LOCUS pAAV\CB6\FFLuc-m 6341 bp DNA circular 24-FEB-2012 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|618693107| COMMENT VNTDBDATE|618693107| COMMENT LSOWNER| COMMENT VNTNAME|pAAV CB6 FFLuc-miR122| COMMENT VNTAUTHORNAME|JunXie| COMMENT VNTAUTHORTEL|508-856-3316| COMMENT VNTAUTHORFAX|508-856-1552| COMMENT VNTAUTHOREML|jun.xie@umassmed.edu| COMMENT VNTAUTHORAD1|University of Massachusetts Medical School| COMMENT VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250| COMMENT VNTAUTHORAD3|Worcester, MA 01605| COMMENT VNTOAUTHORNAME|Sheena Saighal| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/vntifkey="33" /label=Origin\of\replication repeat_region complement(3391..3520) /vntifkey="34" /label=3'\ITR repeat_region 1..130 /vntifkey="34" /label=5'\ITR polyA_signal 3176..3302 /vntifkey="25" /label=Rabbit\globin\poly\A enhancer 198..579 /vntifkey="9" /label=CMV\IE\enhancer CDS 1163..2815 /gene="luc+" /codon_start=1 /product="luciferase" /protein_id="AAA89084.1" /db_xref="GI:1200463" /vntifkey="4" /label=luc+ primer_bind complement(1164..1186) /vntifkey="28" /label=Primer_Bind_1 misc_feature 2840..3122 /vntifkey="21" /label=pri-miR-122 exon 2946..3011 /vntifkey="61" /label=pre-mir122 BASE COUNT 1574 a 1545 c 1603 g 1619 t ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 541 tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc cacgttctgc 601 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa 661 ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg gcggggcggg 721 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc 781 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg 841 cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag 901 tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg tgaggcactg 961 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt 1021 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact 1081 ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta 1141 cttaatacga ctcactatag gcatggaaga cgccaaaaac ataaagaaag gcccggcgcc 1201 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata 1261 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta 1321 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa 1381 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt 1441 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga 1501 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt 1561 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga 1621 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc 1681 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc 1741 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag 1801 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc 1861 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact 1921 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt 1981 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc 2041 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc 2101 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct 2161 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc 2221 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt 2281 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag 2341 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga 2401 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt 2461 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga 2521 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc 2581 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat 2641 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg 2701 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag 2761 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta 2821 gattccgaga tatcgggccc gactgcagtt tcagcgtttg gaaccactga ggagtctgtg 2881 acagacaacg gaggaatgtg aagggaaggt taataccctt gtgcctgaca gactttcctt 2941 agcagagctg tggagtgtga caatggtgtt tgtgtccaaa ccatcaaacg ccattatcac 3001 actaaatagc tactgctagg caatccgtcc actccacgcg tgacttgacg tctgttctct 3061 ccgagcaaga agttctgtct tgtgctagag ttctccattg tgtgtaatgt aaagtcgggc 3121 ccgcgatcgg taatgctcta gagtcgagga cggggtgaac tacgcctgag gatccgatct 3181 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 3241 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 3301 cggaagcaat tcgttgatct gaatttcgac cacccataat acccattacc ctggtagata 3361 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc 3421 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 3481 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac 3541 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 3601 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 3661 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 3721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 3781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 3841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 3901 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 3961 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4021 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4081 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4141 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 4201 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 4261 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 4321 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 4381 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 4441 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 4501 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 4561 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 4621 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 4681 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 4741 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 4801 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 4861 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 4921 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 4981 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 5041 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 5101 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 5161 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 5221 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 5281 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 5341 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 5401 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 5461 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 5521 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 5581 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5641 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5701 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5761 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5821 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5881 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5941 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 6001 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 6061 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 6121 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 6181 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 6241 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 6301 tgaccatgat tacgccagat ttaattaagg ccttaattag g //