgtat 9121 tccaggatgt cagaattgtg atggattccc tggaactgga gttacagacc attgtgaacc 9181 actgtgtatg ggtgctggga actgaacttt aggtcctctg caaaaacagt aaggatctta 9241 actgataaac tatctttcta gccctaaggc tttctacttt taaagttcgt ttctctttct 9301 ctctctctct ctctctctct ctctctctct ccctctccct ccctcctcct cctcctcctc 9361 tttcccccct ctcgcctcct ctttctctct cccctccctc tctctctctc acacacacac 9421 acagacagag agaggagaga gaaaggaagc cttactaatt aattacctag ttttagacat 9481 aactagtggg aatccatagt aagtaaatca acattcttga agagaggtta gaagaattgg 9541 ctctcgttgt ccatcgaggc tgtataagat tattcttctg ttttctcagc ttaccataca 9601 tattttctac gtagacaata ccaattcaaa tacactgtac gtaaagtata tgcgcacaca 9661 cacacacaca cacacacaca cacacacaca agttacatat tgtcatatag tactaaaatt 9721 tatggacttt ttcatattaa tacatctaga gctatcatgt tttttatctt ttaaaaatta 9781 ctttacgggg ctacagagat gtctcagcag ttaggagcac tgactgctct tccagaggtc 9841 ctgagttcaa ttcccagcaa ccacatggtg gctcacaacc atctgtaatg ggatctgatc 9901 cactcttatg gtgtgtctga agacagtgac agtgtactca tatacatgaa ataaatcttt 9961 aaaaaattac tttaccatca gggtatatat atatatatat atgtgtgtgt gtgtgtgtgt 10021 gtgtatgaag tatttgctat ttgagagggt ctcactgttt agctcagtct ggcttggaac 10081 gttctgtata gcccagactg gcctcaactc agcagtcccc ctgaaaggct ctcatatgtg 10141 gagattacag gtatgtgcta ctgtgcccag ccctacatgt tactttgttt ttgaaatagg 10201 gtatcaaata gcttagactg gcttctaagt cagtgtgtag ctaaggatgg tctgagcttc 10261 tgaacctgct acttcaactc ctggtgtgcc atcatggcca gtttgcacag ttgtgagagt 10321 agaactcagg acctcctatg tgttactaga catgttctct accagctgag ctacatccca 10381 attcgcctac atttactttt attttttaaa agatttattt atgtatacag cactctgcct 10441 gcatgtacat ctgcatgcca gaagagggca ccagatctca tcatacatag ttgtgagcca 10501 ccatgtggtt gctggggatt gaactcaggg cctctggaag agcagtcagt gctcttaacc 10561 tctgagccat ctctgtagcc ccctacattt attttaaaga ttataggcta ttctattcat 10621 agatgtgcca taactgattt aaccagatct tcattatttt gtacttagta gagtatatta 10681 gagggaatga atcttaaata cacattttga cacttgctca gctttagaca agtaaaaatc 10741 actggtcaag gaaatggtgc ttagtggatg cttctgaact gtcaaattgt cctccaacaa 10801 gttacatcat tttatacttc caacgttatg tgagagttca tttttcttta ggctcttcta 10861 gtattttata tttgagaaat aaaatttaac caaaatttgc caatctagta agcttgtttt 10921 tatttgtatc tttaaatttt atgatagatg ttatagtgta ctctcctctc ccctcccctc 10981 ccctctcctc ctcagcagga tcttgttatg tagccctaag ggatctaata cttactctgt 11041 agtccaggct gtccttcagc tcatgagaca caccatcatg cttggcatgt ttcttgttct 11101 gaaaggtatc tggttttaat tttggttgag ctggtgtgtt ggtgtatatc tgtaatctca 11161 gcacaggcaa gtagagacaa ggattatgat tacagtccag cctgggctat ttagagggat 11221 tctctttaat ctttcctcat gccccaaaat cttacctttg gtatatgaaa aatagtctcc 11281 acttctgcaa aatattgctt tatgaagtaa gaattaattc ccttcataga gacaaggaat 11341 gtgtcctgta aaagtttaat gtgtaagaag tatttgttat aaaagataaa tattcagaat 11401 cttcttttta attcctgatt ttatttctat aggactgaaa gacttgctcg agatgtcatg 11461 aaggagatgg gaggccatca cattgtggcc ctctgtgtgc tcaagggggg ctataagttc 11521 tttgctgacc tgctggatta cattaaagca ctgaatagaa atagtgatag atccattcct 11581 atgactgtag attttatcag actgaagagc tactgtgtaa gtataattaa cttataatta 11641 aaaaaatagg gccattctag ttttatctat atttttttta aacttgtgca aactatgcta 11701 cataatcaac ttacttgatt atgtaatata tatagtaata gttggaatta ctattattac 11761 atatttattt ttctgtgctg gggtagaacc cagggaacta agtactgtaa taaccgagct 11821 tcattcatcc tagtcatttt taattttttt tttttttttt gttttttcga gacaaggttt 11881 ctctgtgtag ccctggctgt cctggaactc actctgtaga ccaggctggc ctcgaactca 11941 gaaatctgcc tgcctctgcc tcccagtgct gagattaatg acgtgcacca ccactgcctg 12001 gcattttttt aaagttttaa tttgagaccc tttctcacta acttgctcac actatcctcg 12061 aactcattac gtgggctggt ctagaaattt cagtcaactt gcctcagctt ctcaagtagc 12121 tgggattaca ggcaggtgct accacttctg gccatgatca ttgttgttgt tgctattatt 12181 attattatta tttcactgat acttcatatc acaacacaga atagtaactg tagttcatga 12241 ccaacaaaaa agttgtacag tttctctgtt gtcttatttt gatctggatt ttgatttgtt 12301 tcatggcact gggcaggcta atctcaaact gcatggctca aatagtcctt ctgtcttaat 12361 ctcccatgtt ctggaagtat aggtatcact acacctagct gtattttcat attttaaaaa 12421 aaacgcctaa gaattgctat tgaatgaggc ggagagatgg ctcagcagtc acaagtactt 12481 gtagaggact ggggtttggt tactagcacc tcaaagatgg cttacaatta tctgtagctc 12541 caattccaga ggatctgtgt ggtaaagctg catgtatatg ggcatgcagg taaaactgct 12601 aaacatataa aaatagctca gtcttaagag aattggtcga ctttttcgcc ctttgacgtt 12661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 12721 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 12781 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 12841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 12901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 12961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 13021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 13081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 13141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 13201 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 13261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 13321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 13381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 13441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 13501 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 13561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 13621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 13681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 13741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 13801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 13861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 13921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 13981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 14041 acaaaaaaac caccgctacc aggtcgagta gggataacag ggtaatggat cctcgttggg 14101 cacctgcact catgtacgca ggcccacata tagatgtaca cacgcatatc attcaaaata 14161 gaaattattt atacaagtag tgcgttggag agctggctca ggagttgtta atgcttgcta 14221 cccttgcaaa ggacccaagt tcaatttcca gcacctgtgt tgggcatctc acaaccacat 14281 gtaaatctag tttcagggga tttcacccct cttctgaact cttcaggcac ttatacccac 14341 aaatggacat ctccacatag agacacatac ttttaaaaaa gtaatctagg gtctagagaa 14401 atggttatag accacggact gggatcagtt cccagaagcc acatcctgtg gctcacaatt 14461 gcctgtgaat caagttctag atctggtggc ctcgtctagc ctgcagtcat caacaaacac 14521 atgcacacat ccatacacac ataatttaaa agaaagcgaa agtaacctat aatgctaaac 14581 aaagcacatt ctcagtgtag actgcagtgg tgatgaacct tgaaaatata ttaatagaag 14641 ccagacacgt aagtcacaaa tggtataact caatttatat gaaattccca gagagtccaa 14701 agttaaaaaa aacaaaaagg cagattattg gttgtcagta gctacagaga tgggacattg 14761 aagatattaa taggtatagg gttgaggatt catggccttt ccctcttcca tgttagcatg 14821 tctattgttg ttaaccttat tcaactcatg ttagggcaat catattgatg agacttaatg 14881 agtatagctt gacattccta gaagacagag tctcacatca gactctctga tcctctgact 14941 cttagaatct ttcaacaccc tcttctgaaa tgatctctga gcctttggtt gtgactgaat 15001 tatgagaaag aattttatgt tgaatgaatt ttacttcagc atttttacaa aactgaagct 15061 ggggatggcg tttcatgcgt gcagtcccta ccatttgcct ataagcctgg aggaggcaga 15121 agtagaatta ggcttttcag ggacatcttc agctgcatca tgagcttgag gacaacttga 15181 gctacataag attcccatcc ccacccccac acacacacac acatctagta tgtacctagt 15241 atgcagtctt ttcatatgtt taaaatgtag aaaggtatat gtgaagtctc ccttttcccc 15301 acaacttagt ccctccaaag tattgcatca gtttccttgt acatgatttt atgcatatat 15361 aagatacact atttctctct ctctctctct ccttccctcc ttccctccaa cctctgggta 15421 tgagttgagg gttatgtatg tttcaagttg taataagaat tgccactcaa acatttcatc 15481 ccattttaag ttaagtttgt ctgattttcc actttagaag ggcagccatg atggctcatg 15541 cctataatcc catcctagca cttgtaagcc taatacataa gactggagga ttgtcatcag 15601 cttgaggcta gcctggtcta cctaatgagt tccagccagc caagagtaga atataagacc 15661 ctgtctccaa aaaatgtacc aaaggggctg gaggggtaac tcagtggtta agaacagtag 15721 ctgctcttcc agaggacctg ggttcaattc ccagcaccca catggcagct tgcaactatc 15781 tgtaactgtc cagctttggg atatccgaca ccattatacc aattacataa aatgaaaaaa 15841 ttgtaaaatg tgctaaaatg agtggggagg gggagcatgg tgtggttctg tgggcttggg 15901 attttgccat gaatccatga ctatatctca gcaatatgtg gattatttct ggaaacctgt 15961 ggggcatggg tgtatgggaa gaggggagag agagagagcc cgcatccagc cagagttcct 16021 gtgctctggg caggcggacg tgggaggact gctggacgat ttccacttgg ccctgggtgg 16081 gcatctggct gtgtgaagcc actgacccca ctcagcgggg ggtagataag gggcatttcc 16141 cataccaggt tctcagactc cagcattcca cgctggatac agcagaagag ctgagaacag 16201 aataggggcc ccagggagac actctcgtcc cagagataaa gaaggcagag ggagagagat 16261 tcctacgcag gcgagaggcc ttagtctggg ccttgatgaa cagagacggt ctatggtttt 16321 agagctttat tgtagaaagg cagggggaaa gagagaaggt agaaagagag agaccagcca 16381 tggccaagag gagagaaggg gggaaaggag agagagaaga aaggctagag agtaagaaag 16441 gttaaagaga cagaagtaaa agcttagagt aagagagaga gagagagcaa gagggagagt 16501 aagaaaagta aaagcttaag agagcgagga ggggccagcc cctctaatag tgggcttgtt 16561 atcttgttgt tgctaggtaa ctgtgaagcg tctagcctga aggccagaag cttgggacat 16621 tgtctatgtg actgaaagcc atgagcctct actgtggggg ctgtggaggt ggtaacttgg 16681 acaggagcca ggggttccag gagacatgag ggaatgcctt ctgtcccatg taggtaggaa 16741 ttaccaccac cggctttcac cgtagttcac tggagactgt ttgtacatag cccattgccc 16801 cacagaaacc aggcactcac tgtttcattt gcataagaat tagaaggctt tattttggct 16861 gggattggtg acttgcccca gaaattccag cttttgggaa gccgaatcct ggagaaccag 16921 gagttcaagg tcatcctcag gtacctcatt tgttcaagac cagcctgtgc taccatgaga 16981 ccttgtccct actcccctca accccaaaac acgctgtttt cattttatag gttgtactaa 17041 ccatggagga tggtcatttg cctttctctt gatttcagaa gcgtagcgcg ggctttgcac 17101 catgatctag tgtttccaga taggcttgcc tctccacagc caggcttatc ctgcttcagt 17161 cccatgtttg gcaagggcaa catatgactg aagaacttgc atagccttat ggtgtatcat 17221 gactcttagc cccagtttat aactgtctaa cagctggtgg cttttgtgaa aaaggaaagc 17281 atcatttgaa cttttaactt ttcagctgtt tgttttgact ttgcataaat actgagacta 17341 gctaggaaat gagcttttgt ttcatttgct aaaaatgatt tttttttcat gaggcaattt 17401 caagcaattt gaatttagca atctctactt gtctgaaaag aagttattaa ttaaaaaaaa 17461 acaaacaaac aggaacctat tatgctggct agtcacatga aagaatccaa atgaacacag 17521 tcaaggctga atcaacacat gaaattattt tctaattccc caaattacat ttatgctaat 17581 tagtatgtag taaaacattt taattgtttc caatgttaaa cattacagaa ataattttga 17641 tgttttccta ataacccagc ctttgatcat gtttgcattt atttgattgt tgtcagttta 17701 ttaaataatt aagtaagagc aacaagataa aatgacttca tgagagtcct tttctgtaac 17761 ttagaacgtc agtagtcata ggaactgcgg tcgttccctt acctttcaga agtaggctaa 17821 agagttgaac gcaaaggtgg tttttcgaat cttcctgatc cttaagcccc cactaggttt 17881 atattctata tttgatatcg gattcgaacc cctcgagctt aggatccgga acccttaata 17941 taactaagtt attaggtccc tcgaagaggt tcactagtac tggccattgc ggccgcttcg 18001 aggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 18061 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 18121 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 18181 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 18241 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 18301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 18361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 18421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 18481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 18541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 18601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 18661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 18721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 18781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 18841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 18901 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 18961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 19021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 19081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 19141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 19201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 19261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 19321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 19381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 19441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 19501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg 19561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 19621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 19681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 19741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 19801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 19861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 19921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 19981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 20041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 20101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 20161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 20221 gacgg //